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au:GONZALEZ, MARIO H.

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Solventes eutéticos naturais profundos (NADES) no preparo de amostras de rocha fosfática e suplemento mineral para determinação elementar por técnicas de plasma
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Santana, Ana P. R.

; Andrade, Daniel F.

; Guimarães, Taciana G. S.

; Amaral, Clarice D. B.

; Oliveira, Andrea

; Nogueira, Ana R. A.

; Gonzalez, Mario H.

Química Nova

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jun 2021, Volume 44 N. 6 Pages 689 - 695

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Natural deep eutectic solvents (NADES) based on xylitol, citric acid, and malic acid were synthesized and used in ultrasound-assisted extraction (UAE) and heating-bath extraction of phosphate rock and mineral supplement samples. Arsenic, Ca, Cd, Cr, Cu, Fe, K, Mg, Mn, Na, P and Zn were determined by inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometry (ICP OES). The results showed the NADES as promising solvents for extraction of inorganic matrices compared to reference values and concentrations obtained using microwave-assisted acid digestion (MW-AD). Significant differences were observed for some elements, suggesting different chemical interactions between the synthesized NADES and each monitored element. For analytes extraction of phosphate rock, UAE presented the best results for As, Cr and P. Zinc was extracted with UAE and heating bath. Concerning mineral supplement, no significant differences were observed for Ca (UAE), Cd (UAE and heating bath), K (UAE and heating bath), Mn (UAE and heating bath), Na (UAE and heating bath), and P (UAE and heating bath). The NADES is a greener and a potentially alternative for the sample preparation compared to the official methods of analysis, can being used as selective extractor solvent in conventional sample preparation methods.

https://doi.org/10.21577/0100-4042.20170723 130 downloads

Toxicidad de insecticidas contra (Optatus palmaris Pascoe) en guanábana
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Hernández-Fuentes, Luis Martín

; Nolasco-González, Yolanda

; Orozco-Santos, Mario

; Montalvo-Gonzalez, Efigenia

Revista mexicana de ciencias agrícolas

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feb 2021, Volume 12 N. 1 Pages 49 - 60

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Originally from tropical America, the soursop (Annona muricata) has great potential and production expectations in Mexico. However, it presents pest problems that limit its cultivation. One of these is the fruit weevil (Optatus palmaris), whose damage detracts from quality and yield. Insecticides and repellants were evaluated: spinosad, spinoteram, azadirachtin, lambda-cyhalothrin, chlorpyrifos, imidacloprid and etofenprox. The percentage of mortality, fruit consumption was evaluated and the CL50 and TL50 were determined. One hour after application, 89.2% and 85.7% (α< 0.05) mortality were observed with lambda-cyhalothrin and chlorpyrifos at a concentration of 0.025 g ai L-1 and 2.4 g ai L-1, respectively. Spinosad and azadirachtin had no mortality effect at concentrations of 0.6 g ai L-1 and 32 g ai L-1, respectively (α< 0.05). At 12 h after the application spinosad (0.6 g ai L-1), lambda-cyhalothrin (0.0012 g ai L-1) and chlorpyrifos (0.24 g ai L-1) exerted more than 95% mortality (α< 0.05). Fruit consumption was lower with the application of lambda-cyhalothrin, 24.9% of consumed fruit (α< 0.05). The insecticide with the highest toxicity was imidacloprid with an CL50 of 0.06 g ai L-1 and TL50 of 0.3 h, followed by spinoteram with an CL50 of 0.96 g ai L-1 and a TL50 of 0.36 h. Azadirachtin did not cause mortality or decrease fruit consumption compared to the absolute control at concentrations of 0.32, 3.2 and 32 g ai L-1 (α< 0.05) therefore the use of this product is not recommended for the control of O. palmaris.

