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au:LOPES, CELIO M.

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O POTENCIAL DA XANTONA NATURAL α-MANGOSTINA NO DESENVOLVIMENTO DE NOVOS AGENTES ANTI-INFECCIOSOS: UMA REVISÃO αMANGOSTINA α MANGOSTINA ANTIINFECCIOSOS ANTI INFECCIOSOS ANTI-INFECCIOSOS
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Pinto, Douglas Chaves de Alcântara

; Souza, Gabriela Alves de

; Pitasse-Santos, Paulo

; Velez, Afonso Santine M. M.

; Decote-Ricardo, Debora

; Santos, Debora Regina Lopes dos

; Freire-de-Lima, Leonardo

; Freire-de-Lima, Célio G.

; Lima, Marco Edilson Freire de

Química Nova

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2023, Volume 46 N. 1 Pages 77 - 94

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

The mangosteen (Garcinia mangostana, Linn.) is a tropical fruit cultivated in the tropical forests of Southeast Asian countries. It is recognized as the queen of fruits due to its unique color, aroma, and flavor. It has been used for centuries in Southeastern Asian traditional medicine for the treatment of various diseases. Many functional molecules with high medicinal value are obtained from the pericarp of mangosteen fruit, whose major constituent is xanthone α-mangostin. Several studies have been carried out over the last decades to further investigate and understand the pharmacological properties associated with the plant and its main chemical constituents, belonging to the class of xanthones. In addition, numerous in vitro and in vivo studies related to mangosteen have been published, indicating its importance and its potential application in medicinal chemistry. Prenylated xanthones, which include α-mangostin, are secondary metabolites particularly common in plants belonging to the Clusiaceae family. These substances have been intensively investigated for their potential as biologically active agents. This article describes a critical analysis of published data related to the properties of α-mangostin, aiming to provide a more detailed view of its potential as a chemotherapeutic agent for the prevention and treatment of infectious diseases. Garcinia mangostana Linn. Linn countries color aroma flavor diseases αmangostin. αmangostin α mangostin. mangostin α-mangostin constituents xanthones addition chemistry αmangostin, mangostin, family agents

https://doi.org/10.21577/0100-4042.20170954

From genes to ecosystems: a synthesis of amphibian biodiversity research in Brazil
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Haddad, Célio F.B.

; Lopes, Carla M.

; Becker, C. Guilherme

; da Silva, Fernando R.

; Lyra, Mariana L.

Biota Neotropica

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2022, Volume 22 N. spe elocation e20221375

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo No presente estudo apresentamos exemplos de avanços significativos nas pesquisas com anfíbios financiadas pela Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), focando em descobertas recentes nos campos de ecologia de comunidades, modificação do habitat, doenças infecciosas e o sequenciamento de DNA com múltiplos propósitos. Demonstramos que a FAPESP tem sido fundamental não somente pelo financiamento direto de projetos de pesquisa e bolsas de estudo, mas também através de sua política de formação científica, fomentando colaborações internacionais com instituições de pesquisa de excelência mundial, melhorando e consolidando novas linhas de pesquisa que frequentemente dependem da combinação sinérgica entre diferentes linhas de conhecimento e ferramentas complexas. Enfatizamos que futuros estudos continuem com foco em questões básicas, como a descrição de novas espécies, bem como correções taxonômicas e sistemáticas. Além disso, esperamos uma forte integração entre diferentes disciplinas usando novas ferramentas de bioinformática e abordagens de modelagem, como o aprendizado de máquina. Essas novas abordagens serão críticas para desenvolver ainda mais nossa compreensão a respeito de questões fundamentais sobre as características da história de vida dos anfíbios, transmissão de doenças, estrutura de comunidades, biogeografia e previsões populacionais em diferentes cenários de mudanças globais, como a expansão da agricultura, uso de agrotóxicos, perda de habitat e mudanças climáticas.

