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au:GOMES, JESSICA S.

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COMPARANDO A EFICIÊNCIA ANALÍTICA DAS TÉCNICAS FTIR, UV-VIS, CLAE-DAD E ESI(+)MS NO ESTUDO DE CORANTES ALIMENTARES
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Lyrio, Marcos V. V.

; Debona, Danieli G.

; Conceição, Nathália dos S.

; Gomes, Francisco M.

; Pereira, Alan R.

; Frizzera, Henrique A.

; Reis, Jessica L.

; Grecco, Taila T.

; Pires, Bruna C.

; Lacerda Jr., Valdemar

; Castro, Eustaquio V. R.

; Isidoro, Marsele M.

; Filgueiras, Paulo R.

; Romão, Wanderson

Química Nova

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2023, Volume 46 N. 5 Pages 435 - 449

Abstract: Pt | Text: Pt | PDF: Pt

Food additives are chemical substances added to improve organoleptic characteristics, in this context, there are several synthetic dyes, such as sunset yellow, tartrazine, erioglaucine, allura red, indigo carmine, and new coccine, that can also be identified through different analytical techniques such as infrared spectroscopy, UV-Vis spectrophotometry, high performance liquid chromatography with diode array detector and mass spectrometry. Therefore, the objective of this work was to compare methods developed for different analytical techniques, in order to identify the one that presents greater efficiency in certain applications. For this, the patterns of the dyes were analyzed, as well as their mixtures and foods that had them. As a result, the CLAE-DAD presents excellent selectivity and linearity of the calibration curves, but it presents higher LD and LQ values when compared to the UV-Vis, in turn the infrared provides information regarding functional groups and bonds. Mass spectrometry showed great capabilities in identifying the molecular formula and better selectivity in the absence of the erioglaucine dye. It is not feasible to indicate the best or worst analytical approach, but it is possible to point out which one is more suitable for each application. Regarding the quantification capacity, chromatography and UV-Vis are good alternatives, but in mixtures containing more than one dye, CLAE-DAD is more suitable due to the better selectivity. Regarding the identification capacity, mass spectrometry, especially FT-ICR MS, is the most appropriate technique to propose molecular formulas due to its high resolution and mass accuracy. The advantage of FTIR was the easier sample preparation, being able to analyze the dyes in the solid state.

https://doi.org/10.21577/0100-4042.20230037

An integrative study of the invasive jumping-snail Ovachlamys fulgens (Gastropoda, Helicarionidae) in Rio de Janeiro and its fast spreading in Southeastern and Southern Brazil
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MARCHI, CAROLINA R.

; CORRÊA-ANTÔNIO, JESSICA

; RODRIGUES, PAULO SÉRGIO

; FERNANDEZ, MONICA A.

; THIENGO, SILVANA C.

; BARBOSA, HELENE S.

; GOMES, SUZETE R.

Anais da Academia Brasileira de Ciências

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2021, Volume 93 N. 2 elocation e20190138

Abstract: En | Text: En | PDF: En

Abstract The Japanese invasive jumping snail Ovachlamys fulgens is a pest of ornamental plants and an intermediate host of a nematode that causes eosinophilic meningitis. We expand its distribution to eight municipalities from Rio de Janeiro State, and one locality from the Paraná State, and generated for the first time partial sequences of the cytochrome c oxidase subunit I (COI) gene for Brazilian populations. External morphology, reproductive system, shell, radula, and jaw were also analyzed and described. Twenty-one lots were collected from Rio de Janeiro, Niterói, Magé, Miguel Pereira, Petrópolis, Teresópolis, Nova Friburgo, Bom Jardim and Paraty, in Rio de Janeiro State, and from Foz do Iguaçu, Paraná State. External morphology, shell and reproductive system were typical of O. fulgens, with some peculiarities found in the shell and radula. A single haplotype was found, which was 100% similar to sequences of COI available in GenBank for specimens from Japan and Argentina. The species seems to be adapted to many habitats and be rapidly expanding its distribution in Southeastern and Southern Brazil, and other South America countries. We highlight the importance of monitoring O. fulgens, considering its potential to compete with native mollusks, attack several plants, and be a transmitter of diseases.

https://doi.org/10.1590/0001-3765202120190138 636 downloads

One-Step Isolation of Monoterpene Indole Alkaloids from Psychotria leiocarpa Leaves and Their Antiviral Activity on Dengue Virus Type-2
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Costa, Jéssica O.

