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[SciELO Preprints] - Guidelines on the Diagnosis and Treatment of Hypertrophic Cardiomyopathy – 2024
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Fernandes, Fabio Simões, Marcus V. Correia, Edileide de Barros Marcondes-Braga, Fabiana G. Coelho-Filho, Otavio Rizzi Mesquita, Cláudio Tinoco Mathias-Junior, Wilson Rochitte, Carlos Eduardo Ramires, Felix José Alvarez Alves, Silvia Marinho Martins Montera, Marcelo Westerlund Lopes, Renato Delascio Oliveira-Junior, Mucio Tavares Scolari, Fernando L. Avila, Walkiria Samuel Canesin, Manoel Fernandes Bacal, Fernando Bocchi, Edimar Alcides Moura, Lídia Ana Zytynski Saad, Eduardo Benchimol Scanavacca, Mauricio I. Valdigem, Bruno Pereira Cano , Manuel Nicolas Abizaid , Alexandre Ribeiro, Henrique Barbosa Lemos-Neto, Pedro Alves Ribeiro, Gustavo Calado de Aguiar Jatene, Fabio Biscegli Dias, Ricardo Ribeiro Beck-da-Silva, Luis Rohde, Luis Eduardo P. Bittencourt, Marcelo Imbroinise Pereira, Alexandre Krieger, José Eduardo Villacorta, Humberto Martins, Wolney de Andrade Figueiredo-Neto, José Albuquerque de Cardoso , Juliano Novaes Pastore, Carlos Alberto Jatene, Ieda Biscegli Tanaka, Ana Cristina Sayuri Hotta, Viviane Tiemi Romano, Minna Moreira Dias Albuquerque, Denilson Campos de Mourilhe-Rocha, Ricardo Hajjar, Ludhmila Abrahão Brito, Fabio Sandoli de Caramelli , Bruno Calderaro, Daniela Farsky, Pedro Silvio Colafranceschi , Alexandre Siciliano Pinto, Ibraim Masciarelli Vieira , Marcelo Luiz Campos Danzmann, Luiz Claudio Barberato , Silvio Henrique Mady, Charles Martinelli-Filho, Martino Torbey , Ana Flavia Malheiros Schwartzmann, Pedro Vellosa Macedo, Ariane Vieira Scarlatelli Ferreira , Silvia Moreira Ayub Schmidt, Andre Melo , Marcelo Dantas Tavares de Lima-Filho, Moysés Oliveira Sposito, Andrei C. Brito, Flavio de Souza Biolo, Andreia Madrini-Junior, Vagner Rizk, Stéphanie Itala Mesquita, Evandro Tinoco

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Publication date: 2024-04-08

Abstract: Pt Es En

A cardiomiopatia hipertrófica (CMH) é uma forma de doença do músculo cardíaco de causa genética, caracterizada pela hipertrofia das paredes ventriculares. O diagnóstico requer detecção por métodos de imagem (Ecocardiograma ou Ressonância Magnética Cardíaca) de qualquer segmento da parede do ventrículo esquerdo com espessura > 15 mm, sem outra causa provável. A análise genética permite identificar mutações de genes codificantes de diferentes estruturas do sarcômero responsáveis pelo desenvolvimento da CMH em cerca de 60% dos casos, permitindo o rastreio de familiares e aconselhamento genético, como parte importante do manejo dos pacientes e familiares. Vários conceitos sobre a CMH foram recentemente revistos, incluindo sua prevalência de 1 em 250 indivíduos, não sendo, portanto, uma doença rara, mas subdiagnosticada. A vasta maioria dos pacientes é assintomática. Naqueles sintomáticos, a obstrução do trato de saída do ventrículo esquerdo (OTSVE) é o principal distúrbio responsável pelos sintomas, devendo-se investigar a sua presença em todos os casos. Naqueles em que o ecocardiograma em repouso ou com Manobra de Valsalva não detecta gradiente intraventricular significativo (> 30 mmHg), devem ser submetidos à ecocardiografia com esforço físico para detecção da OTSVE.   Pacientes com sintomas limitantes e grave OTSVE, refratários ao uso de betabloqueadores e verapamil, devem receber terapias de redução septal ou uso de novas drogas inibidoras da miosina cardíaca. Por fim, os pacientes adequadamente identificados com risco aumentado de morta súbita podem receber medida profilática com implante de cardiodesfibrilador implantável (CDI).

La miocardiopatía hipertrófica (MCH) es una forma de enfermedad cardíaca de origen genético, caracterizada por el engrosamiento de las paredes ventriculares. El diagnóstico requiere la detección mediante métodos de imagen (Ecocardiograma o Resonancia Magnética Cardíaca) que muestren algún segmento de la pared ventricular izquierda con un grosor > 15 mm, sin otra causa probable. El análisis genético permite identificar mutaciones en genes que codifican diferentes estructuras del sarcómero responsables del desarrollo de la MCH en aproximadamente el 60% de los casos, lo que permite el tamizaje de familiares y el asesoramiento genético, como parte importante del manejo de pacientes y familiares. Varios conceptos sobre la MCH han sido revisados recientemente, incluida su prevalencia de 1 entre 250 individuos, por lo tanto, no es una enfermedad rara, sino subdiagnosticada. La gran mayoría de los pacientes son asintomáticos. En los casos sintomáticos, la obstrucción del tracto de salida ventricular izquierdo (TSVI) es el trastorno principal responsable de los síntomas, y su presencia debe investigarse en todos los casos. En aquellos en los que el ecocardiograma en reposo o la maniobra de Valsalva no detecta un gradiente intraventricular significativo (> 30 mmHg), deben someterse a ecocardiografía de esfuerzo para detectar la obstrucción del TSVI. Los pacientes con síntomas limitantes y obstrucción grave del TSVI, refractarios al uso de betabloqueantes y verapamilo, deben recibir terapias de reducción septal o usar nuevos medicamentos inhibidores de la miosina cardíaca. Finalmente, los pacientes adecuadamente identificados con un riesgo aumentado de muerte súbita pueden recibir medidas profilácticas con el implante de un cardioversor-desfibrilador implantable (CDI).

