Abstract: Creation, adjustments and adoption of tests and tools that help in the prediction of seed storability have been highly demanded. Therefore, this work aimed to analyze the efficiency of different artificial aging times in predicting the performance of soybean seeds after storage, using the GGE biplot method. Seeds of six genotypes were subjected to storage, under refrigerated and non-refrigerated conditions, and artificial aging, being artificially aged for periods of 0, 48, 96 and 144 hours. Seeds freshly harvested and after natural and artificial aging were subjected to germination and vigor tests. The experiments were analyzed separately, using means test, regression analysis and model identity test, and together, using the GGE biplot method. Artificial aging at a temperature of 41 °C for 96 hours has the potential to be used to predict the performance of soybean seeds after eight months of storage. The GGE biplot is a method that can be used as a tool to analyze the relationships between aging environments and visualize the ranking of genotypes regarding the performance of seeds subjected to natural and artificial aging.

Resumo: Tem sido altamente requerida a elaboração, ajustes e adoção de testes e ferramentas que auxiliem na predição da armazenabilidade de sementes. Diante disso, este trabalho teve por objetivo analisar a eficiência de diferentes tempos de envelhecimento artificial na predição do desempenho das sementes de soja após o armazenamento, utilizando-se o método GGE biplot. Sementes de seis genótipos foram submetidas ao armazenamento, sob condição refrigerada e não refrigerada, e envelhecimento artificial, sendo envelhecidas artificialmente pelos períodos de 0, 48, 96 e 144 horas. As sementes recém-colhidas e após o envelhecimento natural e artificial foram submetidas a testes de germinação e vigor. Os experimentos foram analisados separadamente, por meio de teste de médias, análise de regressão e teste de identidade de modelos, e em conjunto, utilizando-se o método GGE biplot. O envelhecimento artificial, à temperatura de 41 °C por 96 horas apresenta potencial para ser utilizado na predição do desempenho de sementes de soja após oito meses de armazenamento. O GGE biplot é um método que pode ser utilizado como ferramenta para analisar as relações entre os ambientes de envelhecimento e visualizar o ranqueamento dos genótipos quanto ao desempenho das sementes submetidas ao envelhecimento natural e artificial.

ABSTRACT BACKGROUND: The effect of weight loss (WL) on histopathological aspects of non-alcoholic fatty liver disease (NAFLD) may provide further insights into the dynamics of hepatic recovery after WL. OBJECTIVE: To analyze the effects of pre-operative WL on insulin resistance- and NAFLD-related histology in individuals undergoing bariatric surgery (BS) with or without pre-operative WL. DESIGN AND SETTING: A matched cross-sectional study was conducted at a public university hospital and a private clinic in Campinas, Brazil. METHODS: An analytical, observational, cross-sectional study was conducted using prospectively collected databases of individuals who underwent BS and liver biopsy at either a public tertiary university hospital (with pre-operative WL) or a private clinic (without pre-operative WL). Random electronic matching by gender, age, and body mass index (BMI) was performed and two paired groups of 24 individuals each were selected. RESULTS: Of the 48 participants, 75% were female. The mean age was 37.4 ± 9.6. The mean BMI was 38.9 ± 2.6 kg/m2. Fibrosis was the most common histopathological abnormality (91.7%). Glucose was significantly lower in the WL group (92 ± 19.1 versus 111.8 ± 35.4 mg/dL; P = 0.02). Significantly lower frequencies of macrovesicular steatosis (58.3% versus 95.8%; P = 0.004), microvesicular steatosis (12.5% versus 87.5%; P < 0.001), and portal inflammation (50% versus 87.5%; P = 0.011) were observed in the WL group. CONCLUSION: Pre-operative WL was significantly associated with lower frequencies of macro- and mi- crovesicular steatosis, portal inflammation, and lower glycemia, indicating an association between the recent trajectory of body weight and histological aspects of NAFLD.

