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Abstract The objective of this study was to formulate an experimental light-cured periodontal dressing containing alpha-humulene and to compare its physical, antimicrobial, and cytotoxicity properties with commercial gold standards (Barricaid® and Periobond®). Two periodontal dressing formulations were developed (a and b). The formulations were divided into 5 groups according to the alpha-humulene concentration as follows: Ea - control group, Ea1 - 1%, Ea5 - 5%, Ea10 - 10%, and Ea20 - 20%; Eb - control group, Eb1 - 1%, Eb5 - 5%, Eb10 - 10%, and Eb20 - 20%. Materials characterization was performed using the degree of conversion, cohesive strength, sorption, and solubility assays. Antimicrobial assay was performed using the modified direct contact test against E. faecalis and S. aureus. Cytotoxicity was assessed by the cell viability experiment using L929 fibroblasts. In general, the cohesive strength values of materials decreased as the alpha-humulene concentration increased. All the experimental dressings showed antimicrobial activity against both bacteria tested. Cell viability results for the Ea, Ea1, Eb, and Eb1 groups showed moderate cytotoxic effect. The formulations containing alpha-humulene showed similar behavior to the commercial references. Thus, formulations containing alpha-humulene have potential to be used as periodontal dressing.

https://doi.org/10.1590/1807-3107bor-2022.vol36.0091

Influence of EDTA and its Association with Benzalkonium Chloride on Enterococcus faecalis Adhesion to Dentin
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Titato, Pedro Cesar Gomes

; Zancan, Rafaela Fernandes

; Pedrinha, Victor Feliz

; Andrade, Flaviana Bombarda de

; Vivan, Rodrigo Ricci

; Duarte, Marco Antonio Hungaro

International journal of odontostomatology

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dec 2020, Volume 14 N. 4 Pages 632 - 638

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT: The aim of this in vitro study was to investigate the influence of ethylenediaminetetraacetic acid (EDTA) associated with the benzalkonium chloride (BAK) on the adhesion and formation of Enterococcus faecalis biofilms attached to coated dentin. Discs standard bovine dentin blocks were treated with the coating materials evaluated: Saline solution (control), 17 % EDTA, 17 % EDTA associated with 1 % BAK for 5 minutes and subsequently washed with saline solution. Afterwards, biofilms of E. faecalis (ATCC 29212) were grown on the surface of coated dentin blocks for time intervals of 1 hour and 7 days (n = 20) and were subsequently washed with phosphate-buffered saline (PBS). Bacterial viability and total biovolume were analyzed by confocal laser scanning microscopy (CLSM) using the Live/Dead technique. Nonparametric Kruskal-Wallis followed by Dunn tests were used to determine statistical differences (a = 5 %). The 17 % EDTA + 1 % BAK group showed significantly lower biovolume and bacterial viability values at the end of 1 hour (p < 0.05). After 7 days of contamination, the 17 % EDTA and 17 % EDTA + 1 % BAK groups showed similar results that differed statistically from those of the control group (p < 0.05). The saline solution group showed higher values. The use of BAK associated with EDTA on dentin blocks surfaces before exposure to contamination was able to interfere in the adhesion of E. faecalis to dentin. Also, dentin treatment by BAK associated with a chelating agent influences the secondary biofilm formation, which could have important effects on the long-term success of root canal treatment.

