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1.
Brain-derived neurotrophic factor serum levels following ketamine and esketamine intervention for treatment-resistant depression: secondary analysis from a randomized trial
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Caliman-Fontes, Ana Teresa
; Leal, Gustavo C.
; Correia-Melo, Fernanda S.
; Paixao, Camilla S.
; Carvalho, Michelle S.
; Jesus-Nunes, Ana Paula
; Vieira, Flavia
; Magnavita, Guilherme
; Bandeira, Igor D.
; Mello, Rodrigo P.
; Beanes, Graziele
; Silva, Samantha S.
; Echegaray, Mariana
; Carvalho, Lucas P.
; Machado, Paulo
; Sampaio, Aline S.
; Cardoso, Taiane de A.
; Kapczinski, Flávio
; Lacerda, Acioly L. T.
; Quarantini, Lucas C.
.
Abstract Objectives Evidence suggests that ketamine’s influence on brain-derived neurotrophic factor (BDNF) might be involved in its mechanism of rapid antidepressant action. We aimed to evaluate the differential impact of ketamine and esketamine on serum BDNF levels and its association with response patterns in treatment-resistant depression (TRD). Methods Participants (n = 53) are from a randomized, double-blind clinical trial comparing the efficacy of single-dose ketamine (0.5mg/kg, n = 27) and esketamine (0.25mg/kg, n = 26) in TRD. Depression severity was assessed before and 24 hours, 72 hours, and 7 days after the intervention, using the Montgomery-Åsberg Depression Rating Scale (MADRS). Blood samples were collected before infusion, 24 hours, and 7 days afterwards. Results There were no significant changes in BDNF levels at post-infusion evaluation points, and no difference in BDNF levels comparing ketamine and esketamine. Both drugs exhibited similar therapeutic effect. There was no association between BDNF levels and response to treatment or severity of depressive symptoms. Conclusion There was no significant treatment impact on BDNF serum levels – neither with ketamine nor esketamine – despite therapeutic response. These results suggest that ketamine or esketamine intervention for TRD has no impact on BDNF levels measured at 24 hours and 7 days after the infusion. This clinical trial is registered on the Japan Primary Registries Network: UMIN000032355.
2.
Diretriz da Sociedade Brasileira de Cardiologia sobre a Análise e Emissão de Laudos Eletrocardiográficos – 2022
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Samesima, Nelson
; God, Epotamenides Good
; Kruse, Jose Claudio Lupi
; Leal, Marcelo Garcia
; Pinho, Claudio
; França, Francisco Faustino de A. C.
; Pimenta, João
; Cardoso, Acácio Fernandes
; Paixão, Adail
; Fonseca, Alfredo
; Pérez-Riera, Andrés R.
; Ribeiro, Antonio Luiz Pinho
; Madaloso, Bruna Affonso
; Luna Filho, Bráulio
; Oliveira, Carlos Alberto Rodrigues de
; Grupi, César José
; Moreira, Dalmo Antonio Ribeiro
; Kaiser, Elisabeth
; Paixão, Gabriela Miana de Mattos
; Feitosa Filho, Gilson
; Pereira Filho, Horacio Gomes
; Grindler, José
; Aziz, José Luiz
; Molina, Marcos Sleiman
; Facin, Mirella
; Tobias, Nancy M. M. de Oliveira
; Oliveira, Patricia Alves de
; Sanches, Paulo César R.
; Teixeira, Ricardo Alkmin
; Atanes, Severiano Melo
; Pastore, Carlos Alberto
.
3.
PERFIL DE ELEMENTOS INORGÂNICOS EM AMOSTRAS DE MEL DO ESTADO DO MARANHÃO COMO UM POTENCIAL BIOINDICADOR DE ORIGEM GEOGRÁFICA
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Honey is a substance that contains a variety of macro and micro minerals present in the range between 0.02-1.03%. The main objective of this study was to determine the concentration of elements inorganic in 15 honey samples collected in various regions of Maranhão (Northeast Brazil), using highly sensitive analytical techniques, such as inductively coupled plasma optical emission spectrometry (ICP-OES), in addition to applying multivariate statistical tools, such as Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) in the treatment of data. The method presented detection limits between 0.4 to 2.8 mg kg-1. Fifteen samples and 19 metals were analyzed, and two of these samples had high levels of lead, exceeding the limits allowed by Brazilian legislation. Thus, it can be concluded that the presence of potentially toxic metals in honey is an indication that the production areas may be contaminated by different external sources. The chemometric analysis of the data by PCA and HCA allowed to classify honey in two main groups: honey produced in urban areas and honey produced in rural areas with PCA explaining 73% of the data variance with the sum of the first 3 components.
4.
Stigma toward individuals with mental disorders among Brazilian psychiatrists: a latent class analysis
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da Silva, Antônio G.
; Loch, Alexandre A.
; Leal, Vanessa P.
; da Silva, Paulo R.
; Rosa, Monike M.
; Bomfim, Ozeias da C.
; Malloy-Diniz, Leandro F.
; Schwarzbold, Marcelo L.
; Diaz, Alexandre P.
; Palha, Antônio P.
.
Objective: The stigma toward individuals with mental disorders is highly prevalent, not only in the general population but among health care providers as well. The aim of this study was to identify subgroups based on stigmatizing beliefs related to psychiatric disorders among Brazilian psychiatrists, as well as to investigate their association with clinical and personality characteristics. Methods: Latent cluster analysis was used to find subgroups of cases in multivariate data according to a psychotic (schizophrenia) and a nonpsychotic disorder (attention-deficit hyperactivity disorder). The clusters for each psychiatric disorder were compared according to sociodemographic, emotional traits, and personality characteristics. Results: A total of 779 psychiatrists answered the questionnaire. Three different subgroups of stigma levels were identified regarding schizophrenia: the highest (n=202 [51.7%]), intermediate (108 [27.6%]), and the lowest (81 [20.7%]). Participants from the highest stigma group had a significantly longer time since graduation, higher anxiety-state scores, and lower positive affect. Two subgroups were identified with respect to attention-deficit hyperactivity disorder, although there were no differences between them in sociodemographic or clinical variables. Conclusion: There were more subgroups of stigmatizing beliefs regarding psychotic disorders. Individual characteristics, such as those related to trait anxiety and affect, can be associated with high stigma toward schizophrenia.
https://doi.org/10.1590/1516-4446-2020-0864
221 downloads
5.
