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au:FONSECA, MARIA J. M.

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Potential Application of Membrane Obtained from Sisal Bagasse in the Removal of Inhibitors from the Fermentation Process
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Xavier, Franklin D.

; Silva, Aleir J. O.

; Lima, Marcos S.

; Fonseca, Maria G. da

; Conceição, Marta M. da

Journal of the Brazilian Chemical Society

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oct 2023, Volume 34 N. 10 Pages 1531 - 1540

Abstract: En | Text: En | PDF: En

Annual world production of sisal is estimated to be 400,000 tons, but the processing of sisal fiber generates around 160,000 tons of waste per year. The sisal bagasse is discarded, even though it is about 60% carbohydrates in its structure. Therefore, this work seeks to add value to sisal bagasse through the production of cellulose acetate membranes aimed at removing inhibitors from the fermentation process. The membrane was developed using a cellulose acetate polymer synthesized from cellulose isolated from sisal bagasse. Alkaline and acid pre-treatments resulted in 90% pure cellulose. The obtained cellulose acetate was confirmed by infrared spectroscopy. In the synthesis of the membranes, the influence of agitation time and temperature of the bath on the selectivity and porosity were evaluated in four tests, the third condition stood out with porosity of 26.23% and selectivity of 94.51% in the removal of the furfural and 36.62% of the acetic acid.

https://doi.org/10.21577/0103-5053.20230066

COVID-19 Infection Changes the Functions and Morphology of Erythrocytes: A Multidisciplinary Study
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Sales, Marcos V. S.

; Tanabe, Eloiza L. L.

; Maciel, Thamilla M. S.

; Tavares, Maria C.

; Leal, Juliana G. C.

; Pinto, Larissa S.

; Pires, Keyla S. N.

; Coelho, Jorge A. P. M.

; Silva, Elaine C. O.

; Souza, Samuel T.

; Fonseca, Eduardo J. S.

; Fragoso, Thiago S.

; Aquino, Thiago M.

; Borbely, Alexandre U.

; Rocha, Ueslen

; Santos, Josué C. C.

; Leite, Ana Catarina R.

Journal of the Brazilian Chemical Society

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aug 2023, Volume 34 N. 8 Pages 1185 - 1196

Abstract: En | Text: En | PDF: En

Hematological problems are associated with Coronavirus disease 2019 (COVID-19). Respiratory impairment is the higher point studied, although without experimental studies related to the oxygen transport performed by erythrocytes. Therefore, we decided to investigate if erythrocytes from COVID-19 patients have their functionality changed. The case-control study included hospitalized patients with a positive real-time polymerase chain reaction (RT-PCR) result admitted to University Hospital. Volunteers (negative RT-PCR results) were recruited as a control group. Thus, we assessed different erythrocytes parameters, oxidative stress markers, and biophysical studies using whole blood and isolated hemoglobin. We found a decrease of 51% in oxygen uptake and reduced antioxidant enzyme activity in COVID-19 patients compared to controls. Raman spectrometry showed structural changes in the hemoglobin and lipids of the erythrocytes from COVID-19 patients; thus, these results were consolidated with an increase in Young’s modulus in erythrocytes followed by morphology changes. Besides, isolated hemoglobin from COVID-19 patients has a distinct interaction profile using a ligand model compared to the control. COVID-19 leads to structural, functional, and morphological damage to human erythrocytes. Our data showed structural and molecular changes and induction of oxidative stress in erythrocytes by COVID-19, a new perspective on the contribution of erythrocytes to a respiratory commitment in COVID-19.

https://doi.org/10.21577/0103-5053.20230031

Deep sea ecosystem exploration and ‘health check’: sampling strategy and methods applied during the iAtlantic_BR10_Petrobras cruise in the Santos Basin, Southwest Atlantic
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Perez, Jose Angel A.

; Barros Neto, Halésio M.C. de

; Arantes, Renata C.M.

; Gaurisas, Daniela Y.

; Silva, Camila F.

; Alves, Fernanda Maria M.

; Costa, Júlia A.

; Eloi, Paula D.C.

; Fonseca, Thayse S.

