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1.
Aging-related predictive factors for oxygenation improvement and mortality in COVID-19 and acute respiratory distress syndrome (ARDS) patients exposed to prone position: A multicenter cohort study
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Cunha, Marieta C.A.
; Schardong, Jociane
; Righi, Natiele C.
; Lunardi, Adriana C.
; Sant’Anna, Guadalupe N.
; Isensee, Larissa P.
; Xavier, Rafaela F.
; Pompeu, Jose E.
; Weigert, Renata M.
; Matte, Darlan L.
; Cardoso, Rozana A.
; Abras, Ana C.V.
; Silva, Antonio M.V.
; Dorneles, Camila C.
; Werle, Roberta W.
; Starke, Ana C.
; Ferreira, Juliana C.
; Plentz, Rodrigo D.M.
; Carvalho, Celso R.F.
.
Abstract Background: Elderly patients are more susceptible to Coronavirus Disease-2019 (COVID-19) and are more likely to develop it in severe forms, (e.g., Acute Respiratory Distress Syndrome [ARDS]). Prone positioning is a treatment strategy for severe ARDS; however, its response in the elderly population remains poorly understood. The main objective was to evaluate the predictive response and mortality of elderly patients exposed to prone positioning due to ARDS-COVID-19. Methods: This retrospective multicenter cohort study involved 223 patients aged ≥ 65 years, who received prone position sessions for severe ARDS due to COVID-19, using invasive mechanical ventilation. The PaO2/FiO2 ratio was used to assess the oxygenation response. The 20-point improvement in PaO2/FiO2 after the first prone session was considered for good response. Data were collected from electronic medical records, including demographic data, laboratory/image exams, complications, comorbidities, SAPS III and SOFA scores, use of anticoagulants and vasopressors, ventilator settings, and respiratory system mechanics. Mortality was defined as deaths that occurred until hospital discharge. Results: Most patients were male, with arterial hypertension and diabetes mellitus as the most prevalent comorbidities. The non-responders group had higher SAPS III and SOFA scores, and a higher incidence of complications. There was no difference in mortality rate. A lower SAPS III score was a predictor of oxygenation response, and the male sex was a risk predictor of mortality. Conclusion: The present study suggests the oxygenation response to prone positioning in elderly patients with severe COVID-19-ARDS correlates with the SAPS III score. Furthermore, the male sex is a risk predictor of mortality.
2.
Sustainable nitrogen efficiency in wheat by the dose and mode of supply
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Trautmann, Ana P. B.
; Silva, José A. G. da
; Carvalho, Ivan R.
; Colet, Christiane de F.
; Lucchese, Osório A.
; Basso, Natiane C. F.
; Alessi, Odenis
; Sgarbossa, Jaqueline
; Dias, Jéssica E. L.
; Peter, Cibele L.
.
Revista Brasileira de Engenharia Agrícola e Ambiental
- Journal Metrics
RESUMO O manejo do nitrogênio analisando indicadores agronômicos e ambientais garante produtividade satisfatória com maior qualidade ambiental. O objetivo neste estudo é propor um manejo mais sustentável de uso do nitrogênio em trigo, considerando o fornecimento em dose cheia e fracionada com estimativa da eficiência técnica, econômica e de expectativa de produtividade. Dois experimentos foram conduzidos, um para quantificar a produtividade de biomassa e outro, para avaliação do rendimento de grãos. O delineamento experimental foi o de blocos casualizados com quatro repetições em fatorial 5 × 3, para doses de N-fertilizante (0, 30, 60, 90 e 120 kg ha-1) e forma de fornecimento do nutriente [condição cheia (100%) no estádio fenológico V3 (terceira folha expandida); fracionada (70/30%) no estádio fenológico V3/V6 (terceira e sexta folha expandida) e; fracionada (70/30%) no estádio fenológico V3/R1 (início da antese)], respectivamente. O manejo mais sustentável do nitrogênio em trigo é obtido com a dose de expectativa de produtividade de 3 t ha-1 pela adubação manual, com o nutriente fornecido em dose cheia no estádio fenológico V3.
ABSTRACT The management of nitrogen by the assessment of agronomic and environmental indicators ensures satisfactory productivity with higher environmental quality. The objective of this study was to propose more sustainable management of nitrogen use in wheat, considering the supply of full and fractioned doses with an estimate of technical, economic efficiency, and expected productivity. Two experiments were conducted, one to quantify biomass productivity and the other to evaluate grain yield. The experimental design consisted of a randomized block with four repetitions in a 5 × 3 factorial, for N-fertilizer doses (0, 30, 60, 90, and 120 kg ha-1) and nutrient supply form [full condition (100%) in the phenological stage V3 (the third leaf expanded); fractionated (70/30%) in the phenological stage V3/V6 (third and sixth leaf expanded) and; fractionated (70/30%) in the phenological stage V3/R1 (beginning of anthesis)], respectively. The most sustainable management of nitrogen in wheat was obtained with the expected yield rate of 3 t ha-1 by manual fertilization, with nitrogen supplied in full dose at the V3 phenological stage.
3.
Correlations between Web Searches and COVID-19 Epidemiological Indicators in Brazil
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Locatelli, Marcelo Sartori
; Cunha, Evandro L. T. P
; Guiginski, Janaína
; Franco, Ramon A. S
; Bernardes, Tereza
; Alzamora, Pedro Loures
; Silva, Daniel Victor F. da
; Ganem, Marcelo Augusto S
; Santos, Thiago H. M
; Carvalho, Anne I. R
; Souza, Leandro M. V
; Paixão, Gabriela P. F
; Chaves, Elisa França
; Santos, Guilherme Bezerra dos
; Santos, Rafael Vinícius dos
; Freitas, Amanda Cupertino de
; Flores, Matheus G
; Biezuner, Rachel F
; Cardoso, Rodolfo Lins
; Fonseca, Rodrigo Machado
; Silva, Ana Paula Couto da
; Meira Jr, Wagner
.
Abstract: COVID-19 rapidly spread across the world in an unprecedented outbreak with a massive number of infected and fatalities. The pandemic was heavily discussed and searched on the internet, which generated big amounts of data related to it. This led to the possibility of attempting to forecast coronavirus indicators using the internet data. For this study, Google Trends statistics for 124 selected search terms related to pandemic were used in an attempt to find which keywords had the best Spearman correlations with a lag, as well as a forecasting model. It was found that keywords related to coronavirus testing among some others, such as “I have contracted covid”, had high correlations (≥0.7) with few weeks of lag (≤4 weeks). Besides that, the ARIMAX model using those keywords had promising results in predicting the increase or decrease of epidemiological indicators, although it was not able to predict their exact values. Thus, we found that Google Trends data may be useful for predicting outbreaks of coronavirus a few weeks before they happen, and may be used as an auxiliary tool in monitoring and forecasting the disease in Brazil.
