ABSTRACT Acute wound dressings can be based on PVA hydrogels, which present many characteristics of an ideal dressing, e.g., fluid uptake, a moisturized environment, etc. The lack of antimicrobial properties leads to the addition of natural active agents. The present work aims to manufacture and compare PVA gels loaded with Barbatimão bark extract, Leucaena bark extract, Aloe vera, and Lavender essential oil. They were characterized by FTIR, swelling tests, SEM, actives release. There were interactions between PVA and the active agents. The addition of Leucaena and Barbatimão increased the PVA ability to swell, but the opposite was found for Aloe vera and Lavender essential oil. PVA-Lavender essential oil samples presented interconnected pores, while samples with essential oil or extracts presented high crystallinity. Lavender essential oil and Aloe vera presented the highest release. The Barbatimão and Leucaena samples’ release may be related to the samples’ swelling, but the initial release of Aloe vera and Lavender samples was diffusion controlled by swelling. Their long-term release was dose-dependent for Aloe vera, while it was a non-Fickian diffusion for Lavender essential oil related to the hydrogel’s relaxation step. There is a synergistic effect when Aloe vera and Lavender essential oil are loaded in PVA hydrogels. hydrogels dressing eg e g e.g. uptake environment etc agents extract FTIR tests SEM swell PVALavender pores crystallinity longterm long term dosedependent dose dependent nonFickian non Fickian hydrogel s step e.g

RESUMO O controle biológico no Brasil vem crescendo devido aos problemas gerados pelo uso indiscriminado de inseticidas químicos. A Musca domestica representa o maior problema em granjas avícolas devido às condições favoráveis para seu crescimento populacional. Sendo assim, foram realizadas capturas de dípteros muscoides em um aviário na região de Montes Claros, MG, usando armadilhas contendo isca química e captura por busca direta e, destas moscas, foram isolados e identificados fungos residentes nestes insetos. Os fungos isolados foram duas espécies do gênero Aspergillus sp., um do gênero Memnoniella sp., Scopulariopsis sp., Paecilomyces sp e um fungo da família Moniliaceae. Foram também requeridos junto ao CENARGEM/EMBRAPA as espécies de fungos entomopatogênicos Beauveria bassiana CG 470 e CG 472; Metarhizium anisopliae CG 34 e CG 312 e o Paecilomyces sp. CG 301. As espécies selecionadas para os bioensaios foram um Aspergillus sp., Memnoniella sp. e os Metarhizium anisopliae CG 34 e CG 312 por terem boa esporulação. Os fungos Aspergillus sp. e o Memnoniella sp. não apresentaram capacidade entomopatogênica, os fungos M. anisopliae CG 34 e CG 312 foram bastante eficientes em controlar a emergência dos adultos de M. domestica, mostrando-se bons agentes de controle biológico.

ABSTRACT Use of biological control measures in Brazil has been increasing because of the problems generated from the indiscriminate use of chemical insecticides. Musca domestica represents the biggest problem to poultry farms due to the favorable conditions for its population growth. The present study was therefore conducted, beginning with captures of muscoid diptera on a poultry farm in the Montes Claros region, state of Minas Gerais, Brazil, using traps containing chemical bait and as well as capture by direct search, and the fungi resident on these flies was isolated and identified. The isolated fungi were two species of Aspergillus sp., one each from the genera Memnoniella sp., Scopulariopsis sp., Paecilomyces sp., and one fungi of the family Moniliaceae. Also, the entomopathogenic species of fungi Beauveria bassiana CG 470 and CG 472, Metarhizium anisopliae CG 34 and CG 312, and Paecilomyces sp. CG 301 were requested from CENARGEM/EMBRAPA. The species selected for the bioassays were Aspergillus sp., Memnoniella sp. and Metarhizium anisopliae CG 34 and CG 312. The fungi Aspergillus sp. and the Memnoniella sp. did not present entomopathogenic capacity; the fungi Metarhizium anisopliae CG 34 and CG 312 were sufficiently efficient in controlling the emergence of the adults of Musca domestica, showing themselves good agents of biological control.