Land-use changes affect the functional structure of stream fish assemblages in the Brazilian Savanna
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Alvarenga, Ludmilla R. P.
; Pompeu, Paulo S.
; Leal, Cecília G.
; Hughes, Robert M.
; Fagundes, Daniela C.
; Leitão, Rafael P.
.
Resumo Investigamos os mecanismos envolvidos na relação entre mudanças de uso da terra e biodiversidade aquática, utilizando a ictiofauna de riachos do Cerrado como modelo de estudo. Testamos a predição de que a degradação da paisagem reduz a heterogeneidade ambiental e muda os tipos predominantes de habitat, por sua vez, diminuindo a diversidade e alterando a identidade funcional de comunidades de peixes. Amostramos 40 riachos da bacia do Alto Rio Paraná, e avaliamos as condições da drenagem e do habitat físico local. Em seguida, conduzimos uma análise ecomorfológica para caracterizar funcionalmente todas as espécies (36) e quantificar diferentes facetas da estrutura funcional das comunidades. Detectamos múltiplos caminhos de impacto das alterações da paisagem sobre a ictiofauna. A degradação das bacias de drenagem reduziu complexidade do leito e heterogeneidade no sombreamento pelo dossel, diminuindo especialização e divergência funcional das comunidades. Alterações na paisagem também reduziram volume de água e quantidade de pedras grandes nos riachos, resultando em diminuição na abundância de espécies de maior porte e com atributos morfológicos que favorecem a natação na coluna d’água. Concluímos que a intensificação dos usos da terra causa alterações significativas para a biodiversidade aquática no Cerrado, reforçando a necessidade de especial atenção a este hotspot global.
Abstract We investigated the mechanisms involved in the relationship between land-use changes and aquatic biodiversity, using stream fish assemblages of the Brazilian Savanna (i.e., Cerrado) as a study model. We tested the prediction that landscape degradation would decrease environmental heterogeneity and change predominant physical-habitat types, which in turn would decrease the functional diversity and alter the functional identity of fish assemblages. We sampled fish from 40 streams in the Upper Paraná River basin, and assessed catchment and instream conditions. We then conducted an ecomorphological analysis to functionally characterize all species (36) and quantify different facets of the functional structure of assemblages. We detected multiple pathways of the impacts from landscape changes on the fish assemblages. Catchment degradation reduced the stream-bed complexity and the heterogeneity of canopy shading, decreasing assemblage functional specialization and divergence. Landscape changes also reduced the water volume and the amount of large rocks in streams, resulting in decreased abundances of species with large bodies and with morphological traits that favor swimming in the water column. We conclude that land-use intensification caused significant changes in aquatic biodiversity in the Cerrado, reinforcing the need to pay special attention to this global hotspot.
https://doi.org/10.1590/1982-0224-2021-0035
178 downloads
6.
SARS-CoV-2 reinfection caused by the P.1 lineage in Araraquara city, Sao Paulo State, Brazil
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Romano, Camila Malta
; Felix, Alvina Clara
; Paula, Anderson Vicente de
; Jesus, Jaqueline Góes de
; Andrade, Pamela S.
; Cândido, Darlan
; Oliveira, Franciane M. de
; Ribeiro, Andreia C.
; Silva, Francini C. da
; Inemami, Marta
; Costa, Angela Aparecida
; Leal, Cibele O. D.
; Figueiredo, Walter Manso
; Pannuti, Claudio Sergio
; Souza, William M. de
; Faria, Nuno Rodrigues
; Sabino, Ester Cerdeira
.
Revista do Instituto de Medicina Tropical de São Paulo
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ABSTRACT Reinfection by the severe acute respiratory syndrome coronavirus type 2 (SARS-COV-2) has been reported in many countries, suggesting that the virus may continue to circulate among humans despite the possibility of local herd immunity due to massive previous infections. The emergence of variants of concern (VOC) that are more transmissible than the previous circulating ones has raised particular concerns on the vaccines effectiveness and reinfection rates. The P.1 lineage was first identified in December 2020 in Manaus city and is now globally spread. We report the first case of reinfection of SARS-CoV-2 caused by the P.1 variant outside of Manaus. The potential of these new variants to escape naturally and vaccine- induced immunity highlights the need for a global vigilance.
https://doi.org/10.1590/s1678-9946202163036
1125 downloads
7.
Difficulties in activities of daily living are associated with stigma in patients with Parkinson’s disease who are candidates for deep brain stimulation
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da Silva, Antônio G.
; Leal, Vanessa P.
; da Silva, Paulo R.
; Freitas, Fernando C.
; Linhares, Marcelo N.
; Walz, Roger
; Malloy-Diniz, Leandro F.
; Diaz, Alexandre P.
; Palha, Antônio P.
.
