ABSTRACT Global warming potential (GWP) of rice paddies depends on straw management. This study evaluated methane (CH4) and nitrous oxide (N2O) emissions and soil C stocks to determine GWP and yield-scaled GWP under different strategies and intensities of rice straw management in a subtropical climate. We hypothesized that decreasing soil management intensity and straw incorporation in the soil would reduce GWP. Methane fluxes were substantially higher during the rice growing season than in the off-season, while the opposite was observed for N2O fluxes. The cumulative emissions of CH4 during the growing season among the straw management strategies evaluated ranged from 165.8 to 586.0 kg ha-1. Annual CH4 emissions were lower when soil and straw received some type of management compared to no-tillage. Daily N2O fluxes ranged from -2.8 to 201.7 g ha-1 day-1; cumulative N2O emissions during the off-season ranged from 455.2 to 2816.5 g ha-1. During the off-season, strategies to reduce N2O emissions include post-harvest straw incorporation using a disc harrow, winter straw removal, and ryegrass cropping. Soil organic C stocks ranged from 35.96 to 38.36 Mg ha-1. Straw management using a disc harrow reduced soil organic C stocks, with more adverse effects than straw removal. Soil and rice straw management did not affect rice grain yield, with an average of 10.4 Mg ha-1. Methane emissions were the main component of GWP in all straw management systems. The contribution of N2O emissions to GWP was small and mostly (>85 %) determined by off-season emissions. Yield-scaled GWP ranged from 0.64 to 1.06 Mg CO2eq Mg-1 yield and was lower when soil and straw management systems occurred shortly after the rice harvest. Our results indicate that soil and straw management immediately after rice harvest reduces CH4 emissions, GWP, and yield-scaled GWP.

Abstract The seek for food without the use of agrochemicals has been growing, stimulating organic food production. However, the cultivation of flooded rice in this production system has shown low productivity due to the difficulty in providing nutrients and controlling weeds, compromising the profitability of organic production. Early flood is the principal irrigation management strategy aiming at the control of weeds. Therefore, the objective of this work was to evaluate different phenological stage times of establishment of irrigation in the flooded rice crop in an organic production system. Two experiments were carried out at the Department of Plant Science at the Federal University of Santa Maria, Brazil. The experimental design used was randomized blocks, in a factorial scheme (4x2), with four replications, sown on two dates: on October 10th and December 2nd, 2019. Weed incidence, morphophysiological characteristics, and rice grain yield have been evaluating. Early flooding to the phenological stage is an alternative to control weeds of the genus Echinochloa spp. without causing damage to grain yield in the organic production system.

Resumo O consumo de alimentos sem uso de agroquímicos vem crescendo, estimulando a produção de alimentos orgânicos. Todavia a cultura de arroz irrigado nesse sistema de produção tem apresentado baixa produtividade pela dificuldade no fornecimento de nutrientes e do controle de plantas daninhas, comprometendo a rentabilidade da produção orgânica. A antecipação da irrigação definitiva, constitui-se na principal estratégia de gestão de irrigação visando o controle de infestantes. Neste pressuposto, o objetivo do presente trabalho consistiu em avaliar diferentes épocas do estabelecimento da irrigação, segundo a fenologia do arroz em um sistema orgânico de produção. Foram conduzidos dois ensaios, no Departamento de Fitotecnia na Universidade Federal de Santa Maria, Brasil. O delineamento experimental utilizado foi de blocos ao acaso, em esquema fatorial (4x2), com quatro repetições, semeados em duas épocas: em 10 de outubro e 02 de dezembro de 2019. Foi avaliada a incidência de plantas daninhas, características morfofisiológicas e rendimento de grãos de arroz. A antecipação da irrigação para o estádio fenológico S3 evidenciou ser alternativa no controle de infestantes do gênero Echinocloa spp., não causando prejuízo no rendimento em grão no sistema orgânico de produção.

