Resumo A hibernação é uma condição natural dos animais que vivem na zona temperada, embora alguns lagartos tropicais também experenciem hibernação anualmente, como é o caso do lagarto nativo da América do Sul, Salvator merianae ou “teiú”. Embora as características fisiológicas e metabólicas associadas à hibernação tenham sido amplamente estudadas, possíveis alterações na integridade das hemácias durante esse período ainda permanecem obscuras. A desidratação e o jejum são consequências naturais da hibernação por vários meses e podem estar relacionadas a algumas modificações celulares. Neste estudo, investigamos se a tolerância osmótica de hemácias do lagarto teiú sob hibernação são diferentes das células obtidas de animais em atividade normal. Além disso, investigamos indiretamente por meio da quantificação de biomoléculas oxidadas e da atividade de enzimas antioxidantes se a membrana das hemácias dos teiús em hibernação poderia estar associada à oxidação. Nossos resultados sugerem que as hemácias possuem maior fragilidade durante o período de hibernação, embora não tenhamos encontrado evidências de um cenário de estresse oxidativo associado à essa fragilidade acentuada. Embora não tenhamos excluído a possibilidade de dano oxidativo durante a hibernação, sugerimos que um aumento no volume das hemácias como consequência de sangue hipoosmótico durante a hibernação também poderia afetar a integridade de hemácias, tal como foi observado.

Abstract Hibernation is a natural condition of animals that lives in the temperate zone, although some tropical lizards also experience hibernation annually, such as the lizard native from South America, Salvator merianae, or “tegu” lizard. Even though physiological and metabolic characteristic associated with hibernation have been extensively studied, possible alterations in the red blood cells (RBC) integrity during this period remains unclear. Dehydration and fasting are natural consequences of hibernating for several months and it could be related to some cellular modifications. In this study, we investigated if the osmotic tolerance of RBCs of tegu lizard under hibernation is different from the cells obtained from animals while normal activity. Additionally, we indirectly investigated if the RBCs membrane of hibernating tegus could be associated with oxidation by quantifying oxidized biomolecules and the activity of antioxidant enzymes. Our findings suggest that RBCs are more fragile during the hibernation period, although we did not find evidence of an oxidative stress scenario associated with the accentuated fragility. Even though we did not exclude the possibility of oxidative damage during hibernation, we suggested that an increased RBCs volume as a consequence of hypoosmotic blood during hibernation could also affect RBCs integrity as noted.

Pheromones are essential for colony organization in honeybees. The pheromones 9-ODA ((E)-9-oxodec-2-enoic acid) and 9-HDA ((E)-9-hydroxydec-2-enoic acid), produced by the mandibular gland of the queen, have various functions within the colony, including inhibition of ovarian development of workers, attraction of swarms, and stabilization of the cluster group. An example of reaction of the colony to adverse conditions is absconding, which is defined as mass exit of all of the adult individuals, leaving even brood and food. In this study, the pheromones 9-ODA and 9-HDA were determined in virgin and normal mated, egg-laying European Carniolan and Africanized queens, as well as in mated queens that were in absconding colonies, using liquid chromatography with UV detection developed methodology. Absconding was induced by maintaining free-flying five-standard-Langstroth-frame colonies in a chamber artificially heated to 45 ºC. The obtained results showed that the amount of 9-ODA in Africanized queens (6.56 µg bee-1) is very low compared with European queens. However, large amounts of 9-HDA were found in the queens of absconding Africanized swarms (107.4 µg bee-1). According to our results, the quantities of 9-ODA and 9-HDA in Africanized honeybees in Brazil may be contributing to the high rates of absconding, promoting low effect of stabilizing agent, and high effect of attraction of dispersers cluster.

The aim of this study was to preliminary evaluate the lipid profile alterations on Pitcairnia flammea leaves based on variations in solvent proportion and ultrasonic ice bath extraction time, followed by a lipid class-enriched analysis employing chemometric techniques. Ultra-high performance liquid chromatography coupled to electrospray ionization mass spectrometry (UHPLC-ESI-MS) was used to acquire raw data and MS-DIAL and MetaboAnalyst platforms were used to perform data preprocessing and statistical analysis. The statistical analysis of UHPLC-ESI-MS data in both ionization modes enabled the visualization of a trend distribution based on extraction time. Furthermore, we were able to establish that the solvent proportion had a greater impact on group separation in data samples extracted for 30 min versus 10 and 20 min. Moreover, diacylglycerol or/and lysophosphatidylcholine are lipid subclasses that can be favored depending on the extraction time in the mass spectrometry analyses using positive electrospray ionization mode.