Objective: Parkinson’s disease (PD) is often accompanied by stigma, which could contribute to a worse prognosis. The objective of this study is to identify the variables associated with stigma in PD patients who are candidates for deep brain stimulation (DBS). Methods: We investigated sociodemographic and clinical variables associated with stigma in a sample of 54 PD patients indicated for DBS. The independent variables were motor symptoms assessed by the Movement Disorder Society‐sponsored revision of the Unified Parkinson Disease Rating Scale (MDS-UPDRS III), depressive symptoms measured by the Hospital Anxiety and Depression Scale, age, disease duration and the presence of a general medical condition. The Mobility, Activities of daily living and Emotional well-being domains of the 39-item Parkinson’s Disease Questionnaire (PDQ-39) were also investigated as independent variables, and the Stigma domain of the PDQ-39 scale was considered the outcome variable. Results: After multiple linear regression analysis, activities of daily living remained associated with the Stigma domain (B = 0.42 [95%CI 0.003-0.83], p = 0.048). The full model accounted for 15% of the variance in the Stigma domain (p = 0.03). Conclusions: Although causal assumptions are not appropriate for cross-sectional studies, the results suggest that ADL difficulties could contribute to greater stigma in PD patients with refractory motor symptoms who are candidates for DBS.
https://doi.org/10.1590/1516-4446-2018-0333
641 downloads
8.
Differences in protein expression associated with ivermectin resistance in Caenorhabditis elegans
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Sousa, Dauana Mesquita
; Cunha, Nivea Maria Ferreira da
; Silva, Deisianne Rodrigues da
; Aragão, Paulo de Tarso Teles Dourado de
; Aguiar, Mônica Valéria de Almeida
; Lobo, Marina Duarte Pinto
; Moreira, Ana Cristina de Oliveira Monteiro
; Cunha, Rodrigo Maranguape Silva da
; Miranda, Rodrigo Rodrigues Cambraia de
; Bevilaqua, Claudia Maria Leal
.
Revista Brasileira de Parasitologia Veterinária
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Resumo A administração indiscriminada de anti-helmínticos sintéticos, como a ivermectina, contribui para a seleção de subpopulações capazes de resistir ao efeito das drogas. Para entender os mecanismos de resistência à ivermectina em Caenorhabditis elegans, este estudo visou identificar alvos moleculares. Portanto, linhagens de C. elegans sensíveis e resistentes à ivermectina foram utilizadas. Os nematóides coletados foram adicionados ao tampão de extração e macerados em nitrogênio líquido para obtenção das proteínas. As proteínas extraídas foram separadas por peso molecular em SDS-PAGE para verificar sua integridade. Posteriormente, as proteínas de ambas as linhagens foram separadas por eletroforese bidimensional. Os géis foram analisados, os spots relevantes foram excisados e identificados por espectrometria de massa (NanoESI-Q-TOF e MASCOT®), em seguida, analisados em seus termos de GO e STRING®. A expressão aumentada de proteínas associadas à alta atividade metabólica, como as proteínas ATP-2 e ENOL-1, responsáveis pela síntese de ATP, foi observada. Além disso, foram identificadas as proteínas responsáveis pelo controle da função muscular (MLC-1, ACT-1 e PDI-2), sinalização (FAR-1 e FAR-2) e desenvolvimento embrionário (VHA-2). A análise das interações proteicas indicou que a maioria das proteínas identificadas na cepa resistente participa da mesma reação desencadeada pela ivermectina.
Abstract The indiscriminate administration of synthetic anthelmintics such as ivermectin contributes to the selection of subpopulations capable of resisting the drugs’ effects. To understand the mechanisms of ivermectin resistance in Caenorhabditis elegans, this study attempted to identify molecular targets. C. elegans lineages that were sensitive and resistant to ivermectin were used. Collected nematodes were added to an extraction buffer and macerated in liquid nitrogen for protein extraction. The extracted proteins were separated according to molecular weight by SDS-PAGE to verify their integrity. Subsequently, proteins from both lineages were separated using two-dimensional electrophoresis. The gels were analyzed and the relevant spots were excised and identified by mass spectrometry (NanoESI-Q-TOF and MASCOT®) and subsequently assessed by GO enrichment and STRING® analyses. The increased expression of proteins associated with high metabolic activity, such as ATP-2 and ENOL-1, which are responsible for ATP synthesis, was observed. Furthermore, proteins with involvement in mediating muscular function (MLC-1, ACT-1, and PDI-2), signaling (FAR-1 and FAR-2), and embryo development (VHA-2) were identified. Protein interaction analysis indicated that the majority of the identified proteins in the resistant lineages participated in the same reaction triggered by ivermectin.
https://doi.org/10.1590/s1984-29612019013
1256 downloads
9.
Growth of seedlings and young plants of coffee in composts of textile industry residues
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Neiva Júnior, Eudes
; França, André C.
; Grazziotti, Paulo H.
; Porto, Douglas W. B.
; Araújo, Fausto H. V.
; Leal, Felipe D. S.
.
Revista Brasileira de Engenharia Agrícola e Ambiental
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RESUMO A destinação final de resíduos sólidos industriais é um dos principais problemas ambientais enfrentados atualmente devido ao seu potencial de contaminação. Com a evolução da política e consciência ambiental, a destinação adequada desses resíduos é estimulada, buscando-se novas tecnologias para suprir a demanda dos produtos gerados. Uma das soluções para esse problema ambiental é a utilização desses resíduos como fertilizantes na agricultura. Objetivou-se com esse trabalho avaliar o uso de composto de resíduos tóxicos da indústria têxtil como componente de substrato de produção de mudas de café e como fertilizante no crescimento inicial de plantas de café. O estudo foi dividido em três experimentos: produção de mudas, crescimento inicial das plantas em vasos e no campo. Na produção de mudas, os tratamentos constaram de diferentes proporções de composto orgânico de resíduo da indústria têxtil: 0, 4, 8, 16 e 32% e um tratamento convencional de produção de mudas. Em vasos e no campo foram utilizados os mesmos tratamentos com composto orgânico e um tratamento convencional de plantio. Nos três experimentos foram realizadas análises de crescimento e nutricional das plantas. O tratamento convencional proporcionou melhor desenvolvimento das mudas e das plantas de café em vasos e em campo, para a maioria dos parâmetros avaliados. Os tratamentos entre 8 e 16% de composto orgânico apresentaram desenvolvimento aceitável das plantas de cafeeiro, demonstrando o seu potencial como fertilizante.