RESUMEN: El objetivo del estudio consistió en investigar in vitro, la influencia del ácido etilendiamino-tetraacético (EDTA) con cloruro de benzalconio (BAK) en la adhesión y formación de biopelículas de Enterococcus faecalis a la dentina. Discos de dentina bovina fueron tratadas con solución salina (control), 17 % de EDTA, 17% de EDTA asociado con 1 % de BAK durante 5 minutos y lavadas con solución salina. Las biopelículas de E. faecalis (ATCC 29212) se cultivaron sobre los discos de dentina durante intervalos de tiempo de 1 hora y 7 días (n = 20), lavados con solución salina tamponada con fosfato (PBS). La viabilidad bacteriana y el biovolumen total se analizaron mediante microscopía de barrido por láser (CLSM) utilizando la técnica Live / Dead. Se realizó prueba no paramétrica de Kruskal-Wallis, seguida por Dunn con una diferencia estadística (a = 5 %). El grupo de 17 % EDTA + 1 % BAK mostró valores significativamente menores de biovolumen y viabilidad bacteriana al final de 1 hora (p < 0,05). Después de 7 días de contaminación, los grupos de 17 % EDTA y 17 % EDTA + 1 % BAK mostraron resultados similares que diferían estadísticamente del grupo control (p < 0,05). La solución salina mostró valores más altos. La asociación de BAK con EDTA antes de la contaminación interfirió en la adhesión de E. faecalis. Además, el tratamiento de la dentina por BAK asociado con EDTA influye en la formación de biopelículas secundarias, lo que podría tener efectos importantes sobre el éxito a largo plazo del tratamiento del conducto radicular.

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Características biométricas e fisiológicas de sementes de Echium plantagineumL. em diferentes épocas de colheita
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Roso, Rodrigo

; Nunes, Ubirajara Russi

; Müller, Caren Alessandra

; Paranhos, Juçara Terezinha

; Dalcin, Joner Silveira

; Fernandes, Tiéle Stuker

; Forte, César Tiago

Revista de Ciências Agrárias

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dec 2019, Volume 42 N. 4 Pages 161 - 170

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O objetivo deste trabalho consistiu em avaliar as características biométricas e fisiológicas de diásporos (carcerulídeo: fruto concrescido com a semente) de Echium plantagineum colhidas em diferentes épocas, e a influência do armazenamento dos diásporos na germinação e viabilidade das sementes. Utilizou-se um fatorial 5 x 2 para avaliar as épocas de colheita dos frutos (03/out, 18/out, 01/nov, 15/nov e 01/dez) e tratamento de quebra da dormência (presença ou ausência). Os diásporos foram submetidos a avaliação da qualidade fisiológica, através de testes de germinação, índice de velocidade de germinação e viabilidade das sementes após cada colheita e depois do armazenamento durante seis meses, bem como a caracterização biométrica dos mesmos. Os frutos produzidos no início do período reprodutivo apresentam maiores dimensões e maior massa, ocorrendo dormência das sementes antes da dispersão do fruto. Ocorre variação na germinação das sementes de E. plantagineumno decorrer das épocas de colheita dos frutos, podendo estar relacionada com fatores genéticos e ambientais. A dormência das sementes é quebrada com o armazenamento durante seis meses e a imersão em nitrato de potássio (0,2%) durante 12-h e ácido giberélico (500 mg L-1) durante 48-h.

The objective of this work was to evaluate the biometric and physiological characteristics of diaspores (carcerulide: seeded seed fruit) of Echium plantagineum harvested at different times, and the influence of diaspores storage on seed germination and viability. A 5 x 2 factorial was used to evaluate fruit harvesting times (03/Oct, 18/Oct, 01/Nov, 15/Nov and 01/Dec) and dormancy break treatment (presence or absence). The diaspores were submitted to physiological quality evaluation through tests of germination, germination speed index and seed viability after each harvested and after storage for six months, as well as their biometric characterization. The fruits produced at the beginning of the reproductive period present have larger dimensions and larger mass, occurring seeds dormancy before fruit dispersal. There is variation in the germination of E. plantagineum seeds during the fruit harvesting periods, which may be related to genetic and environmental factors. Seed dormancy is broken with storage for six months and immersion in potassium nitrate (0.2%) for 12-h and gibberellic acid (500 mg L-1) for 48-h.

https://doi.org/10.19084/rca.18099 365 downloads

Physicochemical and microbiological characterization and antioxidant capacity of açaí pulps marketed in the states of Minas Gerais and Pará, Brazil
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Coutinho, Rosemary Maria Pimentel

; Fontes, Edimar Aparecida Filomeno

; Vieira, Luciana Marques

; Barros, Frederico Augusto Ribeiro de

; Carvalho, Antonio Fernandes de

; Stringheta, Paulo César

Ciência Rural

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2017, Volume 47 N. 1 elocation e20151172