RESUMO Duas versões contemporâneas do contextualismo radical em filosofia da linguagem, uma defendida por François Recanati e outra por Charles Travis, centram sua crítica à distinção tradicional entre semântica e pragmática na categoria de dito (what is said), tal como descrita por Paul Grice. Ambas as versões se contrapõem à ideia de que o que é dito é determinado plenamente pelo significado convencional da sentença proferida acrescido da fixação do valor de elementos indexicais. Ambas sustentam, a partir desta crítica, que uma enunciação não necessariamente expressa um conteúdo proposicional associado ao significado literal da sentença. Neste artigo, busco mostrar que a partilha desta tese negativa geral esconde divergências importantes. Centrando minha análise na reformulação feita por Recanati da categoria de what is said, busco mostrar como ela se organiza a partir da preservação e radicalização de princípios essenciais da pragmática griceana, bem como da ideia de que elementos subsentencias possuem um conteúdo proposicional mínimo atrelado à sua significação. Em seguida, busco apontar como a negação total da pragmática griceana e deste mínimo proposicional por Travis não apenas revela uma divergência profunda em relação a Recanati, mas gera problemas para a tentativa de reestruturação da noção de what is said.

ABSTRACT François Recanati and Charles Travis have questioned the traditional distinction between semantics and pragmatics by criticizing Grice’s notion of what is said. They are both opposed to the idea that what is said can be read in terms of the conventional meaning of the uttered sentence. Both authors share the thesis according to which what is said does not necessarily put forward a propositional content equivalent to the literal meaning of the uttered sentence. My objective in this paper is to point out important differences between these two positions that are sometimes referred to indistinctively as Radical Contextualism. The paper is centered on the analysis of Recanati’s amendment of the notion of what is said. I first show how his reconstruction of this notion is motivated by the preservation of Grice’s attempt to explain communication in terms of the intention of the speaker. I then proceed to point out how his contextualism admits the idea of a minimal propositional content attached to the meaning of subsentential components. I conclude by arguing that Travis’ rejection of Grice’s whole framework goes against Recanati’s attempt to amend Grice’s notion of what is said.

RESUMO: Apresentando cinco temas centrais no debate sobre a relação educação-trabalho no Brasil, este artigo dá relevo ao fundamento sobre o qual eles estão assentados. Tais temas - a teoria do capital humano; a escola como reprodução; a dualidade estrutural da educação; a perspectiva marxista sobre o papel do trabalho na escola; e a crise do trabalho - ganham evidência a partir da década de 1970 fundados numa nova possibilidade que emerge no discurso educacional: o mundo do trabalho pode, ou deve, impor demandas à escola nacional, alterando sua organização e suas práticas. A tese defendida circunscreve o objeto partilhado por este campo e diz que a possibilidade mencionada é precisamente aquilo a que se dá o nome de educação para o trabalho.

ABSTRACT: Presenting five central themes in the debate about the education-work relationship in Brazil, this paper highlights the foundation over which they are seated. These themes - the human capital theory; school as reproduction; education’s structural duality; the work crisis - gain evidence in the 1970s, founded by a new possibility that emerges from educational discourse: the work world can, or must, impose demands to national school, altering its organization and practices. The thesis defended here circumscribes the object shared by this field and states that the mentioned possibility is precisely what is called education for work.

ABSTRACT Water and oxygen deficiencies in the soybean crop cultivated on lowland soils are an important topic of research. The objective of this study was to investigate changes in water and oxygen supply and soybean yield caused by soil management in lowland soils. A soybean crop was grown under four soil conditions: no-tillage (NT), chiseling (CH), ridge tillage on no-tillage (NTR), and ridge tillage on chiseling (CHR). Soil bulk density, total porosity, macro- and microporosity, air permeability, and saturated hydraulic conductivity were measured at 0.05, 0.15, 0.25, and 0.35 m depths. Soil volumetric water content was monitored at the same depths every 30 min during the soybean cycle. The transpiration coefficient was calculated from volumetric water content to express both water and oxygen deficiency. The groundwater level was monitored throughout the soybean cycle. Plant performance was evaluated by measuring plant population, shoot dry matter, yield, and taproot depth. Soil porosity, air permeability, and saturated hydraulic conductivity were most improved in CH and CHR, and less in NTR. Nonetheless, expected improvement in soil aeration in CH, CHR, and NTR was eliminated when the water table raised to near the soil surface. The transpiration coefficient indicated that CH decreased oxygen deficiency, but caused little water deficit. The CH also provided the highest yield (4,610 kg ha-1), which was not surpassed by the addition of ridge tillage on chiseled soil (CHR) (4,001 kg ha-1). The lowest yields were observed in NT (2,842 kg ha-1), and NTR (3,565 kg ha-1), in which oxygen deficiency was more severe. Lower oxygen deficiency for soybean in chiseled lowland soil is regulated by the water table. As the transpiration coefficient is dependent on all the processes determining soil water dynamics, it is more informative than soil structural properties regarding water and oxygen deficiency in soybean in lowland soil.