; Barboza, Rodolfo S.

; Valente, Ligia M. M.

; Wolff, Thiago

; Gomes, Mário

; Gallo, Blanca

; Berrueta, Luis A.

; Guimarães-Andrade, Iris P.

; Gavino-Leopoldino, Daniel

; Assunção-Miranda, Iranaia

Journal of the Brazilian Chemical Society

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oct 2020, Volume 31 N. 10 Pages 2104 - 2113

Abstract: En | Text: En | PDF: En

The leaf MeOH extract of Psychotria leiocarpa (Rubiaceae) showed in vitro non-cytotoxic and anti-dengue virus serotype 2 (DENV2) activity in human hepatocarcinoma cell lineage (HepG2). A one-step and cost-effective reversed-phase solid-phase extraction method based on high-performance liquid chromatography (HPLC) parameters allowed the isolation, directly from this bioactive extract, of the monoterpene indole alkaloids: N-glucopyranosyl vincosamide (1), vincosamide (2) and strictosidinic acid (3). The chemical structures were characterized based on 1D and 2D nuclear magnetic resonance (NMR), UV and high-resolution mass spectra (HRMS). The methodology has also allowed yielding a polyphenolic-rich fraction that was analyzed by high-performance liquid chromatography coupled to diode array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI-MS/MS) revealing two flavonol triglycosides (4, 5) and three caffeoylquinic acid isomers (6-8). Compound 3 is reported for the first time in P. leiocarpa and all the phenolic compounds (4-8) are described for the first time in the genus Psychotria. Compounds 1-3 showed to be non-cytotoxic and anti-dengue active towards DENV2, highlighting vincosamide (2).

https://doi.org/10.21577/0103-5053.20200111 327 downloads

Leachate and vinasse used in a biological process combined with Fenton’s reaction: a green method for treatment of textile effluents
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BATTISTI, ANDRE C.

; SANTOS, HYALLE M.M.F. DOS

; ALBERTON, JÉSSICA S.

; GOMES, LETÍCIA M.F.

; RAUPP, LUCAS K.

; GRACIANO, MARIANE R.

; OLIVEIRA, PÂMELA E. DE

; SILVA JUNIOR, JULIO L. DA

; DIAS, RAFAEL CARLOS E.

Anais da Academia Brasileira de Ciências

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2019, Volume 91 N. 4 elocation e20181156

Abstract: En | Text: En | PDF: En

Abstract: The production of textiles generates a large amount of liquid residues considered to be environmentally problematic and which, if discarded without due treatment, may cause eutrophication of water sources, the intoxication of living beings, and the blocking of light penetration. Some methods have been applied for the treatment of this type of waste, with the prominence of biological and physicochemical techniques. The combination of two or more techniques enhances the treatment’s efficiency. This work investigated the use of bacteria derived from the leachate produced by the textile industry itself, fed with vinasse, a by-product of the alcohol industry, with subsequent application of Fenton’s reaction. The biological method, optimized in terms of aeration and quantity of nutrients for the bacteria, combined with the physicochemical method optimized in terms of the amount of reagents, was efficient for the discoloration of the effluent in approximately 100 %, for toxicity reduction, for the degradation of organic matter in 80 %, and with acceptable acidity for disposal after only eight days of treatment. The method was considered of great efficiency and low application cost, without the production of hazardous waste.

https://doi.org/10.1590/0001-3765201920181156 825 downloads

Bone marrow stem cell applied in the canine veterinary clinics
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Gomes, Ingrid S.

