Resumo Frangos de corte Hubbard com 320 dias de idade foram alocados aleatoriamente em quatro tratamentos (8 repetições, 10 aves/curral) e foram criados em condições de manejo padrão. As aves do primeiro grupo serviram como controle e foram alimentadas com uma dieta à base de milho, enquanto as aves dos três grupos restantes, ou seja, A, B e C, foram alimentadas com dieta basal suplementada com nanopartículas de cobre (CuNP) a 5, 10 e 15 mg/kg de dieta, respectivamente, por 35 dias. A suplementação de CuNP aumentou linearmente (P ≤ 0,05) o peso corporal (PC), o ganho de peso médio diário (GPDA) e o consumo de ração (FI) em frangos de corte. O ácido úrico, os níveis de glicose no sangue e a conversão alimentar (TCF) reduziram linearmente (P ≤ 0,05) com a suplementação de CuNP na dieta. A suplementação de CuNP na dieta também aumentou linearmente (P ≤ 0,05) peso, comprimento, diâmetro, índice peso/comprimento (P/L) e índice tibiotársico (índice TT) da tíbia. A inclusão de CuNP na dieta de frangos de corte aumentou linearmente os parâmetros medidos de músculo, ou seja; pH, diâmetro da fibra, área da seção transversal da fibra, diâmetro do fascículo, área da seção transversal do fascículo (P ≤ 0,05). A concentração de cobre, ferro, cálcio e fósforo no sangue também aumentou linearmente (P ≤ 0,05) com a suplementação de CuNP. No geral, CuNP afetou positivamente o desempenho de crescimento, características histológicas dos músculos, resistência óssea e metabólitos séricos em frangos de corte.

Abstract Three hundred and twenty day old Hubbard broilers were randomly allocated to four treatments (8 replicates, 10 birds/pen) and were raised under standard management conditions. Birds in the first group served as control and were fed a corn based diet, while birds in the remaining three groups i.e.; A, B and C were fed with a basal diet supplemented with copper nanoparticles (CuNP) at 5, 10 and 15 mg /kg of diet respectively for 35 days. Supplementation of CuNP linearly increased (P≤0.05) body weight (BW), average daily weight gain (ADWG) and feed intake (FI) in broilers. Uric acid, glucose levels in blood and feed conversion ratio (FCR) reduced linearly (P≤0.05) with CuNP supplementation in diet. Supplementation of CuNP in the diet also linearly increased (P≤0.05) tibia weight, length, diameter, weight/length index (W/L) and Tibiotarsal index (TT index). Inclusion of CuNP in broilers diet linearly increased the measured parameters of muscle i.e.; pH, fiber diameter, fiber cross-sectional area, fascicle diameter, fascicle cross-sectional area (P≤0.05). Concentration of copper, iron, calcium and phosphorous in blood also increased line-arly (P ≤ 0.05) with CuNP supplementation. Overall, CuNP positively affected the growth performance, histological characteristics of muscles, bone strength and serum metabolites in broilers.

Resumen Antecedentes: Capsicum annuum var. glabriusculum es el ancestro de los chiles cultivados económicamente más importantes del mundo. En México, esta planta tiene una amplia distribución y todos los pueblos originarios vivos del país interactúan con ella. En la península de Yucatán, los Mayas conocen al chile silvestre como maax ik. Aunque esta zona ha sido reconocida como un probable centro de domesticación y diversificación del chile, la investigación etnobotánica con el maax ik, es aún limitada. Preguntas: ¿Qué conocimiento ecológico poseen las personas locales sobre el maax ik? ¿Dónde y cómo se recolecta y maneja el maax ik? ¿Qué usos actuales tiene el maax ik? Especie de estudio: Capsicum annuum var. glabriusculum. Métodos: Entrevistas semiestructuradas a profundidad y observación participante. Se identificaron las prácticas de manejo y se calculó un índice de intensidad de manejo. Resultados: Los informantes saben cómo se reproduce el maax ik y sobre el papel que las aves juegan en la dispersión de sus semillas. Maax ik fue recolectado en huertos familiares, donde fue tolerada y sujeta a otras prácticas de manejo incipientes como la protección y promoción. La recolección fue no destructiva y usualmente solo se tomaba una pequeña porción de los frutos disponibles. El maax ik fue usado esencialmente como alimento. El índice de intensidad de manejo fue relativamente alto (5). Conclusiones: Maax ik es un recurso alimenticio de subsistencia que nuestros informantes usan de forma sostenible y se manejan de forma no selectiva en los huertos familiares de San Bernardo, Yucatán.