; Gavazzoni, Lucas

; Lonskis, Isabella da S.

; Nardi, Ricardo U.

; Nascimento, Pedro O.

; Rodrigues, João Vitor M.

; Santos, Ana Lara F. dos

; Santos, Edson C. dos

; Schroeder, Rafael

; Silva, Luis Carlos da

; Souza, Fernanda dos S.S. de

; Bernardino, Ângelo F.

; Cavalcanti, Guarani H.

; Lindner, Alberto

; Mahiques, Michel M. de

; Millo, Christian

; Reis, Priscila

; Sweetman, Andrew K.

; Roberts, J. Murray

Ocean and Coastal Research

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2023, Volume 71 elocation e23046

Abstract: En | Text: En | PDF: En

Abstract The iAtlantic Project has established an international collaborative strategy to improve mapping and characterization of deep and open ocean ecosystems in understudied regions of the Atlantic and evaluate their health. In December 2022, the first iAtlantic expedition in the South Atlantic set off to map and explore seafloor ecosystems in the Santos Basin slope (200-1,000 m depths) in collaboration with the Petrobras ‘Santos Basin - Regional Characterization Project.’ The 17-day ‘iAtlantic_BR10-Petrobras’ cruise was conducted on board the research vessel NPqHOc Vital de Oliveira (Brazilian Navy) and performed (a) water column structure characterization, (b) seafloor morphology mapping, (c) description of benthic habitats and communities by seafloor imagery and biological/ geological sampling, and (d) ex-situ experiments to assess the functioning of sedimentary ecosystems and their responses to climate-related environmental changes. This study describes the rationale behind the iAtlantic_BR10-Petrobras cruise science plan, reports its sampling strategy and methods, and summarizes its collected data and preliminary results.

https://doi.org/10.1590/2675-2824071.23069jaap

Fighting HIV/AIDS in a developing country: lessons from a small cohort from the largest Brazilian city
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Casseb, Jorge

; Veiga, Ana Paula R.

; Magri, Marcello M. C.

; Monteiro, Mariana A.

; Rocha, Rosana C.

; Gascon, Maria Rita P.

; Domingues-Ferreira, Mauricio

; Polis, Thales J. B.

; Nascimento, Najara A. de Lima

; Limongelli, Isadora

; Oliveira, Ícaro S.

; Prandi, Gabriela Caetano

; Costa, Livia M. C. B. Villares

; Fonseca, Luiz A. M.

; Duarte, Alberto J. S.

Revista do Instituto de Medicina Tropical de São Paulo

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2020, Volume 62 elocation e58

Abstract: En | Text: En | PDF: En

https://doi.org/10.1590/s1678-9946202062058 221 downloads

A New Cytotoxic Naphthoquinone and Other Chemical Constituents of Sinningia reitzii
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Silva, Adson S.

; Amorim, Magali S.

; Fonseca, Mariana M.

; Salvador, Marcos J.

; Sá, Eduardo L. de

; Stefanello, Maria Élida A.

Journal of the Brazilian Chemical Society

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oct 2019, Volume 30 N. 10 Pages 2060 - 2065

