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Abstract Introduction: In Mexico, cardiac rehabilitation (CR) as an interdisciplinary intervention with therapeutic impact in patients with heart disease is growing. There is the need to know actual conditions of CR in our country. Objectives: The objective of this National Registry is to follow-up those existing and new CR units in Mexico through the comparison between the two previous registries, RENAPREC-2009 and RENAPREC II-2015 studies. This is a descriptive study focused on diverse CR activities such as assistance training, and certification of health professionals, barriers, reference, population attended, interdisciplinarity, permanence over time, growth prospects, regulations, post-pandemic condition, integrative characteristics, and scientific research. Results: Data were collected from 45 CR centers in the 32 states, 75.5% are private practice units, 67% are new, 33% were part of RENAPREC II-2015, and 17 have continued since 2009. With a better distribution of CR units along the territory, the median reference of candidates for CR programs is 9% with a significant reduction into tiempo of enrollment to Phase II admission (19 ± 11 days). Regarding to previous registries, the coverance of Phases I, II, and III is 71%, 100%, and 93%, respectively; and a coverance increases in evaluation, risk stratification, and prescription, more comprehensive attendance and prevention strategies. Conclusions: CR in Mexico has grown in the past 7 years. Even there is still low reference and heterogeneity in specific processes, there are strengths such as interdisciplinarity, scientific professionalization of specialists, national diversification, and an official society that are consolidated over time.

https://doi.org/10.24875/acm.22000235

Economic indicators for cowpea cultivation under different irrigation depths
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Carvalho, Erika de O. T. de

; Costa, Deborah L. P.

; Luz, Denilson B. da

; Rua, Matheus L.

; Velame, Maria de L. A.

; Monteiro, Adrielle C.

; Vieira, Igor C. de O.

; Pinto, João V. de N.

; Fernandes, Gabriel S. T.

; Nunes, Hildo G. G. C.

; Souza, Paulo J. de O. P. de

; Santos, Marcos A. S. dos

Revista Brasileira de Engenharia Agrícola e Ambiental

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aug 2023, Volume 27 N. 8 Pages 618 - 624

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Cowpeas are of high socioeconomic importance in the North and Northeast regions of Brazil, whereby the search for alternatives that offer increased productivity with financial returns has become an important challenge. Thus, the objective of this study was to estimate and evaluate the economic indicators of cowpea production in 2015 and 2016 when applying drip irrigation to different depths. Irrigation treatments were based on crop evapotranspiration (ETc). The experiment was laid in a randomized complete blocks design with four treatments (0, 25, 50, and 100% ETc) and six replications. For economic analysis, the results were extrapolated to an area of one hectare. Productivity in 2015 was lower than that in 2016; however, in all treatments, it was always higher when plants were grown under adequate water supply. In both years, the total operating costs were higher under irrigation than under rainfed conditions. The 100% ETc treatment resulted in a greater number of cowpea bags (60 kg) produced and a higher gross revenue than the rainfed treatment by 17 bags ha-1, equivalent to 1,020 kg ha-1 (gross revenue US$ 711.48) and 16 bags ha-1, equivalent to 960 kg ha-1 (gross revenue US$ 867.12), in 2015 and 2016, respectively. Water deficit limited cowpea yield in both years but made cultivation economically unfeasible only in 2015.

RESUMO O feijão-caupi apresenta grande importância socioeconômica nas regiões Norte e Nordeste do Brasil, sendo que a busca por alternativas que ofereçam aumento de produtividade com retorno financeiro tem se tornado um importante desafio. Assim, o objetivo deste trabalho foi estimar e avaliar os indicadores econômicos da produção de feijão-caupi nos anos de 2015 e 2016 quando da aplicação de irrigação por gotejamento em diferentes lâminas. Os tratamentos de irrigação foram baseados na evapotranspiração da cultura (ETc). O experimento foi instalado em blocos ao acaso com quatro tratamentos (0, 25, 50 e 100% da ETc) e seis repetições. Para análise econômica, os resultados foram extrapolados para uma área de um hectare. A produtividade em 2015 foi inferior à de 2016; porém, em todos os tratamentos, a produtividade foi sempre maior quando as plantas foram cultivadas sob irrigação adequada. Em ambos os anos, os custos operacionais totais foram maiores sob irrigação do que sob condições de sequeiro. O tratamento 100% ETc resultou em maior número de sacas de feijão-caupi (60 kg) produzidas e receita bruta superior ao tratamento de sequeiro em 17 sacas ha-1, equivalente a 1.020 kg ha-1 (receita bruta US$ 711,48) e 16 sacas ha-1, equivalente a 960 kg ha-1 (receita bruta US$ 867,12), em 2015 e 2016, respectivamente. A deficiência hídrica limitou a produtividade do feijão-caupi nos dois anos, mas inviabilizou economicamente o cultivo apenas em 2015.

https://doi.org/10.1590/1807-1929/agriambi.v27n8p618-624

Thermal synthesis of NiMn2O4 spinel employing Mn oxide ores as starting material
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Nascimento, R. S.