O objetivo deste trabalho foi caracterizar o desenvolvimento de cultivares de soja com diferentes grupos de maturação e tipos de crescimento em terras altas e terras baixas no Rio Grande do Sul. Foram conduzidas nove épocas de semeadura em Santa Maria (ambiente de terras altas) e uma época de semeadura em Capão do Leão (ambiente de terras baixas) durante os anos agrícolas 2010/2011, 2011/2012 e 2012/2013, com 11 cultivares de soja. O delineamento experimental em todos os experimentos foi blocos ao acaso, em parcelas subdivididas. Foi quantificada a duração, em ºC dia, do ciclo de desenvolvimento, da fase vegetativa e reprodutiva, e do período entre a data que ocorreu o início do florescimento e o número final de nós para todas as épocas e locais de semeadura. Houve redução da duração da fase reprodutiva, do ciclo total de desenvolvimento (exceto algumas cultivares semeadas em setembro), com o atraso da data de semeadura independentemente do grupo de maturação e tipo de crescimento. As semeaduras de setembro, janeiro e fevereiro promovem uma redução da fase vegetativa na maioria das cultivares quando comparadas com a época recomendada (novembro e dezembro). Cultivares indeterminadas apresentam um maior período de sobreposição das fases vegetativa e reprodutiva do que as cultivares determinadas em todas as épocas e locais de cultivo. O desenvolvimento da soja é similar quando cultivada em terras altas e terras baixas.

The objective of this study was to characterize the development of soybean cultivars with different maturity group, and growth types grown in highlands and in lowlands in Rio Grande do Sul. Field experiments were conducted in Santa Maria (highland) and Capão do Leão (lowland) during the 2010/2011, 2011/2012 and 2012/2013 growing seasons, with 11 treatments (soybean cultivars). Nine sowing dates were conducted in Santa Maria and one in Capão do Leão. The experimental design in all experiments was a randomized block design with split plots. The duration of the development cycle, vegetative and reproductive phases, and the period between the date that was the final number of nodes and early flowering was measured in °C day, for all sites and sowing dates. There was reduction in the duration of the reproductive phase, total development cycle (except some cultivars sown in september), with delay on sowing date regardless of the maturity group and type of growth with delayed sowing. Sowings of September, January and February promote a reduction in vegetative stage in most cultivars when compared with the recommended period (November and December). Cultivars Indeterminate have a longer period of overlap between the vegetative and reproductive phases than certain cultivars in all sites and sowing dates. The soybean development is similar when grown in highlands and lowlands.

PURPOSE:To assess whether late introduction of a specific COX-2 inhibitor (Meloxicam) can treat and/or prevent the progression of tumors in the stomach of rats submitted to duodenogastric reflux. METHODS: Seventy five male Wistar rats, weighing 150 grams, were submitted to the induction of duodenogastric reflux through the pylorus. At 36 weeks of follow-up were established three experimental groups: DGR36 sacrificed immediately, DGR54 and DGR54MLX both sacrificed at 54th week of follow-up . The animals of the latter group were fed with a rat chow premixed with Meloxicam (2.0 mg/ kg feed; 0.3 mg / kg bw / day) and the other two with standard rat chow. The lesions found in the pyloric mucosa and gastrojejunal anastomosis were analyzed macroscopically and histologically. For statistical analysis was adjusted a generalized linear model assuming a binomial distribution with LOGIT link function. RESULTS: No significant differences were found when comparing the incidences of benign tumor lesions (Adenomatous Hyperplasia), p=0.4915, or malignant (Mucinous Adenocarcinoma), p=0.2731, among groups. CONCLUSION: Late introduction of specific COX-2 inhibitor (Meloxicam) did not treat and was not able to prevent the progression of tumoral lesions induced by duodenogastric reflux in the rat stomachs.