Toads of the Rhinella genus have a pair of paratoid glands that store biological secretions of high toxicity and varied chemical composition, rich in biologically active compounds. The present work aimed to carry out the investigation of the metabolomic profile and evaluation of the biological potential of the secretion paratoid glands (PGS) from Rhinella granulosa. The paratoid secretion was collected in the Piauí state (Brazil), extracted with methanol and the extract was analyzed by ultra-performance liquid chromatography with quadrupole time-of-flight mass spectrometry. Fifty chemical constituents were identified. The extract showed cytotoxicity against tumor cell lines of the central nervous system (half maximal inhibitory concentration (IC50) = 1.9 µg mL-1) and prostate (IC50 = 1.6 µg mL-1), unsatisfactory antimicrobial potential (minimal inhibitory concentration (MIC) > 312 µg mL-1) and inhibited the enzyme acetylcholinesterase (IC50 = 5.119 mg mL-1). The results presented relevant information about the PGS and contributed to the understanding of the metabolomic and biological potential of R. granulosa.

Resumen Este artículo analiza los factores biológicos de los elasmobranquios más comunes en Bahía Santa Elena, en el Área de Conservación Guanacaste en el Pacifico norte de Costa Rica. Debido a un vacío de información científica marino-costera que origina un fallo del sistema de manejo, no hay enfoque de resiliencia en las poblaciones sujeto de conservación. Se utilizó el método de muestreo mensual desde septiembre de 2015 hasta agosto de 2016. Mediante censo subacuático, uso de un trasmallo y visita en los puestos de recibo de pescado de Cuajiniquil se determinó la diversidad, abundancia relativa, distribución de las especies, aspectos biométricos, biología reproductiva y estructura de tallas de captura; también se aplicó una encuesta a 120 pescadores para conocer la percepción social en factores del desarrollo sostenible. Se trabajó con una muestra de 289 individuos que dio como resultado que los meses de mayor precipitación se relacionan con picos de los índices de diversidad, con poca distribución espacial, donde se presenta un remplazo estacional de especies, entre las que destaca el arribo de Rhinopterasteindachneri, Mobulamunkiana, durante los meses lluviosos y Mustelushenlei junto con Sphyrnalewini durante los vientos alisios. El periodo de desove principal para Myliobatiformes se ubica antes de diciembre, pero Urotrygonnana presenta un segundo periodo en julio. Se concluye que la biometría indica un mayor número de organismos juveniles para tiburones y adultos para rayas. La pesca con trasmallo de fondo se caracterizó por bajo rendimiento económico, lo que incide en la captura de juveniles de S. lewini, y este ingreso económico no se beneficia de los bienes naturales intangibles presentes en BSE que son patrimonio del Estado.

Abstract This article analyzes the biological factors of the most common elasmobranchs in Bahía Santa Elena, in the Guanacaste Conservation Area in the north Pacific of Costa Rica. Due to a lack of marine-coastal scientific information there is a system failure of management, leaving without a resilience approach to the populations subject to conservation. The monthly sampling method was used from September 2015 to August 2016, diversity, relative abundance, species distribution, biometric aspects, reproductive biology and catch size structure were determined by means of an underwater census, the use of a trammel net and a visit to the Cuajiniquil fish reception posts. A survey was applied to 120 fishermen to know the social perception regarding the factors of sustainable development. I worked with a sample of 289 individuals, among the main results, it was obtained that the months with the highest rainfall are related to peaks of the diversity indices, with little spatial distribution. A seasonal replacement of species is presented, highlightingthe arrival of Rhinopterasteindachneri, Mobulamunkiana, during the rainy months and Mustelushenlei and Sphyrnalewini during the Trade Winds. The main spawning period for Myliobatiformes is located before December, however Urotrygonnana presents a second period in July. It is concluded that biometry indicates a greater number of juvenile organisms for sharks and adults for rays, bottom gillnet fishing was characterized by low economic performance, which affects the capture of juveniles of S. lewini, this economic income does not benefit from the intangible natural assets present in BSE that are State assets.

