Abstract: Cerrado is the second largest biome in Brazil and majorly contributes to the country's grain production. Previous studies on soil metagenomics from the Cerrado revealed an outstanding microbial diversity. In this study, the abundance of pathogenic fungi was analyzed using metagenomic sequences of the Cerrado soils under native vegetation, and under agriculture with no-tillage and conventional tillage. In total, 128,627 sequences of fungi were identified, with 43,439 representing pathogenic fungi and were distributed as follows: native 17,301 (40%), no-tillage 13,780 (32%), and conventional tillage 12,358 (28%). We identified 41 pathogenic fungal species associated with human and animal infections. The data analysis revealed that the native soils had a higher relative abundance of fungal sequences, similar to pathogenic species sequences, in relation to the total eukaryotic sequences, than the conventional tillage and no-tillage treatments, which observed a reduction in fungal abundance because of anthropogenic activities.

Abstract To evaluate the release of bisphenol-A glycidyl methacrylate (BisGMA), triethylene glycol dimethacrylate (TEGDMA), bisphenol A (BPA), and phthalates of the composite resin used in the bonding of spurs applied in the treatment of children with anterior open bite and its effects on human keratinocytes. Methodology Saliva samples of 22 children were collected before spur attachment (baseline) and 30 minutes (min) and 24 hours (h) after spur bonding. Analysis was performed using high-performance liquid chromatography (HPLC) coupled to tandem mass spectrometry (HPLC–MS/MS) and gas chromatography coupled to mass spectrometry (GC–MS). Standardized resin increments were added to three different dilutions of the cell culture medium. Keratinocytes (HaCaT) were cultivated in the conditioned media and evaluated for cell viability (MTT) and cell scratch assay. Results The levels of BisGMA (1.74±0.27 μg/mL), TEGDMA (2.29±0.36 μg/mL), and BPA (3.264±0.88 μg/L) in the saliva after 30 min, in comparison to baseline (0±0 μg/mL, 0±0 μg/mL, and 1.15±0.21 μg/L, respectively), presented higher numbers. After 24 h, the levels of the monomers were similar to the baseline. Phthalates showed no significant difference among groups. HaCat cells showed increased viability and reduced cell migration over time after exposure to methacrylate-based resin composites. Conclusion Resin composites, used to attach spurs in children with anterior open bite during orthodontic treatment, release monomers after polymerization and can influence the behavior of human keratinocytes, even at very low concentrations. Orthodontists should be aware of the risks of the resinous compounds release and preventive procedures should be held to reduce patient exposure.

Abstract: Sugarcane cultivar RB961552 was developed by the Sugarcane Breeding Program of the Federal University of Alagoas, a member university of RIDESA. It was selected for being highly productive, responsive to fertigation and for the high leaf biomass and excellent inter-row closure. It is resistant to brown and orange rust and moderately resistant to leaf scald and smut diseases.

Abstract Sugarcane cultivar RB0442 has a high yield, late maturation and good drought tolerance. It is recommended for planting in restrictive environments, mainly with water-deficient soils, and is resistant to the major sugarcane diseases of the Northeastern region of Brazil.

Resumo O objetivo deste estudo foi avaliar o comportamento de leitões e porcas, frente ao tratamento de desgaste ou não de dentes do lactente. Foram utilizadas 67 matrizes suínas e 827 leitões. O experimento foi realizado em três locais e a obtenção de dados comportamentais foi a partir de etograma, na forma dirigida. As observações dos leitões foram realizadas de dez em dez minutos, durante quatro horas, três vezes por semana. Os dados foram analisados em delineamento inteiramente ao acaso com medidas repetidas no tempo. Utilizou-se o modelo misto com o efeito fixo de tratamento, turno, período e suas interações; e o efeito aleatório de lote utilizando o procedimento MIXED do SAS (2008). Foram testadas 15 estruturas de variância e covariância, escolhendo-se a que apresentou menor valor para o Critério de Informação de Akaike (AIC). Não foram encontrados efeitos significativos para o comportamento agonístico, estereotipado, lúdico e exploratório em relação aos tratamentos assim como para todo o período avaliado (P>0,05). Efeitos significativos foram identificados no comportamento de mamar, comparando-se os tratamentos (P≤0,05). Não foram encontrados efeitos significativos para o comportamento alimentar das matrizes (P>0,05). Comer diferiu de beber, para o período avaliado (P≤0,05). Com este estudo, conclui-se que o procedimento de desgaste ou permanência dos dentes de leitões pode influenciar em comportamentos importantes, como o de mamar, responsável pelo eficiente ganho de peso. O desgaste dos dentes pode ser desnecessário diante dos ferimentos causados, em decorrência do manejo errôneo desta pratica. Contudo, há necessidade de mais estudos direcionados a este tema, buscando maiores elucidações.