4.
Persistent symptoms and decreased health-related quality of life after symptomatic pediatric COVID-19: A prospective study in a Latin American tertiary hospital
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Fink, Thais T.
; Marques, Heloisa H.S.
; Gualano, Bruno
; Lindoso, Livia
; Bain, Vera
; Astley, Camilla
; Martins, Fernanda
; Matheus, Denise
; Matsuo, Olivia M.
; Suguita, Priscila
; Trindade, Vitor
; Paula, Camila S.Y.
; Farhat, Sylvia C.L.
; Palmeira, Patricia
; Leal, Gabriela N.
; Suzuki, Lisa
; Odone Filho, Vicente
; Carneiro-Sampaio, Magda
; Duarte, Alberto José S.
; Antonangelo, Leila
; Batisttella, Linamara R.
; Polanczyk, Guilherme V.
; Pereira, Rosa Maria R.
; Carvalho, Carlos Roberto R.
; Buchpiguel, Carlos A.
; Latronico, Ana Claudia
; Seelaender, Marilia
; Silva, Clovis Artur
; Pereira, Maria Fernanda B.
; Sallum, Adriana M. E.
; Brentani, Alexandra V. M.
; Neto, Álvaro José S.
; Ihara, Amanda
; Santos, Andrea R.
; Canton, Ana Pinheiro M.
; Watanabe, Andreia
; Santos, Angélica C. dos
; Pastorino, Antonio C.
; Franco, Bernadette D. G. M.
; Caruzo, Bruna
; Ceneviva, Carina
; Martins, Carolina C. M. F.
; Prado, Danilo
; Abellan, Deipara M.
; Benatti, Fabiana B.
; Smaria, Fabiana
; Gonçalves, Fernanda T.
; Penteado, Fernando D.
; Castro, Gabriela S. F. de
; Gonçalves, Guilherme S.
; Roschel, Hamilton
; Disi, Ilana R.
; Marques, Isabela G.
; Castro, Inar A.
; Buscatti, Izabel M.
; Faiad, Jaline Z.
; Fiamoncini, Jarlei
; Rodrigues, Joaquim C.
; Carneiro, Jorge D. A.
; Paz, Jose A.
; Ferreira, Juliana C.
; Ferreira, Juliana C. O.
; Silva, Katia R.
; Bastos, Karina L. M.
; Kozu, Katia
; Cristofani, Lilian M.
; Souza, Lucas V. B.
; Campos, Lucia M. A.
; Silva Filho, Luiz Vicente R. F.
; Sapienza, Marcelo T.
; Lima, Marcos S.
; Garanito, Marlene P.
; Santos, Márcia F. A.
; Dorna, Mayra B.
; Aikawa, Nadia E.
; Litvinov, Nadia
; Sakita, Neusa K.
; Gaiolla, Paula V. V.
; Pasqualucci, Paula
; Toma, Ricardo K.
; Correa-Silva, Simone
; Sieczkowska, Sofia M.
; Imamura, Marta
; Forsait, Silvana
; Santos, Vera A.
; Zheng, Yingying
.
OBJECTIVES: To prospectively evaluate demographic, anthropometric and health-related quality of life (HRQoL) in pediatric patients with laboratory-confirmed coronavirus disease 2019 (COVID-19) METHODS: This was a longitudinal observational study of surviving pediatric post-COVID-19 patients (n=53) and pediatric subjects without laboratory-confirmed COVID-19 included as controls (n=52) was performed. RESULTS: The median duration between COVID-19 diagnosis (n=53) and follow-up was 4.4 months (0.8-10.7). Twenty-three of 53 (43%) patients reported at least one persistent symptom at the longitudinal follow-up visit and 12/53 (23%) had long COVID-19, with at least one symptom lasting for >12 weeks. The most frequently reported symptoms at the longitudinal follow-up visit were headache (19%), severe recurrent headache (9%), tiredness (9%), dyspnea (8%), and concentration difficulty (4%). At the longitudinal follow-up visit, the frequencies of anemia (11% versus 0%, p=0.030), lymphopenia (42% versus 18%, p=0.020), C-reactive protein level of >30 mg/L (35% versus 0%, p=0.0001), and D-dimer level of >1000 ng/mL (43% versus 6%, p=0.0004) significantly reduced compared with baseline values. Chest X-ray abnormalities (11% versus 2%, p=0.178) and cardiac alterations on echocardiogram (33% versus 22%, p=0.462) were similar at both visits. Comparison of characteristic data between patients with COVID-19 at the longitudinal follow-up visit and controls showed similar age (p=0.962), proportion of male sex (p=0.907), ethnicity (p=0.566), family minimum monthly wage (p=0.664), body mass index (p=0.601), and pediatric pre-existing chronic conditions (p=1.000). The Pediatric Quality of Live Inventory 4.0 scores, median physical score (69 [0-100] versus 81 [34-100], p=0.012), and school score (60 [15-100] versus 70 [15-95], p=0.028) were significantly lower in pediatric patients with COVID-19 at the longitudinal follow-up visit than in controls. CONCLUSIONS: Pediatric patients with COVID-19 showed a longitudinal impact on HRQoL parameters, particularly in physical/school domains, reinforcing the need for a prospective multidisciplinary approach for these patients. These data highlight the importance of closer monitoring of children and adolescents by the clinical team after COVID-19.
5.
Growth-promoting potential of bacterial biomass in the banana micropropagated plants
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Silva, Christiana de F. B. da
; Brito, Thaís L. de
; Taniguchi, Carlos A. K.
; Lopes, Larissa A.
; Pinto, Gustavo A. S.
; Carvalho, Ana C. P. P. de
.
Revista Brasileira de Engenharia Agrícola e Ambiental
- Journal Metrics
RESUMO Na bananicultura, uma alternativa sustentável para produção de mudas de qualidade seria a inoculação com as bactérias promotoras de crescimento de plantas (BPCP). Portanto, objetivou-se avaliar o potencial da biomassa bacteriana promotora de crescimento em mudas micropropagadas de bananeira cultivar Prata Catarina e identificar quais os mecanismos envolvidos nas interações planta/microrganismo. As provas bioquímicas testadas in vitro foram solubilização de fosfatos, produção de enzimas, produção de amônia, sideróforos e ácido indolacético. Nos ensaios in vivo, as plantas foram bacterizadas (109UFC mL-1) em duas fases: na aclimatização e no cultivo em sacos plásticos. O delineamento experimental foi em blocos casualizados, com 9 e 7 repetições, por tratamento, dos quais foram: T1: controle; T2: plantas tratadas com isolado E2 (Bacillus pumilus group); T3: plantas tratadas com isolado RAB9 (B. pumilus), para cada uma das fases. Os isolados bacterianos foram capazes de produzir celulases, amilases, pectinases, lipases, proteases e sideróforos. Foram observadas nas mudas ganhos em altura, comprimento da raiz, massa seca da raiz, diâmetro do pseudocaule e área foliar. Conclui-se que as BPCP são capazes de promover o crescimento de mudas micropropagadas de bananeira, através da produção de enzimas e sideróforos.
