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au:CARVALHO, JAIRO A. DE

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The alien species Stenochrus portoricensis (Schizomida: Hubbardiidae): decreasing the Wallacean shortfall in the New World
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Villarreal, Osvaldo

; Sánchez, Nestor

; Ascenção, Antonio de

; Carvalho, Leonardo S.

; Delgado-Santa, Leonardo

; Moreno-González, Jairo A.

Iheringia. Série Zoologia

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2023, Volume 113 elocation e2023005

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT The widely distributed species, Stenochrus portoricensis Chamberlin, 1922, is recorded for the first time from Costa Rica and Venezuela, and new occurrences from Brazil and Colombia are presented. Morphology of spermathecae from 14 localities is compared and illustrated. The need for studies to evaluate the potential status as an invasive species of S. portoricensis is commented on and a distribution map of the species in southern Central America and South America is given.

RESUMEN La especie exótica Stenochrus portoricensis Chamberlin, 1922 (Schizomida: Hubbardiidae): disminución del déficit de Wallace en el Nuevo Mundo. La especie de amplia distribución Stenochrus portoricensis Chamberlin, 1922 se registra por primera vez en Costa Rica y Venezuela, y se presentan nuevas ocurrencias en Brasil y Colombia. Se compara e ilustra la morfología de las espermatecas de especímenes provenientes de 14 localidades. Se comenta la necesidad de realizar estudios para evaluar el estatus potencial de S. portoricensis como especie invasora y se presenta un mapa de distribución de la especie en el sur de Centroamérica y Sudamérica.

https://doi.org/10.1590/1678-4766e2023005

Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen
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Tirapelli, Daniela P. C

; Cirino, Mucio Luiz de Assis

; Carvalho, Camila A. Melo de

; Novais, Paulo Cezar

; Figueredo, Rafael Zimak

; Porsani, Lucas Barbosa

; Gula, Isabella Stracieri

; Rodrigues, Andressa Romualdo

; Silva, Jairo Pinheiro da

; Pereira, Adriana Lis

; Lizarte Neto, Fermino Sanches

; Schimming, Bruno Cesar

; Tazima, Maria de Fátima Galli Sorita

; Fazan, Valéria de Paula Sassoli

; Carlotti-Jr, Carlos Gilberto

; Tirapelli, Luis Fernando

; Colli, Benedicto Oscar

International Journal of Morphology

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jun 2020, Volume 38 N. 3 Pages 523 - 529

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

SUMMARY: This study aimed to investigate the morphometric and the pattern of protein and gene expression related to the extrinsic apoptotic pathway in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. For this analysis, 120 rats were randomly divided into 3 groups (20 animals each): control - no surgery (20 animals); sham - simulation of surgery (20 animals); ischemic - focal ischemia for 1 hour, without reperfusion (80 animals) and divided into four subgroups with 20 animals each: ischemic + intraischemic hypothermia; ischemic + previous intravenous ketoprofen, and ischemic + hypothermia and ketoprofen. The infarct volume was measured using morphometric analysis of infarct areas defined by triphenyl tetrazolium chloride and the patterns of expression of the apoptosis genes (Fas, c-Flip, caspase-8 and caspase-3) and the apoptosis protein caspase-3 were evaluated by quantitative real-time PCR and immunohistochemistry, respectively. Hypo expression of genes of extrinsic pathway of apoptosis was observed: Fas receptor, c-Flip and caspase-8 in the ischemics areas. Increases in the gene and protein caspase-3 in the ischemic areas were also observed, and these increases were reduced by hypothermia and ketoprofen, also noted in the morphometric study. The caspases-3 increase suggests that this gene plays an important role in apoptosis, probably culminating in cell death and that the neuroprotective effect of hypothermia and ketoprofen is involved.

