RESUMO: Campilobacteriose Genital Bovina (CGB) é uma doença venérea e subclínica que causa problemas reprodutivos em rebanhos, causada por Campylobacter fetus subsp. venerealis. Este trabalho teve como objetivo selecionar peptídeos miméticos ao agente da CGB de uma biblioteca de fagos. Phage display é uma técnica que aplica bibliotecas de bacteriófagos que expõem peptídeos fusionados ao capsídeo viral em seleções biológicas contra proteínas alvo. Biopannings foram realizados para seleção biológica na biblioteca de fagos por meio de soro hiperimune de coelho e extrato proteico de C. fetus subsp. venerealis. Cinco heptapeptídeos selecionados foram considerados miméticos para Cfv-NCTC 10354 a partir de análises de bioinformática e ensaios com soro hiperimune e muco cérvico-vaginal de novilhas. ALASLPL e LSYLFPP foram os peptídeos mais reativos e considerados promissores como possíveis imunógenos miméticos para C. fetus subsp. venerealis.

ABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal and subclinical disease that affects the fertility of cattle herds, and it is caused by Campylobacter fetus subsp. venerealis . This study selected peptides mimetic to the BGC-causing agent from a phage library. Phage display is a technique that applies bacteriophage libraries that reveal peptides fused to the viral capsid in biological selections against target proteins. Biopannings were performed for biological selection in the phage library using rabbit hyperimmune serum and C. fetus subsp. venerealis protein extract. Five selected heptapeptides were considered mimetic to Cfv-NCTC 10354 based on the results of bioinformatics analysis and assays with hyperimmune serum and cervicovaginal mucus obtained from heifers. ALASLPL and LSYLFPP were the most reactive peptides and considered promising as possible mimetic immunogens for C. fetus subsp. venerealis.

RESUMO: O objetivo deste estudo foi avaliar a adesão e invasão de células HeLa por amotras de Campylobacter spp. (total n=63) isoladas de frangos (n=4), cães (n=4), primatas não-humanos (n=16), porcos (n=9), fezes de bezerros (n=18), e trato genital de bovinos (n=12). Trinta e duas amostras foram capazes de aderir e 13 invadiram células HeLa. As amostras invasivas incluíram 1 de 4 isolados de cão, 4 de 16 isolados de primatas não-humano (2 C. jejuni e 2 C. coli), 1 de 9 C. coli isoladas de porcos e 7 de 18 C. fetus subsp. fetus isoladas de fezes de bezerros. Apenas 25% dos isolados de frango e de cão e 23% dos isolados de suínos foram capazes de aderir a células HeLa, propriedade exibida por 65% das cepas obtidas a partir de fezes de bezerros e por 83% das amostras isoladas de trato genital bovino. O fenótipo aderente foi observado em 5 de 19, 6 de 15 e 21 de 29 amostras de C. jejuni, C. coli e C. fetus subsp. fetus, respectivamente, enquanto que 3 de 19, 3 de 15 e 7 de 29 amostras foram adicionalmente capazes de invadir as células HeLa, respectivamente. Amostras de C. jejuni, C. coli e C. fetus subsp. fetus isoladas de fezes de animais foram capazes de aderir e invadir as células HeLa, enquanto amostras de C. fetus subsp. fetus isoladas a partir de amostras de trato genital bovino não foram invasivas, em células HeLa. O presente estudo mostrou que amostras de C. jejuni isoladas de primatas não-humanos e cães, C. coli isoladas de primatas não-humanos e porcos, e C. fetus subsp. fetus isolados a partir de fezes de bezerros foram capazes de aderir e invadir células HeLa. Além disso, a falta de capacidade invasiva de amostras de C. fetus subsp. fetus isoladas de trato genital bovino pode ser importante na patogênese das doenças das vias genitais causadas por esta bactéria.

