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Abstract The homemade ice cream formulations produced from the partial replacement of dairy fat with unripe banana biomass (UBB) were elaborated and characterized. Formulations 1, 2 and 3 with 2.6%, 12.5% and 21.9% of reconstituted UBB, respectively in partial replacement of heavy cream were compared to the traditional formulation (0% UBB and 100% of heavy cream). The characterization included: physicochemical, microbiological, and sensory analyses. Fat reduction of at least 22.6% in centesimal composition were noticed among the formulation with UBB (p < 0.05). The titratable acidity and meltdown rate of the formulations increased with the addition of UBB and fat reduction. The sensory analysis showed that the preference of the panellists reduced with substitution of cream for UBB. The traditional formulation and formulation 1 were the most preferred. There was no difference in the attributes of overall impression, appearance, creamy texture, color, sweet taste and vanilla flavor between the traditional formulation and formulation 1 in the acceptance test. It was also found that the intention to consume those same formulations was positive. Therefore, the production of ice cream using prebiotic fat replacers are an interesting option to be explored in an attempt to combine sensory acceptance with health benefits.

https://doi.org/10.1590/fst.41722

Intra-abdominal desmoid tumors in familial adenomatous polyposis: How much do clinical and surgical variables interfere with their development?
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Campos, Fábio Guilherme

; Martinez, Carlos Augusto Real

; Bustamante-Lopez, Leonardo Alfonso

; Mendonça, Roberta Laís da Silva

; Kanno, Danillo Toshio

Clinics

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2023, Volume 78 elocation 100144

Abstract: En | Text: En | PDF: En

Abstract Objective: Familial Adenomatous Polyposis is a complex hereditary disease that exposes the carrier to a great risk of Colorectal Cancer (CRC). After prophylactic surgery, intra-abdominal desmoid tumors are known to be one the most important cause of death. Therefore, recognition of increased-risk patients and modification of operative strategy may be crucial. Aim: The objective of this study was to estimate the desmoid tumor risk in relation to various surgical and clinical variables. Methods: Patients who had undergone polyposis since 1958 were included in the study. After exclusion criteria were met, those who had developed desmoid tumors were selected to undergo further evaluation. Results: The study revealed that the risk of developing desmoid tumors was associated with various factors such as sex ratio, colectomy, and reoperations. On the other hand, the type of surgery, family history, and surgical approach did not affect the risk of developing desmoid tumors. The data collected from 146 polyposis patients revealed that 16% had desmoid polyps. The sex ratio was 7:1, and the median age at colectomy was 28.6 years. Family history, multiple abdominal operations, and reoperations were some of the characteristics that were common in desmoid patients. Conclusion: Recognition of clinical (female sex) and surgical (timing of surgery and previous reoperations) data as unfavorable variables associated with greater risk may be useful during the decision-making process.

https://doi.org/10.1016/j.clinsp.2022.100144

Chikungunya virus infection in the southernmost state of Brazil was characterised by self-limited transmission (2017-2019) and a larger 2021 outbreak
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Gregianini, Tatiana Schäffer

; Salvato, Richard Steiner

; Barcellos, Regina Bones

; Godinho, Fernanda Marques

; Ruivo, Amanda Pellenz

; de Melo, Viviane Horn

; Schroder, Júlio Augusto

; Martiny, Fernanda Letícia

; Möllmann, Erica Bortoli

; Favreto, Cátia

; Baethgen, Ludmila Fiorenzano

; Ferreira, Vithoria Pompermaier

; de Lima, Lívia Eidt

; Piazza, Cláudia Fasolo

; Machado, Taís Raquel Marcon

; Becker, Irina Marieta

; Ramos, Raquel Rocha

; Frölich, Guilherme Carey

; Rossetti, Alana Fraga

; Almeida, Lucas da Cunha

; Rodrigues, Tahiana Machado Antunes

; Bragança, Isabella Tabelli

; Campos, Aline Alves Scarpellini

; Manzoni, Verônica Baú

; Machado, Lais Ceschini

; da Silva, Luisa Maria Inácio

; de Oliveira, André Luiz Sá

; Paiva, Marcelo Henrique Santos

; Nunes, Zenaida Marion Alves

; de Almeida, Paula Rodrigues

; Demoliner, Meriane

; Gularte, Juliana Schons

; da Silva, Mariana Soares

; Filippi, Micheli

; Pereira, Vyctoria Malayhka de Abreu Góes

; Spilki, Fernando Rosado

; da Veiga, Ana Beatriz Gorini

; Wallau, Gabriel Luz

Memórias do Instituto Oswaldo Cruz

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2023, Volume 118 elocation e220259

