Abstract Objective: The objective of this study was to investigate the effects of activity-based locomotor training (ABLT) on motor function and walking ability in children with spinal cord injury (SCI). Materials and methods: The Chinese National Knowledge Infrastructure, WanFang, VIP, PubMed, and Web of Science databases were searched for related studies, with two reviewers subsequently evaluating the literature quality using the Cochrane Handbook. Results: A total of 11 studies were eligible, while only one met the ABLT standard program criteria. Overall, ABLT significantly improved the lower limb motor function, increased walking speed and distance, and improved the daily living ability of children with SCI. Conclusions: The ABLT strategy is of great significance to the motor function and walking ability of children with SCI. At present, there exist few studies on the application of ABLT for pediatric SCI. Further control studies with a larger sample size are required to improve the ABLT program guidelines for children with SCI.

Resumen Objetivo: Discuta el impacto del entrenamiento ejercicio basado en la actividad en la lesión de la médula espinal en la función de movimiento de los niños y la capacidad de caminar. Materials y métodos: Según China Zhiwang, Wanfang, VIP, PubMed, Science Network y otros documentos relacionados como fuente de datos. Dos revisores usan calidad de evaluación manual de Cochrane. Resultados: Un total de 11 estudios cumplen con las condiciones. Solo hay un estudio que cumple con los proyectos estándar de ABLT. General, ABLT mejora significativamente la función de los niños con lesiones de la médula espinal, aumenta la velocidad y la distancia de caminar y mejora la capacidad de la vida diaria. Conclusión: La estrategia ABLT es de gran importancia para la función de movimiento de los niños de la médula espinal y la capacidad de caminar. En la actualidad, ABLT tiene menos investigación en lesión pediátrica de la médula espinal. Es necesario mostrar la cantidad de muestra y controlar la investigación para mejorar las pautas del plan ABLT para el daño de la médula espinal a los niños.

RESUMO Introdução O acompanhamento psicológico e a intervenção psicológica dos jogadores de basquete tem sido foco de atenção no treinamento esportivo. No basquetebol, os jogadores consomem muita energia física e também muita energia mental. A tensão psicológica e as alterações emocionais fazem com que os jogadores de basquetebol restrinjam suas habilidades técnicas. Objetivo Analisar a relação entre a fadiga mental induzida pelo esporte e as emoções pré-jogo nos jogadores de basquete. Métodos Este trabalho seleciona 40 jogadores de basquetebol como voluntários de pesquisa. Utilizou-se um questionário de pesquisa e estatísticas matemáticas para analisar a relação entre a fadiga mental dos jogadores de basquetebol, o nível de treinamento e as emoções pré-jogo. Este trabalho analisa a relação entre a qualidade psicológica e o nível de treinamento dos jogadores de basquetebol. Resultados Houve correlações significativas entre as várias dimensões da fadiga mental induzida pelo esporte e a emoção no pré-jogo dos jogadores de basquete. A autoconfiança dos jogadores de basquetebol antes do jogo é afetada principalmente pela competência dos jogos de treinamento e pelo consumo excessivo de energia. Conclusão A qualidade psicológica dos jogadores de basquetebol está significativamente correlacionada com o nível de treinamento. Os atletas devem prestar atenção ao treinamento de qualidade psicológica nas competições esportivas. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

ABSTRACT Introduction Psychological monitoring and psychological intervention of basketball players has been a focus of attention in sports training. In basketball, players consume much physical energy and also much mental energy. Psychological tension and emotional changes cause basketball players to restrict their technical abilities. Objective Analyze the relationship between sport-induced mental fatigue and pre-game emotions in basketball players. Methods This paper selects 40 basketball players as research volunteers. A survey questionnaire and mathematical statistics were used to analyze the relationship between basketball players’ mental fatigue, training level, and pre-game emotions. This paper analyzes the relationship between psychological quality and training level of basketball players. Results There were significant correlations between various dimensions of sport-induced mental fatigue and basketball players’ pregame emotions. Basketball players’ pre-game self-confidence is mainly affected by training game competence and excessive energy consumption. Conclusion The psychological quality of basketball players is significantly correlated with the level of training. Athletes should pay attention to psychological quality training in sports competitions. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción El acompañamiento psicológico y la intervención psicológica de los jugadores de baloncesto han sido objeto de atención en el entrenamiento deportivo. En el baloncesto, los jugadores consumen mucha energía física y también mucha energía mental. La tensión psicológica y los cambios emocionales hacen que los jugadores de baloncesto limiten sus habilidades técnicas. Objetivo Analizar la relación entre la fatiga mental inducida por el deporte y las emociones previas al partido en jugadores de baloncesto. Métodos Este trabajo selecciona a 40 jugadores de baloncesto como voluntarios para la investigación. Para analizar la relación entre la fatiga mental de los jugadores de baloncesto, el nivel de entrenamiento y las emociones previas al partido se utilizó un cuestionario de encuesta y estadísticas matemáticas. Este artículo analiza la relación entre la calidad psicológica y el nivel de entrenamiento de los jugadores de baloncesto. Resultados Hubo correlaciones significativas entre las diversas dimensiones de la fatiga mental inducida por el deporte y las emociones previas al partido de los jugadores de baloncesto. La autoconfianza de los jugadores de baloncesto antes del partido se ve afectada principalmente por la competencia del juego de entrenamiento y el consumo excesivo de energía. Conclusión La calidad psicológica de los jugadores de baloncesto está significativamente correlacionada con el nivel de entrenamiento. Los deportistas deben prestar atención a la calidad psicológica del entrenamiento en las competiciones deportivas. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Abstract Due to chronic alcohol consumption, alcoholic liver disease (ALD) has become the second most common liver disease in the world. The study was to explore the effects of consuming different amounts of various alcoholic beverages on the liver of mice. Results showed wine contained far more polyphenols and organic acids than beer and Chinese Baijiu. When daily alcohol consumption was low (less than 100 mL/60 kg/day), the liver and serum indicators of mice treated with alcohol, beer, Chinese Baijiu, and wine were not significantly different from those in saline group. At 250 mL/60 kg/day, beer used significantly damaged the liver and caused adverse changes in serum components, while wine did not cause changes in serum, indicating that it may cause less damage due to the existence of a large number of polyphenols. When the daily consumption exceeded 500 mL/60 kg/day, all four alcoholic beverages significantly increased serum markers of hepatic injury and decreased serum total antioxidant capacity and liver antioxidant enzyme levels, resulting in liver fat accumulation and serious pathological damage. At this level, beer used caused the most damage and wine the least. This study explores the appropriate drinking amount of different alcoholic beverages and provides a scientific reference for alcohol consumption.