ABSTRACT: The objective of this study was to evaluate the adherence to and invasion of HeLa cells by Campylobacter spp. strains (total n=63) isolated from chickens (n=4), dogs (n=4), non-human primates (n=16), pigs (n=9), calf feces (n=18), and bovine genital tracts (n=12). Thirty-two strains adhered to and 13 invaded HeLa cells. Invasive strains included 1 of 4 dog isolates, 4 of 16 non-human primate isolates (2 C. jejuni and 2 C. coli), 1 of 9 C. coli strains isolated from pigs, and 7 of 18 C. fetus subsp. fetus isolated from calf feces. Only 25% of chicken and dog isolates and 23% of pig isolates were able to adhere to HeLa cells, a property of 65% of strains obtained from calf feces and 83% of bovine genital tract-isolated strains. The adherent phenotype was observed in 5 of 19, 6 of 15, and 21 of 29 strains of C. jejuni, C. coli, and C. fetus subsp. fetus, respectively, whereas 3 of 19, 3 of 15, and 7 of 29 strains were additionally able to invade HeLa cells, respectively. C. jejuni, C. coli, and C. fetus subsp. fetus strains isolated from animal feces are able to adhere and invade HeLa cells, whereas C. fetus subsp. fetus strains isolated from the bovine genital tract were not invasive in HeLa cells. The present study showed that C. jejuni isolated from primates and dogs, C. coli isolated from non-human primates and pigs, and C. fetus subsp. fetus isolated from calf feces have the ability to adhere to and to invade HeLa cells. Moreover, the lack of invasive ability by C. fetus subsp. fetus strains isolated from the bovine genital tract could be important in the pathogenesis of the genital tract diseases caused by this bacterium.

RESUMO. Doze novilhos canulados no rúmen (401,0 ± 41,5 kg) e 24 meses de idade foram usados em delineamento de quadrado latino truncado replicado com seis animais, seis tratamentos e quatro períodos, para avaliar o efeito da glicerina bruta (GB; 80,3% de glicerol) com amido ou ingredientes energéticos a base de fibra no concentrado sobre o CMS, digestibilidade da MS (DMS) e FDN (DFDN) e parâmetros ruminais. O período experimental foi 19 dias (14 dias de adaptação e 5 dias de coleta). As dietas foram: CO - sem glicerina bruta e milho como ingrediente do concentrado; GBM - GB (10% da MS) com milho no concentrado; GBCS - GB (10% da MS) com casca de soja no concentrado. As três dietas foram ofertadas em baixo (BC) ou alto (AC) teor de concentrado (TC; 40 ou 60%). Animais alimentados com BC ou AC apresentaram similar CMS, DMS e DFDN. Animais alimentados com GB com milho ou CS apresentaram maior concentração de propionato e menor relação A:P. Dietas com AC aumentou a concentração de propionato mas não afetou a DFDN. GB (10% da MS) pode ser usada para substituir o milho ou CS em dietas com 40 ou 60% de concentrado, sem afetar a DFDN.

ABSTRACT. Twelve ruminally cannulated steers (401.0 ± 41.5 kg) and 24 mo were used in a replicated arrangement truncated Latin Square with six animals in six treatments and four periods to evaluate the effect of crude glycerine (CG; 80.3% of glycerol) with starch or fiber-based energy ingredients in the concentrate on DMI, DM (DMD) and NDF digestibility (NDFD) and ruminal parameters. Experimental periods were 19 days (14 days for adaptation and 5 days to sampling). Diets were: CO - without CG and corn as ingredient of concentrate; CGC - inclusion of CG (10% of DM) with corn in the concentrate; and CGSH - inclusion of CG (10% of DM) with soybean hulls (SH) in the concentrate. All three diets were offered at low (LC) or high (HC) concentrate level, CL (40 or 60%). Animals fed LC or HC diets had similar DMI, DMD and NDFD. Animals fed diets with CG associated with corn or SH had higher propionate concentrations and lower A:P ratio. Diets with HC increase the propionate but do not affect the NDFD. CG (10% of DM) can be used to replace corn or SH in diets with 40 or 60% of concentrate, without affect NDFD.