PURPOSE: To analyze the clinicopathological features and outcome of patients with pathologically proven superficial squamous cell carcinoma of the esophagus. METHODS: A total of 234 consecutive cases of esophageal carcinoma in a 15-year period were reviewed. RESULTS: Superficial esophageal cancer was found in five patients (2.1%). They were four men and one woman and the mean age was 52.5 years. Smoking and alcohol were the main risk factors. Achalasia due to Chagas disease occurred in one patient and a second primary tumor developed in the larynx in another patient. Four patients underwent esophagectomy and one patient received chemoradiotherapy. The histopathologic diagnosis was of squamous cell carcinoma in all cases. Intramucosal tumor (Tis) was identified in three cases and superficially invasive carcinoma in two cases. Four patients are free of disease with survival times of two, four, six and nine years. The patient who developed laryngeal cancer died six years after esophagectomy. CONCLUSION: Long-term survival in patients with esophageal cancer is related to early diagnosis. Therefore, a less aggressive surgical approach, such as endoscopic resection, may be a good option for these patients, if depth of tumor invasion can be accurately predicted by the new imaging tools.

OBJETIVO: Avaliar as alterações histológicas e biomecânicas do diclofenaco de sódio na mucosa intestinal do rato e a associação com o uso de Imipenem. MÉTODOS: Foram estudados 240 ratos Wistar distribuídos aleatoriamente em quatro grupos experimentais: GI: 60 ratos tratados com injeção IM de soro fisiológico 0,9%; GII: 60 ratos tratados com injeção IM de diclofenaco de sódio na dose de 6mg/kg de peso por 4 dias; GIII: 60 ratos tratados com injeção IM de Imipenem na dose de 30 mg/kg de peso por 4 dias; GIV: 60 ratos tratados com injeção IM de soro fisiológico e diclofenaco de sódio nas doses acima. Em cada grupo os animais foram posteriormente divididos em 4 momentos de 15 animais em cada um para sacrifício, respectivamente, no 4º, 7º, 14º e 21º dias após o início do tratamento. As alterações da cavidade abdominal, assim como as características histológicas e de força de ruptura do intestino delgado foram analisadas em cada momento, em cada grupo. RESULTADOS: Não foram encontradas alterações histológicas e biomecânicas nos animais do Grupo I nesse estudo. Lesões ulceradas na mucosa do intestino delgado foram observadas nos animais tratados com diclofenaco de sódio, assim como diminuição da força de ruptura. As lesões ulceradas encontradas foram prevenidas pelo uso de Imipenem. CONCLUSÃO: O diclofenaco de sódio induz lesões ulceradas na mucosa intestinal do rato que podem ser prevenidas pelo uso de Imipenem.

PURPOSE: To study diclofenac sodium induced histological and mechanical alterations and their prevention with Imipenem in rat intestine. METHODS: Male Wistar rats (n=240) were randomly assigned to four experimental groups: GI: n=60 treated with 0.9% saline IM; GII: n=60 treated with 6mg/kg body weight diclofenac sodium IM for four days; GIII: n=60 treated with 30mg/kg body weight Imipenem IM for four days, and GIV n=60 treated with diclofenac sodium plus Imipenem at the above doses IM for 4 days. Each group was further divided into 4 subgroups of 15 rats each and sacrificed at 4, 7, 14, and 21 days of follow-up, respectively. Abdominal cavity macroscopy and histology, and small bowel breaking strength were analyzed at each sacrifice moment. RESULTS: There were no histological or mechanical alterations in normal control rats throughout the study. Ulcerated lesions in intestinal mucosa were observed and breaking strength decreased in all diclofenac sodium treated rats. Ulcerated lesions in intestinal mucosa were prevented by Imipenem in all rats. CONCLUSION: Diclofenac sodium induced ulcerated lesions in rat intestinal mucosa can be prevented by Imipenem treatment.

Este estudo avaliou, in vitro, por meio de microscopia óptica e morfometria, a limpeza da região apical em canais radiculares com curvatura leve e moderada submetidos ao preparo biomecânico com sistema rotatório, bem como avaliou a quantidade de material extruído para a área apical. Incisivos laterais (n = 32), sendo 16 com ângulo de curvatura igual ou inferior a 10º (GI) e 16 entre 11º e 25º (GII), foram submetidos à instrumentação rotatória com o sistema Hero 642 com diferentes diâmetros cirúrgicos: (A) 30,02 e (B) 45,02. A irrigação, a cada troca de instrumento, foi realizada com água ultra-pura de Milli Q (5 mL), e o material extruído através do forame apical foi coletado. As raízes foram submetidas a análise histológica sob microscopia óptica (40×) e as imagens foram submetidas à análise morfométrica utilizando o software Image Tool. A quantificação do material extruído foi realizada por pesagem, após a evaporação do líquido. A análise de variância não mostrou diferença estatística significante em relação à limpeza da região apical nos diferentes grupos testados (p>0,05). Considerando a quantidade de material extruído, o teste de T6ukey mostrou que os canais com curvatura leve preparados com o diâmetro cirúrgico 45,02 apresentaram os maiores valores, sendo estatisticamente diferente dos demais grupos (p<0,05) que foram semelhantes entre si (p>0,05). Pode-se concluir que a limpeza da região apical não diferiu entre os diferentes grupos, considerando a curvatura radicular e o diâmetro cirúrgico. A quantidade de material extruído foi maior nos canais preparados com curvatura leve que foram preparados com diâmetro cirúrgico 45,02.