Hypertrophic cardiomyopathy (HCM) is a form of genetically caused heart muscle disease, characterized by the thickening of the ventricular walls. Diagnosis requires detection through imaging methods (Echocardiogram or Cardiac Magnetic Resonance) showing any segment of the left ventricular wall with a thickness > 15 mm, without any other probable cause. Genetic analysis allows the identification of mutations in genes encoding different structures of the sarcomere responsible for the development of HCM in about 60% of cases, enabling screening of family members and genetic counseling, as an important part of patient and family management. Several concepts about HCM have recently been reviewed, including its prevalence of 1 in 250 individuals, hence not a rare but rather underdiagnosed disease. The vast majority of patients are asymptomatic. In symptomatic cases, obstruction of the left ventricular outflow tract (LVOT) is the primary disorder responsible for symptoms, and its presence should be investigated in all cases. In those where resting echocardiogram or Valsalva maneuver does not detect significant intraventricular gradient (> 30 mmHg), they should undergo stress echocardiography to detect LVOT obstruction. Patients with limiting symptoms and severe LVOT obstruction, refractory to beta-blockers and verapamil, should receive septal reduction therapies or use new drugs inhibiting cardiac myosin. Finally, appropriately identified patients at increased risk of sudden death may receive prophylactic measure with implantable cardioverter-defibrillator (ICD) implantation.

https://doi.org/10.1590/SciELOPreprints.8394

Floral ecology of Puya ctenorhyncha (Bromeliaceae) an endemic plant of Bolivia
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Velásquez-Noriega, Paola

; Krömer, Thorsten

; Pacheco, Luis F.

Botanical Sciences

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mar 2024, Volume 102 N. 1 Pages 68 - 82

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Background: The terrestrial bromeliad Puya ctenorhyncha is a near-threatened endemic species that grows between 2,500-4,050 m asl. Hypotheses: Hummingbirds are the most important visitors of this plant given its morphological and floral traits. Study site: The study was carried out along the “Death Road”, located in the Yungas montane cloud forest, La Paz, Bolivia. Methods: We monitored an average of 216 individuals monthly for two years (2018-2019). Besides we conducted direct observations of focal plants to quantify the visits of animals. Results: The reproductive season lasted from April to August in the first year and from May to June in the second. The mean nectar volume was 8.09 ± 1.59 μL per flower and its composition included fructose, glucose, and 86 % of water. With 341 hours of observations, we determined that Coeligena torquata (Trochilidae) is the main visitor and putative pollinator, followed by C. violifer. Males of both species were more frequent visitors than females. Coeligena torquata showed the highest activity during the afternoon while C. violifer was more active in the morning. Conclusions: Floral phenology seems to be annual and unimodal. Although the main pollinators seem to be hummingbirds, the nectar concentration and composition is more similar to bromeliad species of other genera which are pollinated by bats or passerine birds. It is important to evaluate, in future studies, the dependence of both hummingbirds and their behavioural response towards the plant.

Resumen: Antecedentes: La bromelia terrestre Puya ctenorhyncha es una especie endémica casi amenazada que crece entre los 2,500-4,050 m snm. Hipótesis: Los colibríes son los visitantes más importantes de esta planta dadas sus características morfológicas y florales. Sitio de estudio: El estudio se realizó en el “Camino de la Muerte”, situado en el bosque nublado montano de los Yungas, La Paz, Bolivia. Métodos: Monitoreamos un promedio de 216 individuos mensuales durante dos años (2018-2019), mediante observaciones de su biología fenológica. Asimismo, realizamos observaciones directas en plantas focales para cuantificar las visitas de animales. Resultados: La época reproductiva duró de abril a agosto en el primer año y de mayo a junio en el segundo. El volumen medio de néctar fue de 8,09 ± 1,59 μL por flor y su composición incluía fructosa, glucosa y 86 % de agua. Con 341 horas de observaciones, determinamos que Coeligena torquata (Trochilidae) es el principal visitante y polinizador putativo, seguido por C. violifer. Los machos de ambas especies fueron visitantes más frecuentes que las hembras. Coeligena torquata mostró la mayor actividad durante la tarde mientras que C. violifer fue más activa por la mañana. Conclusiones: La fenología floral parece ser anual y unimodal. Aunque los principales polinizadores parecen ser los colibríes, la concentración y composición del néctar es más similar a especies de bromelias de otros géneros que son polinizadas por murciélagos o aves paseriformes. Es importante evaluar, en estudios futuros, la dependencia de ambos colibríes y su respuesta de comportamiento a la planta.

https://doi.org/10.17129/botsci.3393

Multiple Liver Nodules in Fontan-Associated Liver Disease
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Mendes, Raquel R.

