Abstract Objective To describe the clinical, laboratory, and radiological characteristics, as well as the outcomes of children with MIS-C. Method Multicenter, prospective cohort study, conducted in 17 pediatric intensive care units in five states in Brazil, from March to July 2020. Patients from 1 month to 19 years who met the MIS-C diagnostic criteria were included consecutively. Results Fifty-six patients were included, with the following conditions: Kawasaki-like disease (n = 26), incomplete Kawasaki disease (n = 16), acute cardiac dysfunction (n = 10), toxic shock syndrome (n = 3), and macrophage activation syndrome (n = 1). Median age was 6.2 years (IQR 2.4−10.3), 70% were boys, 59% were non-whites, 20% had comorbidities, 48% reported a contact with COVID-19 cases, and 55% had a recent SARS-CoV-2 infection confirmed by RT-PCR and/or serology. Gastrointestinal symptoms were present in 71%, shock symptoms in 59%, and severe respiratory symptoms in less than 20%. -Dimer was increased in 80% and cardiac dysfunction markers in more than 75%. Treatment included immunoglobulin (89%); corticosteroids, antibiotics, and enoxaparin in about 50%; and oseltamivir and antifungal therapy in less than 10%. Only 11% needed invasive mechanical ventilation, with a median duration of five days (IQR 5–6.5). The median length of PICU stay was six days (IQR 5–11), and one death occurred (1.8%). Conclusions Most characteristics of the present MIS-C patients were similar to that of other cohorts. The present results may contribute to a broader understanding of SARS-CoV-2 infection in children and its short-term consequences. Long-term multidisciplinary follow-up is needed, since it is not known whether these patients will have chronic cardiac impairment or other sequelae.

RESUMO: Este estudo se propôs a isolar Pseudomonas spp. durante o processo de ordenha em dez fazendas com sistemas manuais e mecanizados, durante as estações seca e chuvosa, além de avaliar a homologia do DNA e seus padrões de distribuição entre os isolados, a fim de se determinar as principais fontes de contaminação do leite. Cento e sessenta e sete isolados de Pseudomonas spp. foram obtidos a partir de amostras de água, mãos de ordenhadores, tetos, teteiras, tanques de resfriamento e leite cru armazenado, sendo 85 e 82 pontos de amostragem em fazendas com sistemas de ordenha manual e mecânico, respectivamente. Diferença estatisticamente significativa foi encontrada entre os isolados observados entre a superfície dos tetos antes e após o pré-dipping (p=0,02), mas nenhuma diferença foi encontrada entre sistemas de ordenha ou estações (p>0,05). A possibilidade do mesmo padrão de Pseudomonas spp. estar distribuído em diferentes fazendas ou estações foi avaliada pela técnica de Polimorfismo do Tamanho de Fragmento Amplificado (AFLP). As mãos de ordenhadores, superfície dos tetos das vacas, teteiras e tanques de resfriamento foram associados com a contaminação do leite cru, demonstrando que independente do tipo de ordenha e estação, a higiene adequada de equipamentos, utensílios e mãos dos ordenhadores é essencial para evitar contaminação do leite, e consequentemente aumentar sua qualidade.

ABSTRACT: This study focused on isolating Pseudomonas spp. during milking process in ten dairy farms with manual and mechanical milking systems during dry and rainy seasons, and evaluating DNA homology and patterns of distribution between isolates, in order to identify main sources of milk contamination by Pseudomonas spp. A total of 167 isolates of Pseudomonas spp. were obtained from water, milkers’ hands, cows’ teats, teat cups, cooling tanks and raw milk. Bacteria of Pseudomonas spp. genus were isolated from 85 and 82 sampling points in dairy farms with manual and mechanical milking system, respectively. A significant difference (p=0.02) on Pseudomonas spp. isolation was observed among samples of surface of cows’ teats before and after pre-dipping, but no significant difference (p>0.05) was observed among milking systems or seasons. The possibility of the same Pseudomonas spp. patterns are distributed in different farms and seasons using Amplified Fragment Length Polymorphism (AFLP) technique was demonstrated. Milkers’ hands, surface of cows’ teats, teat cups and cooling tanks were associated with raw milk contamination with Pseudomonas spp. on farms with manual and mechanical milking system, showing that regardless of the type of milking system and season, proper hygiene procedures of equipment, utensils and workers’ hands are essential to avoid contamination of the milk and, therefore, improve milk quality.