Abstract: En | Text: En | PDF: En

BACKGROUND Chikungunya is a mosquito-borne virus that has been causing large outbreaks in the Americas since 2014. In Brazil, Asian-Caribbean (AC) and East-Central-South-African (ECSA) genotypes have been detected and lead to large outbreaks in several Brazilian states. In Rio Grande do Sul (RS), the southernmost state of Brazil, the first cases were reported in 2016. OBJECTIVES AND METHODS We employed genome sequencing and epidemiological investigation to characterise the Chikungunya fever (CHIKF) burden in RS between 2017-2021. FINDINGS We detected an increasing CHIKF burden linked to travel associated introductions and communitary transmission of distinct lineages of the ECSA genotype during this period. MAIN CONCLUSIONS Until 2020, CHIKV introductions were most travel associated and transmission was limited. Then, in 2021, the largest outbreak occurred in the state associated with the introduction of a new ECSA lineage. CHIKV outbreaks are likely to occur in the near future due to abundant competent vectors and a susceptible population, exposing more than 11 million inhabitants to an increasing infection risk.

https://doi.org/10.1590/0074-02760220259

Mucin levels in glands of the colonic mucosa of rats with diversion colitis subjected to enemas containing sucralfate and n-acetylcysteine alone or in combination
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Brasil, Verena Palmeiras

; Siqueira, Rayama Moreira

; Campos, Fabio Guilherme

; Yoshitani, Mateus Magami

; Pereira, Geovanna Pacciulli

; Mendonça, Roberta Laís dos Santos

; Kanno, Danilo Toshio

; Pereira, José Aires

; Martinez, Carlos Augusto Real

Acta Cirúrgica Brasileira

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2023, Volume 38 elocation e384023

Abstract: En | Text: En | PDF: En

ABSTRACT Purpose: To evaluate the tissue content of neutral and acidic mucins, sulfomucins and sialomucins in colonic glands devoid of intestinal transit after enemas containing sucralfate and n-acetylcysteine alone or in combination. Methods: Sixty-four rats underwent intestinal transit bypass. A colonic segment was collected to compose the white group (without intervention). After derivation, the animals were divided into two groups according to whether enemas were performed daily for two or four weeks. Each group was subdivided into four subgroups according to the substance used: control group: saline 0.9%; sucralfate group (SCF): SCF 2 g/kg/day; n-acetylcysteine group (NAC): NAC 100 mg/kg/day; and SCF+NAC group: SCF 2 g/kg/day + NAC 100 mg/kg/day.Neutral and acidic mucins were stained by periodic acid-Schiff and alcian-blue techniques, respectively. The distinction between sulfomucins and sialomucin was made by the high alcian-blue iron diamine technique. The content of mucins in the colonic glands was measured by computerized morphometry. The inflammatory score was assessed using a validated scale. The results between the groups were compared by the Mann-Whitney’s test, while the variation according to time by the Kruskal-Wallis’ test (Dunn’s post-test). A significance level of 5% was adopted. Results: There was reduction in the inflammatory score regardless of the application of isolated or associated substances. Intervention with SCF+NAC increased the content of all mucin subtypes regardless of intervention time. Conclusions: The application of SCF+NAC reduced the inflammatory process of the colonic mucosa and increased the content of different types of mucins in the colonic glands of segments excluded from fecal transit.

https://doi.org/10.1590/acb384023

O Instituto Jean Jacques Rousseau e o Brasil: vestígios de uma história conectada
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Azevedo, Laís Paula de Medeiros Campos

; Neta, Olívia Morais de Medeiros

Educar em Revista

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2023, Volume 39 elocation e86102

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT The Jean Jacques Rousseau Institute (JJRI) was created in 1912, in Geneva, Switzerland, with the aim of promoting new horizons and practices in education. The defense for the diffusion of a pedagogical science, based on the studies of experimental and childhood Psychology, guided the actions of the Institute which worked, especially, through teachers’ training. The Institute’s courses received students and visitors from several countries, including Brazil. In this study, we seek to investigate the traces of the networks built among the Institute and intellectuals of Brazilian Education, from its creation until the mid-1930s. We adopt as main sources the newspapers and magazines available in the “Hemoteca Digital Brasileira”. Our theoretical and methodological contribution is based on the works of Ginzburg (2007), Mignot and Gondra (2007), Houssaye (2007), Fuchs (2007), Roldán Vera and Fuchs (2021), Vidal and Rabelo (2020) and Rabelo and Vidal (2021). We emphasize that the Institute’s performance sought to create a network of educators from several countries in order to spread an international movement for the transformation of education, associated with the New School movement. Our study showed evidence on the connection among the Institute, intellectuals, and Brazilian institutions, pointing out that the educational perspectives defended by the Institute were spread in Brazil by teachers who not only visited, but also worked in the country during the period investigated in the dissemination of ideals expressed in training courses by the JJRI.