; Marques, Luis

; Figueiredo, Pedro C.

GE-Portuguese Journal of Gastroenterology

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feb 2024, Volume 31 N. 1 Pages 70 - 72

Abstract: En | Text: En | PDF: En

https://doi.org/10.1159/000528462

Supramolecular Assembly between Cationic Pyridinium-Pillararene and Aminosalicylate Drug
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Demos, Willian

; Micke, Lizandra C. B.

; Lacerda, Luis H. S.

; Souza, Bruno S.

; Gerola, Adriana P.

; Affeldt, Ricardo F.

Journal of the Brazilian Chemical Society

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2024, Volume 35 N. 1 elocation e-20230106

Abstract: En | Text: En | PDF: En

The preparation and characterization of a novel pillar[5]arene based supramolecular system with mesalazine, a prescribed aminosalicylate drug was successfully achieved. Pyridinium-pillar[5]arene was efficiently prepared in three steps from hydroquinone bis(2-hydroxyethyl) ether derivative in high yield. It was demonstrated that the association between the compounds is pH dependent with an association constant of 3.4 × 103 L mol-1 near physiological conditions, leading to quenching of the fluorescence emission of mesalazine in the presence of the cationic macrocycle. Theoretical calculations indicate that the association between both molecules is of electrostatic nature. Formation of aggregates was also demonstrated in low concentrations of pillar[5]arene that is related to the non-linear behavior of the Stern-Volmer plot for quenching of mesalazine fluorescence emission.

https://doi.org/10.21577/0103-5053.20230106

Lista anotada de cactus nativos y naturalizados de Cuba
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Barrios, Duniel

; Arias, Salvador

; González-Torres, Luis Roberto

; Majure, Lucas C.

Botanical Sciences

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dec 2023, Volume 101 N. 4 Pages 1249 - 1300

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Antecedentes: El archipiélago cubano posee la mayor diversidad de cactus en el Caribe. Pese a que en los últimos años se han realizado numerosos estudios que facilitan la delimitación de sus especies, los inventarios existentes aún no han considerado toda la evidencia disponible. Preguntas: ¿Cuántos cactus nativos y naturalizados hay en Cuba? ¿Qué porcentaje de los cactus nativos son endémicos? Especies de estudio: Familia Cactaceae. Sitio y años de estudio: Cuba, 2013-2023. Métodos: Se revisó la información disponible sobre cactáceas cubanas donde se incluyeran listas o descripciones de especies, estudios taxonómicos, sistemáticos y filogenéticos. Además, se realizó un extenso trabajo de campo durante una década que abarcó toda la isla de Cuba. Se consideraron especies nativas las que están presentes en el archipiélago debido a causas naturales y que no llegaron debido a la intervención humana, mientras como especie naturalizada se consideraron las que fueron introducidas, intencional o no, por diferentes actividades humanas y que pueden reproducirse (sexual o asexual) de forma regular y mantener poblaciones por varias generaciones sin la intervención antrópica. Resultados: Registramos para Cuba 71 especies nativas o naturalizadas de 20 géneros de cactus, de estas, 49 especies son nativas (75.51 % endémicas) y 22 naturalizadas. Conclusiones: Cuba se ratifica como la isla con mayor diversidad de cactus en el Caribe.

Abstract Background: The Cuban archipelago has the greatest diversity of cactus in the Caribbean. Although in recent years there have been numerous studies that facilitate the delimitation of their species, existing inventories have not yet considered all available evidence. Questions: How many native and naturalized cacti are there in Cuba? What percentage of native cacti are endemic? Studied species: Cactaceae family. Study site and dates: Cuba, 2013-2023. Methods: The information available on Cuban cacti was reviewed that includes lists or descriptions of species, taxonomic, systematic and phylogenetic studies. In addition, an extensive field work was carried out for a decade that covered the entire island of Cuba. Native species were considered that are present in the archipelago due to natural causes and that did not arrive due to human intervention, while as a naturalized species, those that were introduced, intentional or not, by different human activities and that can be reproduced (sexual or asexual) regularly and maintain populations for several generations without anthropic intervention. Results: We recorded for Cuba 71 native or naturalized species of 20 genera of cacti, of these, 49 species are native (75.51 % endemic) and 22 naturalized. Conclusions: Cuba is ratified as the island with greater diversity of cactus in the Caribbean.

https://doi.org/10.17129/botsci.3324

Nanopartículas metálicas: una alternativa para combatir la resistencia de especies causantes de la candidiasis
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García-Marín, Luis Enrique

; Castro-Longoria, Ernestina

Mundo nano. Revista interdisciplinaria en nanociencias y nanotecnología

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dec 2023, Volume 16 N. 31 elocation 69780

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN La candidiasis es provocada por diferentes especies del género Candida, siendo C. albicans la más común en los aislados clínicos a nivel mundial. Esta enfermedad es un problema para el sector salud, debido a la multirresistencia que algunas especies de Candida presentan a los antifúngicos tradicionalmente utilizados para controlar la enfermedad. Tal es el caso de C. glabrata y recientemente C. auris, las cuales son resistentes a los azoles, las equinocandinas y a los polienos; por lo cual, la búsqueda de nuevos antifúngicos es una prioridad. La nanotecnología ofrece nuevas alternativas, como el uso de las nanopartículas (NPs) metálicas, en particular, las de plata y cobre han mostrado ser eficientes para la inhibición de C. albicans. En este trabajo se presentan los estudios más relevantes que han evaluado el efecto de las NPs metálicas en especies del género Candida, así como los resultados más prometedores.

