OBJECTIVE: Ischemic stroke may result from transient or permanent reductions of regional cerebral blood flow. Polymorphonuclear neutrophils have been described as the earliest inflammatory cells to arrive in ischemic tissue. CXCR1/2 receptors are involved in the recruitment of these cells. However, the contribution of these chemokine receptors during transient brain ischemia in mice remains poorly understood. In this work, we investigated the effects of reparixin, an allosteric antagonist of CXCR1/2 receptors, in a model of middle cerebral artery occlusion and reperfusion in mice. METHODS: C57BL/6J male mice treated with reparixin or vehicle were subjected to a middle cerebral artery occlusion procedure 1 h after the treatment. Ninety minutes after ischemia induction, the monofilament that prevented blood flow was removed. Twenty-four hours after the reperfusion procedure, behavioral changes, including motor signs, were analyzed with the SmithKline/Harwell/lmperial College/Royal Hospital/Phenotype Assessment (SHIRPA) battery. The animals were sacrificed, and brain tissue was removed for histological and biochemical analyses. Histological sections were stained with hematoxylin and eosin, neutrophil infiltration was estimated by myeloperoxidase activity and the inflammatory cytokine IL-iβ was measured by ELISA. RESULTS: Pre-treatment with reparixin reduced the motor deficits observed in this model of ischemia and reperfusion. Myeloperoxidase activity and IL-iβ were reduced in the reparixin-treated group. Histological analysis revealed that ischemic injury was also attenuated by reparixin pre-treatment. CONCLUSIONS: Our results suggest that the blockade of the CXCR1/2 receptors by reparixin promotes neuroprotective effects by reducing the levels of polymorphonuclear infiltration in the brain and the tissue damage associated with middle cerebral artery occlusion and reperfusion.

The manager nurse's challenge is to develop skills to implement actions which make a difference to the management model used by the nursing service. In this perspective, this paper reports on the experience of the Sensibilization for the Development of Management Skills Workshop, based on the model of laboratory education and involving manager nurses of a school hospital in the sate of Goiás, Brazil, in September, 2007. Management knowledge and skills make technical and human improvement possible, by better enabling the reading of the needs of the team in search for qualified assistance. In conclusion, the methodology adopted is efficient as a tool for continuing education.

El reto del enfermero gerente es desarrollar competencias para implementar acciones que hagan la diferencia en el modelo de gestión adoptado en el servicio de enfermería. En esa perspectiva, este artículo presenta informe de experiencia del Taller de Sensibilización para Desarrollo de Habilidades Gerenciales realizada en el modelo de educación de laboratorio, involucrando enfermeros gerentes de un hospital escuela del Estado de Goiás-Brasil, en septiembre de 2007. Los conocimientos y habilidades gerenciales posibilitaron perfeccionamiento técnico y humano, ampliando la capacidad de lectura de las necesidades del equipo en busca de asistencia calificada. Se concluye que la metodología adoptada es eficiente como herramienta para educación continuada.

O desafio do enfermeiro gerente é desenvolver competências para implementar ações que façam a diferença no modelo de gestão adotado no serviço de enfermagem. Nessa perspectiva, este artigo apresenta relato de experiência da Oficina de Sensibilização para Desenvolvimento de Habilidades Gerenciais realizada no modelo de educação de laboratório, envolvendo enfermeiros gerentes de um hospital escola do Estado de Goiás, em setembro de 2007. Os conhecimentos e habilidades gerenciais possibilitaram aperfeiçoamento técnico e humano, ampliando a capacidade de leitura das necessidades da equipe em busca de assistência qualificada. Conclui-se que a metodologia adotada é eficiente como ferramenta para educação continuada.