Abstract Roadkill is one of the most direct impacts on fauna, leading to numerous direct and indirect impacts. Road-killed animals could serve as a resource for research and teaching purposes, but first, they need to have their tissues preserved by some technique. Plastination is one of the most modern techniques for the conservation of biological tissues, its principle is the replacement of body fluids by a curable polymer, resulting in a non-toxic, odorless, and manipulable material. So the main aim of this work was to compose a collection of plastinated roadkill victims from the Biological Reserve Sooretama region, adapting the standard protocol to the different taxa worked here (Avialae, Reptilia, Amphibia, and Mammalia). For this, 42 animals were used, previously frozen and stored in the Sooretama Biological Reserve and Vale Natural Reserve until they were sent for processing. Upon arriving at the Plastination laboratory at the Federal University of Espírito Santo, the animals underwent the 4 stages of plastination: fixation, dehydration, forced impregnation, and chemical cure; some adaptations were made to improve the final specimens. The results proved that the plastination of these animals is extremely viable in generating satisfactory results. Given the results obtained, it becomes clear how plastination can become an alternative to implement in the composition of biological collections using roadkill animals. The animals prepared in this work comprise an unprecedented collection available for different applications, such as exhibitions, practical classes, and research.

ABSTRACT Background: Domestic pigeons carry pathogens in their droppings, posing a potential public health problem. Methods: The phenotypic and genotypic antimicrobial resistances of Staphylococcus aureus and Enterococcus faecium in the feces of urban pigeons near hospitals with intensive care units were measured. Results: Twenty-nine samples showed Enterococcus growth, whereas one was positive for S. aureus. The S. aureus isolate was sensitive to the antibiotics tested via antibiogram, however resistance genes were identified. E. faecium isolates showed phenotypic resistance to gentamicin, erythromycin, and ciprofloxacin. Conclusions: Antimicrobial profiles harmful to health were demonstrated in bacterial pathogens isolated from the external environment of hospitals.

Resumo Os sintomas da deficiência de magnésio (Mg) têm sido bem documentados em plantas cultivadas. A relação entre a deficiência de Mg, como um importante fator de estresse abiótico, e a partição do açúcares, pode restringir o crescimento das raízes e limitar o sucesso do plantio no campo. Apesar disso, os efeitos fisiológicos primários da baixa disponibilidade de Mg permanecem amplamente desconhecidos no eucalipto. Este trabalho buscou investigar como a deficiência de Mg afeta aspectos bioquímicos da partição de açúcares, associados ao acúmulo de matéria seca em raízes de plantas jovens de Eucalyptus urophylla. O experimento foi conduzido em casa de vegetação em delineamento inteiramente casualizado, com parcelas subdivididas 5 x 4, utilizando os seguintes níveis de Mg: 0, 25, 50, 75 e 100% na concentração da solução nutritiva de Clark. As avaliações foram realizadas aos 15, 30, 50 e 120 dias após o plantio (DAP) das mudas em vasos. Determinaram-se os teores de açúcares solúveis (SS) e redutores (RS), as atividades da invertase e da sacarose sintase (susy), e a matéria seca da parte aérea e da raiz. Aumentos nas concentrações de açúcares, tanto SS como RS, foram encontrados em tecidos foliares a partir de 30 DAP. Nos tecidos de raízes, os teores de RS e SS não apresentaram diferenças entre os tratamentos com deficiência de Mg e o controle. Diferenças significativas também não foram encontradas, tanto no acúmulo de matéria seca da raiz, como na razão matéria seca de parte aérea / raízes. A deficiência de Mg não afetou a clivagem de sacarose nas raízes, que foi predominantemente catalisada pela invertase ácida, seguida de susy e invertase neutra. Concluiu-se que Eucalyptus urophylla é tolerante à deficiência de Mg, uma vez que o acúmulo de açúcares nos tecidos foliares não foi suficiente para restringir o acúmulo de matéria seca nas raízes.

Abstract The symptoms of magnesium (Mg) deficiency have been well documented in crop plants. The relationship between Mg deficiency as an important abiotic stress factor and the sugar partitioning may restrict the root growth and limit the success of planting in the field. Despite of this, the primary physiological effects of low Mg availability remain largely unknown in eucalyptus. This paper aimed to investigate how the Mg deficiency affects biochemical aspects of sugar partitioning associated to dry matter accumulation in roots of Eucalyptus urophylla young plants, clone AEC 144. Experimental work was carried out in a greenhouse, arranged by completely randomized design, consisted by split plot 5x4, using the following Mg levels: 0, 25, 50, 75 and 100% in the Mg concentration of Clark’s nutrient solution. Evaluations were performed at 15, 30, 50 and 120 days after seedlings planting (DAP) in pots. Soluble (SS) and reducing (RS) sugar contents, invertase and sucrose synthase (susy) activity and shoot and root dry matter were measured. Increased sugar concentrations, both SS and RS, were found in leaf tissues from 30 DAP. In root tissues, neither RS nor SS content showed differences between Mg deficiency treatments and control. Significant differences were also not found in both root dry matter accumulation and shoot/root dry matter ratio. The Mg deficiency did not affect sucrose cleaving in roots, which was predominantly catalyzed by acidic invertase, followed by susy and neutral invertase. We concluded that Eucalyptus urophylla is tolerant to Mg deficiency, because the sugar accumulation in the leaf tissues was not enough to constrain the dry matter accumulation in roots.

