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Vegetative propagation strategies in commercial pineapple cultivars
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Almeida, Maria do R. A. de

; Souza, Everton H. de

; Costa, Eva M. R.

; Souza, Fernanda V. D.

Revista Caatinga

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sep 2023, Volume 36 N. 3 Pages 513 - 523

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT The production of healthy pineapple seedlings for commercial plantations has been one of the main limiting factors of the species, and the search for improvements to meet this demand has been constant. Therefore, the present study aimed to compare different propagation strategies (natural tillering, stem sectioning, and micropropagation) in three commercial cultivars 'BRS Imperial', 'Pérola', and Gold 'MD-2' of pineapple. Three methods of vegetative propagation were evaluated: conventional, stem sectioning, and micropropagation. The evaluations consisted of the number of seedlings produced by each method and the period for their development until planting in the field. According to the conventional propagation technique results, the young seedling type in the cultivar 'Pérola' was more abundant. In the propagation by sectioning the stem, the 'BRS Imperial' cultivar presented the highest number of seedlings. Regarding the micropropagation technique, the highest production of shoots was observed in the third subculture for all cultivars, especially for ‘BRS Imperial’, which presented the highest total number of shoots in all subcultures. It was concluded that the micropropagation technique proved efficient in producing uniform seedlings on a large scale, in addition to having an advantage in the greater number of seedlings produced compared to the other methods studied.

RESUMO A produção de mudas sadias de abacaxizeiro para plantios comerciais tem sido um dos principais fatores limitantes para a cultura e a busca de melhorias para atender essa demanda tem sido constante. Diante disso, objetivou-se neste trabalho comparar diferentes estratégias de propagação em três cultivares comerciais de abacaxizeiro ‘BRS Imperial’, ‘Pérola’ e Gold ‘MD-2’. Foram analisados três métodos de propagação vegetativa: convencional, seccionamento do talo e micropropagação em relação as características número de mudas produzidas por cada método e o período para o seu desenvolvimento das mudas até o plantio em campo. De acordo com os resultados da técnica de propagação convencional, a muda tipo filhote, na cultivar ‘Pérola’, foi a que produziu em maior quantidade. Na propagação por seccionamento de talo, a cultivar ‘BRS Imperial’ foi a que apresentou o maior número de mudas. Em relação à técnica de micropropagação a maior produção de brotos foi observada no terceiro subcultivo em todas as cultivares, principalmente a ‘BRS Imperial’ que apresentou o maior número total de brotos. Concluiu-se que, a técnica de micropropagação de abacaxizeiro foi eficiente para a produção de mudas uniformes em larga escala, além de apresentar maior número de mudas produzidas em comparação aos outros métodos estudados.

https://doi.org/10.1590/1983-21252023v36n304rc

In vitro conservation of ‘Florida Rough’ lemon plants
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Carvalho, Mariane de Jesus da Silva de

; Souza, Antônio da Silva

; Santos, Emanuela Barbosa

; Soares Filho, Walter dos Santos

; Ledo, Carlos Alberto da Silva

; Costa, Eva Maria Rodrigues

; Souza, Fernanda Vidigal Duarte

Ciência Rural

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2022, Volume 52 N. 12 elocation e20210276

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O estabelecimento de condições de crescimento mínimo é fundamental para a conservação in vitro de germoplasma. Alterações nas concentrações do meio básico, na fonte de carbono, são fatores importantes na desaceleração do crescimento de plantas in vitro. Este trabalho teve como objetivo ajustar um protocolo de conservação in vitro de plantas do limoeiro ‘Rugoso da Flórida’. Microestacas de plantas previamente cultivadas in vitro, com aproximadamente 1 cm, foram inoculadas em tubos de ensaio contendo 10 mL do meio de cultura WPM em diferentes concentrações (1/1, 1/2 e 1/4), suplementado com 0; 12,5; 25 e 50 g.L-1 de sacarose, solidificado com 7 g.L-1 de ágar e o pH ajustado em 5,8. O experimento foi instalado em delineamento inteiramente casualizado com 15 repetições, em esquema fatorial 3 x 4, e mantido sob condições controladas durante 360 dias. Após este período, foram avaliadas altura de planta em cm (AP), número de folhas verdes (NFV), número de folhas senescentes (NFS), número de microestacas (NM) e massa da planta seca em g (MPS). As variáveis que mais contribuíram para explicar a diferença existente no comportamento das plantas do limoeiro ‘Rugoso da Flórida’ no meio de cultura WPM foram NFV e AP com 61,63 e 35,08%, respectivamente. O meio de cultura WPM na sua concentração original com a adição de 25 g.L-1 de sacarose promoveu os melhores resultados na redução do crescimento das plantas in vitro do limoeiro ‘Rugoso da Flórida’, mantendo-as fisiologicamente sadias.

ABSTRACT: The establishment of minimum growth conditions is essential for in vitro germplasm conservation. Changes to the basic medium and carbon source concentrations are important factors for reducing plant growth in vitro. This study adjusted a protocol for the in vitro conservation of ‘Florida Rough’ lemon plants. Microcuttings (approximately 1 cm) from plants that were previously cultivated in vitro were inoculated into test tubes with 10 mL of woody plant medium (WPM) at different concentrations (1/1, 1/2 and 1/4) and supplemented with 0, 12.5, 25 and 50 g.L-1 of sucrose, solidified with 7 g.L-1 agar and adjusted to pH 5.8. The experiment was completely randomized in a 3 x 4 factorial design with 15 replications and was maintained under controlled conditions for 360 days. After this period the plant height in cm (PH), the plant dry mass in g (PDM) and the 21 numbers of green leaves (NGL), senescent leaves (NSL) and microcuttings (NM) were evaluated. The variables that best explained the observed behavior of the ‘Florida Rough’ lemon plants were NGL and PH, with values of 61.63 and 35.08%, respectively. The original concentration of the WPM with the addition of 25 g. L-1 of sucrose yielded the best ‘Florida Rough’ lemon plant growth reduction in vitro while maintaining the physiologically health of the plants.