Abstract Background: Capsicum annuum var. glabriusculum is the ancestor of most of the economically important chiles in the world. It is widely distributed throughout Mexico, and currently all indigenous groups interact with this plant species. On the Yucatan Peninsula wild chile is known as maax ik by the Maya. Although this area has been recognized as a domestication and diversification center of chile, ethnobotanical research on this species is still limited. Questions: What ecological knowledge do local people have about maax ik? Where and how is maax ik gathered and managed? What uses does maax ik currently have? Studied species: Capsicum annuum var. glabriusculum Study site and dates: San Bernardo, Yucatan. Summer-Autumn 2021. Methods: In-depth semi-structured interviews and participatory observation. The management practices were identified, and management intensity was estimated. Results: Informants knew that maax ik reproduces sexually and recognized the role of birds in its seed dispersal. Maax ik was mainly gathered from homegardens where this species was tolerated and other non-selective incipient management practices such as protection and promotion were also carried out. Fruit was harvested without harming the plant and only a small portion of the available fruit was usually taken. The informants used maax ik essentially as food. The management intensity index was relatively high (5). Conclusions: Maax ik is a sustenance food resource that is sustainably used and non-selectively managed in homegardens by our informants in San Bernardo, Yucatan.

Resumen Objetivo: Identificar la prevalencia, causas y evolución clínica de las pacientes con reintervención quirúrgica por complicaciones durante la cesárea. Material y métodos: Se revisó el archivo de la Unidad de Toco-Quirúrgica del Hospital Ginecobstetricia No. 3 del Centro Médico Nacional “La Raza” Instituto Mexicano del Seguro Social para conocer el total de pacientes sometidas a cesárea desde enero hasta diciembre de 2019 y se seleccionaron los casos con reintervención por complicaciones durante la cesárea. Se estudiaron sus datos generales, la causa de reintervención, estancia en la Unidad de Cuidados Intensivos (UCI), estancia en hospital y la mortalidad. Los datos se analizaron con estadística descriptiva utilizando el programa estadístico SPSS versión 20. Resultados: Se encontró que 3371 pacientes fueron sometidas a cesárea de las cuales 1.60% (54 casos) se reintervinieron por las siguientes causas: desempaquetamiento 27.79%, hemorragia obstétrica 20.37%, sangrado por atonía uterina 20.37%, hematoma de la comisura de histerotomía 18.52%, infiltración uterina 3.70%, lesión vascular 3.70%, lesión vesical 3.70% y lesión colónica 1.85%. La estancia en UCI fue 3.79 ± 2.03 días, estancia en hospital 13.67 ± 11.16 días y mortalidad 1.85%. Conclusión: La prevalencia de reintervención fue reducida, el sangrado fue la principal causa y la evolución clínica resultó satisfactoria con baja mortalidad.

Abstract Objective: The objective of the study is to identify the prevalence, causes, and clinical evolution of patients with surgical reintervention due to complications during cesarean section. Materials and methods: The file of the Toco-Surgical Unit of the Gynecological Obstetrics Hospital No. 3 of the National Medical Center “La Raza” Mexican Institute of Social Security was reviewed to know the total number of patients undergoing cesarean section from January to December 2019 and cases with reintervention due to complications during cesarean section were selected. Their general data, the cause of reintervention, stay in the intensive care unit (ICU), hospital stay, and mortality were studied. The data were analyzed with descriptive statistics using the statistical program SPSS version 20. Results: It was found that 3371 patients underwent cesarean section, of which 1.60% (54 cases) underwent reoperation for the following reasons: Unpacking 27.79%, obstetric hemorrhage 20.37%, bleeding due to uterine atony 20.37%, hysterotomy commissure hematoma 18.52%, uterine infiltration 3.70%, vascular injury 3.70%, bladder injury 3.70%, and colonic injury 1.85%. The ICU stay was 3.79 ± 2.03 days, hospital stay was 13.67 ± 11.16 days, and mortality was 1.85%. Conclusion: The prevalence of reintervention was reduced, bleeding was the main cause, and the clinical evolution was satisfactory with low mortality.

Resumen Los coronavirus (CoV) causan enfermedades respiratorias y entéricas leves, graves o críticas, pudiendo ocasionar la muerte del paciente. Debido a la alta conectividad entre naciones y a la transmisión, actualmente la COVID-19 representa un verdadero problema de salud pública en todo el mundo. El CoV-2 causante del síndrome respiratorio agudo grave (SARS-CoV-2) apareció a finales de diciembre de 2019 en Wuhan, China, y en marzo de 2020 la COVID-19 fue declarada pandemia. En esta revisión se describen las características del genoma y de la proteína espiga del SARS-CoV-2, su papel en la inmunopatogénesis de la COVID-19, la tormenta de citocinas, la actividad citotóxica inducida por células T y la producción de anticuerpos contra el SARS-CoV-2 mediada por células B, así como la eficacia de algunas vacunas, tomando en cuenta las mutaciones presentes en la proteína espiga.

Abstract Coronavirus (CoV) infections cause respiratory and enteric diseases with clinical manifestations ranging from faint to severe, even lead to death of patients. High connectivity between nations and infectivity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), represent a global health problem as the coronavirus disease 19 (COVID-19). This CoV-2 that cause SARS, which appeared in Wuhan, China, in December 2019 originated COVID-19 and declared as pandemic a few months posterior its appearance. In this review, the genomic and spike protein characteristics of SARS-CoV-2, the role of SARS-CoV-2 in the COVID-19 pathogenesis, cytokine storm, the role of cytotoxic T and B cells against SARS-CoV-2, as well as the vaccines efficacy (taking into account mutations in the spike protein) are described.