Resumen Introducción: los lactantes que reciben lactancia materna completa (LMC) regulan su apetito de manera diferente a los que reciben sucedáneos de la leche humana (SLH). Además, la exposición temprana al colesterol en la leche humana conduciría a mejor regulación del colesterol en etapas posteriores de la vida. El propósito fue de comparar el perfil lípidos en lactantes de cuatro meses y correlacionarlo con indicadores antropométricos y hormonas reguladoras del apetito según el tipo de alimentación. Métodos: en un estudio transversal y correlacional se incluyeron 145 díadas madre-lactante según el tipo de alimentación; 64 recibieron LMC, 47 lactancia materna parcial (LMP) y 34 SLH. Se midió el perfil lipídico, grelina total, leptina, péptido YY y péptido tipo 1 similar al glucagón. Se obtuvieron puntajes Z para peso/edad, longitud/edad, peso/longitud, pliegue cutáneo tricipital y subescapular e índice de masa corporal para la edad. Resultados: hubo diferencias significativas en triglicéridos y colesterol LDL según el tipo de alimentación. En el grupo HMS se observó una relación inversa entre grelina y triglicéridos (p = 0,038), grelina y colesterol total (TC) (p = 0,026), y péptido YY y colesterol HDL (p = 0,017). En el grupo PBF hubo relación directa entre longitud/edad (z) y triglicéridos (p = 0,001) y entre pliegues subescapulares y CT (p = 0,049). En los lactantes que recibieron HMS, se observó una correlación directa entre peso/edad (z) y CT (p = 0,045) y entre longitud/edad (z) y colesterol LDL (p = 0,010). Conclusión: los hallazgos muestran una relación entre perfil lipídico, crecimiento, reserva energética y modulación de las hormonas reguladoras del apetito según el tipo de alimentación.

Abstract Background: infants receiving full breastfeeding (FBF) regulate their appetites differently from those receiving human milk substitutes (HMS). In addition, early exposure to the dietary cholesterol in human milk could lead to better cholesterol regulation in later stages of life. Therefore, the purpose was to compare lipid profiles in 4-month-old infants and to correlate lipid profile with anthropometric indicators and appetite-regulating hormones according to the type of feeding. Methods: this was a cross-sectional and correlational study, which included 145 mother-infant dyads according to the type of feeding; 64 received FBF, 47 partial breastfeeding (PBF), and 34 HMS. The complete lipid profile, total ghrelin, leptin, peptide YY, and glucagon-like peptide type 1 were measured. Z-scores for weight/age, length/age, weight/length, triceps (TSF) and subscapular folds (SSF) and body mass index for age were also obtained. Results: there were significant differences in triglycerides and LDL cholesterol according to the type of feeding. In the HMS group, an inverse relationship was observed between ghrelin and triglycerides (p = 0.038), ghrelin and total cholesterol (TC) (p = 0.026), and peptide YY and HDL cholesterol (p = 0.017). In the PBF group, a direct relationship was observed between length/age (z) and triglycerides (p = 0.001) and between subscapular folds and TC (p = 0.049). In infants receiving HMS, a direct correlation was observed between weight/age (z) and TC (p = 0.045) and beFfigtween length/age (z) and LDL cholesterol (p = 0.010). Conclusion: these findings show a relationship between growth, energy reserve, lipid profile, and modulation of appetite-regulating hormones according to the type of feeding they received.

Abstract Introduction: Urinary catheter-related infection is commonly associated with bacterial biofilm. The impact of anaerobes is unknown, but their detection in the biofilm on this device has not been previously reported. This study aimed to evaluate the capability to recovery strict, facultative, and aerobic microorganisms in patients using bladder catheters from ICUs using conventional culture, sonication, urinary analysis, and mass spectrometry. Methods: Parallel, sonicated bladder catheters from 29 critically ill patients were compared with their routine urine culture. Identification was performed using matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry. Results: The positivity rate in urine (n = 2, 3.4%) was lower than that in sonicated catheters (n = 7, 13.8%). Conclusion: Bladder catheter sonication showed more positive culture results than urine samples for anaerobic and aerobic microorganisms. The role of anaerobes in urinary tract infection and catheter biofilm is discussed.