Optical microscopy and morphometric analysis were used in this study to evaluate, in vitro, the cleaning of the apical region in root canals with mild or moderate curvatures subjected to biomechanical preparation with a rotary system, as well as to assess the amount of extruded material to the periapical area. Lateral incisors (n = 32), 16 with curvature angles smaller or equal to 10º (GI) and 16 between 11º and 25º angles (GII) were submitted to Hero 642 rotary instrumentation with different surgical diameters: (A) 30.02 and (B) 45.02. Irrigation was performed at each change of instrument with 5 mL of ultrapure Milli-Q water and the extruded material through the apical foramen was collected. Root cross-sections were subjected to histological analysis by optical microscopy (×40) and the images were evaluated morphometrically using the Image Tool software. Quantification of the extruded material was performed by weighing after liquid evaporation. ANOVA showed no statistically significant differences (p>0.05) among the groups with respect to the procedures used to clean the apical region. Considering the amount of extruded material, the Tukey's HSD showed that canals with mild curvature prepared with the 45.02 surgical diameter showed significantly higher values (p<0.05) that those of the other groups, which were similar between themselves (p>0.05). In conclusion, the effect of cleaning the apical region did not differ in the groups, considering root curvature and the surgical diameter of instruments used for apical preparation. The amount of extruded material was greater in canals with mild curvature that were prepared with the 45.02 surgical instrument diameter.

OBJETIVO: Investigar os efeitos do refluxo duodenogástrico e sua interação com o cancerígeno químico N-methil-N'-nitro-nitrosoguanidina (MNNG) no desenvolvimento de lesões no estômago glandular, anastomose gastrojejunal e no estômago escamoso do rato. MÉTODOS: Foram utilizados 80 ratos Wistar divididos em 4 grupos: G1: MNNG + Refluxo, G2: Refluxo, G3: MNNG e G 4: Gastrostomia. O MNNG foi oferecido na água de beber (100mg/ml) por 12 semanas. A seguir foi feita anastomose gastrojejunal na porção glandular do estômago nos grupos G1 e G2, com secção da alça aferente junto ao estômago e sutura de ambos os cotos para permitir o refluxo do conteúdo duodenal para o estômago pelo piloro. Os animais foram sacrificados 18 e 36 semanas após a cirurgia. As lesões identificadas foram submetidas à exame histopatológico. RESULTADOS: O refluxo duodenogástrico levou ao desenvolvimento de lesões proliferativas no estômago glandular e na porção escamosa. No antro, hiperplasia adenomatosa (HA) foi diagnosticada em 20 e 50% dos animais (G2) na 18ª e 36ª semanas, respectivamente. Na anastomose gastrojejunal 85% dos animais (G2) apresentaram HA e 60% apresentaram hiperplasia escamosa no estômago escamoso, na 36ª semana. No grupo MNNG+Refluxo foram identificados na 18ª semana, 30% adenocarcinomas no antro e 45% carcinomas escamosos. A freqüência destas lesões malignas aumentou, respectivamente, para 50% e 65% na 36ª semana. CONCLUSÃO: O refluxo duodenogástrico potencializou o desenvolvimento de lesões proliferativas benignas e malignas no estômago glandular e em sua porção escamosa, no rato.