ABSTRACT Revision of the La Veteada Formation in its stratotype (La Rioja, Argentina): age, stratigraphy and paleoenvironmental significance. The La Veteada Formation, in the Sierra de Famatina (west of Argentina), is one of the few records of Early Triassic age in South America verified by isotopic age and palynological assemblages. This unit is composed of sandstones, mudstones, shales, limestones, and marls, with some levels of gypsum, chert, and tuff levels. In its stratotype, the La Veteada Formation rests on Late Permian red beds of the Talampaya Formation and is covered by Neogene breccias and conglomerates belonging to the Del Crestón Formation. The unit is divided into three associations of sedimentary facies. Facies association A (80 m thick) is a red bed succession composed of mudstone and fine-grained sandstone, together with some levels of chert and gypsum. Facies association B (58 m thick) is greenish-gray to yellowish-gray and comprises limestones, marls, shales, and fine- to coarse-grained sandstones. This facies includes stromatolites and several levels of shales and marls where rich palynological assemblages were recovered. Finally, facies association C (24 m thick) is made up of yellowish-gray marls, mudstones, and sandstones. In this section, stromatolites are missing, thin levels of gypsum appear, and evidence of subaerial exposition as mud cracks and raindrop imprints are frequent at the top of the facies. The lithology, sedimentary structures, and vertical stacking of facies suggest that the La Veteada Formation was deposited in a lacustrine environment, which evolved from a shallow ephemeral playa lake system (facies association A) to a perennial lake, in which carbonate production increased compared to clastic sedimentation (facies association B). Regarding the facies association C, the increase in clastic supply, the missing stromatolites, and the presence of gypsum levels suggest a progressive shallowing of the lake and the likely transition to a palustrine environment. The Early Triassic age of the La Veteada Formation is indicated from two different lines of research. Firstly, a zircon U-Pb age of 249.66±0.11 Ma obtained from a tuff level at the middle part of the facies association A. Secondly, the presence of palynological species identified in other Early Triassic units worldwide. The La Veteada Formation records the filling of the embryonic extensional basins formed at the earliest Triassic. Moreover, this unit shows the evolution of depositional environments after the late Permian massive extinction event.

RESUMEN La Formación La Veteada, en la sierra de Famatina (oeste de Argentina), es uno de los pocos registros de edad triásica temprana en América del Sur comprobada por dataciones radiométricas y asociaciones palinológicas. Esta unidad está compuesta por areniscas, lutitas, fangolitas, calizas y margas, con algunas intercalaciones de yeso, chert y toba. En su estratotipo, descansa sobre bancos rojos de la Formación Talampaya (Pérmico tardío) y está cubierta por brechas y conglomerados del Neógeno pertenecientes a la Formación Del Crestón. La unidad es dividida en tres asociaciones de facies sedimentarias. La asociación de facies A (80 m de espesor) es una sucesión de capas rojas compuestas por fangolitas y areniscas de grano fino, junto con algunos niveles de chert y yeso. La asociación de facies B (58 m de espesor) es de color gris verdoso a gris amarillento y comprende calizas, margas, lutitas y areniscas de grano fino a grueso. Esta facies incluye estromatolitos y varios niveles de lutitas y margas donde se recuperaron ricas asociaciones palinológicas. Finalmente, la asociación de facies C (24 m de espesor) está compuesta por margas, lutitas y areniscas de color gris amarillento. En esta sección, faltan estromatolitos, aparecen niveles delgados de yeso y son frecuentes las evidencias de exposición subaérea, como grietas de desecación e impresiones de gotas de lluvia, especialmente en la parte superior de la facies. La litología, las estructuras sedimentarias y el apilamiento vertical de facies sugieren que la Formación La Veteada se depositó en un ambiente lacustre, que evolucionó de un sistema lacustre efímero poco profundo (asociación de facies A) a un lago perenne, en el que la producción de carbonato aumentó en comparación con la sedimentación clástica (asociación de facies B). En la asociación de facies C, el aumento en el suministro clástico, la ausencia de estromatolitos y la presencia de niveles de yeso indicarían una progresiva somerización del lago y la probable transición a un ambiente palustre. La edad triásica temprana de la Formación La Veteada es determinada desde dos líneas de investigación diferentes. En primer lugar, una edad U-Pb sobre circón de 249,66±0,11 Ma obtenida a partir de un nivel de toba en la parte media de la asociación de facies A. En segundo lugar, la presencia de especies palinológicas identificadas en otras unidades del Triásico temprano a nivel mundial. La unidad registra el relleno de las embrionarias cuencas extensionales formadas a comienzos del Triásico. Por otro lado, la Formación La Veteada muestra la evolución de los ambientes depositacionales con posterioridad al evento de extinción masiva ocurrido a fines del Pérmico.