Resumo Introdução: A infecção relacionada ao cateter urinário é comumente associada ao biofilme bacteriano. O impacto dos anaeróbios é desconhecido, mas sua detecção no biofilme deste dispositivo não foi relatada anteriormente. Este estudo teve como objetivo avaliar a capacidade de recuperar microrganismos estritos, facultativos e aeróbios em pacientes que utilizam cateteres vesicais de UTIs utilizando cultura convencional, sonicação, análise urinária e espectrometria de massa. Métodos: Paralelamente, foram comparados cateteres vesicais sonicados de 29 pacientes gravemente enfermos com sua urocultura de rotina. A identificação foi realizada utilizando dessorção/ionização a laser assistida por matriz com espectrometria de massa por tempo de voo. Resultados: A taxa de positividade na urina (n = 2; 3,4%) foi inferior à dos cateteres sonicados (n = 7; 13,8%). Conclusão: A sonicação do cateter vesical apresentou resultados de cultura mais positivos do que as amostras de urina para microrganismos anaeróbios e aeróbios. É discutido o papel dos anaeróbios na infecção do trato urinário e no biofilme do cateter.

ABSTRACT Due to its excellent resistance to traction and compression, wood is regularly used in furniture, window frames and in structures and sealing of homes and buildings. Due to the anisotropy and complexity of the material, its properties vary depending on its intrinsic anatomical characteristics and external factors, such as species, moisture content, acting requests, among others. The Brazilian standard provides linear models for estimating and correcting all strength and stiffness properties as a function of the moisture content of the wood. However, due to its characteristics, there may not be a linear variation of the physical and mechanical properties in relation to the moisture content. Thus, this research aims to evaluate and analyze the influence of moisture content on 15 properties of Calophyllum sp. The reliability of the Brazilian standard equation for correcting strength and stiffness values as a function of humidity (U% to 12%) was evaluated, in addition to analyzing, through ANOVA, whether there is a change in the properties when they are at the saturation point of the fiber and 12% moisture. Of the 15 properties analyzed, 4 did not change, contrary to the linear models set out in the Brazilian standard. In addition, it was possible to note that the Brazilian standard can estimate with an error of up to 76% when correcting the humidity to 12%.

RESUMO A madeira por possuir excelente resistência à tração e compressão é comumente utilizada em móveis, esquadrias e em estruturas e vedação de residências e edifícios. Devido à anisotropia e complexidade do material, suas propriedades variam em função de suas características anatômicas intrínsecas e de fatores externos, como a espécie, teor de umidade, solicitações atuantes, dentre outros. A norma brasileira traz modelos lineares para a estimativa e correção de todas as propriedades de resistência e de rigidez em função do teor de umidade da madeira. Porém, devido às suas características, pode não haver variação linear das propriedades físicas e mecânicas em relação ao teor de umidade. Assim, esta pesquisa tem como objetivo avaliar e analisar a influência do teor de umidade em 15 propriedades da madeira Calophyllum sp. Foi avaliada a confiabilidade da equação da norma brasileira para corrigir os valores de resistência e rigidez em função da umidade (U% para 12%), além de analisar, por meio de ANOVA, se há alteração nas propriedades quando estão no ponto de saturação da fibra e 12% de umidade. Das 15 propriedades analisadas, 4 não sofreram alteração, contrariando os modelos lineares dispostos na norma brasileira. Além disso, foi possível notar que a norma brasileira pode estimar com erro de até 76% ao corrigir a umidade para 12%.

Resumen La necesidad de investigar y evaluar la alternativa de producir triticale en México, para luego, incorporarlo a la cadena agroalimentaria en la alimentación humana es importante, debido a su contenido de proteína (alrededor de 20 % más que el trigo), sumado al mejor balance de aminoácidos y a su grano más rico en fósforo que el grano de trigo. El objetivo de esta investigación fue identificar líneas de triticales (X. Triticosecale Wittmack) con base en las propiedades físicas y químicas del grano con valor para la industria alimentaria. Se evaluaron 20 líneas élite de triticales primaverales del programa de investigación del CIMMYT (Centro Internacional de Mejoramiento de Maíz y Trigo). El estudio se realizó en 2017 en Celaya, Guanajuato, México. El experimento se aleatorizó en un diseño experimental completamente al azar con seis repeticiones, cada unidad experimental correspondió a cada línea, las variables medidas fueron: peso de mil granos (PMG) peso hectolítrico (PH), humedad, cenizas, grasa, fibra, proteína y carbohidratos fueron determinadas en el laboratorio de análisis de alimentos del TecNM-Roque. Los resultados mostraron diferencias estadísticas entre líneas; se encontraron mayores contenidos de PMG (45.85 g), PH (78.8 kg hL-1) en L-18 y L-20 respectivamente, en grasa, fibra, proteínas y carbohidratos las líneas L-17, L-18 y L-20 obtuvieron mayores promedios, en cenizas el 75 % de las líneas mostraron valores inferiores al 2.0 %.

