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ABSTRACT The symbiosis of common beans with nitrogen-fixing bacteria provides an efficient approach to sustainable and economical food production. Therefore, this study aimed to evaluate the application of cost-effective nitrogen management strategies in organic common bean cultivation, including the application of poultry manure and organic liquid fertilizer, seed and co-inoculation with diazotrophic bacteria (Azospirillum brasilense and/or Rhizobium tropici), and supplementary Rhizobium tropici inoculation. The study spanned three years in a well-established organic cultivation field (2018) and an initial organic cultivation area (2019 and 2020) in Brazil. It was arranged in a randomized blocks design in a 2 × 5 (2018) and 2 × 6 (2019 and 2020) factorial scheme, with four replicates. The most profitable strategy involves seed inoculation with Azospirillum brasilense and additional inoculation with Rhizobium tropici at stage V4-5 (fifth trifoliate leaf fully expanded), while the most costly strategy was the application of poultry manure at the V3 stage (first trifoliate leaf fully expanded). Thus, the use of diazotrophic bacteria for seed inoculation and co-inoculation (Azospirillum brasilense and/or Rhizobium tropici) ensured financial returns and system profitability in common bean cultivation.

RESUMO A simbiose do feijão comum com bactérias fixadoras de nitrogênio proporciona uma abordagem eficiente para a produção de alimentos sustentável e econômica. Portanto, este estudo teve como objetivo avaliar a aplicação de estratégias custo-efetivas de manejo de nitrogênio no cultivo orgânico do feijão comum, incluindo a aplicação de esterco de aves e fertilizante orgânico líquido, inoculação de sementes e coinoculação com bactérias diazotróficas (Azospirillum brasilense e/ou Rhizobium tropici), e inoculação suplementar com Rhizobium tropici. O estudo durou três anos em um campo de cultivo orgânico bem estabelecido (2018) e uma área inicial de cultivo orgânico (2019 e 2020) no Brasil. O delineamento foi em blocos casualizados em esquema fatorial 2 × 5 (2018) e 2 × 6 (2019 e 2020), com quatro repetições. A estratégia mais lucrativa envolve a inoculação de sementes com Azospirillum brasilense e inoculação adicional com Rhizobium tropici no estádio V4-5 (quinta folha trifoliada totalmente expandida), enquanto a estratégia mais cara foi a aplicação de esterco de aves no estádio V3 (primeira folha trifoliada totalmente expandida). Assim, o uso de bactérias diazotróficas para inoculação e coinoculação de sementes (Azospirillum brasilense e/ou Rhizobium tropici) garantiu retorno financeiro e rentabilidade do sistema no cultivo do feijão comum.

https://doi.org/10.1590/1807-1929/agriambi.v28n2e272029

Phytochemical profile and biological activities of Bromelia antiacantha extracts
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Rodrigues, K. F.

; Bitencourt, T. C.

; Núñez, J. G.

; Garcia, H. O.

; Buhl, B.

; Padilha, G. L.

; Ethur, E. M.

; Hoehne, L.

; Bruno, A. N.

; Freitas, E. M.

Brazilian Journal of Biology

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2024, Volume 84 elocation e255529

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Relatos da medicina popular costumam atuar como referencial para o desenvolvimento de novos fármacos a partir de moléculas naturais com ações terapêuticas para doenças de alta gravidade e prevalência como o câncer. Bromelia antiacantha Bertol. é uma espécie da família Bromeliaceae, considerada uma planta alimentícia não convencional (PANC), encontrada nas regiões sul e centro-oeste do Brasil. Apesar do alto teor nutritivo e potencial farmacológico de seus frutos, poucos estudos científicos relatam suas ações biológicas. Desta forma, este estudo avalia o perfil fitoquímico de extratos aquoso e etanólico obtidos de frutos de B. antiacantha, bem como a sua possível ação antioxidante, antitumoral e citotóxica. O extrato aquoso apresentou compostos fenólicos e flavonoides, enquanto os extratos etanólicos apontam a presença de flavonóides e cumarina em sua composição, independente da região de coleta. O extrato etanólico demonstrou efeito antioxidante mais promissor do que o extrato aquoso e também induziu uma inibição significativa na viabilidade de células humanas de câncer cervical da linhagem SiHa. Além disso, o tratamento com ambos extratos não alterou a viabilidade de células não tumorais da linhagem de queratinócitos humanos imortalizados (HaCaT). Estes dados trazem novas informações sobre extratos obtidos de uma espécie vegetal nativa, comestível e já utilizada tradicionalmente, mas abrindo novas perspectivas quanto a possíveis aplicações terapêuticas.

Abstract Reports from popular medicine usually act as a basis for the development of new drugs from natural compounds with therapeutic actions for serious diseases and prevalence such as cancer. Bromelia antiacantha Bertol. is a species of the Bromeliaceae family, considered an unconventional food plant, found in the south and midwest regions of Brazil. Despite the high nutritional content and pharmacological potential of its fruits, few scientific studies report its biological actions. Thus, this study evaluates the phytochemical profile of aqueous and ethanol extracts obtained from B. antiacantha fruits, as well as their possible antioxidant, antitumor, and cytotoxic activities. The aqueous extract exhibited phenolic compounds and flavonoids, while ethanol extracts indicated the presence of flavonoids and coumarin in their composition, regardless of the region of collection. The ethanolic extract demonstrated a more promising antioxidant effect than the aqueous extract and also induced a significant inhibition in the viability of human cervical cancer cells of the SiHa strain. In addition, treatment with both extracts did not alter the viability of non-tumor cells of the immortalized human keratinocyte lineage (HaCaT). These results bring new data about extracts obtained from a native plant, edible and traditionally used in popular medicine, opening new perspectives for its possible therapeutic application.

https://doi.org/10.1590/1519-6984.255529

Impact of adding different concentrations of IGF-I and insulin to the semen extender on bull sperm quality post-cryopreservation
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Pinto, S.C.C.