https://doi.org/10.1590/0103-8478cr20210276

THE PROFILE OF POTT’S DISEASE IN A SOUTH AMERICAN REFERENCE SERVICE
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Zanon, Igor de Barcellos

; Tsai, Vivian Lumi

; Ribeiro, Taiana Cunha

; Astur, Nelson

; Mendonça, Rodrigo Góes Medéa de

; Gotfryd, Alberto Ofenhejm

; Salles, Mauro José Costa

; Caffaro, Maria Fernanda Silber

; Klautau, Giselle Burlamaqui

; Meves, Robert

Coluna/Columna

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jun 2021, Volume 20 N. 2 Pages 109 - 113

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Objetivo: A tuberculose (TB) é uma das dez principais causas de morte no mundo. O início insidioso e os sintomas inespecíficos geralmente resultam em atraso do diagnóstico. O objetivo é avaliar as características dos pacientes com espondilodiscite tuberculosa em acompanhamento, em um hospital de referência sul-americano. Método: Avaliação retrospectiva de dados de prontuários, diagnóstico de tuberculose da coluna vertebral entre 2009 e 2018, de ambos os sexos, entre 0 e 80 anos de idade. As variáveis foram analisadas com base em grupos: epidemiológicas, clínicas, laboratoriais, microbiológicas, exames de imagem e tratamento. Resultados: Total de 26 casos, cerca de 80,8% do sexo masculino, média de idade de 41,6 ± 22,46 anos. A dor axial foi o sintoma mais prevalente (84,6%), o escore da EVA foi de 6,85 ± 2,87. A média de tempo entre o início dos sintomas e o diagnóstico foi de 23,8 ± 24,1 semanas (4-96). A região mais acometida foi a coluna torácica (50% dos casos). A maioria (61,4%) dos participantes apresentou função neurológica inalterada (Frankel D e E) no início do tratamento e 6 meses depois, sendo que 84,5% obtiveram melhora. No tratamento, 34,6% necessitaram de cirurgia, e a principal indicação foi déficit neurológico isolado (55,5%). O procedimento mais realizado foi descompressão e artrodese (55,5%). O tempo médio até a cura foi de 12,0 ± 8,8 meses (8-48). Conclusão: Doença de início insidioso, sintomas inespecíficos, grande frequência de exames microbiológicos negativos em casos com a doença. A menor parte dos casos necessitou de tratamento cirúrgico e a maioria apresentou boa recuperação neurológica. Nível de evidência IV; Série de casos.

ABSTRACT Objective: Tuberculosis (TB) represents one of the top ten causes of death in the world. Its insidious onset and nonspecific symptoms usually result in delayed diagnosis. The objective is to evaluate the characteristics of patients with tuberculous spondylodiscitis in follow-up at a South American reference hospital. Method: Retrospective evaluation of the medical records of patients of both sexes and between 0 and 80 years of age diagnosed with tuberculosis of the spine between 2009 and 2018. The variables were analyzed based on groups: epidemiological, clinical, laboratorial, microbiological, imaging tests and treatment. Results: Total of 26 cases, about 80.8% male, mean age 41.6 ± 22.46 years. Axial pain was the most prevalent symptom (84.6%), the VAS score was 6.85 ± 2.87. The mean time between symptom onset and diagnosis was 23.8 ± 24.1 weeks (4-96). The most affected region was the thoracic spine (50% of the cases). Most participants (61.4%) had no change in neurological function (Frankel D and E) at the beginning of treatment and after 6 months, and 84.5% improved. During treatment 34.6% required surgery and the main indication was isolated neurological deficit (55.5%). The most frequently performed procedure was decompression and arthrodesis (55.5%). The average time to cure was 12.0 ± 8.8 months (8-48). Conclusion: Disease with insidious onset, nonspecific symptoms, high frequency of negative microbiological tests in cases with the disease. A small number of the cases required surgical treatment and most of them achieved good neurological recovery. Level of evidence IV; Case series.

RESUMEN Objetivo: La tuberculosis (TB) es una de las diez principales causas de muerte en el mundo. El inicio insidioso y los síntomas inespecíficos generalmente resultan en atraso del diagnóstico. El objetivo es evaluar las características de los pacientes con espondilodiscitis tuberculosa en acompañamiento en un hospital de referencia sudamericano. Método: Evaluación retrospectiva de datos de historiales clínicos, diagnóstico de tuberculosis de la columna vertebral entre 2009 y 2018, de ambos sexos, entre 0 y 80 años de edad. Las variables fueron analizadas con base en grupos: epidemiológicas, clínicas, de laboratorio, microbiológicas, exámenes de imagen y tratamiento. Resultados: Total de 26 casos, cerca de 80,8% del sexo masculino, promedio de edad de 41,6 ± 22,46 años. El dolor axial fue el síntoma más prevalente (84,6%). El puntaje de la EVA fue de 6,85 ± 2,87. El promedio de tiempo entre el inicio de los síntomas y el diagnóstico fue de 23,8 ± 24,1 semanas (4-96). La región más acometida fue la columna torácica (50% de los casos). La mayoría (61,4%) de los participantes presentó función neurológica inalterada (Frankel D y E) al inicio del tratamiento y seis meses después, siendo que 84,5% obtuvo mejora. En el tratamiento, 34,6% precisó cirugía, y la principal indicación fue déficit neurológico aislado, (55,5%). El procedimiento más realizado fue descompresión y artrodesis (55,5%). El tiempo promedio hasta la cura fue de 12,0 ± 8,8 meses (8-48). Conclusión: Enfermedad de inicio insidioso, síntomas inespecíficos, alta frecuencia de exámenes microbiológicos negativos en casos con la enfermedad. La menor parte de los casos requirió tratamiento quirúrgico y la mayoría presentó buena recuperación neurológica. Nivel de evidencia IV; Serie de casos.

