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ABSTRACT Cowpeas are of high socioeconomic importance in the North and Northeast regions of Brazil, whereby the search for alternatives that offer increased productivity with financial returns has become an important challenge. Thus, the objective of this study was to estimate and evaluate the economic indicators of cowpea production in 2015 and 2016 when applying drip irrigation to different depths. Irrigation treatments were based on crop evapotranspiration (ETc). The experiment was laid in a randomized complete blocks design with four treatments (0, 25, 50, and 100% ETc) and six replications. For economic analysis, the results were extrapolated to an area of one hectare. Productivity in 2015 was lower than that in 2016; however, in all treatments, it was always higher when plants were grown under adequate water supply. In both years, the total operating costs were higher under irrigation than under rainfed conditions. The 100% ETc treatment resulted in a greater number of cowpea bags (60 kg) produced and a higher gross revenue than the rainfed treatment by 17 bags ha-1, equivalent to 1,020 kg ha-1 (gross revenue US$ 711.48) and 16 bags ha-1, equivalent to 960 kg ha-1 (gross revenue US$ 867.12), in 2015 and 2016, respectively. Water deficit limited cowpea yield in both years but made cultivation economically unfeasible only in 2015.

RESUMO O feijão-caupi apresenta grande importância socioeconômica nas regiões Norte e Nordeste do Brasil, sendo que a busca por alternativas que ofereçam aumento de produtividade com retorno financeiro tem se tornado um importante desafio. Assim, o objetivo deste trabalho foi estimar e avaliar os indicadores econômicos da produção de feijão-caupi nos anos de 2015 e 2016 quando da aplicação de irrigação por gotejamento em diferentes lâminas. Os tratamentos de irrigação foram baseados na evapotranspiração da cultura (ETc). O experimento foi instalado em blocos ao acaso com quatro tratamentos (0, 25, 50 e 100% da ETc) e seis repetições. Para análise econômica, os resultados foram extrapolados para uma área de um hectare. A produtividade em 2015 foi inferior à de 2016; porém, em todos os tratamentos, a produtividade foi sempre maior quando as plantas foram cultivadas sob irrigação adequada. Em ambos os anos, os custos operacionais totais foram maiores sob irrigação do que sob condições de sequeiro. O tratamento 100% ETc resultou em maior número de sacas de feijão-caupi (60 kg) produzidas e receita bruta superior ao tratamento de sequeiro em 17 sacas ha-1, equivalente a 1.020 kg ha-1 (receita bruta US$ 711,48) e 16 sacas ha-1, equivalente a 960 kg ha-1 (receita bruta US$ 867,12), em 2015 e 2016, respectivamente. A deficiência hídrica limitou a produtividade do feijão-caupi nos dois anos, mas inviabilizou economicamente o cultivo apenas em 2015.

https://doi.org/10.1590/1807-1929/agriambi.v27n8p618-624

Seasonal and Circadian Evaluation of the Pectis brevipedunculata Essential Oil and Its Acaricidal Activity against Rhipicephalus microplus (Acari: Ixodidae)
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Camara, Marcos B. P.

; Lima, Aldilene S.

; Jumbo, Luis Oswaldo V.

; Tavares, Caio P.

; Mendonça, Cáritas de Jesus S.

; Monteiro, Odair S.

; Araújo, Sabrina Helena C.

; Oliveira, Eugênio Eduardo de

; Lima Neto, José S.

; Maia, José Guilherme S.

; Costa Junior, Livio M.

; Rocha, Cláudia Q. da

Journal of the Brazilian Chemical Society

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jul 2023, Volume 34 N. 7 Pages 1020 - 1029

Abstract: En | Text: En | PDF: En

Pectis brevipedunculata is native species and widely available in dry and semi-arid ecosystems showing high biotechnological potential. The objective of this study was to evaluate the circadian and seasonal chemical variation of the essential oil (EO) of P. brevipedunculata, as well as its acaricide effect on Rhipicephalus microplus larvae. Aerial parts were collected and submitted to the hydrodistillation process, and the chemical composition was determined by gas chromatography mass spectrometry (GC-MS). For the assays with R. microplus, the larval immersion test was performed. The main constituents were citral (75% of the EO), followed by α-pinene and limonene. In the seasonality analysis, the highest yields were in the months of April (2.08%) and August (2.05%), while in the study of circadian rhythm, the percentage was 2.0% at 6 p.m. in the rainy season, and 1.2%, dry season at 6 p.m. Concerning acaricidal activity (50% lethal concentration (LC50)), the April (1.17 mg mL-1), March (1.28 mg mL-1), June (1.37 mg mL-1), and October (1.27 mg mL-1) oils obtained were the most active and assays performed with circadian rhythm revealed in the rain season (April) at 6 p.m. and dry season (September) at 12 a.m. LC50 values of 1.75 and 1.75 mg mL-1, respectively. Additionally, this EO is selective to non-target organisms, i.e., ladybeetles and lacewing.