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O mercado de polpa de açaí ( Euterpe oleracea Mart.) congelada tem tido crescimento aceitável e apresenta grande potencial mercadológico em função da sazonalidade e do elevado nível nutricional do fruto. Porém, devido à inexistência de padrões para todos os tipos de frutas, encontram-se no mercado produtos sem uniformidade. O objetivo do presente estudo foi avaliar polpas de açaí congeladas de diferentes marcas comerciais, quanto às características químicas, físico-químicas e microbiológicas. Para ATT e pH, duas marcas do Pará e duas de Minas Gerais foram reprovadas, quando comparadas com a legislação vigente. Observou-se que K (1253,6mg 100g-1), Ca (312mg 100g-1), Mg (178mg 100g-1), e P (145mg 100g-1) foram os principais minerais encontrados nas amostras avaliadas. O Fe apresentou conteúdo médio entre 9,65mg 100g-1 e 22,66mg 100g-1. Quanto às características microbiológicas, as contagens para E.coli atenderam à legislação. As contagens de mesófilos aeróbios, fungos filamentosos e leveduras apresentaram variações nos resultados. A contagem de S. aureus coagulase positiva apresentou-se dentro dos padrões (102UFC g-1) e foi detectada a presença de Salmonella sp. em uma marca, dentre as seis avaliadas. As polpas de açaí analisadas apresentaram valores de fenólicos totais variando de 240,14mg AGE 100g-1 a 372,43mg AGE 100g-1 e os níveis de antocianinas totais variando de 12,05 a 24,98mg 100g-1. Os resultados físico-químicos e microbiológicos obtidos indicaram a necessidade de implementação imediata das Boas Práticas de Fabricação; em contrapartida, as polpas congeladas de açaí apresentaram considerável potencial antioxidante.

ABSTRACT: The frozen açaí ( Euterpe oleracea Mart.) pulp market has had acceptable development and presents great market potential due to its high nutritional level and fruit's seasonality. However, due to the lack of standards for all types of fruit, products without uniformity are in the market. This study aimed to evaluate frozen açaí pulp belonging to different trademarks related to chemical, physicochemical, and microbiological characteristics. Regarding the total titratable acidity (TTA) and pH, two trademarks from Pará and two from Minas Gerais were rejected compared with the current legislation. It was observed that K (1,253.6mg 100 g-1), Ca (312mg 100g-1), Mg (178mg 100g-1), and P (145mg 100g-1) were the main minerals reported in the analyzed samples. Fe showed an average content from 9.65mg 100g-1 to 22.66mg 100g-1. As for microbiological characteristics, the counts for E. coli were in compliance with legislation. Counts of mesophilic aerobic, filamentous fungi, and yeasts showed variations in the results. The count of coagulase-positive S. aureus met the standards [102 Colony Forming Units (CFU) g-1]; and presence of Salmonella sp. in a trademark was detected among the six evaluated trademarks. The analyzed pulp of açaí showed total phenolics values ranging from 240.14mg AGE 100g-1 to 372.43mg AGE 100g-1, and anthocyanin total levels ranging from 12.05 to 24.98mg 100g-1. The obtained physicochemical and microbiological results indicated the need for immediate implementation of Good Manufacturing Practices; conversely, the frozen pulp of açaí showed considerable antioxidant potential.

https://doi.org/10.1590/0103-8478cr20151172 2916 downloads

Características do parto e involução uterina em ovelhas nativas do Pantanal brasileiro
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Fernandes, Carlos E.

; Cigerza, Cristiane Fabiane

; Pinto, Guilherme dos Santos

; Miazi, Cesar

; Barbosa-Ferreira, Marcos

; Martins, Charles Ferreira

Ciência Animal Brasileira

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jun 2013, Volume 14 N. 2 Pages 245 - 252