https://doi.org/10.1590/s1808-185120212002234844 267 downloads

Nutrición parenteral domiciliaria en España 2018. Informe del Grupo de Nutrición Artificial Domiciliaria y Ambulatoria NADYA
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Wanden-Berghe, Carmina

; Cuerda Compes, Cristina

; Maíz Jiménez, María Irene

; Pereira Cunill, José Luis

; Ramos Boluda, Esther

; Gómez Candela, Carmen

; Virgili Casas, Nuria

; Burgos Peláez, Rosa

; Luis Román, Daniel Antonio de

; Penacho Lázaro, Mª Ángeles

; Sánchez Martos, Eva Ángeles

; Martínez Faedo, Ceferino

; Díaz Guardiola, Patricia

; Álvarez Hernández, Julia

; Zugasti Murillo, Ana

; Campos Martín, Cristina

; Sanz Paris, Alejandro

; Martín Fontalba, María de los Ángeles

; Lobo, Gabriela

; Matía Martín, Pilar

; Carabaña Pérez, Fátima

; Martín Folgueras, Tomás

; Martín Palmero, Mª Ángela

; Luengo Pérez, Luis Miguel

; Sánchez-Vilar Burdiel, Olga

; Martínez Costa, Cecilia

; Tejera Pérez, Cristina

; Martínez Ramírez, María José

; García Delgado, Yaiza

; Ponce González, Miguel Á

; Mauri Roca, Silvia

; García Zafra, Mª Victoria

; Germán Díaz, Marta

; Romero Gabino, Victoria

; Molina Baeza, Begoña

; Gonzalo Marín, Montserrat

; Irles Rocamora, José Antonio

; Sánchez Sánchez, Rebeca

; Apezetxea Celaya, Antxón

; Joaquín Ortiz, Clara

; Suárez Llanos, José Pablo

; Pintor de la Maza, Begoña

; Leyes García, Pere

; Gil Martínez, Mª Carmen

; Carrera Santaliestra, María José

Nutrición Hospitalaria

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apr 2020, Volume 37 N. 2 Pages 403 - 407

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Aim: to communicate home parenteral nutrition (HPN) data obtained from the HPN registry of the NADYA-SENPE group (http://www.nadya-senpe.com) for the year 2018. Material and methods: descriptive analysis of the data collected from adult and pediatric patients with HPN in the NADYA-SENPE group registry from January 1st, 2018 to December 31st, 2018. Results: there were 278 patients from 45 Spanish hospitals (54.7 % women), 23 children and 255 adults, which represent a prevalence rate of 5.95 patients/million inhabitants/year 2018. The most frequent diagnosis in adults was “palliative cancer” (22.0 %), followed by “others”. In children it was Hirschsprung's disease together with necrotizing enterocolitis, with four cases (17.4 %). The first indication was short bowel syndrome in both children (60.9 %) and adults (35.7 %). The most frequently used type of catheter was tunneled in both children (81.0 %) and adults (41.1 %). Ending 75 episodes, the most frequent cause was death (52.0 %) and change to oral feeding (33.3 %). Conclusions: the number of centers and collaborating professionals in the registry of patients receiving HPN remains stable, as well as the main indications and reasons for termination of HPN.

Resumen Objetivo: comunicar los datos de nutrición parenteral domiciliaria (NPD) obtenidos del registro del grupo NADYA-SENPE (http://www.nadya-senpe.com) del año 2018. Material y métodos: análisis descriptivo de los datos recogidos de pacientes adultos y pediátricos con NPD en el registro NADYA-SENPE del 1 de enero al 31 de diciembre de 2018. Resultados: se registraron 278 pacientes (54,7 % mujeres), 23 niños y 255 adultos, procedentes de 45 hospitales españoles, lo que representa una tasa de prevalencia de 5,95 pacientes/millón de habitantes/año 2018. El diagnóstico más frecuente en adultos fue “oncológico paliativo” (22,0 %), seguido de “otros”. En niños fue la enfermedad de Hirschsprung junto con la enterocolitis necrotizante, con cuatro casos (17,4 %). El primer motivo de indicación fue síndrome de intestino corto tanto en niños (60,9 %) como en adultos (35,7 %). El tipo de catéter más utilizado fue el tunelizado tanto en niños (81,0 %) como en adultos (41,1 %). Finalizaron 75 episodios, la causa más frecuente fue el fallecimiento (52,0 %) y el paso a vía oral (33,3 %). Conclusiones: el número de centros y profesionales colaboradores en el registro de pacientes que reciben NPD se mantiene estable, así como las principales indicaciones y los motivos de finalización de la NPD.

https://doi.org/10.20960/nh.02976 397 downloads

Antimicrobial activity of propolis extract fractions against Staphylococcus spp. isolated from goat mastitis
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Santos, Heidy C. Dos

; Vieira, Dielson S.

; Yamamoto, Sandra M.

; Costa, Mateus M.

; Sá, Maria C.A.

; Silva, Eva M.S.

; Silva, Tania M.S.

Pesquisa Veterinária Brasileira

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dec 2019, Volume 39 N. 12 Pages 954 - 960

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O uso indiscriminado de antibióticos no tratamento de mastite caprina leva ao desenvolvimento de micro-organismos resistentes que poderão estar presentes em alimentos, colocando em risco a saúde humana. Dessa forma, a própolis surge como uma alternativa para o tratamento de doenças por possuir uma ação antimicrobiana, principalmente pela presença de flavonoides em sua composição. O objetivo desse estudo foi avaliar o potencial antimicrobiano da própolis frente à Staphylococcus spp. isolados de casos de mastite caprina e qualificar o extrato etanoico bruto por cromatografia líquida de alta eficiência (CLAE-DAD). Neste estudo, os valores de concentração bactericida mínima (CBM) dos extratos de própolis em álcool etílico, acetato de etila e hexano nos isolados foram de 6250, 3125 e 1562,5μg/mL, respectivamente. Pelo teste de aderência à microplacas, observou-se que 20,78% dos microorganismos, não foram capazes de formar biofilme, 14,70% foram classificados como moderados, 64,70% em fracos e nenhum como forte produtor de biofilme. A própolis em seus diferentes diluentes foi capaz de afetar a formação de biofilme e apresentou significativa atividade antimicrobiana frente a cepas de Staphylococcus spp., podendo ser indicada para utilização em estudos “in vivo”.