; Souza, F.A.

; Arruda, R.P.

; Alves, M.B.R.

; Batissaco, L.

; Garcia-Oliveros, L.N.

; Passarelli, M.S.

; Carneiro, L.C.

; Celeghini, E.C.C.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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oct 2023, Volume 75 N. 5 Pages 771 - 786

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT This study aimed to evaluate the addition of different concentrations of IGF-I and insulin to egg yolk-based extender to improve bovine semen cryopreservation. Two experiments were developed to evaluate the effects of the additives in two commercial extenders, Botubov® (Experiment 1) and Triladyl® (Experiment 2), both with the same design. Three ejaculates from four bulls (n = 12) were used. Each ejaculate was divided into seven equal fractions for dilution (60x106 spermatozoa/mL) in the following treatments: CON: extender only; IGF100: IGF-I 100ng/mL; IGF200: IGF-I 200ng/mL; INS150: insulin 150µUI/mL; INS200: insulin 200µUI/mL; ASS1: IGF-I 100ng/mL + insulin 150µUI/mL; ASS2: IGF-I 200ng/mL + insulin 200µUI/mL. Semen was cryopreserved by an automated system. Post-thawed sperm were evaluated regarding motility by CASA (Computer-assisted sperm analysis), and membranes by fluorescent probes (H342, PI, FITC-PSA and JC-1). For Botubov® extender, INS150 was more efficient in preserving total and progressive motility, VCL, BCF, plasma and mitochondrial membranes. A similar response was seen when insulin was added to the Triladyl® extender, INS150 was more efficient in preserving sperm motility, plasma membrane integrity and mitochondrial potential. Thus, the addition of insulin 150µUI/mL, regardless of the composition of the extender, contributes to better preserving bovine sperm from the cryopreservation effects.

RESUMO Este estudo teve o objetivo de avaliar a adição de diferentes concentrações de IGF-I e insulina a diluidores, à base de gema de ovo, para melhorar a criopreservação do sêmen bovino. Dois experimentos foram desenvolvidos para avaliar os efeitos dos aditivos em dois diluidores comerciais: Botubov® (Experimento 1) e Triladyl® (Experimento 2), ambos com o mesmo delineamento. Foram utilizados três ejaculados de quatro touros (n=12). Cada ejaculado foi dividido em sete frações para diluição (60x106espermatozoides/mL), nos seguintes tratamentos: CON: somente diluidor; IGF100: 100ng/mL de IGF-I; IGF200: 200ng/mL de IGF-I; INS150: 150µUI/mL de insulina; INS200: 200µUI/mL de insulina; ASS1: 100ng/mL de IGF-I + 150µUI/mL de insulina; ASS2: 200ng/mL de IGF-I + 200µUI/mL de insulina. O sêmen foi criopreservado por sistema automatizado. Após a criopreservação, o sêmen foi avaliado quanto à motilidade espermática por CASA e quanto às membranas espermáticas (plasmática, acrossomal e mitocondrial) por sondas fluorescentes (H342, PI, FITC-PSA e JC-1). Para o diluidor Botubov®, INS150 foi mais eficiente em preservar motilidades total e progressiva, VCL, BCF, integridade da membrana plasmática e potencial mitocondrial. Resposta semelhante foi observada quando a insulina foi adicionada ao diluidor Triladyl®, INS150 foi mais eficiente na preservação da motilidade, integridade das membranas e potencial mitocondrial quando comparado aos demais grupos. Assim, a adição de 150µUI/mL de insulina aos diluidores, independentemente da composição, contribui para melhor criopreservação dos espermatozoides bovinos.

https://doi.org/10.1590/1678-4162-12946

Physicochemical characterization of residual potato ( Solanum tuberosum) starch recovered from the potato chips industry in Mexico
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Montoya-Anaya, Diana G.

; Madera-Santana, Tomas J.

; Aguirre-Mancilla, Cesar L.

; Grijalva-Verdugo, Claudia

; Gonzales-Garcia, Gerardo

; Nuñez-Colín, Carlos A.

; Rodriguez-Nuñez, Jesus R.

