Células do baço de camundongos foram examinadas aos 5, 10, 20 e 25 dias pós-infecção (dpi) com Dermatobia hominis e examinadas aos 5, 10, 15, 30 e 60 dias pós-emergência da larva (dpel). As células foram cultivadas em meio RPMI-1640 contendo, ou não (controle), produtos de secreção das larvas (PSL) de D. hominis com idade de 5, 10, 15, 20 e 25 dias. Em cada grupo com cinco camundongos testados nos meios de cultura, o número de células foi significativo para 25 dpi, com crescente aumento na seguinte ordem: PSL-10 d, -25 d, -5 d, -20 d, -15 d e RPMI. Resultados significantes foram também observados nos testes entre cada meio contendo células tanto de camundongos dpi ou dpel. Em cada dpel grupo versus meio significância foi somente verificada para 10 dpel, na ordem crescente: PSL-5 d, -20 d, -25 d, -10 d, -15 d e RPMI. Testes comparativos foram também realizados entre grupos. O PSL foi analisado sob SDS-PAGE. Os resultados provam que a miíase causou depleção de células do baço, particularmente sob efeito do PSL-10 e -15, mas ocorreu normalidade do número de células aos 60 dpel. Este ensaio in vitro pode representar uma resposta imune sistêmica na relação PSL-D. hominis-hospedeiro.

Spleen cells from mice were examined at 5, 10, 15, 20 and 25 days post-infection (dpi) with Dermatobia hominis larva and at 5, 10, 15, 30 and 60 days post-larval emergence (dple). Cell proliferation in vitro assays were carried out with RPMI-1640 medium and larval secretory product (LSP) of D. hominis at 5, 10, 15, 20 and 25 days. When each group of mice was tested against each medium, significance was only seen for 25 dpi, with increasing order: LSP-10 d, -25 d, -5 d, -20 d, -15 d and RPMI. Significant results were also observed when each medium was tested against mice at each dpi or dple. Each dple group vs. each medium produced significant results only for 10 dple, with increasing order: LSP-5 d, -20 d, -25 d, -10 d, -15 d and RPMI. Comparative tests were also carried out between groups to refine certain observations. The LSPs were also analyzed using SDS-PAGE. The results prove that myiasis caused depletion of spleen cells, particularly under the effect of the LSP-10 and -15, but the cells tended to increase up to 60 dple. This in vitro assay may represent the real systemic immune response in the relationship LSP-D. hominis-host.

The second and third instar larvae, and the pupa of Metacuterebra apicalis (Guérin-Menevilli), are described based on light and scanning electron microscope observations

The morphology and chaetotaxy of the first instar larva of Lutzomyia (Lutzomyia) longipalpis are described based on observations made under scanning electron microscope. Because three-dimensional images were studied, some terminological changes are proposed to give a more realistic description of the positions of the setae. On the larval body, the pairs of setae have the following number: 9 on the head, 12 on the prothorax, 8 on the meso- and metathorax, 6 on the first to eighth abdominal segments, and 8 on the ninth abdominal segment

The fourth instar larva of Lutzomyia (Lutzomyia) longipalpis (Phlebotomidae: Phlebotominae) was studied by scanning electron microscope. Based on three-dimensional observations, the fine structure and setal position (using of setal numeration) of the larva are presented.

Cochliomyia hominivorax and C. macellaria are species that cause primary and secondary myiasis, respectively of medical and veterinary importance in the New World. The male terminalia of both insects show morphological differences that are now described from scanning electron microscope (SEM) observations.

The eggs of Muscina stabulans and Synthesiomyia nudiseta are morphologically described, based on scanning electron microscope (SEM).

The morphology of the male and female of Bunostomum phlebotomum are described based on scanning electron microscope (SEM) observations. The attachment of the worms to the small intestinal mucosa and during the copula were observed. Structures of the bucal capsule and genital organs were also studied.

Based on scanning electron microscope (SEM) estudies, the male genitalia of three species of Ravinia and two species of Chaetoravinia (Diptera: Sarcophagidae, subtribe Raviniina) are described and compared.

The male genitalia of nine species of Sarcophagidae (Diptera) - Goniophyto honsuensis Rohdendorf, 1962, Tricharaea brevicornis (Wiedemann, 1830), Chaetoravinia derelicta (Walker, 1852), Austrohartigia spinigena (Rondani, 1864), Chrysagria duodecimpunctata Townsend, 1935, Boettcheria bisetosa Parker, 1914, Lipoptilocnema lanei Townsend, 1934, L. crispina (Lopes, 1938) and Euboettcheria alvarengai Lopes & Tibana, 1982 - were examined by scanning electron microscope (SEM) and the main morphological features are descirbed.

O ovo de Sarcodexia lambens (Diptera: Sarcophagidae) é descrito a nível de microscopia eletrônica de varredura.

The egg of Sarcodexia lambens (Diptera: Sarcophagidae) is described on the basis of scanning electron microscopy.

As genitálias dos machos de Oxyvinia excisa (Lopes), Oxysarcodexia tornax (Walker), O. fluminensis Lopes, sarcodexia lambens (Wiedemann), Peckia chrysostoma (Wiedemann) e Liopygia ruficornis (Fabricius) foram estudadas com base em fotografias de microscópio de varredura. Alguns detalhes importantes foram evidenciados por este método.

Male genitalia of Oxyvinia exicisa (Lopes), Oxysarcodexia thomax (Walker), O. fluminensis Lopes, Sarcodexia lambens (Wiedemann), Peckia chrysostoma (Wiedemann) and Liopygia ruficornis (Fabricius) were studied based on scanning electron microscope photography. Some important details were evidentiated with this method.

O ovo e a larva de primeiro estágio de Metacuterebra apicalis (Diptera, Cuterebridae) são descritos a nível de microscopia óptica e eletrônica de varredura.

Descriptions are given of the egg and first intar larvar of Metacutereba apicalis (Diptera: Cuterebridae) when viewed by light and scanning electronic microscopes.

The first instar larvae of Sarcodexia lambens (Wiedemann, 1830) and Peckia chrysostoma (Wiedemann, 1830) dissected from females reared in laboratory, are described under scanning electron microscope (SEM).

Em condições experimentais de laboratório, foi desenvolvido o ciclo biológico de Metacuterebra apicalis. Serviram como hospedeiros Rodentia Oryzomys subflavus e Rattus norvegicus. O ciclo foi desenvolvido em cerca de 73 dias, sendo os períodos aproximados de 10 dias para embrionia dos ovos, de 23 e 26 dias, respectivamente, para o parasitismo em O. sublflavus e R. norvegicus, de 32 dias para pupa, e de seis dias para sobrevida dos insetos adultos.

The development of Metacuterebra apicalis in laboratory conditions is described. The natural host, Oryzomys subflavus, and laboratory white rats were used as experimental hosts. The life cycle, from oviposition to the deaths of adults, was completed in about 73 days. The incubation period of eggs was about 10 days; the parasitic larval phase lasted 23 days in the natural host and 26 days in white rats; pupa lived for 32 days and adults survived for six days.

A redescrição morfológica de Metacuterebra apicalis (Diptera, Cuterebridae) foi usada a nível de microscopia óptica e eletrônica de varredura.

The adults of Metacuterebra apicalis are redescribed on the basis of light and scanning electron microscopy.