ABSTRACT In the banana production system, a sustainable alternative for producing quality plantlets would be inoculation with plant growth-promoting bacteria (PGPB). Therefore, this study aimed to evaluate the growth-promoting potential of a bacterial biomass in micropropagated banana plantlets cultivar Prata Catarina, and to identify the mechanisms involved in plant-microorganism interactions. In vitro, the biochemical assays tested were the solubilisation of phosphates, production of enzymes, production of ammonia, siderophores, and indole acetic acid. In the in vivo tests, the plants were bacterised (109CFU mL-1) in two phases: acclimatisation, and cultivation in plastic bags. The design was a randomised block with 9 and 7 repetitions per treatment, which were: T1: control; T2: plants treated with isolate E2 (Bacillus pumilus group); T3: plants treated with RAB9 isolate (B. pumilus) for each phase. Bacterial isolates were capable of producing cellulases, amylases, pectinases, lipases, proteases, and siderophores. The plants gained in height, root length, root dry mass, pseudostem diameter, and leaf area. It is concluded that the PGPB can promote the growth of micropropagated banana plantlets through the production of enzymes and siderophores.
https://doi.org/10.1590/1807-1929/agriambi.v22n11p782-787
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6.
Are salty liquid food flavorings in vitro antitumor substances?
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CARVALHO, FRANCISCO R.S.
; MOURA, ANTONIO G.
; RODRIGUES, GARDENIA F.
; NUNES, NARCIA M.F.
; LIMA, DAISY J.B.
; PESSOA, CLAUDIA
; COSTA, MARCILIA P.
; FERREIRA, PAULO M.P.
; PERON, ANA PAULA
.
ABSTRACT The objective of this study was to evaluate the antiproliferative, cytotoxic and genotoxic potential of salty liquid synthetic flavorings of Butter, Cheddar Cheese and Onion. The antiproliferative potential (2.9-1500 µg/mL) was assessed by MTT assay after 72h using the human tumor lines SF-295 (glioblastoma), OVCAR-8 (ovarian), HCT-116 (colon) and HL-60 (promyelocytic leukemia) and primary cultures of murine Sarcoma 180 (S180) and peripheral blood mononuclear cells (PBMC). Allium cepa bulbs were exposed to growing respective doses (1 mL and 2 mL). Only Butter and Cheddar flavorings revealed cytotoxic activity on cancer cells, with IC50 values ranging from 125.4 µg/mL (Cheddar - HCT-116) to 402.6 µg/mL (Butter - OVCAR-8). Butter flavoring was the most cytotoxic on PBMC (136.3 µg/mL) and increased cell division rate in relation to the mitotic index but did not cause cellular aberrations. Onion and Cheddar flavorings reduced the mitotic index after 24h and 48h exposure, but only Onion flavoring resulted in cellular aberrations and mitotic spindle abnormalities, such as anaphase and telophase bridges, micronucleated cells, conchicine-metaphases and amplifications. So, Butter, Onion and/or Cheddar flavorings caused significant changes in the division of meristematic cells of A. cepa and presented cytotoxic action even on decontrolled proliferating human tumor cells.
https://doi.org/10.1590/0001-3765201620150553
1749 downloads
7.
Immunomodulatory and toxicological evaluation of the fruit seeds from Platonia insignis, a native species from Brazilian Amazon Rainforest
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Lustosa, Ana Karina M.F.
; Arcanjo, Daniel D.R.
; Ribeiro, Rayra G.
; Rodrigues, Klinger Antonio F.
; Passos, Flávia Franceli B.
; Piauilino, Celyane A.
; Silva-Filho, José Couras
; Araújo, Bruno Q.
; Lima-Neto, José S.
; Costa-Júnior, Joaquim S.
; Carvalho, Fernando Aécio A.
; Citó, Antônia Maria das Graças L.
.
Abstract The “bacuri” (Platonia insignis Mart., Clusiaceae) is a native tropical fruit from the Brazilian Amazon and Northeast Regions. Its seeds are used to treat inflammatory diseases, diarrhea and skin problems in traditional medical practices. Regarding its widespread medicinal uses, it is important to evaluate the biological and toxicological potential of this species. This way, the aim of this study was to investigate the in vitro cytotoxic and immunomodulatory effects of the hexanic extract of P. insignis seeds, as well as its in vivo acute oral toxicity. The biological evaluation was performed by the determination of cytotoxic (MTT and hemolysis assay) and immunomodulatory (phagocytic capacity, lysosomal volume and nitrite production) activities of EHSB in murine peritoneal macrophages. In addition, the oral acute toxicity was evaluated using female Wistar rats treated with EHSB (2.0 g/kg), in accordance with the OECD 423 Guideline. The EHSB showed low toxicity for macrophages in the MTT test (CC50 value: 90.03 µg/ml), as well as for erythrocytes, which caused only 2.5% hemolysis at the highest concentration. A strong immunomodulatory activity was observed by a markedly increase of the NO production, phagocytic ability and lysosomal volume. On the other hand, it was not observed deaths or changes in the clinical and behavioral parameters in the toxicological evaluation. This manner, the present study contributes to the knowledge about the immunomodulatory and toxicological properties of the P. insignis. This may provide perspectives for the evaluation and development of effective and safe phytomedicines created from the Brazilian local biodiversity.
https://doi.org/10.1016/j.bjp.2015.05.014
1430 downloads
8.
Growing knowledge: an overview of Seed Plant diversity in Brazil
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Zappi, Daniela C.
; Filardi, Fabiana L. Ranzato
; Leitman, Paula
; Souza, Vinícius C.
; Walter, Bruno M.T.
; Pirani, José R.
; Morim, Marli P.
; Queiroz, Luciano P.
; Cavalcanti, Taciana B.
; Mansano, Vidal F.
; Forzza, Rafaela C.
; Abreu, Maria C.
; Acevedo-Rodríguez, Pedro
; Agra, Maria F.
; Almeida Jr., Eduardo B.
; Almeida, Gracineide S.S.
; Almeida, Rafael F.
; Alves, Flávio M.
; Alves, Marccus
; Alves-Araujo, Anderson
; Amaral, Maria C.E.