ABSTRACT Candidiasis is a disease caused by different Candida species, with C. albicans being the most common in clinical isolates worldwide. This disease is a problem for the health sector, due to the multiresistance that some Candida species present to the antifungals traditionally used. C. glabrata and C. auris are two species frequently reported as resistant to azoles, echinocandins and polyenes. Therefore, the search for new antifungals is a priority. Nanotechnology offers new alternatives, such as the use of metallic nanoparticles (NPs). In particular, silver and copper NPs have been reported as efficient inhibition agents against C. albicans. This paper presents the most relevant studies that have evaluated the effect of metallic NPs in Candida spp., as well as the most promising results.

https://doi.org/10.22201/ceiich.24485691e.2023.31.69780

Resistencia antimicrobiana en los principales recursos acuícolas de los departamentos de Tumbes, Piura, San Martin y Puno
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Calizaya, Carla D.

; Reátegui, Erick H.

; Silva, José C.

; Velazco, Rodolfo

; Rodríguez, Liliana

; Angulo, Derly R.

; Llontop, Carlos A.

; Salcedo, Luis A.

; Villena, Carla A.

Uniciencia

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dec 2023, Volume 37 N. 1 Pages 562 - 577

Abstract: En Es Pt | Text: En Es Pt | PDF: En | PDF: Es | PDF: Pt

Abstract (Objective) The objective of this study was to analyze antimicrobial resistance in Peruvian aquaculture. (Methodology) An observational, descriptive study was carried out. Samples of Argopectenpurpuratus, Oncorhynchus mykiss, Oreochromis niloticus and Litopenaeus vannamei were included. The bacteria Enterococcus spp., Salmonella spp., Erichia coli, Vibrio parahaemolyticus, Aeromonas spp., and Flavobacterium psychrophilum were isolated. Antibiotic resistance was evaluated using antibiograms, and resistant samples were sequenced to identify antimicrobial resistance genes. Absolute and percentage frequencies were calculated. In addition, Wald, Clopper-Pearson, and pooled prevalence confidence intervals were estimated. (Results) In the case of A. purpuratus, 22.2% (95% CI:2.81-60.00) of the E. coli samples were resistant to oxytetracycline. Resistant samples were found in 4.44% (n=2) of the aquaculture production centers (APCs). In O. mykiss, 88.89% (95% CI:51.16-99.72) of the Enterococcus spp. were resistant to osfomycin and oxytetracycline. Resistant samples were found in12.96% of the APCs (n=7). Regarding O. niloticus, 47.31% (95% CI: 37.16-57.46) of the E. coli samples were resistant to oxytetracycline ampicilline>chloramphenicol>fosfomycin and azithromycin. Resistant samples were encountered in 50% of the APCs (n=19). In addition, 89.36% (95% CI:80.55-98.18) of the Enterococcus spp. were resistant to Fosfomycin> chloranphenicol and colistin> oxytetracycline>azithromycin>ampicilline; resistant samples were found in 36.84% of the APCs (n=14). In L. vannamei, 87.11% (95% CI:83.00-91.21) of the Enterococcus spp. samples were resistant to Fosfomycin> colistin> oxytetracycline> azithromycin> ampicilline and resistant samples were found in 100% of the APCs (n=33). Likewise, 35.05% (95% CI:29.74-40.23) of the V. parahaemolyticus samples were resistant to ampicilline>fosfomycin>oxytetracycline>azithromycin>eritromicine>enrofloxacin>ampicilline> chloranfhenicol. Resistant samples were found in 78.79% (n=26) of the APCs (Conclusions) The percentage of APCs with O. niloticus and L. vannamei showed that antimicrobial resistance was high.

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Resumo (Objetivo) O objetivo foi analisar a resistência antimicrobiana na aquicultura peruana. (Metodologia) Foi realizado um estudo observacional, descritivo. Foram incluídas amostras das espécies Argopecten purpuratus, Oncorhynchus mykiss, Oreochromis niloticus e Litopenaeus vannamei. Foram isoladas bactérias dos gêneros Enterococcus spp., Salmonella spp., Aeromonas spp., e espécies de Escherichia coli, Vibrio parahaemolyticus e Flavobacterium psychrophilum. A resistência antimicrobiana foi avaliada por meio de antibiograma, e amostras resistentes foram sequenciadas para identificar genes de resistência antimicrobiana. Foram calculadas as frequências absoluta e percentual, além dos intervalos de confiança de Wald, Clopper-Pearson e as prevalências agrupadas. (Resultados) Em relação a A. purpuratus, 22,2% (IC 95%: 2,81-60,00) das amostras de E. coli foram resistentes à oxitetraciclina. Estes pertenciam a 4,44 % (n=2) dos centros de produção aquícola (CPA). Em O. mykiss, 88,89% (IC 95%: 51,16-99,72) das amostras de rococcus spp. foram resistentes à fosfomicina e oxitetraciclina. Estas pertenciam a 12,96% das CPAs (n=7). Em relação a O. niloticus, 47,31 % IC 95%: 37,16-57,46) das amostras de E. coli foram resistentes à oxitetraciclina>ampicilina>cloranfenicol fosfomicina e azitromicina. Estas pertenciam a 50% das CPAs (n=19). Além disso, 89,36% (IC95%: 80,55-98,18) das amostras de Enterococcus spp. foram resistentes a fosfomicina>cloranfenicol e colistina>oxitetraciclina>azitromicina>ampicilina; estas pertenciam a 36,84% das CPAs (n=14). Em L. vannamei, 87,11% (IC 95%: 83,00-91,21) das amostras de Enterococcus spp foram resistentes à fosfomicina>colistina>oxitetraciclina>azitromicina eritromicina>enrofloxacina> ampicilina>cloranfenicol e pertenciam a 100% das CPAs (n=33). Além disso, 3 ,05% (IC 95%: 29,74-40,23) das amostras de V.parahaemolyticus foram resistentes à ampicilina>fosfomicina>colistina>oxitetraciclina>azitromicina. Estas pertenciam a 78,79% (n=26) das CPAs. (Conclusões) A porcentagem de CPA de O. niloticus L. vannamei, mostrou maior resistência antimicrobiana do estudo.

