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au:GOUVEA, R.

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Degradation of Reactive Dyes by Thermally Activated Persulfate and Reuse of Treated Textile Dye-Bath
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Basilio, Amanda O.

; Dohler, Lucas

; Servin, Matheus

; Gouvea, Carlos A. K.

; Ribeiro, Ronny R.

; Peralta-Zamora, Patricio

Journal of the Brazilian Chemical Society

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sep 2021, Volume 32 N. 9 Pages 1865 - 1873

Abstract: En | Text: En | PDF: En

Due to the usual resistance of textile dyes to conventional biological treatment processes, the color removal of dyeing wastewaters remains a challenge for the textile industry. This work evaluates the capacity of advanced oxidation processes based on thermally-activated persulfate concerning textile dyes’ degradation in aqueous solution and the reuse of dyeing baths. Preliminary studies were carried out in a bench-scale jacketed reactor, using Reactive Black 5 (40 mg L−1) as a model dye. Almost complete dye degradation was observed in 60 min in this stage, using 300 mg L−1 of persulfate and activation temperatures of 80 ºC, basically due to the action of radical sulfate. The use of high concentrations of persulfate (1000 mg L−1) allowed efficient color removal of dyeing baths containing remazol dyes in processes activated at 80 and 90 ºC. However, the wastewaters treated under these conditions did not lead to high dyeing efficiency in reuse studies, probably due to residual persulfate presence. In contrast, low concentrations of persulfate (250 mg L−1) lead to partial color removal and a better dyeing quality. The results suggest a good potential for treating high-temperature dyeing baths, saving water and auxiliary agents used in textile dyeing processes.

https://doi.org/10.21577/0103-5053.20210077 37 downloads

Use and misuse of biomarkers and the role of D-dimer and C-reactive protein in the management of COVID-19: A post-hoc analysis of a prospective cohort study
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Gonçalves, Fabio Augusto Rodrigues

; Besen, Bruno Adler Maccagnan Pinheiro

; Lima, Clarice Antunes de

; Corá, Aline Pivetta

; Pereira, Antônio José Rodrigues

; Perazzio, Sandro Félix

; Gouvea, Christiane Pereira

; Fonseca, Luiz Augusto Marcondes

; Trindade, Evelinda Marramon

; Sumita, Nairo Massakazu

; Duarte, Alberto José da Silva

; Lichtenstein, Arnaldo

; Bonfa, Eloisa

; Utiyama, Edivaldo M.

; Segurado, Aluisio C.

; Perondi, Beatriz

; Miethke-Morais, Anna

; Montal, Amanda C.

; Harima, Leila

; Fusco, Solange R. G.

; Silva, Marjorie F.

; Rocha, Marcelo C.

; Marcilio, Izabel

; Rios, Izabel Cristina

; Kawano, Fabiane Yumi Ogihara

; Jesus, Maria Amélia de

; Kallas, Ésper George

; Carmo, Carolina

; Tanaka, Clarice

; Souza, Heraldo Possolo de

; Marchini, Julio F. M.

; Carvalho, Carlos

; Ferreira, Juliana C.

; Levin, Anna Sara Shafferman

; Oliveira, Maura Salaroli

; Guimarães, Thaís

; Lázari, Carolina dos Santos

; Sabino, Ester

; Magri, Marcello M. C.

; Barros-Filho, Tarcisio E. P.

; Francisco, Maria Cristina Peres Braido

; Costa, Silvia F.