; Amorim, André M.
; Amorim, Bruno
; Andrade, Ivanilza M.
; Andreata, Regina H.P.
; Andrino, Caroline O.
; Anunciação, Elisete A.
; Aona, Lidyanne Y.S.
; Aranguren, Yani
; Aranha Filho, João L.M.
; Araújo, Andrea O.
; Araújo, Ariclenes A.M.
; Araújo, Diogo
; Arbo, María M.
; Assis, Leandro
; Assis, Marta C.
; Assunção, Vivian A.
; Athiê-Souza, Sarah M.
; Azevedo, Cecilia O.
; Baitello, João B.
; Barberena, Felipe F.V.A.
; Barbosa, Maria R.V.
; Barros, Fábio
; Barros, Lucas A.V.
; Barros, Michel J.F.
; Baumgratz, José F.A.
; Bernacci, Luis C.
; Berry, Paul E.
; Bigio, Narcísio C.
; Biral, Leonardo
; Bittrich, Volker
; Borges, Rafael A.X.
; Bortoluzzi, Roseli L.C.
; Bove, Cláudia P.
; Bovini, Massimo G.
; Braga, João M.A.
; Braz, Denise M.
; Bringel Jr., João B.A.
; Bruniera, Carla P.
; Buturi, Camila V.
; Cabral, Elza
; Cabral, Fernanda N.
; Caddah, Mayara K.
; Caires, Claudenir S.
; Calazans, Luana S.B.
; Calió, Maria F.
; Camargo, Rodrigo A.
; Campbell, Lisa
; Canto-Dorow, Thais S.
; Carauta, Jorge P.P.
; Cardiel, José M.
; Cardoso, Domingos B.O.S.
; Cardoso, Leandro J.T.
; Carneiro, Camila R.
; Carneiro, Cláudia E.
; Carneiro-Torres, Daniela S.
; Carrijo, Tatiana T.
; Caruzo, Maria B.R.
; Carvalho, Maria L.S.
; Carvalho-Silva, Micheline
; Castello, Ana C.D.
; Cavalheiro, Larissa
; Cervi, Armando C.
; Chacon, Roberta G.
; Chautems, Alain
; Chiavegatto, Berenice
; Chukr, Nádia S.
; Coelho, Alexa A.O.P.
; Coelho, Marcus A.N.
; Coelho, Rubens L.G.
; Cordeiro, Inês
; Cordula, Elizabeth
; Cornejo, Xavier
; Côrtes, Ana L.A.
; Costa, Andrea F.
; Costa, Fabiane N.
; Costa, Jorge A.S.
; Costa, Leila C.
; Costa-e-Silva, Maria B.
; Costa-Lima, James L.
; Cota, Maria R.C.
; Couto, Ricardo S.
; Daly, Douglas C.
; De Stefano, Rodrigo D.
; De Toni, Karen
; Dematteis, Massimiliano
; Dettke, Greta A.
; Di Maio, Fernando R.
; Dórea, Marcos C.
; Duarte, Marília C.
; Dutilh, Julie H.A.
; Dutra, Valquíria F.
; Echternacht, Lívia
; Eggers, Lilian
; Esteves, Gerleni
; Ezcurra, Cecilia
; Falcão Junior, Marcus J.A.
; Feres, Fabíola
; Fernandes, José M.
; Ferreira, D.M.C.
; Ferreira, Fabrício M.
; Ferreira, Gabriel E.
; Ferreira, Priscila P.A.
; Ferreira, Silvana C.
; Ferrucci, Maria S.
; Fiaschi, Pedro
; Filgueiras, Tarciso S.
; Firens, Marcela
; Flores, Andreia S.
; Forero, Enrique
; Forster, Wellington
; Fortuna-Perez, Ana P.
; Fortunato, Reneé H.
; Fraga, Cléudio N.
; França, Flávio
; Francener, Augusto
; Freitas, Joelcio
; Freitas, Maria F.
; Fritsch, Peter W.
; Furtado, Samyra G.
; Gaglioti, André L.
; Garcia, Flávia C.P.
; Germano Filho, Pedro
; Giacomin, Leandro
; Gil, André S.B.
; Giulietti, Ana M.
; A.P.Godoy, Silvana
; Goldenberg, Renato
; Gomes da Costa, Géssica A.
; Gomes, Mário
; Gomes-Klein, Vera L.
; Gonçalves, Eduardo Gomes
; Graham, Shirley
; Groppo, Milton
; Guedes, Juliana S.
; Guimarães, Leonardo R.S.
; Guimarães, Paulo J.F.
; Guimarães, Elsie F.
; Gutierrez, Raul
; Harley, Raymond
; Hassemer, Gustavo
; Hattori, Eric K.O.
; Hefler, Sonia M.
; Heiden, Gustavo
; Henderson, Andrew
; Hensold, Nancy
; Hiepko, Paul
; Holanda, Ana S.S.
; Iganci, João R.V.
; Imig, Daniela C.
; Indriunas, Alexandre
; Jacques, Eliane L.
; Jardim, Jomar G.
; Kamer, Hiltje M.
; Kameyama, Cíntia
; Kinoshita, Luiza S.
; Kirizawa, Mizué
; Klitgaard, Bente B.
; Koch, Ingrid
; Koschnitzke, Cristiana
; Krauss, Nathália P.
; Kriebel, Ricardo
; Kuntz, Juliana
; Larocca, João
; Leal, Eduardo S.
; Lewis, Gwilym P.
; Lima, Carla T.
; Lima, Haroldo C.
; Lima, Itamar B.
; Lima, Laíce F.G.
; Lima, Laura C.P.
; Lima, Leticia R.
; Lima, Luís F.P.
; Lima, Rita B.
; Lírio, Elton J.
; Liro, Renata M.
; Lleras, Eduardo
; Lobão, Adriana
; Loeuille, Benoit
; Lohmann, Lúcia G.
; Loiola, Maria I.B.
; Lombardi, Julio A.
; Longhi-Wagner, Hilda M.
; Lopes, Rosana C.
; Lorencini, Tiago S.
; Louzada, Rafael B.
; Lovo, Juliana
; Lozano, Eduardo D.
; Lucas, Eve
; Ludtke, Raquel
; Luz, Christian L.
; Maas, Paul
; Machado, Anderson F.P.
; Macias, Leila
; Maciel, Jefferson R.
; Magenta, Mara A.G.
; Mamede, Maria C.H.
; Manoel, Evelin A.
; Marchioretto, Maria S.
; Marques, Juliana S.
; Marquete, Nilda
; Marquete, Ronaldo
; Martinelli, Gustavo
; Martins da Silva, Regina C.V.