RESUMO. Foram estudadas as características qualitativas de cinco músculos, maturados ou não, de quatorze bovinos da raça Nelore com 450 kg ± 30,7 kg de peso corporal e confinados por 60 dias com dieta contendo 60% de concentrado e 40% de volumoso na MS. Após o abate e resfriamento das carcaças, amostras dos músculos: picanha, (Biceps femoris - BF), contrafilé (Longissimus - LD), alcatra (Gluteus medius - GM), lagarto (Semitendinosus - ST) e acém (Trapezius thoracis - TT) foram retirados, embalados à vácuo e maturados em câmara B.O.D (0 a 2oC) por um, sete e 14 dias e posteriormente armazenadas a -20oC para análises químicas e de qualidade. Os dados foram analisados em um delineamento em split-plot utilizando o procedimento PROC MIXED do SAS. A maturação influencia nas características qualitativas e químicas da carne bovina, melhorando aspectos como a maciez (p < 0,01) e a luminosidade (p < 0,01). O músculo Trapezius thoracis tem uma maior estabilidade de cor quando comparado com o músculo Longissimus. Não é recomendada a maturação do músculo ST em razão deste não apresentar melhora em sua maciez. Os músculos GM e BF apresentam-se macios logo no primeiro dia, sendo que o processo de maturação para estes cortes é indicado em razão de agregar valor à carne.

ABSTRACT. This trial aimed to assess the qualitative traits of five muscles of 14 Nellore bulls with 450 kg ± 30.7 kg BW, feedlot fed for 60 days. After slaughter and carcass chilling, samples of the Biceps femoris (BF), Longissimus (LD), Gluteus medius (GM), Semitendinosus (ST) and Trapezius thoracis (TT) muscles were collected, vacuum-packed and aged at 0 to 2 ºC for 1, 7 and 14 days and subsequently stored at -20ºC for chemical and quality analysis. The pH, shear force, water holding capacity, cooking losses, meat color and ether extract were evaluated in all aging times. Data were analyzed in a split-plot design using the PROC MIXED procedure of SAS. Aging affected quality traits and chemical properties of the meat, improving aspects, such as tenderness (p < 0.01) and lightness (p < 0.01). The Trapezius thoracis muscle has a higher color stability compared with the Longissimus muscle. Aging is not recommended for the ST muscle because the tenderness is not improved. The GM and BF muscles are tender on the first day, and the aging process is indicated for these cuts for adding value to the beef.

Objetivou-se avaliar a inclusão de 10% de glicerina bruta (GB) associada a fontes de volumosos sobre a qualidade da carne maturada em 24 horas, sete e 14 dias post mortem de tourinhos da raça Nelore. Utilizaram-se 30 animais confinados (n = 10), com peso inicial de 416,70 ± 24,74 kg e 18 meses de idade, sendo submetidos aos três tratamentos: silagem de milho (SM), cana-de-açúcar (CA) ou bagaço de cana (BC), utilizando delineamento inteiramente casualizado. Após 85 dias, os animais foram abatidos com 554,51 ± 38,51 kg. Amostras do longissimus foram coletadas após resfriamento das carcaças e embaladas a vácuo. A dieta influenciou o pH, a coloração da carne e da gordura subcutânea (GS; p < 0,05). Animais alimentados com SM apresentaram maior valor de b* na GS (p < 0,05). A dieta não modificou o perfil de ácidos graxos (AG) da carne (p > 0,05). A maturação influenciou pH e força de cisalhamento da carne (FC; p < 0,05). Carnes maturadas aos 14 dias apresentaram maior pH (5,90) e menor FC (2,40 kgf). Dietas contendo 10% GB na MS associada à SM, CA ou BC não modificam os AG da carne. A maturação em 14 dias reduz a FC do longissimus, melhorando a qualidade da carne.

This trial aimed to evaluate the inclusion of 10% of crude glycerin associated with roughage sources on the quality of meat aged for 1, 7 or 14 days post mortem of Nellore young bulls. Thirty feedlot animals (n = 10) with initial body weight of 416.70 ± 24.74 kg and 18 months of age were assigned to three treatments: corn silage (CS), sugar cane (SC) and sugar cane bagasse (CB), using a completely randomized design. After 85 days of feeding, animals were slaughtered with 554.51 ± 38.51 kg. Samples of longissimus muscle were collected, after carcass chilling, and vacuum-packed. Diets influenced pH, meat color and subcutaneous fat (SF) (p > 0.05). Animals fed CS showed higher values of b* in SF (p < 0.05). Differences were not found in the meat fatty acid profile (p > 0.05). Aging times influenced pH and shear force of beef (p < 0.05). Beef aged for 14 days showed higher pH (5.90) and lower shear force (2.40 kgf). Diets containing 10% crude glycerin in the DM associated with CS, SC or CB had no effect on the fatty acid profile in beef. The aging process for 14 days reduces shear force, improving meat quality.