RESUMO O Instituto Jean Jacques Rousseau (IJJR) foi criado no ano de 1912, em Genebra - Suíça, com o objetivo de promover novos horizontes e práticas para a educação. A defesa pela difusão de uma ciência pedagógica, pautada nos estudos da Psicologia experimental e da infância, nortearam as ações do Instituto que atuava, principalmente, por meio da formação de professores. Seus cursos receberam estudantes e visitantes de diversos países, inclusive brasileiros. Nesse estudo, buscamos investigar os vestígios das redes construídas entre o Instituto e os intelectuais da educação brasileira, desde a sua criação até meados da década de 1930. Adotamos como fontes principais os jornais e as revistas disponibilizados na Hemeroteca Digital Brasileira. Nosso aporte teórico e metodológico constitui-se a partir das contribuições de Ginzburg (2007), Mignot e Gondra (2007), Houssaye (2007), Fuchs (2007), Roldán Vera e Fuchs (2021), Vidal e Rabelo (2020) e Rabelo e Vidal (2021). Destacamos que a atuação do Instituto buscava a construção de uma rede de educadores de vários países no intuito da disseminação de um movimento internacional de transformação da educação, associado ao movimento da Escola Nova. Nosso estudo demonstrou indícios da conexão entre o Instituto, intelectuais e instituições brasileiras, apontando para que as perspectivas educacionais defendidas no Instituto circularam no Brasil por meio de professores que não apenas visitaram, mas atuaram no país no período investigado na divulgação de ideais expressos em cursos de formação pelo IJJR.

https://doi.org/10.1590/1984-0411.86102

Determining reference ranges for immunological cells of healthy indigenous individuals from a region in Brazil
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Morais, Isa Rita Brito de

; Barbosa, Dyjaene de Oliveira

; Almeida, Gabriel Barroso de

; Costa, Regina Rossoni da

; Silva, Bruna Oliveira da

; Oliveira, Laís Albuquerque de

; Arantes, Julia Pimentel

; Leite, Layla Oliveira Campos

; Rossato, Luana

; Ribeiro, Marcos Borges

; Marchioro, Silvana Beutinger

; Freire, Songelí Menezes

; Nascimento, Roberto José Meyer

; Simionatto, Simone

; Torres, Alex José Leite

einstein (São Paulo)

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2023, Volume 21 elocation eAO0291

Abstract: En | Text: En | PDF: En

ABSTRACT Objective The establishment of reference values for a subset of leukocytes is common in clinical practice, and ethnic variations are strongly associated with disease development. In Brazil, indigenous people are vulnerable to infections, and few studies have described the health and disease conditions of this population. This study aimed to provide reference values for immunological cell subsets in indigenous Brazilians living in the state of Mato Grosso do Sul. Methods Flow cytometry and 4-color combinations of monoclonal antibodies were used to characterize cells. A total of 115 healthy adults, mostly females (72%), were included in the study. The results are presented as mean and median (2.5%-97.5% percentiles) for T and B lymphocytes, CD4+ T cells, CD8+ T cells, Natural Killer cells, monocytes, and dendritic cells, providing an average immunological profile for the population in question. Results The relative medians of CD3+, CD4+, and CD8+ T cells were significantly higher in women than in men in a healthy indigenous population. Conclusion To our knowledge, cell reference data from indigenous Brazilians are unknown in the literature. The immune cell results presented in this pioneering study will contribute to the clinical and laboratory evaluation of the Brazilian indigenous population, especially given the important differences when compared with other Brazilian ethnic groups.

https://doi.org/10.31744/einstein_journal/2023ao0291

Lei Geral de Proteção de Dados (LGPD) e repositórios institucionais: reflexões e adequações LGPD (LGPD institucionais
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Nascimento, Bruna Laís Campos do

; Silva, Edilene Maria da

Em Questão

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2023, Volume 29 elocation e-127314

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo: Este artigo discute a Lei Geral de Proteção de Dados no contexto dos repositórios institucionais, enfocando as adequações necessárias a serem realizadas. Apresenta um breve panorama da legislação brasileira que trata sobre a privacidade e a proteção de dados. O presente trabalho caracteriza-se quanto aos fins como uma pesquisa de natureza exploratória, e quanto aos meios como bibliográfica e documental, com abordagem qualitativa. A partir das análises, foi identificado que a Lei Geral de Proteção de Dados apresenta consistentes diretrizes e sanções para que as instituições possam garantir adequados tratamento e segurança dos dados pessoais. Quanto aos repositórios, foi percebido que adaptações são necessárias e o estudo propõe algumas recomendações, principalmente no que concerne aos processos e fluxos de trabalho, à revisão de documentos institucionais, capacitação e às ações de transparência. Conclui que as discussões sobre a privacidade e a proteção dos dados pessoais precisam ser fomentadas na sociedade, incluindo os repositórios nesse cenário de aplicação. Resumo institucionais realizadas caracterizase caracteriza se exploratória documental qualitativa análises recomendações transparência sociedade aplicação