PURPOSE: To investigate the combined effects of reflux of duodenal contents through the pylorus and treatment with N-methyl-N'-nitro-nitrosoguanidine (MNNG) on the development of lesions in the glandular stomach, at the gastrojejunal anastomosis and in the forestomach of rats. METHODS: Eighty Male Wistar rats were divided into 4 groups: G1: MNNG + Reflux, G2: Reflux, G3: MNNG and G4: Gastrostomy. MNNG was given in the drinking water (100 mg/ml) for 12 weeks and then two groups (G1 and G2) were submitted to a gastrojejunal anastomosis followed by section of the afferent loop and suture of both stumps to allow reflux of duodenal contents through the pylorus. The animals were sacrificed 18 and 36 weeks after surgery. The lesions obtained in the antral mucosa, at the gastrojejunal anastomosis and in the forestomach were analysed histologically. RESULTS: Duodenal reflux induced proliferative lesions at both glandular and squamous mucosa of the stomach. In the antrum, adenomatous hyperplasia (AH) was observed in 20% and 50% of the animals at the 18th and 36th weeks respectively. Aditionally 85% of the animals presented AH at the gastrojejunal anastomosis and 60% developed squamous hyperplasia at the squamous portion of the stomach. MNNG treatment plus duodenal reflux enhanced the development of malignant tumors at both glandular and squamous mucosa, since there were 30% of antral adenocarcinomas and 45% of squamous carcinomas at the 18th week and the frequency of these malignant tumors rose to 50% in the antrum and 65% in the squamous mucosa at the 36th week. CONCLUSION: The reflux of duodenal contents through the pylorus enhanced the development of proliferative lesions, benign and malignant, in the glandular stomach and in the forestomach of rats.

Este estudo busca compreender a dimensão subjetiva de pacientes oncológicos que estudam ou estudaram na escola hospitalar do IOP-GRAACC-UNIFESP. Através de uma metodologia qualitativa baseada na Psicologia sociohistórica, há o objetivo de apreender os sentidos atribuídos pelos sujeitos estudados à escola hospitalar, ao professor hospitalar e à aprendizagem que ocorre nesse contexto. Foi possível observar que a escola hospitalar é marcada pela ausência significativa dos elementos que compõem o sentido acerca da instituição escolar tradicional, ao mesmo tempo em que é vista como um espaço de aprendizagem considerado mais agradável. O professor é carregado de uma carga afetiva bastante positiva, e o saber por ele vinculado ganha aspectos diferenciados. A escola hospitalar deve se afirmar e pautar sua atuação nas suas diferenças, mesmo quando baseadas na ausência, assumindo-se, assim, como espaço escolar específico.

This paper had the objetive of understandig the subjective dimension of the oncological patients who receive an educational provision at the IOP-GRAACC-UNIFESP. Using a qualitative methodology, based on the social-historical psychology, we intended to understand how these students see the hospital school, the teacher who works at the hospital and the kind of apprenticeship that is carried on in this context. We have noticed that the hospital school is marked by a significative absense of the elements that build the way the teenagers see the tradicional school, at the same time that it is seen as a more pleaseant learning environment. The teacher who works at the hospital is loaded with a positive affectivity charge and the subjects he teaches have other functions in this context. The hospital school must act according to its differences, even when they are based on something that is missing, assuming itself as a special scholar enviroment.

Éste estudio busca comprender la dimensión subjetiva de pacientes oncológicos que estudian o estudiaron en la escuela hospitalaria del IOP-GRAACC-UNIFESP. A través de una metodología cualitativa basada en la Psicología sociohistórica, tiene el objetivo de captar los sentidos atribuidos por los sujetos estudiados a la escuela ospitalaria, al profesor hospitalario y al aprendizaje que ocurre en ese contexto. Fue posible observar que la escuela hospitalaria es marcada por la ausencia significativa de los elementos que componen el sentido acerca de la institución escolar tradicional, al mismo tiempo en el que es vista como un espacio de aprendizaje considerado más agradable. El maestro está cargado de una carga afectiva bastante positiva, y el saber por él vinculado gana aspectos diferenciados. La escuela hospitalaria debe afirmarse y pautar su actuación en sus diferencias, incluso cuando son basadas en la ausencia, asumiéndose, así, como espacio escolar específico.