Abstract: En | Text: En | PDF: En

Phytochemical study of Sinningia reitzii (Gesneriaceae) led to the isolation of three new naphtoquinones, 6,7-dimethoxydunnione, 7-hydroxy-6-methoxydunnione, and 5-hydroxy-6,7-dimethoxydunnione from the tubers, together with four known compounds, 7-hydroxydunnione, 8-hydroxydunnione, 5-hydroxy-6,7-dimethoxy-α-dunnione, and 8-hydroxydeydrodunnione. Aerial parts furnished five known compounds, 5-hydroxy-6,7-dimethoxydunniol, 6,8-dihydroxy-7-methoxy-2-O-methyldunniol, loureirin B, jacaranone, and methyl 4-hydroxyphenylacetate. Compounds 8-hydroxydunnione, 5-hydroxy-6,7-dimethoxy-α-dunnione, 8-hydroxydeydrodunnione, 5-hydroxy-6,7-dimethoxydunniol and 6,8-dihydroxy-7-methoxy-2-O-methyldunniol had been previously reported in the tubers of S. reitzii. Density functional theory was used to assign the absolute configuration of compounds 6,7-dimethoxydunnione, 7-hydroxy-6-methoxydunnione 5-hydroxy-6,7-dimethoxydunnione, 7-hydroxydunnione, 8-hydroxydunnione, 5-hydroxy-6,7-dimethoxy-α-dunnione. Compounds 6,7-dimethoxydunnione, 8-hydroxydunnione, 5-hydroxy-6,7-dimethoxy-α-dunnione and 8-hydroxydeydrodunnione were evaluated for cytotoxicity against PC3 (prostate), SKMEL 103 (melanoma), and HeLa (cervix) human cancer, and 3T3 fibroblast cell lines, using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Compound 6,7-dimethoxydunnione displays cytotoxic activity against all the tumor cell lines tested (half maximal inhibitory concentration, IC50, 4.47-26.2 µmol L-1), and was inactive against 3T3 fibroblasts (IC50 > 100 µmol L-1). Compounds 8-hydroxydunnione, 5-hydroxy-6,7-dimethoxy-α-dunnione and 8-hydroxydeydrodunnione were inactive against all tested cell lines.

https://doi.org/10.21577/0103-5053.20190054 281 downloads

First Colombian consensus on congenital Chagas and clinical approach for women of child-bearing age diagnosed with Chagas
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Cucunubá, Zulma M.

; Valencia-Hernández, Carlos A.

; Puerta, Concepción J.

; Sosa-Estani, Sergio

; Torrico, Faustino

; Cortés, Jorge Alberto

; Ramirez, Juan David

; Vera, Mauricio J.

; Acosta, Belkis Xiomara

; Álvarez, Carlos Arturo

; Muller, Edith Ángel

; Beltrán, Mauricio

; Bermúdez, María Isabel

; Berrío, Maritza

; Camacho Moreno, Germán

; Castellanos, Yeny Zulay

; Criollo, Ingrid

; Flórez, Astrid Carolina

; Guerra Morales, Patricia

; Herazo, Rafael Antonio

; Hernández, Diana Carolina

; León, Cielo Maritza

; Medina Camargo, Manuel

; MedinaAlfonso, Mabel

; Pachón, Edwin

; Paez Fonseca, Bernardo

; Parra, María Luisa

; Pavia, Paula X.

; Quiróz, Franklin Roberto

; Ríos, Lyda Constanza

; Roa, Nubia Lucía

; Torres, Fernando

; Uribe Rivero, Luz Marina

Infectio

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dec 2017, Volume 21 N. 4 Pages 255 - 266

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen La transmisión congénita de la enfermedad de Chagas ha sido poco estudiada en Colombia y existen pocos procedimientos rutinarios en el sistema de salud para el manejo de esta enfermedad. Por ello se desarrolló un consenso de expertos dirigido a generar recomendaciones de diagnóstico y tratamiento de Chagas con- génito y orientación a mujeres en edad fértil. Con ese propósito se realizó una búsqueda extensiva de la literatura, empleando una combinación de términos Mes (Chagas, Chagas congénito, prevención, control, diagnóstico, tratamiento y embarazo) para reflejar el estado del arte en cada tema de interés. Después de ello, se leyeron los resúmenes y aquellos seleccionados para análisis del texto completo. La literatura relevante se sintetizo, clasifico y organizo en tablas y se presentó al panel de expertos, el cual estaba constituido por 30 profesionales en diferentes áreas. Mediante la metodología Delphi se realizaron 2 rondas de cuestionarios virtuales y una reunión presencial en los cuales se evaluaron los niveles de acuerdo entre los participantes. Los puntos con falta de consenso durante las 2 rondas virtuales se expusieron durante las mesas de discusión en la ronda presencial. La evidencia utilizada se adaptó a las particularidades nacionales según el caso y se aprobó el contenido del documento final. Se propone que estas recomendaciones sean usadas por profesionales de la salud en Colombia.