Resumen Introducción: Los “jardines de hormigas'' son nidos de hormigas en árboles, que se localizan a diferentes alturas, sobre los que germinan plantas epífitas vasculares que han sido transportadas y sembradas previamente por hormigas. Aunque esta relación mutualista ha sido estudiada en ecosistemas húmedos tropicales, la información en otros ecosistemas tropicales y en Colombia, es escasa. Objetivo: Caracterizar la distribución, construcción, diversidad, demografía y fenología de los hormigueros en bosque tropical seco. Métodos: En enero y febrero de 2018, identificamos 170 hormigueros en un transecto de 100x5 m a orillas del río Quesada, Colombia, y en el bosque secundario premontano seco de transición adyacente; monitoreamos cambios, para jardines y plantas, en 28 de ellos, cada dos semanas (marzo 2018-febrero 2019). Resultados: Los jardines, construidos por Azteca ulei, se agregaron cerca de cuerpos de agua; tenían 10 especies de epífitas y estaban sobre 13 especies arbóreas. Los jardines más grandes tenían más especies epífitas. Algunas epífitas tuvieron un patrón fenológico bimodal. Se establecen menos plántulas en la estación seca y quedan menos adultos en los jardines. Las características del jardín benefician tanto a las epífitas como a las hormigas. Conclusiones: Los jardines construidos por A. ulei tienen características ecológicas que favorecen la germinación, establecimiento y reproducción de diversas epífitas en este ecosistema tropical seco, incluyendo la agregación cerca de cursos de agua.

Abstract Introduction: “Ant gardens'' are ant nests located at different heights on trees on which vascular epiphytic plants that have been transported and sown by ants have germinated. Although this mutualistic relationship has been studied in humid tropical ecosystems, information on other tropical and Colombian ecosystems is scarce. Objective: To characterize the distribution, building, diversity, demography and phenology of ant gardens in dry tropical forest. Methods: In January and February, 2018, we identified 170 ant gardens on a 100x5 m transect on the banks of the Quesada River, Colombia, and in adjacent secondary dry premontane transitional forest; we monitored changes, for gardens and plants, in 28 of them, every two weeks (March 2018-February 2019). Results: The gardens, built by Azteca ulei, were aggregated near water bodies; had 10 species of epiphytes and were on 13 tree species. Larger gardens had more epiphyte species. Some epiphytes had a bimodal phenological pattern. Less seedlings become established in the dry season, and less adults remain in the gardens. Garden characteristics benefit both epiphytes and ants. Conclusions: The gardens built by A. ulei have ecological characteristics that favor the germination, establishment, and reproduction of diverse epiphytes in this dry tropical ecosystem, including aggregation near water flows.

Abstract: This research study analyzes the relationship between transformational leadership (TL), job satisfaction (JS), and turnover intention (TI) among public primary education teachers from the Antofagasta region (Chile). A questionnaire is structured based on instruments validated on international literature. Teachers are selected through a non-probabilistic convenience sampling. Data analyses are performed using the two-stage method in structural equations. The results indicate that the proposed model is able to explain job satisfaction and turnover intention. The results show a negative and significant association between age and turnover intention. It is concluded that transformational leadership style plays an important role in job satisfaction, which affects teacher’s turnover intention.

Resumen: Esta investigación analiza la relación entre el liderazgo transformacional (LT), la satisfacción laboral (SL) y la intención de dejar el trabajo (IDT), en el contexto de los docentes de educación primaria pública de la región de Antofagasta (Chile). Se estructura un cuestionario en base a los instrumentos validados en la literatura internacional. Los docentes son seleccionados a través de un muestreo no probabilístico por conveniencia. El análisis de los datos se realiza mediante el método de dos etapas en ecuaciones estructurales. Los resultados señalan que el modelo planteado tiene una significativa e importante capacidad de explicar la satisfacción laboral y la intención de dejar el trabajo. Los resultados muestran una asociación negativa y significativa entre la edad y la intención de dejar el trabajo. Se concluye que el estilo de liderazgo transformacional juega un importante rol en la satisfacción laboral, y a su vez esta última en la intención de dejar el trabajo por parte de los docentes.

Resumen La presente investigación tuvo como objetivos analizar la relación entre creatividad y autoconcepto académico, así como identificar diferencias por sexo de las variables de estudio en niños de tercero a sexto grado de primaria. La muestra fue no probabilística por conveniencia, conformada por 269 estudiantes de una escuela pública ubicada en Nezahualcóyotl, Estado de México. Todos fueron evaluados con la escala de autoconcepto académico y la prueba de pensamiento creativo figural A. Al analizar los resultados, se encontró una asociación positiva y significativa entre el autoconcepto académico y la creatividad (r = 0.2**, p < 0.01), y dos correlaciones negativas del autoconcepto académico, una con el indicador de títulos (r = -0.2**, p < 0.01) y otra con el de cierre (r = -0.3**, p < 0.01). En cuanto a la variable sexo, con la prueba t de Student se encontró que las niñas mostraron puntajes más altos de creatividad en comparación con los niños (t = -3.008, p = 0.00). Se concluye que la percepción de los niños, respecto a su capacidad como estudiantes, representa un elemento importante para el desarrollo del pensamiento creativo.