Biotecnia

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aug 2023, Volume 25 N. 2 Pages 60 - 72

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen El objetivo de este estudio fue evaluar las propiedades fisicoquímicas, morfológicas, estructurales y térmicas del almidón recuperado de los residuos de Solanum tuberosum de la industria de frituras en México, utilizando técnicas instrumentales avanzadas. La papa residual se recogió de una empresa productora de papas fritas y el almidón se obtuvo mediante extracción acuosa. El almidón presentó un contenido de carbohidratos de 87.79 %, proteínas (1.18 %), lípidos (0.15 %), cenizas (0.26 %) y amilosa (24.35 %). La capacidad de absorción de agua fue de 1.0163 g g-1, solubilidad (30.17 - 46.65 %), hinchamiento (2.31 - 8.69 g g-1). Los análisis térmicos mostraron una gelatinización del almidón entre 61.7 - 90 °C con una pérdida máxima de peso a 288.1 °C. La microscopía electrónica de barrido-espectroscopía dispersiva de energía (SEM-EDS) mostró partículas de 14 - 69 µm con una composición de 54.3 % de carbono, 45.4 % de oxígeno y 0.15 % de potasio. La dispersión dinámica de luz (DLS) mostró partículas de tamaño nanométrico (120 nm). La resonancia magnética nuclear (NMR) y la difracción de rayos X (XRD) exhibieron la cristalinidad del almidón como tipo B (34.73 %) y la reflectancia total atenuada por espectroscopia infrarroja transformada de Fourier (FTIR - ATR) mostró los grupos funcionales distintivos de la glucosa.

Abstract The objective of this study was to evaluate the physicochemical, morphological, structural, and thermal properties of starch recovered from Solanum tuberosum residual of chips industry in Mexico using advanced instrumental techniques. The residual potato was collected from a chips production company and the starch obtained by aqueous extraction. The starch presented a content of carbohydrates of 87.79 %, proteins (1.18 %), lipids (0.15 %), ashes (0.26 %), and amylose (24.35 %). The water absorption capacity was 1.0163 g g-1, solubility (30.17 - 46.65 %), swelling (2.31 - 8.69 g g-1). Thermal analyzes showed a starch gelatinization about 61.7 - 90 ºC with a maximum weight loss at 288.1 °C. The scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) showed particles of 14 - 69 µm with a composition of 54.3 % carbon, 45.4 % oxygen, and 0.15 % potassium. The dynamic light scattering (DLS) showed particles of nanometric size (120 nm). The nuclear magnetic resonance (NMR) and X-Ray diffraction (XRD) exhibited the crystallinity of the starch as type B (34.73 %) and the Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) showed the functional groups distinctive of the glucose.

https://doi.org/10.18633/biotecnia.v25i2.1880

The use of seawater in copper hydrometallurgical processing in Chile: A review
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Astudillo, Á.

; Garcia, M.

; Quezada, V.

; Valásquez, L.

Journal of the Southern African Institute of Mining and Metallurgy

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jul 2023, Volume 123 N. 7 Pages 357 - 364

Abstract: En | Text: En | PDF: En

Seawater has become a viable alternative for different uses in copper hydrometallurgy. In this paper we review the main physical and chemical characteristics of seawater and how these influence copper production. Reliable data on the use of continental water are reported, and the current use and consumption of seawater in the Chilean mining industry is analysed, indicating the main areas of use and the main problems encountered. Additionally, the influence of the elements in seawaterthat have the most influence on the extractive metallurgy of copper are considered. The Chilean copper mining industry currently consumes approximately 4.1 m³/s of seawater, which corresponds to 25% of the total water used. The use of seawater for the leaching of copper sulphide minerals, such as chalcopyrite, is beneficial because it provides 20 g/L of chloride, thereby improving copper dissolution kinetics.

https://doi.org/10.17159/2411-9717/2445/2023

Nectarine Coated with Biopolymeric Nanocapsules Containing Eugenol to Control Brown Rot
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Jacumazo, Joslaine

; Parchen, Gabriela P.

; Garcia, Meira J. B.

; Santos, Nayana C. S.

; Mio, Louise L. M. De

; Marques, Francisco A.

; Freitas, Rilton A. de

Journal of the Brazilian Chemical Society

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jul 2023, Volume 34 N. 7 Pages 1030 - 1039

Abstract: En | Text: En | PDF: En

Infections in fruits caused by fungi reduce the quantity and quality of food for human consumption, in addition to causing economic losses. In this sense, this study aimed to address the effects of eugenol nanocapsules (NCs) based on chitosan and carboxymethylcellulose in protecting nectarines against Monilinia fructicola, a brown rot agent, a worldwide important disease. NCs were prepared by layer-by-layer (LbL) self-assembly starting from an anionic template and deposition with up to two polymeric layers. The hydrodynamic diameters ranged from 158 nm (nanoemulsion), 360 nm (one polymeric layer) to 398 nm (two polymeric layers). NCs presented, during the in vitro release, the release of eugenol following a first order process. In addition to being in the region of stability (zeta potential ca. |30| mV), the capsules showed good adhesion to the nectarine surface. In relation to brown rot, the eugenol NCs with chitosan proved to be the best formulation compared to nanoemulsion and NCs with two polymeric layers for its control, increasing the probability that the fruits remain without symptoms, even after 7 days. Therefore, this study demonstrated that chitosan NCs containing eugenol could be an alternative to preserve fruit for longer periods in post-harvest.

https://doi.org/10.21577/0103-5053.20230018

PCR identification of lactic acid bacteria populations in corn silage inoculated with lyophilised or activated Lactobacillus buchneri
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Santos, Ana Paula Maia dos

; Santos, Edson Mauro

; Silva de Oliveira, Juliana

; Garcia Leal de Araújo, Gherman

; de Moura Zanine, Anderson

; Araújo Pinho, Ricardo Martins

; Costa do Nascimento, Thiago Vinicius

; Fernandes Perazzo, Alexandre

; Ferreira, Daniele de Jesus

; da Silva Macedo, Alberto Jefferson

; de Sousa Santos, Francisco Naysson

Revista de la Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo

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jun 2023, Volume 55 N. 1 Pages 115 - 125