ABSTRACT: The indiscriminate use of antibiotics in the treatment of caprine mastitis causes the appearance of resistant microorganisms, besides leaving residues in milk, putting at risk to human health. In this way, propolis is an alternative in the treatment of diseases because it has antimicrobial activity, mainly because of the presence of flavonoids in its composition. The aim of this study was to evaluate the antimicrobial potential of propolis to Staphylococcus spp. Isolated from cases of goat mastitis and qualify the crude ethanoic extract by high performance liquid chromatography (HPLC). In this study, the minimum bactericidal concentration values of propolis extracts in ethanol, ethyl acetate and hexane showed that the best concentrations capable of promoting the highest mortality of the isolates of Staphylococcus spp. from mastitis in goats, were 6250, 3125 and 1562.5μg/mL, respectively. By the microplate adherence test, it was found that 20.78% isolates were not able to form biofilm, 14.70% were classified as moderate and 64.70% were weak and none as a strong biofilm producer. Propolis in its different diluents was able to affect the formation of biofilm and showed a pronounced marked antimicrobial activity against Staphylococcus spp. strains and may be indicated for use in in vivo studies.

https://doi.org/10.1590/1678-5150-pvb-5940 1156 downloads

Nutrición parenteral domiciliaria en España 2017. Informe del Grupo de Nutrición Artificial Domiciliaria y Ambulatoria NADYA
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Wanden-Berghe, Carmina

; Pereira Cunill, José Luis

; Cuerda Compes, Cristina

; Ramos Boluda, Esther

; Maiz Jiménez, María Irene

; Gómez Candela, Carmen

; Virgili Casas, Nuria

; Burgos Peláez, Rosa

; Pérez de la Cruz, Antonio

; Penacho Lázaro, Mª Ángeles

; Sánchez Martos, Eva Ángeles

; Luis Román, Daniel Antonio de

; Martínez Faedo, Ceferino

; Martín Fontalba, María de los Ángeles

; Álvarez Hernández, Julia

; Matía Martín, Pilar

; Díaz Guardiola, Patricia

; Carabaña Pérez, Fátima

; Sanz París, Alejandro

; Garde Orbaiz, Carmen

; Sánchez-Vilar Burdiel, Olga

; Martín Folgueras, Tomás

; Martín Palmero, Mª Ángela

; Luengo Pérez, Luis Miguel

; Zugasti Murillo, Ana

; Martínez Costa, Cecilia

; Suárez Llanos, José Pablo

; Tejera Pérez, Cristina

; Irles Rocamora, José Antonio

; Arraiza Irigoyen, Carmen

; García Delgado, Yaiza

; Campos Martín, Cristina

; Ponce González, Miguel Ángel

; Mauri Roca, Silvia

; García Zafra, Mª Victoria

; Morán López, Jesús M

; Molina Baeza, Begoña

; Gonzalo Marín, Montserrat

; Joaquín Ortiz, Clara

; Pintor de la Maza, Begoña

; Gil Martínez, Mª Carmen

; Carrera Santaliestra, María José

; Forga Visa, Mª del Talló

; Apezetxea Celaya, Antxón

; Sánchez Sánchez, Rebeca

; Urgeles Planella, Juan Ramón

Nutrición Hospitalaria

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dec 2018, Volume 35 N. 6 Pages 1491 - 1496

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Aim: to communicate HPN data obtained from the HPN registry of the NADYA-SENPE group (www.nadya-senpe.com) for the year 2017. Material and methods: descriptive analysis of the data collected from adult and pediatric patients with HPN in the NADYA-SENPE group registry from January 1st, 2017 to December 31st, 2017. Results: there were 308 patients from 45 Spanish hospitals (54.5% women), 38 children and 270 adults, with 3,012 episodes, which represent a prevalence rate of 6.61 patients/million inhabitants/year 2017. The most frequent diagnosis in adults was "palliative cancer" (25.6%), followed by "others". In children, it was Hirschsprung's disease with six cases (15.8%). The first indication was short bowel syndrome in both children (55.3%) and adults (33.7%). The most frequently used type of catheter was tunneled in both children (73.4%) and adults (38.2%). Ending 81 episodes, the most frequent cause was death (62.9%) and transition to oral feeding (34.7%). Conclusions: the progressive increase of collaborating centers and professionals in the registry of patients receiving NPD is maintained. The main indications of HPN and the motive for ending have remained stable.

Resumen Objetivo: comunicar los datos de nutrición parenteral domiciliaria (NPD) obtenidos del registro del grupo NADYA-SENPE (www.nadya-senpe.com) del año 2017. Material y métodos: análisis descriptivo de los datos recogidos de pacientes adultos y pediátricos con NPD en el registro NADYA-SENPE desde el 1 de enero al 31 de diciembre de 2017. Resultados: se registraron 308 pacientes (54,5% mujeres), 38 niños y 270 adultos, procedentes de 45 hospitales españoles, en total 312 episodios, lo que representa una tasa de prevalencia de 6,61 pacientes/millón de habitantes/año 2017. El diagnóstico más frecuente en adultos fue "oncológico paliativo" (25,6%), seguido de "otros". En niños fue la enfermedad de Hirschsprung, con seis casos (15,8%). El primer motivo de indicación fue síndrome de intestino corto tanto en niños (55,3%) como en adultos (33,7%). El tipo de catéter más utilizado fue el tunelizado tanto en niños (74,3%) como en adultos (38,2%). Finalizaron 81 episodios; la causa más frecuente fue el fallecimiento (62,9%) y que pasaron a vía oral (34,7%). Conclusiones: se mantiene el incremento progresivo de centros y profesionales colaboradores en el registro de pacientes que reciben NPD. Las principales indicaciones de NPD y de motivo de finalización se mantienen estables.