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O objetivo deste estudo foi descrever aspectos do parto e da involução uterina em ovelhas nativas do Pantanal. Foram estudadas 57 fêmeas pluríparas com cio sincronizado. Ao parto, avaliou-se sua classificação (simples ou gemelar), o tempo de expulsão da placenta, peso da placenta, total de diâmetro dos cotilédones, além do sexo e peso do cordeiro. A involução uterina foi avaliada pelo perfil leucocitário e de células de descamação (esfregaços cérvico-uterinos) e pela ultrassonografia uterina transretal nos dias 1, 7, 14, 21 e 28 dias pós-parto. O peso da placenta foi superior (P<0,05) nas fêmeas nascidas tanto dos partos simples quanto nos gemelares seguido pelo total de cotilédones. O tempo de expulsão da placenta e diâmetro dos cotilédones não diferiu (P<0,05) entre o sexo e parto. Os períodos 1, 7 e 14 dias pós-parto apresentaram valores superiores (P<0,05) para neutrófilos nos partos gemelares (78,5±9,5; 58,5±9,6 e 31,9±9,8, respectivamente). Macrófagos diferiram (P<0,05) nos períodos 1 e 14 para fêmeas com partos gemelares (12,5±1,6 e 6,7±1,6, respectivamente). A análise da regressão revelou ajuste quadrático (P<0,001) para o diâmetro, área e volume uterinos considerando todo o período pós-parto. As características observadas ao parto nas ovelhas nativas são semelhantes a ovelhas de outras raças. O perfil leucocitário e análise ultrassonográfica sugerem que a involução uterina nas ovelhas nativas do Pantanal varia entre 14 e 21 dias após o parto.

The aim of this study was to describe aspects of the parturition and uterine involution in native sheep from Brazilian Pantanal. Fifty-seven pluriparous ewes were used in this study. At parturition, the placenta expulsion (h), weight (g), total diameter of cotyledons, sex and weight of newborn, and single or twin parturition were evaluated. The uterine involution was evaluated by leucocytes profile, desquamation cells (cervix-uterine smears) and transrectal ultrasonography at 1, 7, 14, 21 and 28 days post-partum. The weight of the placenta and total of cotyledons were higher (P<0.05) in female lambs from single and twin parturition. Placental expulsion time and cotyledons diameter were not different (P>0.05) between sex and parturition classification. The total of neutrophils in twin parturitions were higher in days 1, 7 and 14 postpartum (78.5±9.5; 58.5±9.6 and 31.9±9.8, respectively). Macrophages were different (P<0.05) at 1 and 14 days postpartum in ewes with twin parturition (12.5±1.6 and 6.7±1.6, respectively). The regression analysis model revealed a quadratic curve estimation (P<0.001) to the diameter, area and uterine volume considering all postpartum period. In conclusion, the parturition characteristics are similar to other breeds. Leukocytary profile and the transrectal ultrasonography exam suggest that the uterine involution in native sheep of Brazilian Pantanal varies among 14 and 21 days postpartum.

https://doi.org/10.5216/cab.v14i2.17926 3140 downloads

Atividade inseticida de óleos essenciais de Pelargonium graveolens l'Herit e Lippia alba (Mill) N. E. Brown sobre Spodoptera frugiperda (J. E. Smith)
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Niculau, Edenilson dos S.

; Alves, Péricles B.

; Nogueira, Paulo Cesar de L.

; Moraes, Valéria Regina de S.

; Matos, Andréia P.

; Bernardo, Antonio R.

; Volante, Ana C.

; Fernandes, João B.

; Silva, Maria F. G. F. da

; Corrêa, Arlene G.

; Blank, Arie F.

; Silva, Anderson de C.

; Ribeiro, Leandro do P.

Química Nova

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2013, Volume 36 N. 9 Pages 1391 - 1394

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Insecticidal activity of essential oils of Pelargonium graveolens, Lippia alba and compounds geraniol, linalool, 1,8-cineole, limonene, carvone, citral and Azamax® were evaluated against Spodoptera frugiperda. Topical application assay showed essential oil of P. graveolens has acute toxicity against Spodoptera frugiperda larvae (third instar) with LD50 1.13 µg/mg per insect and LD90 2.56 µg/mg per insect. Three essential oils of L. alba also exhibited insecticidal activity with LD50 ranging from 1.20 to 1.56 µg/mg per insect and LD90 from 2.60 to 3.75 µg/mg per insect. Geraniol, linalool, carvone and citral caused significant mortality of 30, 90, 84 and 64% respectively, compared to negative control. The bioinsecticide, Azamax®, caused lower mortality than the compounds of the essential oils.