; Martins, Ângela B.
; Martins, Erika R.
; Martins, Márcio L.L.
; Martins, Milena V.
; Martins, Renata C.
; Matias, Ligia Q.
; Maya-L., Carlos A.
; Mayo, Simon
; Mazine, Fiorella
; Medeiros, Debora
; Medeiros, Erika S.
; Medeiros, Herison
; Medeiros, João D.
; Meireles, José E.
; Mello-Silva, Renato
; Melo, Aline
; Melo, André L.
; Melo, Efigênia
; Melo, José I.M.
; Menezes, Cristine G.
; Menini Neto, Luiz
; Mentz, Lilian A.
; Mezzonato, A.C.
; Michelangeli, Fabián A.
; Milward-de-Azevedo, Michaele A.
; Miotto, Silvia T.S.
; Miranda, Vitor F.O.
; Mondin, Cláudio A.
; Monge, Marcelo
; Monteiro, Daniele
; Monteiro, Raquel F.
; Moraes, Marta D.
; Moraes, Pedro L.R.
; Mori, Scott A.
; Mota, Aline C.
; Mota, Nara F.O.
; Moura, Tania M.
; Mulgura, Maria
; Nakajima, Jimi N.
; Nardy, Camila
; Nascimento Júnior, José E.
; Noblick, Larry
; Nunes, Teonildes S.
; O'Leary, Nataly
; Oliveira, Arline S.
; Oliveira, Caetano T.
; Oliveira, Juliana A.
; Oliveira, Luciana S.D.
; Oliveira, Maria L.A.A.
; Oliveira, Regina C.
; Oliveira, Renata S.
; Oliveira, Reyjane P.
; Paixão-Souza, Bruno
; Parra, Lara R.
; Pasini, Eduardo
; Pastore, José F.B.
; Pastore, Mayara
; Paula-Souza, Juliana
; Pederneiras, Leandro C.
; Peixoto, Ariane L.
; Pelissari, Gisela
; Pellegrini, Marco O.O.
; Pennington, Toby
; Perdiz, Ricardo O.
; Pereira, Anna C.M.
; Pereira, Maria S.
; Pereira, Rodrigo A.S.
; Pessoa, Clenia
; Pessoa, Edlley M.
; Pessoa, Maria C.R.
; Pinto, Luiz J.S.
; Pinto, Rafael B.
; Pontes, Tiago A.
; Prance, Ghillean T.
; Proença, Carolyn
; Profice, Sheila R.
; Pscheidt, Allan C.
; Queiroz, George A.
; Queiroz, Rubens T.
; Quinet, Alexandre
; Rainer, Heimo
; Ramos, Eliana
; Rando, Juliana G.
; Rapini, Alessandro
; Reginato, Marcelo
; Reis, Ilka P.
; Reis, Priscila A.
; Ribeiro, André R.O.
; Ribeiro, José E.L.S.
; Riina, Ricarda
; Ritter, Mara R.
; Rivadavia, Fernando
; Rocha, Antônio E.S.
; Rocha, Maria J.R.
; Rodrigues, Izabella M.C.
; Rodrigues, Karina F.
; Rodrigues, Rodrigo S.
; Rodrigues, Rodrigo S.
; Rodrigues, Vinícius T.
; Rodrigues, William
; Romaniuc Neto, Sérgio
; Romão, Gerson O.
; Romero, Rosana
; Roque, Nádia
; Rosa, Patrícia
; Rossi, Lúcia
; Sá, Cyl F.C.
; Saavedra, Mariana M.
; Saka, Mariana
; Sakuragui, Cássia M.
; Salas, Roberto M.
; Sales, Margareth F.
; Salimena, Fatima R.G.
; Sampaio, Daniela
; Sancho, Gisela
; Sano, Paulo T.
; Santos, Alessandra
; Santos, Élide P.
; Santos, Juliana S.
; Santos, Marianna R.
; Santos-Gonçalves, Ana P.
; Santos-Silva, Fernanda
; São-Mateus, Wallace
; Saraiva, Deisy P.
; Saridakis, Dennis P.
; Sartori, Ângela L.B.
; Scalon, Viviane R.
; Schneider, Ângelo
; Sebastiani, Renata
; Secco, Ricardo S.
; Senna, Luisa
; Senna-Valle, Luci
; Shirasuna, Regina T.
; Silva Filho, Pedro J.S.
; Silva, Anádria S.
; Silva, Christian
; Silva, Genilson A.R.
; Silva, Gisele O.
; Silva, Márcia C.R.
; Silva, Marcos J.
; Silva, Marcos J.
; Silva, Otávio L.M.
; Silva, Rafaela A.P.
; Silva, Saura R.
; Silva, Tania R.S.
; Silva-Gonçalves, Kelly C.
; Silva-Luz, Cíntia L.
; Simão-Bianchini, Rosângela
; Simões, André O.
; Simpson, Beryl
; Siniscalchi, Carolina M.
; Siqueira Filho, José A.
; Siqueira, Carlos E.
; Siqueira, Josafá C.
; Smith, Nathan P.
; Snak, Cristiane
; Soares Neto, Raimundo L.
; Soares, Kelen P.
; Soares, Marcos V.B.
; Soares, Maria L.
; Soares, Polyana N.
; Sobral, Marcos
; Sodré, Rodolfo C.
; Somner, Genise V.
; Sothers, Cynthia A.
; Sousa, Danilo J.L.
; Souza, Elnatan B.
; Souza, Élvia R.
; Souza, Marcelo
; Souza, Maria L.D.R.
; Souza-Buturi, Fátima O.
; Spina, Andréa P.
; Stapf, María N.S.
; Stefano, Marina V.
; Stehmann, João R.
; Steinmann, Victor
; Takeuchi, Cátia
; Taylor, Charlotte M.
; Taylor, Nigel P.
; Teles, Aristônio M.
; Temponi, Lívia G.
; Terra-Araujo, Mário H.
; Thode, Veronica
; Thomas, W.Wayt
; Tissot-Squalli, Mara L.
; Torke, Benjamin M.
; Torres, Roseli B.
; Tozzi, Ana M.G.A.
; Trad, Rafaela J.
; Trevisan, Rafael
; Trovó, Marcelo
; Valls, José F.M.
; Vaz, Angela M.S.F.
; Versieux, Leonardo
; Viana, Pedro L.
; Vianna Filho, Marcelo D.M.
; Vieira, Ana O.S.
; Vieira, Diego D.
; Vignoli-Silva, Márcia
; Vilar, Thaisa
; Vinhos, Franklin
; Wallnöfer, Bruno
; Wanderley, Maria G.L.
; Wasshausen, Dieter
; Watanabe, Maurício T.C.