Resumo O tratamento da aids implica a utilização de diversos medicamentos que compõem a terapia antirretroviral, sendo o controle logístico monitorado por um sistema nacional informatizado de dispensação, o Sistema de Controle Logístico de Medicamentos (SICLOM). O objetivo foi investigar a utilização da terapia antirretroviral de pacientes de dois hospitais universitários no Estado do Rio de Janeiro a partir do SICLOM. Foi realizado um estudo seccional com coleta de dados sociodemográficos e de dispensação, a partir do SICLOM. A avaliação da posse para estimativa de pacientes não aderidos foi realizada através do indicador de proporção de dias cobertos (PDC). Foram incluídos 538 pacientes com cadastro ativo no SICLOM, maiores de 18 anos de ambos os sexos. O ARV mais utilizado em ambos os hospitais foi lamivudina, dentre 58 esquemas diferentes identificados. A maior parte dos pacientes utilizava esquemas classificados como de primeira linha de tratamento. Em média, a proporção de dias cobertos (posse) pelo tratamento foi 88% (± 0,16). A linha de tratamento mostrou associação estatística com a posse, sendo observado melhor PDC 91% (p < 0,001) para os esquemas resgate. Este estudo corroborou o SICLOM como uma boa fonte para conhecimento do perfil de utilização de ARV.

Abstract Treatment of AIDS involves the use of the cocktail of drugs that make up the antiretroviral therapy. Its logistic control is monitored by a computerized national system of dispensation, the Logistic Control System of Medication (SICLOM). This study aimed to investigate, by means of SICLOM data, the use of antiretroviral therapy in patients treated at two University Hospitals in the state of Rio de Janeiro. A cross-sectional study was conducted with sociodemographic and dispensation data collected from SICLOM. The evaluation of drug ownership was done by calculating the Proportion of Days Covered (PDC). Five hundred and thirty-eight patients of both genders with active registration in SICLOM and over 18 years of age were included. The ART most used in both hospitals was lamivudine, considering the total of 58 different schemes identified. The mean of possession of groups was 88% (± 0,16). The factor associated with possession of drugs was the ART scheme, with PDC of 91% (p<0,001) for rescue schemes. This study confirmed that SICLOM was a reliable source to establish the profile of the population assisted.

Resumo O tratamento da aids implica a utilização de diversos medicamentos que compõem a terapia antirretroviral, sendo o controle logístico monitorado por um sistema nacional informatizado de dispensação, o Sistema de Controle Logístico de Medicamentos (SICLOM). O objetivo foi investigar a utilização da terapia antirretroviral de pacientes de dois hospitais universitários no Estado do Rio de Janeiro a partir do SICLOM. Foi realizado um estudo seccional com coleta de dados sociodemográficos e de dispensação, a partir do SICLOM. A avaliação da posse para estimativa de pacientes não aderidos foi realizada através do indicador de proporção de dias cobertos (PDC). Foram incluídos 538 pacientes com cadastro ativo no SICLOM, maiores de 18 anos de ambos os sexos. O ARV mais utilizado em ambos os hospitais foi lamivudina, dentre 58 esquemas diferentes identificados. A maior parte dos pacientes utilizava esquemas classificados como de primeira linha de tratamento. Em média, a proporção de dias cobertos (posse) pelo tratamento foi 88% (± 0,16). A linha de tratamento mostrou associação estatística com a posse, sendo observado melhor PDC 91% (p < 0,001) para os esquemas resgate. Este estudo corroborou o SICLOM como uma boa fonte para conhecimento do perfil de utilização de ARV.

Abstract Treatment of AIDS involves the use of the cocktail of drugs that make up the antiretroviral therapy. Its logistic control is monitored by a computerized national system of dispensation, the Logistic Control System of Medication (SICLOM). This study aimed to investigate, by means of SICLOM data, the use of antiretroviral therapy in patients treated at two University Hospitals in the state of Rio de Janeiro. A cross-sectional study was conducted with sociodemographic and dispensation data collected from SICLOM. The evaluation of drug ownership was done by calculating the Proportion of Days Covered (PDC). Five hundred and thirty-eight patients of both genders with active registration in SICLOM and over 18 years of age were included. The ART most used in both hospitals was lamivudine, considering the total of 58 different schemes identified. The mean of possession of groups was 88% (± 0,16). The factor associated with possession of drugs was the ART scheme, with PDC of 91% (p<0,001) for rescue schemes. This study confirmed that SICLOM was a reliable source to establish the profile of the population assisted.