RESUMEN: Este estudio tuvo como objetivo investigar la morfometría y el patrón de expresión de proteínas y genes relacionados con la vía apoptótica extrínseca en la isquemia cerebral focal experimental y el agujero de neuroprotección con hipotermia y ketoprofeno. Se dividieron aleatoriamente 120 ratas en 3 grupos (20 animales cada uno): control - sin cirugía (20 animales); simulación - simulación de cirugía (20 animales); isquemia isquemia focal durante 1 hora, sin reperfusión (80 animales) y dividida en cuatro subgrupos con 20 animales cada uno: isquemia + hipotermia intraisquémica; isquemia + ketoprofeno intravenoso previo, e isquemia + hipotermia y ketoprofeno. El volumen del infarto se midió utilizando un análisis morfométrico de áreas de infarto definidas por cloruro de trifenil tetrazolio y los patrones de expresión de los genes de apoptosis (Fas, c-Flip, caspase-8 y caspase-3) y la proteína de apoptosis caspase-3 fueron evaluados por PCR cuantitativa en tiempo real e inmunohistoquímica, respectivamente. Se observó hipoexpresión de genes de la vía extrínseca de la apoptosis: receptor Fas, c-Flip y caspasa-8 en las áreas isquémicas. También se observaron aumentos en el gen y la proteína caspasa-3 en las áreas isquémicas y estos aumentos se redujeron por hipotermia y ketoprofeno, también observado por estudio morfométrico. El aumento de caspasas-3 sugiere que este gen tiene un papel importante en la apoptosis, y probable causa de muerte celular, involucrando el efecto neuroprotector de la hipotermia y el ketoprofeno.

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Analysis of Caspase-9 protein and microRNAs miR-21, miR-126 and miR-155 related to the apoptosis mechanism in the cerebellum of rats submitted to focal cerebral ischemia associated with an alcoholism model
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SILVA, Jairo Pinheiro da

; LIZARTE NETO, Fermino Sanches

; CIRINO, Mucio Luiz de Assis

; CARVALHO, Camila Albuquerque Melo de

; CARLOTTI JR, Carlos Gilberto

; COLLI, Benedicto Oscar

; TIRAPELLI, Daniela Pretti da Cunha

; TIRAPELLI, Luís Fernando

Arquivos de Neuro-Psiquiatria

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oct 2019, Volume 77 N. 10 Pages 689 - 695

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O objetivo deste estudo foi analisar o cerebelo de ratos submetidos à isquemia cerebral focal experimental, por oclusão da artéria cerebral média por 90 minutos, seguida de reperfusão por 48 horas, associada a um modelo de alcoolismo. Métodos Foram utilizados 50 ratos Wistar adultos, subdivididos em cinco grupos experimentais: grupo controle (C): animais submetidos apenas à anestesia; grupo sham (S): animais submetidos à simulação completa do procedimento cirúrgico; grupo isquêmico (I): animais submetidos à isquemia cerebral focal por 90 minutos, seguidos de reperfusão por 48 horas; grupo alcoólico (A): animais que receberam etanol absoluto diário diluído em 20% em água por quatro semanas; e grupo isquêmico e alcoólico (I + A): animais que recebem o mesmo tratamento do grupo A e, após quatro semanas, submetidos à isquemia cerebral focal por 90 minutos, seguidos de reperfusão por 48 horas. As amostras de cerebelo foram coletadas e a análise imuno-histoquímica da proteína Caspase-9 e a análise sérica por RT-PCR dos microRNAs miR-21, miR-126 e miR155 foram realizadas. Resultados A expressão de Caspase-9 foi maior nos grupos I, A e I + A. Nas análises de microRNAs, o miR-126 foi maior nos grupos A e I + A, o miR-155 foi maior nos grupos I e I + A. Conclusões Concluímos que a apoptose ocorre no córtex cerebelar, mesmo distante do foco isquêmico, e que os microRNAs 126 e 155 mostram uma correlação com a apoptose celular em ratos isquêmicos e submetidos ao modelo crônico de álcool.