Objetivou-se neste trabalho avaliar a qualidade da carne maturada de tourinhos Nelore, sob dois graus de acabamento (GA) de carcaça. Foram utilizados 14 animais, com aproximadamente 450 ± 30 kg de peso corporal. A espessura de gordura subcutânea (EGS) dos animais foi avaliada no início do período experimental. Foram escolhidos 14 animais com EGS de 0-3 mm e 3,1-6 mm, formando-se dois grupos ao final do experimento: animais com acabamento entre 3-6 mm (sete animais) e entre 6,1-10 mm (sete animais). A cada 28 dias foram avaliados a EGS entre a 12-13ª costelas com ajuda de unidade ultrassonográfica. Os valores de coloração da carne, pH, perdas por cocção e força de cisalhamento não tiveram efeito de interação entre os tratamentos (p > 0,05). Não houve diferença significativa entre o GA sobre o comprimento de sarcômero (p = 0,11). Houve interação entre GA e maturação sobre a capacidade de retenção de água (p < 0,01). Os graus de acabamento avaliados não interferem na qualidade da carne, mas carcaças com acabamento entre 6,1-10 mm apresentam maior capacidade de retenção de água. A maturação do músculo longissimus em até 14 dias melhora a maciez da carne.

This trial aimed to evaluate the quality of aged beef from Nellore young bulls under two yield grade (YG). Fourteen animals with approximately 450 ± 30 kg body weight were evaluated for backfat thickness (BFT) at the beginning of the experimental period. Seven animals had BFT of 0-3 mm and seven animals, 3.1-6 mm. Two groups were formed at the end of the experiment: animals finished with 3-6 mm BFT (seven animals) and animals finished with 6.1 to 10 mm BFT (seven animals). Every 28 days, we evaluated by ultrasound the BFT between the 12nd 13rd ribs. There was no interaction between YG and aging for beef color, pH, cooking losses and shear force (p > 0.05). There was no effect of YG on sarcomere length (p = 0.11). However, there was interaction between YG and aging on water holding capacity (p < 0.01). The yield grades evaluated did not interfere with meat quality, but carcasses with 6.1 - 10 mm of backfat thickness showed highest water holding capacity. The aging of the longissimus muscle for up to 14 days improves beef tenderness.

Para a produção de anticorpos monoclonais contra Campylobacter fetus subsp. venerealis foram utilizadas as linhagens de células de mieloma Sp2/0-Ag14 e células de baço de camundongos BALB/c imunizados com sonicado de C. fetus subsp. venerealis NCTC 10354. A detecção dos anticorpos monoclonais foi realizada por ELISA indireto utilizando antígeno sonicado de C. fetus subsp. venerealis NCTC 10354. A clonagem foi realizada por diluição limitante e os clones foram caracterizados por ELISA indireto utilizando um painel de bactérias escolhidas em função da prevalência e habitats: C. fetus subsp. venerealis NCTC 10354, C. fetus subsp. fetus ADRI 1812, C. sputorum biovar sputorum LMG 6647, C. lari NCTC 11352 e Arcobacter skirrowii LMG 6621; e no "western blotting" utilizando antígenos sonicados de C. fetus subsp. venerealis NCTC 10354 e C. sputorum biovar sputorum LMG 6647. Foram obtidos 15 clones produtores de anticorpos anti- C. fetus subsp. venerealis das classes IgM (1) e IgG (14). Quatro clones dentre os 15 clones obtidos foram produtores de anticorpos monoclonais espécie-específicos: dois clones reagiram com maior especificidade contra C. fetus subsp. venerealis NCTC 10354 e dois clones reagiram com maior especificidade contra C. fetus subsp. fetus ADRI 1812. Nenhum dos clones reagiu contra C. sputorum biovar sputorum LMG 6647, comprovando a especificidade dos anticorpos monoclonais testados. Todos os clones reconheceram uma proteína de massa molecular de aproximadamente 148 kDa no sonicado de C. fetus subsp. venerealis NCTC 10354.