https://doi.org/10.15359/ru.37-1.30

Corrected sinus node recovery time as a screening test in patients with typical atrial flutter post-ablation to predict pacemaker implantation
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Reinoso-Gonell, Reynie L.

; Céspedes-Batista, Yasmín

; Batlle-Núñez, Nicolle M.

; Madera-Ferreira, Irvianny

; Gutiérrez-Martínez, Anthony

; Morales-Velázquez, José L.

; Nava-Townsend, Santiago

; Márquez-Murillo, Manlio

; Tejera-Jurado, Luis C.

; Gómez-Flores, Jorge R.

; Colín-Lizalde, Luis

; Iturralde-Torres, Pedro

Archivos de cardiología de México

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dec 2023, Volume 93 N. 4 Pages 398 - 404

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Objetivo: Establecer el valor pronóstico del TRNSC basado en la necesidad de marcapasos en pacientes diagnosticados con aleteo atrial, pos-ablación. Métodos: Este cohorte prospectivo, realizado en el Instituto Nacional de Cardiología "Ignacio Chávez" en la Ciudad de México, evaluó pacientes sometidos a ablación para corregir el aleteo atrial; se midió el TRNSC post bloqueo autonómico. Resultados: La muestra de 40 pacientes se subdividió en 2 grupos según su requerimiento de marcapasos posterior a la ablación (P y NP). Se diagnosticó disfunción del nodo sinusal en 13 participantes (32.5%), de los cuales 10 (71.43%) requirieron marcapasos en comparación a 4 (28.57%) con función normal. En el grupo P la pausa del nodo sinusal post-ablación tuvo una media de 1.86 ± 0.96 s versus el grupo NP con 1.196 ± 0.52 s. En relación con el TRNSC, el grupo NP tuvo una media de 383.54 ± 67.96 ms vs. 1972.57 ± 3423.56 ms en el grupo P. 10 pacientes (25%) obtuvieron un TRNSC > 500 ms, de los cuales 100% requirieron marcapasos; de los 14 pacientes que requirieron marcapasos 10 (71.43%) tenían un TRNSC elevado (p ≤ 0.01). Conclusiones: El TRNSC tiene el potencial de ser una herramienta de pronóstico cuantitativo para la necesidad de futuros implantes de marcapasos en pacientes con disfunción del nodo sinusal, resultado de aleteo atrial pos-ablación. Esto podría ayudar a diagnosticar más temprano una disfunción del nodo sinusal, resultando en la reducción de la pérdida a largo plazo de la función cardíaca como efecto de la remodelación.

Abstract Objective: The objective of the study was to establish the prognostic value of CSNRT regarding the necessity for pacemaker implantation in patients with atrial flutter (AFL) post-ablation. Methods: This prospective cohort study, conducted at the National Institute of Cardiology "Ignacio Chavez" in Mexico City, assessed patients who had undergone ablation procedures to correct AFL, posterior to which an autonomic blockade was performed, and CSNRT was measured. Results: The sample for this investigation was 40 patients. These were subdivided into two study groups depending on their requirement of pacemaker implant post-ablation (Pacemaker P, No Pacemaker NP). Sinus node (SN) dysfunction was diagnosed in 13 (32.5%) of the 40 participants, 10 (71.43%) of which required a pacemaker implant, while only 4 participants (28.57%) with normal SN function required pacemakers. Ten out of the 14 patients (71.43%) who required a pacemaker had an elevated CSNRT > 500 ms (p ≤ 0.01). Post-ablation CSNRT mean was 383.54 ms ± 67.96 ms in the NP group versus 1972.57 ms ± 3423.56 ms in the P group. Furthermore, SN pause in the P group had a mean of 1.86 s ± 0.96 s versus the NP group with 1.196 s ± 0.52 s. Conclusion: CSNRT has the potential to be a quantitative prognostic tool for the assessment of future pacemaker implants in patients with AFL post-ablation. This could aid in the timely diagnosis of sinus node dysfunction, which could, in the long run, result in the reduction of cardiac functional capacity loss due to cardiac remodeling.

https://doi.org/10.24875/acm.22000031

Challenges in prosthetic valvular thrombosis: imaging relevance and the decision-making process
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Herrera-Céspedes, Cristhian E.

; Giraldo, Santiago

; Saavedra, Yesid

; Vélez, Luis A.

; Hernández, José A.

; López, Nilson

; Chavarriaga, Juan C.

Archivos de cardiología de México

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dec 2023, Volume 93 N. 4 Pages 502 - 503

Abstract: En | Text: En | PDF: En

https://doi.org/10.24875/acm.22000193

10.