Abstract Here, we summarize examples of significant advances in amphibian research supported by the São Paulo Research Foundation (FAPESP), focusing on recent discoveries in the fields of community ecology, habitat change, infection diseases, and multipurpose DNA sequencing. We demonstrated that FAPESP has been fundamental not only by directly funding research projects and scholarships, but also through its science training policy, fostering international collaborations with world-class research institutions, improving and consolidating new lines of research that often depended on a synergetic combination of different knowledge and complex tools. We emphasized that future studies will continue to focus on basic questions, such as description of new species, as well as taxonomic and systematic corrections. Furthermore, we also expect that there will be a strong integration among different disciplines using novel bioinformatics tools and modeling approaches, such as machine learning. These new approaches will be critical to further develop our understanding of foundational questions of amphibian life-history trait variation, disease transmission, community assembly, biogeography, and population forecasts under different global change scenarios such as agricultural expansion, agrochemical use, habitat loss, and climate change.

https://doi.org/10.1590/1676-0611-bn-2022-1375

The role of terrestrial bromeliads in determining the spatial organization of plant life forms in a tropical coastal forest
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Lopes, Celio M.

; Misaki, Flora

; Santos, Karina

; Evangelista, Carlos A. P.

; Carrijo, Tatiana T.

; Garbin, Mário L.

Acta Botanica Brasilica

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mar 2017, Volume 31 N. 1 Pages 84 - 92

Abstract: En | Text: En | PDF: En

ABSTRACT The interplay between plant-plant interactions and light heterogeneity in the understory of tropical forests has rarely been examined. We aimed to identify the relative importance of the understory light environment and terrestrial bromeliads in explaining the abundance and spatial organization of different plant life forms along a coastal forest gradient from seashore inland in southeastern Brazil. We estimated the abundance of various life forms (herbs, woody plants, bromeliads, climbers, and palms) and the degree of light availability using hemispherical photographs in 165 plots (1 m2) within a 1.75 ha site. We used ordination methods, partial redundancy analysis (pRDA), spatial filtering using Moran’s eigenvector mapping, and Moran’s I splines. Forest cover was highly heterogeneous, but did not explain variation in abundance of life forms. Spatially, bromeliads were negatively associated with woody saplings, herbs and climbing plants at scales between 5-20 m, while the distance to seashore was found to be unrelated to these patterns. Our findings revealed that terrestrial bromeliads play an important role in the spatial organization of various life forms near the forest floor. Overall, the presence of terrestrial bromeliads and the plant area index better explained the understory vegetation than forest cover and distance to seashore.

https://doi.org/10.1590/0102-33062016abb0445 1277 downloads

Classification and Identification of Petroleum Microorganisms by MALDI-TOF Mass Spectrometry
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Angolini, Célio F. F.

; Pilau, Eduardo J.

; Lopes-Oliveira, Patrícia F.

; Garcia, Isabel N. S.

; Gozzo, Fábio C.

; Oliveira, Valéria M. de

; Marsaioli, Anita J.

Journal of the Brazilian Chemical Society

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mar 2015, Volume 26 N. 3 Pages 513 - 520

Abstract: En | Text: En | PDF: En

Indigenous bacteria isolated from a crude oil sample from a deep water reservoir in the Pampo Sul Oilfield (Campos Basin-RJ, Brazil) were previously classified as strains of B. pumilus. However, their enzymatic activities with fluorogenic probes and rates of petroleum biodegradation were completely different. Some of the bacteria depleted n-alkanes, whereas others did not. Aromatic compounds reported to be recalcitrant were also biodegraded by some of these Bacillus strains, revealing their outstanding ability to deplete petroleum. Further classification using matrixassisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) followed by statistical analysis revealed that these strains could be clustered into three different groups, consistent with their enzymatic activity evaluation. A more accurate phylogenetic analysis using gyrB gene sequences confirmed the MALDI-TOF MS classification of three groups of strains and identified them as Bacillus safensis, B. cereus and B. thuringiensis.

https://doi.org/10.5935/0103-5053.20150004 2146 downloads

Ação nematicida do eugenol em tomateiro
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Moreira, Lara Caroline Borges