Abstract: This article discusses the General Data Protection Law in the context of institutional repositories. It focuses on the necessary adjustments to be done. This study presents a brief overview of Brazilian legislation about privacy and data protection. This research is characterized as exploratory by its ends, bibliographic and documentary by its means, and it has a qualitative approach. The results demonstrates that the General Data Protection Law presents consistent guidelines and sanctions so that institutions can guarantee an adequate treatment and security of personal data. The repositories need to be adapted regarding data protection. The study proposes recommendations about processes and workflows, such as review of institutional documents, training, and transparency actions. This study concludes that discussions about privacy and protection of personal data need to be encouraged in society, including the repositories in this application scenario. Abstract done ends means approach workflows documents training actions society scenario

https://doi.org/10.1590/1808-5245.29.127314

Por que devemos nos preocupar com os bebês a termo internados em uma unidade de terapia intensiva neonatal
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Lucas, Taís Queiroz Campos

; Mendelski, Alexandra Quines

; Almeida, Carla Skilhan de

; Gerzson, Laís Rodrigues

Fisioterapia e Pesquisa

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aug 2022, Volume 29 N. 2 Pages 181 - 188

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO O objetivo deste estudo foi analisar a razão pela qual devemos nos preocuparmos com os bebês a termo internados em uma unidade de terapia intensiva neonatal. Trata-se de estudo documental, descritivo e retrospectivo de 262 recém-nascidos (RNs) a termo. As variáveis utilizadas foram: características dos RN; diagnóstico principal, tempo de permanência e acompanhamento pela equipe multiprofissional; e encaminhamento pós-alta. Houve prevalência do sexo masculino (52%), de Apgar 9 no 5º minuto e da raça/cor branca do RN e da mãe (61,1% e 48,9%, respectivamente). O diagnóstico principal foi a disfunção respiratória (28,8%), e o tempo de permanência foi de oito dias. Houve diferença significativa entre os tempos de permanência (p=0,013), em que as doenças cardiorrespiratórias e outras doenças levaram a um menor tempo de internação em relação à má formação ou às doenças maternas. O serviço social foi o mais procurado para o acompanhamento (81,2%) e a fisioterapia, o menos buscado (18%). RNs com maior peso ficaram menos tempo internados, e os acompanhados por fisioterapia apresentaram tempo de permanência mais elevados (p<0,001). O principal desfecho foi a alta hospitalar (68,7%) e encaminhamentos para a Unidade Básica de Saúde (57%). Os achados deste estudo apontam a presença de bebês menos graves, baixo número de estudos específicos para a população a termo e outros diagnósticos que nos remetem a cuidados não intensivos.

ABSTRACT This study aims to analyze why we should care about full-term newborns admitted to a neonatal intensive care unit. This is a documented, descriptive, and retrospective study of 262 full-term newborns. Variables used: newborns’ characteristics; main diagnosis, length of stay, follow-up by a multidisciplinary team; post-discharge referral. Most newborns were boys (52%), had a 5-minute Apgar score of nine, and most newborns and their mothers were white (61.1% and 48.9% respectively). Respiratory dysfunction was the main diagnosis (28.8%). Length of stay was eight days. There was a significant difference regarding length of stay (p=0.013), in which those with cardiorespiratory and other diseases stayed less time compared to those with malformation or maternal diseases. The social service was the most sought (81.2%) service, whereas physical therapy the least sought (18%). Newborns with higher weight were hospitalized for less time. Those that underwent physical therapy had longer stay (p<0.001). Main outcome was hospital discharge (68.7%) and referrals to the Basic Health Unit (57%). This study outcomes indicated newborns with less severe conditions, low number of specific studies for the full-term population, other diagnoses that refer to non-intensive care.

RESUMEN El objetivo de este estudio fue analizar el motivo de preocupación por los recién nacidos a término ingresados en una unidad de cuidados intensivos neonatal. Se trata de un estudio documental, descriptivo y retrospectivo, realizado con 262 recién nacidos (RN) a término. Las variables utilizadas fueron: características de los RN; diagnóstico principal, tiempo de estancia y seguimiento por el equipo multidisciplinar; y derivación posterior al alta. Hubo predominio del sexo masculino (52%), Apgar 9 al 5º minuto y raza/color blanca del RN y de la madre (61,1% y 48,9%, respectivamente). El principal diagnóstico fue disfunción respiratoria (28,8%), y la estancia hospitalaria fue de ocho días. Hubo una diferencia significativa entre el tiempo de estancia (p=0,013), en que las enfermedades cardiorrespiratorias y otras enfermedades resultaron en una menor estancia hospitalaria con relación a malformaciones o enfermedades maternas. El trabajo social fue el más buscado para el seguimiento (81,2%), y la fisioterapia, el menos buscado (18%). Los RN con mayor peso tuvieron una menor estancia hospitalaria, y aquellos que recibían seguimiento de fisioterapia tuvieron mayor tiempo de estancia (p<0,001). El principal desenlace fue el alta hospitalaria (68,7%) y las derivaciones a la Unidad Básica de Salud (57%). Los hallazgos de este estudio apuntan a la presencia de recién nacidos menos graves, un bajo número de estudios específicos para la población a término y otros diagnósticos que nos remiten a cuidados no intensivos.

https://doi.org/10.1590/1809-2950/21023029022022pt

Postharvest quality of two sweet potato cultivars submitted to different harvest ages
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Silva, Thayane de A