; Figueira, B. A. M.

; F. Neto, E.

; Rabelo, A. A.

; Fernandez, O. J. C.

; Luz, P. T. S. da

; Costa, M. L. da

Cerâmica

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apr 2023, Volume 69 N. 390 Pages 154 - 160

Abstract: En | Text: En | PDF: En

Abstract It is described for the first time the synthesis and characterization of NiMn2O4 employing the Mn ores from the Amazon Region as starting material, instead of using commercial reagents as normally occurs in its synthesis process. Initially, an Mn ore was transformed into a single product, the octahedral layer (OL) manganese oxide with Ni2+ ions interlayer (Ni-OL-2), which was used as a matrix to obtain NiMn2O4 by thermal decomposition. The following techniques were used for characterization: X-ray diffraction (XRD), X-ray fluorescence spectroscopy (XRF), infrared spectroscopy (FTIR), thermal analysis (TG-DTA), scanning (SEM) and transmission (TEM) electron microscopies. The results revealed that Mn oxide ore composed of nsutite mineral transformed into a well-ordered crystalline Ni-OL-2 phase, after the hydrothermal and cation exchange process. The thermal decomposition of Ni-OL-2 produced the nickel manganite phase at 850 °C with spherical ball morphology and crystal sizes in the range of 200-300 nm.

https://doi.org/10.1590/0366-69132023693903436

Do biofertilizers affect nodulation ability and pod production in peanut genotypes?
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JULIÃO, ANA KELLY S.

; LUZ, LUCAS N. DA

; GADELHA, MARYSSOL T.

; OLIVEIRA, MATHEUS L.

; SILVEIRA, MARIA V.S.

; CASTRO, SAMUEL F.A.O.

; BARROS, LENIN P.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 N. 2 elocation e20201163

Abstract: En | Text: En | PDF: En

Abstract Peanuts are an important legume for the Northeastern Brazilian market, but their production in this region is low. The present study aimed to evaluate the effect of biofertilizer doses on peanut nodulation and production components, to define the best dose and genotype. The experiment was conducted in a completely randomized block design (DBC) in a 3 x 3 factorial arrangement, with three replications. The treatments consisted of two evaluation factors: 1) Fertilization via different doses of organic fertilizer applied to the substrate (D1 = 0mL; D2 = 500mL; and D3 = 1000mL); and 2) Peanut genotypes (BR-1, UNI43 and UNI08). The following traits were assessed: number of nodules per plant (NNP), number of mature pods (NVM), pod mass per plant (MVP), seed mass per plant (MSP) and root length (CR). Peanut production is affected by fertilization via bovine biofertilizer, mainly for the components NVM, MVP and MSP. Besides, nodulation is a likely conditioner. The 1000mL dose proved to be the best treatment for the traits analyzed, and UNI08 accession, the most responsive material.

https://doi.org/10.1590/0001-3765202220201163

Evaluation of the combined addition of aluminum lactate and calcium carbonate to alumina-based castables
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Consonni, L. B.

; Luz, A. P. da

; Pandolfelli, V. C.

Cerâmica

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jun 2021, Volume 67 N. 382 Pages 196 - 202

Abstract: En | Text: En | PDF: En

Abstract Calcium aluminate cement (CAC) can induce the development of high green mechanical strength to refractory castables in a short period of time (24 h). However, the production of this hydraulic binder is energy-intensive and releases a large content of carbon dioxide (CO2), which adds to global warming. Thus, aiming to develop an alternative binding system, this study investigated the combined addition of aluminum lactate (0.25-1.0 wt%) and calcium carbonate (3.2 wt%) to alumina castables. The prepared samples were analyzed using different experimental techniques, such as cold and hot modulus of rupture, hot elastic modulus, X-ray diffraction, and apparent porosity. According to the results, the lactate addition to the castables improved their green mechanical strength after drying at 110 °C/24 h (4.89 MPa) when compared to the one containing plain calcium carbonate (1.72 MPa). Furthermore, the carbonate-containing refractories presented cold and hot modulus of rupture similar or even superior to the castable containing cement in a wide temperature range (600-1500 °C).