https://doi.org/10.21577/0103-5053.20230017

Fitting Structure-Data Files (.SDF) Libraries to Progenesis QI Identification Searches
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Sanches, Pedro H. G.

; Oliveira, Danilo C. de

; Reis, Ivan G. M. dos

; Fernandes, Anna M. A. P.

; Silva, Alex A. R.

; Eberlin, Marcos N.

; Carvalho, Patrícia O.

; Duarte, Gustavo H. B.

; Porcari, Andreia M.

Journal of the Brazilian Chemical Society

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jul 2023, Volume 34 N. 7 Pages 1013 - 1019

Abstract: En | Text: En | PDF: En

Progenesis QI (PQI) is a multiplatform bioinformatics tool that facilitates the identification workflow for metabolomics experiments. PQI uses fragmentation data provided by MassBank of North America (MoNA) libraries, among others, for metabolite annotation. However, PQI does not officially support MoNA libraries and other libraries based on structure-data files (.sdf). This paper describes the development and application of a software named MoNA to Progenesis QI Library Converter, allowing PQI and MoNA by correcting the fragmentation data of the library for Progenesis readability. We evaluated several public experimental datasets, including human plasma, plant extracts, cultured cells, bacteria, rat serum, and rat hippocampus. The results showed that it is mandatory to proceed with file conversion of each library to allow PQI to access fragmentation information from .msp (main spectra profile) files. This step is highly recommended to improve the identification level of the metabolites.

https://doi.org/10.21577/0103-5053.20230016

A prospective validation and comparison of three multivariate models for prediction of difficult intubation in adult patients
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Bicalho, Gustavo P.

; Bessa Jr., Roberto C.

; Cruvinel, Marcos Guilherme C.

; Carneiro, Fabiano S.

; Castilho, Jayme B.

; Castro, Carlos Henrique V.

Brazilian Journal of Anesthesiology

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apr 2023, Volume 73 N. 2 Pages 153 - 158

Abstract: En | Text: En | PDF: En

Abstract Purpose Several bedside clinical tests have been proposed to predict difficult tracheal intubation. Unfortunately, when used alone, these tests show less than ideal prediction performance. Some multivariate tests have been proposed considering that the combination of some criteria could lead to better prediction performance. The goal of our research was to compare three previously described multivariate models in a group of adult patients undergoing general anesthesia. Methods This study included 220 patients scheduled for elective surgery under general anesthesia. A standardized airway evaluation which included modified Mallampati class (MM), thyromental distance (TMD), mouth opening distance (MOD), head and neck movement (HNM), and jaw protrusion capacity was performed before anesthesia. Multivariate models described by El-Ganzouri et al., Naguib et al., and Langeron et al. were calculated using the airway data. After anesthesia induction, an anesthesiologist performed the laryngoscopic classification and tracheal intubation. The sensitivity, specificity, and receiver operating characteristic (ROC) curves of the models were calculated. Results The overall incidence of difficult laryngoscopic view (DLV) was 12.7%. The area under curve (AUC) for the Langeron, Naguib, and El-Ganzouri models were 0.834, 0.805, and 0.752, respectively, (Langeron > El-Ganzouri, p= 0.004; Langeron = Naguib, p= 0.278; Naguib = El-Ganzouri, p= 0.101). The sensitivities were 85.7%, 67.9%, and 35.7% for the Langeron, Naguib, and El-Ganzouri models, respectively. Conclusion The Langeron model had higher overall prediction performance than that of the El-Ganzouri model. Additionally, the Langeron score had higher sensitivity than the Naguib and El-Ganzouri scores, and therefore yielded a lower incidence of false negatives.

https://doi.org/10.1016/j.bjane.2021.07.028

Possible effects of different types of agricultural wastes on food security and mushroom (Pleurotus ostreatus) production
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De La Cruz-Marcos, R. N.