; Neves, Leandro TBC

; Campos, Daniela C dos S

; Carvalho, Laís de B

; Sousa, Lailson O de

; Sousa, Jaine F de

; Silva, Anderson N

Horticultura Brasileira

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jun 2022, Volume 40 N. 2 Pages 214 - 220

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A qualidade das raízes de batata-doce é influenciada por fatores que antecedem a colheita. Assim, objetivou-se na execução deste trabalho a correta determinação do ponto de colheita e o estudo da qualidade pós-colheita de duas cultivares de batata-doce colhidas em diferentes idades de colheita. O experimento foi realizado nas dependências da Universidade Federal de Roraima. As plantas foram cultivadas de abril a agosto, durante o período chuvoso de 2020. A colheita foi realizada aos 80, 95, 110, 125 e 140 dias após o plantio (DAP). As raízes foram higienizadas e em seguida foram avaliadas as características físico-químicas da polpa e casca. Os sólidos solúveis, acidez titulável e relação SS/AT foram observados em maior quantidade na cultivar BD-02. A luminosidade e croma da casca das raízes tuberosas de batata-doce foram mais intensas na cultivar BD-01 e o inverso foi observado na caracterização da polpa da cultivar BD-01. Aos 110 dias após plantio (DAP) observou-se os maiores teores de açúcares redutores e croma da casca; menores valores de croma da polpa e °H da casca. Dessa forma, segundo os resultados acima apresentados, especialmente em função do metabolismo dos carboidratos e a observação da produtividade e o acúmulo de matéria seca, a cultivar BD-02, colhida aos 110 DAP, apresentou as melhores características pós-colheita nas condições experimentais aqui apresentadas.

ABSTRACT The quality of sweet potato roots is influenced by factors which precede harvest. Thus, the aim of this study was to correctly determine the harvest point and study the postharvest quality of two sweet potato cultivars harvested at different ages. The experiment was carried out at Universidade Federal de Roraima. The plants were grown from April to August, during rainy season, 2020. The harvest was carried out at 80, 95, 110, 125 and 140 days after planting (DAP). The tuberous roots were cleaned and the physicochemical traits of the pulp and peel were evaluated. Soluble solids, titratable acidity and SS/AT ratio were observed in greater quantity in the cultivar BD-02. The luminosity and chroma of the peel of the sweet potato roots were more intense in the cultivar BD-01 and the opposite was observed when characterizing the pulp of this cultivar. At 110 DAP, we verified the highest levels of reducing sugars and chroma of the peel, as well as lower values for the chroma of the pulp and °H of the peel. Thus, according to the results presented above, especially in relation to carbohydrate metabolism, productivity and dry matter accumulation, the cultivar BD-02, harvested at 110 DAP, showed the best postharvest traits under the experimental conditions presented in this study.

https://doi.org/10.1590/s0102-0536-20220211

10.

Evaluation of different silvicultural management techniques and water seasonality on yield of eucalyptus stands
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Silva, Laís Graziele

; Santana, Reynaldo Campos

; Oliveira, Marcio Leles Romarco de

; Gomes, Francisco Sérgio

Scientia Agricola

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2022, Volume 79 N. 2 elocation e20200064

Abstract: En | Text: En | PDF: En

ABSTRACT: Appropriate management of factors that influence forest development is essential to increase yield of forest plantations. The objective of this study was to evaluate the influence of water seasonality, nutritional management, and uniformity on yield of eucalyptus plantations and estimate the potential, attainable, and observed yield of adult eucalyptus stands. We evaluated Eucalyptus clonal stands in six regions of Minas Gerais State, Brazil, in a system of first and second rotation across four age classes using the twin-plots method (TP). In the study, 142 TPs were allocated alongside 142 plots in a continuous forest inventory (CFI) network of a private enterprise. The CFI received operational fertilization and additional fertilization was carried out in the TP. The trees were measured at the beginning of the experiment and at every six months to quantify the production in the wet (PW) and dry (PD) periods of the year. Uniformity of plantations was evaluated using Pvar 50 % and the optimal uniformity index. Potential, attainable, and observed yields were estimated using the average annual increase at seven years of age. The PW showed higher plant yield. There was an effect due to water availability and nutrient level on the yield of the stands. The driest semester of the year produces approximately 30 % of the current annual increase. The stands under the second rotation tend to have less uniformity than the in the first rotation. Potential yield varied depending on rainfall intensity where wetter regions had the highest yield.

https://doi.org/10.1590/1678-992x-2020-0064 200 downloads

11.