Resumen Originario de América tropical, el guanábano (Annona muricata) tiene gran potencial y expectativa de producción en México. No obstante, presenta problemas de plagas que limitan su cultivo. Uno de estos es el picudo del fruto (Optatus palmaris), cuyo daño demerita la calidad y rendimiento. Se evaluaron los insecticidas y repelentes: spinosad, spinoteram, azadiractina, lambda-cialotrina, clorpirifos, imidacloprid y etofenprox. Se evalúo el porcentaje de mortalidad, consumo de fruta y se determinaron la CL50 y TL50. Una hora después de la aplicación se observó 89.2% y 85.7% (α< 0.05) de mortalidad con lambda-cialotrina y clorpirifos en concentración de 0.025 g ia L-1 y 2.4 g ia L-1, respectivamente. Spinosad y azadiractina no tuvieron efecto de mortalidad en concentraciones de 0.6 g ia L-1 y 32 g ia L-1, respectivamente (α< 0.05). A las 12 h después de la aplicación spinosad (0.6 g ia L-1), lambda-cialotrina (0.0012 g ia L-1) y clorpirifos (0.24 g ia L-1) ejercieron más de 95% de mortalidad (α< 0.05). El consumo de fruta fue menor con aplicación de lambda-cialotrina, 24.9% de fruta consumida (α< 0.05). El insecticida con mayor toxicidad fue imidacloprid con una CL50 de 0.06 g ia L-1 y TL50 de 0.3 h, le siguió el spinoteram con una CL50 de 0.96 g ia L-1 y un TL50 de 0.36 h. Azadiractina no ejerció mortalidad ni disminuyó el consumo de fruta comparado con el testigo absoluto en concentraciones de 0.32, 3.2 y 32 g ia L-1 (α< 0.05) por lo que no se recomienda el uso de este producto para el control de O. palmaris.

https://doi.org/10.29312/remexca.v12i1.2417 22 downloads

Simple GFAAS Method for Determination of Pb, As, and Cd in Cannabidiol Extracts Used for Therapeutic Purposes
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Nascimento, Patricia A.

; Schultz, Juliana

; Gonzalez, Mario H.

; Oliveira, Andrea

Journal of the Brazilian Chemical Society

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may 2020, Volume 31 N. 5 Pages 894 - 903

Abstract: En | Text: En | PDF: En

Cannabis sativa has many promising medicinal applications for the mitigation, or even cure, of chronic diseases. The high bioaccumulation potential of C. sativa enables its use for the phytoremediation and detoxification of soil, may increase the levels of inorganic elements in products such as cannabidiol (CBD) extracts being necessary the evaluation/monitoring of the level of inorganic components, mainly the toxic species in these formulations. An analytical method employing graphite furnace atomic absorption spectrometry (GFAAS) was developed for the quantification of Pb, As, and Cd in commercial CBD extracts and homemade samples. The alkaline solubilization was employed in agreement with the external analytical curve, in a robust method. The limits of quantification were 0.26 (Pb), 0.067 (As), and 0.011 µg g-1 (Cd), with satisfactory accuracy (80-120%) and relative standard deviation (RSD) values < 7%. The CBD extracts presented levels of Pb, As and Cd below the maximum limits established by regulatory agencies.

https://doi.org/10.21577/0103-5053.20190252 1272 downloads

How are HCV-infected patients being identified in Brazil: a multicenter study
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Portari-Filho, Luiz H.

; Álvares-da-Silva, Mario R.

; Gonzalez, Aline

; Ferreira, Adalgisa P.

; Nogueira, Cristiane V.

; Mendes-Correa, Maria C.

; Lima, José M.

; Lopes, Edmundo P.

; Brandão, Carlos E.

; Ivantes, Cláudia

; Lyra, André

; Lindenberg, Andreia

; Ferraz, Maria L.