Abstract Congenital transmission of Chagas disease has not been extensively studied in Colombia, and there are no standardized processes in the health system regarding the specific diagnosis, treatment and follow-up of this disease. To generate recommendations on congenital Chagas disease and Chagas in women of childbearing age in Colombia, a consensus of experts was developed. An extensive literature search through the Medline database was carried out using the MeSH terms: «Chagas disease/congenital», «prevention and control», «diagnosis», «therapeutics» and «pregnancy». Appropriate abstracts were selected and the full texts were analyzed. The relevant information was synthesized, classified, and organized into tables and figures and was presented to a panel of experts, which was composed of 30 professionals from various fields. Based on the Delphi methodology, three rounds of consultation were conducted. The first and second rounds were based on electronic questionnaires that measured the level of consensus of each question among the participants. The third round was based on a face-to-face discussion focusing on those questions without consensus in the previous consultations. The evidence was adapted to national circumstances on a case-by-case basis, and the content the final document was approved. These recommendations are proposed for use in routine medical practice by health professionals in Colombia.

https://doi.org/10.22354/in.v21i4.689 799 downloads

Structure and histochemistry of medicinal species of Solanum
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Matias, Laudineia J.

; Mercadante-Simões, Maria O.

; Royo, Vanessa A.

; Ribeiro, Leonardo M.

; Santos, Ariadna C.

; Fonseca, Jaciara M.S.

Revista Brasileira de Farmacognosia

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apr 2016, Volume 26 N. 2 Pages 147 - 160

Abstract: En | Text: En | PDF: En

ABSTRACT Studies on native medicinal plants strengthen initiatives to preserve the environments where those species naturally occur, many of them already strongly menaced even before their potential to humankind is known. Root and stem barks, leaves, and pericarps samples of Solanum agrarium Sendtn., S. lycocarpum A. St.-Hil., S. palinacanthum Dunal, S. paniculatum L., and S. stipulaceum Roem. & Schult., species that occur in the Cerrado (Brazililan savanna) were processed according to common light microscopy techniques for structural analysis, and histochemical tests were performed to locate and identify classes of chemical compounds. The distinctive features identified were low concentration of crystal sand in the root and stem, presence of terpene resin in the root, and absence of hypodermis in the leaf, in S. agrarium; bright spots (group of sclereids) in the root, isobilateral mesophyll, thickened cell walls with hemicelluloses and strong aroma in the fruit, in S. lycocarpum; high concentration of crystal sand in the root and stem, oval-shaped limb, presence of isolated crystals in the exocarp, in S. palinacanthum; strong sclerification and rays with great height in the root and stem, in S. paniculatum; and accumulation of soluble protein in the root and stem, presence of conspicuous membranaceous stipules, absence of spiniform trichomes, in S. stipulaceum. This work identifies distinctive structural features, its ecological importance, and determines the distribution of secondary compounds associated with the medicinal properties reported for these species and contributes to the conservation of the natural environments where they occur.

https://doi.org/10.1016/j.bjp.2015.11.002 2605 downloads

Primer consenso colombiano sobre Chagas congénito y orientación clínica a mujeres en edad fértil con diagnóstico de Chagas
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Cucunubá, Zulma M.

; Valencia-Hernández, Carlos A.

; Puerta, Concepción J.

; Sosa-Estani, Sergio

; Torrico, Faustino

; Cortés, Jorge Alberto

; Ramirez, Juan David

; Vera, Mauricio J.

; Acosta, Belkis Xiomara

; Álvarez, Carlos Arturo

; Muller, Edith Ángel

; Beltrán, Mauricio

; Bermúdez, María Isabel

; Berrío, Maritza

; Camacho Moreno, Germán

; Castellanos, Yeny Zulay

; Criollo, Ingrid

; Flórez, Astrid Carolina

; Guerra Morales, Patricia

; Herazo, Rafael Antonio

; Hernández, Diana Carolina

; León, Cielo Maritza

; Medina Camargo, Manuel

; Medina Alfonso, Mabel

; Pachón, Edwin

; Paez Fonseca, Bernardo

; Parra, María Luisa

; Pavia, Paula X.