Genetic diversity in Amazonian Jundiá (Leiarius marmoratus) stocks using heterologous primers
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Mantovani, Laís Santana Celestino

; Souza, Felipe Pinheiro de

; Campos, Eric Costa

; Lewandowski, Vanessa

; Barrero, Nelson Maurício Lopera

; Ribeiro, Ricardo Pereira

Acta Scientiarum. Animal Sciences

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2022, Volume 44 elocation e52657

Abstract: En | Text: En | PDF: En

ABSTRACT. With the rise of world fish farming, the national scenario is favorable for using native fish for intensive farming. Among the catfish, the Amazonian Jundiá (Leiarius marmoratus) is a robust candidate, easy to grow and with good organoleptic characteristics in its flesh. For productive success in captivity, it is necessary to consider some questions about the species, such as genetic variability, which must have an acceptable level in a breeding stock, in order to maintain a good diversity; this reduces losses due to inbreeding and low diversity. Therefore, the objective of this study was to characterize the genetic variability of commercial stocks of L. marmoratus from the State of Mato Grosso through microsatellite molecular markers. We analyzed 143 individuals from three stocks. The mean heterozygosity and the inbreeding coefficients observed were 0.060; 0.084; 0.141; and 0.539; 0.562; 0.514, respectively, for the stocks of Campo Verde, Juína, and Nova Mutum. The Deviation in the Hardy-Weinberg equilibrium was observed in most of the loci in the three populations. Considering the genetic differentiation, it is concluded that the three populations are very close genetically, which requires introduction of new genetic material in the stocks to enrich them genetically for a later reproductive program.

https://doi.org/10.4025/actascianimsci.v44i1.52657

12.

Simultaneous extraction of carotenoids and phenolic compounds from pulps of orange and yellow peach palm fruits (Bactris gasipaes) by ultrasound-assisted extraction
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MONTEIRO, Sara Fonseca

; COSTA, Evellyn Laís Neves

; FERREIRA, Ramon Sousa Barros

; CHISTÉ, Renan Campos

Food Science and Technology

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2022, Volume 42 elocation e34021

Abstract: En | Text: En | PDF: En

Abstract Peach palm fruits (Bactris gasipaes) are native to the Amazonia and exhibit high levels of bioactive compounds, especially carotenoids and phenolic compounds. In our study, the ultrasound-assisted extraction (UAE) technique with different solvents [ethanol, ethyl acetate and ethanol/water (1:1, v/v)] was investigated to determine the best conditions for the simultaneous extraction of carotenoids and phenolic compounds from pulps of orange and yellow peach palm fruits to produce extracts with high bioactive compound contents. During the UAE experiments, the number of extraction and solid-liquid ratio were the variables that most affected bioactive compounds extraction (p < 0.05) and ethanol allowed the extraction of the highest carotenoid contents, while ethanol/water was the most efficient solvent to extract phenolic compounds. Through response surface methodology, ethanol/water resulted in optimal conditions at 10 extractions of 2 min each at a solid-liquid ratio of 1:12 (w/v) to extract phenolic compounds. Notwithstanding, the simultaneous extraction of carotenoids and phenolic compounds with ethanol and ethyl acetate was observed to be achieved at the same UAE conditions for both fruit varieties. Regardless the solvent, the UAE proved to be an efficient technique to carry out simultaneous extraction of high contents of carotenoids and phenolic compounds from pulps of peach palm fruits.

https://doi.org/10.1590/fst.34021

13.

Presence of Civatte Bodies in an oral lichen planus incisional biopsy sample
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SANCHES, Ana Carla Barletta

; PIRES, Alessandra Laís Pinho Valente

; RAMOS, Tarsila de Carvalho Freitas

; FROTA, Beatriz de Araújo

; DELLA CELLA, Hortencia Resende dos Santos

; OLIVEIRA, Márcio Campos

; FREITAS, Valéria Souza

; MARTINS, Gabriela Botelho

RGO - Revista Gaúcha de Odontologia

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2022, Volume 70 elocation e20220014

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: Corpos de Civatte são corpos coloidais de queratinócitos apoptóticos localizados na junção dermoepidérmica e que são frequentes em várias dermatoses, inclusive as lesões de líquen plano. O objetivo do presente trabalho foi determinar a presença de corpos de Civatte em uma amostra de biópsias incisionais obtidas a partir de pacientes com diagnóstico de líquen plano oral (LPO). Métodos: Tratou-se de um estudo transversal retrospectivo, realizado com 34 casos cujas lâminas foram coradas pela hematoxilina-eosina, pertencentes ao arquivo de um Laboratório de Patologia Oral de Instituição de Ensino Superior. A amostra obtida foi classificada em lesões brancas e vermelhas a partir dos dados clínicos disponíveis. As análises histológicas foram realizadas em microscópio de luz, nas objetivas de 10x e 40x, com identificação dos corpos de Civatte como presentes ou ausentes. Resultados: Os corpos colóides estavam presentes em 73,5% da amostra total estudada e em 74,2% das lesões brancas. Conclusão: Os corpos de Civatte foram frequentes em todas as amostras analisadas e não devem ser negligenciados como critério de diagnóstico histopatológico em lesões de LPO.