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Este estudio tuvo como objetivo evaluar el efecto de la inoculación con L. buchneri liofilizado y/o activado sobre las poblaciones de bacterias de ácido láctico en ensilajes de maíz. Los tratamientos experimentales consistieron en ensilaje de maíz sin aditivo y ensilaje con los inoculantes L. buchneri 1 × 10 5 ufc/g, aplicados según las recomendaciones del fabricante (1 g/tonelada de forraje) en las formas de inoculante liofilizado e inoculante preactivado. Se identificaron aislados purificados de ensilajes de maíz con y sin inocu lación, y el 93% de los aislados correspondieron a bacterias de ácido láctico de la especie L. plantarum. Entre los aislados no se detectaron bacterias de la especie L. buchneri. La aplicación de L. buchneri liofilizado o activado en la masa ensilada promueve un aumento considerable de la población de bacterias de ácido lácticas en los ensilajes de maíz durante todo el período de fermentación.

Abstract This study aimed to evaluate the effect of inoculation with lyophilised and/or activated Lactobacillus buchneri on lactic acid bacteria populations in corn silage. Experimental treatments consisted of corn silage without additives or silage with the inoculants of L. buchneri (1 x 10 5 cfu/g) applied according to the manufacturer’s recommendations (1 g/tonne fodder) in the forms of the lyophilised inoculant and pre-activated inoculant. Purified isolates from corn silage with and without the inoculant were identified, and 93% of the isolates corresponded to the lactic acid bacteria of the species Lactobacillus plantarum. Among the isolates, no bacteria of the species L. buchneri were detected. The application of lyophilised or activated L. buchneri improved the microbiological profile and reduced ethanol production in corn silage, even without being identified among the isolates captured 70 days after ensilage.

Soybean performance in succession to the intercropping of corn with marandu grass and pigeonpea in an integrated agricultural production system
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Pereira, Roseana Ramos

; Garcia, Izabela Militão

; Modesto, Viviane Cristina

; Sekiya, Bianca Midori Souza

; Soares, Deyvison de Asevedo

; Andreotti, Marcelo

Revista Ceres

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jun 2023, Volume 70 N. 3 Pages 72 - 80

Abstract: En | Text: En | PDF: En

ABSTRACT Integrated agricultural production systems have been improved with the introduction of Urochloa grasses aiming at obtaining straw for a no-tillage system (NTS) and the increase of legumes, which promote physicochemical improvements in soils and guarantee intensification and sustainability of production. This study aimed to evaluate the agronomic traits and yield of soybean in succession to the intercropping of corn with marandu grass and/or pigeonpea in five populations of plants harvested for silage production. The experiment was carried out in the Cerrado region of Selvíria, MS, Brazil, during the 2016/17 and 2017/18 agricultural years, in an Oxisol. The experimental design consisted of randomized blocks, with four replications. The treatments were arranged in a 2×5 factorial scheme, consisting of off-season corn intercropped with pigeonpea (Cajanus cajan (L.) Millsp.) and off-season corn intercropped with marandu grass (Urochloa brizantha) and pigeonpea, in addition to five sowing densities (0, 6, 12, 18, and 24 seeds m-1). Soybean was planted in succession aiming to evaluate the effect of remaining straw on crop performance. Soybean grown in succession to the triple intercropping of corn with marandu and pigeon pea shows increased productivity.

https://doi.org/10.1590/0034-737x202370030008

[SciELO Preprints] - Passifloraceae s.s. from the São Luís Island, Maranhão State, Brazil
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Garcia, Amanda Letícia Silva, Eduardo Oliveira Ferreira, Alessandro Wagner Coelho Marinho, Lucas Cardoso

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Publication date: 2023-05-16

Abstract: En Pt

The São Luís Island, Maranhão State, is an ecotonal region, a mix of humid forest, cocais forest, dune vegetation, restingas and mangroves. Unlike the interior of the State, their coastal areas have a gap in knowledge of the Passifloraceae. Here, through field expeditions and herbaria visit, we provide taxonomic treatment, identification key and comments on the Passifloraceae of the São Luís Island. Ten species were recorded: Passiflora auriculata Kunth, P. edulis Sims, P. foetida L., P. glandulosa Cav., P. laurifolia L., P. misera Kunth, P. nitida Kunth, P. picturata Ker Gawl., P. subrotunda Mast., and P. vespertilio L. Passiflora picturata and P. misera were recorded for the first time on São Luís Island.

A Ilha de São Luís, Estado do Maranhão, configura-se como uma região ecotonal, um misto de floresta úmida, mata-de-cocais, vegetação de dunas, restingas e manguezais. Diferentemente do interior do Estado do Maranhão, suas zonas costeiras possuem uma lacuna sobre o conhecimento da família Passifloraceae. Aqui, por meio de expedições de campo e consulta a herbários, nós fornecemos o tratamento taxonômico, chave de identificação e comentários sobre as Passifloraceae da Ilha de São Luís. Foram registradas dez espécies: Passiflora auriculata Kunth, P. edulis Sims, P. foetida L., P. glandulosa Cav., P. laurifolia L., P. misera Kunth, P. nitida Kunth, P. picturata Ker Gawl., P. subrotunda Mast. e P. vespertilio L. As espécies P. picturata e P. misera foram registradas pela primeira vez na Ilha de São Luís.

https://doi.org/10.1590/2236-8906-56/2022

10.