Apolipoprotein E (APOE=gene, apoE=protein) is a known factor regulating the inflammatory response that may have regenerative effects during tissue recovery from injury. We investigated whether apoE deficiency reduces the healing effect of alanyl-glutamine (Ala-Gln) treatment, a recognized gut-trophic nutrient, during tissue recovery after 5-FU-induced intestinal mucositis. APOE-knockout (APOE-/-) and wild-type (APOE+/+) C57BL6J male and female mice (N=86) were given either Ala-Gln (100 mM) or phosphate buffered saline (PBS) by gavage 3 days before and 5 days after a 5-fluorouracil (5-FU) challenge (450 mg/kg, via intraperitoneal injection). Mouse body weight was monitored daily. The 5-FU cytotoxic effect was evaluated by leukometry. Intestinal villus height, villus/crypt ratio, and villin expression were monitored to assess recovery of the intestinal absorptive surface area. Crypt length, mitotic, apoptotic, and necrotic crypt indexes, and quantitative real-time PCR for insulin-like growth factor-1 (IGF-1) and B-cell lymphoma 2 (Bcl-2) intestinal mRNA transcripts were used to evaluate intestinal epithelial cell turnover. 5-FU challenge caused significant weight loss and leukopenia (P<0.001) in both mouse strains, which was not improved by Ala-Gln. Villus blunting, crypt hyperplasia, and reduced villus/crypt ratio (P<0.05) were found in all 5-FU-challenged mice but not in PBS controls. Ala-Gln improved villus/crypt ratio, crypt length and mitotic index in all challenged mice, compared with PBS controls. Ala-Gln improved villus height only in APOE-/- mice. Crypt cell apoptosis and necrotic scores were increased in all mice challenged by 5-FU, compared with untreated controls. Those scores were significantly lower in Ala-Gln-treated APOE+/+ mice than in controls. Bcl-2 and IGF-1 mRNA transcripts were reduced only in the APOE-/--challenged mice. Altogether our findings suggest APOE-independent Ala-Gln regenerative effects after 5-FU challenge.

Difusores de aumento da potência de turbinas eólicas (DAWTs) têm sido amplamente estudados, uma vez que os difusores melhoraram o coeficiente de potência de turbinas eólicas, particularmente dos sistemas de pequeno porte. O difusor é um dispositivo que tem a função de provocar um aumento da velocidade do vento que passa através do plano do rotor eólico, devido a queda de pressão a jusante do difusor, por conseguinte, resultando em um aumento do coeficiente de potência do rotor. Esta tecnologia faz com que a turbina exceda o limite de Betz, onde a energia cinética máxima extraída do fluxo é de 59,26%. Assim, o presente estudo propõe um modelo matemático que descreva o comportamento do perfil de velocidade interna a três difusores cônicos, considerando as características do escoamento em torno deles. O modelo proposto baseia-se na lei de Biot-Savart, em que o filamento de vórtice induz um campo de velocidade num ponto arbitrário nos eixos de simetria dos difusores. Os resultados são comparados com dados experimentais obtidos para os três difusores, apresentando boa concordância.

The Diffuser Augmented Wind Turbines (DAWTs) have been widely studied, since the diffusers improve the power coefficient of the wind turbine, particularly of small systems. The diffuser is a device which has the function of causing an increase on the flow velocity through the wind rotor plane due to pressure drop downstream, therefore resulting in an increase of the rotor power coefficient. This technology aids the turbine to exceed the Betz limit, which states that the maximum kinetic energy extracted from the flow is 59.26%. Thus, the present study proposes a mathematical model describing the behavior of the internal velocity for three conical diffusers, taking into account the characteristics of flow around them. The proposed model is based on the Biot-Savart's Law, in which the vortex filament induces a velocity field at an arbitrary point on the axis of symmetry of the diffusers. The results are compared with experimental data obtained for the three diffusers, and present good agreement.