Hypercalcemic crisis as a presentation of primary hyperparathyroidism
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Bedini, Iván

; Sylvestre Begnis, Gustavo

; Poenitz, Fernando

; Polillo, David C.

; Vargas, María Cecilia

; Bolzán, Diamela

; Ramírez Stieben, Luis A.

Medicina (Buenos Aires)

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dec 2023, Volume 83 N. 5 Pages 804 - 807

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Hyperparathyroidism-induced hypercalcemic crisis (HIHC) is an unusual state of marked progressive pri mary hyperparathyroidism (PHPT). Patients have severe hypercalcemia and may have severe symptoms such as kidney failure, acute pancreatitis, and mental changes. PHPT is due to the presence of a single gland adenoma/ disease in 80 to 85%; parathyroid carcinoma is reported in <1%. Among patients with adenoma, atypical parathy roid tumor can be found infrequently. Parathyroidectomy is the only curative approach for PHPT. In this report we present three cases of HIHC due to giant parathyroid adenomas (GPAs), one of them with histopathological characteristics of an atypical parathyroid tumor, with satisfactory evolution after parathyroidectomy.

Resumen La crisis hipercalcémica inducida por hiperparatiroi dismo (HIHC) es un estado inusual de hiperparatiroidis mo primario progresivo y marcado (HPTP). Los pacientes tienen hipercalcemia grave y pueden tener síntomas graves como insuficiencia renal, pancreatitis aguda y cambios mentales. El HPTP se debe a la presencia de un adenoma/enfermedad de una sola glándula en 80 a 85%; el carcinoma de paratiroides se informa en <1%. Entre los pacientes con adenoma, el tumor paratiroideo atípico se puede encontrar con baja frecuencia. La paratiroidec tomía es el único abordaje curativo del HPTP. En este reporte presentamos tres casos de HIHC por adenomas paratiroideos gigantes (APGs), uno de ellos con características histopatológicas de tumor paratiroideo atípico, con evolución satisfactoria luego de paratiroidectomía.

11.

Effect of school reopening on pediatric morbidity and mortality during the third epidemiological wave of COVID-19 in a Mexican state
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Reyna-Figueroa, Jesús

; Mirón-Calderón, Xochitl

; Durán-Mendieta, Victor

; Ramirez-Gijón, Guillermo

; Torres-Meza, Victor

; Anaya-López, Luis

; Frías-Badillo, Juan C.

; Mejía-Martínez, Valeria

; Salyano-Peñuelas, Yolanda A.

; Diaz-Martínez, Alfredo I.

; Fernández-Clamont, Francisco J.

Boletín médico del Hospital Infantil de México

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dec 2023, Volume 80 N. 6 Pages 367 - 373

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Introducción: La determinación del efecto de reabrir las escuelas sobre las tasas de infección pediátrica por SARS-CoV-2 (síndrome respiratorio agudo grave coronavirus 2) incrementó la necesidad de trasmitir la experiencia de los gobiernos de muchas regiones geográficas para mejores decisiones futuras en emergencias sanitarias similares. Métodos: Mediante un estudio prospectivo basado en una cohorte poblacional se dio seguimiento a los alumnos de 18,988 escuelas del Estado de México que iniciaron con el regreso a clases. Se implementaron filtros sanitarios diarios en cada escuela y cotidianamente se informaban a los enlaces jurisdiccionales a través de una red negativa. Los casos identificados eran confirmados a través de RT-PCR (reacción en cadena de la polimerasa con transcriptasa inversa). Se estimaron frecuencias simples de casos, porcentajes e incidencias de COVID-19. Las incidencias del estado se compararon con la incidencia nacional. Resultados: Un total de 3,586 casos fueron confirmados; 2,048 (57.1%) correspondieron a niños. Veinticuatro (0.6%) fueron hospitalizados por COVID-19 moderado a grave; nueve (37.5%) fallecieron, y solamente una correspondió a un escolar. A partir de la semana 36 se observó una tasa promedio de infecciones de 0.36. En la semana epidemiológica 40 se observó la mayor tasa de infección en escolares (1.01); a partir de esta semana se observa un declive de los casos hasta la semana 50. Conclusiones: La implementación de intervenciones no farmacéuticas tiene más ventajas que limitaciones, siempre y cuando las estrategias sean homogéneas y correctamente ejecutadas, lo que asegurará un adecuado control en los contagios.

Abstract Background: Determining the effect of reopening schools on pediatric SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) infection rates increased the need to share the experience of governments in many geographic regions for better future decision-making in similar health emergencies. Methods: Through a prospective study based on a population-based cohort, students from 18,988 schools in the State of Mexico who began returning to school were followed. Daily sanitation filters were implemented in each school and district liaisons were informed on a daily basis through a negative network. Identified cases were confirmed by reverse transcriptase-polymerase chain reaction. Simple case frequencies, percentages, and incidences of COVID-19 were estimated. State incidences were compared with the national incidence. Results: A total of 3,586 cases were confirmed; 2,048 (57.1%) were children. Twenty-four (0.6%) were hospitalized for moderate to severe COVID-19; nine (37.5%) died, and only one was a schoolchild. From week 36, an average infection rate of 0.36 was observed. The highest infection rate in schoolchildren was observed in epidemiologic week 40 (1.01); from this week on, a decrease in the number of cases was observed until week 50. Conclusions: The use of non-pharmaceutical interventions has more advantages than limitations, as long as the strategies are homogeneous and properly implemented to ensure adequate control of infections.

https://doi.org/10.24875/bmhim.23000075

12.

