Resumo Metais como cobre e prata podem ser extremamente tóxicos para bactérias em concentrações excepcionalmente baixas. Devido a esta atividade biocida, os metais têm sido amplamente utilizados como agentes antimicrobianos em uma infinidade de aplicações relacionadas à agricultura, saúde e indústria em geral. Muitos microrganismos vivem no ambiente humano e, se o equilíbrio dessas criaturas na natureza for alterado, a saúde dos indivíduos e da sociedade estará ameaçada devido à produção e emissão de odores desagradáveis e à redução dos padrões de saúde. A presença de microrganismos em têxteis pode causar efeitos adversos, como descoloração ou manchas, decomposição de materiais fibrosos, resistência reduzida e, eventualmente, deterioração. A maioria das fibras e polímeros não apresenta resistência aos efeitos dos micróbios e fornecem fatores de crescimento para os microrganismos, como temperatura e umidade adequadas, nutrientes do suor e gordura das glândulas da pele, células mortas da pele, bem como materiais usados na etapa de acabamento causando crescimento e disseminação de vários micróbios. Com o advento da nanotecnologia, diversas indústrias e o cotidiano humano passaram por mudanças. Nos últimos anos, o aumento da pesquisa em nanopartículas levou à produção de têxteis com maior eficiência e valor agregado. Esses têxteis modificados evitam a propagação de odores desagradáveis, a propagação e a transmissão de doenças. Este artigo analisa os fundamentos e princípios dos têxteis antimicrobianos, bem como uma breve visão geral dos materiais antimicrobianos e nanoestruturas com propriedades antimicrobianas. baixas biocida agricultura alterado adversos manchas fibrosos eventualmente deterioração adequadas pele nanotecnologia mudanças anos agregado doenças antimicrobianas

Abstract Metals, such as copper and silver, can be extremely toxic to bacteria at exceptionally low concentrations. Because of this biocidal activity, metals have been widely used as antimicrobial agents in a multitude of applications related with agriculture, healthcare, and the industry in general. A large number of microorganisms live in the human environment. if the balance of these creatures in nature is disturbed, the health of the individual and society will be threatened due to the production and emission of unpleasant odors and the reduction of health standards. The presence of microorganisms on textiles can cause adverse effects such as discoloration or staining on textiles, decomposition of fibrous materials, reduced strength, and eventually decay of textiles. Most fibers and polymers do not show resistance to the effects of microbes and by providing growth factors for microorganisms such as the right temperature and humidity, nutrients from sweat and fat from skin glands, dead skin cells as well as materials used in the stage of finishing the textiles causes the rapid growth and spread of various microbes. With the advent of nanotechnology, various industries and human daily life underwent changes. In recent years, increasing research on nanoparticles has led to the production of textiles with greater efficiency and added value. These modified textiles prevent the spread of unpleasant odors, the spread, and transmission of diseases. This article reviews the basics and principles of antimicrobial tetiles, as well as a brief overview of antimicrobial materials and nanostructures with antimicrobial properties. Metals silver concentrations activity agriculture healthcare general environment disturbed standards strength humidity glands nanotechnology changes years value diseases tetiles properties

Abstract Introduction Parapharyngeal space tumors with complex anatomy and diverse histology have remained a challenging phenomenon for treating physicians. Objectives We have conducted a comprehensive web search on the PubMed, Web of Science, EMBASE, Cochrane Library, Biomedical Literature Database (CBM), and Clinicaltrials.gov databases to determine the factors that are associated with postoperative complications in parapharyngeal space tumors. Data Synthesis Two researchers reviewed all identified articles independently with a third reviewer for adjudication. Patient demographics and other clinicopathological characteristics were explored. The systematic review has identified 631 benign parapharyngeal space tumors with neurogenic and salivary tissue histology in 13 studies, with a mean age of 42.9 ± 7.76 years old and a median follow-up of 40.98 ± 19.1 months. Salivary gland (50.8%) and neurogenic (49.1%) tumors were the most common histological entities. Tumor size, location, histology, deep parotid lobe involvement, and proximity to great vessels or to the skull base were the deciding factors in selecting the surgical approach. The factors considered to select the surgical approach do not seem to have a correlation with the outcome in terms of neurological sequalae (p = 0.106). Tumors with neurogenic histology have significantly increased chances of developing neurological complication (OR 6.07; p = 0.001). Conclusion Neurologic complications are significantly associated with neurogenic benign tumors rather than surgical approach.