Chemical composition, antioxidant activity and cytotoxicity of flower extracts from Magnolia grandiflora L. found in southeast Mexico
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Rivas-Garcia, Tomas

; Alejandre-Rosas, Jorge Alberto

; Ramos-Ligonio, Ángel

; Castillo-Morales, Marisol

; Reyes-Pérez, Juan José

; Esquivel-Valenzuela, Berenice

Biotecnia

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apr 2023, Volume 25 N. 1 Pages 5 - 13

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Algunos estudios afirman que diferentes estructuras de M. grandiflora contienen componentes bioactivos. No obstante, los estudios fitoquímicos indican que los aceites esenciales extraídos son químicamente diferentes y notablemente variables en sus composiciones cualitativas y cuantitativas. Es necesario realizar más estudios sobre M. grandiflora cultivada en México. Por ello, el objetivo de esta investigación fue caracterizar a) la composición química, b) la actividad antioxidante y c) el efecto citotóxico de dos extractos de flores de M. grandiflora. La composición química se evaluó mediante una prueba fitoquímica preliminar seguida de cromatografía en capa fina y análisis espectrofotométrico mediante UV-Vis y FTIR. La actividad antioxidante de los extractos de flores se midió por el método de la actividad reductora de radicales libres (ABTS) y el método de radicales estables de 2,2-difenil-1-picril-hidrazilo (DPPH) y la citotoxicidad por un bioensayo de Artemia salina. Los extractos acuoso y etílico de flores mostraron la presencia de cromóforos orgánicos como los flavonoides. Ambos extractos (etílico y acuoso) demostraron actividad antioxidante tanto por ABTS (459.6 ± 8.5 y 274.2 ± 5.7 µmol TE/g, respectivamente) y DPPH (3210.4 ± 2.5 y 219.7 ± 0.9 µmol TE/g, respectivamente); y actividad no citotóxica (CL50, µg/mL) (1285 ± 14 y 1116 ± 15, respectivamente). Los extractos acuosos y etílicos de las flores de M. grandiflora encontradas en el sureste de México son una fuente promisoria de compuestos químicos con actividad biológica atribuida por los resultados presentados.

Abstract Some reports state that different structures of M. grandiflora contain bioactive components. Nonetheless, phytochemical studies reported that the extracted essential oils are chemically different and remarkably variable in their qualitative and quantitative compositions. Further studies on the Mexican M. grandiflora needs to be done. Thus, the research aimed to characterize a) the chemical composition, b) the antioxidant activity and c) cytotoxicity effect of two M. grandiflora flower extracts. The chemical composition was evaluated by phytochemical test followed by thin layer chromatography, UV-Vis and FTIR spectrophotometer analysis. The antioxidant activity of the flower extracts was measured by the free radical-scavenging activity (ABTS) and the stable radical of 2,2-diphenyl-1-picryl-hydrazyl (DPPH) methods, and the cytotoxicity by an Artemia salina bioassay. Water and ethyl flower extracts showed the presence of organic chromophores such as flavonoids. Both extracts (ethyl and water) demonstrated antioxidant activity by both ABTS (459.6 ± 8.5 and 274.2 ± 5.7 µmol TE/g, respectively) and DPPH (3210.4 ± 2.5 and 219.7 ± 0.9 µmol TE/g, respectively) methodologies, and non-cytotoxic activity (LC50, µg/mL) (1285 ± 14 and 1116 ± 15, respectively). The water and ethyl extracts of M. grandiflora flowers found in southeast Mexico are a promissory source of chemical compounds with attributed biological activity according to the presented results.

https://doi.org/10.18633/biotecnia.v25i1.1680

11.

Impact of fuel quantity on luminescence properties of Sr3Al2O6:Eu by combustion synthesis
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Barbosa, W. T.

; Álvarez-Docio, C. M.

; Garcia-Carrodeguas, R.

; Fook, M. V. L.

; Rodríguez, M. A.

; Rojas-Hernandez, R. E.

Cerâmica

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jan 2023, Volume 69 N. 389 Pages 17 - 22

Abstract: En | Text: En | PDF: En

Abstract The photoluminescent behavior of Eu-doped Sr3Al2O6 obtained by highly efficient solution combustion synthesis is reported. In order to understand the influence of the fuel on the synthesis, the stoichiometric quantity and an excess of fuel were evaluated. By adjusting the amount of fuel, different luminescence responses were obtained, allowing europium cations incorporation into the Sr3Al2O6 lattice to serve as effective luminescence activators in such a short time during the rapid combustion synthesis process. The higher amount of fuel in the presence of the oxidizing agent produced Sr3Al2O6:Eu particles with higher phosphorescence brightness, owing to the increase of the reduction process from Eu3+ to Eu2+. The synthesized phosphor showed an intense band emission centered at 515 nm and could be excited over a broad spectral range in the UV-visible region. Particles having nanostructured flake-type morphology were obtained, which was considered a micro-nanofunctional candidate for practical applications.

https://doi.org/10.1590/0366-69132023693893379

12.