Clinics

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2021, Volume 76 elocation e3547

Abstract: En | Text: En | PDF: En

OBJECTIVE: Coronavirus disease 2019 (COVID-19) is associated with high mortality among hospitalized patients and incurs high costs. Severe acute respiratory syndrome coronavirus 2 infection can trigger both inflammatory and thrombotic processes, and these complications can lead to a poorer prognosis. This study aimed to evaluate the association and temporal trends of D-dimer and C-reactive protein (CRP) levels with the incidence of venous thromboembolism (VTE), hospital mortality, and costs among inpatients with COVID-19. METHODS: Data were extracted from electronic patient records and laboratory databases. Crude and adjusted associations for age, sex, number of comorbidities, Sequential Organ Failure Assessment score at admission, and D-dimer or CRP logistic regression models were used to evaluate associations. RESULTS: Between March and June 2020, COVID-19 was documented in 3,254 inpatients. The D-dimer level ≥4,000 ng/mL fibrinogen equivalent unit (FEU) mortality odds ratio (OR) was 4.48 (adjusted OR: 1.97). The CRP level ≥220 mg/dL OR for death was 7.73 (adjusted OR: 3.93). The D-dimer level ≥4,000 ng/mL FEU VTE OR was 3.96 (adjusted OR: 3.26). The CRP level ≥220 mg/dL OR for VTE was 2.71 (adjusted OR: 1.92). All these analyses were statistically significant (p<0.001). Stratified hospital costs demonstrated a dose-response pattern. Adjusted D-dimer and CRP levels were associated with higher mortality and doubled hospital costs. In the first week, elevated D-dimer levels predicted VTE occurrence and systemic inflammatory harm, while CRP was a hospital mortality predictor. CONCLUSION: D-dimer and CRP levels were associated with higher hospital mortality and a higher incidence of VTE. D-dimer was more strongly associated with VTE, although its discriminative ability was poor, while CRP was a stronger predictor of hospital mortality. Their use outside the usual indications should not be modified and should be discouraged.

https://doi.org/10.6061/clinics/2021/e3547

Pharmacokinetic disposition of erythraline in rats after intravenous administration
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Demarque, Daniel P.

; Callejon, Daniel R.

; Pinto, Larissa G.

; Gouvea, Dayana R.

; de Moraes, Natália V.

; Lopes, João L.C.

; Lopes, José N.C.

; Lopes, Norberto P.

; Guaratini, Thais

Revista Brasileira de Farmacognosia

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dec 2019, Volume 29 N. 6 Pages 773 - 777

Abstract: En | Text: En | PDF: En

ABSTRACT Erythraline is the major alkaloid produced by Erythrina verna Vell., Fabaceae, a plant used in folk medicine and phytotherapeutic products to treat anxiety and sleep disorders. This study aimed to investigate the pharmacokinetic parameters of erythraline after intravenous administration in rats. For the analysis of erythraline in rat plasma, a method was developed and validated using UHPLC-MS/MS. Pharmacokinetic parameters were estimated by non-compartmental analysis. The metabolite 8-oxo-erythraline observed in previous biomimetic model studies was monitored during in vivo experiments. The quantification limit was 5 ng/ml within a linear range of 5-2000 ng/ml. After an intravenous dose of 1 mg/kg, the following pharmacokinetic parameters were observed: elimination half-life of 44.2 min; total clearance of 42.1 ml/min/kg and volume of distribution of 2085 ml/kg. In summary, a precise, accurate and selective UHPLC-MS/MS method was developed and successfully applied to investigate the pharmacokinetics of erythraline in rats. The metabolite 8-oxo-erythraline was observed in rat plasma after 20 min of erythraline administration.

https://doi.org/10.1016/j.bjp.2019.07.002 371 downloads

Somatic embryogenesis in the commercial papaya hybrid UENF/Caliman 01 relying on plantlet production from sexed adult hermaphrodite donor plants
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GOUVEA, DRIELLY S.

; CHAGAS, KRISTHIANO

; CIPRIANO, JAMILE L.D.

; LOPES, JOSÉ CARLOS

; SCHMILDT, EDILSON R.

; OTONI, WAGNER C.

; SCHMILDT, OMAR

; ARAUJO, CAROLINE P. DE

; ALEXANDRE, RODRIGO S.