Abstract The objective of this study was to evaluate the behavior of piglets and sows regarding the treatment of wearing or not the teeth of the piglets. Sixty-seven swine matrices and 827 piglets were used. The experiment was performed in three locations and the behavioral data was obtained from the ethogram, in the directed form. Piglets were observed every ten minutes for four hours, three times a week. The data were analyzed in a completely randomized design with repeated measures in time. The mixed model was used with the fixed treatment effect, shift, period and their interactions, and the random batch effect using the MIXED procedure of SAS (2008). Fifteen structures of variance and covariance were tested, choosing the one that presented the lowest value for the Akaike Information Criterion (AIC). No significant effects were found for the agonistic, stereotyped, playful, and exploratory behavior in relation to the treatments as well as for the whole period evaluated (P>0.05). Significant effects were identified on the suckling behavior, comparing the treatments (P≤0.05). No significant effects were found for the dietary behavior of the matrices (P>0.05). The study differed from drinking for the period evaluated (P≤0.05). Permanence or not of the piglets' teeth can affect important behaviors such as suckling, which is responsible for the efficient weight gain. The wear of the teeth may be unnecessary due to the injuries caused as a result of the mismanagement of this practice. More studies on this theme, seeking further elucidations, are necessary

OBJETIVO: Determinar parâmetros anatômicos para localizar a inserção dos tendões flexores do joelho na tíbia. MÉTODOS: Foram selecionados 10 joelhos de cadáveres com estruturas mediais e anteriores íntegras. A dissecção foi feita por acesso ânteromedial até a exposição adequada da inserção dos tendões flexores (TF), do planalto tibial (PT) e da tuberosidade anterior da tíbia (TAT). Uma agulha 40×12 e um paquímetro digital foram usados para aferir a distância do planalto tibial da inserção dos tendões flexores do joelho a 15 mm da borda medial ao tendão patelar e da tuberosidade anterior da tíbia à inserção dos tendões flexores do joelho. O ângulo o planalto tibial e a inserção dos tendões flexores do joelho (PT-TF) foi calculado com o auxílio do software ImagePro Plus(r). RESULTADOS: A distância PT-TF foi de 41 ± 4,6 mm em média. A distância entre a TAT-TF foi de 6,88 ± 1 mm. A angulação (PT-TF) foi de 20,3 ± 4,9 graus. CONCLUSÃO: Na região anterior da tíbia os tendões flexores estão a cerca de 40 mm do planalto com um ângulo médio de 20 graus.

OBJECTIVE: To study the anatomy of the hamstring tendons insertion and anatomical rela-tionships. METHODS: Ten cadaver knees with medial and anterior intact structures were selected. The dissection was performed from anteromedial access to exposure of the insertion of the flexor tendons (FT), tibial plateau (TP) and tibial tuberosity (TT). A needle of 40 × 12 and a caliper were used to measure the distance of the tibial plateau of the knee flexor tendons insertion at 15 mm from the medial border of the patellar tendon and tibial tuberosity to the insertion of the flexor tendons of the knee. The angle between tibial plateau and the insertion of the flexor tendons of the knee (A-TP-FT) was calculated using Image Pro Plus software. RESULTS: The mean distance TP-FT was 41 ± 4.6 mm. The distance between the TT-FT was 6.88 ± 1 mm. The (A-TP-FT) was 20.3 ± 4.9°. CONCLUSION: In the anterior tibial flexor tendons are about 40 mm from the plateau with an average of 20°.

O objetivo do presente relato é descrever um caso de formação cicatricial exuberante, característica de queloide e configuração pseudo-tumoral em um jacaré-açu (Melanosuchus niger), macho, com idade estimada de 60 anos, pertencente ao Parque Zoobotânico do Museu Emílio Goeldi, situado em Belém, Pará, Brasil. A alteração manifestou-se no membro posterior direito, envolvendo as duas falanges distais do dedo lateral e mediu 12,4cm na maior largura. A neoformação foi removida cirurgicamente e amostras foram processadas e analisadas por histopatologia, que revelou crescimento constituído por tecido conjuntivo fibroso de morfologia habitual, estando estruturalmente maduro nas áreas mais centrais.