; Oliveira, Vanessa C. de

; Pinheiro, Alessandra O.

; C.S.Roballo, Kelly

; Araujo, Giuliano S.M. de

; Veronezi, Jessica C.

; Martins, Daniele S.

; Ambrósio, Carlos Eduardo

Pesquisa Veterinária Brasileira

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oct 2017, Volume 37 N. 10 Pages 1139 - 1145

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: A terapia celular representa uma antiga prática terapêutica iniciada com a transfusão de sangue total em diferentes situações clínicas. Atualmente há um avanço no estudo da terapia com células-tronco mesenquimais por conta de sua funcionalidade na regeneração de tecidos, o que promove uma crescente atenção do meio científico. A medula óssea é uma das principais fontes de células-tronco multipotentes, no qual se encontram as células-tronco hematopoiéticas, responsável pela renovação dos componentes celulares do sangue, e as células-tronco mesenquimais que auxiliam na regeneração de tecidos. Essas células têm um forte potencial para o tratamento de diversas enfermidades, uma vez que possuem como principais características alta plasticidade, capacidade de auto renovação e imunomodulação. Apesar de haver muitos trabalhos que apresentam bons resultados com a utilização da terapia celular como forma de tratamento para diversas enfermidades, alguns estudos ainda demonstram resultados inconclusivos ou não satisfatórios, por isso, objetivou-se com este trabalho revisar a aplicação das células-tronco derivadas da medula óssea no modelo canino uma vez que é necessário melhorias sobre o conhecimento da técnica para que possibilite a sua aplicabilidade com segurança e eficácia.

ABSTRACT: Cell therapy represents an old therapeutic practice initiated with the transfusion of whole blood in different clinical situations. There is now a breakthrough in the study of multipotent stem cell therapy because of its functionality in regeneration of tissues, which promotes attention of the scientific community. Bone marrow is one of the main sources of multipotent stem cells, composed by hematopoietic stem cells responsible for the renewal of the cellular components of the blood, and mesenchymal stem cells that aid in the regeneration of tissues. These cells have a strong potential for the treatment of several diseases, due their main characteristics such as high plasticity, capacity for self-renewal and immunomodulation. Although, there are many studies that show good results with the use of cell therapy as a form of treatment for several diseases, some studies still show inconclusive or unsatisfactory results. Therefore, the objective of this study was to review the application of bone marrow stem cells in the canine model since improvements on the knowledge of the technique are necessary to enable its applicability with safety and efficacy.

https://doi.org/10.1590/s0100-736x2017001000016 3031 downloads

Chemical composition and antibacterial activity of essential oil and leaf extracts of Zanthoxylum caribaeum Lam. against serotypes of Salmonella
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SOUZA, Juliete Gomes de Lara de

; TOLEDO, Adrieli Gorlin

; SANTANA, Camila Beatriz

; SANTOS, Camila Vogt dos

; MALLMANN, Ana Paula

; SILVA, Jéssica Patrícia Borges da

; PINTO, Fabiana Gisele da Silva

Revista Brasileira de Saúde e Produção Animal

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jul 2017, Volume 18 N. 3 Pages 446 - 453

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivou-se com este estudo identificar e quantificar os constituintes do óleo essencial de Zanthoxylum caribaeum e avaliar o potencial antimicrobiano de diferentes extratos vegetais e do óleo essencial desta planta frente a diferentes sorotipos de Salmonella enterica, de maior ocorrência e isoladas na região oeste do Paraná, Brasil. A extração do óleo essencial foi realizada utilizando aparelho Clevenger e a composição química foi determinada por Cromatografia Gasosa acoplada a Espectrometria de Massas (CG-EM). Os extratos foram obtidos através de diferentes solventes (etanol, metanol, hexano, acetona, diclorometano, acetato de etila e água destilada). A atividade antibacteriana foi realizada segundo a técnica de microdiluição em caldo. As análises de CG-EM resultaram na identificação de 15 constituintes principais, todos terpenos, representando 63,88 % do total de óleo essencial. Os compostos majoritários identificados foram Germacreno-D (20,77 %), a-Panasinseno (14,40 %) e b-Selineno (11,68 %). O óleo essencial apresentou atividade antibacteriana frente a 5 sorotipos de Salmonella enterica, com CIM e CBM variando de 7000 a 437 µg.mL-1. Já os extratos variam a CIM e CBM de 200 a 25 mg.mL-1, sendo eficazes para a maioria dos sorotipos de S. enterica, com exceção do extrato aquoso. Os resultados sugerem que o óleo essencial e os extratos das folhas de Z. caribaeum representam uma alternativa para o controle de S. enterica, no setor avícola, refletindo uma nova perspectiva para estudos com produtos naturais.