Perfil de la biomasa aérea acumulada en verano de Crotalaria pumila Ort.
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Martínez-Zaragoza, Jesús Adán

; Martínez-Hernández, Pedro Arturo

; Zaragoza-Ramírez, José Luis

; Cortés-Díaz, Enrique

Revista mexicana de ciencias agrícolas

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nov 2023, Volume 14 N. 7 elocation e3199

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Crotalaria pumila Ort es una leguminosa nativa de México con poca información sobre su potencial forrajero con base en su perfil de acumulación durante el verano, por lo que se realizó un estudio con el objetivo de determinar el rendimiento total y por componente durante 2021, para generar información base para decidir sobre la posibilidad de la especie como forrajera. En un área con C. pumila espontánea, se realizaron nueve muestreos semanales, en los que se determinó rendimiento total y por componente: hoja, tallo, flor y vainas, su aporte proporcional al total y número por planta y altura de planta. El análisis estadístico fue para un diseño completamente al azar con tres repeticiones, la variable independiente fue el número de días transcurridos en el verano. Conforme avanzó el verano, los rendimientos totales y de vaina se incrementaron (p< 0.05) el de tallo se mantuvo constante (p> 0.05) y el de hoja disminuyó (p< 0.05) al igual que el número de hojas por planta, el incremento en el rendimiento de vaina se dio con un aumento (p< 0.05) en el número de vainas por planta. El alto rendimiento de vaina permitió asegurar una resiembra natural de C. pumila. El rendimiento total varió de 9 a un poco más de 20 t ha-1. La conclusión es que con base al rendimiento total Crotalaria pumila puede ser una opción de especie forrajera.

Abstract Crotalaria pumila Ort. is a legume native to Mexico with little information about its forage potential based on its profile of accumulation during the summer, so a study was carried out with the aim of determining the total yield and the yield by component during 2021 to generate base information to decide regarding the possibility of the species as a forage species. In an area with spontaneous C. pumila, nine weekly samplings were carried out, in which we determined the total yield and yield by components: leaf, stem, flower, and pods, their proportional contribution to the total and number per plant and plant height. The statistical analysis was for a completely randomized design with three repetitions, the independent variable was the number of days elapsed in the summer. As the summer progressed, total and pod yields increased (p< 0.05), stem yield remained constant (p> 0.05), and leaf yield (p< 0.05) and the number of leaves per plant decreased, the increase in pod yield occurred with an increase (p< 0.05) in the number of pods per plant. The high pod yield made it possible to ensure a natural reseeding of C. pumila. The total yield ranged from 9 to just over 20 t ha-1. The conclusion is that based on the total yield, Crotalaria pumila can be a forage species option.

https://doi.org/10.29312/remexca.v14i7.3199

13.

A New Molecularly Imprinted Polymer for In-Tube SPME/UHPLC-MS/MS of Anandamide in Plasma Samples
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Pinto, Mônia A. L.

; Souza, Israel D. de

; Miranda, Luis Felippe C.

; Queiroz, Maria Eugênia C.

Journal of the Brazilian Chemical Society

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nov 2023, Volume 34 N. 11 Pages 1592 - 1600

Abstract: En | Text: En | PDF: En

Parkinson’s disease is a neurodegenerative disorder characterized by progressive loss of dopaminergic neurons. There is substantial evidence that the endocannabinoid system modulates the dopaminergic activity in the basal ganglia, a forebrain system that integrates cortical information to coordinate motor activity regulating signals. In this article, a fused-silica capillary with a molecularly imprinted polymer was developed for in-tube solid-phase microextraction of the endocannabinoid anandamide in plasma samples from Parkinson’s disease patients and further analysis by ultra-high-performance liquid chromatography with tandem mass spectrometry. The molecularly imprinted polymer capillary presented recognition sites with complementary shape, size, and functionality to anandamide. Scanning electron micrographs and Fourier transform infrared spectra illustrated the physical and chemical modification of the printed and non-printed capillary surface. The in-tube solid-phase microextraction ultra-high-performance liquid chromatography with tandem mass spectrometry method presented a linear range from 0.1 to 20 ng mL-1, precision with coefficient of variation values ranging from 1.2 to 13%, and relative standard deviation accuracy ranging from -3.6 to 7.5%. The method developed herein can adequately determine anandamide in plasma samples from Parkinson’s disease patients. By applying the standard addition approach, the anandamide plasmatic concentration in these samples was found to range from 0.2 to 0.4 ng mL-1.

https://doi.org/10.21577/0103-5053.20230074

14.

Maize-ruzigrass intercropping, nitrogen fertilization and plant density improve the performance of soybean grown in succession
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Coelho, Antonio E.

; Sangoi, Luis

; Sapucay, Moryb J. L. da C.

; Bratti, Felipe

; Debiasi, Henrique

; Franchini, Julio C.

; Garbelini, Luiz G.

; Balbinot Junior, Alvadi A.