Early Six-Minute Walk Test May Predict Midterm Outcomes Following Coronary Artery Bypass Grafting
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Pauletti, Hayanne O.

; Gomes, Walter José

; Rocco, Isadora S.

; Viceconte, Marcela

; Garcia, Bruna Caroline Matos

; Marcondi, Natasha O.

; Bublitz, Caroline B.

; Costa, Ariele dos Santos

; Paiva, Thâmara Pequeno de

; Spina, Giovanna Domingues

; Begot, Isis

; Silva, Célia Camelo

; Moreira, Rita Simone L.

; Branco, João Nelson Rodrigues

; Vargas, Guilherme Flora

; Hossne Jr., Nelson A.

; Arena, Ross

; Guizilini, Solange

Brazilian Journal of Cardiovascular Surgery

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2023, Volume 38 N. 4 elocation e20220459

Abstract: En | Text: En | PDF: En

ABSTRACT Objective: This study aims to investigate the ability of the six-minute walk distance (6MWD) as a prognostic marker for midterm clinical outcomes three months after coronary artery bypass grafting (CABG), to identify possible predictors of fall in 6MWD in the early postoperative period, and to establish the percentage fall in early postoperative 6MWD, considering the preoperative baseline as 100%. Methods: A prospective cohort of patients undergoing elective CABG were included. The percentage fall in 6MWD was assessed by the difference between preoperative and postoperative day (POD) five. Clinical outcomes were evaluated three months after hospital discharge. Results: There was a significant decrease in 6MWD on POD5 compared with preoperative baseline values (percentage fall of 32.5±16.5%, P<0.0001). Linear regression analysis showed an independent association of the percentage fall of 6MWD with cardiopulmonary bypass (CPB) and preoperative inspiratory muscle strength. Receiver operating characteristic curve analysis revealed that the best cutoff value of percentage fall in 6MWD to predict poorer clinical outcomes at three months was 34.6% (area under the curve = 0.82, sensitivity = 78.95%, specificity = 76.19%, P=0.0001). Conclusion: This study indicates that a cutoff value of 34.6% in percentage fall of 6MWD on POD5 was able to predict poorer clinical outcomes at three months of follow-up after CABG. Use of CPB and preoperative inspiratory muscle strength were independent predictors of percentage fall of 6MWD in the postoperative period. These findings further support the clinical application of 6MWD and propose an inpatient preventive strategy to guide clinical management over time.

https://doi.org/10.21470/1678-9741-2022-0459

13.

Propiedades antioxidantes y antiinflamatorias de huauzontle (Chenopodium berlandieri subsp. nuttalliae, Chenopodiaceae) fermentado por Lactiplantibacillus plantarum Lp22
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Santiago-López, Lourdes

; Garcia, Hugo S.

; González-Córdova, Aarón F.

; Vallejo-Cordoba, Belinda

; Hernandez-Mendoza, Adrian

Acta botánica mexicana

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2023, N. 130 elocation e2161

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract: Background and Aims: Huauzontle (Chenopodium berlandieri subsp. nuttalliae) is a pseudocereal native to Mexico and is part of its culinary tradition. This pseudocereal is recognized for its high nutritional value and components that are associated with beneficial health effects. However, there is currently no literature on the impact that the fermentation process would have on its bioactive properties. Therefore, the aim of this research was to evaluate the effect of fermentation by Lactiplantibacillus plantarum Lp22 in huauzontle seeds suspended in water on their antioxidant and anti-inflammatory properties in vitro. Methods: Huauzontle seeds were suspended in water (0.3%, w/v), inoculated with L. plantarum Lp22 (1%, v/v) at 108 CFU/ml, and allowed to ferment for 24 h at 37 °C. Then, seeds were recovered by filtration, grounded and aqueous extract was obtained. The antioxidant activity of the extracts was determined by the ABTS and ORAC methods. Moreover, the inhibition of the enzymes lipoxygenase (LOX) and trypsin was evaluated and the total phenolic compounds in methanolic extract (Folin-Ciocalteu method) were also determined. Key results: The antioxidant activity represented as (M of Trolox equivalents was 812.50 and 927.50 by ABTS and 1384.32 and 3391.11 for ORAC, for unfermented and fermented huauzontle, respectively (p<0.05). The inhibition of LOX activity was 40.06% and 37.24% for unfermented and fermented huauzontle, respectively, while for trypsin it was 12.53% and 14.38% (p>0.05). Besides, total phenol content was 7.49 and 20.24 (g/ml (p<0.05), respectively. Conclusions: Our findings evidence that fermentation of huauzontle improves the antioxidant and anti-inflammatory properties, which may be associated with increased phenol content. Therefore, its consumption could enhance the antioxidant and immune system of the human organism.