SUMMARY The present study aimed to identify and quantify the components of the essential oil of Zanthoxylum caribaeum and to evaluate the antimicrobial potential of different plant extracts and the essential oil of this plant against different serotypes of Salmonella enterica, which is of greater occurrence and isolated in the western region of Paraná, Brazil. The extraction of the essential oil was performed using Clevenger apparatus and the chemical composition was determined by Gas Chromatography coupled to Mass Spectrometry (GC-MS). The extracts were obtained through different solvents (ethanol, methanol, hexane, acetone, dichloromethane, ethyl acetate and distilled water). The antibacterial activity was carried out according to the broth microdilution technique. The GC-MS analysis resulted in the identification of 15 main components, all terpenes, representing 63.88% of the total essential oil. The major compounds identified were Germacrene-D (20.77%), a-Panasinsene (14.40 %) and b-Selinene (11.68 %). The essential oil presented antibacterial activity against 5 serotypes of Salmonella enterica, with MIC and MBC ranging from 7000 to 437 μg.mL-1. While MIC and MBC from the range from 200 to 25 mg.mL-1, being effective to most serotypes of S. enterica, with the exception of the aqueous extract. The results suggest that the essential oil and leaf extracts of Z. caribaeum represent an alternative for the control of S. enterica in the poultry sector, in this way reflecting a new perspective for studies with natural products.

https://doi.org/10.1590/s1519-99402017000300005 1493 downloads

Creation of a comprehensive proficiency testing program for molecular diagnosis in Brazil
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Barra, Gustavo B.

; Jácomo, Rafael H.

; Gomes, Jéssica S.

; Lopes, Rafael M.

Jornal Brasileiro de Patologia e Medicina Laboratorial

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jun 2017, Volume 53 N. 3 Pages 194 - 195

Abstract: En | Text: En | PDF: En

https://doi.org/10.5935/1676-2444.20170030 513 downloads

Performance of the Visual Analogue Scale of Happiness and of the Cornell Scale for Depression in Dementia in the Tremembé Epidemiological Study, Brazil
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César, Karolina G.

; Brucki, Sonia M.D.

; Takada, Leonel T.

; Nascimento, Luiz Fernando C.

; Gomes, Camila M.S.

; Almeida, Milena C.S.

; Oliveira, Maira O.

; Porto, Fábio H.G.

; Senaha, Mirna L.H.

; Bahia, Valéria S.

; Silva, Thaís Bento L.

; Ianof, Jéssica N.

; Spíndola, Lívia

; Schmidt, Magali T.

; Jorge, Mário S.

; Vale, Patrícia H.F.

; Cecchini, Mário A.

; Cassimiro, Luciana

; Soares, Roger T.

; Gonçalves, Márcia R.

; Smid, Jerusa

; Porto, Claudia S.

; Carthery-Goulart, Maria Teresa

; Yassuda, Mônica S.

; Mansur, Letícia L.