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Echinococcus granulosus genotypes circulating in alpacas (Lama pacos) and pigs (Sus scrofa) from an endemic region in Peru
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Sánchez, Elizabeth

; Cáceres, Omar

; Náquira, César

; Miranda, Eduardo

; Samudio, Franklyn

; Fernandes, Octavio

Memórias do Instituto Oswaldo Cruz

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mar 2012, Volume 107 N. 2 Pages 275 - 278

Abstract: En | Text: En | PDF: En

The identification of the genotypes of Echinococcus granulosus present in livestock and wild animals within regions endemic for cystic echinococcosis (CE) is epidemiologically important. Individual strains display different biological characteristics that contribute to outbreaks of CE and that must be taken into account in the design of intervention programs. In this study, samples of hydatid cysts due to E. granulosus were collected from alpacas (4) in Puno and pigs (8) in Ayacucho in Peru, an endemic region for CE. Polymerase chain reaction amplification and DNA sequencing of specific regions of the mitochondrial cytochrome C oxidase subunit 1 and NADH dehydrogenase subunit 1 genes confirmed the presence of a strain common to sheep, the G1 genotype, in alpacas. Two different strains of E. granulosus were identified in pigs: the G1 and the G7 genotypes. This is the first report of the G1 genotype of E. granulosus in alpacas in endemic regions of CE in Peru.

3229 downloads Cited 1 time in SciELO

Chemical constituents of methanolic extracts of Jatropha curcas L and effects on Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)
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Ribeiro, Sandra Santos

; Silva, Thanany Brasil da

; Moraes, Valéria Regina de Souza

; Nogueira, Paulo Cesar de Lima

; Costa, Emmanoel Vilaça

; Bernardo, Antônio Rogério

; Matos, Andreia Pereira

; Fernandes, Batista

; Silva, Maria Fátima das Graças Fernandes da

; Pessoa, Ângela Maria dos Santos

; Silva-Mann, Renata

Química Nova

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2012, Volume 35 N. 11 Pages 2218 - 2221

Abstract: En | Text: En | PDF: En

The biological activity of seven extracts from leaves of different Jatropha curcas L (Euphorbiaceae) accessions was evaluated on Spodoptera frugiperda. Methanol extracts were incorporated into an artificial diet and offered to the larval stage of S. frugiperda. The parameters evaluated were length of larval and pupal stages, mortality of larval and total cycle stage, and weight of pupae. The extracts of the EMB accessions showed the best result for larval mortality at 60.00 and 56.67%, compared with the control, respectively. Hexane partition of the methanol extract of the leaves of PM-14 accessions allowed the identification of phytosterols, phytol and n-alkanols.

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10.

Molecular characterization of Echinococcus granulosusfrom Peru by sequencing of the mitochondrial cytochrome C oxidase subunit 1 gene
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Sánchez, Elizabeth

; Cáceres, Omar

; Náquira, César

; Garcia, David

; Patiño, Gladys

; Silvia, Herrera

; Volotão, Aline C

; Fernandes, Octavio

Memórias do Instituto Oswaldo Cruz

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sep 2010, Volume 105 N. 6 Pages 806 - 810

Abstract: En | Text: En | PDF: En

Echinococcus granulosus, the etiologic agent of cystic echinococcosis (CE) in humans and other animal species, is distributed worldwide. Ten intra-specific variants, or genotypes (G1-G10), have been defined based on genetic diversity. To determine the genotypes present in endemic areas of Peru, samples were collected from cattle (44), sheep (41) and humans (14) from Junín, Puno Huancavelica, Cusco, Arequipa and Ayacucho. DNA was extracted from protoscolex and/or germinal layers derived from 99 E. granulosus isolates and used as templates to amplify the mitochondrial cytochrome C oxidase subunit 1 gene. The resulting polymerase chain reaction products were sequenced and further examined by sequence analysis. All isolates, independent of the host, exhibited the G1 genotype. Phylogenetic analysis showed that three isolates from Ayacucho shared the same cluster with microvariant G1(4). The G1 genotype is considered the most widespread and infectious form of E. granulosusworldwide and our results confirm that the same patterns apply to this country. Therefore, these findings should be taken into consideration in developing prevention strategies and control programs for CE in Peru.

