RESUMO Objetivos: avaliar os níveis de estradiol e os efeitos do autotransplante de tecido ovariano aquecido, após vitrificação, no metabolismo ósseo de ratas previamente ooforectomizadas bilateralmente. Métodos: trabalho experimental com 27 ratas com idades entre 11 e 12 semanas e pesando 200g a 300g, submetidas à ooforectomia bilateral e criopreservação de tecido ovariano para posterior reimplante. Os animais foram divididos em dois grupos, A e B, com oito e 19 ratas, respectivamente. O autotransplante ocorreu em dois períodos de acordo com o tempo de castração: após uma semana, no grupo A, e após um mês no grupo B. Mensurações de estradiol sérico e análise histológica de ovário e tíbia foram feitos antes e após o período de ooforectomia (precoce ou tardio) e um mês após o reimplante. Resultados: nos grupos A e B, as espessuras corticais médias da tíbia foram 0,463±0,14mm (média±DP) na linha de base, 0,360±0,14mm após ooforectomia e 0,445±0,17mm em um mês após o reimplante (p<0,005). As médias trabeculares foram 0,050±0,08mm (média±DP) na linha de base, 0,022±0,08mm após ooforectomia e 0,049±0,032mm em um mês após o reimplante (p<0,005). Não houve diferença estatística entre a variação do estradiol entre os dois grupos de estudo (p=0,819). Conclusão: o transplante de tecido ovariano criopreservado restabeleceu os parâmetros ósseos, e estes resultados sugerem que a reimplantação ovariana em mulheres pode apresentar os mesmos efeitos benéficos sobre o metabolismo ósseo.

ABSTRACT Objectives: to evaluate estradiol levels and autotransplantation heated ovarian tissue effects, after vitrification, on rats bone metabolism previously oophorectomized bilaterally. Methods: experimental study with 27 rats aged 11 to 12 weeks and weighing 200g to 300g, submitted to bilateral oophorectomy and ovarian tissue cryopreservation for subsequent reimplantation. Animals were divided into two groups, A and B, with 8 and 19 rats, respectively. Autotransplantation occurred in two periods according to castration time: after one week, in group A, and after one month in group B. Serum estradiol measurements and ovary and tibia histological analysis were performed before and after oophorectomy period (early or late) and one month after reimplantation. Results: in groups A and B, tibia median cortical thickness was 0.463±0.14mm (mean±SD) at the baseline, 0.360±0.14mm after oophorectomy and 0.445±0.17mm one month after reimplantation p<0.005). Trabecular means were 0.050±0.08mm (mean±SD) at baseline, 0.022±0.08mm after oophorectomy and 0.049±0.032mm one month after replantation (p<0.005). There was no statistical difference in estradiol variation between the two study groups (p=0.819). Conclusion: cryopreserved ovarian tissue transplantation restored bone parameters, and these results suggest that ovarian reimplantation in women may have the same beneficial effects on bone metabolism.

Este ensaio aborda as produções artísticas de um coletivo colombiano chamado Mujeres Al Borde e busca descrever algumas de suas atividades e as maneiras como produzem e articulam relações entre arte, ativismo e produção audiovisual, abordando aí gênero, sexualidade e questões étnico-raciais. O trabalho trata das relações transnacionais desse coletivo com um grupo queer ativista estadunidense, Queer Women of Color Media Arts Project, para a criação de seu próprio programa de treinamento cinematográfico, a Escuela Audiovisual Al Borde; reflete sobre as possibilidades de pensarmos sobre ativismo queer no contexto latino-americano; e, por fim, analisa algumas das produções audiovisuais do Mujeres Al Borde, naquilo que compreendem como seu artivismo, neologismo que articula arte e ativismo. Enfocam-se as maneiras pelas quais esses coletivos produzem redes de colaboração transnacionais em contextos geopolíticos globalizados dissidentes, ao mesmo tempo em que buscam, através da relação que criam entre arte e política, promover transformação social e meios de expressão visual para comunidades LGBTQ, cultivando queer artivismos feministas no sul e no norte globais.