Brazilian Journal of Infectious Diseases

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jan 2019, Volume 23 N. 1 Pages 34 - 39

Abstract: En | Text: En | PDF: En

ABSTRACT Background: Hepatitis C is an important health problem. In Brazil, 1-2 million people are infected. Despite this expressive number, and the availability of very successful treatment, many patients remained undiagnosed mainly because of the asymptomatic nature of the infection. Objectives: To describe epidemiological characteristics of HCV-infected patients seen at referral centers in Brazil, the source of referral, and the time spanned to reach a reference center, in order to improve the identification of undiagnosed patients. Methods: Multicenter observational, cross-sectional study carried out in 15 centers of Brazil, between January/2016 and June/2017. Data of patients with a confirmed diagnosis (anti-HCV and HCV-RNA) were collected by interview using standard questionnaires and by review of charts. Results: Two thousand patients were included; 55.1% were male, mean age 58 ± 11 years. Only 14.9% had higher education and 84.2% received up to five monthly minimum Brazilian wages (approximately US$260.00/month). The time between diagnosis and beginning of follow-up was 22.9 months. The most common reasons for testing were check-up (33.2%) and blood donation (19%). General practitioners diagnosed most of the patients (30.1%). Fibrosis stage was mainly evaluated by liver biopsy (61.5%) and 31.3% of the patients were cirrhotic at diagnosis. Conclusions: This multicenter Brazilian study showed that the mean time to reach a referral center for treatment was almost two years. Primary care physicians diagnoses most hepatitis C cases in the country. Population campaigns and medical education should be encouraged to intensify screening of asymptomatic individuals, considering the efficiency of check-ups in identifying new patients.

https://doi.org/10.1016/j.bjid.2019.01.006 533 downloads

Efecto de los antimaláricos sobre los diferentes dominios del índice de daño SLICC en pacientes de la cohorte GLADEL
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Pons-Estel, Guillermo J.

; Quintana, Rosana

; Wojdyla, Daniel

; Alarcón, Graciela S.

; Serrano, Rosa María

; Ugarte-Gil, Manuel

; Pimentel-Quiroz, Víctor

; Soriano, Enrique R.

; Catoggio, Luis J.

; Scolnik, Marina

; Sacnun, Mónica

; Saurit, Verónica

; Caeiro, Francisco

; Alvarellos, Alejandro

; Sarano, Judith

; García, Mercedes

; Onetti, Laura

; Drenkard, Cristina

; Berbotto, Guillermo

; Scherbarth, Hugo R.

; Sato, Emilia

; Bonfa, Eloisa

; Ferreira Borba, Eduardo

; Costallat, Lilian

; Xavier, Ricardo

; Tavares Brenol, Joao C.

; Da Silva, Nilzio A.

; Cavalcanti, Fernando

; Massardo, Loreto

; Jacobelli, Sergio

; Neira, Oscar

; Molina, José F

; Vásquez, Gloria

; Gómez-Puerta, José A.

; Alonso Gonzalez, Luis

; Iglesias Gamarra, Antonio

; Guibert-Toledano, Marlene

; Reyes, Gil A.

; Cardiel, Mario H.

; Pascual-Ramos, Virginia

; García de la Torre, Ignacio

; Barile, Leonor

; Silveira, Luis H.

; Amigo, Mary-Carmen

; Sauza del Pozo, María Josefina

; Acevedo-Vásquez, Eduardo M.

; Alfaro-Lozano, José

; Segami, María Inés

; Chacón-Díaz, Rosa

; Abadi, Isaac

; Esteva Spinetti, María H

; Pons-Estel, Bernardo A.

Revista argentina de reumatología

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sep 2018, Volume 29 N. 3 Pages 6 - 10

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Objective: To assess the effects of antimalarials (AM) over the items of the SLICC Damage Index (SDI). Methods: Patients with recent (≤2 years) diagnosis of systemic lupus erythematosus (SLE) from the GLADEL cohort were studied. End-point: increase in items SDI since cohort entry. Independent variables (socio-demographic, clinical, laboratory and treatment) were included. The effect of AM as a time dependent variable on most frequent SDI items (adjusting for potential confounders) was examined with a multivariable Cox regression model. Results: Of the 1466 patients included in this analysis, 1049 (72%) received AM with a median exposure time of 30 months (Q1-Q3: 11-57). Damage occurred in 665 (45%) patients during a median follow-up time of 24 months (Q1-Q3: 8-55). There were 301 integument, 208 renal, 149 neuropsychiatric, 98 musculoskeletal, 88 cardiovascular and 230 others less frequently represented damages. After adjusting for potential confounders at any time during follow-up, a lower risk of renal damage (HR 0.652; 95% CI: 0.472-0.901) and borderline for neuropsychiatric damage (HR 0.701, 95% CI: 0.481-1.024) was found. Conclusion: In the GLADEL cohort, after adjustment for possible confounding factors, AM were independently associated with a reduced risk of renal damage accrual.