3732 downloads Cited 4 times in SciELO

11.

Prevenção de doenças cardiovasculares em mulheres climatéricas e a influência da terapia de reposição hormonal: uma diretriz baseada em evidências
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Gebara, Otavio C. E.

; Fernandes, Cesar

; Bertolami, Marcelo C.

Arquivos Brasileiros de Cardiologia

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nov 2009, Volume 93 N. 5 Pages e86 - e88

Abstract: Pt | Text: Pt | PDF: Pt

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12.

Oclusão percutânea do pequeno canal arterial com molas de gianturco: impacto da otimização da seleção das molas e dos pacientes e da não-tolerância ao fluxo residual significativo imediato nos resultados
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Pedra, Carlos Augusto Cardoso

; Esteves, César Augusto

; Braga, Sérgio Luiz Navarro

; Pedra, Simone Rolim Fernandes Fontes

; Pontes Júnior, Sérgio Cunha

; Silva, Maria Aparecida de Paula

; Almeida, Gisele de Montalvão e Alpuim Lousadas

; Santana, Maria Virgínia Tavares

; Fontes, Valmir Fernandes

Revista Brasileira de Cardiologia Invasiva

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2008, Volume 16 N. 1 Pages 86 - 90

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

INTRODUÇÃO: A oclusão percutânea do canal arterial persistente (PCA) com implante retrógrado não controlado de molas de Gianturco tem sido freqüentemente empregada. MÉTODO: Desde janeiro de 2001, temos aplicado essa técnica em pacientes com canais < 3 mm, dos tipos A, D e E, e não tolerado fluxos residuais imediatos significativos, implantando outra mola. Neste artigo, descrevemos os resultados desse tipo de abordagem. RESULTADOS: Desde janeiro de 2001, 178 pacientes (110 do sexo feminino; mediana de idade e peso: cinco anos e 17 kg, respectivamente) foram submetidos ao procedimento. A média do diâmetro mínimo do PCA foi de 1,8 ± 0,6 mm (0,5 mm a 3,0 mm), sendo 157 do tipo A, nove do tipo D e 12 do tipo E. Em três pacientes, houve embolização inicial das molas com resgate, sendo reimplantadas em dois (taxa de sucesso: 99,4%). Mais de uma mola foi utilizada em 32 (18%) pacientes, com canais significativamente maiores que os restantes (2,3 ± 0,5 mm vs. 1,6 ± 0,5 mm; p < 0,001). Oclusão imediata foi observada em 160 pacientes e 17 (9,6%) possuíam fluxo residual discreto, difuso e de baixa velocidade. Redução de pulsos e transfusão foram complicações observadas em dois pacientes. Um paciente com fluxo residual discreto não retornou. Todos os 176 pacientes, à ecocardiografia, apresentavam oclusão e nenhum apresentava distúrbios de fluxo na aorta ou na artéria pulmonar no seguimento (mediana: seis meses). CONCLUSÃO: A tolerância zero para fluxos residuais imediatos e a seleção otimizada das molas e dos pacientes, provavelmente, explicam esses ótimos resultados. O procedimento é simples, custo efetivo, seguro e altamente eficaz.