The present article analyzes the interactions between two collectives of activists/artists, the Queer Women of Color Media Arts Project (QWOCMAP), situated in the United States, and Mujeres Al Borde, situated in Colombia. By focusing on their interaction and addressing the audiovisual productions of the latter group, I discuss how they seek to create relationships between art, activism, audiovisual production, gender, sexualities, race/ethnicities, aiming to create transnational feminist coalitions. The article touches on the following topics: the creating process to developed a cinematic training program in partnership; reflects on the possibilities of thinking about activism and queer theories in Latin American context; and finally examines some of audiovisual productions of Mujeres Al Borde, trying to address what they call as their artivism, neologism that articulates art and activism. The focus here is on the ways in which these collectives are producing networks of transnational collaboration in global geopolitical dissidents' arenas, promoting social change and means of visual expression for LGBTQ communities, cultivating queer feminist social movements in the global north and global south.

OBJECTIVE: Oxidative stress plays an important role in skeletal muscle damage in sepsis. Aerobic exercise can decrease oxidative stress and enhance antioxidant defenses. Therefore, it was hypothesized that aerobic exercise training before a sepsis stimulus could attenuate skeletal muscle damage by modulating oxidative stress. Thus, the aim of this study was to evaluate the effects of aerobic physical preconditioning on the different mechanisms that are involved in sepsis-induced myopathy. METHODS: Male Wistar rats were randomly assigned to either the untrained or trained group. The exercise training protocol consisted of an eight-week treadmill program. After the training protocol, the animals from both groups were randomly assigned to either a sham group or a cecal ligation and perforation surgery group. Thus, the groups were as follows: sham, cecal ligation and perforation, sham trained, and cecal ligation and perforation trained. Five days after surgery, the animals were euthanized and their soleus and plantaris muscles were harvested. Fiber cross-sectional area, creatine kinase, thiobarbituric acid reactive species, carbonyl, catalase and superoxide dismutase activities were measured. RESULTS: The fiber cross-sectional area was smaller, and the creatine kinase, thiobarbituric acid reactive species and carbonyl levels were higher in both muscles in the cecal ligation and perforation group than in the sham and cecal ligation and perforation trained groups. The muscle superoxide dismutase activity was higher in the cecal ligation and perforation trained group than in the sham and cecal ligation and perforation groups. The muscle catalase activity was lower in the cecal ligation and perforation group than in the sham group. CONCLUSION: In summary, aerobic physical preconditioning prevents atrophy, lipid peroxidation and protein oxidation and improves superoxide dismutase activity in the skeletal muscles of septic rats.

O milho é uma planta C4 que apresenta pouca, ou nenhuma, resposta às elevadas [CO2]; assim, neste trabalho, objetivou-se analisar respostas fisiológicas e produtivas da cultura do milho sob alta [CO2], e desenvolver câmaras de topo aberto (CTA) para criar uma atmosfera enriquecida com CO2. O experimento foi conduzido entre outubro de 2008 e março de 2009. As CTAs foram desenvolvidas em esquema modular. Foram realizadas medições da taxa fotossintética, transpiração, condutância estomática, produção de grãos e matéria seca. O delineamento experimental foi em blocos casualizados, com quatro repetições e três tratamentos: P1 - plantas cultivadas em CTA a [CO2] de 700ppm; P2 - plantas cultivadas em CTAcom [CO2] ambiente; e P3 -plantas cultivadas em campo aberto, testemunhas. Os resultados obtidos foram submetidos à análise de variância e teste de Tukey (Pr<0,05). As câmaras reduzem em 25% a Radiação Fotossinteticamente Ativa e aumentam a temperatura do ar e das folhas, em relação ao ambiente externo. As plantas sob alta [CO2] (P1) apresentaram as maiores taxas fotossintéticas e as menores condutâncias estomáticas e transpiração. O peso total dos grãos (g) e a matéria seca da parte aérea (g) não apresentaram incrementos para P1, apesar das maiores taxas fotossintéticas.