Abstract Introduction Age and lymph node ratio have been attributed as independent predictors for survival and recurrence in carcinoma of unknown primary (CUP). Objective The purpose of this study was to analyze the prognostic value of p16 overexpression for CUP in the absence of true primary (TP). Methods The study involved 43 patients who underwent therapeutic lymph node dissection (LND) from 2000 to 2015 after all the diagnostic work up for CUP. Immunohistochemistry for p16 overexpression was performed. Cox proportional hazard regression analysis was used to analyze the prognostic impact on 5-year overall survival (OS) and recurrence-free survival (RFS). Results The male-to-female ratio was 5.1:1, with a median age of 62 years. The clinicopathological data, except for p16 overexpression, did not differ significantly in terms of 5-year OS and RFS. The Cox regression analysis proposed p16 positivity to be an independent prognosticator of regional recurrence-free survival (RRFS) (hazard ratio [HR] 6.180, p = 0.21). The median time to recurrence and death were 10 and 25 months, respectively. Conclusion Cervical metastasis with p16 overexpression is a significant prognostic factor of improved RFS after surgery in CUP. The prognostic significance of lymph node p16 positivity should be further studied.

Resumen Introducción: El aprendizaje asistido por pares (AAP) es un método de enseñanza que desarrolla nuevos conocimientos y habilidades a través del apoyo activo del aprendizaje por parte de los compañeros. Objetivos: Evaluar el impacto del AAP en la educación médica, mediante la recopilación de datos cuantitativos que exploran su eficacia. Evaluar la percepción y la satisfacción de los estudiantes con el AAP. Métodos: Se elaboró una encuesta con una escala de Likert de 5 puntos para los estudiantes que participaban en el programa AAP como tutores o como alumnos. Los datos se compararon y analizaron estadísticamente en función del género, el promedio académico y el año de estudio. Además, se realizó un seguimiento del rendimiento de los compañeros tutores y de los compañeros alumnos mediante la comparación de sus notas con las de los que no compartían el AAP y con su GPA del año anterior. Resultados: El análisis estadístico de los datos del cuestionario reveló que los estudiantes de medicina consideraban la enseñanza voluntaria entre pares de forma similar a la enseñanza en la facultad y tienen una percepción generalmente positiva de la AAP. La mayoría de los encuestados afirmaron haberse beneficiado tanto profesional como personalmente de las actividades de AAP. Recomendaron ampliar el papel de la AAP e incorporar oficialmente las sesiones de AAP en el plan de estudios. El rendimiento de los estudiantes aumentó significativamente en el caso de los que actuaron como alumnos y tutores de sus compañeros. Conclusiones: El uso de AAP junto con otros métodos de enseñanza y aprendizaje proporciona un valor adicional al fomentar la cooperación y la interacción social entre los estudiantes de medicina y los prepara aún más para su futuro papel como educadores de ciencias médicas. Se justifica la realización de más investigaciones sobre el impacto de la AAP en el rendimiento académico, tanto a corto como a largo plazo, en los programas de medicina.

Resumo Introdução: A Aprendizagem Assistida por Pares (AAP) é um método de ensino que desenvolve novos conhecimentos e competências através do apoio ativo à aprendizagem por pares. Objetivos: Avaliar o impacto do AAP na educação médica, recolhendo dados quantitativos que exploram a sua eficácia. Avaliar a perceção e a satisfação dos estudantes com AAP. Métodos: Foi desenvolvido um inquérito à escala Likert de 5 pontos para estudantes participantes no programa AAP, quer como tutores, quer como alunos. Os dados foram comparados e analisados estatisticamente de acordo com o género, GPA, e ano de estudo. Além disso, o acompanhamento dos tutores e dos alunos foi feito através da comparação das suas notas com as que não foram partilhadas no AAP e com o seu GPA do ano anterior. Resultados: A análise estatística dos dados do questionário revelou que os estudantes de medicina consideraram o ensino voluntário por pares semelhante ao ensino docente e têm uma perceção geralmente positiva do AAP. A maioria dos inquiridos relatou ter beneficiado tanto profissionalmente como pessoalmente das atividades de AAP. Recomendaram a expansão do papel do AAP e a incorporação oficial das sessões AAP no currículo. A realização dos estudantes foi significativamente aumentada para aqueles que atuaram como alunos e tutores de pares. Conclusões: A utilização do AAP em conjunto com outros métodos de ensino e aprendizagem proporciona um valor adicional ao promover a cooperação e interação social entre estudantes de medicina e prepara-os ainda mais para o seu futuro papel como educadores de ciências médicas. É necessária investigação adicional sobre o impacto do AAP nos resultados académicos, tanto a curto como a longo prazo, em programas médicos.