Anais da Academia Brasileira de Ciências

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2019, Volume 91 N. 3 elocation e20180504

Abstract: En | Text: En | PDF: En

Abstract: Somatic embryogenesis from explants from hermaphrodite papaya mother plants is an alternative for the production of true-to-type plants without the need for sexing. This study aimed to analyze hormonal and osmotic inducers in different somatic embryogenesis stages in the commercial hermaphrodite hybrid papaya UENF/Caliman 01. Leaf disks from in vitro shoots originated from ex vitro hermaphrodite plants were cultured in induction medium supplemented with different concentrations of 2,4-D (6, 9, 12, 15, and 18 μM) and 4-CPA (19, 22, 25, 28, and 31 μM). After 90 days, the formation of somatic embryos was verified. The 2,4-D induced the formation of light brown calli with low frequency (20%) of somatic embryogenesis. However, 4-CPA (25 μM) induced 96% of embryogenic calli, which were transferred to maturation medium (MM) and cultured for 30 days. The MM contained ABA (0.5 μM) and AC (15 g L-1) and produced 36.6 somatic embryos callus-1, mainly on cotyledonary stage. Cotyledonary embryos were transferred to germination medium supplemented with gibberellic acid (GA3) (0.0, 1.44, 2.88, and 4.32 µM), and the conversion into plantlets was enhanced with GA3 at 2.88 µM.

https://doi.org/10.1590/0001-3765201920180504 1394 downloads

Effect of entomopathogens on Africanized Apis mellifera L. (Hymenoptera: Apidae)
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Potrich, Michele

; Silva, Rita T.L. da

; Maia, Fabiana M.C.

; Lozano, Everton R.

; Rossi, Robson M.

; Colombo, Fernanda C.

; Tedesco, Flavia G.

; Gouvea, Alfredo de

Revista Brasileira de Entomologia

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jan 2018, Volume 62 N. 1 Pages 23 - 28

Abstract: En | Text: En | PDF: En

ABSTRACT This study aimed to evaluate the effect of commercially used entomopathogens on Africanized Apis mellifera L. (Hymenoptera: Apidae). Four bioassays were performed: 1) pulverized entomopathogens on A. mellifera; 2) entomopathogens sprayed on a smooth surface; 3) entomopathogens sprayed on soy leaves; and 4) entomopathogens mixed with candy paste (sugar syrup). Five treatments were prepared: sterile distilled water (control), distilled water sterilized with Tween® 80 (0.01%), and the commercial entomopathogens Metarhizium anisopliae E9 (1.0 × 109 conidia mL−1), Beauveria bassiana PL63 (1.0 × 108 conidia mL−1) and Bacillus thuringiensis var. kurstaki HD-1 (3.0 × 108 spores mL−1). Each treatment consisted of five repetitions, with 20 workers per repetition, which were stored in a plastic box and, later, in a biological oxygen demand (B.O.D.) incubator (27 ± 2 ºC, RH of 60% ± 10%, 12-h photophase). The mortality of the workers was evaluated from 1 h to 240 h, and the data were analyzed using Bayesian inference. The workers killed by the ingestion of candy paste contaminated with the pathogens (products) were randomly separated and selected for the removal of the midgut. Each midgut was fixed in Bouin's solution and prepared for histology. B. bassiana was verified to reduce the survival of A. mellifera workers in all bioassays. Moreover, M. anisopliae reduced the survival of A. mellifera workers directly sprayed, on a smooth surface and mixed with candy. B. thuringiensis reduced A. mellifera survival on a smooth surface and mixed with candy paste. However, its effects were lower than that observed by B. bassiana. The treatments with the biological products did not induce morphometric alterations in the midgut of A. mellifera.

https://doi.org/10.1016/j.rbe.2017.12.002 951 downloads

Spatial and Temporal Variations in Secondary Metabolites Content of the Brazilian Arnica Leaves (Lychnophora ericoides Mart., Asteraceae)
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Gobbo-Neto, Leonardo

; Bauermeister, Anelize

; Sakamoto, Humberto T.