; Areche, F. O.

; Segura, S. O. P.

; López, J. M. M.

; De La Cruz-Calderón, G.

; Solano, M. A. Q.

; Onofre, A. S.

; Camayo-Lapa, B. F.

; Otivo, J. M. M.

; Flores, D. D. C.

; Pairazaman, M. E. E.

; Domínguez, J. A. J.

; Aguilar, S. V.

; Aguirre, L. Á. F.

; Paricanaza-Ticona, D. C.

Brazilian Journal of Biology

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2023, Volume 83 elocation e273829

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract One of the new waters, and environmentally friendly agriculture initiatives in Peru is to encourage the utilization of agricultural waste, because low agricultural output is a threat to food security there. The purpose of this research was to evaluate the effect of harvest residues on the basidiocarp production of the fungus Pleurotus Ostreatus, in Acobamba-Huancavelica. The trial had a completely randomized design, and the treatments included T1, barley stubbles; T2, wheat stubbles; T3, pea stubbles; T4, broad bean stubbles; and T5, quinoa stubbles. The research was quantitative in nature, taking the form of an experiment with an applied, explanatory level of design. The recorded data was tabulated and analyzed with analysis of variance, as well as Tukey's test (α:0.05), for which the statistical software Infostat was used. The results are presented in tables and graphs for a better interpretation. As main results, it was obtained that the time (colonization), diameter (stem, pileus), length (stem) and weight (basidiocarps), present statistical differences between treatments showing significant enhancement in all parameters. Despite a numerical difference, a Tukey average comparison test revealed that there was no statistically significant difference between the averages for the variable time for fungus colonisation, suggesting that the treatment T5 in which quinoa substrate showed the greatest average. Treatment T4 in which broad bean stubbles were used gave the most low-average. In conclusion, increment in all parámeters were noted in all treatment of Pleurotus basidiocarps ostreatus under Acobamba conditions.

Resumo Uma das novas iniciativas de águas e agricultura ecológica no Peru é incentivar a utilização de resíduos agrícolas, porque a baixa produção agrícola é uma ameaça à segurança alimentar no país. O objetivo desta pesquisa foi avaliar o efeito dos resíduos da colheita na produção de basidiocarpos do fungo Pleurotus ostreatus, em Acobamba-Huancavelica. O ensaio teve um delineamento inteiramente casualizado, e os tratamentos incluíram T1, restolho de cevada; T2, soqueiras de trigo; T3, restolho de ervilha; T4, restolho de fava; e T5, restolho de quinoa. A pesquisa foi de natureza quantitativa, assumindo a forma de experimento com um nível de design aplicado e explicativo. Os dados registrados foram tabulados e analisados com análise de variância, bem como teste de Tukey (α:0,05), para o qual foi utilizado o software estatístico Infostat. Os resultados são apresentados em tabelas e gráficos para melhor interpretação. Como principais resultados, obteve-se que o tempo (colonização), diâmetro (caule, píleo), comprimento (caule) e peso (basidiocarpos) apresentam diferenças estatísticas entre os tratamentos, mostrando melhora significativa em todos os parâmetros. Apesar da diferença numérica, o teste de comparação de médias de Tukey revelou que não houve diferença estatisticamente significativa entre as médias para a variável tempo de colonização do fungo, sugerindo que o tratamento T5 em que usou o substrato quinoa, apresentou a maior média. O tratamento T4, no qual foram usadas restolhos de fava, apresentou a média mais baixa. Em conclusão, incrementos em todos os parâmetros foram observados em todos os tratamentos de Pleurotus basidiocarps ostreatus nas condições de Acobamba.

https://doi.org/10.1590/1519-6984.273829

Long-term Ecological Research: Chasing fashions or being prepared for fashion changes?
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BERGALLO, HELENA G.

; ROSA, CLARISSA

; OCHOA, ANA C.

; MANZATTO, ANGELO GILBERTO

; GUIMARAES, ARETHA F.

; BANHOS, AUREO

; CASTILHO, CAROLINA V.

; BARROS, CLAUDIA F.

; NORRIS, DARREN

; DRUCKER, DEBORA P.

; RODRIGUES, DOMINGOS J.

; BACCARO, FABRICIO B.