Objetivou-se com este trabalho avaliar desempenho, parâmetros de eficiência e correlações fenotípicas entre medidas de eficiência energética de animais Nelore selecionados para peso pós-desmame e classificados quanto ao consumo alimentar residual, calculado pela diferença entre o consumo observado e o predito, com base no peso vivo médio metabólico e no ganho médio diário. Assim, os animais foram classificados em três grupos: alto (> média + 0,5 desvio-padrão; menos eficientes); médio (± 0,5 desvio-padrão da média); e baixo (< média - 0,5 desvio padrão; mais eficientes) consumo alimentar residual. Não foram observadas diferenças nos pesos vivos inicial e final, no ganho médio diário e no consumo de matéria seca entre os grupos. Animais com baixo consumo alimentar residual mostraram-se também com melhor eficiência alimentar, conversão alimentar e eficiência parcial de crescimento e não apresentaram diferenças em relação aos outros grupos quanto à taxa de crescimento relativo e taxa Kleiber. O consumo alimentar residual apresentou correlação significativa com eficiência alimentar (_0,25), conversão alimentar (0,25), eficiência parcial de crescimento (_0,37) e consumo de MS (0,16) e não apresentou correlação significativa com peso vivo (0,04), ganho médio diário (_0,02), taxa de crescimento relativo (_0,03) e taxa de Kleiber (_0,05). Foram encontradas correlações significativas entre conversão alimentar e peso vivo inicial (0,34) e ganho médio diário (_0,46). Eficiência parcial de crescimento apresentou correlação significativa comtodos os outros parâmetros de eficiência analisados. O consumo alimentar residual, em comparação às demais medidas de eficiência energética, apresenta grande potencial na eficiência produtiva, sendo independente de crescimento e tamanho dos animais.

The objective of this work was to evaluate performance, efficiency parameters and phenotypic correlations among measurements of energy efficiency of Nellore cattle selected for post weaning weight and classified according to residual feed intake, calculated by the difference between observed and predicted intake, based on average metabolic body weight and average daily gain. Thus, animals were classified within three groups: high (> mean + 0.5 standard deviation, less efficient); medium (±0.5 standard deviation of the mean); and low (< mean - 0.5 standard deviation, more efficient) residual feed intake. No differences were observed at initial and final body weights, average daily gain and dry matter intake among groups. Animals with low residual feed intake also had greater feed efficiency, feed conversion and partial efficiency of growth and did not differ from the other animals regarding to relative growth rate and Kleiber ratio. Residual feed intake was significantly correlated to feed efficiency (-0.25), feed conversion (0.25), partial efficiency of growth (-0.37) and dry matter intake (0.16) but it did not present significant correlation with body weight (0.04), average daily gain (-0.02), relative growth rate (-0.03) and Kleiber ratio (-0.05). Significant correlations were found between feed conversion and initial body weight (0.34) and average daily gain (-0.46). Partial efficiency of growth presented significant correlation with all other efficiency parameters analyzed. Residual feed intake has high potential in productive efficiency, when compared to the other energy efficiency measurements, being independent of growth and size of the animals.

