ABSTRACT Introduction: Chronic graft-versus-host disease (cGvHD) not only remains the main cause of late mortality after allogeneic hematopoietic cell transplant, but also has the capacity of causing severe organ impairment in those who survive. The Notch, a highly conserved ligand-receptor pathway, is involved in many immunological processes, including inflammatory and regulatory responses. Recently, mouse models have shown that the blockage of canonical Notch signaling prevents GvHD. Objective and Method: Due to the lack of data on the Notch pathway in human chronic GvHD, we sought to study the expression of NOTCH components in primary samples of patients who received allo-HCT and presented active cGvHD or a long-term clinical tolerance to cGvHD. Results: Our results showed a significantly lower expression of NOTCH components in both groups that received allo-HCT, independently of their cGvHD status, when compared to healthy controls. Conclusion: Moreover, there were no differences in gene expression levels between the active cGvHD and clinically tolerant groups. To our knowledge, this is one of the first studies performed in human primary samples and our data indicate that much remains to be learned regarding NOTCH signaling as a new regulator of GvHD. Introduction graftversushost graft versus host (cGvHD transplant survive ligandreceptor ligand receptor processes responses Recently GvHD Method alloHCT allo HCT longterm long term Results alloHCT, HCT, status controls Conclusion Moreover knowledge

OBJETIVO: A hemiparesia é um comprometimento parcial do hemicorpo que altera o equilíbrio, sendo este essencial para as atividades funcionais. OBJETIVO: Avaliar o equilíbrio em pacientes hemiparéticos submetidos ao treino de equilíbrio com o programa Wii Fit, que atuou como um recurso de biofeedback visual. MÉTODO: Foram selecionados 12 pacientes hemiparéticos pós AVE, 5 do sexo masculino e 7 do sexo feminino, com idade média de 58 ± 12,57 anos, divididos aleatoriamente em dois grupos. Um deles realizou a fisioterapia convencional (GC) pelo período de uma hora, o outro realizou por trinta minutos e mais trinta minutos de treino de equilíbrio com auxílio do Wii Fit (GW), duas vezes por semana durante cinco semanas, completando dez sessões. O equilíbrio foi avaliado antes e após as intervenções, por meio da aplicação da Escala de Equilíbrio de Berg (EEB) e pela estabilometria, que mensura a oscilação do centro de pressão (COP), nos eixos ântero-posterior (AP) e médio-lateral (ML), por uma plataforma de pressão em duas condições: de olhos abertos (OA) e olhos fechados (OF). RESULTADOS: De acordo com a EEB, os pacientes, tanto do GC quanto o do GW, obtiveram maior controle do equilíbrio estático e dinâmico. Na avaliação do COP no eixo ML, os indivíduos do GC e do GW tiveram diminuição na oscilação ML após a intervenção proposta para cada grupo, nas condições de OA e OF. No eixo AP do COP, o GC não teve diminuição na oscilação AP de OA e OF, e o GW apresentou diminuição na oscilação AP de OA e OF. CONLUSÃO: O presente estudo demonstra que a fisioterapia associada ao treino de equilíbrio com o Wii Fit apresenta resultados significantes na reabilitação dos indivíduos hemiparéticos, obtendo, assim, mais um recurso terapêutico na fisioterapia.

INTRODUCTION: TThe hemiparesis is a partial compromise of the hemisphere that alters the balance, which is essential for the functional activities. OBJECTIVE: To evaluate the balance in hemiparetic patients undergoing balance training with the Wii Fit (WG) program that served as a resource for visual biofeedback. METHOD: We studied 12 hemiparetic patients after stroke, 5 males and 7 females, mean age 58 ± 12.57 years old, were randomly divided into two groups. One group performed conventional physiotherapy (CP) for a period of one hour, the other group performed the traditional physical therapy for thirty minutes and thirty more minutes of balance training with the help of the Wii Fit (WG), twice weekly for five weeks, completing ten sessions. Balance was assessed before and after intervention by the application of the Berg Balance Scale (BBS) and stabilometry which measures the oscillation of pressure center (COP) in anteroposterior axis (AP) and mediolateral (ML), a pressure platform in two conditions, with eyes opened (EO) and eyes closed (EC). RESULTS: According to the BSE, patients from both the CG and the WG, have gained more control of static and dynamic balance. In assessing the COP in ML axis, the subjects of the CG and WG ML oscillation had decreased after the proposed intervention for each group, under the EO and EC. In the AP axis of the COP, the CG had no decrease in the AP oscillation of EO and EC, and WG had a decrease in the AP oscillation in OA and OF. CONCLUSION: This study shows that physiotherapy combined with balance training with Wii Fit presents significant results in the rehabilitation of hemiparetic subjects, thereby obtaining another therapeutic option in physiotherapy.