; LOURENÇO, IGOR H.

; ZUANON, JANSEN

; STEGMANN, LIS F.

; ANJOS, MARCELO R.

; SILVEIRA, MARCOS

; ARAÚJO, PATRICIA S.G.

; BOBROWIEC, PAULO E.D.

; FADINI, RODRIGO

; NECKEL-OLIVEIRA, SELVINO

; EMILIO, THAISE

; SANTORELLI JUNIOR, SERGIO

; MAGNUSSON, WILLIAM E.

Anais da Academia Brasileira de Ciências

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2023, Volume 95 N. 3 elocation e20230051

Abstract: En | Text: En | PDF: En

Abstract Long-term-ecological-research (LTER) faces many challenges, including the difficulty of obtaining long-term funding, changes in research questions and sampling designs, keeping researchers collecting standardized data for many years, impediments to interactions with local people, and the difficulty of integrating the needs of local decision makers with “big science”. These issues result in a lack of universally accepted guidelines as to how research should be done and integrated among LTER sites. Here we discuss how the RAPELD (standardized field infrastructure system), can help deal with these issues as a complementary technique in LTER studies, allowing comparisons across landscapes and ecosystems and reducing sampling costs. RAPELD uses local surveys to understand broad spatial and temporal patterns while enhancing decision-making and training of researchers, local indigenous groups and traditional communities. Sampling of ecological data can be carried out by different researchers through standardized protocols, resulting in spatial data that can be used to answer temporal questions, and allow new questions to be investigated. Results can also be integrated into existing biodiversity networks. Integrated systems are the most efficient way to save resources, maximize results, and accumulate information that can be used in the face of the unknown unknowns upon which our future depends.

https://doi.org/10.1590/0001-3765202320230051

High rDNA polymorphisms in Astyanax lacustris (Characiformes: Characidae): new insights about the cryptic diversity in A. bimaculatus species complex with emphasis on the Paraná River basin
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Tonello, Sandro

; Blanco, Daniel R.

; Cerqueira, Fiorindo J.

; Lira, Natália L.

; Traldi, Josiane B.

; Pavanelli, Carla S.

; Margarido, Vladimir P.

; Gavazzoni, Mariane

; Pupo, Marcos V.

; Lui, Roberto L.

Neotropical Ichthyology

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2022, Volume 20 N. 2 elocation e210147

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Este trabalho teve como objetivo identificar espécies do complexo Astyanax bimaculatus de quatro afluentes do reservatório de Itaipu (bacia do Rio Paraná) por métodos citogenéticos e moleculares (COI), investigando a possibilidade de ocorrência de diversidade críptica em parte desta bacia. As quatro populações apresentaram apenas uma fórmula cariotípica e AgNORs simples. A FISH com rDNA 18S apresentou alto grau de variação e as sondas de rDNA 5S evidenciaram sítios simples na maioria dos exemplares, embora sítios múltiplos tenham sido evidenciados em dois espécimes. As variações dos cistrons 5S e 18S geraram 13 citótipos. Os dados moleculares não revelaram diversidade críptica nas populações, entretanto, seu agrupamento com 82 sequências de outros trechos da mesma bacia formou três haplogrupos (distâncias de 3,12% e 8,82%) e gerou 33 haplótipos. O DNA Barcode sugere que as variações citogenéticas representam um alto grau de polimorfismo e identificou os espécimes analisados como Astyanax lacustris, confirmando a identificação por caracteres morfológicos. Nossos dados sugerem que a diversidade críptica do grupo nos afluentes da bacia do Rio Paraná é diferente do proposto pelas sinonimizações de A. altiparanae e A. asuncionensis para A. lacustris, reforçando a importância da integração de métodos citogenéticos e moleculares para a taxonomia.