Myeloma cells Sp2/0-Ag14 and spleen cells from BALB/c mouse immunized with sonicated Campylobacter fetus subsp. venerealis NCTC 10354 were fused with polyethylene glycol (PEG) for the selection of clones producing antibodies. Clones were obtained by limiting dilution and screened for the production of specific antibodies to C. fetus subsp. venerealis NCTC 10354 by indirect ELISA and western blot against a panel of bacteria: C. fetus subsp. venerealis NCTC 10354, C. fetus subsp fetus ADRI 1812, C. sputorum biovar sputorum LMG 6647, C. lari NCTC 11352, and Arcobacter skirrowii LMG 6621 for the ELISA and C. fetus subsp. venerealis NCTC 10354 and C. sputorum biovar sputorum LMG 6647 for the western blotting. Fifteen clones producing monoclonal antibodies (MAbs) anti-C. fetus subsp. venerealis of the IgM (1) and IgG (14) classes were further screened for species-specificity. Four clones of the 15 obtained were producers of species-specific monoclonal antibodies (MAbs): two were specific for C. fetus subsp. venerealis and two were specific for C. fetus subsp. fetus. None of the clones were reactive against C. sputorum biovar sputorum LMG 6647. All clones recognized a protein with molecular mass of approximately 148 kDa from lysed C. fetus subsp. venerealis NCTC 10354.

O presente estudo avalia a interferência do teste de tuberculinização no teste do interferon gama (INFg), estima a sensibilidade e a especificidade do INFg em condições brasileiras e simula a utilização dos testes múltiplos usando a tuberculinização comparada e o teste do INFg. Trezentos e cinquenta animais oriundos de dois rebanhos livres e dois rebanhos positivos foram submetidos à tuberculinização comparada e ao teste de INFg. A tuberculinização comparada foi realizada utilizando PPD aviária e bovina. O teste de INFg foi realizado utilizando o kit Bovigam® (CSL Veterinary, Austrália) de acordo com as especificações do fabricante. A sensibilidade e especificidade do teste de INFg foram calculadas pelo Modelo Bayesiano de Classe Latente. Esses parâmetros foram também estimados para os testes múltiplos. Os resultados do teste de INFg no D0 e D3 após o teste de tuberculinização foram comparados pelos testes estatísticos de McNemar e kappa. Os resultados das médias de densidade ótica do teste de INFg em ambos os dias foram similares. A sensibilidade e a especificidade do teste de INFg apresentaram resultados variando (95% intervalo de confiança) de 72 a 100% e 74 a 100%, respectivamente. A sensibilidade do teste em paralelo foi acima de 97,5% enquanto a especificidade do teste em série foi acima de 99,7%. O teste do INFg provou ser um método de diagnóstico muito útil.

This study aimed to evaluate the interference of tuberculin test on the gamma-interferon (INFg) assay, to estimate the sensitivity and specificity of the INFg assay in Brazilian conditions, and to simulate multiple testing using the comparative tuberculin test and the INFg assay. Three hundred-fifty cattle from two TB-free and two TB-infected herds were submitted to the comparative tuberculin test and the INFg assay. The comparative tuberculin test was performed using avian and bovine PPD. The INFg assay was performed by the BovigamTM kit (CSL Veterinary, Australia), according to the manufacturer's specifications. Sensitivity and specificity of the INFg assay were assessed by a Bayesian latent class model. These diagnostic parameters were also estimate for multiple testing. The results of INFg assay on D0 and D3 after the comparative tuberculin test were compared by the McNemar's test and kappa statistics. Results of mean optical density from INFg assay on both days were similar. Sensitivity and specificity of the INFg assay showed results varying (95% confidence intervals) from 72 to 100% and 74 to 100% respectively. Sensitivity of parallel testing was over 97.5%, while specificity of serial testing was over 99.7%. The INFg assay proved to be a very useful diagnostic method.