https://doi.org/10.1590/0366-69132021673823104 185 downloads

Sensibilidad de Phytophthora infestans a extractos acuosos de Lippia origanoides y Origanum vulgare
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Burbano-David, Diana

; Lagos-Mora, Luz Estela

; Álvarez-Ordoñez, Sandra

; Chañag-Miramag, Harold Andrés

Agronomía Mesoamericana

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apr 2021, Volume 32 N. 1 Pages 149 - 162

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Introducción. Phytophthora infestans (Mont.) de Bary, es considerado el agente causal de una de las enfermedades más devastadoras y limitantes de cultivos de papa (Solanum tuberosum L.) en el departamento de Nariño (Colombia). Esta enfermedad es controlada con fungicidas sistémicos, los cuales tienen un impacto negativo sobre el ambiente e incrementan los costos de producción, sin embargo, existen alternativas como los denominados fungicidas orgánicos, los cuales se basan en el uso de extractos de plantas con propiedades antifúngicas para el control del patógeno. Objetivo. Evaluar extractos acuosos de O. vulgare y L. origanoides para el control de P. infestans bajo condiciones in vitro. Materiales y métodos. La presente investigación se desarrolló en el Laboratorio de microbiología del grupo de investigación en Genética y Evolución de Organismos Tropicales (GENPAT) de la Universidad de Nariño, durante el año 2017. Se evaluó la sensibilidad in vitro de P. infestans frente a extractos de las especies botánicas Lippia origanoides (orégano silvestre) y Origanum vulgare (orégano común). Resultados. Los datos obtenidos permitieron el establecimiento de la línea base de comportamiento de las poblaciones del patógeno frente a los compuestos evaluados, hubo una reducción gradual tanto en el crecimiento como en la esporulación cuando se empleó el extracto de Lippia origanoides y que este mismo tiene la capacidad de controlar el patógeno en concentraciones de 100 mg ml-1. Con el empleo de Origanum vulgare los aislamientos de P. infestans presentaron diferentes niveles de sensibilidad al compuesto con bajos niveles de inhibición y altos valores de EC-50. Conclusión. Los aislamientos de P. infestans presentaron diferentes niveles de sensibilidad frente a los dos extractos acuosos evaluados, con una reducción del 100 % en la inhibición del crecimiento radial y la esporulación a partir de la concentración de 100 mg ml-1, cuando se empleó el extracto de L. origanoides.

Abstract Introduction. Phytophthora infestans (Mont.) De Bary, is considered the causative agent of one of the most devastating and limiting diseases of potato crops (Solanum tuberosum L.) in the Nariño department (Colombia). This disease is controlled using systemic fungicides, which have a negative impact on the environment and increase production costs, however, there are alternatives like the organic fungicides, which are based on the use of plant extracts with anti-fungal properties for the control of the pathogen. Objective. To evaluate aqueous extracts of O. vulgare and L. origanoides for the control of P. infestans under in vitro conditions. Materials and methods. This research was carried out in the Microbiology laboratory of the research group in Genetics and Evolution of Tropical Organisms (GENPAT) of the Universidad de Nariño, during 2017. The in vitro sensitivity of P. infestans against extracts of botanical species Lippia origanoides (wild oregano) and Origanum vulgare (common oregano) was evaluated. Results. The data obtained allowed the establishment of the base line of behavior of the pathogen populations against the evaluated compounds, there was a gradual reduction in both the growth and sporulation when the Lippia origanoides extract was used and that this same has the capacity to control the pathogen in concentrations of 100 mg ml-1. With the use of Origanum vulgare the P. infestans isolates showed different levels of sensitivity to the compound with low levels of inhibition and high values of EC-50. Conclusion. The P. infestans isolates presented different levels of sensitivity against the two aqueous extracts evaluated, with a reduction of 100 % in the inhibition radial growth and sporulation from the concentration of 100 mg ml-1, when the extract was used of L. origanoides.

https://doi.org/10.15517/am.v32i1.40573

Evaluación del efecto antifúngico de metabolitos de cultivos bioprotectores: aplicación en derivados lácteos
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OLIVARES-TENORIO, MARY LUZ

; KLOTZ-CEBERIO, BERNADETTE

Biotecnología en el Sector Agropecuario y Agroindustrial

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dec 2020, Volume 18 N. 2 Pages 15 - 25