ABSTRACT Candida is one of the most frequent pathogens of bloodstream infections, which is associated with high morbidity and mortality rates. Rapid immunological detection methods are essential in the early diagnosis of candidemia. Anti-mannan is one of host-derived biomarkers against cell wall components of Candida. We conducted this study to evaluate the diagnostic performance of two anti-mannan assays (IgM, IgG) for candidemia through the analysis of 40 candidemia patients, 48 participants with Candida colonization and 213 participants with neither Candida colonization nor Candida infections (13 patients with other bloodstream infections, 145 hospitalized patients and 55 healthy controls). The performance of the two assays were evaluated by calculating their sensitivity and specificity. The sensitivity ranged from 0.78 to 0.80 for the IgM assay and 0.68 to 0.75 for the IgG assay. The specificity ranged from 0.97 to 0.98 for the IgM assay and 0.91 to 0.94 for the IgG assay. The diagnostic performance of the anti-mannan IgM assay was better than that of IgG, with higher sensitivity and specificity. Combining the two assays (positive results of single or both assays are both considered as positive) could improve the sensitivity up to 0.93 (0.79-0.98) and only slightly reduce the specificity (0.93(0.89-0.95)). The anti-mannan IgM, IgG assays are rapid and cost-effective assays that may be probably useful in the diagnosis of candidemia.

Abstract The effect of high Cu2+ stress on fermentation performance and copper biosorption of Saccharomyces cerevisiae BH8 during wine fermentation was investigated in this paper. Under high Cu2+ stress, the cell growth and survival rate of yeast BH8 were inhibited. With different copper concentration, the effect on cell growth of yeast BH8 was different. What’s more, high Cu2+ stress could inhibit the fermentation property, alcohol production and reducing sugar utilization of yeast BH8 in Chardonnay grape must, but not significant. And the trend of the copper ion concentration falling down was consistent with the trend of yeast growth rising up, meanwhile the copper content was still much less than the initial concentration after fermentation. In addition, yeast BH8 showed a good fermenting property and copper removal ability under high Cu2+ stress. These results could provide certain reference and some new data for the wine industry to face the copper pollution risk.

ABSTRACT We first present a cost-effective approach to simultaneously dispose of rutaceae plant waste (the discarded peels of orange, finger citron, pomelo and lemon) to yield biomass nanoporous carbons (BNCs). The adsorption of orange II (OII) and acid chrome blue K (ACBK) from aqueous solutions in single and binary dye systems by four types of BNCs were studied in a batch adsorption system. The adsorption studies include both equilibrium adsorption isotherms and kinetics. Four adsorption models for predicting the multicomponent equilibrium sorption isotherms have been compared in order to determine which one is the best fit model to predict or correlate binary adsorption data. The kinetic data were well described by the pseudo-second-order kinetic model. It was found that the adsorption capacity of orange peel-based nanoporous carbon (OPC) is much higher than those of the other types of BNCs. In addition, OPC also shows higher OII uptake capacities from binary dye solutions. The four types of BNCs could be employed as a low-cost alternative for the removal of textile dyes from effluents.

The attenuated Salmonella typhimurium χ4550 strain was used to harbour a reconstructed bicistronic DNA vaccine against porcine rotavirus, which carried the rotavirus nonstructural protein 4 (NSP4) and VP7 genes simultaneously. Using a balanced lethal system, the kanamycin resistance gene of expressing eukaryotic plasmids pVAX1 and pVAXD were replaced by the aspartate β-semialdehyde dehydrogenase (asd) gene. The NSP4 cleavage product (259-525) of rotavirus OSU strain and VP7 full-length genes were amplified by reverse transcription polymerase chain reaction and then inserted into the eukaryotic single-expression plasmid, pVAX1-asd, and the eukaryotic dual-expression plasmid, pVAXD-asd, respectively. The recombinant plasmids pVAX1-asd-NSP4, pVAX1-asd-VP7 and pVAXD-asd-NSP4-VP7 were transformed into the attenuated S. typhimurium χ4550 strain by electrotransformation. An indirect immunofluorescence assay of the expressed COS-7 cell suggested that the recombinant S. typhimurium χ4550 strain was constructed successfully. The recombinant S. typhimurium χ4550 strain was orally administered to BALB/c mice. The group immunised with dual-expression plasmids produced a significantly higher level of serum Immunoglobulin G (IgG) and intestinal Immunoglobulin A (IgA) than the group immunised with single-expression plasmids. These results indicated that eukaryotic bicistronic plasmid DNA vaccines could be successfully constructed to enhance humoural, mucosal and cellular immune response against rotavirus infection.