The objective of this note is to describe a case of exuberant scarring formation, with keloid characteristics and pseudo-tumoral configuration in a male Black caiman (Melanosuchus niger), with an estimated age of 60 years, belonging to the Zoobotanical Park at the Emílio Goeldi Museum, located in Belém, Pará, Brazil. The alteration appeared on the right posterior limb involving two distal phalanges of the lateral digit and measured 12.4cm at the greatest width. The keloid tissue was surgically removed and samples were processed and analyzed histopathologically, revealing growth made up of fibrous connective tissue with the habitual morphology, which was structurally mature in the more central areas.

Eimeria lagunculata, Eimeria mammiformis and Eimeria podocnemis n. spp., are described from the faeces of the fresh-water turtle Podocnemis expansa, in Pará State, north Brasil. Oocysts of E. lagunculata are ellipsoidal, 19.2 x 12.8 (17.0-20.7 x 11.8-14.1) mum, shape-index (= length/ width) 1.5 (1.4-1.7). Oocyst wall about 0.5-0.7 mum thick, with a prominent stopper-like micropyle at one pole. No oocyst residuum and no polar body. Sporocysts elongate ellipsoidal, 11.0 x 5.4 (10.4-11.8 x 5.2-6.0) mum, shape-index 2.0 (1.8-2.1): no Stieda body. A compact, ellipsoidal sporocyst residuum lies between the two sporozoites, which possess a posterior and an anterior refractile body. Oocysts of E. mammiformis broadly ellipsoidal, 30.0 x 19.4 (23.0-37.0 x 16.3-21.5) mum, shape-index 1.5 (1.1-1.9). Oocyst wall about 0.7 mum thick, with a prominent micropyle: no oocyst residuum and rarely a single polar body. Sporocysts ellipsoidal, 15.3 x 7.9 (14.8-17.0 x 7.4-9.6) mum, shape-index 2.0 (1.8-2.2), with a tiny Stieda body. Sporocyst residuum bulky, ellipsoidal: sporozoites with two conspicuous refractile bodies. E. podocnemis has broadly ellipsoidal oocysts, 17.0 x 12.8 (14.8-19.2 x 11.8-14.1) mum, shape-index 1.3 (1.1-1.4). Oocyst wall about 0.5-0.7 mum thick, with no micropyle. No oocyst residuum, but always a single polar body. Sporocysts ellipsoidal, 9.7 x 5.2 (8.9-10.4 x 4.4-6.0) mum, shape-index 1.9 (1.6-2.0), with no Stieda body. Sporocyst residuum bulky, ellipsoidal: sporocysts with 2 refractile bodies. Eimeria carinii n. sp., is recorded from the tortoise Geochelone denticulata, also from Pará. Oocyst wall about 1.2 mum thicl. No micropyle. Oocyst residuum limited to a number (about 10-20) of scattered granules: no polar body. Sporocysts broadly ellipsoidal, and with no Stieda body: they measure 8,8 x 7.3 (8.0-9.0 x 7.0-7.5) mum, shape-index 1.2 (1.1-1.3). Sporocyst residuum bulky, spherical to ellipsoidal: sporozoites possess both posterior and anterior refractile bodies.

Eimeria vitellini n. sp. is described from the faeces of the Rhamphastos v. vitellinus. Oocysts broadly ellipsoidal to oval (egg-shaped), 22,6 x 18.3 (20.0-25.0 x 16.3-22.5) micronm, shapeindex (length/width) 1.2 (1.1-1.1). Oocyst wall a single colourless layer about 0.5 micronm thick, becoming thinner at one ectremity, at which point the oocyst usually ruptures. No oocyst residuum, but 1 or 2 small polr bodies of about 1.0-1.5 x 0.5-1.0 micronm. Sporocysts ellongated ellipsoid (pearshaped), 14.3 x 7.5 (13.8-15.0 x 6.9-7.5) micronm, shape-index (1.9 (1.8-2.0), with a thin colourless wall bearing a very delicate Stieda body: a conspicuous sub-Stieda body is present. Sporozoites with anterior and posterior regractile bodies and strongly recurved around a bulky, compact sporocyst residuum composed of relatively fine granules and spherules.