Abstract: Es En Pt | Text: Es En Pt | PDF: Es | PDF: En | PDF: Pt

RESUMEN Para garantizar la inocuidad y la vida útil de muchos alimentos procesados, la industria de alimentos ha hecho uso de conservantes artificiales que cada vez son más cuestionados y reemplazados por alternativas naturales como son los cultivos bioprotectores. Las bacterias ácido lácticas (BAL) y ácido propiónicas (BAP) son los grupos bacterianos más reconocidos por su acción antimicrobiana sobre bacterias patógenas y en menor grado sobre hongos. El presente estudio evaluó la funcionalidad antifúngica de tres cepas de Lactobacillus casei, tres de L. rhamnosus y tres de Propionibacterium freudenreichii sobre aislados de Mucor circinelloides y Geotrichum candidum y el efecto de factores como la pasterización (74°C, 3 min) y el medio de crecimiento de los cultivos bioprotectores (leche y BRFS) sobre esta. Los ensayos reto con cultivos bioprotectores sin y con tratamiento térmico realizados por triplicado en modelos lácteos (agar yogur) arrojaron los siguientes resultados de acuerdo con una escala cualitativa de crecimiento fúngico: (1) diferentes cepas de BAL y BAP divergieron en funcionalidad bioprotectora, (2) el tratamiento térmico eliminó o redujo sustancialmente el poder antifúngico de los cultivos con la excepción de P. freudenreichii subsp. shermanii BPL0082 y (3) los cultivos desarrollados en medio BRFS presentaron mayor capacidad de inhibición.

ABSTRACT To guarantee the food safety and shelf life of many processed food products, the food industry has made use of artificial preservatives that are each time more questioned and replaced by natural alternatives such as bioprotective cultures. Lactic acid bacteria (LAB) and propionic acid bacteria (PAB) are bacterial groups recognized by their antimicrobial action against bacterial pathogens but their activity against fungi is lesser known. The present study evaluated the antifungal functionality of three strains Lactobacillus casei, three of L. rhamnosus, and three of Propionibacterium freudenreichii against isolates of Mucor circinelloides and Geotrichum candidum and the effect of factors like pasteurization (74°C, 3 min) and culture media (milk and BRFS) on it. The challenge assays in dairy models (yogurt agar) produced the following results in accordance to a qualitative score scale of fungal growth: (1) different strains of LAB and PAB differed in the bioprotective functionality, (2) the heat treatment eliminated or reduced substantially the antifungal power with the exception of P. freudenreichii subsp. shermanii BPL0082, and (3) the cultures developed in media BRFS exhibited greater inhibition capacity.

RESUMO Para garantir a inocuidade e a vida útil de muitos alimentos processados, a indústria alimentícia faz uso de conservantres artificiais, que vêm sendo cada vez mais questionados e substituídos por alternativas naturais, como é o caso das culturas bioprotetoras. As bactérias ácido-láticas (BAL) e ácido-propiônicas (BAP) constituem os grupos bacterianos mais reconhecidos por sua ação antimicrobiana sobre bactérias patogênicas e, em menor grau, sobre os fungos. O presente estudo avaliou a funcionalidade antifúngica de três cepas de Lactobacillus casei, três de L. rhamnosus e três de Propionibacterium freudenreichii sobre isolados de Mucor circinelloides e Geotrichum candidum, bem como o efeito de fatores como a pasteurização (74°C, 3 min) e o meio de crescimento das culturas bioprotetoras (leite e BRFS) sobre ela. Os ensaios de desafio com culturas bioprotetoras com e sem tratamento térmico realizados por triplicado em modelos lácteos obtiveram os seguintes resultados de acordo com uma escala qualitativa de crescimento fúngico: (1) diferentes cepas de BAL e BAP divergem quanto à função bioprotetora, (2) o tratamento térmico eliminou ou reduziu o poder antifúngico com exceção da P. freudenreichii subsp. shermanii BPL0082 e (3) o culturas desenvolvidas em meio BRFS exibiu maior capacidade de inibição.

https://doi.org/10.18684/bsaa.v18n2.114 4692 downloads

Calcination thermokinetics of three Brazilian limestones
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Silveira, M. A. C. W. da

; Luz, J. A. M. da

; Faria, G. L. de

; Coutinho, F. M. P.