Abstract The need to investigate and evaluate the alternative of producing triticale in Mexico, to later incorporate it into the agri-food chain for human consumption is important, due to its good protein content (around 20 % more than wheat), added to the better balance of amino acids and its grain is richer in phosphorus than wheat grain. The objective of this research was to identify lines of triticales (X. Triticosecale Wittmack) based on the physical and chemical properties of the grain with value for the food industry. Twenty elite lines of spring triticales from the CIMMYT (International Maize and Wheat Improvement Center) research program was evaluated. The study was carried out in 2018 in Celaya, Guanajuato, Mexico, the experiment was randomized in a completely randomized experimental design with six repetitions, each experimental unit was each line, the variables measured were: thousand grain weight (PMG) hectoliter weight (PH), humidity, ashes, fat, fiber, protein and carbohydrates were determined in the food analysis laboratory of TecNM-Roque. The results showed statistical differences between lines; higher contents of PMG (45.85 g), PH (78.8 Kg hL-1) were found in L-18 and L-20 respectively, in fat, fiber, carbohydrate proteins the lines L-17, L-18 and L-20 obtained higher averages, in ash 75 % of the lines showed values lower than 2.0 %.

The present work focuses on a theoretical/bibliographical discussion that will investigate the academic production of articles centered on teaching practices guided by an anti-racist education and on the implications of these actions in the school as a public institution for the formation of social subjects. The objective is to amalgamate in the work, from the analysis of the selected scientific productions, the educational potentialities and obstacles faced in the school. It is understood that such works can reverberate in the awareness of teachers and encourage anti-racist practices from the school, which is invested with greater importance when we are faced with a Brazilian society marked by the existence and perpetuity of structural racism. Therefore, when researching anti-racist educational practices and the school as a school institution, inserted in a contemporary social context, an anti-racist potential is evident from the social reproduction in the dialogue between the historical structure and current contextual practices. Moreover, obstacles are found when the historical dimension is erased from the contemporary system of practices.

El presente trabajo se centra en una discusión teórico/bibliográfica que investigará la producción académica de artículos centrados en las prácticas docentes orientadas por una educación antirracista y en las implicaciones de estas acciones en la escuela como institución pública para la formación de sujetos sociales. El objetivo es amalgamar en el trabajo, a partir del análisis de las producciones científicas seleccionadas, las potencialidades educativas y los obstáculos enfrentados en la escuela. Se entiende que tales obras pueden repercutir en la conciencia de los docentes y fomentar prácticas antirracistas desde la escuela, lo que adquiere mayor importancia cuando nos enfrentamos a una sociedad brasileña marcada por la existencia y perpetuidad del racismo estructural. Por lo tanto, al investigar las prácticas educativas antirracistas y la escuela como institución escolar, inserta en un contexto social contemporáneo, se evidencia un potencial antirracista desde la reproducción social en el diálogo entre la estructura histórica y las prácticas contextuales actuales. Además, se encuentran obstáculos cuando se borra la dimensión histórica del sistema de prácticas contemporáneo.

O presente trabalho tem como foco uma discussão de cunho teórico/bibliográfico que vai investigar a produção acadêmica de artigos com foco em práticas docentes pautadas por uma educação antirracista e nas implicações dessas ações na escola como instituição pública de formação dos sujeitos sociais. A expectativa é de amalgamar no trabalho, minimamente, as possíveis potencialidades educativas e obstáculos enfrentados na escola e que tenham sido abordados por trabalhos publicados em uma importante plataforma digital de divulgação de pesquisas acadêmicas. Entendemos que tais trabalhos podem reverberar na sensibilização de docentes e fomentar práticas antirracistas no chão da escola, o que se investe de uma maior importância ao nos deparar com uma sociedade brasileira marcada pela existência e perenidade do racismo estrutural. Diante disso, ao pesquisar práticas educativas antirracistas e a escola como instituição escolar, inserida em uma conjuntura social contemporânea.