; Gouvea, Dayana R.

; Lopes, João Luis C.

; Lopes, Norberto P.

Journal of the Brazilian Chemical Society

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dec 2017, Volume 28 N. 12 Pages 2382 - 2390

Abstract: En | Text: En | PDF: En

The genus Lychnophora (Asteraceae) is a medicinal plant traditionally used as anti-inflammatory or analgesic agent. Its biological properties have been widely investigated and correlated with its chemical composition, which could be different between the species and even accordingly to the place and season of collection. In this work, it was investigated the spatial and temporal variations in the secondary metabolites from five populations of Lychnophora ericoides. Among them, a more diversified secondary metabolism was observed for the plants collected at Ibiraci-MG (Brazil), a city placed between two biomes, Cerrado and Atlantic Forest. The highest concentrations of chlorogenic acids were found in the Cerrado dry season (winter), while the flavonoids were higher during the rainy season (summer). Significant reduction was observed in the contents of all the secondary metabolites in old leaves, and also in flowery branches. These variations detected in L. ericoides leaves could highly influence the intensity of the biological activities of this plant.

https://doi.org/10.21577/0103-5053.20170092 1330 downloads

An UPLC-MS/MS Method for Determination of Vicenin-2 and Lychnopholic Acid in Rat Plasma and its Application to a Pharmacokinetic Study
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Gouvea, Dayana R.

; Buqui, Gabriela A.

; Lopes, João Luis C.

; Diniz, Andrea

; Lopes, Norberto P.

Journal of the Brazilian Chemical Society

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mar 2017, Volume 28 N. 3 Pages 427 - 434

Abstract: En | Text: En | PDF: En

Vicenin-2 (VIC) and lychnopholic acid (LA) are present in large amounts in hydroalcoholic extract from leaves of Lychnophora salicifolia, commonly known in the Brazilian Cerrado as "arnicão", a plant applied as topic anti-inflammatory and as a flavoring agent for the traditional spirit (cachaça). A new ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for quantification of LA and VIC in plasma samples in order to evaluate their pharmacokinetic profiles in rats. The method was linear in the range of 10-2500 ng mL-1, acceptable precision accuracies were obtained and no significant matrix effect was observed. The validated method was successfully applied to pharmacokinetic study. LA presented very low t1/2 (half-time), high Cl (serum clearance) and Vd (volume of distribution), and for VIC, the t1/2 value was slightly larger, but still rather low, whereas Cl and Vd values were slightly lower, indicating that both substances are instantaneous and well distributed in the body, and quickly eliminated.

https://doi.org/10.21577/0103-5053.20160249 866 downloads

Characterization of intestinal absorption of C-glycoside flavonoid vicenin-2 from Lychnophora ericoides leafs in rats by nonlinear mixed effects modeling
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Buqui, Gabriela A.

; Sy, Sherwin K.B.

; Merino-Sanjuán, Matilde

; Gouvea, Dayana R.

; Nixdorf, Suzana L.

; Kimura, Elza

; Derendorf, Hartmut

; Lopes, Norberto P.

; Diniz, Andrea

Revista Brasileira de Farmacognosia

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jun 2015, Volume 25 N. 3 Pages 212 - 218