Abstract Introduction: Peer-assisted learning (PAL) is a teaching method that develops new knowledge and skills through active learning support from peers. Objectives: To assess the impact of PAL in medical education, by collecting quantitative data that explore its effectiveness. To evaluate students’ perception of and satisfaction with PAL. Methods: A 5-point Likert scale survey was developed for students participating in the PAL program either as tutors or learners. Data were compared and statistically analyzed according to gender, GPA, and year of study. Moreover, follow up of peer tutors and peer learner’s achievement was done through comparing their grades with those that not shared in PAL and with their GPA of previous year. Results: Statistical analysis of questionnaire data revealed that medical students regarded voluntary peer-teaching similarly to faculty teaching and have a generally positive perception of PAL. Most of the respondents reported that they benefited both professionally and personally from PAL activities. They recommended expanding the role of PAL and officially incorporating PAL sessions into the curriculum. Students’ achievement was significantly increased for those who acted as peer learners and peer tutors. Conclusions: Using PAL in conjunction with other teaching and learning methods provides additional value by fostering cooperation and social interaction among medical students and further prepares them for their future role as medical science educators. Additional research into the impact of PAL on academic achievement, both short- and long-term learning outcomes, in medical programs is warranted.

Three simple methods are described for the determination of amoxicillin (AMX) in bulk drug and dosage forms using iodate-iodide mixture reagent. These methods employed the well-known analytical reaction between iodate-iodide mixture in the presence of acid solution. The iodide oxidized by iodate to iodine in an amount equivalent to the -COOH group present in amoxicillin (AMX) and the liberated iodine determined by titrimetry and spectrophotometry. In the titrimetric method (method A), the liberated iodine was reacted with a measured excess of thiosulphate and the residual thiosulphate was determined by titration with a standard iodine solution using starch indicator, the method was applicable over 2 - 16 mg of AMX. In the second and third methods, the absorbance of the liberated iodine was measured at 370 nm (method B) and also iodine was reacted with starch and starch-iodine complex was measured at 570 nm (method C). In spectrophotometric methods (B and C), Beer’s law was obeyed over the concentration range of 5 - 50 and 5 - 60 µg mL-1 AMX with a molar absorptivity values of 5.55×103 L mol-1 cm-1 and 4.76×103 L mol-1 cm-1 for the method A and method B, respectively. The limits of detection (LOD) and quantification (LOQ) were calculated for method B and method C. The proposed methods were found to yield reliable results when applied to bulk drug and dosage forms analysis, and hence they can be applied in quality control laboratories.

Resumo Três bactérias solubilizantes de fosfato foram isoladas e identificadas por seqüenciamento de rRNA 16S como Pseudomonas putida, Pseudomonas sp e Pseudomonas fulva. As estirpes foram submetidas a testes bioquímicos de plantas e todos os atributos PGPR foram verificados na presença de pesticidas (clorpirifos e piriproxifeno). O índice de solubilização de fosfato da estirpe Ros2 foi mais elevado no meio NBRIP, isto é, 2,23 mm. Todas as estirpes apresentaram um pH ácido (varia de 2,5-5) em ambos os meios, isto é PVK e NBRIP. A estirpe Ros2 foi altamente positiva para a produção de amoníaco, bem como a produção de sideróforos enquanto a estirpe Rad2 foi positiva para a produção de HCN. Os resultados obtidos pelas estirpes Rad1, Rad2 e Ros2 para a produção de auxina foram 33,1, 30,67 e 15,38 μg ml-1 , respectivamente. A deformação Rad1 mostrou aumento de 16% na germinação percentual em comparação com o controlo na presença de stress de pesticida. Os resultados mais promissores para a estimativa do teor de clorofila foram obtidos na presença de carotenóides até 6 mgg-1 sem estresse por ambas as cepas Rad1 e Rad2. Estudo sugere que especialmente a estirpe Ros2 pode melhorar parâmetros de crescimento de plantas no estresse de pesticidas.

Abstract Three phosphate solubilizing bacteria were isolated and identified by 16S rRNA sequencing as Pseudomonas putida, Pseudomonas sp and Pseudomonas fulva . The strains were subjected to plant biochemical testing and all the PGPR attributes were checked in the presence of pesticides (chlorpyrifos and pyriproxyfen). The phosphate solubilizing index of strain Ros2 was highest in NBRIP medium i.e 2.23 mm. All the strains showed acidic pH (ranges from 2.5-5) on both medium i.e PVK and NBRIP. Strain Ros2 was highly positive for ammonia production as well as siderophore production while strain Rad2 was positive for HCN production. The results obtained by the strains Rad1, Rad2 and Ros2 for auxin production were 33.1, 30.67 and 15.38 µg ml-1, respectively. Strain Rad1 showed 16% increase in percentage germination in comparison to control in the presence of pesticide stress. Most promising results for chlorophyll content estimation were obtained in the presence of carotenoids upto 6 mgg-1 without stress by both strains Rad1 and Rad2. Study suggests that especially strain Ros2 can enhance plant growth parameters in the pesticide stress.