Abstract This research aimed to analyze the relationship between creativity and academic self-concept as well as to identify differences by sex for study variables among 3rd to 6th grade students. A non-probabilistic convenience sample included 269 public school students located in Nezahualcóyotl, Estado de México. The study administered the Academic Self-concept Scale and the Figural version of the Torrance Test of Creative Thinking. Results showed a statistically significant correlation between academic self-concept levels and creativity skills such as originality, title, and closure. Regarding sex differences, a t test found higher scores in creativity for girls than boys (t= -3.008, p= 0.003). It is concluded that the perception children have of their own abilities as students represents an important element for the development of creative thinking.

Resumen Introducción. Desde el primer reporte en la provincia de Wuhan (China) en el año 2019, el SARS-CoV-2 se ha diseminado por todo el mundo, provocando un enorme impacto en la salud pública. Para su diagnóstico, la Organización Mundial de la Salud ha incentivado el desarrollo de pruebas rápidas, de simple ejecución, sensibles y específicas, que complementan la RT-qPCR como prueba de referencia. La prueba RT-LAMP ha mostrado ser una excelente alternativa para la detección del SARS-CoV-2 en diferentes biofluidos. Objetivo. Validar la técnica RT-LAMP colorimétrica en muestras de hisopado nasofaríngeo previamente confirmadas por RT-qPCR, usando el protocolo Charité, Berlín, Alemania. Materiales y métodos. Un total de 153 muestras de hisopado nasofaríngeo de individuos con sospecha de COVID-19 se sometieron a RT-qPCR y RT-LAMP, usando un estuche comercial colorimétrico (NEB, Germany). La RT-LAMP se practicó con las muestras de ARN extraídas del hisopado nasofaríngeo y con muestras crudas sin previa extracción de ARN. El resultado fue evaluado por un simple cambio de color en la reacción. Resultados. La sensibilidad y especificidad de la técnica RT-LAMP para detectar el gen N del SARS-CoV-2 mediante un set de cebadores previamente reportados (set de Broughton), arrojó valores de 0,97 (0,85-1,00) y 0,81 (0,65-0,92), respectivamente, con un intervalo de confianza del 95%. Otro set de cebadores dirigidos contra otra región del mismo gen (set de Lalli) arrojó valores de sensibilidad y especificidad de 0,96 (0,78-1,00) y 0,77 (0,55-0,92), respectivamente. Sin previa extracción de ARN, se encontró que la sensibilidad fue del 0,95 (0,74-1,00) y la especificidad del 0,88 (0,64-0,99). Conclusiones. Estos resultados evidencian que la técnica RT-LAMP podría considerarse una prueba diagnóstica rápida, de fácil ejecución, libre de equipos sofisticados, sensible y específica, para el diagnóstico del SARS-CoV-2 en muestras de hisopados nasofaríngeos.

Abstract Introduction: Since the first report in Wuhan (China) in 2019, the SARS-CoV-2 virus has spread throughout the world, with a significant impact in public health. To contain its transmission, the WHO has encouraged the development of rapid, simple, sensitive and specific tests that complement qRT-PCR, as the gold standard. RT-LAMP has shown to be a good alternative to detect SARS-CoV-2 in different fluid samples. Objective: To validate the colorimetric RT-LAMP technique using two sets of primers targeting N gene of SARS-CoV-2 in 117 nasopharyngeal swab samples previously confirmed by RT-qPCR, using the Charité/Berlin protocol. Material and methods: A total of 153 nasopharyngeal swab samples from individuals with suspected COVID-19 were subjected to qRT-PCR and RT-LAMP using a commercial colorimetric kit (NEB, Germany). RT-LAMP was performed using both extracted RNA samples and raw samples without prior RNA extraction, and the result was assessed by a simple color change in the reaction. Results: Sensitivity and specificity for the previously reported RT-LAMP primers (Broughton set) targeting N gene of SARS-CoV-2 were 0.97 (0.85-1.00) and 0.81 (0.65-0.92) respectively, with CI95%. The Lalli primers targeting another region of the N gene used showed a sensitivity value of 0.96 (0.78-1.00) and a specificity of 0.77 (0.55-0.92). Without RNA extraction we found a sensitivity value of 0.95 (0.74, 1.00) and a specificity of 0.88 (0.64, 0.99). A sensitivity value of 0.95 (0.74-1.00) and a specificity 0.88 (0.64-0.99) were found without prior RNA extraction. Conclusion: Taking together, the results showed that RT-LAMP technique could be considered as a rapid diagnostic test, easy to perform, free of sophisticated equipment, sensitive and specific to diagnose SARS-CoV-2 in nasopharyngeal swabs with and without prior RNA extraction, allowing its implementation in places with scarce resources.