Cerâmica

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sep 2020, Volume 66 N. 379 Pages 297 - 306

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Os produtores de calcário e cal inadvertidamente geram uma quantidade considerável de material fino durante o processamento, tornando-se um grande passivo ambiental. Quanto ao processo de calcinação, a indústria ressente-se de dados experimentais da lei de Arrhenius, como fator de frequência aparente e energia de ativação, referentes aos calcários brasileiros. O conhecimento desses parâmetros termocinéticos pode diminuir o consumo energético durante o processo de calcinação industrial, além de permitir o uso de finos de calcário em outras aplicações industriais. Este trabalho teve como objetivo caracterizar três calcários brasileiros, concentrando-se em sua calcinação em escala laboratorial, usando método quase isotérmico e dinâmico (termogravimétrico) para estimar seus parâmetros cinéticos. O método dinâmico permitiu estimar a energia de ativação de cada calcário através das regiões de alta taxa de calcinação. Porém, os parâmetros cinéticos estimados pelo método quase isotérmico resultaram em valores mais baixos, indicando a necessidade de controlar a atmosfera do reator.

Abstract Limestone and lime producers inadvertently generate a considerable amount of fine material during their processing, consequently becoming a great environmental liability. As far as the industrial calcination process is concerned, there is still a need for an experimental survey on Arrhenius law parameters, as apparent frequency factor and activation energy, referring to Brazilian limestones. The knowledge of these thermokinetic parameters may contribute to energy saving during the industrial calcination process, as well as for allowing the use of limestone fines in other industrial applications. This study aimed to characterize three Brazilian limestones by focusing on their laboratory-scale calcination using both quasi-isothermal and non-isothermal methods to estimate their kinetic parameters. The non-isothermal method (by thermogravimetric analyses) allowed estimating the activation energy for each limestone through the high calcination rate ranges. However, the kinetic parameters estimated by the quasi-isothermal method turned out in lower values, indicating the need to control the reactor atmosphere.

https://doi.org/10.1590/0366-69132020663792910 760 downloads

Clinical and Epidemiological Characteristics of Patients Diagnosed with COVID-19 in a Tertiary Care Center in Mexico City: A Prospective Cohort Study
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Ortiz-Brizuela, Edgar

; Villanueva-Reza, Marco

; González-Lara, María F.

; Tamez-Torres, Karla M.

; Román-Montes, Carla M.

; Díaz-Mejía, Bruno A.

; Pérez-García, Esteban

; Olivas-Martínez, Antonio

; Rajme-López, Sandra

; Martinez-Guerra, Bernardo A.

; León-Cividanes, Nereyda A. de

; Fernández-García, Oscar A.

; Guerrero-Torres, Lorena

; Torres-González, Lorena

; Carrera-Patiño, Fabián A.

; Corral-Herrera, Ever A.

; Hernández-Alemón, Aldo N.

; Tovar-Vargas, Maria de los Ángeles

; Serrano-Pinto, Yamile G.

; Espejo-Ortiz, Cristian E.

; Morales-Ortega, María de la L.

; Lozano-Cruz, Óscar A.

; Cárdenas-Fragoso, José L.

; Vidal-Mayo, José de J.

; Hernández-Gilsoul, Thierry

; Rivero-Sigarroa, Eduardo

; Domínguez-Cherit, Guillermo

; Cervantes-Villar, Luz E.

; Ramos-Cervantes, Maria del P.

; Ibarra-González, Violeta

; Calva-Mercado, Juan J.

; Sierra-Madero, Juan G.

; López-Íñiguez, Álvaro

; Ochoa-Hein, Eric

; Crabtree-Ramírez, Brenda E.

; Galindo-Fraga, Arturo

; Guerrero-Almeida, María de L.

; Ruiz-Palacios, Guillermo M.

; Gulías-Herrero, Alfonso

; Sifuentes-Osornio, José

; Kershenobich-Stalnikowitz, David

; Ponce-de-León, Alfredo

Revista de investigación clínica

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jun 2020, Volume 72 N. 3 Pages 165 - 177

Abstract: En | Text: En | PDF: En

ABSTRACT Background: Regional information regarding the characteristics of patients with coronavirus disease (COVID)-19 is needed for a better understanding of the pandemic. Objective: The objective of the study to describe the clinical features of COVID-19 patients diagnosed in a tertiary-care center in Mexico City and to assess differences according to the treatment setting (ambulatory vs. hospital) and to the need of intensive care (IC). Methods: We conducted a prospective cohort, including consecutive patients with COVID-19 from February 26, 2020 to April 11, 2020. Results: We identified 309 patients (140 inpatients and 169 outpatients). The median age was 43 years (interquartile range, 33-54), 59.2% men, and 18.6% healthcare workers (12.3% from our center). The median body mass index (BMI) was 29.00 kg/m2 and 39.6% had obesity. Compared to outpatients, inpatients were older, had comorbidities, cough, and dyspnea more frequently. Twenty-nine (20.7%) inpatients required treatment in the IC unit (ICU). History of diabetes (type 1 or 2) and abdominal pain were more common in ICU patients compared to non-ICU patients. ICU patients had higher BMIs, higher respiratory rates, and lower room-air capillary oxygen saturations. ICU patients showed a more severe inflammatory response as assessed by white blood cell count, neutrophil and platelet count, C-reactive protein, ferritin, procalcitonin, and albumin levels. By the end of the study period, 65 inpatients had been discharged because of improvement, 70 continued hospitalized, and five had died. Conclusions: Patients with comorbidities, either middle-age obese or elderly complaining of fever, cough, or dyspnea, were more likely to be admitted. At admission, patients with diabetes, high BMI, and clinical or laboratory findings consistent with a severe inflammatory state were more likely to require IC.