Objetivos: Estimar el efecto de los antimaláricos (AM) sobre los diferentes dominios del índice de daño SLICC (SDI). Métodos: Se estudiaron pacientes con diagnóstico clínico reciente (≤2 años) de lupus eritematoso sistémico (LES) de la cohorte GLADEL. Variable de estudio: aumento en los dominios del SDI desde el ingreso a la cohorte. Variables independientes: características sociodemográficas, clínicas, laboratorio y tratamientos. El efecto de los AM, como variable dependiente del tiempo, sobre los dominios más frecuentes del SDI (ajustado por factores de confusión) fue examinado con un modelo de regresión de Cox multivariado. Resultados: De 1466 pacientes estudiados, 1049 (72%) recibieron AM con un tiempo medio de exposición de 30 meses (Q1-Q3: 11-57) y 665 pacientes (45%) presentaron daño durante un seguimiento medio de 24 meses (Q1-Q3: 8-55); 301 eventos fueron cutáneos, 208 renales, 149 neuropsiquiátricos, 98 musculoesqueléticos, 88 cardiovasculares y 230 otros. Después de ajustar por factores de confusión, el uso de AM se asoció a un menor riesgo de daño renal (HR 0,652; IC 95%: 0,472-0,901) y en el límite de la significancia estadística (HR 0,701, IC 95%: 0,481-1,024) para el dominio neuropsiquiátrico. Conclusión: En GLADEL, el uso de AM se asoció independientemente a un menor riesgo de daño acumulado renal.

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Evaluation of Distribution and Bioaccumulation of Arsenic by ICP-MS in Tilapia (Oreochromis niloticus) Cultivated in Different Environments
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Oliveira, Luciano H. B.

; Ferreira, Nathália S.

; Oliveira, Andrea

; Nogueira, Ana Rita A.

; Gonzalez, Mario H.

Journal of the Brazilian Chemical Society

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dec 2017, Volume 28 N. 12 Pages 2455 - 2463

Abstract: En | Text: En | PDF: En

The concentration of arsenic in tissues of tilapia was evaluated and an estimation of the risks associated with the consumption of this fish was done, taking into account the guidelines established by FAO/WHO. The inductively coupled plasma mass spectrometry (ICP-MS) technique in kinetic energy discrimination (KED) mode was employed and certified reference materials were analyzed with recoveries of 101, 110, and 80% from NIST 1640a (trace elements in water), NIST 1566b (oyster tissue), and DORM-3 (fish protein). In the consumable portion (the muscle fish tissue), the average concentration found was 0.030 ± 0.008 µg g-1, which is below the arsenic maximum level established by international regulatory agencies. The average concentration found in the viscera (stomach, gills, and liver) was 0.485 ± 0.225 µg g-1, concluding that the viscera had greater capacity for the bioaccumulation of arsenic. From this study was possible to monitor the arsenic element in different tilapia specimens evidencing its accumulation throughout the body of the fish.

https://doi.org/10.21577/0103-5053.20170101 1150 downloads

Determination of Elemental Impurities in Acyclovir Ointment and Raw Materials Using Microwave Acid Digestion (MW-AD) and ICP-MS
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Gonzalez, Mario H.

; Silva, Caroline S. da

; Amaral, Clarice D. B.

; Bianchi, Silmara R.

; Oliveira, Luciano H. B. de

; Coelho, Jéssica S.

; Oliveira, Andrea

; Nogueira, Ana Rita A.