; Quiróz, Franklin Roberto

; Ríos, Lyda Constanza

; Roa, Nubia Lucía

; Torres, Fernando

; Uribe Rivero, Luz Marina

Infectio

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jun 2014, Volume 18 N. 2 Pages 50 - 65

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

La transmisión congénita de la enfermedad de Chagas ha sido poco estudiada en Colombia y existen pocos procedimientos rutinarios en el sistema de salud para el manejo de esta enfermedad. Por ello se desarrolló un consenso de expertos dirigido a generar recomendaciones de diagnóstico y tratamiento de Chagas congénito y orientación a mujeres en edad fértil. Con ese propósito se realizó una búsqueda extensiva de la literatura, empleando una combinación de términos MeSH (Chagas, Chagas congénito, prevención, control, diagnóstico, tratamiento y embarazo) para reflejar el estado del arte en cada tema de interés. Después de ello, se leyeron los resúmenes y aquellos seleccionados para análisis del texto completo. La literatura relevante se sintetizó, clasificó y organizó en tablas y se presentó al panel de expertos, el cual estaba constituido por 30 profesionales en diferentes áreas. Mediante la metodología Delphi se realizaron 2 rondas de cuestionarios virtuales y una reunión presencial en los cuales se evaluaron los niveles de acuerdo entre los participantes. Los puntos con falta de consenso durante las 2 rondas virtuales se expusieron durante las mesas de discusión en la ronda presencial. La evidencia utilizada se adaptó a las particularidades nacionales según el caso y se aprobó el contenido del documento final. Se propone que estas recomendaciones sean usadas por profesionales de la salud en Colombia.

Congenital transmission of Chagas disease has not been extensively studied in Colombia, and there are no standardized processes in the health system regarding the specific diagnosis, treatment and follow-up of this disease. In order to generate recommendations on congenital Chagas disease and Chagas in women of childbearing age in Colombia, a consensus of experts was developed. An extensive literature search through the Medline database was carried out using the MeSH terms: " Chagas disease/congenital " , " prevention and control " , " diagnosis " , " therapeutics " and " pregnancy " . Appropriate abstracts were selected and the full texts were analyzed. The relevant information was synthesized, classified, and organized into tables and figures and was presented to a panel of experts, which was composed of 30 professionals from various fields. Based on the Delphi methodology, three rounds of consultation were conducted. The first and second rounds were based on electronic questionnaires that measured the level of consensus of each question among the participants. The third round was based on a face-toface discussion focusing on those questions without consensus in the previous consultations. The evidence was adapted to national circumstances on a case-by-case basis, and the content the final document was approved. These recommendations are proposed for use in routine medical practice by health professionals in Colombia.

https://doi.org/10.1016/j.infect.2013.12.001 2694 downloads

Composição corporal e desempenho motor em escolares da rede pública de ensino
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Albuquerque-Filho, Nailton J. Brandão de

; Felipe, Thiago R.

; Mendes-Rebouças, Gleidson

; Conceição-Maia, Ubilina M.

; Fonseca-Pinto, Edson

; Costa, Adalberto Veronese da

; Knackfuss, Maria I.

; Medeiros, Humberto J. De

Revista de Salud Pública

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dec 2013, Volume 15 N. 6 Pages 834 - 843

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Objetivo Analisar as influências da composição corporal sobre o desempenho motor de escolares da rede pública de ensino. Métodos Trata-se de um estudo de corte transversal desenvolvido com uma amostra 371 participantes do sexo feminino na faixa etária de sete a 16 anos matriculadas na rede pública de ensino e foram divididos em três grupos: Grupo 1 (G1), Grupo 2 (G2) e Grupo 3 (G3). Foram coletados peso e estatura para avaliar o índice de massa corporal (IMC), as dobras cutâneas de tríceps e subescapular para avaliar o percentual de gordura (% G), além dos testes de sentar e alcançar e o de velocidade de deslocamento de 20 metros. Resultados Foram encontrados diferenças significativas em todas as variáveis antropométricas. Entre as 371 participantes 41,4 % das escolares encontraram-se com sobrepeso ou obesidade, onde 66,1 % no G1, 34,5 % no G2 e 25,3 % no G3. Com relação ao % G, 29,1 % estão acima dos níveis adequados, sendo para G1 23,3 %, para G2 23,8% e para G3 35,2 %. Também nos testes motores foram encontradas diferenças significativas no teste de sentar e alcançar entre G1 e G2 e entre G2 e G3, bem como no teste de velocidade entre G1 e G3. Conclusão A adiposidade pode ter interferido no desempenho motor das escolares, em especial para o teste de velocidade de deslocamento de 20 metros.