The assessment of chlorine, fluorine, and sulfur concentrations in depth profile of drill cuttings was performed using ion chromatography after sample preparation by pyrohydrolysis. The parameters for pyrohydrolysis were optimized using a Box-Behnken design, and the established conditions were 300 mg of sample, 300 mg of V2O5, temperature of 1000 °C, reaction time of 25 min, and absorbing solution of 50 mmol L-1 NH3 + 150 mmol L-1 H2O2. The accuracy was evaluated by the analysis of certified reference materials and recovery tests, and the results showed a good agreement between determined and certified values and recoveries (89-108%). The precision (relative standard deviation (RSD) < 10%), and the limits of detection (0.5-2.5 mg kg 1) were suitable for drill cuttings analysis. The concentration of chlorine, fluorine, and sulfur in drill cuttings at different depths was at mg g-1 range, and its variation is related to the mineral composition of the samples.

RESUMO O rabanete e a alface são duas hortaliças que podem ser consorciadas, porque são culturas companheiras que se complementam. Assim, este estudo teve como objetivo avaliar retornos agro-bioeconômicos advindos do consórcio rabanete-alface em diversas quantidades equitativas de biomassa de jitirana (Merremia aegyptia) e flor-de-seda (Calotropis procera) em diferentes densidades populacionais de alface, em ambiente semiárido. O delineamento experimental utilizado foi em blocos ao acaso, com os tratamentos dispostos em esquema fatorial 4 × 4, com quatro repetições. O primeiro fator consistiu em quantidades equitativas de biomassa de M. aegyptia e C. procera nas doses de 20, 35, 50 e 65 Mg ha-1 em base seca. O segundo fator compreendeu quatro densidades populacionais de alface de 150, 200, 250 e 300 mil plantas ha-1. As cultivares de alface e rabanete plantadas foram ‘Tainá’ e ‘Crimson Gigante’, respectivamente. Retornos agro-bioeconômicos expressivos deste consórcio de rabanete-alface foram obtidos na razão equivalente de terra (RET) de 2,25, escore da variável canônica Z de 3,00 e na renda liquida (RL) de 52.270,48 R$ ha-1, respectivamente, na quantidade de biomassa de 65 Mg ha-1 dos adubos verdes e densidade populacional de alface de 300 mil plantas ha-1. Além disso, registrou-se também um índice de eficiência produtiva (IEP) de 0,96 e uma razão competitiva (RC) de 2,61 na quantidade de biomassa dos adubos de 65 Mg ha-1 e densidade de alface de 150 mil plantas ha-1. A utilização de biomassa de M. aegyptia e C. procera do bioma Caatinga, mostrou-se uma tecnologia viável para produtores que praticam o cultivo de rabanete e alface em consórcio em ambiente semiárido.

ABSTRACT Radish and lettuce are two crop vegetables that can be intercropped because they are companion cultures that complement each other. Thus, this study aimed to evaluate agro-bioeconomic returns arising from radish-lettuce intercropping in different equitable amounts of hairy woodrose (Merremia aegyptia) and roostertree (Calotropis procera) biomass at different population densities of lettuce, in the semi-arid environment. The experimental design was in randomized blocks, with treatments arranged in a 4 × 4 factorial scheme with four replicates. The first factor consisted of equitable amounts of M. aegyptia and C. procera biomass at doses of 20, 35, 50, and 65 Mg ha-1 on a dry basis. The second factor comprised four lettuce population densities of 150, 200, 250, and 300 thousand plants ha-1. The lettuce and radish cultivars planted were ‘Tainá’ and ‘Crimson Gigante’, respectively. Expressive agro-bioeconomic returns from this radish-lettuce intercropping were obtained in the land equivalent ratio (LER) of 2.25, a score of the canonical variable Z of 3.00, net income (NI) of 52,270.48 R$ ha-1, respectively, in the biomass amount of 65 Mg ha-1 of the green manures and lettuce population density of 300 thousand plants ha-1. Also, there was a productive efficiency index (PEI) of 0.96 and a competitive ratio (CR) of 2.61 in the biomass amount of green manure of 65 Mg ha-1 and lettuce density of 150 thousand plants ha-1. Using M. aegyptia and C. procera biomass from the Caatinga biome proved to be a viable technology for producers who practice the cultivation of radish and lettuce in intercropping in the semi-arid environment.

