ABSTRACT The various types of lignocellulosic biomass found in plants comprise the most abundant renewable bioresources on Earth. In this study, the ruminal microbial ecosystem of black goats was explored because of their strong ability to digest lignocellulosic forage. A metagenomic fosmid library containing 115,200 clones was prepared from the black-goat rumen and screened for a novel cellulolytic enzyme. The KG35 gene, containing a novel glycosyl hydrolase family 5 cellulase domain, was isolated and functionally characterized. The novel glycosyl hydrolase family 5 cellulase gene is composed of a 963-bp open reading frame encoding a protein of 320 amino acid residues (35.1 kDa). The deduced amino acid sequence showed the highest sequence identity (58%) for sequences from the glycosyl hydrolase family 5 cellulases. The novel glycosyl hydrolase family 5 cellulase gene was overexpressed in Escherichia coli. Substrate specificity analysis revealed that this recombinant glycosyl hydrolase family 5 cellulase functions as an endo-β-1,4-glucanase. The recombinant KG35 endo-β-1,4-glucanase showed optimal activity within the range of 30-50 °C at a pH of 6-7. The thermostability was retained and the pH was stable in the range of 30-50 °C at a pH of 5-7.

The present research paper deals with the oxidation process of molybdenum ore. The main target of the present study is the up-gradation of MoO3 from roasted molybdenum ore by a leaching process without waste generation. The most important application of hydrometallurgical processing is the leaching process of the ore and it is the primary process to make pure metal from ore. The present investigations optimize the following experimental parameters to improve the concentration of MoO3 as well as the separation of copper, iron and zinc in roasted molybdenum ore: effect of acid concentration, temperature, pulp density and leaching time were studied systematically. The temperature study was carried out at 550-595 ºC for the oxidation process. The XRD result shows that oxidation process of molybdenum ore and SEM pictures were taken for particles before and after the oxidation process at 585 ºC for 360 min.

Neste estudo, extratores à base de amina (Alamine 336, Alamine 308, Alamine 304 e Aliquat 336) diluídos em querosene foram usados para extração de urânio e separação de outros elementos associados. Alamine 336 foi o melhor extrator para o processo de extração de urânio de soluções de sulfato quando comparado com outros reagentes à base de amina, Alamine 308, 304 Alamine 336 e Aliquat 336. O comportamento da extração sinérgica foi estudado com aminas como extratantes assim como reagentes sinergistas e organofosforados usados em misturas sinergistas com aminas. Os estudos de extração sinérgica com aminas não foram adequados entre si para melhor eficiência da extração. No entanto, aminas misturadas com extratores organofosforados forneceram comportamento sinérgico positivo, com um coeficiente de 0,567, calculado a partir de resultados obtidos. A metodologia desenvolvida foi aplicada ao processamento de minério de baixo grau de urânio, e 99,83% de urânio foi recuperado sem interferências de outros metais.

In this study, amine based extractants (Alamine 336, Alamine 308, Alamine 304 and Aliquat 336) diluted in kerosene were used as promising extractants for uranium extraction and separation from other associated elements. Alamine 336 was the best extractant for uranium extraction process from sulfate solutions when compared with other amine based extractants, Alamine 308, Alamine 304 and Aliquat 336. Synergistic extraction behavior was studied with amines as extractants as well as synergists and organophosphorus reagents used as synergist mixed with amines. Synergistic extraction studies with amines were not suitable with each other for better extraction efficiency. However, amines mixed with organophosphorus extractants gave positive synergetic behavior with the highest synergistic coefficient 0.567 calculated from results obtained. The developed methodology was applied to uranium low grade ore processing and 99.83% of uranium was recovered without the interferences of other metals.