ABSTRACT This study aimed to analyze the cerebellum of rats submitted to an experimental focal cerebral ischemia, by middle cerebral artery occlusion for 90 minutes, followed by reperfusion for 48 hours, associated with an alcoholism model. Methods Fifty adult Wistar rats were used, subdivided into five experimental groups: control group (C): animals submitted to anesthesia only; sham group (S): animals submitted to complete simulation of the surgical procedure; ischemic group (I): animals submitted to focal cerebral ischemia for 90 minutes followed by reperfusion for 48 hours; alcoholic group (A): animals that received daily absolute ethanol diluted 20% in water for four weeks; and, ischemic and alcoholic group (I + A): animals receiving the same treatment as group A and, after four weeks, submitted to focal cerebral ischemia for 90 minutes, followed by reperfusion for 48 hours. The cerebellum samples were collected and immunohistochemical analysis of Caspase-9 protein and serum analysis by RT-PCR of microRNAs miR-21, miR-126 and miR155 were performed. Results The expression of Caspase-9 was higher in groups I, A and I + A. In the microRNAs analyses, miR-126 was higher in groups A and I + A, miR-155 was higher in groups I and I + A. Conclusions We conclude that apoptosis occurs in the cerebellar cortex, even if it is distant from the ischemic focus, and that microRNAs 126 and 155 show a correlation with cellular apoptosis in ischemic rats and those submitted to the chronic alcohol model.

https://doi.org/10.1590/0004-282x20190126 1287 downloads

Mercury content in the fur of jaguars (Panthera onca) from two areas under different levels of gold mining impact in the Brazilian Pantanal
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MAY JÚNIOR, JOARES A.

; QUIGLEY, HOWARD

; HOOGESTEIJN, RAFAEL

; TORTATO, FERNANDO R.

; DEVLIN, ALLISON

; CARVALHO JÚNIOR, RUI M. DE

; MORATO, RONALDO G.

; SARTORELLO, LEONARDO R.

; RAMPIM, LILIAN E.

; HABERFELD, MARIO

; PAULA, ROGÉRIO C. DE

; ZOCCHE, JAIRO JOSÉ

Anais da Academia Brasileira de Ciências

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aug 2018, Volume 90 N. 2 Pages 2129 - 2139

Abstract: En | Text: En | PDF: En

ABSTRACT The Pantanal is the largest inland wetland in the world and is under increasing anthropogenic threats, including long-term regionally intensive gold mining practices. Gold mining activities are known to cause the release of harmful pollutants such as mercury (Hg) to the surrounding environment. Jaguars (Panthera onca (Linnaeus, 1758)) are apex predators, and therefore show great potential to accumulate Hg by biomagnification. We hypothesize that total Hg content in the fur of jaguars from two sites within the Brazilian Pantanal would be significantly different as a function of distance from active gold mining operations. The Hg content was determined by fluorescence spectrometry. The mean ± SD Hg content in jaguars from the study site influenced by gold mining (SB) was compared to jaguars sampled in the area free of gold mining activities (CA) using a one-way ANOVA. The mean Hg content in jaguars from SB (673.0 ± 916.8 µg g-1) is significantly different from jaguars sampled in CA (29.7 ± 23.3 µg g-1), p = 0.03. The maximum recorded content of Hg was 2,010.4 ± 150.5 µg g-1, highest level ever recorded in a wild animal. The data indicate that Hg is an important threat to jaguars within at-risk regions of the Pantanal.

https://doi.org/10.1590/0001-3765201720170190 1753 downloads

Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism
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Schiavoni, Vagner Sarraipo

; Silva, Jairo Pinheiro da

; Lizarte Neto, Fermino Sanches

; Assis, Múcio Luiz Cirino de

; Tazima, Maria de Fátima Galli Sorita

; Carvalho, Camila Albuquerque Melo de

; Tirapelli, Daniela Pretti da Cunha

; Carlotti Jr, Carlos Gilberto

; Colli, Benedicto Oscar

; Tirapelli, Luis Fernando

Acta Cirúrgica Brasileira

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aug 2018, Volume 33 N. 8 Pages 652 - 663

Abstract: En | Text: En | PDF: En

Abstract Purpose: To evaluate histopathological and ultrastructural changes and expression of proteins related to apoptosis CASPASE 3 and XIAP after experimental induction of temporary focal cerebral ischemia (90 minutes) due to obstruction of the middle cerebral artery in alcoholism model. Methods: Forty adult Wistar rats were used, subdivided into 5 experimental groups: control group (C); Sham group (S); Ischemic group (I); Alcoholic group (A); and Ischemic and Alcoholized group (I+A): animals submitted to the same treatment of group A and after four weeks were submitted to focal cerebral ischemia during 90 minutes, followed by reperfusion of 48 hours. Were processed for histopathological analysis and immunohistochemistry (for the protein expression of CASPASE -3 and XIAP). Results: Greater histopathological changes were observed in the animals of groups I and I+A in the three areas analyzed. The neuronal loss was higher in the medial striatum region of the animals of groups I and I + A. The protein expression of CASPASE -3 was higher than that of XIAP in the groups I and I + A for both proteins. Conclusion: The expression of XIAP was slightly higher where the histopathological changes and expression of CASPASE -3 was less evident.