Considering the relevance of natural products to the development of new drugs, this study focuses on the species Sida rhombifolia (L.), Malvaceae, widely used as a traditional remedy in several countries. The species is used to treat several diseases such as fevers, skin diseases, stomach pain, diarrhea, gum infection, conjunctivitis, urinary infections and inflammation. Many researchers around the word have investigated its pharmacological potential, and many uses have been confirmed. In spite of that, the phytochemical composition of its extracts is still poorly investigated. This research aims to make advances on the understanding of the constituents of S. rhombifolia identifying its alkaloidal compounds using ultra-high performance liquid chromatography coupled to mass spectrometry (UPLC-QTOF-MS/MS). By analyzing its alkaloidal fraction, it was possible to identify: 11-cryptolepine carboxylic acid (1), cryptolepine (2), quindoline (3), 11 methoxyquidoline (4), quindolinone (5), cryptolepinone (6), and 11-quindoline methyl ester (7). The alkaloids 1 and 7 are being reported for the first time from Sida genus. Based on the antimicrobial potential of cryptolepinone-type alkaloids, we prepared by semi-synthesis two cryptolepinone derivatives: 10-methylcryptolepinone (8) and the unreported compound 10-ethylcryptolepinone (9). Both derivatives were tested against fungal and bacterial strains and 10-methylcryptolepinone (8) showed strong activity against Cryptococcus neoformans, Candida albicans, Candida tropicalis, Aspergillus fumigatus and Aspergillus flavus.

Abstract Two different reactive concentrated magnesium hydroxide (HM) pulps, produced in a lab reactor mill, and a synthetic and non-reactive HM-pulp, for comparative analyses, have been prepared and studied. Sodium polyacrylate was selected as a dispersant for improving the HM pulp fluidity, with concentrations varying from 0.42% to 2.0% on a dry basis. The two reactive pulps differed from each other mainly by the impurity level and for both, a residual MgO amount was detected, implying that the hydration reaction could proceed. Results (with and without dispersant) confirmed the formation of particle clusters as HM concentration rose until a critical value, above which the pulp behaved as a solid. Reactive HM-pulps with high impurities may represent a limiting factor in the drying operation. Among all HM-pulps studied, the purest reactive pulp with 1% dispersant presented the best flow behavior making this reactive pulp the most suitable to be tested in a pilot spray dryer.

Resumo Na pesca, o fenômeno fishing down food webs, ou ‘pescando teias tróficas abaixo’, expressa a redução do nível trófico na composição das capturas, e tem origem tradicionalmente atribuída à sobrepesca. Sob intenso extrativismo, a resiliência das espécies carnívoras, predadoras de topo, é particularmente afetada, sendo seus estoques os primeiros a entrarem em depleção. A cavala, ou serra, ou sororoca, Scomberomorus brasiliensis, é exemplo de recurso predador historicamente alvo da pesca artesanal, de pequena escala, na costa brasileira. O presente trabalho analisa os valores de desembarque do recurso em três períodos ao longo de 50 anos na costa paranaense, sul do Brasil, com objetivo de avaliar se sua produção tem efetivamente diminuído. Ainda, estuda-se a dieta da espécie na região, para confirmar seus hábitos carnívoros e avaliar o nível trófico na região. Os resultados mostram que, surpreendentemente, dos anos 1970 para 2019 a produção de S. brasiliensis tem aumentado em valores absolutos, bem como relativamente à de outros recursos, em que pese o nível trófico 4,238, similar a outras espécies do gênero. Constata-se que a pesca da cavala na região de estudo constitui um caso de aumento nos desembarques a despeito de elevado nível trófico e grande tamanho corpóreo. Os resultados concordam com recente levantamento em nível mundial, que nega existir correlação necessária entre sobrepesca e nível trófico alto, mas fatores que podem explicar os resultados do presente trabalho são discutidos.