ABSTRACT Objective: Civatte bodies are colloid bodies of apoptotic keratinocytes located at the dermoepidermal junction and are common in several dermatoses, including lichen planus lesions. The present study aimed to determine the presence of Civatte bodies in a sample of incisional biopsies obtained from patients diagnosed with oral lichen planus. Methods: A retrospective cross-sectional study, carried out with 34 slides stained with hematoxylin-eosin from the archive of an Oral Pathology Laboratory of a Higher Education Institution. The sample obtained was classified into white and red lesions from the available clinical data. Histological analyses were performed under a light microscope, with 10x and 40x objectives, identifying the presence or absence of Civatte bodies. Results: Colloid bodies were present in 73.5% the total sample studied and in 74.2% white lesions. Conclusion: The Civatte bodies were frequent in all samples analysed and should thus be considered a criterion for histological diagnosis in lesions of Oral Lichen Planus.

https://doi.org/10.1590/1981-86372022001420200225

14.

Physiotherapist on the move: where babies at risk are referred for follow-up after hospital discharge
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Mendelski, Alexandra Quines

; Lucas, Taís Queiroz Campos

; Almeida, Carla Skilhan de

; Gerzson, Laís Rodrigues

Fisioterapia em Movimento

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2022, Volume 35 elocation e35134

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Introdução Dois fatos podem influenciar o desenvolvimento do recém-nascido. Um é ser um recém-nascido de risco e o outro é ser internado em Unidade de Terapia Intensiva. Objetivo Examinar para onde os bebês de risco estão sendo encaminhados para acompanhamento longitudinal após a alta hospitalar e realizar uma análise do perfil epidemiológico e assistencial desses bebês. Métodos Estudo documental, descritivo e retrospectivo, composto pelos prontuários de 479 recém-nascidos (RNs) internados no Hospital Materno Infantil Presidente Vargas no período de janeiro de 2019 a maio de 2020. As variáveis estudadas foram: sexo, raça/cor do bebê, tipo de parto, consultas de pré-natal, classificação de acordo com a idade gestacional idade, peso, Apgar do 1º, 5º, 10º minuto, diagnóstico de internação, diagnóstico principal, desfechos, tempo de internação, acompanhamento multidisciplinar durante a internação, encaminhamentos pós-alta (especializados e não especializados). Os dados socioambientais foram: idade materna e raça/cor, escolaridade materna e paterna. Resultados Maior prevalência de bebês a termo do sexo masculino, nascidos de parto cesáreo, declarados como brancos, com escores de Apgar elevados, com diagnósticos variados, prevalecendo a pre-maturidade. A média materna foi de 26,2 anos (DP ± 7,3), a raça/cor mais relatada foi branca, a média de estudos das mães foi de 8,1 anos (DP ± 2,4). Apenas 14% (n = 67) realizaram fisioterapia motora no hospital e 2,1% (n = 10) foram encaminhados para avaliação e intervenção precoce para fisioterapia pós-alta. O serviço especializado de maior referência foi o ambulatório de egressos de neuropediatria do hospital (17,3%, n = 83) e, para os não especializados, foi a Unidade Básica de Saúde/UBS (39,7%, n = 190). Conclusão A maioria dos RNs são encaminhados para equipe médica específica ou UBS pós-alta. O fisioterapeuta foi o profissional pouco lembrado pelo acompanhamento desse público no hospital e após a alta.

Abstract Introduction Two facts may influence a newborn’s development. One is to be a newborn at risk and the other is to be hospitalized in Intensive Care Unit. Objective To examine where at-risk infants are being referred for longitudinal follow-up after hospital discharge, and to carry out an analysis of the epidemiological and care profile of these babies. Methods Documentary, descriptive and retrospective study, comprising the medical records of 479 newborns (NBs) hospitalized Hospital Materno Infantil Presidente Vargas from January 2019 to May 2020. The variables studied were: gender, baby's race/color, type of delivery, prenatal consultations, classification according to gestational age, weight, Apgar of the 1st, 5th, 10th minute, hospitalization diagnosis, main diagnosis, outcomes, length of stay, multidisciplinary follow-up during hospitalization, post-discharge referrals (specialized and non-specialized). Social and environmental data were: maternal age and race/color, maternal and paternal education. Results Higher prevalence of full-term male babies born by cesarean delivery, declared as white, with high Apgar scores, with varied diagnoses, with prematurity prevailing. Maternal mean was 26.2 years, (SD ± 7.3), the most reported race/color was also white, the mean of mothers' studies was 8.1 years (SD ± 2.4). Only 14% (n = 67) performed motor physical therapy at the hospital and 2.1% (n = 10) were referred for evaluation and early intervention for post-discharge physical therapy. The specialized service with the highest referral was the hospital's neuropediatrics graduates' outpatient clinic (17.3%, n = 83) and, for the non-specialized, it was the Basic Health Unit/BHU (39.7%, n = 190). Conclusion Most NBs are referred to the specific medical team or post-discharge BHU. The physiotherapist was the professional little remembered for monitoring this public in the hospital and after discharge.

https://doi.org/10.1590/fm.2022.35134

15.