Objective Analysing the influence of body composition on female public school students' motor performance. Methods This was a cross-sectional study which involved a sample 371 female public school system students aged seven to 16 years old; they were placed ingroup 1 (G1), group 2 (G2) orgroup 3 (G3). Weight and height were measured for evaluating the body mass index (BMI), triceps and subscapular skinfold thickness for assessing the percentage of body fat (%BF).Sit and reach and 20 meters displacement speed tests were also used. Results Significant differences were found regarding all anthropometric variables considered. It was found that 41.1% of the 371 female students were overweight or obese (66.1 % in G1, 34.5% in G2 and 25.3 % in G3). Regarding % BF, 29.1% of the students had percentages above suitable levels (23.3 % in G1, 23.8 % in G2 and 35.2 % in G3). Significant differences between G1 and G2 and between G2 and G3 were also found in the motor tests regarding the sit and reach test, as well as between G1 and G3 in the speed test. Conclusion Adiposity may have interfered with the students' motor performance, especially regarding the 20 meters' displacement speed test.

Objetivo Evaluar la influencia de la composición corporal en el desempeño motor de chicas en el sistema escolar público. Método Se trata de un estudio de corte transversal que se llevó a cabo con una muestra de 371 chicas de edades comprendidas entre siete y 16 años, matriculados en el sistema escolar público y fueron divididas en tres grupos: Grupo 1 (G1), Grupo 2 (G2) y Grupo 3 (G3). Se midieron peso y talla para evaluar el índice de masa corporal (IMC), los pliegues cutáneos del tríceps y subescapular para evaluar el porcentaje de grasa corporal (%G), más allá de los testes de sentarse y llegar y velocidad de desplazamiento de 20 metros. Resultados Se encontraron diferencias significativas en todas las variables antropométricas. Entre las 371 participantes, 41,4 % de las chicas tenían sobrepeso u obesidad, donde el 66,1 % para G1, 34,5 % en el G2 y 25,3 % en el G3. Cuanto a la % G, 29,1 % están por encima de los niveles adecuados, con 23.3 % para G1, 23.8 % para G2 y 35.2 % para G3. También en los testes motrices se encontraron diferencias significativas en el teste de sentarse y llegar entre G1 y G2 y entre G2 y G3, así como la prueba de velocidad entre G1 y G3. Conclusiones La adiposidad puede haber interferido en el desempeño motor de las chicas, sobre todo para el teste de velocidad de desplazamiento de 20 metros.

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Composição corporal e desempenho motor em escolares da rede pública de ensino
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Albuquerque-Filho, Nailton J. Brandão de

; Felipe, Thiago R.

; Mendes-Rebouças, Gleidson

; Conceição-Maia, Ubilina M.

; Fonseca-Pinto, Edson

; Costa, Adalberto Veronese da

; Knackfuss, Maria I.

; De Medeiros, Humberto J.

Revista de Salud Pública

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nov 2013, Volume 15 N. 6 Pages 834 - 843

Abstract: Es En Pt | Text: Es En Pt | PDF: Es | PDF: En | PDF: Pt

1541 downloads

11.

Tephrosia sinapou extract reduces inflammatory leukocyte recruitment in mice: effect on oxidative stress, nitric oxide and cytokine production
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Martinez, Renata M.

; Zarpelon, Ana C.

; Zimermann, Vanessa V. M.

; Georgetti, Sandra R.

; Baracat, Marcela M.

; Fonseca, Maria J. V.

; Vicentini, Fabiana T. M. C.

; Moreira, Isabel C.

; Andrei, Cesar C.

; Verri Jr, Waldiceu A.