Resumen: Antecedentes y Objetivos: El huauzontle (Chenopodium berlandieri subsp. nuttalliae) es un pseudocereal nativo de México y forma parte de su tradición culinaria. Este pseudocereal es reconocido por su alto valor nutricional y componentes que se asocian a efectos benéficos a la salud. Sin embargo, al momento no se cuenta con literatura sobre el impacto que tendría el proceso de fermentación sobre sus propiedades bioactivas. Por ello, el objetivo de la presente investigación fue evaluar el efecto de la fermentación por Lactiplantibacillus plantarum Lp22, en semillas de huauzontle suspendidas en agua, sobre sus propiedades antioxidantes y antiinflamatorias in vitro. Métodos: Semillas de huauzontle se suspendieron en agua (0.3% p/v), se inocularon con L. plantarum Lp22 (1% v/v) a una concentración de 108 UFC/ml y se fermentaron por 24 h a 37 °C. Finalmente, las semillas fueron recuperadas por filtración, se molieron y del extracto acuoso se determinó la actividad antioxidante por los métodos de ABTS y ORAC. Además, se evaluó la inhibición de las enzimas lipooxigenasa (LOX) y tripsina y se determinó la concentración de fenoles totales (método de Folin-Ciocalteu) en extractos metanólicos. Resultados clave: La actividad antioxidante representada como (M de equivalentes Trolox fue de 812.50 y 927.50 por ABTS, y 1384.32 y 3391.11, para ORAC, para huauzontle no fermentado y fermentado, respectivamente (p<0.05). La inhibición de la actividad de LOX fue de 40.06% y 37.24%, y para tripsina fue de 12.53% y 14.38% (p>0.05), para no fermentado y fermentado, respectivamente, mientras que el contenido de fenoles totales fue de 7.49 y 20.24 (g/ml (p<0.05), respectivamente. Conclusiones: Estos hallazgos indican que la fermentación del huauzontle mejora las propiedades antioxidantes y antiinflamatorias, y que pueden estar asociados con el incremento en el contenido de fenoles, por lo que su consumo podría fortalecer el mecanismo antioxidante y sistema inmune del organismo humano.

https://doi.org/10.21829/abm130.2023.2161

14.

Collaboration for success: the Global Initiative for Childhood Cancer in Latin America
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Vásquez, Liliana

; Fuentes-Alabi, Soad

; Benitez-Majano, Sara

; Ribeiro, Karina Braga

; Abraham, Monnie

; Agulnik, Asya

; Baker, Justin N.

; Blanco, Daniel Bastardo

; Caniza, Miguela A.

; Cardenas-Aguirre, Adolfo

; Salaverria, Carmen

; Sullivan, Courtney E.

; Damasco-Avila, Erika

; García-Quintero, Ximena

; Loggetto, Patricia

; McNeil, Michael J.

; Luna-Fineman, Sandra

; Rossell, Nuria

; Garcia de Lima, Regina Aparecida

; de Mendonca, Regina Holanda

; Trigoso, Viviana

; Segovia, Lorena

; Vasquez, Roberto

; Moreno, Florencia

; Friedrich, Paola

; Luciani, Silvana

; Lam, Catherine

; Metzger, Monika L.

; Rodríguez-Galindo, Carlos

; Maza, Mauricio

Revista Panamericana de Salud Pública

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2023, Volume 47 elocation e144

Abstract: Pt Es En | Text: Pt Es En | PDF: Pt | PDF: Es | PDF: En

RESUMO A Iniciativa Global para o Câncer Infantil tem como objetivo aumentar a taxa de cura de crianças com câncer no mundo todo, melhorando o acesso a cuidados e a qualidade da assistência médica. A Organização Pan-Americana da Saúde (OPAS), o St. Jude Children's Research Hospital (St. Jude) e colaboradores uniram esforços para melhorar o desfecho de crianças com câncer na América Latina e no Caribe (ALC) no âmbito do marco CureAll. Neste artigo, descrevemos o processo de desenvolvimento de recursos regionais com o objetivo de acelerar a implementação da Iniciativa na ALC. Em março de 2021, a OPAS formou grupos de trabalho regionais para desenvolver projetos centrais alinhados com os pilares e facilitadores do CureAll. A partir das reuniões de diálogo regionais, foram criados sete grupos de trabalho: detecção precoce, enfermagem, atenção psicossocial, nutrição, cuidados de suporte, abandono do tratamento e cuidados paliativos. A OPAS organizou reuniões virtuais regulares sob a orientação e o apoio dos programas regionais e transversais do St. Jude e de mentores internacionais. Entre abril e dezembro de 2021, 202 especialistas multidisciplinares participaram de 43 reuniões virtuais para promover o diálogo entre as partes interessadas a fim de melhorar os desfechos do câncer infantil. Foram produzidos 14 materiais técnicos: quatro panoramas regionais, quatro documentos técnicos, dois cursos virtuais, um conjunto de perfis epidemiológicos nacionais, uma série de conteúdo educacional para pais e cuidadores e duas campanhas de comunicação. O diálogo e o compromisso contínuos da OPAS, do St. Jude, dos comitês de trabalho da ALC e dos colaboradores internacionais são bases essenciais para acelerar com sucesso a implementação da Iniciativa Global para o Câncer Infantil. Isso é possível por meio do desenvolvimento de materiais de relevância regional e mundial. São necessárias mais pesquisas e avaliações para determinar o impacto dessas estratégias e recursos nos resultados do câncer infantil na ALC e em outras regiões.