ABSTRACT Background: Insulin resistance is key in the pathogenesis of the metabolic syndrome and cardiovascular disease. Objective: We aimed to identify glucose and insulin patterns after a 5-h oral glucose tolerance test (OGTT) in individuals without diabetes and to explore cardiometabolic risk factors, beta-cell function, and insulin sensitivity in each pattern. Methods: We analyzed the 5-h OGTT in a tertiary healthcare center. We identified classes using latent class trajectory analysis and evaluated their association with cardiometabolic risk factors, beta-cell function, and insulin sensitivity surrogates by multinomial logistic regression analysis. Results: We included 1088 5-h OGTT performed between 2013 and 2020 and identified four classes. Class one was associated with normal insulin sensitivity and secretion. Class two showed hyperglycemia, dysinsulinism, and a high-risk cardiometabolic profile (obesity, hypertriglyceridemia, and low high-density lipoprotein [HDL] cholesterol). Class three included older individuals, a higher proportion of males, and a greater prevalence of hypertension, hyperglycemia, hyperinsulinemia, and postprandial hypoglycemia. Finally, class four showed hyperglycemia, dysinsulinism, and hyperinsulinemia; this class had the worst cardiometabolic profile (a high proportion of males, greater age, hypertension, obesity, hypertriglyceridemia, and low HDL cholesterol, p < 0.001 vs. other classes). Conclusions: The latent class analysis approach allows the identification of groups with an adverse cardiometabolic risk factor, and who might benefit from frequent follow-ups and timely multidisciplinary interventions.

Abstract Crotalid envenomation is a neglected collective health problem involving many countries in America, which need secure and inexpensive snake anti-venom treatments. Here, high antibody titers (IgY) were raised in the Ostrich (Struthio camelus) egg yolk by immunizing with the venom of Venezuelan venomous Crotalus snakes. Ostriches were immunized with a pool of venoms from common rattlesnake (Crotalus durissus cumanensis), Uracoan rattlesnake (Crotalus vegrandis), Guayana rattlesnake (Crotalus durissus ruruima) and black rattlesnake (Crotalus pifanorum). The anti-snake venom antibodies were prepared from egg yolk by the water dilution method, enriched by the addition of caprylic acid (CA) and precipitation with ammonium sulfate at 30% (W/V). The purity and molecular mass of the final product was satisfactory, yielding a single ∼ 175 kDa band in SDS-PAGE gels ran under non-reducing conditions. In the immunoblot analysis, specific binding of the antivenom was observed with most venom proteins. The LD50 was 16.5 g/mouse (825 μg/kg body weight). High titers of IgY against Crot/pool venom were shown by ELISA. The median effective dose (ED50) was 19.66 mg/2LD50. IgY antibodies neutralized efficiently the Crot/pool venom lethality. As far as we know, this is the first anti-snake venom produced in ostriches, which could make this technology an affordable alternative for low-income countries, since it is likely to produce manteniabout 2-4 g of IgY per ostrich egg. Hence, almost 400 g of IgY can be purified from only one ostrich during a year. In addition, there are enormous differences in the cost of investment in the maintenance of horses, from the points of view of infrastructure, feeding and veterinary care, in which the cost can reach USD 100 per animal per day, compared to a maintenance cost of USD 146 per month per producing bird. These results are encouraging and could easily be extrapolated to the manufacturing of other antivenoms and antitoxins as well, as they could be applied to the manufacturing of potential diagnostic tools.

Resumen El envenenamiento por crotálidos es un problema de salud colectiva desatendido, que involucra a muchos países del continente americano, los cuales necesitan tratamientos seguros y económicos. En este trabajo, se obtuvieron títulos altos de anticuerpos (IgY) producidos en yema de huevo de avestruz (Struthio camelus) mediante la inmunización con el veneno de serpientes venezolanas del genero Crotalus. Se inmunizaron avestruces con una colección de veneno de serpientes de cascabel común (Crotalus durissus cumanensis), cascabel de Uracoa (Crotalus vegrandis), cascabel de Guayana (Crotalus durissus ruruima) y cascabel negra (Crotalus pifanorum). Los anticuerpos anti-veneno de serpiente se prepararon a partir de yema de huevo por el método de dilución en agua, enriquecidos mediante la adición de ácido caprílico (CA), seguido de una precipitación con sulfato de amonio al 30% (P/V). La pureza y masa molecular de los anticuerpos (IgY) se definieron mediante ensayos de SDS-PAGE nativos y las masas moleculares se establecieron electroforéticamente, obteniéndose una única banda de IgY de ∼ 175 kDa. El análisis de inmunotransferencia mostró la unión específica del antiveneno con la mayoría de las proteínas del veneno. La DL50 fue de 16,5 μg/ratón (825 μg / kg de peso corporal); Se mostraron títulos altos de IgY contra el veneno de Crot / pool mediante ELISA. La dosis mediana efectiva (DE50) fue de 19,66 mg/2 LD50. Los anticuerpos IgY neutralizaron eficazmente la letalidad del veneno de Crot / pool. Hasta donde sabemos, se trata del primer antídoto de serpiente producido en avestruces, lo que podría abaratar la producción de este tratamiento en países del tercer mundo. Ya que es probable que se obtengan alrededor de 2-4 g de IgY por huevo de avestruz. Por lo tanto, se podrían purificar casi 400 g de IgY de un solo avestruz durante un año. Asimismo, debido a las enormes diferencias en el costo de inversión en el mantenimiento de los caballos desde el punto de vista de infraestructura, alimentación y atención veterinaria, en los que el costo puede llegar a los 100 USD por día, frente a los 146 USD por mes de mantenimiento de la producción de aves. Estos resultados abren un campo terapéutico, para la fabricación de otros antivenenos contra un amplio espectro de toxinas y también como probables herramientas de diagnóstico.