Revista Brasileira de Engenharia Agrícola e Ambiental

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oct 2023, Volume 27 N. 10 Pages 764 - 771

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Second-crop maize-ruzigrass (Urochloa ruziziensis) intercropping, nitrogen (N) fertilization, and high maize plant densities enhance biomass production and N cycling, which may favor soybean growth and yield in the following season. This study aimed to assess the effects of second-crop maize-ruzigrass intercropping, N top dressing, and maize plant density on straw production in autumn/winter, N cycling, and agronomic performance of soybean crops grown in succession. Field experiments were installed in the 2018/2019 and 2019/2020 seasons using a randomized complete block design with split-split plots and four replications. The following factors were investigated: cropping system (sole maize and maize intercropped with ruzigrass), N top dressing (0 and 80 kg ha-1), and maize plant density (40, 60, 80, and 100 thousand plants ha-1). Maize-ruzigrass intercropping improved straw yield (2,365 kg ha-1) and N cycling (50 kg ha-1), and increased soybean yield in the following season (232 kg ha-1). N fertilization of maize increased soybean grain yield by 180 kg ha-1. Maize plant density did not influence the performance of succeeding soybean crops, regardless of the growing season.

RESUMO O consórcio milho segunda safra com braquiária ruziziensis (Urochloa ruziziensis), adubação com nitrogênio (N) e alta densidade de plantas do milho aumentam a produção de biomassa e a ciclagem de N, o que pode favorecer o crescimento e a produtividade da soja na safra seguinte. Este estudo teve como objetivo avaliar os efeitos do consórcio milho segunda safra com Brachiaria ruziziensis, aplicação de N em cobertura e densidade de plantas de milho sobre a produção de palha no outono/inverno, ciclagem de N e desempenho agronômico da soja em sucessão. Os experimentos de campo realizados nas safras 2018/2019 e 2019/2020, em delineamento de blocos completos casualizados com parcelas subdivididas e quatro repetições, investigaram os seguintes fatores: sistema de cultivo (milho solteiro e milho consorciado com Brachiaria ruziziensis), cobertura de N (0 e 80 kg ha-1) e densidade de plantas de milho (40, 60, 80 e 100 mil plantas ha-1). O consórcio milho-Brachiaria ruziziensis melhorou a produtividade de palha (2.365 kg ha-1) e a ciclagem de N (50 kg ha-1) e aumentou a produtividade de soja em sucessão (232 kg ha-1). A adubação nitrogenada do milho aumentou a produtividade de grãos de soja em 180 kg ha-1. A densidade de plantas do milho não influenciou o desempenho da soja cultivada em sucessão, independentemente da safra.

https://doi.org/10.1590/1807-1929/agriambi.v27n10p764-771

15.

Long-term oncological and surgical outcomes after Video Endoscopic Inguinal Lymphadenectomy (VEIL) in patients with penile cancer
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Tobias-Machado, Marcos

; Ornellas, Antonio A.

; Hidaka, Alexandre K.

; Medina, Luis G.

; Mattos, Pablo A. L.

; Besio, Ruben S.

; Abreu, Diego

; Castro, Pedro R.

; Nishimoto, Ricardo H.

; Astigueta, Juan

; Dourado, Aurus

; Machado, Roberto D.

; Magnabosco, Wesley J.

; Corona-Montes, Victor

; Villoldo, Gustavo M.

; Zampolli, Hamilton C.

; Taha, Anis

; Auad, Pericles R.

; Faria, Eliney F.

; Arantes, Paulo B. O.

; Tavares, Alessandro

; Nascimento, Francisco S. M. S.

; Brazão Jr., Eder S.

; Rocha, Maurício M.

; Costa, Walter H.

; Panico, Vinicius

; Reis, Leonardo O.

; Almeida-Carrera, Roberto J.

; Silva, Rafael C.

; Zequi, Stênio C.

; Calixto, José R. R.

; Sotelo, Rene

International braz j urol

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oct 2023, Volume 49 N. 5 Pages 580 - 589

Abstract: En | Text: En | PDF: En

ABSTRACT Objective: To report outcomes from the largest multicenter series of penile cancer patients undergoing video endoscopic inguinal lymphadenectomy (VEIL). Materials and Methods: Retrospective multicenter analysis. Authors of 21 centers from the Penile Cancer Collaborative Coalition-Latin America (PeC-LA) were included. All centers performed the procedure following the same previously described standardized technique. Inclusion criteria included penile cancer patients with no palpable lymph nodes and intermediate/high-risk disease and those with non-fixed palpable lymph nodes less than 4 cm in diameter. Categorical variables are shown as percentages and frequencies whereas continuous variables as mean and range. Results: From 2006 to 2020, 210 VEIL procedures were performed in 105 patients. Mean age was 58 (45-68) years old. Mean operative time was 90 minutes (60-120). Mean lymph node yield was 10 nodes (6-16). Complication rate was 15.7%, including severe complications in 1.9% of procedures. Lymphatic and skin complications were noted in 8.6 and 4.8% of patients, respectively. Histopathological analysis revealed lymph node involvement in 26.7% of patients with non-palpable nodes. Inguinal recurrence was observed in 2.8% of patients. 10y- overall survival was 74.2% and 10-y cancer specific survival was 84.8%. CSS for pN0, pN1, pN2 and pN3 were 100%, 82.4%, 72.7% and 9.1%, respectively. Conclusion: VEIL seems to offer appropriate long term oncological control with minimal morbidity. In the absence of non-invasive stratification measures such as dynamic sentinel node biopsy, VEIL emerged as the alternative for the management of non-bulky lymph nodes in penile cancer.

https://doi.org/10.1590/s1677-5538.ibju.2023.0065

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