Abstract In fisheries, the phenomenon known as fishing down food webs is supposed to be a consequence of overfishing, which would be reflected in a reduction in the trophic level of landings. In such scenarios, the resilience of carnivorous, top predator species is particularly affected, making these resources the first to be depleted. The Serra Spanish mackerel, Scomberomorus brasiliensis, exemplifies a predator resource historically targeted by artisanal fisheries on the Brazilian coast. The present work analyzes landings in three periods within a 50-year timescale on the Parana coast, Southern Brazil, aiming to evaluate whether historical production has supposedly declined. Simultaneously, the diet was analyzed to confirm carnivorous habits and evaluate the trophic level in this region. Surprisingly, the results show that from the 1970’s to 2019 Serra Spanish mackerel production grew relatively to other resources, as well as in individual values. The trophic level was calculated as 4.238, similar to other Scomberomorus species, consisting of a case where landings increase over time, despite the high trophic level and large body size of the resource. The results agree with a recent global assessment that has demystified a necessary correlation between high trophic level and overexploitation, but possible factors acting on the present findings are discussed.

Abstract Bacteria responsible for causing infections are common in hospital environments, water, soil, and food products. The infection risk is intensified by the absence of public sanitation, poor quality of life, and food scarcity. These external factors promote the dissemination of pathogens by direct contamination or biofilm formation. In this work, we identified bacterial isolates obtained from intensive care units in the southern region of Tocantins, Brazil. We compared matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) techniques and 16S ribosomal ribonucleic acid (rRNA) molecular analysis; we also performed phenotypic characterization. Fifty-six isolates characterized using morphotinctorial tests were classified as gram-positive (80.4%; n = 45) and gram-negative (19.6%; n = 11) and were resistant to several antibiotic classes; notably, we identified the blaOXA-23 resistance gene in the ILH10 isolate. Microbial identification using MALDI-TOF MS resulted in the identification of Sphingomonas paucimobilis and Bacillus circulans. 16S rRNA sequencing revealed four isolates belonging to the genera Bacillus and Acinetobacter. The similarity was superior to 99% for Acinetobacter schindleri in the Basic Local Alignment Search Tool (BLAST), grouped in the clade superior to 90%. Several strains isolated from intensive care units (ICU) were resistant to various antibiotic classes. These techniques allowed for the identification of several microorganisms of importance in public health, enabling improvements in human infection control and proving the quality of inputs, food, and water.

Resumo As bactérias responsáveis por causar infecções são comuns em ambientes hospitalares, água, solo e produtos alimentícios. O risco de infecção é intensificado pela ausência de saneamento público, má qualidade de vida e escassez de alimentos. Esses fatores externos promovem a disseminação de patógenos por contaminação direta ou formação de biofilme. Neste trabalho, identificamos isolados bacterianos obtidos de unidades de terapia intensiva na região sul do Tocantins, Brasil. Comparamos técnicas de espectrometria de massa de tempo de voo com ionização por dessorção a laser assistida por matriz (MALDI-TOF MS) e análise molecular de ácido ribonucleico ribossômico 16S (rRNA); também realizamos caracterização fenotípica. Cinquenta e seis isolados caracterizados por testes morfotintoriais foram classificados como gram-positivos (80,3%; n = 45) e gram-negativos (19,6%; n = 11) e foram resistentes a várias classes de antibióticos; notavelmente, identificamos o gene de resistência blaOXA-23 no isolado de ILH10. A identificação microbiana usando MALDI-TOF MS resultou na identificação de Sphingomonas paucimobilis e Bacillus circulans. O sequenciamento do 16S rRNA revelou quatro isolados pertencentes aos gêneros Bacillus e Acinetobacter. A similaridade foi superior a 99% para Acinetobacter schindleri no BLAST, agrupado no clado superior a 90%. Várias cepas isoladas de ICU foram resistentes a várias classes de antibióticos. Essas técnicas permitiram a identificação de diversos microrganismos de importância em saúde pública, possibilitando melhorias no controle de infecções humanas e comprovando a qualidade dos insumos, alimentos e água.

Abstract A model including simultaneous droplet heating and water evaporation is proposed to simulate temperature, shrinking and mass profiles of a spherical droplet subjected to convective drying, being valid for the first drying stage. Experimental data on drying skim milk and colloidal silica obtained in the literature were used for validation, but there is no restriction in the model that prevents it from being suitable for other materials. There were not significant differences observed concerning to the droplet components (dissolved or insoluble materials). The initial heating time of the particle upon reaching the constant temperature is relatively short ($\Delta t\approx7s$) for both simulated materials and water evaporation during the first drying stage occurs mostly at the wet bulb temperature of the air. Discrepancy between simulated and experimental values did not exceed $9\%$ for skim milk and $7\%$ for colloidal silica in this first stage, indicating good applicability of the model. Considering the applicability of the model in a more generic way, Whitaker correlation evaluated at the film temperature showed better results. Finally, the small discrepancy found is discussed and some improvements are proposed.