RESUMO Objetivo: Este estudo descreve as razões de permanência e de potencial saída voluntária de profissionais de organizações de tecnologia da informação (TI) de Santa Catarina. Originalidade/valor: Organizações de TI têm vivenciado desafios para reter profissionais. Diante dessa realidade, este estudo contribui para a literatura sobre o tema ao apresentar a percepção de profissionais sobre as suas razões para permanecerem nessas organizações ou saírem voluntariamente delas. Design/metodologia/abordagem: Quatrocentos e quarenta trabalhadores de empresas privadas de TI responderam livremente a duas perguntas abertas em um questionário on-line (survey): “Por que você permanece trabalhando nesta empresa?” e “Por que você poderia deixar esta em - presa no futuro?”. As respostas, coletadas no segundo semestre de 2016, foram codificadas indutivamente e depois agregadas em categorias. Resultados: Os resultados indicam que os profissionais permanecem nas organizações pesquisadas prioritariamente devido a razões ocupacionais (satisfação no trabalho, comprometimento organizacional, condições de trabalho adequadas e percepção de crescimento profissional), organizacionais (práticas de gestão adequadas) e ambientais (percepção de que não há melhores alternativas de trabalho). Os profissionais pesquisados deixariam as organizações em que trabalham também prioritariamente por razões ocupacionais (ausência de satisfação no trabalho e de oportunidades para crescimento profissional, falta de perspectivas de valorização e por melhores condições de trabalho), bem como por razões organizacionais (práticas de gestão inadequadas), ambientais (melhores alternativas de trabalho) e individuais (planejamento de carreira). A partir da análise desses resultados, é apresentada uma agenda para pesquisas futuras sobre o tema investigado.

ABSTRACT Purpose: This study describes the reasons for professionals to stay or leave information technology (IT) organizations in Santa Catarina. Originality/value: Technology organizations have experienced challenges in retaining professionals. Therefore, this study contributes to the literature on the subject by presenting the professionals’ perceptions about their reasons for voluntarily staying or leaving these organizations. Design/methodology/approach: Four hundred and forty workers from private technology companies freely answered two open questions in an online questionnaire (survey): “What are the reasons that maintain you working at this company?” and “For what reasons would you leave this company in the future?”. The responses, collected in the second half of 2016, were inductively coded and subsequently aggregated into categories. Findings: The results indicate that the professionals remain in the researched organizations primarily due to occupational (job satisfaction, organizational commitment, proper working conditions and perception of professional growth), organizational (adequate management practices) and environmental reasons (perception that there are no better work alternatives). The professionals surveyed would leave the organizations they currently work, primarily for occupational reasons (lack of job satisfaction and opportunities for professional growth, lack of an outlook on valorization and better working conditions), as well as for organizational (inadequate management practices), environmental (better job alternatives) and individual reasons (career planning). Based on the analysis of these results, an agenda for future research on the investigated topic is presented.

RESUMO Objetivo: Comparar as diferenças entre as abordagens endoscópicas transforaminal e interlaminar quanto à intensidade da dor e a funcionalidade 30 dias depois do procedimento cirúrgico. Métodos: Estudo de coorte retrospectivo, com pacientes tratados por discectomia endoscópica percutânea interlaminar ou transforaminal, em acompanhamento no Serviço de Coluna da ISCMPA, sul do Brasil. Os dados foram coletados do prontuário eletrônico dos pacientes por dois médicos independentes. Os desfechos clínicos de intensidade de dor e funcionalidade lombar foram avaliados, respectivamente, pela pontuação da escala visual analógica e do Oswestry Disability Index. Resultados: Trinta e seis pacientes foram incluídos no estudo; a média de idade foi 50,8 ± 15,3 anos, sendo 19 (52,8%), do sexo masculino. Quanto aos desfechos clínicos, observou-se diferença estatisticamente significativa para as abordagens endoscópicas percutânea transforaminal e interlaminar na redução da intensidade da dor (média da diferença 3,5 pontos, p < 0,001) e na melhora da funcionalidade (média da diferença 33,2 pontos, p < 0,001) quando comparados os momentos pré e em 30 dias pós-operatórios, sem diferença significativa com relação às abordagens. O tipo de abordagem técnica diferiu também com relação à idade dos pacientes, à localização, ao tipo e à migração da hérnia de disco e ao tempo do paciente em sala cirúrgica. Conclusões: Observou-se efeito semelhante na redução da dor e na restauração das funções lombares, 30 dias depois da discectomia endoscópica percutânea, em ambas as abordagens técnicas, sem diferenças significativas entre si. Nível de Evidência III;Retrospectivo comparativo.