; Weigend, Maximilian
; Welker, Cassiano A.D.
; Woodgyer, Elizabeth
; Xifreda, Cecilia C.
; Yamamoto, Kikyo
; Zanin, Ana
; Zenni, Rafael D.
; Zickel, Carmem S
.
Resumo Um levantamento atualizado das plantas com sementes e análises relevantes acerca desta biodiversidade são apresentados. Este trabalho se iniciou em 2010 com a publicação do Catálogo de Plantas e Fungos e, desde então vem sendo atualizado por mais de 430 especialistas trabalhando online. O Brasil abriga atualmente 32.086 espécies nativas de Angiospermas e 23 espécies nativas de Gimnospermas e estes novos dados mostram um aumento de 3% da riqueza em relação a 2010. A Amazônia é o Domínio Fitogeográfico com o maior número de espécies de Gimnospermas, enquanto que a Floresta Atlântica possui a maior riqueza de Angiospermas. Houve um crescimento considerável no número de espécies e nas taxas de endemismo para a maioria dos Domínios (Caatinga, Cerrado, Floresta Atlântica, Pampa e Pantanal), com exceção da Amazônia que apresentou uma diminuição de 2,5% de endemicidade. Entretanto, a maior parte das plantas com sementes que ocorrem no Brasil (57,4%) é endêmica deste território. A proporção de formas de vida varia de acordo com os diferentes Domínios: árvores são mais expressivas na Amazônia e Floresta Atlântica do que nos outros biomas, ervas são dominantes no Pampa e as lianas apresentam riqueza expressiva na Amazônia, Floresta Atlântica e Pantanal. Este trabalho não só quantifica a biodiversidade brasileira, mas também indica as lacunas de conhecimento e o desafio a ser enfrentado para a conservação desta flora.
Abstract An updated inventory of Brazilian seed plants is presented and offers important insights into the country's biodiversity. This work started in 2010, with the publication of the Plants and Fungi Catalogue, and has been updated since by more than 430 specialists working online. Brazil is home to 32,086 native Angiosperms and 23 native Gymnosperms, showing an increase of 3% in its species richness in relation to 2010. The Amazon Rainforest is the richest Brazilian biome for Gymnosperms, while the Atlantic Rainforest is the richest one for Angiosperms. There was a considerable increment in the number of species and endemism rates for biomes, except for the Amazon that showed a decrease of 2.5% of recorded endemics. However, well over half of Brazillian seed plant species (57.4%) is endemic to this territory. The proportion of life-forms varies among different biomes: trees are more expressive in the Amazon and Atlantic Rainforest biomes while herbs predominate in the Pampa, and lianas are more expressive in the Amazon, Atlantic Rainforest, and Pantanal. This compilation serves not only to quantify Brazilian biodiversity, but also to highlight areas where there information is lacking and to provide a framework for the challenge faced in conserving Brazil's unique and diverse flora.
https://doi.org/10.1590/2175-7860201566411
33340 downloads
9.
A psicologia como neurociência cognitiva: Implicações para a compreensão dos processos básicos e suas aplicações
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Gonçalves, Óscar F.
; Sampaio, Adriana
; Mesquita, Ana
; Petrosyan, Avagni
; Pinheiro, Ana P.
; Carvalho, Sandra
; Leite, Jorge
; Coutinho, Joana
; Osório, Ana
; Oliveira-Silva, Patrícia
.
O presente artigo procura ilustrar o modo como os desenvolvimentos das neurociências cognitivas poderão ajudar a compreender alguns dos processos psicológicos básicos e, simultaneamente, ser traduzidos para importantes domínios da psicologia aplicada, particularmente no domínio clínico. Exemplificaremos a partir de algumas linhas de investigação programática em curso nas diferentes subsecções do Laboratório de Neuropsicofisiologia da Escola de Psicologia da Universidade do Minho. As potencialidades metodológicas proporcionadas pela neurofisiologia, neuroimagiologia, neuromodelação, psicofisiologia, neurobioquímica e neurogenética serão exemplificadas nas suas aplicações à linguagem (e suas implicações para a compreensão da esquizofrenia), funcionamento sócio-cognitivo (e implicações para a compreensão das perturbações do neurodesenvolvimento), funcionamento executivo (com implicações para a compreensão das perturbações do espectro obsessivo), empatia (e implicações para a compreensão da psicoterapia), mecanismos de stress (com implicações para a compreensão das perturbações de ansiedade), e, finalmente, comportamento animal (com implicações para o conhecimento dos sistemas sensoriais e perceptuais).
The present article illustrates how developments in the cognitive neurosciences may contribute to the understanding of basic psychological processes and, simultaneously, translating this understanding to applied psychology, particularly in the clinical domain. We will illustrate with examples of current programmatic lines being carried out in the different subsections of the Neuropsychophysiology Lab at the School of Psychology at the University of Minho. The methodological potentialities available in neurophysiology, neuroimagiology, neuromodulation, psychophysiology, neurobiochemistry and neurogenetics will be exemplified in their applications to language (and the understanding of schizophrenia), social-cognitive functioning (and the understanding of neurodevelopmental disorders), executive functioning (and obsessive spectrum disorders), empathy (and its implications for psychotherapy), stress mechanisms (implications for the understanding of anxiety disorders) and, finally, animal.
https://doi.org/10.14417/ap.836
19242 downloads
10.
Concentração de nitrato no perfil do solo fertigado com diferentes concentrações de fontes nitrogenadas
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Revista Brasileira de Engenharia Agrícola e Ambiental
- Journal Metrics
Objetivou-se, neste trabalho, avaliar o efeito da concentração de nitrato de potássio e ureia da água de irrigação no teor de nitrato da solução do solo e do extrato de saturação, em duas profundidades do sistema radicular da bananeira tipo Plátano, cultivar Terra Maranhão fertigada. O experimento conduzido entre janeiro e dezembro de 2008 seguiu um delineamento em blocos casualizados com três repetições em esquema fatorial 2 x 4, em que os tratamentos consistiram no uso de duas fontes de nitrogênio (ureia e nitrato de potássio) sob quatro concentrações (2,7; 4,0; 6,5 e 7,2 g L-1), aplicadas via gotejamento, com três emissores de 4 L h-1 por planta. Amostras de solo e de solução do solo foram colhidas mensalmente em todos os tratamentos nas profundidades de 0,30 e 0,60 m, com determinação em laboratório da concentração de nitrato na solução do solo e no extrato de saturação. Os resultados permitiram concluir que o aumento da concentração do fertilizante nitrogenado da água na fertigação implicou em aumento da concentração do íon nitrato na solução do solo e no extrato de saturação para as duas fontes de nitrogênio aplicadas.