We performed spoligotyping and 12-mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTRs) typing to characterise Mycobacterium bovis isolates collected from tissue samples of bovines with lesions suggestive for tuberculosis during slaughter inspection procedures in abattoirs in Brazil. High-quality genotypes were obtained with both procedures for 61 isolates that were obtained from 185 bovine tissue samples and all of these isolates were identified as M. bovis by conventional identification procedures. On the basis of the spoligotyping, 53 isolates were grouped into nine clusters and the remaining eight isolates were unique types, resulting in 17 spoligotypes. The majority of the Brazilian M. bovis isolates displayed spoligotype patterns that have been previously observed in strains isolated from cattle in other countries. MIRU-VNTR typing produced 16 distinct genotypes, with 53 isolates forming eight of the groups, and individual isolates with unique VNTR profiles forming the remaining eight groups. The allelic diversity of each VNTR locus was calculated and only two of the 12-MIRU-VNTR loci presented scores with either a moderate (0.4, MIRU16) or high (0.6, MIRU26) discriminatory index (h). Both typing methods produced similar discriminatory indexes (spoligotyping h = 0.85; MIRU-VNTR h = 0.86) and the combination of the two methods increased the h value to 0.94, resulting in 29 distinct patterns. These results confirm that spoligotyping and VNTR analysis are valuable tools for studying the molecular epidemiology of M. bovis infections in Brazil.

Foram identificadas diferenças no perfil protéico das amostras de Sponselee, Norma e Hardjoprajitno, com bandas protéicas entre 175, 47 kDA e 12,10 kDa. A amostra Sponselee foi a que apresentou maior número de bandas (12), seguida da Norma que apresentou 11 bandas e de Hardjoprajitno que apresentou 9 bandas. Todas as bandas observadas na amostra Sponselee possuíam correspondentes nas outras duas amostras. A amostra Norma não apresentou uma banda em torno de 35,77 kDa e a Hardjoprajitno não apresentou bandas em torno de 89,59 kDa, 35,77 kDa e 12,10 kDa. O reconhecimento dessas proteínas por soros hiperimunes contra cada uma das amostras também mostrou diferenças, sendo que o maior número de proteínas reconhecido em todas as amostras por todos os soros se encontrou entre 35,83 kDa e 29,19 kDa. Os soros contra bovino na amostra Norma só reconheceu proteínas de baixa massa molecular nas amostras Norma (6,80 kDa) e Hardjoprajitno (6,80 kDa e 5,30 kDa). Soro bovino contra a amostra Hardjoprajitno reconheceu uma proteína de 44,33 kDa todas às amostras e proteínas de 4,22 kDa nas amostras Sponselee e Norma e de 10,49 kDa e 6,16 kDa na Hardjoprajitno. As diferentes proteínas identificadas poderiam se constituir em alvos específicos para o desenvolvimento de testes diagnósticos e vacinas contra leptospirose bovina.

Differences in the protein profile of Leptospira sp. strains Sponselee, Norma and Hardjoprajitno were observed, with bands ranging from 175.47 kDa to 12.10 kDa. Strain Sponselee presented a 12-band profile, while strain Norma showed 11 and strain Hardjoprajitno showed 9 bands in the profile. All bands observed in Sponselee strain profile could match bands in the other two strains. Strain Norma lacks a band at 35.77 kDa and strain Hardjoprajitno lacks the bands at 89.59 kDa, 35.77 kDa and 12.10 kDa. The recognition profile from hyperimmune sera was also different for the studied serovar Hadjo strains. The majority of recognized proteins was in the range of 35.83 kDa to 29.19 kDa. Cattle sera against strain Norma only recognized low molecular mass proteins in strains Norma (6.80 kDa) and Hardjoprajitno (6.80 kDa and 5.30 kDa). Bovine sera against strain Hardjoprajitno recognized a 44.33 kDa protein in all studied strains and proteins of 4.22 kDa in strains Sponselee and Norma and of 10.49 kDa and 6.16 kDa in strain Hadjoprajitno. The different identified proteins could become specific targets to the development of diagnostic tests and vaccines against bovine leptospirosis.