; Nitrini, Ricardo

Dementia & Neuropsychologia

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dec 2014, Volume 8 N. 4 Pages 389 - 393

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A depressão é um problema importante e crescente de saúde pública. É muito comum ser encontrada uma relação significativa entre depressão e a presença de distúrbios cognitivos nos estudos populacionais. Objetivo: Estabelecer a correlação entre a Escala Analógica Visual de Felicidade e a Escala Cornell de Depressão em Demência na população de 60 anos ou mais da cidade de Tremembé, estado de São Paulo, Brasil. Métodos: Estudo epidemiológico no qual foram realizadas visitas domiciliares na cidade de Tremembé. A amostra foi aleatória, através do sorteio de 20% da população acima de 60 anos de cada setor censitário do município. Este estudo foi de fase única, tendo sido realizada anamnese, exames físico e neurológico, avaliação cognitiva e aplicação de escalas Cornell de Depressão em Demência e Escala Analógica de Felicidade para verificar a presença de sintomas depressivos. Foi adotado como critério da presença de sintomas depressivos, pontuação maior ou igual a 8 na escala de Cornell. Resultados: Foram avaliadas 623 pessoas e destas 251 (40,3%) apresentaram sintomas depressivos significativos clinicamente na escala de Cornell, com associação significativa com gênero feminino (p<0,001) e com a baixa escolaridade (p=0,012). Cento e trinta e seis participantes (21,8%) apontaram para faces de infelicidade, com associação significativa com idade (p<0,001), com gênero feminino (p=0,020) e com baixo nível socioeconômico (p=0,012). Embora tenha havido significância estatística no teste de correlação, a correlação entre as duas escalas analisadas não foi alta (rho=0,47). Conclusão: A prevalência de sintomas depressivos foi elevada nesta amostra e a Escala Analógica de Felicidade e a Escala Cornell de Depressão em Demência não devem ser utilizadas como alternativas similares para avaliar a presença de sintomas depressivos, pelo menos em populações com baixa escolaridade.

Depression is a major growing public health problem. Many population studies have found a significant relationship between depression and the presence of cognitive disorders. OBJECTIVE: To establish the correlation between the Visual Analogue Scale of Happiness and the Cornell Scale for Depression in Dementia in the population aged 60 years or over in the city of Tremembé, state of São Paulo, Brazil. METHODS: An epidemiological survey involving home visits was carried out in the city of Tremembé. The sample was randomly selected by drawing 20% of the population aged 60 years or older from each of the city's census sectors. In this single-phase study, the assessment included clinical history, physical and neurological examination, cognitive evaluation, and application of both the Cornell Scale and the Analogue Scale of Happiness for psychiatric symptoms. The presence of depressive symptoms was defined as scores greater than or equal to 8 points on the Cornell Scale. RESULTS: A total of 623 subjects were evaluated and of these 251 (40.3%) had clinically significant depressive symptoms on the Cornell Scale, with a significant association with female gender (p<0.001) and with lower education (p=0.012). One hundred and thirty-six participants (21.8%) chose the unhappiness faces, with a significant association with age (p<0.001), female gender (p=0.020) and low socioeconomic status (p=0.012). Although there was a statistically significant association on the correlation test, the correlation was not high (rho=0.47). CONCLUSION: The prevalence of depressive symptoms was high in this sample and the Visual Analogue Scale of Happiness and Cornell Scale for Depression in Dementia should not be used as similar alternatives for evaluating the presence of depressive symptoms, at least in populations with low educational level.

https://doi.org/10.1590/S1980-57642014DN84000014 1582 downloads

Characteristics of Saccharomyces cerevisiae yeasts exhibiting rough colonies and pseudohyphal morphology with respect to alcoholic fermentation
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Reis, Vanda Renata

; Bassi, Ana Paula Guarnieri

; Silva, Jessica Carolina Gomes da

; Ceccato-Antonini, Sandra Regina

Brazilian Journal of Microbiology

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dec 2013, Volume 44 N. 4 Pages 1121 - 1131