The objective of this study was to evaluate the effects of concentration of urea and potassium nitrate in the irrigation water on the concentration of nitrate in the soil solution and in saturation extract of soil at two depths of root system of Plaintans banana cv Terra Maranhão under fertigation. The experiment was carried out during the period of January to December, 2008 and followed a randomized block design with three replications in a 2 x 4 factorial scheme where treatments consisted of the use of two nitrogen sources (urea and potassium nitrate) under four concentrations (2.7; 4.0; 6.5 and 7.2 g L-1), applied by drip irrigation with three 4 L h-1 emitter per plant. Soil and soil solution samples were collected monthly in all treatments at depths of 0.30 and 0.60 m and nitrate concentration was determined in the soil solution and saturation extract. Results allowed to conclude that the increase of nitrogen fertilizer concentration in fertigation water caused an increase of nitrate ion concentration in the soil solution and in saturation extract for the two applied sources.
3601 downloads
11.
Análise microscópica e ultraestrutural das glândulas salivares mandibulares de Procyon cancrivorus
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Santos, Amilton C.
; Oliveira, Vanessa C.
; Viana, Diego C.
; Lobo, Luis M.
; Ambrósio, Carlos E.
; Assis-Neto, Antônio C.
; Carvalho, Ana F.
; Mançanares, Celina A.F.
.
Devido a importância ecológica dos Procyon cancrivorus, o objetivo deste trabalho foi caracterizar morfologicamente e ultra estruturalmente a glândula salivar mandibular desta espécie. Foram utilizadas 10 pares (direita e esquerda) de glândulas salivares mandibulares de cinco animais adultos. As glândulas salivares mandibulares foram dissecadas e mensuradas com paquímetro de precisão e posteriormente processadas por técnica rotineira de histologia e coradas por HE (hematoxilina e eosina) e Picrossírius. Fragmentos das glândulas foram processados para análise em microscopia eletrônica de varredura (MEV). As glândulas salivares mandibulares direitas e esquerdas de P. cancrivorus apresentaram-se lobuladas, formato ovalado, e posicionadas entre a fossa atlantis e o osso basihyoideum do crânio. Microscopicamente, estas glândulas estavam revestidas por uma cápsula de tecido conjuntivo denso não modelado, a qual adentra a glândula através de septos, dividindo-a em lóbulos. Nos septos de tecido conjuntivo estão presentes vasos sanguíneos e nervos, além de ductos interlobulares excretores. Dentro dos lóbulos das glândulas salivares mandibulares, são encontrados ductos do tipo estriado e intercalar, além de predomínio de ácinos do tipo mucoso. Os resultados permitem concluir que as glândulas salivares mandibulares dos Procyon cancrivorus, seguem o padrão estrutural descrito em outras espécies de mamíferos. No entanto os tipos de ácinos podem variar entre as espécies, sendo, portanto, necessários futuros estudos histoquímicos e de biologia celular para desvendar a importância dessa variação para a espécie aqui estudada, comparando com seu hábito alimentar.
Due to the ecological importance of Procyon cancrivorus, the aim of this study was to characterize morphologically and ultrastructurally the mandibular salivary gland of this specie. We used 10 pairs (right and left) of mandibular salivary glands from five adult animals. The mandibular salivary glands were dissected and measured by precision calipers and later processed by histological routine technique, stained by HE (hematoxylin and eosin) and Picrossirius. Fragments of the glands were processed for analysis by scanning electron microscopy (SEM). The mandibular salivary gland, right and left from P. cancrivorus presents lobulated and oval-shape, and it was positioned between the fossa atlantis and basihyoideum ossis of skull. Microscopically, these glands are lined by a capsule formed by dense irregular connective tissue, which enters into the gland by septa, dividing it into lobules. Into the septa of connective tissue were present blood vessels and nerves, and interlobular excretory ducts. Inside the lobes of the mandibular salivary glands, striatum and intermediate-type ducts are found, and the predominance of mucous acini was observed. The results indicate that the mandibular salivary glands of Procyon cancrivorus, follows the structural pattern described in other mammalian species. However the types of acini may vary among species, and thus further histochemical and cell biology studies are required in order to uncover the significance of this variation for the specie here studied, according with their feeding habit.
4777 downloads
12.
Antiproliferative activity of methanol extracts of four species of Croton on different human cell lines
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Savietto, Jóice P.
; Furlan, Cláudia M.
; Motta, Lucimar B.
; Salatino, Maria Luiza F.
; Carvalho, João E.
; Ruiz, Ana Lucia T. G.
; Salatino, Antonio
; Santos, Déborah Yara A. C.
.
Several species of Croton have been described with biological activities, mainly due to diterpenes, alkaloids and/or other secondary metabolites. These activities account for the traditional use of Croton species to treat certain diseases in South America, Asia and Western Africa. The crude methanol extracts obtained from leaves and steam bark of Croton dichrous Müll. Arg., C. erythroxyloides Baill., C. myrianthus Müll. Arg. and C. splendidus Mart. ex Colla were tested for antiproliferative activity against ten human cancer cell lines. Chemical analyses of all extracts were carried out by GC/MS and HPLC/MS/MS. The leaf extract obtained from C. erythroxyloides showed potent activity against PC-3 (prostate) and OVCAR-3 (ovary) cell lines. Lupeol is suggested to be involved in such activity. Tiliroside, an acyl-glycosilated flavonoid ubiquitous in all tested extracts, seems to play an important role in the observed moderate activity of most extracts against the leukemia K562 cell lineage.
2353 downloads
13.
Therapeutic dose of Ginkgo biloba extract 761 may alter the urine excretion of Wistar rats
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Dalmacio, Monica S. C.
; Campos, Sylvia M. N.
; Carvalho, Jorge J. J.
; Pereira, Mário J. S.
; Nascimento, Ana Lúcia
; Paschoal, Patricia O.
; Sobrinho, Andrea P.
; Pedruzzi, Monique M. B.
; Garrido, Valeria
; Moreno, Silvana R. F.
; Teixeira, Gerlinde A. P. B.
; Cardoso, Gilberto P.
.
Wistar rats (n=20) were divided in two groups: G1 received 2 mg/kg of GBE (Ginkgo biloba extract 761), whereas G2 received the same volume of a sodium chloride solution (0.9%), both for 10 days. After a 7-day interval, the treatment was repeated for 8 days. Urine volume and food and water intake were measured daily during this protocol. Histological assessments were performed. No significant difference (p>0.05) was observed in food and water intake of animals during treatment with GBE. Animals who received GBE had a smaller urine volume and increase of weight with a significance difference (p<0.05) during the first and second exposure period. No histological alteration was observed in tissues, except for the kidney of the experimental group, which revealed a higher concentration of red cells in the glomerulus with a strong staining for Vascular Endothelial Growth Factor (VEGF). The introduction of GBE (therapeutic dose) in health rats may promote alterations in the physiology of the kidney, but no sufficient to modify the glomerulus architecture, including at ultra structural level (electron microscopy).