Abstract: En | Text: En | PDF: En

AbstractVicenin-2 (apigenin-6,8-di-C-β-d-glucopyranoside) is present in hydroalcoholic extracts of the Brazilian species Lychnophora ericoides Mart., Asteraceae, leaves, and the biological effects of this compound have been demonstrated including anti-inflammatory, antioxidant and anti-tumor effects in rat models. Given the potential of this compound as a pharmacological agent, the aims of this investigation were to evaluate the extent of intestinal absorption of vicenin-2, and to determine the intestinal permeation profile using an in situ single-pass intestinal perfusion technique. A validated HPLC–UV method was applied to measure the amount of unabsorbed vicenin-2 in the gut after an oral administration of 180 mg kg-1 in five rats. A nonlinear mixed effects model was used to determine the absorption pharmacokinetic parameters assuming a first order absorption and active secretion processes for this compound, wherein the active secretion was characterized by a zero-order process. The population pharmacokinetic parameters obtained were 0.274 min-1 for the first-order absorption rate constant, 16.3% min-1 for the zero-order rate constant; the final percentage of the original dose that was absorbed in vivo was 40.2 ± 2.5%. These parameters indicated that vicenin-2 was rapidly absorbed in the small intestine. In contrast to literature information indicating no absorption of vicenin-2 in Caco-2 cells, our results suggested that vicenin-2 can be absorbed in the small intestine of rats. The finding supports further investigation of vicenin-2 as a viable oral phytopharmaceutical agent for digestive diseases.

https://doi.org/10.1016/j.bjp.2015.04.001 1338 downloads

Antiproliferative activity of Eremanthus crotonoides extracts and centratherin demonstrated in brain tumor cell lines
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Lobo, Jonathas F. R.

; Castro, Elaine S.

; Gouvea, Dayana R.

; Fernandes, Caio P.

; Almeida, Fernanda B. de

; Amorim, Lídia M. F. de

; Burth, Patrícia

; Rocha, Leandro

; Santos, Marcelo G.

; Harmerski, Lidilhone

; Lopes, Norberto P.

; Pinto, Angelo C.

Revista Brasileira de Farmacognosia

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dec 2012, Volume 22 N. 6 Pages 1295 - 1300

Abstract: En | Text: En | PDF: En

The genus Eremanthus is recognized by the predominance of sesquiterpene lactones from the furanoheliangolide type, a class of substances extensively tested against cancer cell lines. Thus, the species E. crotonoides (DC.) Sch. Bip., Asteraceae, obtained on "restinga" vegetation was evaluated against U251 and U87-MG glioma cell lines using the MTT colorimetric assay. Dichloromethane fraction was cytotoxic to both glioblastoma multiforme cell lines. We then conducted UPLC-PDA-ESI-MS/MS analysis of the dichloromethane fraction, which allowed the identification of the sesquiterpene lactones centratherin and goyazensolide. The isolation of centratherin was performed using chromatographic techniques and the identification of this substance was confirmed according to NMR data. Cytotoxic activity of centratherin alone was also evaluated against both U251 and U87-MG cells, which showed IC50 values comparable with those obtained for the commercial anticancer drug doxorubicin. All the tested samples showed cytotoxic activity against glioblastoma multiforme cells which suggests that E. crotonoides extracts may be important sources of antiproliferative substances and that the centratherin may serve as prototype for developing new antiglioblastoma drugs.

2931 downloads

10.

Sensitive method for determination of piplartine, an alkaloid amide from Piper species, in rat plasma samples by liquid chromatography-tandem mass spectrometry
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Bezerra, Daniel P.

; Pessoa, Cláudia

; Moraes, Manoel O.

; Costa-Lotufo, Letícia V.

; Rubio Gouvea, Dayana

; Jabor, Valquíria A. Polisel

; Lopes, Norberto Peporine

; Borges, Keyller Bastos

; Lima, Mary Anne S.

; Silveira, Edilberto R.

Química Nova

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2012, Volume 35 N. 3 Pages 460 - 465

Abstract: En | Text: En | PDF: En

Piplartine (PPTN) is an alkaloid amide found in Piper species that presents different activities. PPTN determination in rat plasma is necessary to better understand its biological effects. The aim of this study was to develop a sensitive LC-MS/MS method for the determination of PPTN in rat plasma. The performance criteria for linearity, sensitivity, precision, accuracy, recovery, and stability have been assessed and were within the recommended guidelines. The validated method proved to be suitable in a pilot study of PPTN kinetic disposition in rat plasma after a single intraperitoneal dose, and represents an appropriate tool to further pharmacokinetic studies.