Abstract: En | Text: En | PDF: En

Among the native yeasts found in alcoholic fermentation, rough colonies associated with pseudohyphal morphology belonging to the species Saccharomyces cerevisiae are very common and undesirable during the process. The aim of this work was to perform morphological and physiological characterisations of S. cerevisiae strains that exhibited rough and smooth colonies in an attempt to identify alternatives that could contribute to the management of rough colony yeasts in alcoholic fermentation. Characterisation tests for invasiveness in Agar medium, killer activity, flocculation and fermentative capacity were performed on 22 strains (11 rough and 11 smooth colonies). The effects of acid treatment at different pH values on the growth of two strains ("52" -rough and "PE-02" smooth) as well as batch fermentation tests with cell recycling and acid treatment of the cells were also evaluated. Invasiveness in YPD Agar medium occurred at low frequency; ten of eleven rough yeasts exhibited flocculation; none of the strains showed killer activity; and the rough strains presented lower and slower fermentative capacities compared to the smooth strains in a 48-h cycle in a batch system with sugar cane juice. The growth of the rough strain was severely affected by the acid treatment at pH values of 1.0 and 1.5; however, the growth of the smooth strain was not affected. The fermentative efficiency in mixed fermentation (smooth and rough strains in the same cell mass proportion) did not differ from the efficiency obtained with the smooth strain alone, most likely because the acid treatment was conducted at pH 1.5 in a batch cell-recycle test. A fermentative efficiency as low as 60% was observed with the rough colony alone.

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Prevalence of depressive symptoms among elderly in the city of Tremembé, Brazil: Preliminary findings of an epidemiological study
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César, Karolina G.

; Takada, Leonel T.

; Brucki, Sonia M.D.

; Nitrini, Ricardo

; Nascimento, Luiz Fernando C.

; Oliveira, Maira O.

; Gomes, Camila M.S.

; Almeida, Milena C.S.

; Porto, Fábio H.

; Senaha, Mirna L.H.

; Bahia, Valéria S.

; Yassuda, Mônica S.

; Silva, Thaís B.L.

; Ianof, Jéssica N.

; Spíndola, Lívia

; Schmidt, Magali T.

; Jorge, Mário S.

; Vale, Patrícia H.F.

; Cecchini, Mário A.

; Cassimiro, Luciana

; Soares, Roger T.

; Gonçalves, Márcia Rúbia

; Martins, Ana Caroline S.

; Rocha, Elisângela

; Daré, Patrícia

Dementia & Neuropsychologia

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sep 2013, Volume 7 N. 3 Pages 252 - 257

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Depressão é uma doença mental heterogênea classificada como um conjunto de transtornos, que se manifestam numa certa duração, frequência e intensidade. A prevalência de depressão em idosos varia de 0,5 a 16%. Objetivo: estabelecer a prevalência de sintomas depressivos significantes em estudo epidemiológico em população acima de 60 anos. Métodos: Estudo epidemiológico do tipo transversal, no qual estão sendo realizadas visitas domiciliares na cidade de Tremembé, Brasil. A amostra foi aleatória, através do sorteio de 20% da população acima de 60 anos de cada setor censitário do município. Este estudo é de única fase, sendo realizada anamnese, exames físico e neurológico, avaliação cognitiva e aplicação de escalas de Cornell e questionário Patient Health Questionnaire para verificar sintomas psiquiátricos. Foi adotado como critério da presença de sintomas depressivos, pontuação maior ou igual a 8 na escala de Cornell. Resultados: Foram avaliadas 455 pessoas e destas 169 (37,1%) apresentaram sintomas depressivos significativos clinicamente (SDSC). A maior prevalência foi entre as mulheres (p<0,001) e com escolaridade mais baixa (p=0,033). Quando realizado o teste de qui-quadrado de tendência, houve relação significativa, à medida que diminui o nível socioeconômico, aumenta a chance da presença de sintomas depressivos (p=0,005). Conclusão: A prevalência de sintomas depressivos foi elevada nesta amostra do estudo populacional e com associação com gênero feminino, baixo nível educacional e socioeconômico, mas há necessidade de finalizar toda amostragem.