MULTIPLE NEUROENDOCRINE NEOPLASIA IN A PATIENT WITH TYPE I NEUROFIBROMATOSIS (NF1): REPORT OF A NEW MUTATION (NF1, EXONS 2-30 DELETION) AND LITERATURE REVIEW
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KANNO, Danilo Toshio

; MATTOS, Roberta Lais Mendonça de

; CAMPOS, Fábio Guilherme

; SIQUEIRA, Rayama Moreira

; CARVALHO, Rita Barbosa de

; REAL MARTINEZ, Carlos Augusto

ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo)

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2022, Volume 35 elocation e1702

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT BACKGROUND: Plexiform neurofibromas represent a common neoplasia of type 1 neurofibromatosis in which neurofibromas arise from multiple nerves involving connective tissue and skin and rarely affect the colon and rectum. Co-occurrence of plexiform neurofibromas, neuroendocrine tumors with primary involvement of the rectum, and medullary thyroid carcinoma in patients with neurofibromatosis type 1 is a previously undescribed condition. The aim of this manuscript was to present a case of primary plexiform neurofibroma and neuroendocrine tumors of the upper rectum in a patient with neurofibromatosis type 1 whose genetic sequencing found a novel mutation in the neurofibromatosis type 1 gene and to review the literature. CASE REPORT: A 49-year-old woman with a familial history of neurofibromatosis type 1 complained of abdominal cramps for 6 months. She had previously been submitted for a total thyroidectomy due to medullary thyroid carcinoma. She was submitted to a colonoscopy, which identified a submucosa lesion located in the upper rectum. The patient was referred for a laparoscopic rectosigmoidectomy, and the histopathological study of the surgical specimen identified two different tumors. An immunohistochemical panel was done for histopathological confirmation of the etiology of both lesions. The results of the panel showed intense immunoexpression of S100 protein in the largest and superficial lesion, as well as positivity for chromogranin and synaptophysin in the minor and deep lesion confirming the diagnosis of rectal plexiform neurofibromas concomitant with neuroendocrine tumors. The proliferative activity rate using Ki-67 antibodies showed that both tumors had a low rate of mitotic activity (<1%). Genetic sequence panel identified an undescribed mutation in the neurofibromatosis type 1 gene (deletion, exons 2–30). The patient’s postoperative evolution was uneventful, and she remains well, without recurrence, 3 years after surgery. CONCLUSION: The co-occurrence of medullary thyroid carcinoma, plexiform neurofibromas, and neuroendocrine tumors of the rectum in patients with neurofibromatosis type 1 is an exceptional and undescribed possibility, whose diagnosis can be confirmed by the immunohistochemical staining and genetic panel.

RESUMO RACIONAL: Os neurofibromas plexiformes representam uma neoplasia frequente na neurofibromatose tipo 1 onde os neurofibromas surgem de múltiplos nervos envolvendo, também, o tecido conjuntivo e a pele raramente acometendo o cólon e o reto. A co-ocorrência de neurofibromas plexiformes, tumores neuroendócrinos, com envolvimento primário do reto, e carcinoma medular de tireoide em pacientes com neurofibromatose tipo 1 é uma condição ainda não descrita. O objetivo deste manuscrito é descrever um caso de neurofibromas plexiformes e tumores neuroendócrinos primários do reto superior, em paciente com neurofibromatose tipo 1, cujo sequenciamento genético identificou uma nova mutação no gene neurofibromatose tipo 1 e revisar a literatura sobre o tema. DESCRIÇÃO DO CASO: Mulher de 49 anos, com história familiar de neurofibromatose tipo 1 queixava-se de cólicas abdominais há seis meses. Tinha sido previamente submetida a tireoidectomia total por carcinoma medular de tireoide. Foi submetida a colonoscopia, que identificou lesão submucosa localizada no reto superior. A doente foi encaminhada para retossigmoidectomia laparoscópica, cujo estudo histopatológico do espécime cirúrgico identificou dois tumores distintos. Realizou-se painel imuno-histoquímico para confirmação histopatológica da etiologia de ambas as lesões. O resultado do painel mostrou intensa imunoexpressão da proteína S-100 da lesão superficial e de maior tamanho,e positividade para cromogranina e somatofisina da lesão mais profunda e de menores dimensões confirmando o diagnóstico de neurofibromas plexiformes retal e tumores neuroendócrinos. O índice de atividade proliferativa utilizando o anticorpo ant-Ki-67 mostrou que ambos os tumores apresentavam baixa índice mitótico (<1%). O painel genético identificou mutação no gene neurofibromatose tipo 1 (deleção, exons 2–30) ainda não descrita. A doente apresentou evolução pós-operatória sem intercorrências, permanecendo bem, sem recorrência, três anos após a cirurgia. CONCLUSÕES: A co-ocorrência de carcinoma medular de tireoide, neurofibromas plexiformes e tumores neuroendócrinos do reto em pacientes com neurofibromatose tipo 1 é uma possibilidade excepcional e ainda não descrita, cujo diagnóstico pode ser confirmado pela coloração imuno-histoquímica e painel genético.

https://doi.org/10.1590/0102-672020220002e1702

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