https://doi.org/10.20960/nh.2364 229 downloads

Lutzomyia umbratilis from an area south of the Negro River is refractory to in vitro interaction with Leishmania guyanensis
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Soares, Rodrigo Pedro

; Nogueira, Paula Monalisa

; Secundino, Nágila Francinete

; Marialva, Eric Fabrício

; Ríos-Velásquez, Cláudia Maria

; Pessoa, Felipe Arley Costa

Memórias do Instituto Oswaldo Cruz

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mar 2018, Volume 113 N. 3 Pages 202 - 205

Abstract: En | Text: En | PDF: En

BACKGROUND Lutzomyia umbratilis, the vector for Leishmania guyanensis in northern South America, has been found naturally infected with L. guyanensis only in areas north of the Negro and Amazon rivers. While populations of this sand fly species are also found in areas south of these rivers, these populations have never been reported to be infected and/or transmitting L. guyanensis. However, no studies on the corresponding host-parasite interactions are available. OBJECTIVES This study evaluated the interaction between Lu. guyanensis promastigotes and field-collected Lu. umbratilis sand flies from Rio Preto da Eva and Manacapuru, which are located to the north and south, respectively, of the Negro River. METHODS Procyclic and metacyclic attachment was quantified using an in vitro system. FINDINGS Low attachment of parasites to the midguts of insects collected from Manacapuru was detected. Conversely, greater binding of metacyclic parasites was observed in the midguts of insects collected from Rio Preto da Eva, and this attachment was more pronounced than that observed for procyclics (p < 0.03). MAIN CONCLUSIONS The Lu. umbratilis population from an area south of the Negro River has lower in vitro interaction with L. guyanensis. The higher attachment of L. guyanensis to midguts of insects from Rio Preto da Eva may suggest better vector competence. These findings are in accordance with previously reported epidemiological information of American cutaneous leishmaniasis (ACL) transmission in the Amazon.

https://doi.org/10.1590/0074-02760170425 1163 downloads

Análisis de aguas residuales con fines epidemiológicos: aplicaciones a la estimación del consumo de sustancias de abuso y en salud pública en general. Red española ESAR-Net
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Bijlsma, Lubertus

; Celma, Alberto

; González-Mariño, Iria

; Postigo, Cristina

; Andreu, Vicente

; Andrés-Costa, María Jesús

; Hernández, Félix

; Alda, Miren López de

; López-García, Ester

; Marcé, Rosa María

; Montes, Rosa

; Pocurull, Eva

; Picó, Yolanda

; Rodil, Rosario

; Rodríguez-Gil, José Luis

; Valcárcel, Yolanda

; Quintana, José Benito

Revista Española de Salud Pública

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2018, Volume 92 elocation e201808053

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT This manuscript introduces Wastewater-Based Epidemiology (WBE) and its potential in the assessment of diverse aspects related to public health. This methodology can provide data in a relatively short temporal and local scale (typically dialy-weekly at the municipal level) on consumption patterns of illicit drugs (e.g. cocaine or cannabis), licit substances of abuse (e.g. alcohol) by measuring their consumption biomarkers (i.e. the original unmetabolized substance or some of its metabolite) in wastewater. Besides discussing the fundaments, advantages and shortcomings of WBE, it reviews some of the main precedents at international level and most remarkable activities that have been taken place in this field in Spain. Finally, the Spanish Network of Wastewater-Based Epidemiology (ESAR-Net) as is presented. ESAR-Net is an Excellence Network that sums up the efforts of the most relevant Spanish researchers in the field of WBE, aiming to investigate future perspectives of this methodology and its impact on Public Health competences in Spain.

RESUMEN En este artículo se presenta la metodología de análisis de aguas residuales con fines epidemiológicos (wastewater-based epidemiology, WBE) y su potencial para abordar diversos aspectos relacionados con la salud pública. Esta metodología permite obtener datos a una escala temporal y espacial relativamente pequeña (típicamente datos diarios-semanales sobre un municipio) de hábitos de consumo de sustancias de abuso, ilegales (como la cocaína o el cannabis) o legales (como el alcohol) a través de la determinación de biomarcadores de consumo (el compuesto original no metabolizado o alguno de sus metabolitos) en el agua residual. Aparte de discutir los fundamentos, ventajas y limitaciones de WBE, se comentan los precedentes más relevantes a nivel internacional, y las actividades más destacables en España en este ámbito. Finalmente, se exponen, los objetivos de la Red Española de Análisis de Aguas Residuales con Fines Epidemiológicos (ESAR-Net), una “Red de Excelencia” que agrupa a investigadores españoles con amplia experiencia en el área de WBE, así como las perspectivas de futuro de esta metodología puede tener para mejorar las competencias de la Salud Pública en España.

Análisis de aguas residuales con fines epidemiológicos: aplicaciones a la estimación del consumo de sustancias de abuso y en salud pública en general. Red española ESAR-Net
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Bijlsma, Lubertus

; Celma, Alberto

; González-Mariño, Iria

; Postigo, Cristina

; Andreu, Vicente

; Andrés-Costa, María Jesús

; Hernández, Félix

; Alda, Miren López de

; López-García, Ester

; Marcé, Rosa María

; Montes, Rosa

; Pocurull, Eva

; Picó, Yolanda

; Rodil, Rosario

; Rodríguez-Gil, José Luis

; Valcárcel, Yolanda

; Quintana, José Benito

Revista Española de Salud Pública

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2018, Volume 92 elocation e201808053

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

1542 downloads

10.