ABSTRACT The final destination of solid industrial waste is one of the main environmental problems currently facing, due to its potential for contamination. With the evolution of environmental policy and awareness, the proper destination of these wastes is stimulated, seeking new technologies to fulfil the demand for the products generated. One of the solutions to this environmental problem is to use these residues as fertilizers in agriculture. The objective of this study was to evaluate the use of toxic textile residues as a substrate component of coffee seedlings and as fertilizer in the initial growth of coffee plants. The study was divided into three experiments: production of seedlings, initial growth of potted plants and in the field. In the production of seedlings, the treatments consisted of different proportions of organic compost residues from the textile industry: 0, 4, 8, 16 and 32% and a conventional treatment of seedling production. In pots and in the field, the same treatments were used with organic compound, and a conventional planting treatment. In the three experiments, plant growth and nutritional analyses were performed. The conventional treatment provided better development of the seedlings and the potted and field coffee plants for most of the evaluated parameters. The treatments between 8 and 16% of organic compost showed an acceptable development of coffee plants, demonstrating their potential as fertilizer.
https://doi.org/10.1590/1807-1929/agriambi.v23n3p188-195
1195 downloads
10.
Growing knowledge: an overview of Seed Plant diversity in Brazil
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Zappi, Daniela C.
; Filardi, Fabiana L. Ranzato
; Leitman, Paula
; Souza, Vinícius C.
; Walter, Bruno M.T.
; Pirani, José R.
; Morim, Marli P.
; Queiroz, Luciano P.
; Cavalcanti, Taciana B.
; Mansano, Vidal F.
; Forzza, Rafaela C.
; Abreu, Maria C.
; Acevedo-Rodríguez, Pedro
; Agra, Maria F.
; Almeida Jr., Eduardo B.
; Almeida, Gracineide S.S.
; Almeida, Rafael F.
; Alves, Flávio M.
; Alves, Marccus
; Alves-Araujo, Anderson
; Amaral, Maria C.E.
; Amorim, André M.
; Amorim, Bruno
; Andrade, Ivanilza M.
; Andreata, Regina H.P.
; Andrino, Caroline O.
; Anunciação, Elisete A.
; Aona, Lidyanne Y.S.
; Aranguren, Yani
; Aranha Filho, João L.M.
; Araújo, Andrea O.
; Araújo, Ariclenes A.M.
; Araújo, Diogo
; Arbo, María M.
; Assis, Leandro
; Assis, Marta C.
; Assunção, Vivian A.
; Athiê-Souza, Sarah M.
; Azevedo, Cecilia O.
; Baitello, João B.
; Barberena, Felipe F.V.A.
; Barbosa, Maria R.V.
; Barros, Fábio
; Barros, Lucas A.V.
; Barros, Michel J.F.
; Baumgratz, José F.A.
; Bernacci, Luis C.
; Berry, Paul E.
; Bigio, Narcísio C.
; Biral, Leonardo
; Bittrich, Volker
; Borges, Rafael A.X.
; Bortoluzzi, Roseli L.C.
; Bove, Cláudia P.
; Bovini, Massimo G.
; Braga, João M.A.
; Braz, Denise M.
; Bringel Jr., João B.A.
; Bruniera, Carla P.
; Buturi, Camila V.
; Cabral, Elza
; Cabral, Fernanda N.
; Caddah, Mayara K.
; Caires, Claudenir S.
; Calazans, Luana S.B.
; Calió, Maria F.
; Camargo, Rodrigo A.
; Campbell, Lisa
; Canto-Dorow, Thais S.
; Carauta, Jorge P.P.
; Cardiel, José M.
; Cardoso, Domingos B.O.S.
; Cardoso, Leandro J.T.
; Carneiro, Camila R.
; Carneiro, Cláudia E.
; Carneiro-Torres, Daniela S.
; Carrijo, Tatiana T.
; Caruzo, Maria B.R.
; Carvalho, Maria L.S.
; Carvalho-Silva, Micheline
; Castello, Ana C.D.
; Cavalheiro, Larissa
; Cervi, Armando C.
; Chacon, Roberta G.
; Chautems, Alain
; Chiavegatto, Berenice
; Chukr, Nádia S.
; Coelho, Alexa A.O.P.
; Coelho, Marcus A.N.
; Coelho, Rubens L.G.
; Cordeiro, Inês
; Cordula, Elizabeth
; Cornejo, Xavier
; Côrtes, Ana L.A.
; Costa, Andrea F.
; Costa, Fabiane N.
; Costa, Jorge A.S.
; Costa, Leila C.
; Costa-e-Silva, Maria B.
; Costa-Lima, James L.
; Cota, Maria R.C.
; Couto, Ricardo S.
; Daly, Douglas C.
; De Stefano, Rodrigo D.
; De Toni, Karen
; Dematteis, Massimiliano
; Dettke, Greta A.
; Di Maio, Fernando R.
; Dórea, Marcos C.
; Duarte, Marília C.
; Dutilh, Julie H.A.
; Dutra, Valquíria F.
; Echternacht, Lívia
; Eggers, Lilian
; Esteves, Gerleni
; Ezcurra, Cecilia
; Falcão Junior, Marcus J.A.
; Feres, Fabíola
; Fernandes, José M.
; Ferreira, D.M.C.
; Ferreira, Fabrício M.
; Ferreira, Gabriel E.
; Ferreira, Priscila P.A.
; Ferreira, Silvana C.
; Ferrucci, Maria S.
; Fiaschi, Pedro
; Filgueiras, Tarciso S.
; Firens, Marcela
; Flores, Andreia S.
; Forero, Enrique
; Forster, Wellington
; Fortuna-Perez, Ana P.
; Fortunato, Reneé H.
; Fraga, Cléudio N.
; França, Flávio
; Francener, Augusto
; Freitas, Joelcio
; Freitas, Maria F.