Abstract This study aimed to identify species of Astyanax bimaculatus group from four Itaipu Reservoir tributaries (Paraná River Basin) by cytogenetics and molecular markers (COI) to investigate the possible occurrence of cryptic diversity in part of this basin. The four populations showed only one karyotype formula and simple AgNORs. FISH with 18S rDNA probe showed a high variation, and 5S rDNA probes evidenced simple sites in most of the specimens, although multiple sites are present in two specimens. The variations of 5S and 18S cistrons generated 13 cytotypes. The molecular data did not reveal cryptic diversity in the populations; however, its grouping with 82 sequences from other stretches of the Paraná River Basin originated three haplogroups (distances of 3.12% and 8.82%) and 33 haplotypes were identified. DNA Barcode suggests that cytogenetic variations represent a high polymorphism degree, and it identified the analyzed specimens as Astyanax lacustris, which confirms the morphological identification. Our data suggest that the cryptic diversity of this group in the tributaries of the Paraná River Basin is different than the proposed by the synonymizations of A. altiparanae and A. asuncionensis to A. lacustris. This study reinforces the importance of integrative cytogenetics and molecular methods for taxonomy.

https://doi.org/10.1590/1982-0224-2021-0147

Hydro-physical properties of soil and pasture vegetation coverage under animal trampling
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Batista, Pedro H. D.

; Almeida, Gledson L. P. de

; Silva, Jhon L. B. da

; Lins, Frederico A. C.

; Silva, Marcos V. da

; Cordeiro Junior, Jose J. F.

Revista Brasileira de Engenharia Agrícola e Ambiental

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dec 2020, Volume 24 N. 12 Pages 854 - 860

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A compactação do solo e a degradação das pastagens muitas vezes são provenientes da pressão exercida pelo casco dos animais. Nesse sentido, objetivou-se verificar possíveis modificações no solo e mudanças na cobertura vegetal da pastagem devido ao pisoteio animal. Assim, um estudo foi realizado em uma fazenda na região do Agreste de Pernambuco, Brasil. Em uma área de 40 × 40 m manejada sob pastejo contínuo, com uma taxa de lotação de 12 UA ha-1; as amostras de solo foram coletadas antes e após o período de pastejo, com 36 pontos distribuídos regularmente. Em cada ponto, determinou-se a densidade, porosidade total, umidade e resistência à penetração do solo na profundidade de 0-0,10 m, bem como a condutividade hidráulica e a matéria orgânica na superfície do solo. Antes e após o pastejo, imagens do satélite Sentinel-2A também foram obtidas para observar o padrão de resposta da vegetação da pastagem ao longo do tempo por meio do Índice de Vegetação por Diferença Normalizada (NDVI) e Cobertura Vegetal de Pastagem (CVP). Os atributos físico-hídricos do solo foram submetidos a análise fatorial. Os mapas de NDVI e CVP foram avaliados quanto à variabilidade espacial. Após o período de pastejo, houve alteração nos atributos do solo em estudo devido ao pisoteio dos animais. O sensoriamento remoto (NDVI e CVP) destacou padrões de variação, como a redução da pastagem e possíveis áreas de preferencias dos animais.

ABSTRACT Soil compaction and pasture degradation are often due to the pressure exerted by the animals’ hooves. In this sense, this study aimed to verify possible changes in the soil and changes in the pasture vegetation coverage due to animal trampling. Thus, a study was carried out on a farm in the ‘Agreste’ region of Pernambuco, Brazil. Soil samples were collected before and after the grazing period, with 36 points regularly distributed in an area of 40 × 40 m managed under continuous grazing, with a stocking rate of 12 AU ha-1. At each point, density, total porosity, humidity, and penetration resistance of the soil at a depth of 0-0.10 m, as well as hydraulic conductivity and organic matter in the soil surface, were determined. Before and after grazing, images from the Sentinel-2A satellite were also obtained to observe the response pattern of pasture vegetation over time using the Normalized Difference Vegetation Index (NDVI) and Pasture Vegetation Coverage (PVC). The physical-hydric attributes of the soil were subjected to factor analysis. NDVI and PVC maps were evaluated for spatial variability. After the grazing period, there was a change in the attributes of the soil under study due to the animal trampling. Remote sensing (NDVI and PVC) highlighted patterns of variation, such as the reduction of pasture and possible areas of preference for the animals.

https://doi.org/10.1590/1807-1929/agriambi.v24n12p854-860 589 downloads

Electroanalytical Method for Determination of Trace Metals in Struvite Using Electrochemically Treated Screen-Printed Gold Electrodes
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Vasconcellos, Maria L. S.

; Rocha, Diego P.

; Castro, Sílvia V. F.

; Silva, Luiz R.G. e

; Muñoz, Rodrigo A. A.

; Freitas, Marcos B. J. G.

; Ferreira, Rafael Q.