2651 downloads

11.

Determinação das energias de superfície do SnO2 puro e dopado
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Castro, R. H. R.

; Hidalgo, P.

; Gouvea, D.

; Navrotsky, A.

Cerâmica

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dec 2009, Volume 55 N. 336 Pages 342 - 348

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A calorimetria tem se mostrado uma importante ferramenta para o controle e entendimento de comportamentos de sistemas nanoestruturados. Isto se deve basicamente às recentes medidas de energia de superfície reportadas na literatura; isto é, como em sistemas nanométricos a superfície tem uma maior contribuição na energia livre total do sistema, o conhecimento desta entalpia é fundamental para alcançar o controle desejado. No entanto, procedimentos calorimétricos para medida da energia de superfície são relativamente complexos e, se fosse possível estimar a energia de superfície por métodos alternativos, isto poderia ser de grande importância para o desenvolvimento da nanociência. Neste trabalho a energia de superfície do SnO2 é apresentada por medida de calorimetria de dissolução e corrigida por calorimetria de adsorção de água. Após isso, uma discussão sobre a influência de aditivos iônicos na área de superfície específica do SnO2 é apresentada. Quando os aditivos iônicos formam excesso de superfície no SnO2, a área de superfície aumenta mesmo considerando o aumento difusional esperado. Isto pode ser relacionado com a diminuição da energia de superfície pelos aditivos devido ao excesso de superfície como previsto por Gibbs. Baseado nesta premissa e utilizando o valor medido de energia de superfície do SnO2, as medidas de área de superfície dos pós dopados são utilizadas para calcular a energia de superfície de pós dopados com Ni, Fe, Cr e Mg.

Calorimetry has been proved to be an important tool to control and understand the behavior of nanostructured systems. This is basically because of the recent surface energy measurements reported in the literature by high-temperature calorimetry. That is, since in nanosized systems the surface accounts for a larger part of the total atoms, knowing their energy is of main importance to achieve a desired control. However, calorimetric procedures to measure the surface energy are relatively complex and, if it were possible to estimate the surface energy using alternative techniques, this could have giant impacts in the nanoscience and nanotechnology. In this work, the anhydrous surface energy of SnO2 is presented to be 3.2 J/m² measured by drop solution calorimetry corrected by water adsorption. After this, a discussion of the effect of some additives in the SnO2 nanostructure is presented. It is established that additives that form surface excess can decrease the surface energy in addition to modifications in the diffusion parameters. Considering a particle growth model, we could calculate the surface energy of SnO2 doped with Mg, Fe, Cr, and Ni.

2801 downloads

12.

A glimpse on biological activities of tellurium compounds
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Cunha, Rodrigo L. O. R.

; Gouvea, Iuri E.

; Juliano, Luiz

Anais da Academia Brasileira de Ciências

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sep 2009, Volume 81 N. 3 Pages 393 - 407

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O telúrio é um elemento não-essencial raro que vem sendo considerado tóxico, e o seu papel biológico é ainda pouco esclarecido. Apesar disso, os efeitos biológicos do telúrio elementar e de alguns derivados inorgânicos e orgânicos que têm sido estudados revelam um conjunto de aplicações diversificadas interessantes e promissoras. Como exemplo, pode-se destacar os usos de teluritos e teluratos de metais alcalinos em microbiologia, o efeito antioxidante de teluretos e diteluretos orgânicos, os efeitos imunomodulatórios e a diversidade de usos correlacionados a este efeito de uma telurana inorgânica denominada AS-101. Ademais, as aplicações de teluranas orgânicas (organoteluranas) como inibidoras de proteases e as aplicações em modelos de doenças compõem a mais recente contribuição ao cenário dos efeitos e aplicações biológicas do telúrio e seus compostos discutidas neste manuscrito.