; Vieira, Bruno Sérgio

; Mota Júnior, Célio Vinícius da

; Lopes, Everaldo Antônio

; Canedo, Ellen Júnia

Pesquisa Agropecuária Tropical

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sep 2013, Volume 43 N. 3 Pages 286 - 291

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Óleos essenciais de várias espécies de plantas possuem propriedades nematicidas e poderiam ser utilizados no controle do nematoide das galhas. Desta forma, esta pesquisa objetivou avaliar o efeito da aplicação de concentrações de eugenol no controle de Meloidogyne incognita e Meloidogyne javanica, em casa-de-vegetação. O eugenol foi aplicado nas concentrações de 0,25 mL L-1, 0,50 mL L-1, 0,75 mL L-1 e 1,00 mL L-1, na forma de pulverização foliar ou adição ao solo. O delineamento experimental foi inteiramente casualizado, em arranjo fatorial 4 x 2 + 1 (concentrações de eugenol x formas de aplicação + testemunha - tratamento adicional), com cinco repetições. Os números de galhas e de ovos de M. incognita e de ovos de M. javanica foram reduzidos em plantas tratadas com eugenol, em comparação com a testemunha. A adição ao solo foi o método mais eficiente de aplicação do eugenol, considerando-se a redução no número de galhas. Houve efeito significativo do aumento de concentrações de eugenol na redução do número de ovos de M. incognita. O eugenol apresenta potencial para ser utilizado no manejo do nematoide das galhas, embora novos estudos ainda sejam necessários para suportar tal expectativa.

Essential oils from several plant species have nematicidal properties and could be used for controlling root-knot nematodes. Thus, this study aimed at evaluating the effect of eugenol concentrations on the control of Meloidogyne incognita and Meloidogyne javanica, in a greenhouse. The eugenol was applied at the concentrations of 0.25 mL L-1, 0.5 mL L-1, 0.75 mL L-1 and 1.0 mL L-1, as a foliar spray or soil drench. A complete randomized design, in a 4 x 2 + 1 factorial scheme (concentrations of eugenol x methods of application + control - additional treatment), with five replications, was used. The number of galls and eggs of M. incognita and eggs of M. javanica decreased in plants treated with eugenol, when compared to the control. Soil drench was the most efficient method for applying eugenol, regarding the reduction in the number of galls. Increasing concentrations of eugenol reduced the number of M. incognita eggs. Eugenol showed potential to be used in the management of root-knot nematodes, though further studies are necessary to support such an expectation.

2546 downloads

Estudio Morfométrico Comparativo entre los Músculos Bíceps Braquial y Braquial con el Objetivo de Establecer el Principal Músculo Flexor del Codo
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Gusmão, Luiz Carlos Buarque de

; Sousa-Rodrigues, Célio Fernando de

; dos Santos, Eduardo José Lopes

; Pinto, Walton Nolasco Araujo

International Journal of Morphology

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mar 2012, Volume 30 N. 1 Pages 185 - 187

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

The present work seeks to compare the diameter of the wombs of the muscle brachial biceps and m. brachial in the intention of settling the doubts on which would be the most potent muscle in the flexing of the forearm. The measures were accomplished in 50 superior members of fastened corpses. A medium difference of 0.51 cm (5.8%) was evident in the diameter of the muscular womb the largest for m. brachial, ratifying what was found in the bibliographical revision, that brachial muscle is an important flexor of the elbow.

El presente trabajo se propone comparar los diámetros de los vientres del músculo bíceps braquial y músculo braquial con el objeto de esclarecer algunas dudas sobre cuál sería el músculo más potente en la flexión del antebrazo. Las comparaciones fueron realizadas en 50 miembros superiores de cadáveres fijados. Se hizo evidente la diferencia promedio de 0,51 cm (5,8%) en el diámetro del vientre muscular del músculo braquial, ratificando lo encontrado en la revisión bibliográfica, donde el músculo braquial es un importante flexor del codo.

6249 downloads

Atividade física regular preserva a função pulmonar em pacientes com espondilite anquilosante sem doença pulmonar prévia
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Goya, Karin M.

; Siqueira, Luciana T.