One aspect the literature overlooks on the effects of labor regulation of labor market efficiency is the degree of the legislation's effectiveness, i.e., its actual enforcement in daily work relations. Even the more sophisticated econometric studies (which take into account the effects of interaction between labor market regulatory institutions in explaining its dynamics) leave this central issue aside, namely enforcement versus non-enforcement of the law. Keeping this issue in mind, we seek to answer the following question in this article: given that the effectiveness of labor legislation depends on the interaction between the overall sanctions and the probability of the employer getting caught breaking the law, and given that the law's effectiveness is a decisive aspect for the real measurement of a country's labor costs, to what extent is the Brazilian labor inspection system designed to meet its objective, namely, to enforce the law?

L'un des aspects négligés dans la littérature concernant les effets de la régulation du travail sur l'efficacité des marchés du travail est le degré d'action réelle de la loi, c'est-à-dire sa vraie portée dans les relations du travail de chaque jour. Des études économétriques pourtant relativement élaborées, qui tiennent compte des effets d'interaction entre institutions régulatrices du marché du travail dans l'explication de leur fonctionnement, ignorent la question centrale qui est l'obéissance ou non à la loi. Dans cet article on se pose la question: puisque la force de la législation du travail découle de l'effet d'interaction entre le montant des sanctions et les chances qu'a l'employeur d'être pris en faute; et puisque l'action effective de la loi est un aspect décisif pour une exacte mesure des coûts du travail d'un pays, dans quelle mesure le système d'inspection du travail au Brésil est pensé pour atteindre son objectif, qui est celui de faire respecter la loi?

One aspect the literature overlooks on the effects of labor regulation of labor market efficiency is the degree of the legislation's effectiveness, i.e., its actual enforcement in daily work relations. Even the more sophisticated econometric studies (which take into account the effects of interaction between labor market regulatory institutions in explaining its dynamics) leave this central issue aside, namely enforcement versus non-enforcement of the law. Keeping this issue in mind, we seek to answer the following question in this article: given that the effectiveness of labor legislation depends on the interaction between the overall sanctions and the probability of the employer getting caught breaking the law, and given that the law's effectiveness is a decisive aspect for the real measurement of a country's labor costs, to what extent is the Brazilian labor inspection system designed to meet its objective, namely to enforce the law?

L'un des aspects négligés dans la littérature concernant les effets de la régulation du travail sur l'efficacité des marchés du travail est le degré d'action réelle de la loi, c'est-à-dire sa vraie portée dans les relations du travail de chaque jour. Des études économétriques pourtant relativement élaborées, qui tiennent compte des effets d'interaction entre institutions régulatrices du marché du travail dans l'explication de leur fonctionnement, ignorent la question centrale qui est l'obéissance ou non à la loi. Dans cet article on se pose la question: puisque la force de la législation du travail découle de l'effet d'interaction entre le montant des sanctions et les chances qu'a l'employeur d'être pris en faute; et puisque l'action effective de la loi est un aspect décisif pour une exacte mesure des coûts du travail d'un pays, dans quelle mesure le système d'inspection du travail au Brésil est pensé pour atteindre son objectif, qui est celui de faire respecter la loi?

Transmission of Mycobacterium bovis from cattle to humans has been reported and can cause tuberculosis (Tb) and a problem in certain risk populations. Therefore, knowledge of resistance of M. bovis towards antibiotics used for therapy of human Tb could help avoiding cure delay and treatment cost increase when dealing with drug resistant organisms. We therefore evaluated the susceptibility of M. bovis isolates towards streptomycin, isoniazide, rifampicin, ethambutol, and ethionamide, the first line antibiotics for human Tb. Therefore, 185 clinical samples from cattle with clinical signs of tuberculosis were processed and submitted to culturing and bacterial isolates to identification and drug susceptibility testing using the proportion method. Among 89 mycobacterial strains, 65 were identified as M. bovis and none were resistant to any of the antibiotics used. Confirmation of present results by future studies, enrolling a large number of isolates and designed to properly represent Brazilian regions, may favor the idea of using isoniazide preventive therapy as part of a Tb control strategy in special situations. Also, nucleic acids from bacterial isolates were submitted to rifoligotyping, a recently described reverse hybridization assay for detection of mutations causing resistance towards rifampicin. Concordance between the conventional and the molecular test was 100%, demonstrating the use of such methodology for rapid evaluation of drug susceptibility in M. bovis.