3778 downloads
14.
Testing simple criteria for de age estimation of six hunted mammal species in Brazilian amazon
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Mendes-Oliveira, Ana Cristina
; de Maria, Susanne L. S
; Soares de Lima, Renata Cecília
; Fernandes, Alexandre S
; de Almeida, Paula C. R
; Montag, Luciano F. A
; de Carvalho Jr, Oswaldo
.
Muitos métodos para estimativa de idade de mamíferos têm sido utilizados ultimamente. Entretanto, a maioria deles requerem procedimentos laboratoriais, que geralmente, não estão disponíveis em campo. Neste artigo nós definimos categorias de idade para seis espécies de mamíferos através da análise de desgaste dentário, erupção dentária e massa corporal. Nós analisamos 632 crânios, incluindo Mazama nemorivaga (veado cinza), Mazama americana (veado vermelhor), Tayassu pecari (queixada), Pecari tajacu (cateto), Cuniculus paca (paca) and Dasyprocta leporina (cotia). Usando critérios biológicos e morfológicos nós definimos quatro categorias de idade para cada espécie: jovem, sub-adulto, adulto e adulto senescente. Foram definidos padrões de desgaste e erupção dos molares para cada categoria de idade para cada uma das espécies analisadas. Nós encontramos diferenças significativas das médias de peso entre as categorias de idade para as espécies D. leporina (F = 81.1; df = 3; p < 0.05), C. paca (F = 39.0; df = 3; p < 0.05), P. tajacu (F = 24.6; df = 3; p < 0.05) and M. americana (F = 30.6; df = 3; p < 0.05), sugerindo que a massa corporal pode ser usada como um critério complementar de estimativa de idade. Embora seja um método menos quantitativo do que aqueles citados com mais frequência na literatura, como deposição de bandas de cementum, o método descrito neste artigo mostrou ser uma ferramenta eficiente para pesquisadores e manejadores em trabalhos de campo na Amazônia brasileira. Embora métodos similares já tenham sido utilizados em estudos de caça, é a primeira vez que esta metodologia é descrita em detalhes, podendo ser replicada em outros estudos semelhantes.
Many methods of age determination have been used to date. However, most of them require laboratory procedures which are, in general, not available for wildlife managers in the field. In this paper we attempt to standardize the age categories for six mammal species through the analysis of dental wear, dental eruption and body mass. We analyzed 632 skulls, including Mazama nemorivaga (brown brocket deer), Mazama americana (red brocket deer), Tayassu pecari (white-lipped peccary), Pecari tajacu (collared peccary), Cuniculus paca (paca) and Dasyprocta leporina (agouti). Using biological and morphological criteria we define four age categories: young, sub adult, adult and senescent adult. Scores and variables of molar cusp wear and eruption were defined for each age category within each species. We found significant differences in mean weights among age categories for D. leporina (F = 81.1; df = 3; p < 0.05), C. paca (F = 39.0; df = 3; p < 0.05), P. tajacu (F = 24.6; df = 3; p < 0.05) and M. americana (F = 30.6; df = 3; p < 0.05), suggesting that body mass can be a complementary criterion for estimating age. Despite being less quantitative than methods such as those which use deposition of cementum layers, the method described here proved to be an efficient tool for field researchers and managers in the Brazilian Amazon. Although similar methods have already been applied in the study of hunted mammals in the Peruvian and Brazilian Amazon, it is the first time such a method is described in detail and therefore it can be easily used in future works.
450 downloads
15.
Avaliação da eficácia de diferentes protocolos de preparo do Plasma Rico em Plaquetas para uso em Medicina Equina
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Vendruscolo, Cynthia P.
; Carvalho, Armando de M
; Moraes, Lívia F.
; Maia, Leandro
; Queiroz, Diana L
; Watanabe, Marcos J
; Yamada, Ana Lúcia M.
; Alves, Ana Liz G
.
O Plasma Rico em Plaquetas (PRP) é um preparado do sangue total que contém diversos fatores de crescimento responsáveis pela proliferação e diferenciação celular, angiogênese, como também pelo aumento da produção da matriz extracelular. Nesse sentido, o objetivo do presente estudo foi testar 10 protocolos diferentes de centrifugação para obtenção de PRP a partir do sangue total de equinos hígidos. Para isso foram utilizadas 10 amostras de 27mL de sangue total de cinco animais, as quais foram centrifugadas conforme cada protocolo proposto. Os resultados revelaram que os protocolos com menor força de centrifugação relativa resultaram em maior (p<0,05) concentração de plaquetas e, que não houve (p>0,05) influência do tempo de centrifugação em relação a essa variável. A influência do tempo foi observada apenas no número de leucócitos em protocolos com menor força de centrifugação relativa (FCR). Os quatro melhores protocolos, que obtiveram as maiores concentrações de plaquetas, foram submetidos à análise pelo teste de ELISA para dosar a quantidade de TGF-β que não revelou diferença (p>0,05) entre os protocolos.
Platelet-rich plasma (PRP) is a prepared of the whole blood which contains several growth factors responsible for cellular proliferation and differentiation, angiogenesis, and for the increase of the extracellular matrix. Thus, the aim of this study was to test 10 different centrifugation protocols to obtain PRP from the whole blood of healthy equines. Ten samples of 27mL of the whole blood of 5 healthy equines were used, which were centrifuged in accordance to the proposed protocols. The results showed that the protocols with less relative centrifugation force resulted in greater (p<0,05) platelets concentration. Also, platelets concentration was not influenced by varying the time of centrifugation. However, time did affect the number of leukocytes in some protocols. The best four protocols were analyzed by ELISA test to quantitate the amount of TGF-β, which revealed no difference (p> 0.05) between the protocols.
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subject | subject (title words, abstract and keywords) |
ab | abstract |
ta | journal short title (e.g. Cad. Saúde Pública) |
journal_title | journal full title (e.g. Cadernos de Saúde Pública) |
la | publication language code (e.g. pt - Portuguese, es - Spanish) |
type | document type |
pid | publication identifier |
publication_year | publication year of publication |
sponsor | sponsor |
aff_country | country code of the author's affiliation |
aff_institution | author affiliation institution |
volume | article volume |
issue | article issue |
elocation | elocation |
doi | DOI number |
issn | journal ISSN |
in | SciELO colection code (e.g. scl - Brasil, col - Colômbia) |
use_license | article usage license code |