Nutrición parenteral domiciliaria en España 2016: informe del Grupo de Nutrición Artificial Domiciliaria y Ambulatoria NADYA
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Wanden-Berghe, Carmina

; Virgili Casas, Nuria

; Ramos Boluda, Esther

; Cuerda Compes, Cristina

; Moreno Villares, José Manuel

; Pereira Cunill, José Luis

; Gómez Candela, Carmen

; Burgos Peláez, Rosa

; Penacho Lázaro, M.ª Ángeles

; Pérez de la Cruz, Antonio

; Álvarez Hernández, Julia

; Gonzalo Marín, Montserrat

; Matía Martín, Pilar

; Martínez Faedo, Ceferino

; Sánchez Martos, Eva Ángeles

; Sanz Paris, Alejandro

; Campos Martín, Cristina

; Martín Folgueras, Tomás

; Martín Palmero, M. Ángela

; Martín Fontalba, María de los Ángeles

; Luengo Pérez, Luis Miguel

; Zugasti Murillo, Ana

; Martínez Ramírez, María José

; Carabaña Pérez, Fátima

; Martínez Costa, Cecilia

; Díaz Guardiola, Patricia

; Tejera Pérez, Cristina

; Parés Marimón, Rosa M.ª

; Irles Rocamora, José Antonio

; Garde Orbaiz, Carmen

; Ponce González, Miguel Ángel

; García Zafra, María Victoria

; Sánchez Sánchez, Rebeca

; Urgeles Planella, Juan Ramón

; Apezetxea Celaya, Antxón

; Sánchez-Vilar Burdiel, Olga

; Joaquín Ortiz, Clara

; Suárez Llanos, José Pablo

; Pintor de la Maza, Begoña

; Leyes García, Pere

; Gil Martínez, M.ª Carmen

; Mauri Roca, Silvia

; Carrera Santaliestra, María José

Nutrición Hospitalaria

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dec 2017, Volume 34 N. 6 Pages 1497 - 1501

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Objective: To communicate HPN data obtained from the HPN registry of the NADYA-SENPE group (www.nadya-senpe.com) for the year 2016. Material and methods: Descriptive analysis of the data collected from adult and pediatric patients with HPN in the NADYA-SENPE group registry from January 1st, 2016 to December 31 st, 2016. Results: There were 286 patients from 42 Spanish hospitals (54.2% women), 34 children and 252 adults, with 294 episodes, which represent a prevalence rate of 6.16 patients / million inhabitants / year 2016. The most frequent diagnosis in adults was "palliative cancer" (25.8%), followed by "others". In children it was "motility alterations" with 6 cases (17.6%), Hirschsprung's disease and necrotising enterocolitis, both with 5 children (14.7%). The first indication was short bowel syndrome in both children (64.7%) and adults (37.3%), followed by intestinal obstruction in 28.6% adults and 14.7% in children. The most frequently used type of catheter was tunnelled in both children (70.6%) and adults (37.9%). The most frequent complication in adults was infection related to the catheter, which presented a rate of 0.48 infections / 1,000 days of NPD. During this period, 71 episodes ended in adults and the main cause was death (57.7%) followed by resuming the oral route (31%). Conclusions: There is a progressive increase of centers and professional collaborators in the registry who report patients receiving parenteral nutrition at home. The main indications of HPN and the motive for ending have remained stable.

Objetivo: comunicar los datos de nutrición parenteral domiciliaria (NPD) obtenidos del registro del Grupo de Nutrición Artificial Domiciliaria y Ambulatoria (NADYA-SENPE; www.nadya-senpe.com) del año 2016. Material y métodos: análisis descriptivo de los datos recogidos de pacientes adultos y pediátricos con NPD en el registro NADYA-SENPE desde el 1 de enero al 31 de diciembre de 2016. Resultados: se registraron 286 pacientes (54,2% mujeres), 34 niños y 252 adultos, procedentes de 42 hospitales españoles con 294 episodios, lo que representa una tasa de prevalencia de 6,16 pacientes/millón de habitantes/año 2016. El diagnóstico más frecuente en adultos fue de oncológico paliativo (25,8%), seguido de otros. En niños, fue de alteraciones de la motilidad con 6 casos (17,6%), la enfermedad de Hirschsprung y la enterocolitis necrotizante, ambos con 5 niños (14,7%). El primer motivo de indicación fue síndrome de intestino corto tanto en niños (64,7%) como en adultos (37,3%), seguido de obstrucción intestinal, 28,6% en adultos y 14,7% en niños. El tipo de catéter más utilizado fue el tunelizado tanto en niños (70,6%) como en adultos (37,9%), y la complicación más frecuente en adultos fue la infección relacionada con el catéter, que presentó una tasa de 0,48 infecciones/1.000 días de NPD. Durante este periodo, finalizaron 71 episodios en adultos siendo la causa de finalización principal el fallecimiento (57,7%) y paso a vía oral (31%). Conclusiones: se constata un incremento progresivo de centros y profesionales colaboradores en el registro de pacientes que reciben NPD. Las principales indicaciones de NPD y de motivo de finalización se mantienen estables.

https://doi.org/10.20960/nh.1686 538 downloads

11.

Effect of time and storage conditions on the physical and physico-chemical characteristics of the pulp of yellow and purple passion fruit
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ARAÚJO, Letícia da Silva

; COSTA, Eva Maria Rodrigues

; SOARES, Taliane Leila

; SANTOS, Idália Souza dos

; JESUS, Onildo Nunes de

Food Science and Technology

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jul 2017, Volume 37 N. 3 Pages 500 - 506

Abstract: En | Text: En | PDF: En

Abstract The aim of this study was evaluate the physical and physico-chemical characteristics of extracted fruit pulp of passion fruit with different skin color (yellow, light and dark purple) under refrigeration temperatures and storage times. The extracted pulp of passion fruit was stored at different temperatures: 10 °C, 25 °C; - 30 °C and - 80 °C and at four different storage periods: 0; 10; 20 and 30 days. The following physical and physico-chemical characteristics of the fruit were evaluated: color of fruit peel, fruit weight, diameter, length and width of the fruit, peel thickness, peel mass, pulp mass, pulp color, juice yield, pH, soluble solids, titratable acidity and ratio. The physical and physico-chemical results indicate that all the passion fruit pulp, in natura as well as refrigerated, presented values in conformity to the identity and quality standards. The coloration of the fruit peel influenced the preservation of pulp color during the storage period and conditions. Purple fruits showed no change in the color of the pulp, even under different conservation procedures. The passion fruit pulp can be stored up to 30 days while preserving the physical and physico-chemical characteristics, similar to the pulp in natura.

https://doi.org/10.1590/1678-457x.20616 4053 downloads

12.