ABSTRACT Objective: To compare the differences between transforaminal and interlaminar endoscopic approaches in terms of pain intensity and functionality 30 days after the surgical procedure. Methods: A retrospective cohort study, with patients treated by percutaneous interlaminar or transforaminal endoscopic discectomy at the Spine Service of the ISCMPA, in southern Brazil. Data were collected from the patients’ electronic medical records by two independent physicians. The clinical outcomes of pain intensity and lumbar functionality were evaluated, respectively, using the visual analogue scale and the Oswestry Disability Index. Results: Thirty-six patients were included in the study, with a mean age of 50.8 ± 15.3 years, 19 (52.8%) of whom were males. As for the clinical outcomes for both transforaminal and interlaminar percutaneous endoscopic approaches, we observed a statistically significant reduction in pain intensity (mean difference of 3.5 points, p < 0.001) and a statistically significant improvement in functionality (mean difference of 33.2 points, p < 0.001) when we compared the pre- and 30-day postoperative periods, with no significant differences in terms of approaches. The type of technical approach also differed in relation to the patients’ age, the location, type, and migration of the herniated disc, and the patient’s time in the operating room. Conclusion: There was a similar effect on pain reduction and restoration of lumbar functions, 30 days after percutaneous endoscopic discectomy, in both technical approaches, with no significant differences between them. Level of Evidence III; Retrospective comparative study.

RESUMEN Objetivos: Comparar las diferencias entre los abordajes endoscópicos transforaminal e interlaminar en cuanto a la intensidad y funcionalidad del dolor a los 30 días del procedimiento quirúrgico. Métodos: Estudio de cohorte retrospectivo, con pacientes tratados por discectomía endoscópica percutánea interlaminar o transforaminal, en acompañamiento en el Servicio de Columna – ISCMPA, sur de Brasil. Los datos fueron recopilados de las historias clínicas electrónicas de los pacientes por dos médicos independientes. Los resultados clínicos de la intensidad del dolor y la funcionalidad lumbar se evaluaron, respectivamente, utilizando la escala visual analógica y el Oswestry Disability Index. Resultados: Se incluyeron en el estudio 36 pacientes, con una edad promedio de 50,8 ± 15,3 años, 19 (52,8%) varones. En cuanto a los resultados clínicos, se observó una diferencia estadísticamente significativa para los enfoques endoscópicos percutáneos transforaminal e interlaminar en la reducción de la intensidad del dolor (diferencia media 3,5 puntos, p <0,001) y en la mejora de la funcionalidad (diferencia media 33,2 puntos, p <0,001) al comparar los períodos preoperatorios y postoperatorios a los 30 días, sin diferencia significativa en cuanto a los enfoques. El tipo de enfoque técnico también difirió con respecto a la edad de los pacientes, la ubicación, el tipo y la migración de la hernia de disco, y el tiempo del paciente en el quirófano. Conclusiones: Hubo un efecto similar en la reducción del dolor y la restauración de las funciones lumbares, 30 días después de la discectomía endoscópica percutánea, en ambos enfoques técnicos, sin diferencias significativas entre ellos. Nivel de Evidencia III; Estudio retrospectivo comparativo.

Abstract Introduction: Anhedonia is a critical symptom of major depressive disorder that is defined as the reduced ability to experience pleasure. The Temporal Experience of Pleasure Scale (TEPS) is commonly used to measure anhedonia and has exhibited satisfactory reliability. Objectives: We aim to perform cross-cultural adaptation of a Brazilian version of the TEPS and evaluate its psychometric properties. Method: The cross-cultural adaptation was performed according to previously established protocols. Cronbach’s alpha coefficient of internal consistency was used to establish the degree of interrelation and coherence of items. Also, we calculated the intraclass correlation coefficient to determine the stability of the scale after a proposed interval had elapsed and used exploratory factor analysis to evaluate the scale’s factor structure and content validity. Principal component analysis was used to determine the factors to be retained in the factor model. Results: The participants reported that the Brazilian version of the TEPS had good comprehensibility and applicability. The results revealed a statistically significant correlation between measures. The intraclass correlation coefficient calculated was significant. The Cronbach’s alpha value calculated indicated that the scale’s overall internal consistency was adequate. Conclusion: The Portuguese version of the TEPS scale proposed achieved good comprehensibility for the Brazilian population and its psychometric characteristics demonstrated good reliability and validity.