Abstract: Introduction: gastrointestinal stromal tumors are the most common mesenchymal tumors, they can occur anywhere in the digestive tract. Objective: to present the experience of 10 years in the Oncology Unit of the General Hospital of Puebla “Dr. Eduardo Vázquez Navarro” in the management of patients diagnosed with Gastrointestinal stromal tumors. Material and methods: longitudinal, retrospective, observational and clinical study carried out in patients with a histopathological diagnosis of Gastrointestinal stromal tumors between the months of January 2012 to December 2022 in the Oncology Unit of the General Hospital of Puebla. Discussion: 24 records of patients with a diagnosis of Gastrointestinal stromal tumors tumor were reviewed. An institutional incidence of 2% was found. The average age at diagnosis was 53 years, with a male predominance. Surgical treatment was performed in 96% of the cases. A 5-year survival of 16% was found. Recurrence occurred in 16% to liver and lung. Conclusions: gastrointestinal stromal tumors have an unpredictable behavior. Surgery in early stages is the curative treatment. Its indolent presentation makes its diagnosis difficult until advanced stages of the disease. Tyrosine kinase inhibitors have improved survival and are a therapeutic option in cases where surgery is not possible.

Resumen: Introducción: los tumores del estroma gastrointestinal son los tumores mesenquimales más comunes y pueden presentarse en cualquier parte del tracto digestivo. Objetivo: exponer la experiencia de 10 años en la Unidad de Oncología del Hospital General de Puebla “Dr. Eduardo Vázquez Navarro” en el manejo de pacientes con diagnóstico de tumores del estroma gastrointestinal. Material y métodos: estudio longitudinal, retrospectivo, observacional y clínico efectuado en pacientes con diagnóstico histopatológico de tumores del estroma gastrointestinal entre los meses de enero de 2012 y diciembre de 2022 en la Unidad de Oncología del Hospital General de Puebla. Discusión: se revisaron 24 expedientes de pacientes con diagnóstico de tumor del estroma gastrointestinal. Se observó una incidencia institucional de 2%. El promedio de edad al momento del diagnóstico fue de 53 años, con predominio masculino. Se realizó tratamiento quirúrgico en 96% de los casos. Se encontró una supervivencia a cinco años de 16% y se presentó recurrencia en 16% en hígado y pulmón. Conclusiones: los tumores del estroma gastrointestinal tienen un comportamiento impredecible. La cirugía en etapas tempranas es el tratamiento curativo. Su presentación indolente dificulta su diagnóstico hasta etapas avanzadas de la enfermedad. Los inhibidores de tirosina cinasa han mejorado la supervivencia y son una opción terapéutica en casos en los que no es posible realizar la cirugía.

Abstract The present study aimed to evaluate bacterial viability after the use of different disinfection protocols in root canals infected with a multispecies biofilm (MB) formed in situ. Palatal roots with a single canal were obtained from extracted maxillary molars and sterilized before being inserted into the mouth. The roots were contaminated with a MB in an intraoral appliance worn by ten volunteers. All volunteers wore six roots simultaneously in two intraoral devices for 21 days. One root from each volunteer was assigned to each group (n=10): PUI - passive ultrasonic irrigation; EC - Easy Clean; XPF - XP-endo Finisher; aPDT - antimicrobial photodynamic therapy; CI - conventional irrigation; and NC - negative control. The samples were evaluated under confocal laser scanning microscopy. The percentage of viable cells (VC) was calculated over the total percentage of MB biovolume. Data were statistically analyzed (α=5%). The cell viability in the entire root canal or for each third was compared between groups (Kruskal-Wallis test, Dunn post-hoc test) and for the same group (Friedman test, Dunn post-hoc test). Disinfection protocols were not significantly different from each other (P>.05). Samples in EC, PUI, and aPDT had lower cell viability than in NC (P<.05). In the coronal third of samples in the EC, XPF, PUI and aPDT, the percentage of VC biovolume was lower than in the NC (P<.05). The percentage of VC in EC samples was lower in the coronal and middle thirds than in the apical third (P<.05). EC, PUI and aPDT had significant effects on cell viability in intraradicular multispecies biofilm formed in situ when compared with untreated samples.