https://doi.org/10.1590/s0102-865020180080000001 877 downloads

Variação na dieta de Artibeus lituratus e Sturnira lilium (Chiroptera: Phyllostomidae) em três regiões fitogeográficas no sul do Brasil
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Bôlla, Daniela A. S

; Carvalho, Fernando

; Gazarini, Janaina

; Zocche, Jairo J

; Pedro, Wagner A

Mastozoología neotropical

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jun 2018, Volume 25 N. 1 Pages 05 - 16

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Phyllostomidae possui a maior diversidade morfológica e ecológica dentre os morcegos neotropicais desempenhando importantes papéis em ambientes florestais, tal como a dispersão de sementes. O objetivo do presente estudo foi comparar a dieta de Artibeus lituratus e Sturnira lilium em três regiões fitogeográficas do bioma Mata Atlântica - Floresta Ombrófila Densa (FOD), Floresta Estacional Semidecidual (FES) e Formação Pioneira com Influência Marinha (FPIM) - na Região Sul do Brasil. Os morcegos foram amostrados mensalmente com redes de neblina, seguindo o protocolo padrão para análise de dieta. Analisamos os dados de composição da dieta conforme atributos de riqueza, abundância e similaridade entre as três regiões amostradas (escalonamento multidimensional não métrico - NMDS e Índice de Bray-Curtis). Para A. lituratus e S. lilium o maior número de amostras provém da FES. Em todas as três regiões fitogeográficas, Ficus e Cecropia foram os gêneros mais consumidos por A. lituratus e Solanum por S. lilium. A abundância dos itens alimentares de A. lituratus e S. lilium foi influenciada pelas regiões fitogeográficas com a presença de agrupamentos distintos entre as amostras de FES, FOD e FPIM. A diferenciação da composição das dietas de A. lituratus e S. lilium entre as três fitofisionomias analisadas corrobora a hipótese de que a estrutura da vegetação influencia a composição da dieta dos morcegos. Essa diferença pode ser detectada apenas quando os itens alimentares são identificados em nível de espécie ou morfo-espécie.

The family Phyllostomidae has the greatest morphological and ecological diversity among Neotropical bats, playing important roles in forest environments. This study aimed to compare the diet of Artibeus lituratus and Sturnira lilium in three phytophysiognomies in the Atlantic Forest biome in Southern Brazil - Ombrophilous Dense Forest (FOD), Semideciduous Seasonal Forest (FES) and Restinga (FPIM). Bats were sampled monthly along a year using mist nets. Bat's diet was analyzed in feces following a standardized protocol. We analyzed data on diet composition by richness, abundance and similarity among the three sampled regions (Non-metric Multidimensional Scaling and Bray-Curtis Index). Samples from FES were the most abundant for both A. lituratus and S. lilium, with Ficus and Cecropia being the most consumed genera for the first species and Solanum for the second one in all sampled sites. The abundance of feeding items varied across phytogeographic regions.

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Ampliação de distribuição de Eumops patagonicus (Chiroptera: Molossidae) e primeiro registro em ambiente de restinga na costa leste do Brasil
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Carvalho, Fernando

; Bôlla, Daniela A. S

; Patel, Filipe M

; Miranda, João M. D

; Althoff, Sérgio L

; Zocche, Jairo J

Mastozoología neotropical

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dec 2017, Volume 24 N. 2 Pages 443 - 450

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Eumops patagonicus possui como limite leste de distribuição a província de Missiones na Argentina. Essa nota reporta a ampliação do limite leste de distribuição desta espécie e descreve o primeiro registro em Santa Catarina. Os indivíduos foram capturados no litoral norte do Estado, em ecossistema de Restinga. O registro amplia em 610 km o limite leste de distribuição e corresponde ao primeiro registro confirmado em Santa Catarina e na costa brasileira.