Journal of the Brazilian Chemical Society

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sep 2020, Volume 31 N. 9 Pages 1873 - 1882

Abstract: En | Text: En | PDF: En

We present an electroanalytical method using square-wave anodic stripping voltammetry on electrochemically-treated screen-printed gold electrodes to the simultaneous determination of lead(II), copper(II) and mercury(II) in struvite (natural fertilizer produced from urine). Electrochemical treatment via cyclic voltammetry of the working electrodes increased surface rugosity that improved the electroanalytical responses for the three metals. Under optimized conditions and using a deposition time of 90 s, the proposed method presented the following limit of detection values: 1.3 µg L-1 for lead(II), 2.0 µg L-1 for copper(II) and 0.9 µg L-1 for mercury(II). Struvite samples were easily dissolved in supporting electrolyte solution (0.05 mol L-1 HCl). Satisfactory recovery values between 91 and 111% were obtained for the analysis of spiked samples. Hence, we can infer that the proposed electroanalytical method is an effective alternative for the determination of trace metals, since it provides precise and accurate direct analyses, with rapid responses without interference from sample matrix and enabling in field analysis.

https://doi.org/10.21577/0103-5053.20200081 404 downloads

10.

Directional Coupler Based on a Photonic Quasi-Crystal Fiber with Extended Cores
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Silva, J. P. da

; Santos, Einstein G. dos

; Segundo, Marcos T. B.

Journal of Microwaves, Optoelectronics and Electromagnetic Applications

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jun 2018, Volume 17 N. 2 Pages 201 - 207

Abstract: En | Text: En | PDF: En

Abstract In this paper, an optical coupler composed by a photonic quasi-crystal fiber (PQCF) with two cores is proposed. The structure is obtained by immersion of a quasi-periodic arrangement of air holes replicated twelve-fold in a conventional fiber optical constituted of pure silica. The cores are generated from defects caused due to absence of two air holes on each identical core and horizontally equidistant from the center of PQCF. The chromatic dispersion in each fiber core is ultra-flat around 38.12 ps.nm-1.km-1 and to verify the power transfer, the signal was launched on the left core and transferred to the right core in a propagation length of approximately 3.78 cm.

https://doi.org/10.1590/2179-10742018v17i21137 599 downloads

11.

Self-report of psychological symptoms in hypoparathyroidism patients on conventional therapy
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Arneiro, Amanda J.

; Duarte, Bianca C. C.

; Kulchetscki, Rodrigo M.

; Cury, Victor B. S.

; Lopes, Maicon P.

; Kliemann, Breno S.

; Bini, Ileana B.

; Assad, Marcos

; Biagini, Gleyne L. K.

; Borba, Victoria Z. C.

; Moreira, Carolina A.

Archives of Endocrinology and Metabolism

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may 2018, Volume 62 N. 3 Pages 319 - 324

Abstract: En | Text: En | PDF: En

ABSTRACT Objective: Hypoparathyroidism is characterized by parathyroid hormone deficiency and hypocalcemia. It has been demonstrated that these patients may also present psychiatric symptoms and decrease of quality of life. The aims of this study were to evaluate the presence of psychopathological symptoms in a cohort of patients with hypoparathyroidism and compare to a control group. Subjects and methods: Patients were submitted to a cross-sectional Symptom Checklist-90-R (SCL-90-R) questionnaire that evaluates psychopathological symptoms by means of the Global Severity Index (GSI), Positive Symptoms Total (PST) and Positive Symptom Distress Index (PSDI). A score based in the positive symptoms was calculated (T-score). The test group was composed of patients with hypoparathyroidism, and control by thyroidectomized patients without hypoparathyroidism. A correlation between the presence of psychological symptoms and clinical features was analyzed. Results: The study included 57 patients with a mean age of 51.1 ± 16.4 years; 20 as a control and 37, test group. There were no differences between groups regarding gender, mean age and age at diagnose. Hypoparathyroidism patients presented higher GSI index than the control group (p = 0.038). Mean T-score of the test group was as elevated as 58.2 ± 5.3 (reference range < 55). No correlation of the number of psychological symptoms to clinical and laboratorial parameters was observed. Conclusion: Patients with hypoparathyroidism attending our outpatient clinics presented an increase in the number of self-report of psychological symptoms when compared with a control group. However, no correlation with hypocalcemia and clinical parameters was observed. Future studies are necessary to evaluated if the absence of PTH play a role on it.

https://doi.org/10.20945/2359-3997000000041 1231 downloads

12.