; Casagrande, Rubia

Revista Brasileira de Farmacognosia

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jun 2012, Volume 22 N. 3 Pages 587 - 597

Abstract: En | Text: En | PDF: En

Tephrosia toxicaria (Sw.) Pers., which is currently known as T. sinapou (Buc'hoz) A. Chev., Fabaceae, is a source of compounds such as flavonoids, however, few studies addressed the anti-inflammatory and antioxidant effects of T. sinapou. Therefore, we evaluated the antioxidant mechanisms of the T. sinapou ethyl acetate extract in vitro, and whether the extract affects leukocyte recruitment in four models of inflammation and the involvement of nitric oxide and cytokines in its mechanism. In vitro, it was observed that the extract presented hydrogen donating ability to 2,2-diphenyl-1-picryl-hydrazyl radical (DPPH<img src="/img/revistas/rbfar/2012nahead/aop23011img01.jpg" />), 2,2'-azino-di-(3-ethylbenzthiazoline-6-sulphonic acid) radical (ABTS+), and also efficiently inhibited iron-dependent and independent lipid peroxidation and iron chelation assays. In vivo, it inhibited the recruitment of total leukocytes and neutrophil induced by carrageenin, zymosan, glycogen and lipopolysaccharide in the peritoneal cavity of mice. Two mechanisms were detected: 1) T. sinapou effect on leukocyte recruitment depends on nitric oxide since was dose-dependently inhibited by treatment with L-NAME (nitric oxide synthase inhibitor), and 2) the extract also inhibited the production of crucial cytokines for the leukocyte recruitment; tumor necrosis factor α and interleukin-1β. Concluding, T. sinapou ethyl acetate extract reduces oxidative stress in vitro, and inflammatory leukocyte recruitment by a mechanism related to inhibition of cytokine production, and in a nitric oxide dependent manner in vivo.

2886 downloads

12.

Consenso brasileiro do tratamento da fibromialgia
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Heymann, Roberto Ezequiel

; Paiva, Eduardo dos Santos

; Helfenstein Junior, Milton

; Pollak, Daniel Feldman

; Martinez, José Eduardo

; Provenza, José Roberto

; Paula, Ana Patrícia

; Althoff, Antonio Carlos

; Souza, Eduardo José do R. e

; Neubarth, Fernando

; Lage, Lais Verderame

; Rezende, Marcelo Cruz

; Assis, Marcos Renato de

; Lopes, Maria Lucia Lemos

; Jennings, Fabio

; Araújo, Rejane Leal C. da Costa

; Cristo, Valéria Valim

; Costa, Evelin Diana Goldenberg

; Kaziyama, Helena Hideko S.

; Yeng, Lin Tchia

; Iamamura, Marta

; Saron, Thais Rodrigues Pato

; Nascimento, Osvaldo J. M.

; Kimura, Luiz Koiti

; Leite, Vilnei Mattioli

; Oliveira, Juliano

; Araújo, Gabriela Tannus Branco de

; Fonseca, Marcelo Cunio Machado

Revista Brasileira de Reumatologia

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feb 2010, Volume 50 N. 1 Pages 56 - 66

Abstract: Pt | Text: Pt | PDF: Pt

92318 downloads Cited 22 times in SciELO

13.

Acurácia de informações sobre classes de medicamentos obtidas com questionário postal aplicado a idosos - Rio de Janeiro, RJ
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Vasconcelos, Maria Estrella L.

; Fonseca, Maria J. M.

; Rozenfeld, Suely

; Acurcio, Francisco A.

Revista Brasileira de Epidemiologia

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dec 2009, Volume 12 N. 4 Pages 578 - 590