INTRODUCTION: Percutaneous occlusion of the patent ductus arteriosus (PDA) by non-controlled retrogade release of Gianturco coils has been frequently employed. METHODS: Since January of 2001, we have applied this technique for patients with type A, D and E PDAs with < 3 mm and have not tolerated significant immediate residual leaks, implanting additional coils. In this paper we report the outcome of this approach. RESULTS: From January 2001, 178 patients (110 females; median age and weight: 5 years and 17 kg, respectively) underwent the procedure. The mean minimal diameter of the PDA was 1.8 ± 0.6 mm (0.5 to 3.0 mm) with 157, 9 and 12 being of types A, D and E, respectively. In 3 patients, there was initial embolization with percutaneous retrieval followed by coil re-implantation in 2 (success rate: 99.4%). More than one coil was required in 32 patients with PDAs significantly larger than the remainder (2.3 ± 0.5 versus 1.6 ± 0.5 mm; p < 0.001). Immediate occlusion was observed in 160 patients with 17 (9.6%) having discrete, diffuse, low velocity residual leaks. Pulse reduction and transfusion were complications observed in 2 patients. One patient with mild residual leak was lost to follow up. On echocardiography, all 176 patients had occlusion and none had flow disturbances in the aorta or in the pulmonary artery on follow up (average 6 months). CONCLUSION: Zero tolerance for significant immediate residual leaks and optimized selection of coils and patients probably explain these excellent outcomes. The procedure is easy to perform, cost effective, safe and highly efficient.

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13.

Alcalóides de flores e folhas de Erythrina speciosa Andrews
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Faria, Terezinha de Jesus

; Cafêu, Mariana Carrara

; Akiyoshi, Gisele

; Ferreira, Dalva Trevisan

; Galão, Olívio Fernandes

; Andrei, César Cornélio

; Pinge Filho, Phileno

; Paiva, Manoel Ronaldo Carvalho

; Barbosa, Aneli de Melo

; Braz-Filho, Raimundo

Química Nova

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jun 2007, Volume 30 N. 3 Pages 525 - 527

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

In vitro bioassays with leave extracts of Erythrina speciosa showed promising activity against Trypanosoma cruzi. From the flowers of E. speciosa two alkaloids were isolated: erysotrine and erythartine. The leaves furnished one alkaloid, nororientaline. The structures were determined on the basis of spectroscopic evidence. This is the first report about the investigation of alkaloids in flowers and leaves of this species, as well the first report of nororientaline occurrence in this plant.

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14.

Uso de imagens do sensor ASTER na identificação de níveis de degradação em pastagens
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Nascimento, Melchior C.

; Riva, Rogério D. D.

; Chagas, César da S.

; Oliveira, Henrique de

; Dias, Luiz E.

; Fernandes Filho, Elpídio I.

; Soares, Vicente P.

Revista Brasileira de Engenharia Agrícola e Ambiental

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mar 2006, Volume 10 N. 1 Pages 196 - 202

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O manejo inadequado e a baixa disponibilidade de nutrientes nos solos da Zona da Mata de Minas Gerais, têm resultado em baixas produtividades de pastagens, aparecimento de solos descobertos e perdas de solo por erosão. O objetivo deste trabalho foi avaliar a capacidade das imagens do sensor ASTER em identificar diferentes níveis de degradação de pastagens. A área de estudo inclui parte dos municípios de Viçosa, Teixeiras e São Miguel do Anta, perfazendo cerca de 3.314 ha. Devido às características das pastagens da região, foram utilizados quatro níveis de degradação: leve, moderada, forte e muito forte. A classe que apresentou maior erro de classificação foi a pastagem com nível de degradação muito forte (Pastagem 4), com 53,91% dos pixels classificados, confundindo-se com as demais classes. A pastagem com nível de degradação moderada (Pastagem 2) apresentou a melhor classificação. Da área avaliada, aproximadamente 70% correspondem a pastagens, sendo 56,46% classificadas como fortemente degradadas; 28,73% Mata/Capoeira e 1,54% plantações de café. Os resultados permitiram concluir que as imagens do sensor ASTER apresentaram um potencial satisfatório para separar os diferentes níveis de degradação de pastagens.

The improper management and the low availability of nutrients of soils in "Zona da Mata" in Minas Gerais State, Brazil, have led to low productivity of natural pasture, emergence of bare soils and soil losses by erosion. The objective of this work was to evaluate the capacity of ASTER sensor images to identify different levels of degradation in pasture lands. The studied area includes part of Viçosa, Teixeiras, and São Miguel do Anta municipalities, forming a total area around 3,314 ha. Due to natural characteristics of the pasture in this region, four levels of degradation were used: light, moderate, strong, and very strong. The class that showed the highest error in the classification was the very strong, degraded with 53.91% of the classified pixels, not distinguishable from the other classes. The moderate degradation class showed the best classification. From the total evaluated area, approximately 70% corresponded to pasture, 56.46% of which was classified in the strong degradation level, 28.73% to 'Mata/Capoeira' and only 1.54% to coffee plantations. The results permit to conclude that the use of sensor ASTER images was satisfactory to separate degradation levels of pasture lands in the studied area.