Abstract Considering the relevance of bell pepper and the limitations imposed by the deleterious effects of salt stress, especially in semi-arid regions, it is extremely important to establish strategies that can facilitate the use of saline water in vegetable production. In this scenario, this study aimed to evaluate the effect of the frequency of foliar application of salicylic acid on the morphophysiology and production of the bell pepper cv. “All Big” irrigated with saline water. The study was conducted at a greenhouse in Campina Grande - PB. The treatments were distributed in a completely randomized design and set up in a 4 × 4 factorial arrangement with three replications, corresponding to four application frequencies of salicylic acid (F1- No application of salicylic acid, F2 - Weekly application, F3- fortnightly application, and F4- monthly application) and four levels of electrical conductivity of irrigation water - ECw (0.8, 1.6, 2.4 and 3.2 dS m-1). The fortnightly application of salicylic acid at a concentration of 1.0 mM mitigated the effects of salt stress on the morphophysiology and production components of bell pepper cv. All Big cultivated with ECw of up to 2.4 dS m-1, which reinforces the hypothesis that salicylic acid can act as a signaling molecule and reduce the effects of saline stress in bell pepper, enabling the use of brackish water in agricultural activity, mainly in semi-arid regions of northeastern Brazil, which have a shortage of fresh water.

Resumo Considerando a relevância da cultura do pimentão e as limitações impostas pelos efeitos deletérios do estresse salino, principalmente em regiões semiáridas, é de suma importância o estabelecimento de estratégias que possam viabilizar o uso de águas salinas na produção de olerícolas. Neste contexto, objetivou-se com o presente estudo, avaliar o efeito da frequência de aplicação foliar de ácido salicílico sobre a morfofisiologia e a produção de pimentão cv. All Big irrigado com águas salinas. O estudo foi conduzido em casa de vegetação, em Campina Grande - PB. Os tratamentos foram distribuídos em delineamento inteiramente casualizados, em esquema fatorial de 4 × 4, com 3 repetições, sendo quatro frequências de aplicação de ácido salicílico (F1- Sem aplicação de ácido salicílico, F2 - Aplicação semanal, F3- quinzenal e F4- mensal) e quatro níveis de condutividade elétrica da água de irrigação - CEa (0,8, 1,6, 2,4 e 3,2 dS m-1). A aplicação quinzenal de ácido salicílico na concentração de 1,0 mM amenizou os efeitos do estresse salino sobre a morfofisiologia e os componentes de produção do pimentão cv. All Big cultivado com CEa de até 2,4 dS m-1, o que reforça a hipótese que o ácido salicílico pode atuar como uma molécula sinalizadora e reduzir os efeitos do estresse salino no pimentão, viabilizando o uso de água salobra na atividade agrícola, principalmente em regiões semiáridas do nordeste brasileiro, que possui escassez de água doce.

ABSTRACT This study examined the effects of cold working and subsequent annealing treatment for a short time on the corrosion behavior of a lean duplex stainless steel (LDSS). The as-received sample was solution treated at 1100 °C for 1800 s and cold rolled. The cold deformation (75%) was followed by isochronic annealing at 900, 950, 1000, 1050 and 1100 °C for 180 s. The microstructural changes were evaluated by scanning electron microscopy (SEM) and Vickers hardness, while corrosion behavior was investigated by cyclic potentiodynamic polarization technique and electrochemical impedance spectroscopy (EIS) in 3.5% NaCl solution at 22 ± 2 °C. After cyclic polarization, pits morphology was characterized by SEM. Cold rolling lowered lamellar spacing between the phases and increased hardness. Hardness at temperatures higher than 950 °C was equal to the 2304 LDSS solution treated. Although grain refinement is observed due to recrystallization, the corrosion resistance was not significantly improved. The highest pitting potential and charge transfer resistance were obtained after annealing at 1000 °C for 180 s. The pit distribution indicates that the corrosion behavior of the phases changed during the cold rolling and annealing treatment, and pitting was initiated preferentially in the α/γ boundaries and inside the α domains.