Realizou-se um estudo para caracterizar a situação epidemiológica da brucelose bovina no Estado do Rio de Janeiro. O Estado foi dividido em três circuitos produtores. Em cada circuito foram amostradas aleatoriamente cerca de 300 propriedades e, dentro dessas, foi escolhido, de forma aleatória, um número pré-estabelecido de animais, dos quais foi obtida uma amostra de sangue. No total foram amostrados 8239 animais, provenientes de 945 propriedades. Em cada propriedade amostrada foi aplicado um questionário epidemiológico para verificar o tipo de exploração e as práticas zootécnicas e sanitárias que poderiam estar associadas ao risco de infecção pela doença. O protocolo de testes utilizado foi o da triagem com o teste do antígeno acidificado tamponado e reteste dos positivos com o teste do 2-mercaptoetanol. O rebanho foi considerado positivo se pelo menos um animal foi reagente às duas provas sorológicas. Para o Estado, as prevalências de focos e de animais infectados foram, respectivamente, de 15,4% [12,9-17,9%] e de 4,1% [2,8-5,3%]. Para os circuitos, as prevalências de focos e de animais infectados foram, respectivamente: circuito 1, 13,8% [10,2-18,2%] e 3,0% [1,9-4,1%]; circuito 2, 15,7% [11,9-20,2%] e 2,3% [1,4-3,2%]; circuito 3, 19,6% [15,4-24,4%] e 9,3% [4,5-14,1%]. Os fatores de risco (odds ratio, OR) associados à condição de foco foram: ter mais que 30 fêmeas com idade de 24 meses ou acima (OR=2,33 [1,51-3,07]), compra de reprodutores (OR= 1,95 [1,13-2,45]) e prática de aluguel de pasto (OR= 1,74 [1,03-2,74]).

A study to characterize the epidemiological status of bovine brucellosis in the State of Rio de Janeiro was carried out. The State was divided in three regions. Three hundred herds were randomly sampled in each region and a pre-established number of animals were sampled in each herd. A total of 8,239 serum samples from 945 herds were collected. In each herd, it was applied an epidemiological questionnaire focused on herd traits as well as husbandry and sanitary practices that could be associated with the risk of infection. The serum samples were screened for antibodies against Brucella spp. by the Rose-Bengal Test (RBT), and all positive sera were re-tested by the 2-mercaptoethanol test (2-ME). The herd was considered positive if at least one animal was positive on both RBT and 2-ME tests. The prevalences of infected herds and animals in the State were, respectively: 15.4% [12.9-17.9%] and 4.1% [2.8-5.3%]. The prevalences of infected herds and animals in the regions were, respectively: region 1, 13.8% [10.2-18.2%] and 3.0% [1.9-4.1%]; region 2, 15.7% [11.9-20.2%] and 2.3% [1.4-3.2%]; and region 3, 19.6% [15.4-24.4%] and 9.3% [4.5-14.1%]. The risk factors (odds ratio, OR) associated with the presence of the infection were: herd size larger than 30 cows (OR=2.33 [1.51-3.07]), purchase of animals for breeding (OR= 1.95 [1.13-2.45), and pasture rental practice (R= 1.74 [1.03-2.74]).

Realizou-se um estudo para caracterizar a situação epidemiológica da brucelose bovina no Distrito Federal (DF). No total foram amostrados 2.019 animais, provenientes de 278 propriedades. Em cada propriedade visitada aplicou-se um questionário epidemiológico para verificar o tipo de exploração e as práticas de criação e sanitárias que poderiam estar associadas ao risco de infecção pela doença. O protocolo utilizado foi o da triagem com o teste do antígeno acidificado tamponado e a confirmação dos positivos com o teste do 2-mercaptoetanol. O rebanho foi considerado positivo quando pelo menos um animal foi reagente às duas provas sorológicas. A prevalência no DF foi de 2,5% [1,0-5,1%] para propriedades e de 0,16% [0,04-0,28%] para animais. Em razão dos resultados encontrados, que permitem pensar em estratégias de erradicação, recomenda-se que o DF intensifique o diagnóstico de brucelose, tanto na forma de testes sorológicos sistemáticos como pela introdução de mecanismos de detecção rápida em laticínios, em ambos os casos a fim de aumentar o número de propriedades certificadas como livres da doença e melhorar a sensibilidade do sistema de vigilância ativa.

A study to characterize the epidemiological status of brucellosis was carried out in the Federal District of Brazil. A total of 2,019 serum samples from 278 herds were collected. In each herd, it was applied an epidemiological questionnaire focused on herd traits as well as husbandry and sanitary practices that could be associated with the risk of infection. The serum samples were screened for antibodies against Brucella spp. by the Rose-Bengal Test (RBT), and all positive sera were re-tested by the 2-mercaptoethanol test (2-ME). The herd was considered positive if at least one animal was positive on both RBT and 2-ME tests. Herd prevalence was estimated as 2.5% [1.0-5.1%] whereas animal prevalence was 0.16% [0.04-0.28%]. Given these results, that may warrant the implementation of eradication strategies, there is a case for intensifying the serological testing in the Federal District, coupled with the introduction of rapid detection methods by the dairy industries, with a view to increasing the number of accredited free herds and improving the sensitivity of the surveillance system..