RESUMEN La Iniciativa Mundial contra el Cáncer Infantil tiene como objetivo aumentar a nivel mundial la tasa de curación del cáncer infantil mediante la mejora del acceso a la atención de salud y de su calidad. La Organización Panamericana de la Salud (OPS), el St. Jude Children’s Research Hospital y los colaboradores han aunado esfuerzos para mejorar los resultados en la población infantil con cáncer en América Latina y el Caribe valiéndose del marco CureAll. En este artículo describimos el proceso de elaboración de recursos regionales destinados a acelerar la aplicación de la Iniciativa Mundial en América Latina y el Caribe. En marzo del 2021, la OPS formó grupos de trabajo regionales para elaborar proyectos básicos que estuvieran en consonancia con los pilares y los elementos facilitadores del CureAll. De los diálogos regionales surgieron siete grupos de trabajo: detección temprana, enfermería, aspectos psicosociales, nutrición, tratamientos de apoyo, abandono del tratamiento y cuidados paliativos. La OPS organizó con regularidad reuniones virtuales en las que se contó con la tutoría y el apoyo de programas regionales o transversales del St. Jude Children’s Research Hospital y de mentores internacionales. Entre abril y diciembre del 2021 hubo 43 reuniones virtuales a las que asistieron 202 expertos multidisciplinarios, con el objetivo de promover el diálogo entre las partes interesadas para mejorar los resultados en materia de cáncer infantil. Se elaboraron catorce productos técnicos: cuatro panoramas regionales, cuatro documentos técnicos, dos cursos virtuales, un conjunto de perfiles epidemiológicos de países, una serie con contenidos educativos para padres y cuidadores y dos campañas de comunicación. El diálogo y el compromiso constantes de la OPS, el St. Jude Children’s Research Hospital, los comités de trabajo de América Latina y el Caribe y los colaboradores internacionales son las bases fundamentales para conseguir que se acelere la aplicación de la Iniciativa Mundial. Esto se puede lograr mediante la elaboración de materiales que resulten pertinentes a nivel regional y mundial. Son necesarias más investigaciones y evaluaciones para determinar el impacto que tienen estas estrategias y recursos en los resultados que se obtienen en el cáncer infantil en América Latina y el Caribe y en otras subregiones.

ABSTRACT The Global Initiative for Childhood Cancer (GICC) aims to increase the cure rate for children with cancer globally by improving healthcare access and quality. The Pan American Health Organization (PAHO), St. Jude Children’s Research Hospital (St. Jude), and collaborators have joined efforts to improve outcomes of children with cancer in Latin America and the Caribbean (LAC) using the CureAll framework. In this article, we describe the process of developing regional resources aimed at accelerating the GICC implementation in LAC. In March 2021, PAHO formed regional working groups to develop core projects aligned with CureAll pillars and enablers. Seven working groups emerged from regional dialogues: early detection, nursing, psychosocial, nutrition, supportive care, treatment abandonment, and palliative care. PAHO arranged regular online meetings under the mentorship and support of St. Jude regional/transversal programs and international mentors. Between April and December 2021, 202 multidisciplinary experts attended 43 online meetings to promote the dialogue between stakeholders to improve childhood cancer outcomes. Fourteen technical outputs were produced: four regional snapshots, four technical documents, two virtual courses, one set of epidemiological country profiles, one educational content series for parents/caregivers, and two communication campaigns. The ongoing dialogue and commitment of PAHO, St. Jude, LAC working committees, and international collaborators are essential foundations to successfully accelerate GICC implementation. This is achievable through the development of materials of regional and global relevance. Further research and evaluation are needed to determine the impact of these strategies and resources on childhood cancer outcomes in LAC and other regions.

https://doi.org/10.26633/rpsp.2023.144

15.

Electrical Conductivity and Nitrogen:Potassium Ratios from Nutrigation in the Quality of Zucchini Fruits
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Pacheco, Adriano Bicioni

; Duarte, Sergio Nascimento

; Charles, Thaís da Silva

; Nascimento, Jéssica Garcia

; Simões, Ana Laura Toledo

; Campos Júnior, José Eustáquio

; Almeida, Alex Nunes de

; Ricardo, Hugo de Carvalho

; Lopes, Tárcio Rocha

; Jesus, Fernanda Lamede Ferreira de

; Lisboa, Cristiane Fernandes

Brazilian Archives of Biology and Technology

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2023, Volume 66 elocation e23210845

Abstract: En | Text: En | PDF: En

Abstract The demands of high-quality zucchini fruits by the consumer market make studies regarding the improvement of cultivation systems, such as plant nutrition and salinity tolerance. Considering the demands of the consumer market for the high quality of zucchini fruits, it is necessary studies regarding the improvement of production systems for this improvement, such as nutrition and salinity tolerance to plant. Thus, this study aimed to evaluate the zucchini fruits quality characteristics, grown in soilless, under N:K ratios, and levels of electrical conductivity of the nutrient solution. The experiment was conducted in a protected environment in pots with 8.2 dm3 of volumetric capacity filled with commercial substrate. The trial design adopted was randomized blocks, with three replicates, in a 5x3 factorial scheme, with five levels of electrical conductivity (1.0; 2.0; 3.0; 4.0; and 5.0 dS m-1) and three N:K ratios (2:1; 1:1; and 1:2), both in the nutrient solution. The application of the nutrient solution was by fertigation through drip irrigation. Throughout the experiment, variables related to fruit quality and total plant biomass production were analyzed. The increase of the electrical conductivity in the nutrient solution provided an increase in the values of fruits organoleptic properties. Regarding the nutrient solution N:K ratios, the 1:2 ratio provided larger fruits and a higher percentage of water.

https://doi.org/10.1590/1678-4324-2023210845

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