Maize is a C4 plant that shows few or no response to high [CO2]. Thus, this study aimed to analyze the photosynthetic rate and yield of maize under high [CO2] and develop open-top chambers (OTC) to create an atmosphere enriched with CO2. The experiment was conducted between October 2008 and March 2009. The OTCs were developed in modular scheme. Measurement of photosynthetic rates, transpiration, stomata conductance, grain yield and dry matter were performed. The experimental design was randomized blocks with four replications and three treatments: P1 - plants grown in OTC with 700 ppm [CO2], P2 - plants grown in OTC with environmental [CO2], and P3 - control, cultivated in open field. The results were analyzed by ANOVA and Tukey's test (Pr< 0.05). The chambers can reduce by 25% the photosynthetically active radiation and increase the air and leaf temperatures. Plants under high [CO2] (P1) showed the highest photosynthetic rates and the lowest stomata conductance and transpiration. The total weight of grains (g) and dry mass of shoots (g) showed no increases for P1, despite their higher photosynthetic rates.

This study focuses on the geographic distribution of the snail of the genus Biomphalaria and evaluates its infectivity by Schistosoma mansoni in 5264 specimens collected in the municipality of Juiz de Fora, Minas Gerais, Brazil. Of the 31 locations studied, 6 were reservoirs, 11 rudimentary holding ponds, 7 irrigation ditches, 5 lakes, 1 ornamental pond, and 1 waterfall. Intermediate hosts were found only in the rudimentary ponds and ditches, which were 100% positive. Using morphological and molecular analysis techniques, B. tenagophila, B. peregrina, and B. straminea were identified. This is the first report of B. stramínea in the municipality, and evaluation of its infective potential revealed susceptibility of 25.4%. Although we did not find specimens of Biomphalaria infected by S. mansoni, the data obtained indicate the presence of intermediate hosts, especially in the irrigation ditches in Juiz de Fora, and their proximity to contaminated areas.

Resumo Com objetivo de avaliar os efeitos da salinidade do solo sobre a nutrição mineral da variedade de milho UFVM 100, conduziu-se um experimento em lisímetros de drenagem sob condições de casa de vegetação, montado em delineamento inteiramente casualizado com sete tratamentos, sendo um irrigado com água doce e sem lixiviação e seis irrigados com água salina de 1,2 dS m-1, com frações de lixiviação de 40, 30, 20, 15, 10 e 5% da lâmina de irrigação aplicada, e três repetições. Os efeitos da salinidade do solo sobre a nutrição mineral da cultura do milho foram avaliados, determinando-se o teor foliar dos macronutrientes aniônicos e suas relações com o Cl aos 120 dias após o plantio, na fase fenológica correspondente à maturação fisiológica. Na variedade avaliada, o aumento da salinidade do solo elevou os teores foliares de cloro e as relações Cl/N, Cl/P, Cl/S, seguidos da redução dos teores de nitrogênio, fósforo e enxofre.

Abstract Aiming at the evaluation of the soil salinity effects upon the mineral nutrition of the maize (UFVM 100 cv.), an experiment was conducted in drainage lysimeters, under greenhouse conditions. A completely randomized design was used with seven treatments: one irrigated with fresh water without leaching and six irrigated with saline water of 1.2 dS m-1 with leaching fractions of 40, 30, 20, 15, 10 and 5% of the irrigation depth applied, and three replicaticns. The effects of the soil salinity upon the mineral nutrition of the maize crop were evaluated, by determining the leaf content of the anionic macronutrients and their relationships with Cl at 120 days after planting, at the phenological phase corresponding to the physiological maturation. For the evaluated variety, the increase in the soil salinity elevated the leaf contents of chloride and the relations Cl/N, Cl/P and Cl/S, followed by reduction in the contents of nitrogen, phosphorus and sulfur.