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is an emergent pathogen in Brazil. However, there are no data on the prevalence of CA-MRSA. We report here the first well-characterized case of severe life-threatening CA-MRSA infection in a child living in Rio de Janeiro city. The patient had many complications including hematogenous osteomyelitis and involvement of multiple sites requiring drainage of soft-tissue abscess, and pleural and pericardial empyema. The MRSA isolates recovered were genotyped using PFGE, SCCmec typing and multilocus sequence typing. Disk diffusion tests were performed following Clinical and Laboratory Standards Institute recommendations. In addition, the presence of Panton-Valentine leukocidin (PVL) was assessed by PCR amplification, using specific primers for lukF-pv (encoding for the F subunit of the PVL). The bacterial isolates were related to the ST30-SCCmecIV lineage (Oceania Southwest Pacific clone), a PVL producer CA-MRSA previously detected in Porto Alegre, RS, Brazil. Also, the isolates analyzed were susceptible to all non-β-lactam antibiotics tested. The present report demonstrates that disseminated CA-MRSA disease is also occurring in Rio de Janeiro. Thus, the empirical treatment of moderate or severe infections suspected of being associated with CA-MRSA needs to be reviewed in order to allow prompt initiation of an effective therapy that also covers these microorganisms.

Data from 1 698 animals belonging to an experimental Caracu herd were analysed with the objective of calculating the population selection differentials, generation intervals, effective size and inbreeding coefficients, and to estimate the direct and correlated responses for growth traits after 23 years of selection for post-weaning weight. The following traits were analysed: birth weight (BW), weaning weight corrected to 210 days of age (W210), weight corrected to 378 days for the males (W378), weight corrected to 550 days for the females (W550) and hip height for the males (HGTm) at the end of a feedlot test, and for the females (HGTf) at 550 days of age. The sire (W378) and dam (W550) weighted selection differentials for post-weaning weight were 1.10 and 0.28 standard deviation units and generation intervals 5.2 and 6.2 years, respectively. Mean inbreeding coefficient increased in the last five years, from 0.49 to 1.95%. The heritability estimates were 0.38, 0.27, 0.36, 0.37, 0.35 and 0.41 for BW, W210, W378, W550, HGTm and HGTf, respectively. The annual genetic trend estimates were 0.98 ± 0.17 kg/year for W378 and 0.27 ± 0.15 kg/year for W550. The estimates for the correlated responses to selection were 0.08 ± 0.02 kg/year for BW, 0.48 ± 0.11 kg/year for W210, 0.06 ± 0.02 cm/year for HGTm and 0.02 ± 0.02 cm/year for HGTf. These results confirmed that direct selection for post-weaning weight based on individual performance was relatively effective in achieving progress in all the growth traits.

Utilizaram-se 86 bovinos, castrados e não-castrados, com peso médio de 329kg e 20 meses de idade, sendo 12 Gir, 20 Guzerá, 20 Nelore e 20 Caracu - todos selecionados para peso aos 378 dias de idade (P378) - e 14 Nelore controle-selecionados com base no diferencial de seleção nulo para P378. A dieta, usada na relação volumoso:concentrado de 60:40 na matéria seca (MS), continha 14,8% de proteína bruta na MS e silagem de milho. O delineamento experimental utilizado foi o inteiramente ao acaso, em esquema fatorial 5 x 2 (grupos genético e classe sexual). Os ganhos médios diários de 1,51kg para Nelore seleção e 1,53kg para Guzerá foram semelhantes e maiores que os dos demais grupos genéticos, os quais não diferiram entre si. Não houve diferenças de ganho entre animais castrados e não-castrados. O consumo de MS em kg/dia foi maior para Nelore, Guzerá e Caracu selecionados (10,30; 10,04 e 10,71kg/dia, respectivamente), e os valores para os demais foram semelhantes entre si. A eficiência bionutricional foi pior para a raça Caracu (2,13) e melhor para Nelore controle (1,58). Os animais Caracu precisaram de maior tempo em confinamento (133 dias) para atingir 4mm de espessura de gordura subcutânea, sendo os valores para as demais raças próximos entre si.