Abstract Due to their robustness in handling the inherent singularity difficulties associated with crack analysis, mesh-reduction methods present an avalanche of formulations in the literature which, sometimes, entails modifications to their conventional/standard forms for better results. Although such formulations provide a pool of alternative choices to the analyst, increase in their number requires some relative assessment between them in order to guarantee optimum choice of analysis tool. The present study assesses the applicability and relative performance of three such mesh-reduction methods, namely the radial basis function (RBF) method, the boundary element method (BEM), and the method of fundamental solution (MFS) for mode III crack analysis. In order to have a common ground for performance comparison, these methods are, first, tested in their most basic forms and simplest conventional formulations possible. Failure of some of them to provide reliable results calls for some enrichments. Yet, unless where necessary, efforts are made to ensure that unnecessary computationally expensive formulations are avoided. Consequently, the BEM formulation is not altered in any way, and modifications to both the RBF and MFS are limited to enrichment by the addition of, at most, one singular term and/or the domain-decomposition technique. Verification is achieved using the literature results and/or those obtained by FEM in this study. Summary of the relative advantages and limitations of the methods for mode III crack analysis is given to serve as a yard-stick based on which the choice of one over the others may be influenced.

ABSTRACT Forty male Naemi lambs were used to evaluate the effect of total mixed rations (TMR) alone or in combination with supplementation of alfalfa hay offered at different schedules on growth, carcass characteristics, and meat quality. Lambs at the age of three months, with an average weight of 28.85±1.09 kg, were randomly selected and distributed into four different feeding systems, 10 lambs each, as follows: TMR diet (control); TMR plus 100 g alfalfa hay; TMR plus 200 g alfalfa hay every two days; and TMR plus 300 g alfalfa hay every three days. The TMR and fresh drinking water were offered ad libitum. Lambs in the treated groups (with alfalfa hay) had significantly higher body weight, body weight change, dry matter intake, and feed conversion ratio compared with the control. Similarly, slaughter weight, carcass weight (hot and cold), and internal organs weight significantly increased in the treated groups, except for dressing weight percentage, which improved only in the treatment with TMR plus 300 g alfalfa hay. By contrast, mesentery fat, back fat, and body wall fat decreased in treated groups. Meat quality characteristics indicated that cooking loss and pH values did not differ between the control and treated groups; however, meat colour (lightness, yellowness, and redness) and pH (1 and 24 h) improved in post-slaughter treated groups. Furthermore, no difference was found in texture profile analysis (hardness, springiness, cohesiveness, and chewiness), although the shear force decreased significantly in treated groups. Addition of alfalfa hay offered at the present plan may not only improve the growth and carcass characteristics, but also enhance the meat quality of Naemi lambs. From the labour and economic point of view, TMR plus 300 g alfalfa hay every three days may be adopted while taking into account the management decision.

BACKGROUND: Despite manifold benefits of nanoparticles (NPs), less information on the risks of NPs to human health and environment has been studied. Cobalt oxide nanoparticles (Co3O4-NPs) have been reported to cause toxicity in several organisms. In this study, we have investigated the role of Co3O4-NPs in inducing phytotoxicity, cellular DNA damage and apoptosis in eggplant (Solanum melongena L. cv. Violetta lunga 2). To the best of our knowledge, this is the first report on Co3O4-NPs showing phytotoxicity in eggplant. RESULTS: The data revealed that eggplant seeds treated with Co3O4-NPs for 2 h at a concentration of 1.0 mg/ml retarded root length by 81.5 % upon 7 days incubation in a moist chamber. Ultrastructural analysis by transmission electron microscopy (TEM) demonstrated the uptake and translocation of Co3O4-NPs into the cytoplasm. Intracellular presence of Co3O4-NPs triggered subcellular changes such as degeneration of mitochondrial cristae, abundance of peroxisomes and excessive vacuolization. Flow cytometric analysis of Co3O4-NPs (1.0 mg/ml) treated root protoplasts revealed 157, 282 and 178 % increase in reactive oxygen species (ROS), membrane potential (APm) and nitric oxide (NO), respectively. Besides, the esterase activity in treated protoplasts was also found compromised. About 2.4-fold greater level of DNA damage, as compared to untreated control was observed in Comet assay, and 73.2 % of Co3O4-NPs treated cells appeared apoptotic in flow cytometry based cell cycle analysis. CONCLUSION: This study demonstrate the phytotoxic potential of Co3O4-NPs in terms of reduction in seed germination, root growth, greater level of DNA and mitochondrial damage, oxidative stress and cell death in eggplant. The data generated from this study will provide a strong background to draw attention on Co3O4-NPs environmental hazards to vegetable crops.