Tellurium is a rare element which has been regarded as a toxic, non-essential trace element and its biological role is not clearly established to date. Besides of that, the biological effects of elemental tellurium and some of its inorganic and organic derivatives have been studied, leading to a set of interesting and promising applications. As an example, it can be highlighted the uses of alkali-metal tellurites and tellurates in microbiology, the antioxidant effects of organotellurides and diorganoditellurides and the immunomodulatory effects of the non-toxic inorganic tellurane, named AS-101, and the plethora of its uses. Inasmuch, the nascent applications of organic telluranes (organotelluranes) as protease inhibitors and its applications in disease models are the most recent contribution to the scenario of the biological effects and applications of tellurium and its compounds discussed in this manuscript.

11878 downloads

13.

Use of an ELISA system for detection of equine herpesvirus 1 (EHV-1) antibodies in non-symptomatic pregnant mares and neonatal foals
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Carvalho, R.

; Passos, L.M.F.

; Gouvea, A.M.G.

; Resende, M.

; Martins, A.S.

; Franco, G.C.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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jun 2000, Volume 52 N. 3 Pages 200 - 207

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Um sistema de ELISA foi utilizado para se estudar a prevalência do herpesvirus eqüino 1 (HVE-1) em plantéis de cavalos brasileiros vacinados e não vacinados. Amostras de soros foram coletadas de éguas gestantes assintomáticas, antes e após o parto e de seus respectivos potros recém-nascidos. O antígeno foi preparado de uma fração viral purificada (HVE-1 amostra Ab1). O ponto de corte (absorvância de 0,3516) foi determinado considerando o limite máximo para os valores negativos. Esse valor determinou sensibilidade de 100% e especificidade de 94,7%, utilizando um banco de soros de éguas gestantes e fetos. No estudo observou-se alta correlação entre o ELISA e a soro-neutralização para detecção de anticorpos específicos contra o EHV-1.

An ELISA system was applied to study the prevalence of EHV-1 in vaccinated and non-vaccinated Brazilian horses. Serum samples were taken from non-symptomatic pregnant mares, before and after the parturition, and from their neonatal foals. The antigen was prepared from a purified virus fraction (HVE-1 AB1 strain). The ELISA absorbance of 0.3516 was determined as the threshold of negative values. This cut-off level was defined from a selected interval, which determined a sensibility of 100% and a specificity of 94.7%, from a serum collection obtained from pregnant mares and fetuses. In this study, a strong correlation was found between the ELISA and serum neutralization for specific EHV-1 antibody detection.

3328 downloads Cited 2 times in SciELO

14.

As Apocynaceae da região de Poços de Caldas, Minas Gerais, Brasil
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Vasconcellos, Marina Bragatto

; Gouvea, Luiza Sumiko Kinoshita

Acta Botanica Brasilica

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jul 1993, Volume 7 N. 1 Pages 107 - 127

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Este trabalho consiste no estudo florístico da familia Apocynaceae em Poços de Caldas, dada sua representatividade na região e o valor fitoquímico e ornamental de várias de suas espécies. Foram encontradas 17 espécies distribuídas entre 8 gêneros: Aspidosperma Mart e Zucc., Condylocarpon Desf., Forsteronia G.F.W. Mey., Macrosiphonia Muell. Arg., Mandevilla Lindl., Peltastes R.E. Woodson, Prestonia R. Br. e Rauvolfia L.

A floristic study of the family Apocynaceae im Poços de Caldas was carried out, since the family is well represented in the region and many species have phytochermical and ornamental value. The family is represented by 17 species distributed in 8 genera: Aspidosperma Mart e Zucc., Condylocarpon Desf., Forsteronia G.F.W. Mey., Macrosiphonia Muell. Arg., Mandevilla Lindl., Peltastes R.E. Woodson, Prestonia R. Br. and Rauvolfia L.

https://doi.org/10.1590/S0102-33061993000100006 2602 downloads Cited 2 times in SciELO

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