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15.

Estudo de mecanismos e fatores relacionados com o abuso sexual em crianças e adolescentes do sexo feminino
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Drezett, Jefferson

; Caballero, Marcelo

; Juliano, Yara

; Prieto, Elizabeth T.

; Marques, José A.

; Fernandes, César E.

Jornal de Pediatria

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oct 2001, Volume 77 N. 5 Pages 413 - 419

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: estudar mecanismos e fatores relacionados com o abuso sexual, comparando suas freqüências entre vítimas crianças e adolescentes. MÉTODOS: estudo retrospectivo de 617 vítimas de abuso sexual atendidas entre julho de 1994 e agosto de 1999 pelo Centro de Referência da Saúde da Mulher; 71 crianças (idade < 10 anos) e 546 adolescentes (10 a 20 anos). As variáveis de estudo foram crime sexual; constrangimento; presunção de violência; tipificação do agressor; número de agressores; situação da vítima no momento do crime; e ocorrência de traumas físicos. Os dados foram informatizados em programa Epi Info 6 e os resultados analisados pelo teste de x². RESULTADOS: o estupro ocorreu em 90,8% das adolescentes e o atentado violento ao pudor em 46,5% das crianças. A violência presumida (VP) foi mais freqüente nas crianças (63,4%) e a grave ameaça nas adolescentes (63,2%). A inocencia consilli foi VP exclusiva entre crianças, atingindo 59,5% das adolescentes. Entre crianças, 84,5% foram abusadas por agressores identificáveis, geralmente do núcleo familiar, enquanto desconhecidos violentaram 72,3% das adolescentes. Nas crianças, o abuso ocorreu em 42,3% em suas residências e em 28,2% na do agressor. Adolescentes foram vitimadas durante atividades cotidianas (34,8%) e no percurso do trabalho ou escola (28,4%). A maioria das pacientes não apresentou traumas físicos genitais ou extragenitais. CONCLUSÕES: crianças foram submetidas, principalmente, ao atentado violento ao pudor, perpetrado por agressor conhecido, por meio de violência presumida e em ambientes privados. Nas adolescentes predominou o estupro por agressores desconhecidos, sob grave ameaça, em ambientes não domésticos.

OBJECTIVE: to study the frequency of sexual abuse in children and adolescents and its related factors. METHODS: retrospective analysis of 617 cases of sexual abuse, assisted between July 1994 and August 1999 at the Women's Health Reference Center, divided into two groups: 71 children (age <10 years), and 546 adolescents (age > or =10 and <20 years). The analyzed variables were: sexual crime; embarrassment; presumption of violence; characteristics and number of abusers; the victim's situation at the moment of the crime; and occurrence of physical traumas. Epi Info 6 software was utilized to gather data, and the results were analyzed by the chi-square test. RESULTS: significant statistic results were found. 90.8% of the adolescents were victims of rape, and 46.5% of the children suffered sexual assault. The presupposed violence (PV) was more frequent in the group of children (63.4%) and a serious threat in the group of adolescents (63.2%). Innocentia consilli was exclusive PV among 100% of children and 59.5% of adolescents. 84.5% of children were molested by identifiable abusers, most frequently family members. Perpetrators were unknown in 72.3% of the cases of adolescent abuse. 42.3% of sexual abuse of children occurred in their homes, and 28.2% at the abuser's. Adolescents were approached during daily activities (34.8%) and on their way to work or school (28.4%). Most patients did not have genital or extragenital trauma. CONCLUSIONS: children suffered sexual assault, perpetrated by a known abuser through presupposed violence in private places. Adolescents were raped by unknown individuals, under serious threat, in nondomestic places.

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