Design, Synthesis, Biological Evaluation and Molecular Modeling Studies of Novel Eugenol Esters as Leishmanicidal Agents
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Coelho, Camila M.

; Santos, Thiago dos

; Freitas, Poliany G.

; Nunes, Juliana B.

; Marques, Marcos J.

; Padovani, Camila G. D.

; Júdice, Wagner A. S.

; Camps, Ihosvany

; Silveira, Nelson J. F. da

; Carvalho, Diogo T.

; Veloso, Marcia P.

Journal of the Brazilian Chemical Society

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apr 2018, Volume 29 N. 4 Pages 715 - 728

Abstract: En | Text: En | PDF: En

Leishmaniasis is a neglected pathology with a high incidence worldwide, and is a governmental health issue due to the increased morbidity and mortality associated with the disease and a scarce therapeutic arsenal. Cysteine proteases have been investigated as targets for new drugs because they are essential in the infectivity of the parasite during its interaction with the host and in its nutrition. This study aimed to identify compounds with leishmanicidal activity, by synthesis of compounds, in vitro evaluation of their biological activity and using molecular modeling and bioinformatics tools. The study of biological activity demonstrated that one compound showed inhibitory activity against the enzyme rCPB 2.8 at a concentration of 100 µmol L-1. Activation of the enzyme rCPB 2.8 by other 4 compounds was also verified, which may be related to the interaction of these compounds with an allosteric site on the enzyme.

https://doi.org/10.21577/0103-5053.20170192 1071 downloads

13.

Phenotype-driven strategies for screening Candida parapsilosis complex for molecular identification
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Cordeiro, Rossana A.

; Sales, Jamille A.

; Ponte, Yago B. de

; Mendes, Patrícia B.L.

; Serpa, Rosana

; Evangelista, Antônio J.

; Alencar, Lucas P. de

; Pereira-Neto, Waldemiro de A.

; Brilhante, Raimunda S.N.

; Sidrim, José J.C.

; Castelo-Branco, Débora S.C.M.

; Rocha, Marcos F.G.

Brazilian Journal of Microbiology

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2018, Volume 49 Suppl. 1 Pages 193 - 198

Abstract: En | Text: En | PDF: En

Abstract In this study, phenotypic methods presented >80% agreement with the molecular identification of 59 Candida parapsilosis complex. Growth at 15% NaCl or pH 7.0 significantly reduced cfu-counts of Candida orthopsilosis, suggesting these conditions may support the development of phenotypic methods for the differentiation of the cryptic species of C. parapsilosis complex.

https://doi.org/10.1016/j.bjm.2017.11.004 729 downloads

14.

Quality of life in patients treated with methadone: The WHOQOL-BREF, psychometric study and application results
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Pedrero-Pérez, Eduardo J.

; Barreda-Marina, M.A.

; Bartolomé-Gil, C.

; Bosque-Coro, S.

; Callejo-Escobar, J.

; Ema-López, I.

; Dominguez-Aranda, M.A.

; Ferrero-Herreros, Y.E.

; Galera-García, Ó.

; Garrido-Ureña, B.

; Gil de Bernabe-Lopez, M.J.

; González-Galnares, I.R.

; Gutiérrez-Cáceres, S.

; Heras-Dolader, S.

; Hernández-Tejada, C.

; López-Jiménez, M.C.

; López-Zurita, C.

; Martin-Carmona, G.

; Notario-Poves, P.

; Olmos-Espinosa, R.

; Pacheco-Otoya, G.

; Pérez-Carrasco, E.

; Pérez-López, G.

; Puerta-García, C.

; Rojo-Mota, G.

; Sáez-Maldonado, A.

; Salgado-Marcos, N.

; San Juan-Sanz, P.

; Sújar-Plaza, M.I.

; Borrallo-Berjón, M.J.

; Boticario-Villarroel, M.V.

; Bueno-Pozo, R.

; Iglesias-Jiménez, M.F.

; Mateos-Ayucar, M.P.