; Fritsch, Peter W.
; Furtado, Samyra G.
; Gaglioti, André L.
; Garcia, Flávia C.P.
; Germano Filho, Pedro
; Giacomin, Leandro
; Gil, André S.B.
; Giulietti, Ana M.
; A.P.Godoy, Silvana
; Goldenberg, Renato
; Gomes da Costa, Géssica A.
; Gomes, Mário
; Gomes-Klein, Vera L.
; Gonçalves, Eduardo Gomes
; Graham, Shirley
; Groppo, Milton
; Guedes, Juliana S.
; Guimarães, Leonardo R.S.
; Guimarães, Paulo J.F.
; Guimarães, Elsie F.
; Gutierrez, Raul
; Harley, Raymond
; Hassemer, Gustavo
; Hattori, Eric K.O.
; Hefler, Sonia M.
; Heiden, Gustavo
; Henderson, Andrew
; Hensold, Nancy
; Hiepko, Paul
; Holanda, Ana S.S.
; Iganci, João R.V.
; Imig, Daniela C.
; Indriunas, Alexandre
; Jacques, Eliane L.
; Jardim, Jomar G.
; Kamer, Hiltje M.
; Kameyama, Cíntia
; Kinoshita, Luiza S.
; Kirizawa, Mizué
; Klitgaard, Bente B.
; Koch, Ingrid
; Koschnitzke, Cristiana
; Krauss, Nathália P.
; Kriebel, Ricardo
; Kuntz, Juliana
; Larocca, João
; Leal, Eduardo S.
; Lewis, Gwilym P.
; Lima, Carla T.
; Lima, Haroldo C.
; Lima, Itamar B.
; Lima, Laíce F.G.
; Lima, Laura C.P.
; Lima, Leticia R.
; Lima, Luís F.P.
; Lima, Rita B.
; Lírio, Elton J.
; Liro, Renata M.
; Lleras, Eduardo
; Lobão, Adriana
; Loeuille, Benoit
; Lohmann, Lúcia G.
; Loiola, Maria I.B.
; Lombardi, Julio A.
; Longhi-Wagner, Hilda M.
; Lopes, Rosana C.
; Lorencini, Tiago S.
; Louzada, Rafael B.
; Lovo, Juliana
; Lozano, Eduardo D.
; Lucas, Eve
; Ludtke, Raquel
; Luz, Christian L.
; Maas, Paul
; Machado, Anderson F.P.
; Macias, Leila
; Maciel, Jefferson R.
; Magenta, Mara A.G.
; Mamede, Maria C.H.
; Manoel, Evelin A.
; Marchioretto, Maria S.
; Marques, Juliana S.
; Marquete, Nilda
; Marquete, Ronaldo
; Martinelli, Gustavo
; Martins da Silva, Regina C.V.
; Martins, Ângela B.
; Martins, Erika R.
; Martins, Márcio L.L.
; Martins, Milena V.
; Martins, Renata C.
; Matias, Ligia Q.
; Maya-L., Carlos A.
; Mayo, Simon
; Mazine, Fiorella
; Medeiros, Debora
; Medeiros, Erika S.
; Medeiros, Herison
; Medeiros, João D.
; Meireles, José E.
; Mello-Silva, Renato
; Melo, Aline
; Melo, André L.
; Melo, Efigênia
; Melo, José I.M.
; Menezes, Cristine G.
; Menini Neto, Luiz
; Mentz, Lilian A.
; Mezzonato, A.C.
; Michelangeli, Fabián A.
; Milward-de-Azevedo, Michaele A.
; Miotto, Silvia T.S.
; Miranda, Vitor F.O.
; Mondin, Cláudio A.
; Monge, Marcelo
; Monteiro, Daniele
; Monteiro, Raquel F.
; Moraes, Marta D.
; Moraes, Pedro L.R.
; Mori, Scott A.
; Mota, Aline C.
; Mota, Nara F.O.
; Moura, Tania M.
; Mulgura, Maria
; Nakajima, Jimi N.
; Nardy, Camila
; Nascimento Júnior, José E.
; Noblick, Larry
; Nunes, Teonildes S.
; O'Leary, Nataly
; Oliveira, Arline S.
; Oliveira, Caetano T.
; Oliveira, Juliana A.
; Oliveira, Luciana S.D.
; Oliveira, Maria L.A.A.
; Oliveira, Regina C.
; Oliveira, Renata S.
; Oliveira, Reyjane P.
; Paixão-Souza, Bruno
; Parra, Lara R.
; Pasini, Eduardo
; Pastore, José F.B.
; Pastore, Mayara
; Paula-Souza, Juliana
; Pederneiras, Leandro C.
; Peixoto, Ariane L.
; Pelissari, Gisela
; Pellegrini, Marco O.O.
; Pennington, Toby
; Perdiz, Ricardo O.
; Pereira, Anna C.M.
; Pereira, Maria S.
; Pereira, Rodrigo A.S.
; Pessoa, Clenia
; Pessoa, Edlley M.
; Pessoa, Maria C.R.
; Pinto, Luiz J.S.
; Pinto, Rafael B.
; Pontes, Tiago A.
; Prance, Ghillean T.
; Proença, Carolyn
; Profice, Sheila R.
; Pscheidt, Allan C.
; Queiroz, George A.
; Queiroz, Rubens T.
; Quinet, Alexandre
; Rainer, Heimo
; Ramos, Eliana
; Rando, Juliana G.
; Rapini, Alessandro
; Reginato, Marcelo
; Reis, Ilka P.
; Reis, Priscila A.
; Ribeiro, André R.O.
; Ribeiro, José E.L.S.
; Riina, Ricarda
; Ritter, Mara R.
; Rivadavia, Fernando
; Rocha, Antônio E.S.
; Rocha, Maria J.R.
; Rodrigues, Izabella M.C.
; Rodrigues, Karina F.