https://doi.org/10.24875/ric.20000211 533 downloads

10.

Uso de partículas magnéticas en la purificación de anticuerpos IgM contra Taenia solium
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Perez, L. Agueda

; Castillo, Yesenia

; Espinoza, Cindy

; Toribio, Luz M.

; Santos, Yesica

; Martel, Kevin S.

; Wilkins, Patricia P.

; Bustos, Javier A.

; García, Hector H.

; Castro-Sesquen, Yagahira E.

Revista Peruana de Medicina Experimental y Salud Pública

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mar 2020, Volume 37 N. 1 Pages 104 - 109

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT The use of L protein coupled magnetic particles for the concentration and purification of immunoglobulin M (mIgM) monoclonal antibodies against Taenia solium was evaluated. Three concentration methods and different elution times were evaluated and the ratio of particles to the ratio of mIgM was optimized. It is demonstrated that: 1) with the use of magnetic particles, a previous concentration of mIgM is not required, which reduces the manipulation of the antibodies and improves the recovery, 2) the use of a binding buffer can be omitted, since the pH of most cell culture supernatants are neutral, and 3) longer elution times (~ 45 minutes) are needed to increase recovery to a level greater than 80%. The study demonstrates that the use of L protein-coupled magnetic particles is a simple and efficient tool for mIgM concentration and purification.

RESUMEN Se evaluó el uso de partículas magnéticas acopladas a proteína L para la concentración y purificación de anticuerpos monoclonales inmunoglobulina M (mIgM) contra Taenia solium. Se evaluaron tres métodos de concentración y diferentes tiempos de elución y se optimizó la proporción de partículas a la proporción de mIgM. Demostramos que: 1) con el uso partículas magnéticas no se requiere de una concentración previa de mIgM, lo que disminuye la manipulación de los anticuerpos y mejora la recuperación, 2) se puede omitir el uso de un tampón de unión, ya que el pH de la mayoría de los sobrenadantes de cultivo celular son neutros, y 3) se necesitan tiempos de elución más largos (~45 minutos) para aumentar la recuperación a un nivel mayor a 80%. El estudio demuestra que el uso de partículas magnéticas acopladas a proteína L es una herramienta simple y eficiente para la concentración y purificación de mIgM.

https://doi.org/10.17843/rpmesp.2020.371.4628

11.

REUSE OF COLLAGEN AND HYDROXYAPATITE FROM THE WASTE PROCESSING OF FISH TO PRODUCE POLYETHYLENE COMPOSITES
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Boaventura, Túlio P.

; Peres, Anderson M.

; Gil, Viviane S. B.

; Gil, Camila S. B.

; Oréfice, Rodrigo L.

; Luz, Ronald K.

Química Nova

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feb 2020, Volume 43 N. 2 Pages 168 - 174

Abstract: En | Text: En | PDF: En

In recent years, the increase in the generation of waste (e.g., bones and skin) from the processing of fish-based food related to the increase in fish consumption and the accumulation of synthetic polymers in the environment are causing major environmental concerns. Hydroxyapatite (HA) extracted from the bones and collagen from the skin of fish processing residues can be combined with synthetic polymers to maximize the use of waste material derived from fish while simultaneously reducing the use of synthetic polymers. Hence, in this study, collagen and HA were extracted from the carcasses of Lophiosilurus alexandri, and the obtained materials were reused to prepare low-density polyethylene (LDPE) blends and composites. The integrity of the triple helix structure of collagen was confirmed by FTIR and DSC showing that there was no collagen denaturation during its extraction and sample processing. The results indicated that the material produced by the incorporation of collagen into LDPE compatibilized with polyethylene-graft-maleic anhydride exhibits good mechanical and surface properties for use as a support material for ornamental articles, among other applications.

https://doi.org/10.21577/0100-4042.20170475 432 downloads

12.