; Costa, Renata Alqualo

; Gallinaro, Andréa Lopes

; Gonçalves, Celio Roberto

; Carvalho, Jozélio Freire de

Revista Brasileira de Reumatologia

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apr 2009, Volume 49 N. 2 Pages 132 - 135

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A espondilite anquilosante é uma doença inflamatória que influencia de maneira restritiva a mecânica respiratória por meio do acometimento das articulações da coluna, bem como das alterações posturais decorrentes desse processo como o aumento da cifose torácica. OBJETIVO: O objetivo deste estudo foi avaliar a alteração pulmonar dos pacientes portadores de espondilite anquilosante e relacioná-la à atividade física, verificando a influência na capacidade respiratória. MÉTODOS:Foram recrutados para este estudo 104 pacientes. Apenas 15 preenchiam os critérios de inclusão e exclusão, sendo divididos em dois grupos: grupo I, composto por indivíduos sedentários, e o grupo II, composto por pacientes que praticam uma atividade física regular moderada. RESULTADOS:Os dois grupos não diferiram em relação à média da idade (49,6 ± 11,6 versus 43,3 ± 13,2 anos, p = 0,19), peso (70,9 ± 9,7 versus 74,1 ± 12,1 kg, p = 0,30) e a altura (164,6 ± 3,5 versus 167,6 ± 6,9 cm, p = 0,16). Entretanto, o tempo médio de doença foi significantemente maior no grupo I comparado ao grupo de ativos (20,1 ± 6,9 vs. 9,6 ± 3,4, p = 0,004). Como tema de interesse, observou-se que o volume minuto foi significativamente maior no grupo ativo que no inativo (4,83 ± 1,07 versus 6,1 ± 1,25, p = 0,035). Por outro lado, isso não foi demonstrado em relação à frequência respiratória (14,57 ± 1,76 versus 16,25 ± 3,53 ipm, p = 0,15), ao volume corrente (0,402 ± 0,07 versus 0,342 ± 0,10 L, p = 0,13), bem como às medidas de pressão PI máx (84,29 ± 24,99 versus 93,13 ± 16,76 cmH2O), p = 0,24) e PE máx (102,2 ± 29,26 versus 105 ± 17,32 cmH2O, p = 0,42). CONCLUSÃO:Este estudo pareceu demonstrar que os volumes pulmonares são mantidos em pacientes com espondilite anquilosante que realizam atividade física regular.

Ankylosing spondylitis is an inflammatory condition which causes restriction in the lung function due to column joint alterations leading to postural dysfunction and thoracic ciphosis. OBJECTIVE: The objective of this study was to evaluate the lung function in patients with ankylosing spondylitis and to associate this parameter with physical activity. METHODS:104 patients were selected and only 15 fulfilled inclusion and exclusion criteria. They were divided in two groups: group I composed by sedentary patients, and group II with patients that has a regular physical activity. Demographic features, parameters of cervical, dorsal and lumbar column mobility were collected. Measurements of minute volume, respiratory rate, maximum inspiratory and expiratory pressures and quality of life (HAQ-S) were also evaluated. RESULTS: Both groups were similar regarding mean age (49.6 ± 1.6 vs. 43.3 ± 13.2 years, p = 0.19), weight (70.9 ± 9.7 vs. 74.1 ± 12.1 kg, p = 0.30) and height (164.6 ± 3.5 vs. 167.6 ± 6.9 cm, p = 0.16). However, the mean disease duration was significantly higher in the group I compared to the active group (20.1 ± 6.9 vs. 9.6 ± 3.4 years, p = 0.004). Interestingly, a significantly higher minute volume was observed in the active group than inactive one (4.83 ± 1.07 vs. 6.1 ± 1.25 L/min, p = 0.035). On the other hand, no differences were found regarding respiratory rate (14.57 ± 1.76 vs. 16.25 ± 3.53 ipm, p = 0.15), tidal volume (0.402 ± 0.07 vs. 0.342 ± 0.10 L, p = 0.13), as well as maximum inspiratory pressure (84.29 ± 24.99 vs. 93.13 ± 16.76 cmH2O), p = 0.24) and maximum expiratory pressure (102.2 ± 29.26 vs. 105 ± 17.32 cmH2O, p = 0.42). ), CONCLUSION: This study seems to show that respiratory volumes are maintained stable in patients with ankylosing spondylitis that are under regular physical activity.