; Rodrigues, Rodrigo S.
; Rodrigues, Rodrigo S.
; Rodrigues, Vinícius T.
; Rodrigues, William
; Romaniuc Neto, Sérgio
; Romão, Gerson O.
; Romero, Rosana
; Roque, Nádia
; Rosa, Patrícia
; Rossi, Lúcia
; Sá, Cyl F.C.
; Saavedra, Mariana M.
; Saka, Mariana
; Sakuragui, Cássia M.
; Salas, Roberto M.
; Sales, Margareth F.
; Salimena, Fatima R.G.
; Sampaio, Daniela
; Sancho, Gisela
; Sano, Paulo T.
; Santos, Alessandra
; Santos, Élide P.
; Santos, Juliana S.
; Santos, Marianna R.
; Santos-Gonçalves, Ana P.
; Santos-Silva, Fernanda
; São-Mateus, Wallace
; Saraiva, Deisy P.
; Saridakis, Dennis P.
; Sartori, Ângela L.B.
; Scalon, Viviane R.
; Schneider, Ângelo
; Sebastiani, Renata
; Secco, Ricardo S.
; Senna, Luisa
; Senna-Valle, Luci
; Shirasuna, Regina T.
; Silva Filho, Pedro J.S.
; Silva, Anádria S.
; Silva, Christian
; Silva, Genilson A.R.
; Silva, Gisele O.
; Silva, Márcia C.R.
; Silva, Marcos J.
; Silva, Marcos J.
; Silva, Otávio L.M.
; Silva, Rafaela A.P.
; Silva, Saura R.
; Silva, Tania R.S.
; Silva-Gonçalves, Kelly C.
; Silva-Luz, Cíntia L.
; Simão-Bianchini, Rosângela
; Simões, André O.
; Simpson, Beryl
; Siniscalchi, Carolina M.
; Siqueira Filho, José A.
; Siqueira, Carlos E.
; Siqueira, Josafá C.
; Smith, Nathan P.
; Snak, Cristiane
; Soares Neto, Raimundo L.
; Soares, Kelen P.
; Soares, Marcos V.B.
; Soares, Maria L.
; Soares, Polyana N.
; Sobral, Marcos
; Sodré, Rodolfo C.
; Somner, Genise V.
; Sothers, Cynthia A.
; Sousa, Danilo J.L.
; Souza, Elnatan B.
; Souza, Élvia R.
; Souza, Marcelo
; Souza, Maria L.D.R.
; Souza-Buturi, Fátima O.
; Spina, Andréa P.
; Stapf, María N.S.
; Stefano, Marina V.
; Stehmann, João R.
; Steinmann, Victor
; Takeuchi, Cátia
; Taylor, Charlotte M.
; Taylor, Nigel P.
; Teles, Aristônio M.
; Temponi, Lívia G.
; Terra-Araujo, Mário H.
; Thode, Veronica
; Thomas, W.Wayt
; Tissot-Squalli, Mara L.
; Torke, Benjamin M.
; Torres, Roseli B.
; Tozzi, Ana M.G.A.
; Trad, Rafaela J.
; Trevisan, Rafael
; Trovó, Marcelo
; Valls, José F.M.
; Vaz, Angela M.S.F.
; Versieux, Leonardo
; Viana, Pedro L.
; Vianna Filho, Marcelo D.M.
; Vieira, Ana O.S.
; Vieira, Diego D.
; Vignoli-Silva, Márcia
; Vilar, Thaisa
; Vinhos, Franklin
; Wallnöfer, Bruno
; Wanderley, Maria G.L.
; Wasshausen, Dieter
; Watanabe, Maurício T.C.
; Weigend, Maximilian
; Welker, Cassiano A.D.
; Woodgyer, Elizabeth
; Xifreda, Cecilia C.
; Yamamoto, Kikyo
; Zanin, Ana
; Zenni, Rafael D.
; Zickel, Carmem S
.
Resumo Um levantamento atualizado das plantas com sementes e análises relevantes acerca desta biodiversidade são apresentados. Este trabalho se iniciou em 2010 com a publicação do Catálogo de Plantas e Fungos e, desde então vem sendo atualizado por mais de 430 especialistas trabalhando online. O Brasil abriga atualmente 32.086 espécies nativas de Angiospermas e 23 espécies nativas de Gimnospermas e estes novos dados mostram um aumento de 3% da riqueza em relação a 2010. A Amazônia é o Domínio Fitogeográfico com o maior número de espécies de Gimnospermas, enquanto que a Floresta Atlântica possui a maior riqueza de Angiospermas. Houve um crescimento considerável no número de espécies e nas taxas de endemismo para a maioria dos Domínios (Caatinga, Cerrado, Floresta Atlântica, Pampa e Pantanal), com exceção da Amazônia que apresentou uma diminuição de 2,5% de endemicidade. Entretanto, a maior parte das plantas com sementes que ocorrem no Brasil (57,4%) é endêmica deste território. A proporção de formas de vida varia de acordo com os diferentes Domínios: árvores são mais expressivas na Amazônia e Floresta Atlântica do que nos outros biomas, ervas são dominantes no Pampa e as lianas apresentam riqueza expressiva na Amazônia, Floresta Atlântica e Pantanal. Este trabalho não só quantifica a biodiversidade brasileira, mas também indica as lacunas de conhecimento e o desafio a ser enfrentado para a conservação desta flora.