Uso de partículas magnéticas en la purificación de anticuerpos IgM contra Taenia solium
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Perez, L. Agueda

; Castillo, Yesenia

; Espinoza, Cindy

; Toribio, Luz M.

; Santos, Yesica

; Martel, Kevin S.

; Wilkins, Patricia P.

; Bustos, Javier A.

; García, Hector H.

; Castro-Sesquen, Yagahira E.

Revista Peruana de Medicina Experimental y Salud Publica

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jan 2020, Volume 37 N. 1 Pages 104 - 109

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

https://doi.org/10.17843/rpmesp.2020.371.4628

13.

Metodología para la elaboración de la Guía de práctica clínica
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Torres-Tamayo, Margarita

; Zurita-Cruz, Jessie N.

; Aguilar-Herrera, Blanca E.

; Miranda-Lora, América L.

; Calzada-León, Raúl

; Rivera-Hernández, Aleida J.

; Morales-Pérez, Marco A.

; Padrón-Martínez, Miriam M.

; Ruiz-Reyes, María L.

; García-Morales, Leticia M.

; Barrón-Uribe, Consuelo

; Arguinzoniz-Valenzuela, Sletza L.

; Torres-Castañeda, Mayra C.

; Lizárraga-Paulin, Lorena

; Núñez-Hernández, Jorge A.

; Cornejo-Barrera, Judith

; Vidal-González, María T.

; Martínez-Alvarado, María R.

; Nishimura-Meguro, Elisa

; Bravo-Ríos, Luz E.

; Garrido-Magaña, Eulalia P.

; Orozco-Morales, José A.

; Medina-Bravo, Patricia G.

; Coyote-Estrada, Ninel

; Castilla-Peón, M. Fernanda

Boletín médico del Hospital Infantil de México

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2020, Volume 77 Suppl. 1 Pages 1 - 6

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Background: The Mexican Society of Pediatric Endocrinology presents recommendations for the diagnosis and treatment of precocious puberty (PP), a condition defined as the development of sexual characteristics due to an increase in pituitary gonadotropin secretion before 8 or 9 years of age in girls and boys, respectively. Methods: Three systematic reviews were conducted: controlled clinical trials on interventions for PP treatment, diagnostic tests, and observational studies on the long-term effects of PP. The quality evaluation and data extraction from the studies were conducted by two independent reviewers. The Scottish Intercollegiate Guidelines Network and the Oxford Center for Evidence-Based Medicine systems were used for grading the quality of evidence for recommendations on intervention and diagnosis, respectively. Recommendations were submitted to a consensus by a Delphi method and were validated by another 143 certified pediatric endocrinologists through an online questionnaire. Results: The group generated 12 recommendations on the diagnosis of PP, seven on the diagnosis of secondary causes of PP, eight on interventions for inhibition of puberty, five on other interventions for PP treatment, and 14 for the monitoring and follow-up of these patients. The online questionnaires submitted to certified pediatric endocrinologists showed more than 90% of approval for each one of the recommendations. Conclusions: Although a high degree of consensus for the recommendations for diagnosis, treatment, and monitoring of PP among pediatric endocrinologists was achieved, most of these recommendations showed a low level of evidence.

Resumen Introducción: La Sociedad Mexicana de Endocrinología Pediátrica presenta recomendaciones para el diagnóstico y el tratamiento de la pubertad precoz (PP), condición definida como el desarrollo de caracteres sexuales por incremento en la secreción hipofisiaria de gonadotropinas antes de los 8 años en las niñas y de los 9 años en los niños. Métodos: Se realizaron tres revisiones sistemáticas de ensayos clínicos controlados sobre intervenciones para el tratamiento de la PP, pruebas diagnósticas y estudios observacionales sobre efectos a largo plazo de la PP. La evaluación de la calidad de los estudios y la extracción de datos se realizó por pares. La evidencia se graduó con el sistema de la Scottish Intercollegiate Guidelines Network (SIGN) y del Oxford Centre for Evidence-Based Medicine (OCEBM) para las recomendaciones sobre la intervención y el diagnóstico, respectivamente. Las recomendaciones generadas se sometieron a un consenso por el método Delphi y fueron validadas por otros 143 endocrinólogos pediatras certificados mediante un cuestionario en línea. Resultados: Mediante consenso se generaron 12 recomendaciones para el diagnóstico de PP, siete sobre diagnóstico de causas secundarias de PP, ocho sobre intervenciones para inhibición de la pubertad, cinco sobre otras intervenciones en PP y 14 para la monitorización del tratamiento y el seguimiento de estos pacientes. Se obtuvo más del 90% de aprobación para cada una de las recomendaciones por el grupo de endocrinólogos certificados que respondieron el cuestionario en línea. Conclusiones: Si bien se logró un alto grado de consenso para las recomendaciones para el diagnóstico, el tratamiento y la monitorización de la PP entre los endocrinólogos pediatras, el nivel de evidencia para la mayoría de estas recomendaciones resultó bajo.

https://doi.org/10.24875/bmhim.20000083 411 downloads

14.