Resumo O presente estudo teve como objetivo avaliar a viabilidade bacteriana após o uso de diferentes protocolos de desinfecção em canais radiculares infectados com um biofilme multiespécies (MB) formado in situ. Raízes palatinas com canal único foram obtidas de molares superiores extraídos e esterilizadas antes de serem inseridas na boca. As raízes foram contaminadas com MB em um aparelho intraoral usado por dez voluntários. Todos os voluntários usaram seis raízes simultaneamente em dois dispositivos intrabucais por 21 dias. Uma raiz de cada voluntário foi atribuída a cada grupo (n=10): PUI - irrigação ultrassônica passiva; EC - Easy clean; XPF - XP-endo Finisher; aPDT - terapia fotodinâmica antimicrobiana; IC - irrigação convencional; e, NC - controle negativo. As amostras foram avaliadas em microscopia confocal de varredura a laser. A porcentagem de células viáveis (VC) foi calculada sobre a porcentagem total do biovolume de MB. Os dados foram analisados estatisticamente (α=5%). A viabilidade celular em todo o canal radicular ou em cada terço foi comparada entre os grupos (teste de Kruskal-Wallis, teste post-hoc de Dunn) e no mesmo grupo (teste de Friedman, teste post-hoc de Dunn). Os protocolos de desinfecção não foram significativamente diferentes entre si (P>0,05). Amostras dos grupos EC, PUI e aPDT apresentaram menor viabilidade celular do as do NC (P<0,05). No terço cervical das amostras do EC, XPF, PUI e aPDT, a porcentagem de biovolume de VC foi menor do que no NC (P<0,05). A porcentagem de VC nas amostras do EC foi menor nos terços cervical e médio do que no terço apical (P<0,05). EC, PUI e aPDT tiveram efeitos significativos na viabilidade celular do biofilme multiespécies intrarradicular formado in situ quando comparado com amostras não tratadas. Estudos clínicos devem investigar o papel da redução de cargas bacterianas viáveis no sistema de canais radiculares para o sucesso do tratamento endodôntico.

Resumen El objetivo de esta investigación fue identificar líneas de triticale (X. Triticosecale Wittmack) de valor en la industria alimentaria, con base en sus propiedades físicas y químicas. Veinte líneas élite de triticale primaveral del programa de investigación del CIMMyT se establecieron bajo un diseño experimental completamente al azarcón seis repeticiones durante 2018 en Celaya, Guanajuato, México, donde se evaluaron las variables: PMG, PH, humedad, cenizas, grasa, fibra, proteína y carbohidratos. Los resultados mostraron diferencias (p≤ 0.05) entre líneas, los mayores valores de PMG, PH y porcentaje superior en grasa, fibra, proteínas y carbohidratos se encontraron en las líneas L-18, L-17 y L-14, además de L-20, L-10 y L-19. El modelo AMMI detectó diferencias significativas (p≤ 0.05) para las líneas y sus interacciones con las variables fisicoquímicas; el gráfico biplot mostró que las líneas L-18, L-7, L-10, L-17 y L-20 expresaron mayor asociación con PMG, PH, proteínas, grasas, fibra y carbohidratos, lo que les permite considerarlas en el uso de la industria alimentaria por obtener promedios superiores.

Abstract The objective of this research was to identify lines of triticale (X. Triticosecale Wittmack) of value in the food industry, based on their physical and chemical properties. Twenty elite lines of spring triticale from the CIMMYT research program were established under a completely randomized experimental design with six repetitions during 2018 in Celaya, Guanajuato, Mexico, where the following variables were evaluated: WTG, HW, moisture, ashes, fat, fiber, protein, and carbohydrates. The results showed differences (p≤ 0.05) between lines, the highest values of WTG, HW and the highest percentage in fat, fiber, proteins and carbohydrates were found in lines L-18, L-17 and L-14, in addition to L-20, L-10 and L-19. The AMMI model detected significant differences (p≤ 0.05) for the lines and their interactions with physicochemical variables; the biplot graph showed that lines L-18, L-7, L-10, L-17 and L-20 expressed greater association with WTG, HW, proteins, fats, fiber and carbohydrates, which allows them to be considered in the use of the food industry for obtaining higher averages.