Journal of the Brazilian Chemical Society

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jan 2017, Volume 28 N. 1 Pages 98 - 105

Abstract: En | Text: En | PDF: En

Inorganic elements present in pharmaceutical products were determined by inductively coupled plasma-mass spectrometer (ICP-MS), employing microwave acid digestion as a sample preparation method. The most abundant isotopes, 75As+, 65Cu+, 52Cr+, 58Ni+, 208Pb+, and 51V+, were determined in acyclovir ointment and its constituents. Elemental impurities in the pharmaceutical samples were determined using an inductively coupled plasma-universal cell technology-mass spectrometer (ICP-UCT-MS) operated in kinetic energy discrimination (KED) and standard mode. The accuracy of the method was assessed by means of addition and recovery experiments. Except for Cr, spiked recoveries of the elements added to the samples before microwave acid digestion (MW-AD) step were between 95.1 and 126%, and after MW-AD step ranged from 85.6 to 132%. Limits of detection (LOD) obtained by proposed method is in agreement to those required by the U.S. Pharmacopeia Convention (USP), showing that the ICP-MS technique is well suited for quantification of these elements. The LOD's (ng L-1) for 51V+, 52Cr+, 58Ni+, 65Cu+, 75As+ and 208Pb+ in 1% v v-1 HNO3 medium were: 2.2, 81.3, 91.9, 6.9, 0.2 and 3.1, respectively. The concentration of the analytes in these samples presented a wide range, varying from 2.3 ± 0.8 µg kg-1 for As to 3446 ± 47 µg kg-1 for Cr.

https://doi.org/10.5935/0103-5053.20160151 1863 downloads

Construção de espectrômetro de emissão atômica com atomização eletrotérmica em filamento de tungstênio (WCAES)
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Gonzalez, Mário H.

; Santos, Luana N.

; Nóbrega, Joaquim A.

; Rocha, Fábio R. P.

Química Nova

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2010, Volume 33 N. 10 Pages 2266 - 2271

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

It is here discussed the development of a low cost analytical instrument with capacity for metals determination using atomic emission measurements in an electrothermal atomization system with a tungsten coil atomizer. The main goal was to show a new frontier for using this atomizer and to demonstrate that the simple instrumental arrangement here proposed has potential for portability and for solving analytical tasks related to metals determination. Atomic emission of calcium was selected for the adjustment of instrumental parameters and to evaluate the main characteristics of the lab-built instrument. Cobalt was determined in medicines and one alloy to demonstrate its feasibility.

3558 downloads

Clinical and radiological aspects in Melnick-Needles syndrome
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Albano, Lilian M. J.

; Chong, A. Kim

; Lee, Vivian K.

; Sugayama, Sofia M. M.

; Barba, Mário F.

; Utagawa, Cláudia Y.

; Bertola, Débora

; Gonzalez, Claudette H.

Revista do Hospital das Clínicas

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apr 1999, Volume 54 N. 2 Pages 69 - 72

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A síndrome de Melnick-Needles é uma displasia esquelética ligada ao X e letal no sexo masculino. Caracteriza-se pela presença de um fácies típico e dos seguintes achados radiológicos: esclerose dos ossos da base do crânio e mastóide, tíbia em forma de "S"; irregularidades corticais e costelas com aspecto de fita ("ribbon-like"). A maioria dos 48 casos já relatados na literatura (bem documentados), eram esporádicos, observando-se transmissão parental em apenas 11 famílias. Estudamos a primeira família brasileira com transmissão mãe-filha, cuja propósita apresentava o fácies e as manifestações radiológicas características da síndrome, além de uma restrição pulmonar importante e hipertensão pulmonar. Sua mãe, também afetada, mostrava um quadro clínico e radiológico mais leves.

Melnick-Needles syndrome is an X-linked dominant bone dysplasia, lethal in males, characterized by a typical facies and characteristic radiological findings: including sclerosis of skull base and mastoids, S-shaped appearance of tibia; cortical irregularities with a ribbon appearance of the ribs. About 48 well-documented cases have been reported, most of them were sporadic. Parental transmission has been published in only 11 kindreds. We are presenting the first Brazilian family with mother-daughter transmission.The proposita presented the typical clinical and radiological features with characteristic facies, severe thoracic cage restriction and pulmonary hypertension. Her mother was more mildly affected.

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