Eighty-six castrated and non-castrated, approximately 20-month-old bovines averaging 329kg were used. They were 12 Gyr, 20 Nellore, 20 Guzerá and 20 Caracu, which were submitted to selection to weight at 378 day-old (W378) and 14 control Nellore - chosen based on nule selection differential to W378. The diet contained 14.8% of crude protein in dry matter (DM) and corn silage was used in the ratio of 60:40% forage: concentrate in DM basis. A completely randomized design in a 5 x 2 factorial arrangement (breed and sexual condition) was used. The average daily gain of 1.51kg and 1.53kg for selected Nellore and Guzerá did not differ, however surpassed those from the other breeds, which did not differ from each other. There was no difference between castrated and non-castrated animals. The dry matter intake in kg/day were higher for selected Nellore, Guzerá and Caracu (10.30; 10.04 and 10.71kg/day, respectively) and the values observed for the two other groups did not differ from each other. The bionutritional efficiency was worse for Caracu (2.13) and better for control Nellore (1.58). Caracu needed to remain in feeding 133 days longer to reach 4mm of subcutaneous fat thickness. The time in feeding for the others groups were similar.

Foram avaliados cinco grupos genéticos quanto à eficiência bionutricional (índice resultante da primeira função canônica de uma análise bivariada que envolveu ganho de peso e consumo de matéria seca) sendo seis animais da raça Nelore (N), cinco 1/2Romosinuano-1/2Caracu (RSC), quatro 1/2Senepol-1/2Caracu (SEC), quatro 1/2Belmont Red-1/2Caracu (BRC) e sete 1/2Valdostana-1/2Nelore (VAN). Não foi observada diferença significativa no desempenho dos animais mestiços Caracu, cuja média de eficiência bionutricional foi 519,8. O Nelore apresentou a menor eficiência bionutricional, 425,07, e os animais F1 Valdostana-Nelore, 499,30. Tendência semelhante foi observada para o ganho de peso. Os mestiços que envolveram raças européias adaptadas (RSC, SEC, BRC) apresentaram média de ganho de peso de 1,69kg/dia, enquanto os F1s Valdostana-Nelore alcançaram 1,57kg/dia e os da raça Nelore 1,29kg/dia. Não houve diferença no consumo diário de matéria seca entre os grupos genéticos, média de 7,76kg de MS ingerida/dia. Os grupos genéticos apresentaram conversão alimentar semelhante, com média de 4,87kg de MS ingerida/kg de ganho de peso.

Five genetic groups were evaluated by bio-nutritional efficiency (index obtained from the first canonical function in a bivariate analysis encompassing weight gain and dry matter intake): six Nellore (N), five 1/2Romosinuano-1/2Caracu (RSC), four 1/2Senepol-1/2Caracu (SEC), four 1/2Belmont Red-1/2 Caracu (BRC) and seven 1/2Valdostana-1/2Nellore (VAN). No differences were observed among the Caracu crosses for bio-nutritional efficiency, which averaged 519.8. Nellore showed the smallest performance (425.07), while the Vasdostana-Nellore F1's averaged 499.30. Similar trend was observed for daily weight gain. The crossbred animals involving European adapted breeds (RSC, SEC, BRC) showed an average gain of 1.69kg/day, while the F1's Valdostana-Nelore and the Nellore gains were, respectively, 1.57kg/day and 1.29kg/day. No difference for dry matter (DM) intake among genetic groups which averaged 7.76kg DM intake/day was observed. As far as feed conversion is concerned, no difference among genetic groups, which averaged 4.87kg of DM intake/kg of weight gain, was observed.