Eumops patagonicus has its eastern range limit in Misiones, Argentina. We report the extension of the easternmost range limit of E. patagonicus and describe the first record for this species from Santa Catarina, Southern Brazil. The individuals were captured in the northern coast of the state, in a Restinga ecosystem. Records described herein extend the species’ range 610 km east and are the first from the state of Santa Catarina and along the Brazilian coast.

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Evidence of the Presence of Thyroid Hormones in Achatina fulica Snails
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LUSTRINO, DANILO

; SILVA, ALBA C.M.

; ARAUJO, IRACEMA G.

; TUNHOLI, VICTOR M.

; TUNHOLI-ALVES, VINÍCIUS M.

; CASTRO, ROSANE N.

; CARVALHO, DENISE P.

; PINHEIRO, JAIRO

; MARASSI, MICHELLE P.

Anais da Academia Brasileira de Ciências

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2017, Volume 89 N. 3 Pages 2181 - 2188

Abstract: En | Text: En | PDF: En

ABSTRACT The objective of this study was to identify thyroid hormones and to examine their putative site of synthesis in Achatina fulica snails. For this purpose, radioimmunoassays were performed for T3 and T4 before and after long starvation with or without hemolymph deproteinization. Sodium/iodide symporter activity in vivo was analyzed through 125I administration with and without KClO4 pretreatment. Only T4 was detected, and its concentration decreased due to starvation or deproteinization. However, high-performance liquid chromatography analysis also showed the presence of T2 and T3 apart from T4, but rT3 was not detected in the A. fulica hemolymph. The sodium/iodide symporter activity was greater in cerebral ganglia than digestive gland, but KClO4 treatment did not inhibit iodide uptake in any of the tissues analyzed. Altogether, our data confirm for the first time the presence of thyroid hormones in A. fulica snails and suggest their participation in the metabolism control in this species, although the putative site of hormone biosynthesis remains to be elucidated.

https://doi.org/10.1590/0001-3765201720160698 1146 downloads

Nutritional status of jatropha under cattle manure and natural phosphate in rainfed conditions
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Lima, Rosiane L. S. de

; Azevedo, Carlos A. V. de

; Gheyi, Hans R.

; Carvalho Júnior, Genelicio S.

; Cazetta, Jairo O.

; Arriel, Nair H. C.

Revista Brasileira de Engenharia Agrícola e Ambiental

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nov 2015, Volume 19 N. 11 Pages 1028 - 1034

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMOHá pouca informação para embasar a recomendação técnica de adubação para a cultura do pinhão-manso na região Semiárida. Objetivou-se, com este trabalho, avaliar o estado nutricional de plantas de pinhão-manso fertilizadas com esterco bovino e fosfato natural em condições de sequeiro. O ensaio foi conduzido em delineamento de blocos ao acaso, com 3 repetições e distribuído em esquema fatorial 4 x 4, composto por 4 doses de esterco bovino (0, 4, 8 e 12 t ha-1) e 4 doses de fosfato natural (0, 250, 500 e 750 kg ha-1). A aplicação de 8 t ha-1 de esterco bovino, isolada ou associada a 500 kg ha-1 de fosfato natural, propicia condições adequadas para a obtenção de plantas de pinhão-manso com níveis considerados suficientes (g kg-1) de N (29,8), P (5,1), K (33,8), Ca (17,1), Mg (14,0) e S (2,6) e micronutrientes (Cu = 29,8 e Mn = 94,4 mg kg-1) no tecido foliar desta oleaginosa.