ABSTRACT Depression is a heterogeneous mental disease classified as a set of disorders, which manifest with a certain duration, frequency and intensity. The prevalence of depression in the elderly ranges from 0.5 to 16%. Objective To establish, in an epidemiological study, the prevalence of significant depressive symptoms in the population aged 60 years or older. Methods: Results of a cross-sectional epidemiological study, involving home visits, being carried out in the city of Tremembé, Brazil, were reported. The sample was randomly selected by drawing 20% of the population over 60 years from each of the city's census sectors. In this single-phase study, the assessment included clinical history, physical and neurological examination, cognitive evaluation, the Cornell scale and the Patient Health Questionnaire for psychiatric symptoms. Scores greater than or equal to 8 on the Cornell scale were taken to indicate the presence of depressive symptoms. Results: A total of 455 elders were assessed, and of these 169 (37.1%) had clinically significant depressive symptoms (CSDS). Depression prevalence was higher among women (p<0.001) and individuals with lower education (p=0.033). The Chi-square test for trends showed a significant relationship where lower socioeconomic status was associated with greater likelihood of depressive symptoms (p=0.005). Conclusion: The prevalence of depressive symptoms was high in this sample of the population-based study and was associated with female gender, low educational level and socioeconomic status. The assessment of the entire population sample must be completed.

https://doi.org/10.1590/S1980-57642013DN70300004 1154 downloads

11.

Adherence to antiretroviral prophylaxis during early infancy in Latin America
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Hofer, Cristina B.

; Harris, D. Robert

; Saavedra, Mariza C.

; Haberer, Jessica E.

; Romeiro, Juliana

; Mussi-Pinhata, Marisa M.

; Stankievich, Erica

; Gomes, Ivete M.

; Kreitchmann, Regis

; Read, Jennifer S.

Brazilian Journal of Infectious Diseases

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aug 2013, Volume 17 N. 4 Pages 495 - 496

Abstract: En | Text: En | PDF: En

https://doi.org/10.1016/j.bjid.2012.11.006 2322 downloads

12.

Phenolic compounds from Sidastrum micranthum (A. St.-Hil.) fryxell and evaluation of acacetin and 7,4'-Di-O-methylisoscutellarein as motulator of bacterial drug resistence
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Gomes, Roosevelt A.

; Ramirez, Rafael R. A.

; Maciel, Jéssica Karina da S.

; Agra, Maria de Fátima

; Souza, Maria de Fátima Vanderlei de

; Falcão-Silva, Vivyanne S.

; Siqueira-Junior, José P.

Química Nova

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2011, Volume 34 N. 8 Pages 1385 - 1388

Abstract: En | Text: En | PDF: En

From the aerial parts of Sidastrum micranthum (A. St.-Hil.) Fryxell (Malvaceae) were isolated m-methoxy-p-hydroxy-benzaldehyde, o-hydroxy-benzoic acid, acacetin, quercetin, 7,4′-Di-O-methylisoscutellarein, genkwanin and tiliroside. These compounds were identified by data analyses of spectroscopic methods. Although acacetin and 7,4′-Di-O-methylisoscutellarein did not display relevant antibacterial activity (MIC = 256 µg/mL), they modulated the activity of antibiotics, i.e. in combination with antibiotics at 64 µg/mL (¼ MIC), a two-fold reduction in the MIC was observed for norfloxacin and ethidium bromide; regarding tetracycline and erythromycin a two-fold reduction in the MIC was observed only with 7,4′-Di-O-methylisoscutellarein.

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13.

Valores de referência e influência da idade no eritrograma de bubalinos da raça Murrah
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Gomes, Viviani

; Moura, Jéssica A.

; Madureira, Karina M.

; Baptistella, Fernanda

; Kitamura, Sandra S.

; Blagitz, Maiara G.

; Benesi, Fernando J.