Abstract An updated inventory of Brazilian seed plants is presented and offers important insights into the country's biodiversity. This work started in 2010, with the publication of the Plants and Fungi Catalogue, and has been updated since by more than 430 specialists working online. Brazil is home to 32,086 native Angiosperms and 23 native Gymnosperms, showing an increase of 3% in its species richness in relation to 2010. The Amazon Rainforest is the richest Brazilian biome for Gymnosperms, while the Atlantic Rainforest is the richest one for Angiosperms. There was a considerable increment in the number of species and endemism rates for biomes, except for the Amazon that showed a decrease of 2.5% of recorded endemics. However, well over half of Brazillian seed plant species (57.4%) is endemic to this territory. The proportion of life-forms varies among different biomes: trees are more expressive in the Amazon and Atlantic Rainforest biomes while herbs predominate in the Pampa, and lianas are more expressive in the Amazon, Atlantic Rainforest, and Pantanal. This compilation serves not only to quantify Brazilian biodiversity, but also to highlight areas where there information is lacking and to provide a framework for the challenge faced in conserving Brazil's unique and diverse flora.
https://doi.org/10.1590/2175-7860201566411
33340 downloads
11.
Seedlings of Acrocomia aculeata in diferent substrates and protected environments
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Costa, Edilson
; Martins, Roberta F.
; Faria, Tatiane A. C.
; Jorge, Marçal H. A.
; Leal, Paulo A. M.
.
A etapa de produção de mudas é a fase fundamental para obtenção de uniformidade no plantel. Este trabalho avaliou a formação de mudas de bocaiúva (Acrocomia aculeata), com sementes pré-geminadas, em diferentes substratos e ambientes protegidos, na Universidade Estadual de Mato Grosso do Sul, Aquidauana-MS. Como substratos, foram utilizados 100% de esterco bovino, 100% de ramas de mandioca, 100% de vermiculita, 50% de esterco bovino + 50% de ramas de mandioca, 50% de esterco bovino + 50% de vermiculita, 50% de ramas de mandioca + 50% de vermiculita e ⅓ de esterco bovino + ⅓ de ramas de mandioca + ⅓ de vermiculita. Estes substratos foram testados na estufa agrícola coberta com filme de polietileno de baixa densidade (PEBD) de 150 µm, com tela termorrefletora de 50% de sombreamento sob o filme e tela preta de 50% de sombreamento nas laterais, no telado com tela preta de mono filamento de 50% de sombreamento na cobertura e nas laterais, e no telado com tela aluminizada termorrefletora de 50% de sombreamento na cobertura e nas laterais. Foi adotado o delineamento experimental inteiramente casualizado, com 5 repetições de 5 plantas cada. Inicialmente, os dados foram submetidos às análises de variâncias individuais dos substratos, em cada ambiente de cultivo, realizando em seguida a avaliação dos quadrados médios dos resíduos e a análise conjunta dos ambientes, para comparação destes. O melhor ambiente de cultivo é o de tela termorrefletora, comparado à estufa de PEBD e ao telado preto. Todos os substratos que contêm esterco bovino são indicados para a formação de mudas de bocaiúva. A rama de mandioca pura não é indicada para formação de mudas, mesmo utilizando adubação química.
The seedling production stage is the key to achieve uniformity in tree breeding stage. This study evaluated "bocaiúva" (Acrocomia aculeata) seedling formation, with pre-germinated seeds in different substrates and protected environments, in the University of Mato Grosso do Sul State, Aquidauana, MS. As substrates, we used 100% cattle manure (M), 100% cassava branches (CB), 100% vermiculite (V), 50% cattle manure + 50% cassava branches, 50 % cattle manure + 50% vermiculite, 50% cassava branches + 50% vermiculite and ⅓ cattle manure + ⅓ cassava branches + ⅓ vermiculite. These substrates were tested in a greenhouse covered with 150 µm low density polyethylene (LDPE) film under thermo-reflective screen with 50% shading under film; black screen with 50% shading on the sides; black monofilament screen with 50% shading set on roof and sides; and aluminized thermo- reflective screen with 50% shading set on roof and sides. The completely randomized experimental design with 5 replications of 5 plants each was adopted. Initially, data were submitted to analysis of substrate individual variance in each growing environment, then performing the waste mean square evaluation and their environment joint analysis for comparison. The best growing environment is the thermo-reflective screen compared to LDPE greenhouse and black screen set. All substrates containing manure are recommended for bocaiúva seedlings formation. The pure cassava branch is not indicated for seedling, even using chemical fertilizer.
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12.
Ecomorphology of Astyanax species in streams with different substrates
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In the present study, we assessed the ecomorphology of two species of Astyanax in streams with different substrates found in the Rio São Francisco Basin. The dominant substrate of each stream was defined as either "fine" (0 to 2 mm), "gravel" (2 to 250 mm), "rock" (> 250 mm), or "leaf bank". We analyzed a total of 22 ecomorphological attributes of Astyanax intermedius Eigenmann, 1908 (127 individuals) and Astyanax rivularis (Lütken, 1875) (238 individuals) adults. We detected significant ecomorphological differences between the populations of A. rivularis and A. intermedius from habitats with different types of substrates. However, the two species did not show the same morphological differences depending on the type of substrate. These results confirmed the hypothesis that individuals from environments with different characteristics may have different ecomorphological patterns. Knowing that morphology is associated with habitat use and available resources, the loss of a resource or a modification in the environment may directly affect the permanence of a species, leading to a loss of morphologic diversity.
https://doi.org/10.1590/S1984-46702014000100006
3009 downloads
13.
Transdermal formulations containing human sexual steroids: development and validation of methods and in vitro drug release
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Polonini, Hudson C.
; Soldati, Pedro Paulo
; Oliveira, Marcone Augusto Leal de
; Brandão, Marcos Antônio F.
; Chaves, Maria das Graças M.
; Raposo, Nádia R. B.
.
In vitro release of bioidentical hormones in four different liposomal transdermal emulsions (containing testosterone, progesterone, estradiol, or estradiol and estriol) was assessed. For this purpose, novel high-performance liquid chromatography methods were developed and validated in an eco-friendly manner and used to determine the in vitro release of such products. The methods were suitable for our intended goal, and the emulsions employed were found to be effective as transporting candidates for the efficient release of hormones in the transdermal delivery of human sexual steroids.
https://doi.org/10.5935/0100-4042.20140116
2600 downloads
14.