ABSTRACT The application of organic amendments is an increasingly widespread practice, which allows fertilizing and restoring soils in a sustainable and environmentally friendly way. Improving the quality of amendments through the application of microorganisms has been a challenge in recent years. The objective of the study was to determine the effects of microalgae+compost (1:1) mixture on the yield of cauliflower (Brassica oleracea L. var. botrytis L.) plants grown in a sandy loam soil with low nutrient availability. Yields were compared with the application of compost, cattle manure and two chemical fertilizers. The treatments applied were: T1 prepared with urea (NPK 46-0-0), triple superphosphate (NPK 0-46-0) and potassium saltpeter (NPK 15-0-14); T2 commercial fertilizer (NPK 12-11-18); T3 microalgae biomass (T3); T4 compost; T5 1:1 microalgae+compost mixture; T6 cattle manure; and T0 control (no fertilization). The experiments were conducted in 30 L pots using 40 g soil per plant and 4 g N as a reference to standardize fertilizer application. The results showed that the fresh weight (FW) and dry weight (%DW) of T5 and T6 were not significantly different from those of T1 (p > 0.05), on the contrary, FW of T5 and T6 was 43.8% and 40% higher than in T2, as well as the %DW was 0.59% and 0.6% w w-1, also higher than in T2. These results suggest that the microalgae+compost mixture is an alternative fertilizer, equivalent to chemical fertilizers and manure, presenting the advantages of being more stable, sustainable, and environmentally friendly. However, further studies are needed to determine the necessary dose per cultivated plant species.

Abstract Shear strength parallel to the fibers (f v0 ) is extremely important for the design of timber structures. Then, due to the lack of information related to a possible variation in the f v0 of hardwood species according to the load application plane in relation to its growth rings/rays, this work performed tests for the determination of f v0 according to NBR 7190-3 (ABNT, 2022b) varying load planes. Thereby, the load was applied on the longitudinal-tangential (LT) and longitudinal-radial (LR) planes of the species of caixeta (Simarouba amara) and roxinho (Peltogyne spp.). Through variance analysis (5% confidence), results did not point to statistical differences between f v0 values when the load plane was changed. Therefore, the f v0 of this species can be determined without significant risk of reduction according to ray/ring orientation. Furthermore, it was determined a ratio of f v0,k /f c0,k equal to 0.23 and 0.38 was determined for roxinho and caixeta, respectively. Thus, this result confirms the need for characterizing unfamiliar wood species when received on construction site, in order to save money and/or for safety reasons.

Resumo Aresistência ao cisalhamento paralelo às fibras (fv0) é de extrema importância no projeto de estruturas de madeira. Logo, devido à falta de informações a respeito de uma possível variação na fv0 de espécies folhosas de acordo com o plano de aplicação de carga em relação a seus anéis de crescimento/raios, este trabalho realizou ensaios para a determinação da fv0 de acordo com a NBR 7190-3 (ABNT, 2022b), variando-se planos de carga. O carregamento foi aplicado no plano longitudinal tangencial (LT) e longitudinal radial (LR) das espécies de caixeta (Simarouba amara) e roxinho (Peltogyne spp.). Por meio da análise de variância (5% de significância), os resultados não apontaram diferença estatística entre os valores da fv0 quando se variou o plano de carga. Portanto, a fv0 dessas espécies pode ser determinada sem que haja risco significativo de minoração de acordo com a disposição dos raios/anéis. Além disso, foi determinada uma relação de fv0,k/fc0,k igual a 0,23 e 0,38 para o roxinho e a caixeta, respectivamente. Assim, esse resultado corrobora a necessidade de se caracterizar a madeira de espécie pouco conhecida quando recebida na obra, de modo a gerar economia e/ou segurança.