ABSTRACTThere is little information on the technical recommendation of fertilization for jatropha in the semi-arid region. This study aimed to evaluate the nutritional status of jatropha plants fertilized with cattle manure and natural phosphate under rainfed conditions. The experiment was set in a randomized block design, with three replicates, arranged in a 4 x 4 factorial scheme, corresponding to 4 doses of cattle manure (0, 4, 8 and 12 t ha-1) and 4 doses of natural phosphate (0, 250, 500 and 750 kg ha-1). The application of 8 t ha-1 of cattle manure, in isolation or associated with 500 kg ha-1 of natural phosphate, promotes adequate conditions to obtain jatropha plants with sufficient levels (g kg-1) of N (29.8), P (5.1), K (33.8), Ca (17.1), Mg (14.0) and S (2.6) and micronutrients (Cu = 8.0 and Mn = 94.4 mg kg-1) in its leaf tissue.

https://doi.org/10.1590/1807-1929/agriambi.v19n11p1028-1034 857 downloads

10.

Nutrient allocation among stem, leaf and inflorescence of jatropha plants
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Lima, Rosiane L. S. de

; Gheyi, Hans R.

; Azevedo, Carlos A. V. de

; Sofiatti, Valdinei

; Carvalho, Genelicio S.

; Cazetta, Jairo O.

Revista Brasileira de Engenharia Agrícola e Ambiental

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aug 2015, Volume 19 N. 8 Pages 760 - 766

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

<title>RESUMO</title><p>Ainda não se dispõe, para a cultura do pinhão-manso, de informações concernentes à compartimentalização dos nutrientes nos diversos órgãos da planta; essas informações são importantes para subsidiar a recomendação de adubação. Objetivou-se, com este trabalho, avaliar os teores de macro e micronutrientes no caule, nas folhas e inflorescências dos ramos de pinhão-manso por ocasião do início do florescimento. Plantas adultas de pinhão-manso foram amostradas durante o início do florescimento dividindo-se a planta em cinco compartimentos (inflorescências, folhas colhidas de ramos vegetativos, folhas colhidas de ramos floríferos, caules de ramos floríferos e caules de ramos vegetativos). As inflorescências do pinhão-manso são um forte dreno de nutrientes. As folhas são importantes fontes de nutrientes demandados pelas inflorescências por ocasião do início da floração. A maior compartimentalização de nutrientes nas inflorescências sugere a necessidade de adubações preventivas/corretivas que devem ser realizadas pelo menos 30 dias antes do florescimento disponibilizando, para as plantas, nutrientes em quantidades adequadas para a obtenção de boa produtividade.</p>

<title>ABSTRACT</title><p>Information on the partitioning of nutrients among various organs in jatropha plants, as a complementary tool for the recommendation of fertilization, is still not available. This study aimed to evaluate the contents of macro and micronutrients in stems, leaves and inflorescences of jatropha branches at the beginning of flowering. At the beginning of flowering, adult jatropha plants were sampled and divided into five compartments: inflorescences, leaves from vegetative branches, leaves from flowering branches, stems from vegetative branches and stems from flowering branches. Jatropha inflorescences are a drain of nutrients. Leaves are important sources of nutrients demanded by the inflorescences at the beginning of flowering. The higher allocation of nutrients in the inflorescences suggests the need for preventive/corrective fertilizations, which must be performed at least 30 days before flowering, providing plants with nutrients in adequate amounts for a good yield.</p>

https://doi.org/10.1590/1807-1929/agriambi.v19n8p760-766 1875 downloads

11.

IV Diretriz Brasileira sobre Dislipidemias e Prevenção da Aterosclerose: Departamento de Aterosclerose da Sociedade Brasileira de Cardiologia
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Sposito, Andrei C.

; Caramelli, Bruno

; Fonseca, Francisco A. H.

; Bertolami, Marcelo C.

; Afiune Neto, Abrahão

; Souza, Aguinaldo David

; Lottenberg, Ana Maria Pitta

; Chacra, Ana Paula

; Faludi, André A.

; Loures-Vale, Andréia A.

; Carvalho, Antônio Carlos

; Duncan, Bruce

; Gelonese, Bruno

; Polanczyk, Carisi

; Rodrigues Sobrinho, Carlos Roberto M.

; Scherr, Carlos

; Karla, Cynthia

; Armaganijan, Dikran

; Moriguchi, Emílio

; Saraiva, Francisco

; Pichetti, Geraldo

; Xavier, Hermes Toros

; Chaves, Hilton

; Borges, Jairo Lins

; Diament, Jayme

; Guimarães, Jorge Ilha

; Nicolau, José Carlos

; Santos, José Ernesto dos

; Lima, José Jayme Galvão de

; Vieira, José Luiz

; Novazzi, José Paulo

; Faria Neto, José Rocha

; Torres, Kerginaldo P.