Nutrición parenteral domiciliaria en España durante 2015: informe del Grupo de Nutrición Artificial Domiciliaria y Ambulatoria NADYA
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Wanden-Berghe, Carmina

; Campos Martín, Cristina

; Cuerda Compes, Cristina

; Gómez Candela, Carmen

; Burgos Peláez, Rosa

; Moreno Villares, José Manuel

; Pereira Cunill, José Luis

; Pérez de la Cruz, Antonio

; Virgili Casas, Nuria

; Martínez Faedo, Ceferino

; Álvarez Hernández, Julia

; Garde Orbaiz, Carmen

; Penacho Lázaro, M.ª Ángeles

; Sánchez Martos, Eva Ángeles

; Sanz Paris, Alejandro

; Gonzalo Marín, Montserrat

; Zugasti Murillo, Ana

; Matía Martín, Pilar

; Martín Folgueras, Tomás

; Carabaña Pérez, Fátima

; Díaz Guardiola, Patricia

; Tejera Pérez, Cristina

; Luis Román, Daniel de

; Luengo Pérez, Luis Miguel

; Santacruz Carmona, Nieves

; Apezetxea Celaya, Antxón

; Ponce González, Miguel Ángel

; Urgeles Planella, Juan Ramón

; Laborda González, Lucía

; Martínez Olmos, Miguel Ángel

; Sánchez-Vilar Burdiel, Olga

; Joaquín Ortiz, Clara

; Martínez Costa, Cecilia

; Suárez Llanos, José Pablo

; Calleja Fernández, Alicia

; Leyes García, Pere

; Gil Martínez, M.ª Carmen

; Mauri, Silvia

; García Zafra, María Victoria

; Carrera Santaliestra, Maria José

Nutrición Hospitalaria

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dec 2016, Volume 33 N. 6 Pages 1487 - 1490

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Aim: To communicate the results of the Spanish Home Parenteral Nutrition (HEN) registry of the NADYA-SENPE group for the year 2015. Material and methods: Data was recorded online by NADYA group collaborators that were responsible of the HPN follow-up from 1st January to 31st December 2015. Results: Two hundred and thirty-six patients with 243 episodes of NPD were recorded from 40 hospitals. This represents a rate of 5.08 patients/million habitants for 2015. The most frequent pathology in adults was other (26.3%) followed by palliative oncological (21.6%). The most common complication was catheter-related sepsis which presented a rate of 0.53 infections/1,000 days of HPN. Sixty-four episodes were finished; the main cause was death (43.7%) and resuming to oral via (32.8%). Conclusions: we find increasing centers and professional partners, responding to the progressively more patients with parenteral nutrition support at home. The main indications for the establishment of NPD and causes termination of treatment remain stable.

Objetivo: comunicar los datos del registro de Nutrición Parenteral Domiciliaria (NPD) del grupo de trabajo NADYA-SENPE del año 2015. Material y métodos: recopilación de los datos de NPD del registro online del grupo de Nutrición Artificial Domiciliaria y Ambulatoria (NADYA) desde el 1 de enero de 2015 al 31 de diciembre de 2015. Resultados: se registraron 236 pacientes, con 243 episodios de NPD procedentes de 40 hospitales, lo que representa una tasa de 5,08 pacientes/millón de habitantes/año 2015. La patología más frecuente en los adultos fue "otros" (26,3%) seguido por "oncológico paliativo" (21,6%). La complicación más frecuente fue la séptica relacionada con el catéter, que presentó una tasa de 0,53 infecciones/1.000 días de NPD. Finalizaron 64 episodios, la principal causa fue el fallecimiento (43,7%) y el "paso a la vía oral" (32,8%). Conclusiones: constatamos el aumento de los centros y profesionales colaboradores, dando respuesta a la cantidad progresivamente mayor de pacientes con soporte nutricional parenteral en domicilio. Se mantienen estables las principales indicaciones para el establecimiento de NPD y las causas de finalización del tratamiento.

https://doi.org/10.20960/nh.464 885 downloads

13.

Nutrición parenteral domiciliaria en España durante 2014: informe del Grupo de Nutrición Artificial Domiciliaria y Ambulatoria NADYA
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Wanden-Berghe, Carmina

; Pereira Cunill, José Luis

; Cuerda Compes, Cristina

; Moreno Villares, José Manuel

; Pérez de la Cruz, Antonio

; Burgos Peláez, Rosa

; Gómez Candela, Carmen

; Virgili Casas, Nuria

; Penacho Lázaro, M.ª Ángeles

; Martínez Faedo, Ceferino

; Garde Orbaiz, Carmen

; Gonzalo Marín, Montserrat

; Sanz Paris, Alejandro

; Álvarez, Julia

; Sánchez Martos, Eva Ángeles

; Martín Folgueras, Tomás

; Campos Martín, Cristina

; Matía Martín, Pilar

; Zugasti, Ana

; Carabaña Pérez, Fátima

; García Zafra, María Victoria

; Ponce González, Miguel Ángel

; Suárez Llanos, José Pablo

; Martínez Costa, Cecilia

; Luis, Daniel de

; Apezetxea Celaya, Antxón

; Luengo Pérez, Luis Miguel

; Díaz Guardiola, Patricia

; Gil Martínez, M.ª Carmen

; Olmo García, M.ª Dolores del

; Leyes García Grupo, Pere

; Vidal Casariego, Alfonso

; Joaquín Ortiz, Clara

; Sánchez-Vilar Burdiel, Olga

; Laborda González, Lucía

Nutrición Hospitalaria

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dec 2015, Volume 32 N. 6 Pages 2380 - 2384