Inhibición de la pubertad
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Torres-Tamayo, Margarita

; Zurita-Cruz, Jessie N.

; Aguilar-Herrera, Blanca E.

; Miranda-Lora, América L.

; Calzada-León, Raúl

; Rivera-Hernández, Aleida J.

; Morales-Pérez, Marco A.

; Padrón-Martínez, Miriam M.

; Ruiz-Reyes, María L.

; García-Morales, Leticia M.

; Barrón-Uribe, Consuelo

; Arguinzoniz-Valenzuela, Sletza L.

; Torres-Castañeda, Mayra C.

; Lizárraga-Paulin, Lorena

; Núñez-Hernández, Jorge A.

; Cornejo-Barrera, Judith

; Vidal-González, María T.

; Martínez-Alvarado, María R.

; Nishimura-Meguro, Elisa

; Bravo-Ríos, Luz E.

; Garrido-Magaña, Eulalia P.

; Orozco-Morales, José A.

; Medina-Bravo, Patricia G.

; Coyote-Estrada, Ninel

; Castilla-Peón, M. Fernanda

Boletín médico del Hospital Infantil de México

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2020, Volume 77 Suppl. 1 Pages 19 - 25

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract The Mexican Society of Pediatric Endocrinology developed a clinical practice guide for the diagnosis and treatment of precocious puberty. This document presents recommendations related to the interventions for the inhibition of central precocious puberty. The detailed description of the methodology for the development of this guide and the grading system, as well as the synthesis of the evidence on which it is based can be consulted in this same supplement.

Resumen La Sociedad Mexicana de Endocrinología Pediátrica elaboró una guía de práctica clínica para el diagnóstico y el tratamiento de la pubertad precoz. Este documento presenta recomendaciones relacionadas con las intervenciones para inhibir la pubertad precoz central. La descripción detallada de la metodología para el desarrollo de esta guía y del sistema de gradación, así como la síntesis de la evidencia en la que se basa, pueden consultarse en este mismo suplemento.

https://doi.org/10.24875/bmhim.20000085 455 downloads

15.

Diagnóstico de pubertad precoz
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Miranda-Lora, América L.

; Torres-Tamayo, Margarita

; Zurita-Cruz, Jessie N.

; Aguilar-Herrera, Blanca E.

; Calzada-León, Raúl

; Rivera-Hernández, Aleida J.

; Morales-Pérez, Marco A.

; Padrón-Martínez, Miriam M.

; Ruiz-Reyes, María L.

; García-Morales, Leticia M.

; Barrón-Uribe, Consuelo

; Arguinzoniz-Valenzuela, Sletza L.

; Torres-Castañeda, Mayra C.

; Lizárraga-Paulin, Lorena

; Núñez-Hernández, Jorge A.

; Cornejo-Barrera, Judith

; Vidal-González, María T.

; Martínez-Alvarado, María R.

; Nishimura-Meguro, Elisa

; Bravo-Ríos, Luz E.

; Garrido-Magaña, Eulalia P.

; Orozco-Morales, José A.

; Medina-Bravo, Patricia G.

; Coyote-Estrada, Ninel

; Castilla-Peón, M. Fernanda

Boletín médico del Hospital Infantil de México

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2020, Volume 77 Suppl. 1 Pages 7 - 14

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract The Mexican Society of Pediatric Endocrinology developed a clinical practice guide for the diagnosis and treatment of precocious puberty. This document presents recommendations related to the diagnosis of precocious puberty. The detailed description of the methodology for the development of this guide and the grading system, as well as the synthesis of the evidence on which it is based can be accessed in this same supplement.

Resumen La Sociedad Mexicana de Endocrinología Pediátrica elaboró una guía de práctica clínica para el diagnóstico y el tratamiento de la pubertad precoz. Este documento presenta recomendaciones relacionadas con el diagnóstico de pubertad precoz. La descripción detallada de la metodología para el desarrollo de esta guía y del sistema de gradación, así como la síntesis de la evidencia en la que se basa, pueden consultarse en este suplemento.

https://doi.org/10.24875/bmhim.20000075 1249 downloads

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