; Pinto, Leonor de Almeida

; Bricarello, Liliana

; Bodanese, Luiz Carlos

; Introcaso, Luiz

; Malachias, Marcus Vinícius Bolívar

; Izar, Maria Cristina

; Magalhães, Maria Eliane C.

; Schmidt, Maria Inês

; Scartezini, Mariléia

; Nobre, Moacir

; Foppa, Murilo

; Forti, Neusa A.

; Berwanger, Otávio

; Gebara, Otávio C. E.

; Coelho, Otávio Rizzi

; Maranhão, Raul C.

; Santos Fº, Raul Dias dos

; Costa, Rosana Perim

; Barreto, Sandhi

; Kaiser, Sérgio

; Ihara, Silvia

; Carvalho, Tales de

; Martinez, Tania Leme Rocha

; Relvas, Waldir Gabriel Miranda

; Salgado, Wilson

Arquivos Brasileiros de Cardiologia

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apr 2007, Volume 88 Pages 2 - 19

Abstract: Pt | Text: Pt | PDF: Pt

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12.

Diketopiperazines produced by an Aspergillus fumigatus Brazilian strain
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Furtado, Niege A. J. C.

; Pupo, Mônica T.

; Carvalho, Ivone

; Campo, Vanessa L.

; Duarte, Marta Cristina T.

; Bastos, Jairo K.

Journal of the Brazilian Chemical Society

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nov 2005, Volume 16 N. 6b Pages 1448 - 1453

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Sete dicetopiperazinas, correspondentes aos ciclos (L)-Pro-(L)-Phe, (L)-Pro-Gly, (L)-Pro-(L)-Pro, (L)-Pro-(L)-Val, (L)-4-OH-Pro-(L)-Leu, (L)-4-OH-Pro-(L)-Phe e (L)-Pro-(L)-Leu, foram isoladas do caldo da cultura do fungo Aspergillus fumigatus. As estereoquímicas relativas e absolutas foram determinadas, respectivamente, com base nos experimentos de NOESY e através de modificação do método de Marfey em CLAE.

Seven diketopiperazines, corresponding to the cyclos (L)-Pro-(L)-Phe, (L)-Pro-Gly, (L)-Pro-(L)-Pro, (L)-Pro-(L)-Val, (L)-4-OH-Pro-(L)-Leu, (L)-4-OH-Pro-(L)-Phe, and (L)-Pro-(L)-Leu, were isolated from the Aspergillus fumigatus fermentation broth. The relative and absolute stereochemistries were determined on the basis of NOESY experiments and by using a modified version of Marfey's method using HPLC, respectively.

5180 downloads

13.

Qualidade fisiológica de sementes de sorgo coletadas em diferentes pontos de um secador
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Silva, Jadir N. da

; Cardoso Sobrinho, José

; Carvalho, Jairo A. de

; Dias, Denise C. F. dos S.

; Reis, Fernando P.

Revista Brasileira de Engenharia Agrícola e Ambiental

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dec 2001, Volume 5 N. 3 Pages 487 - 491

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Neste trabalho, avaliou-se a qualidade fisiológica e se quantificaram os possíveis gradientes de umidade de sementes de sorgo BR 601, durante o armazenamento. Utilizaram-se amostras coletadas em secador de camada fixa vertical, com duto central perfurado e distribuição radial de ar. Os pontos amostrais no secador se distanciavam 0,10; 0,30 e 0,50 m em relação à lateral do duto central de ar e nas alturas de 0,85; 2,02 e 3,64 m da base do secador, com capacidade de 8 t, além de uma amostra média (M) que representou os três pontos radiais, respectivamente. Armazenou-se o sorgo durante nove meses e se avaliou a germinação, vigor e umidade do produto no terceiro, sexto e nono meses de armazenamento. Não houve diferença na qualidade fisiológica das sementes nas diferentes alturas e distâncias em relação ao duto central de ar do secador, em relação à média (M) durante o armazenamento, mas no decorrer deste, a queda de qualidade foi mais evidente no teste de frio, obtida no nono mês de armazenamento.