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVOS: verificar a confiabilidade e a validade das informações sobre medicamentos obtidas em questionário postal, respondido por idosos, sendo a entrevista face a face o padrão-ouro. MÉTODOS: estudo seccional (Perfil de Utilização de Medicamentos por Aposentados Brasileiros), onde foram utilizadas duas abordagens (postal e domiciliar) para coleta de informações de aposentados pelo Instituto Nacional do Seguro Social (INSS) com sessenta anos de idade ou mais. Foram utilizadas também as estatísticas kappa (simples (k), ajustado (PABAK) e ponderado), índices de correlação intra-classe, indicadores de sensibilidade e especificidade, e o gráfico de Luiz et al. RESULTADOS: 234 idosos (M = 42%; F = 58%) responderam às duas abordagens (média = 71,7 anos). A concordância entre postal e entrevista domiciliar foi excelente (k = 0,94) para hipoglicemiantes; muito boa (k = 0,83-0,82) para inibidores da enzima conversora de angiotensina e anti-hipertensivos; boa (k = 0,71) para diuréticos; e razoável (k = 0,47) para antiinflamatórios não esteróides. A concordância foi boa (k = 0,61) para o número total de medicamentos usados. A validade da abordagem postal foi elevada, às vezes total, para os fármacos empregados no tratamento do diabetes (sensibilidade e especificidade = 100%), seguidos dos anti-hipertensivos. Os menores valores obtidos foram para antiinflamatórios não esteróides (sensibilidade = 64%; especificidade = 88%). CONCLUSÃO: a abordagem postal pode ser usada para se obter informações acuradas sobre classes de medicamentos usados por população com idade igual ou superior a 60 anos, considerando idosos com perfil social semelhante ao dos beneficiários do INSS.

OBJECTIVES: to determine the reproducibility and validity of information on medication obtained in a postal questionnaire, with face-to-face interviews providing the gold standard. METHODS: cross-sectional study (Perfil de Utilização de Medicamentos por Aposentados Brasileiros), using two approaches (postal and home visit) to collect information from retirees who were 60 years old or older and beneficiaries of Brazil's national social security system (INSS). Statistics used were: simple kappa (k), adjusted kappa (PABAK), weighted kappa, intraclass correlation coefficients, indicators of sensitivity and specificity, and Luiz et al's graph method. RESULTS: 234 elderly (male = 42%; female = 58%; mean = 71.7 years) answered both approaches. Concordance between the postal and face-to-face interview approaches was excellent (k=0.94) for hypoglycemics; very good (k = 0.83-0.82) for angiotensin-converting enzyme inhibitors and anti-hypertensives; good (k = 0.71) for diuretics; and reasonable (k = 0.47) for non-steroidal anti-inflammatories. Concordance was good (k = 0.61) for information on the total number of medications used. The validity of the postal approach was high, at times total, for the drugs used in treating diabetes (sensitivity and specificity = 100%), followed by anti-hypertensives. The lowest values obtained were for non-steroidal anti-inflammatories (sensitivity = 64%; specificity = 88%). CONCLUSION: the postal approach can be used to obtain accurate information on classes of medications used by a population aged 60 years or more with a social profile similar to that of social security beneficiaries.

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14.

Acurácia de informações sobre classes de medicamentos obtidas com questionário postal aplicado a idosos - Rio de Janeiro, RJ
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Vasconcelos, Maria Estrella L.

; Fonseca, Maria J. M.

; Rozenfeld, Suely

; Acurcio, Francisco A.

Revista Brasileira de Epidemiologia

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dec 2009, Volume 12 N. 4 Pages 578 - 590

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

2113 downloads

15.

Method validation and stability study of quercetin in topical emulsions
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Casagrande, Rúbia

; Baracat, Marcela M.

; Georgetti, Sandra R.

; Verri Jr, Waldiceu A.

; Vicentini, Fabiana T. M. C.

; Rafael, Janice A.

; Jabor, José R.

; Fonseca, Maria J. V.

Química Nova

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2009, Volume 32 N. 7 Pages 1939 - 1942

Abstract: En | Text: En | PDF: En

This study validated a high performance liquid chromatography (HPLC) method for the quantitative evaluation of quercetin in topical emulsions. The method was linear within 0.05 - 200 μg/mL range with a correlation coefficient of 0.9997, and without interference in the quercetin peak. The detection and quantitation limits were 18 and 29 ng/mL, respectively. The intra- and inter-assay precisions presented R.S.D. values lower than 2%. An average of 93% and 94% of quercetin was recovered for non-ionic and anionic emulsions, respectively. The raw material and anionic emulsion, but not non-ionic emulsion, were stable in all storage conditions for one year. The method reported is a fast and reliable HPLC technique useful for quercetin determination in topical emulsions.

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