Contamination of ground-water from point and non-point sources is one of the major problems of water resources in Jordan. Altogether one hundred and six groundwater samples were collected from twenty six karst springs emerging from Umm Rijam aquifer and three wells tapping Amman-Wadi As Sir aquifer, and investigated for NO3- concentrations. Results showed that NO3- concentration in spring water ranged from 8 to 192 mg/L with an average of 33 mg/L. Seventy seven percent of the samples collected from the springs had nitrate concentrations exceeding the threshold value of 20 mg/L of anthropogenic source, and eight percent of the samples collected had nitrate concentrations higher than 50 mg/L, the maximum acceptable nitrate concentration for drinking water. About eighty percent of the sampled springs had nitrate concentrations higher than 20 mg/L. The K-means cluster analysis performed on the collected samples revealed the presence of three major clusters. The data were processed for the possible presence ofdiscordant outliers using the unpublished computer program UDASYS by Verma and Díaz-González. There is a wide spatial variation in the nitrate concentration in spring water. Monitoring the water quality of these springs showed that the lowest concentrations of nitrate were found in the wet season (January, February, and December), while the highest concentrations were found in the dry season (August, September). Nitrate concentration in Amman-Wadi As Sir aquifer ranges from <1 mg/L to 19.2 mg/L, with an average of 9.8 mg/L. Untreated domestic wastewater is most probably the major source of the nitrate in the spring water, as the study area is not served with sewer system, and domestic wastewater is collected in cesspools dug in the karised Umm Rijam Formation. Moreover, in the area under consideration there are no major industries or intensive agricultural activities. The results of this study are useful to highlight one of the most important environmental problems, namely the degradation of the water quality, and may serve to alert and encourage local and national authorities to take substantial steps and actions to protect and manage water quality.

La contaminación del agua subterránea por fuentes puntuales y no puntuales es uno de los mayores problemas para los recursos de agua en Jordania. Se colectaron 106 muestras de agua subterránea de 26 manantiales cársticos que emanan del acuífero Umm Rijam y de tres pozos que drenan el acuífero Amman-Wadi As Sir, y se investigaron sus concentraciones de NO3-. Los resultados mostraron que la concentración de NO3- en agua de manantiales está en el rango de 8 mg/L a 192 mg/L, con un promedio de 33 mg/L. Setenta y siete por ciento de las muestras colectadas en manantiales tuvieron concentraciones de nitrato que exceden el valor límite de 20 mg/L de fuentes antropogénicas, y ocho por ciento de las muestras colectadas tuvieron concentraciones de nitrato mayores que 50 mg/L, la concentración máxima de nitrato permisible para agua potable. Aproximadamente el 8% de los manantiales muestreados tuvieron concentraciones de nitrato mayores que 20 mg/L. El análisis de conglomerados por el método de los centroides ('K-means cluster analysis') reveló la presencia de tres conglomerados mayores. Los datos fueron analizados por la posible presencia de valores discordantes empleando el programa de cómputo no publicado UDASYS de Verma y Díaz-González. Existe una amplia variación espacial en la concentración de nitrato en agua de manantial. El monitoreo de la calidad del agua de esos manantiales mostró que las concentraciones más bajas de nitrato se encontraron en la estación de lluvias (enero, febrero y diciembre), mientras que las concentraciones más altas se encontraron en la estación seca (agosto y septiembre). La concentración de nitrato en el acuífero Amman-Wadi As Sir varía entre <1 mg/L y 19.2 mg/L, con un promedio de 9.8 mg/L. La fuente más probable de nitrato en los manantiales son las aguas residuales domésticas no tratadas, ya que el área de estudio no cuenta con sistema de drenaje y las aguas residuales domésticas son colectadas en cisternas cavadas en la Formación Umm Rijam afectada por procesos cársticos. Además, en el área no hay industrias grandes o actividades agrícolas intensivas. Los resultados de este estudio son útiles para resaltar uno de los problemas ambientales más importantes como es la degradación de la calidad del agua, y pueden servir como una alerta a las autoridades locales y nacionales para que sean tomados pasos substanciales y acciones para proteger y manejar la calidad del agua.