The objective of this work was to evaluate the physiological quality and possible moisture gradients in seeds of sorghum BR 601, during storage. Samples from a vertical fixed-bed dryer with a perforated central duct and radial air distribution were used. In the dryer, the sample points were located at 0.10, 0.30 and 0.50 m distances in relation to the lateral of the central air duct. A composite sample was also collected and used as the control in relation to the others, at the respective heights. These sampling procedures were followed at heights of 0.85; 2.02 and 3.64 m from the dryer base. Within each height and distance relative to the air duct, the collected samples were stored during nine months and evaluated for germination and vigor. At the third, sixth and ninth months after storage, the samples were evaluated for possible differences in quality and seed moisture gradient in comparison to the composite sample. No differences were observed in the physiological quality of the seeds from either different heights or distances relative to the hot-air central duct, in comparison to the composite sample, during the storage time. In general, seed vigor diminished with time while the decreasing quality was most evident in a cold test, conducted after nine months of storage.

1555 downloads

14.

Woody Gall symptoms in different citrus species and varieties artificially colonized by the citrus brown aphid Toxoptera citricidus
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CARVALHO, SERGIO A.

; MÜLLER, GERD W.

; POMPEU JR, JORGINO

; CASTRO, JAIRO LOPES DE

Fitopatologia Brasileira

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mar 2001, Volume 26 N. 1 Pages 95 - 98

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Plântulas de 41 espécies e variedades de citros (Citrus spp.) foram maciçamente colonizadas com pulgões pretos (Toxoptera citricidus), obtidos de laranjeiras (Citrus sinensis) 'Pêra' com acentuados sintomas de tristeza induzida pelo complexo "Capão Bonito" do vírus da tristeza dos citros (Cistrus tristeza virus, CTV) com o objetivo de avaliar inicialmente a resposta em relação àquele complexo de CTV. A ocorrência de caneluras nas plantas foi negativa, mesmo após oito meses da inoculação. No entanto, foram observadas galhas lenhosas, sintomas típicos do vírus da enação das nervuras-galha lenhosa, nos porta-enxertos 'Volkameriano Palermo' (Citrus volkameriana) (73% das plantas com sintomas), 'Volkameriano Catania 2', (60%), Rangpur Lime D22.30 (20%), Volkameriano Australian Red, híbrido de limão Cravo 2, tângelo 'Orlando' (Citrus reticulata x Citrus paradisi) e limão (Citrus janmbhiri) 'Rugoso da Florida' (13%) e citrange (Poncirus trifoliata x Citrus sinensis) 'Carrizo' (7%). O maior número e as maiores galhas foram observadas em 'Volkameriano Palermo', mostrando ser esta a melhor indicadora para testes biológicos. Não existem relatos anteriores sobre a ocorrência de galhas em tangelo 'Orlando' e citrange 'Carrizo'.

Seedlings of 41 different citrus species and varieties were massively colonized with the citrus brown aphid Toxoptera citricidus, obtained from Pêra sweet orange (Citrus sinensis) trees, presenting symptoms of the "Capão Bonito" complex of the Citrus tristeza virus (CTV). The objective was to evaluate resistance or tolerance of the varieties to that virus complex, but even after eight months of inoculation no stem pitting was observed in the plants. Otherwise, the presence of galls similar to those induced by the vein enation-woody gall disease was observed in 73% of the plants of Volkamer Palermo (Citrus volkameriana), 60% of the Volkamer Catania 2, 2% of the Rangpur Lime D.22.30 (Citrus limonia), 13% of the Volkamer Australian Red, the Rangpur Lime hybrid, the Orlando tangelo (Citrus reticulata x Citrus paradisi) and the Florida Rough lemon (C. jambhiri), and 7% of the Carrizo citrange (Poncirus trifoliata x Citrus sinensis). The highest incidence and the largest gall size were observed in the Palermo Volkamer showing that this clone would be the most suitable to be used as an indicator plant in biological indexing tests for the disease. There are no previous reports in the literature about the occurrence of woody galls in Orlando tangelo and Carrizo citrange.

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