INTRODUÇÃO: Apesar da alta freqüência de infecção por Helicobacter pylori na população, somente uma minoria de indivíduos desenvolve câncer gástrico. É provável que a colonização da mucosa por cepas patogênicas, levando a maior agressão e inflamação da mucosa seja um dos elos da cadeia de eventos da oncogênese gástrica. OBJETIVOS: Investigar a freqüência de cepas patogênicas cagA e vacA do H. pylori em pacientes com câncer gástrico. MATERIAL E MÉTODOS: Foram estudados retrospectivamente 42 pacientes com câncer gástrico. A infecção por H. pylori foi avaliada por exame histológico e pelo PCR para identificação dos genótipos cagA e vacA em amostras de material fixado em formalina e incluído em parafina. RESULTADOS: A análise histológica permitiu a visualização direta do H. pylori em 85,7% dos casos, e o método de PCR para o gene urease C demonstrou a presença de DNA da bactéria em 95% dos casos. O gene cagA foi detectado em amostras de 23 pacientes (54,7%) com câncer gástrico. O alelo s1 do gene vacA foi identificado em amostras de 24 pacientes (57,1%) e o alelo m1, em amostras de 26 pacientes (61,9%). Os alelos s1 e m1 foram identificados simultaneamente em 24 pacientes (57,1%). O alelo s2 foi identificado em amostras de quatro pacientes (9,5%), e o alelo m2, em amostras de três pacientes (7,1%). A freqüência de infecção pelo Helicobacter pylori foi similar em ambos os tipos histológicos de câncer gástrico (intestinal e difuso). CONCLUSÕES: Os resultados confirmam a relevância dos genótipos patogênicos cagA e vacA do H. pylori para lesões orgânicas significativas tais como o câncer gástrico, sugerindo a participação dessa bactéria na cadeia de eventos da oncogênese gástrica.

BACKGROUND: The rates of Helicobacter pylori infection are very high worldwide, but only a minority of infected patients develop gastric carcinoma. This might be related, among several factors, to the colonization of the human stomach by pathogenic Helicobacter pylori strains. OBJECTIVE: To investigate the distribution of cagA and vacA genotypes of Helicobacter pylori in paraffin-embedded gastric samples from patients with gastric cancer. MATERIAL AND METHODS: Paraffin-embedded samples from 42 patients with gastric cancer were histologically examined and evaluated by PCR for H. pylori cagA and vacA (s and m regions) genotypes. RESULTS: Histological analysis allowed direct visualization of H. pylori in 85.7% of cases and PCR for urease C gene detected H. pylori in 95% of cases. The presence of cagA gene was detected in 23 (54.7%) patients with gastric cancer. The s1 allele from vacA gene was found in samples from 24 (57.1%) patients and the m1 allele in 26 (61.9 %). The s1m1 genotype was detected in 24 (57.1%) patients with gastric cancer. The s2 allele was found in samples from four patients (9.5%) and the m2 allele in three (7.1%) patients. The distribution of H. pylori genotypes was similar in both intestinal and diffuse types of gastric carcinoma. CONCLUSION: Our results confirm the relevance of the pathogenic cagA and vacA H. pylori genotypes for significant organic lesions, such as gastric cancer, suggesting a possible role for H. pylori in the pathogenesis of gastric carcinoma.

OBJETIVO: Investigar a incidência de Helicobacter sp em cães oriundos do biotério central da UNESP. MÉTODOS: Utilizamos 109 cães oriundos do Biotério Central da UNESP Campus de Botucatu verificando a presença do Helicobacter sp através do exame de histopatologia corado pelo método Giemsa, pelo teste rápido de urease (TRU) e a prevalência de Helicobacter pylori pelo teste sorológico imunocromatográfico. RESULTADOS: O resultado para a presença de Helicobacter foi de 99% pela histopatologia e pelo TRU e 78,8% de prevalência para a espécie H. pylori pelo teste sorológico. CONCLUSÃO: As formas encontradas pela microscopia de imersão foram contrárias ao resultado do teste sorológico, sendo visibilzado formas compatíveis com a espécie H. heilmannii na maioria das amostras, e uma segunda forma de Helicobacter com características morfológicas distintas do H. pylori encontrado em humanos.

PURPOSE: To investigate the incidence of Helicobacter sp in dogs from the animal colony of UNESP. METHODS: One hundred and nine dogs from the animal colony of UNESP- Botucatu, SP were studied to verify the presence of Helicobacter sp. The methods used were: histopathology stained by Giemsa, rapid urease test (RUT) and the prevalence of Helicobacter pylori was tested by serologic immunocromatography. RESULTS: The presence of Helicobacter was found in 99% of the cases when using the histopathology and RUT methods. The prevalence of H. pylori tested by immunocromatography was of 78.8%. CONCLUSION: The forms found under microscopy oimmersion were contrary to the serologic test, where forms compatible to the H. heilmannii were found in the majority of the samples. A second form of Helicobacter with different morphologic characteristics from the human H. pylori was also found.