Pesquisa Veterinária Brasileira

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apr 2010, Volume 30 N. 4 Pages 301 - 304

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O eritrograma é uma avaliação que representa um auxílio ao clínico no diagnóstico de afecções que acometem os animais domésticos. No entanto, estudos hematológicos em bubalinos são escassos, havendo poucas informações na literatura a respeito de valores de referência dos constituintes sanguíneos desta espécie. Assim, este trabalho teve como objetivo a análise do eritrograma de búfalos de diferentes faixas etárias da raça Murrah, machos e fêmeas. Os animais em estudo foram distribuídos em quatro grupos experimentais, de acordo com as idades: Grupo 1: animais com idade entre o nascimento e 3 meses (n=15); Grupo 2: animais com idade entre 4 e 6 meses (n=50); Grupo 3: animais com idade entre 7 e 12 meses (n=50); e Grupo 4: animais com idade superior a 12 meses (n=50). Como resultados das análises foram encontrados os seguintes valores médios, no Grupo 1: 7,9x106 hemácias/mL de sangue (He); 13,0 g/dL de hemoglobina (Hb); hematócrito (Ht) de 38,9%; Volume Corpuscular Médio (VCM) de 49,0 fl; Concentração Hemoglobínica Corpuscular Média (CHCM) de 33,6 %, e Hemoglobina Corpuscular Média (HCM) de 16,4 pg; no Grupo 2: He: 7,1 x10(6)/mL; Hb: 12,5 g/dL; Ht: 36,8%; VCM: 52,4 fl; CHCM: 33,9%, e HCM: 17,8 pg; no Grupo 3: He: 7,9 x10(6)/mL; Hb: 12,0 g/dL; Ht: 33,8%; VCM: 43,1 fl; CHCM: 35,4%, e HCM: 15,34 pg; e no Grupo 4: He: 6,7 x10(6)/mL; Hb: 11,7 g/dL; Ht: 34,4%; VCM: 53,4 fl; CHCM: 34,4%. e HCM: 17,4 pg. A análise estatística dos resultados encontrados para as diferentes faixas etárias permitiu concluir que ocorreram variações determinadas pela evolução da idade, caracterizadas por diminuição do número médio de hemácias (He), da concentração de hemoglobina (Hb) e do valor do hematócrito (Ht). Os índices hematimétricos apresentaram variações significativas no G3.

Erythrograms aid the veterinary practitioner in the diagnosis of diseases that affect domestic animals. However, hematological studies involving buffaloes are scarce, with little information in the literature on reference values for blood components in this species. Therefore, the objective of this study was analyze erythrograms of Murrah breed buffaloes of different ages, both males and females. Buffaloes in the study were distributed into four experimental groups, according to their ages: Group 1, animals from birth to 3 months of age (n=15); Group 2, animals from 4 to 6 months of age (n=50); Group 3, animals from 7 to 12 months of age (n=50); and Group 4, animals from 13 months to five years of age (n=50). The following values were obtained, for Group 1: 7.9x10(6) erythrocytes/mL (He), 13.0g/dL hemoglobin (Hb), hematocrit (Ht) 38.9%, Mean Corpuscular Volume (MCV) 49.0 fl, Mean Hemoglobin Corpuscular Concentration (MCHC) 33.6%, and Mean Corpuscular Hemoglobin (MCH) 16.4 pg; for Group 2: He7.1x10(6)/mL, Hb12.5g/dL, Ht 36.8%, MCV 52.4 fl; MCHC 33.9%; and MCH 17.8 pg; for Group 3: He 7.9x10(6)/mL, Hb12.0g/dL, Ht 33.8%, MCV 43.1 fl, MCHC 35.4%, and MCH 15.34pg; for Group 4: He 6.7x10(6)/mL, Hb 11.7g/dL, Ht 34.4%, MCV 53.4 fl, MCHC 34.4%, and MCH 17.4 pg. Statistical analysis of the results for the different age ranges permitted the conclusion that when the animals got older there was a decrease in the mean red cell counts (He), hemoglobin concentration (Hb) and hematocrit values (Ht). Although hematometric indexes showed significant variations in G3.

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