Abstract The objective of this study was to identify in Angus and Brangus breed animals with extreme temperament, measured as exit velocity, genomic regions and candidate genes associated with bovine temperament. The population was genotyped with the Genomic Profiler HD 150K chip and after the genome-wide association analysis, the SNPs rs133956611 (P=2.65 E-06) and rs81144933 (P=9.58 E-06) were associated with temperament. The mapping analysis of the regions close to the SNP rs81144933 identified the SNCA (alpha-synuclein) and MMRN1 (multimerin-1) genes at 222.8 and 435.9 Kb downstream respectively, while for the rs133956611 loci the gene GPRIN3 (GPRIN family-member-3) was identified at 245.7 Kb upstream, all three genes are located on the BTA6 chromosome. The analysis of SNCA protein-protein interactions allowed the identification of the genes APP (β-amyloid precursor protein), PARK7 (parkinsonism-associated-deglycase), UCHL1 (ubiquitin-C-terminal-hydrolase-L1), PARK2 (parkin-RBR- E3-ubiquitin-protein-ligase), and genes of the SLC family as candidates to be associated with bovine temperament. All these candidate genes and their interacting were resequenced, which allowed the discovery of new SNPs in the SNCA and APP genes. Of these, the SNPs located in introns 5, 8 and 11 of the APP gene affect splicing site motifs. These results indicate that SNCA and its interacting genes are candidates to be related to bovine temperament.

Resumen El objetivo de este estudio fue identificar en animales de raza Angus y Brangus con temperamento extremo, medido como velocidad de salida, regiones genómicas y genes candidatos asociados con el temperamento bovino. La población fue genotipada con el chip Genomic Profiler HD 150K y después del análisis de asociación del genoma completo, los SNP rs133956611 (P= 2.65 E-06) y rs81144933 (P= 9.58 E-06) se asociaron con el temperamento. El análisis de mapeo de las regiones cercanas al SNP rs81144933 identificó los genes SNCA (alfa-sinucleína) y MMRN1 (multimerin-1) a 222.8 y 435.9 Kb corriente abajo, respectivamente, mientras que para los loci rs133956611 se identificó el gen GPRIN3 (familia GPRIN- miembro 3) a 245.7 Kb corriente arriba, los tres genes se encuentran en el cromosoma BTA6. El análisis de las interacciones proteína-proteína de SNCA permitió la identificación de los genes APP (proteína precursora de β-amiloide), PARK7 (deglicasa asociada al parkinsonismo), UCHL1 (ubiquitina C-terminal hidrolasa L1), PARK2 (parkina-RBR-E3-ubiquitina-proteína-ligasa), y genes de la familia SLC como candidatos a estar asociados con el temperamento bovino. Todos estos genes candidatos y su interacción fueron resecuenciados, lo que permitió el descubrimiento de nuevos SNP en los genes SNCA y APP. De estos, los SNP localizados en los intrones 5, 8 y 11 del gen APP afectan a los motivos del sitio de empalme. Estos resultados indican que el SNCA y sus genes interactuantes son candidatos para estar relacionados con el temperamento bovino.

Resumen Descripción del caso clínico: Se describe la etiología, cuadro clínico y diagnóstico de un paciente con carcinoma primario de uretra bulbar con antecedente de cáncer de próstata tratado mediante prostatectomía radical robot-asistida, siendo ahora realizada cistectomía más conducto ileal intracorpóreo por laparoscopía más uretrectomía total. Relevancia: Pocos casos reportados en la literatura médica y el manejo realizado en nuestro centro en donde observamos los beneficios de la cirugía por mínima invasión en casos previamente intervenidos quirúrgicamente. Implicaciones clínicas: La importancia del seguimiento oncológico estrecho y el beneficio en la evolución clínica del paciente posterior a su intervención por mínima invasión. Conclusiones: Todo paciente con diagnóstico oncológico debe ser vigilado de forma estrecha y con adecuado apego con la finalidad de detectar posibles recurrencias de la enfermedad o aparición de nueva enfermedad, siempre valorando el manejo por mínima invasión para disminuir la morbimortalidad en cada paciente.

Abstract Case description: It describes the etiology, clinical scenario, and diagnosis of a patient with primary bulbar urethral carcinoma with history of a prostate cancer treated by Robot-assisted radical prostatectomy, actually treated with cystectomy, intracorporeal ileal conduit by laparoscopy, and total urethrectomy. Relevance: Very few reported cases in medical literature and the important benefits of the minimally invasive surgery we observed in our work center in patients with a history of surgical interventions. Clinical implications: Importance of narrow oncological surveillance and the benefit in postoperative recovery of a patient treated by minimal invasive procedures. Conclusions: Every patient with an oncological diagnosis must have a narrow follow to detect the recurrence of the primary disease or new disease in a different place and if possible, always offer our patient a minimally invasive approach to diminish morbidity and mortality rate.