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Aim: to communicate the results of the Spanish Home Parenteral Nutrition (HEN) registry of the NADYASENPE group for the year 2014. Material and methods: data was recorded online by NADYA group collaborators that were responsible of the HPN follow-up from 1st January to 31st December 2014. Results: a total of 220 patients and 229 episodes of HPN were registered from 37 hospitals that represents a rate of 4.7 patients/million habitants/year 2014. The most frequent disease in adults was other diseases (23.3%), neoplasm (20.4%) followed by radical active neoplasm (11.8%) and mesenteric ischemia (10.9%). The most frequent diagnosis for children were the congenital intestinal disorders (33.3%) followed by traumatic short bowel and other diagnosis. Conclusions: the number of participating centers and registered patients increased progressively respect to preceding years. We consider that the HPN should be regulated by the Sanitary Administration within the framework of the National Health Service Interregional Council. And its inclusion in the portfolio of health services of the different Autonomous Comunities would be beneficial for patients and professionals.

Objetivo: comunicar los datos del registro de Nutrición Parenteral Domiciliaria (NPD) del grupo de trabajo NADYA-SENPE del año 2014. Material y métodos: recopilación de los datos de NPD del registro "on-line" del grupo de Nutrición Artificial Domiciliaria y Ambulatoria (NADYA) desde el 1 de enero de 2014 al 31 de diciembre de 2014. Resultados: se registraron 220 pacientes, con 229 episodios de NPD, procedentes de 37 hospitales; lo que representa una tasa de 4,73 pacientes/millón de habitantes/ año 2014. Las patologías más frecuentes en los adultos fueron: ‘otros diagnósticos' (22,3%), la neoplasia paliativa (20,4%), seguida por la neoplasia activa radical (11,8%) y la isquemia mesentérica (10,9%). En los niños las patologías más frecuentes fueron: ‘alteraciones congénitas intestinales' (33,3%), seguidas por ‘intestino corto traumático' y ‘otro diagnóstico'. Conclusiones: se observa un aumento del número de pacientes que reciben nutrición parenteral domiciliaria y de los centros colaboradores. Consideramos que la NPD debería ser regulada por la Administración Sanitaria en el marco del Consejo Interterritorial del Sistema Nacional de Salud, y que su inclusión en la Cartera de Servicios de los diferentes Servicios de Salud de las diferentes comunidades autónomas redundaría en un beneficio para el paciente y los profesionales.

https://doi.org/10.3305/nh.2015.32.6.10056 677 downloads

14.

Permanent neonatal diabetes by a new mutation in KCNJ11: unsuccessful switch to sulfonylurea
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Lau, Eva

; Correia, Cintia

; Freitas, Paula

; Nogueira, Claúdia

; Costa, Maria

; Saavedra, Ana

; Costa, Carla

; Carvalho, Davide

; Fontoura, Manuel

Archives of Endocrinology and Metabolism

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dec 2015, Volume 59 N. 6 Pages 559 - 561

Abstract: En | Text: En | PDF: En

Permanent neonatal diabetes (PNDM) can result from activating heterozygous mutations in KCNJ11 gene, encoding the Kir6.2 subunit of the pancreatic ATP-sensitive potassium channels (KATP). Sulfonylureas promote KATP closure and stimulate insulin secretion, being an alternative therapy in PNDM, instead of insulin. Male, 20 years old, diagnosed with diabetes at 3 months of age. The genetic study identified a novel heterozygous mutation in exon 1 of the KCNJ11 gene – KCNJ11:c1001G>7 (p.Gly334Val) – and confirmed the diagnosis of PNDM. Therefore it was attempted to switch from insulin therapy to sulfonylurea. During glibenclamide institution C-peptide levels increased, however the suboptimal glycemic control lead us to restart an intensive insulin scheme. This new variant of KCNJ11 mutation had a phenotypic lack of response to sulfonylurea therapy. Age, prior poor metabolic control and functional change of KATP channel induced by this specific mutation may explain the observed unsuccessful switch to sulfonylurea. Interestingly, C-peptide levels raise during glibenclamide administration support some degree of improvement in insulin secretory capacity induced by the treatment. Understanding the response to sulfonylurea is crucial as successful treatment may be life-changing in these patients.

https://doi.org/10.1590/2359-3997000000076 1746 downloads

15.

O INSTRUMENTO DE COMPENSAÇÃO AMBIENTAL NO BRASIL E NO ESTADO DE MINAS GERAIS
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Barros, Eva Costa de

; Borges, Luis Antonio Coimbra

; Paula, Maria das Graças

; Mafra, Flávia Luciana Naves

CERNE

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sep 2015, Volume 21 N. 3 Pages 449 - 455

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A compensação ambiental para empreendimentos causadores de significativo impacto ambiental é um instrumento instituído pelo artigo 36 da lei n° 9.985/2000. O objetivo do trabalho foi descrever, analisar a legislação ambiental pertinente ao instrumento de Compensação Ambiental no Brasil e apresentar o estado de Minas Gerais como um estudo de caso. Para isto adotou-se as técnicas de pesquisa bibliográfica e pesquisa documental. O resultado demonstrou que a compensação ambiental aumentou no estado de Minas Gerais e pode ser uma ferramenta importante para auxiliar na conservação ambiental por meio das Unidades de Conservação.

ABSTRACT Compensation for environmental projects that cause significant environmental impact is an instrument established by Article 36 of Law No. 9.985/2000. The objective of this study was to describe and analyze relevant environmental legislation to the Environmental Compensation instrument in Brazil and use the state of Minas Gerais as a case study. For this we adopted the techniques of bibliographic and documentary research. The result showed that environmental compensation increased in the state of Minas Gerais and can be an important tool to help in environmental conservation through protected areas.

https://doi.org/10.1590/01047760201521031702 3631 downloads

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