Anales de Psicología

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2018, Volume 34 N. 2 Pages 251 - 257

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract The most commonly instrument used to measure quality of life in patients with addictive behaviors is the WHOQOL-BREF, developed by the World Health Organization. No studies have been found to explore the psychometric properties in Spanish clinical samples. This paper aims to study their reliability as well as the construct validity in a representative sample of patients and comparing the results to the data available for the general population in Spain. A sample was recruited comprised of 523 subjects who were undergoing treatment with methadone in Madrid and Extremadura. A confirmatory factor analysis was completed to test the theoretically proposed structure and then an optimized-parallel analysis was done to establish the most adequate number of components. The result offers solid values for internal consistency both as concerns the items and the scales. The theoretical tetra-dimensional structure is confirmed in the sample although serious arguments are also found for considering its one-dimensionality. The structural relationship between the four domains was studied. The WHOQOL-BREF proves to be a reliable and valid test for use on patients treated with methadone, providing a multi-dimensional measure of perceived quality of life that includes social and environmental factors of great importance in treating addiction problems.

Resumen Objetivo. De entre los muchos instrumentos propuestos para medir la calidad de vida de los pacientes con adicción a opiáceos tratados con metadona el WHOQOL-BREF, propuesto por la World Health Organization, es el que más se utiliza en la actualidad. Este trabajo pretende estudiar la fiabilidad y la validez de constructo de la prueba, aplicada a una muestra amplia y representativa de pacientes, y comparar los resultados con los datos disponibles para población general en España. Se reclutó una muestra de 523 sujetos que se encontraban en tratamiento con metadona, en Madrid y Extremadura. Se efectuó un análisis factorial confirmatorio para probar la estructura teóricamente propuesta y, seguidamente, un análisis paralelo optimizado para conocer el número más adecuado de componentes de la prueba. Los resultados muestran que la prueba presenta sólidos valores de consistencia interna, tanto a nivel del ítem como de las escalas. La estructura tetradimensional teórica se confirma en la muestra con adecuados indicadores de ajuste, aunque también se obtienen serios argumentos para considerar su unidimensionalidad. Se estudió la relación estructural entre los cuatro dominios. El WHOQOL-BREF se muestra como una prueba fiable y válida para su uso en pacientes tratados con metadona, proporcionando una medida multidimensional de la calidad de vida percibida, que incluye factores sociales y ambientales ausentes en otros instrumentos, que son de enorme importancia en el tratamiento de los problemas adictivos.

https://doi.org/10.6018/analesps.34.2.282971 416 downloads

15.

Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts
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PINTO, NÍCOLAS C.C.

; SILVA, JUCÉLIA B.

; MENEGATI, LAURA M.

; GUEDES, MARIA CLARA M.R.

; MARQUES, LUCAS B.

; SILVA, THIAGO P. DA

; MELO, ROSSANA C.N. DE

; SOUZA-FAGUNDES, ELAINE M. DE

; SALVADOR, MARCOS J.

; SCIO, ELITA

; FABRI, RODRIGO L.

Anais da Academia Brasileira de Ciências

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2017, Volume 89 N. 3 Pages 2053 - 2073

Abstract: En | Text: En | PDF: En

ABSTRACT This study aimed to further investigate the cytotoxicity against tumor cell lines and several bacterial strains of Annona squamosa and its mode of action. Methanol extracts of A. squamosa leaves (ASL) and seeds (ASS) were used. ASL showed significant antibacterial activity against S. aureus, K. pneumoniae and E. faecalis with MIC values of 78, 78 and 39 µg/mL respectively. Moreover, ASL exhibited significant biofilm disruption, rapid time dependent kinetics of bacterial killing, increased membrane permeability and significantly reduced the cell numbers and viability. Regarding the cytotoxicity against tumor cell lines, ASS was more active against Jurkat and MCF-7 cells, with CI50 1.1 and 2.1 µg/mL, respectively. ASL showed promising activity against Jurkat and HL60, with CI50 4.2 and 6.4 µg/mL, respectively. Both extracts showed lower activity against VERO cells and reduced the clonogenic survival at higher concentrations (IC90) to MCF-7 and HCT-116 lineages. The alkaloids anonaine, asimilobine, corypalmine, liriodenine nornuciferine and reticuline were identified in extracts by UPLC-ESI-MS/MS analysis. This study reinforced that A. squamosa presents a remarkable phytomedicinal potential and revealed that its antimicrobial mechanism of action is related to bacterial membrane destabilization.

https://doi.org/10.1590/0001-3765201720150702 2182 downloads

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