Protozooplankton and its relationship with environmental conditions in 13 water bodies of the Mogi-Guaçu basin - SP, Brazil
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Bagatini, Inessa Lacativa
; Spínola, Ana Lúcia Gerardi
; Peres, Bianca de Miranda
; Mansano, Adrislaine da Silva
; Rodrigues, Mafalda Alexandra Antunes
; Batalha, Marco Antônio Portugal Luttembarck
; Lucca, José Valdecir de
; Godinho, Mirna Januária Leal
; Tundisi, Takako Matsumura
; Seleghim, Mirna Helena Regali
.
O protozooplâncton é um componente importante da rede trófica microbiana de ambientes aquáticos e sua composição, densidade e distribuição refletem os aspectos físicos, químicos e bióticos do ambiente. Considerando a escassa literatura sobre protozoários de água doce no Brasil e sobre sua ecologia em ambientes subtropicais, inventariamos os táxons de ciliados e amebas em 13 corpos d'água do Estado de São Paulo e analisamos a variação na abundância dos gêneros/espécies de maior incidência em relação às variáveis ambientais. Coletamos duas amostras por ambiente, fixando-as com cloreto de mercúrio e corando-as com azul de bromofenol para posterior quantificação e identificação em microscópio ótico. Identificamos 74 gêneros de ciliados e amebas, e os Ciliophora dominaram na maioria dos ambientes. A subclasse Stichotrichia ocorreu em todos os ambientes, predominando em cinco deles, especialmente pela ocorrência o gênero Halteria. A Análise de Correspondência Canônica mostrou que as concentrações de nitrito e nitrato são as principais variáveis que explicam a distribuição dos gêneros Limnostrombidium, Urotricha e Vorticella. O aumento na concentração de oxigênio dissolvido, condutividade, temperatura e concentração de fosfato particulado afetou positivamente a densidade dos gêneros Halteria e Coleps e da espécie Cinetochilum margaritaceum, que foi ainda influenciada negativamente pelo aumento nas concentrações de nitrito e nitrato. Considerando-se que foi realizada apenas uma coleta, a riqueza de espécies foi alta quando comparada à média de taxa encontrada para corpos d'água do Estado de São Paulo. O ambiente mais rico, Lagoa do Diogo, localiza-se em uma estação ecológica, confirmando a necessidade de mais pesquisas sobre a diversidade em ambientes menos impactados.
Protozooplankton is an important component of the aquatic microbial food webs and its composition, density, and distribution reflect the chemical, physical, and biological aspects of the environment. Considering the scarce literature on freshwater protozoans in Brazil and on protozoan ecology in subtropical environments, we listed the ciliates and amoebae taxa found in 13 water bodies in São Paulo State and analyzed their abundance in relation to the environmental variables. We collected two samples in each environment, fixed immediately with mercuric chloride and stained with bromophenol blue. After microscopical analysis, 74 protozoan genera were identified and the Ciliophora were dominant in the majority of the environments. The Stichotrichia, represented mostly by the genus Halteria, occurred in all environments, and was the dominant subclass in five of them. The canonic correspondence analysis of the most frequent genera and the environmental variables showed that nitrite and nitrate were the variables that better explained the distribution of Limnostrombidium, Urotricha, and Vorticella. The densities of the genera Halteria, Coleps, and of the species Cinetochilum margaritaceum were positively affected by increasing concentrations of dissolved oxygen, particulate phosphate, conductivity, and temperature. C. margaritaceum were also negatively affected by increasing concentrations of nitrite and nitrate. Considering that we made only one sampling in each environment, the richness was high compared to the mean diversity of lakes in the São Paulo State. The Diogo Lake, located in an ecological reserve, was the richest one, confirming the need of more research on the biodiversity of more preserved environments.
https://doi.org/10.1590/S1676-06032013000400016
2788 downloads
15.
Production of tomato seedlings using different substrates and trays in three protected environments
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Costa, Edilson
; Leal, Paulo A. M.
; Benett, Cleiton G. S.
; Benett, Katiane S. S.
; Salamene, Luiz C. P.
.
A produção de mudas é uma etapa essencial na cadeia produtiva de hortaliças. Desta forma, este trabalho teve os objetivos de avaliar a ambiência, os substratos e os recipientes no desenvolvimento de mudas de tomateiro, cv. Santa Cruz Kada Gigante, na região de Aquidauana-MS, de outubro a novembro de 2008. Foram testadas bandejas de poliestireno com 72; 128 e 200 células, preenchidas com quatro substratos: solo; Plantmax®; fibra de coco fina, e vermiculita, em três ambientes protegidos: estufa, telado com Sombrite® e telado com Aluminet®. O experimento foi conduzido em delineamento inteiramente casualizado, em esquema fatorial 3 x 4 (três bandejas x quatro substratos), com quatro repetições, realizando-se de análises de variâncias individuais e análise conjunta dos ambientes. Os ambientes telados (Sombrite® e Aluminet®), a bandeja com 72 células e a vermiculita produziram as melhores mudas.
The seedlings production is an essential part for vegetables production. Thus, this study aimed to evaluate the environment, the substrates and the containers in the development of tomato seedlings, cv. Santa crus Kada Gigante, in Aquidauana -MS, Brazil region, from October to November, 2008. Polystyrene trays with 72; 128 and 200 cells, filled with four substrates (soil; Plantmax®; coconut fiber and vermiculite) were tested in three protected environments (greenhouse; screened with Sombrite® and screened with Aluminet®). The experimental design was completely randomized, factorial scheme 3x4 (three trays x four substrates), with four replications, being analyzed individual variance analysis and joint analysis for the environments. The environment with screens (Sombrite® and Aluminet®), the trays with 72 cells and the vermiculite produced better results.
3085 downloads
Cited 2 times in SciELO
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