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Immunological and microbiological activity of Davilla elliptica St. Hill. (Dilleniaceae) against Mycobacterium tuberculosis
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Lopes, Flávia Cristine Mascia

; Placeres, Marisa Campos Polesi

; Jordão Junior, Cleso Mendonça

; Higuchi, Célio Takashi

; Rinaldo, Daniel

; Vilegas, Wagner

; Leite, Clarice Queico Fujimura

; Carlos, Iracilda Zeppone

Memórias do Instituto Oswaldo Cruz

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sep 2007, Volume 102 N. 6 Pages 769 - 772

Abstract: En | Text: En | PDF: En

Mycobacterium tuberculosis is responsible for over 8 million cases of tuberculosis (TB) annually. Natural products may play important roles in the chemotherapy of TB. The immunological activity of Davilla elliptica chloroform extract (DECE) was evaluated in vitro by the determination of hydrogen peroxide (H2O2), nitric oxide (NO), and tumor necrosis factor-alpha (TNF-alpha) release in peritoneal macrophages cultures. DECE was also tested for its antimycobacterial activity against M. tuberculosis using the microplate alamar blue assay. DECE (50, 150, 250 µg/ml) stimulated the production of H2O2 (from 1,79 ± 0,23 to 7,27 ± 2,54; 15,02 ± 2,86; 20,5 ± 2,1 nmols) (means ± SD), NO (from 2,64 ± 1,02 to 25,59 ± 2,29; 26,68 ± 2,41; 29,45 ± 5,87 µmols) (means ± SD) and TNF-alpha (from 2,44 ± 1,46 to 30,37 ± 8,13; 38,68 ± 1,59; 41,6 ± 0,90 units/ml) (means ± SD) in a dose-dependent manner and also showed a promising antimycobacterial activity with a minimum inhibitory concentration of 62,5 µg/ml. This plant may have therapeutic potential in the immunological and microbiological control of TB.

1691 downloads

II Consenso Brasileiro de Tuberculose: Diretrizes Brasileiras para Tuberculose 2004
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Castelo Filho, Adauto

; Kritski, Afrânio Lineu

; Barreto, Ângela Werneck

; Lemos, Antonio Carlos Moreira

; Netto, Antonio Ruffino

; Guimarães, Carlos Alberto

; Silva, Célio Lopes

; Sant'anna, Clemax do Couto

; Haddad, David Jamil

; Lima, Dinalva Soares

; Matos, Eliana Dias

; Melo, Fernanda Carvalho de Queiroz

; Melo, Fernando Augusto Fiúza de

; Gerhardt Filho, Germano

; Marsico, Giovanni Antonio

; Silva, Guida

; Siqueira, Hélio Ribeiro

; Campos, Hisbello

; Saconato, Humberto

; Dourado, Inês

; Rosemberg, José

; Braga, José Ueleres

; Santos, Joseney Raimundo dos

; Seiscento, Márcia

; Conde, Marcus Barreto

; Dalcolmo, Margareth Pretti

; Almeida, Margarida Mattos Brito de

; Penna, Maria Lucia Fernandes

; Barreto, Maurício L.

; Hijjar, Miguel Aiub

; Andrade, Mônica Kramer de Noronha

; Cardoso, Ninarosa Calvazara

; Pineda, Norma Irene Soza

; Leite, Olavo Henrique Munhoz

; Picon, Pedro

; Silva, Rodney Frare e

; Cavalcanti, Solange

; Pereira, Susan M.

; Augusto, Valéria Maria

; Galesi, Vera

; Pinto, Walkyria Pereira

Jornal Brasileiro de Pneumologia

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jun 2004, Volume 30 Suppl. 1 Pages S57 - S86

Abstract: Pt | Text: Pt | PDF: Pt

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