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Resumo O fungo micotoxigênico Fusarium verticillioides é o principal patógeno do milho e causa doenças do colmo e da podridão da espiga com perdas econômicas significativas. Além disso, o uso excessivo de fungicidas no controle de F. verticillioides constitui uma ameaça ao meio ambiente e à saúde humana. Assim, alternativas sustentáveis, como o controle biológico, são necessárias para minimizar os riscos associados ao método atual. Este estudo isolou e caracterizou bactérias e fungos que colonizam o estigma do milho para avaliar novos agentes de biocontrole em potencial. As amostras foram coletadas em campos de milho nas localidades brasileiras de Sete Lagoas-MG, Sidrolândia-MS, Sertaneja-PR e Goiânia-GO. Cento e sessenta e sete microrganismos foram isolados, 46% endofíticos e 54% epifíticos. O teste de antagonismo empregando a técnica de disco de difusão em meio sólido, mostrou que 83% dos isolados apresentaram atividade antagonista contra F. verticillioides. Em seguida, 42 isolados mais eficientes foram identificados a partir do sequenciamento parcial do gene 16S rRNA bacteriano e da região ITS de fungos. Os isolados bacterianos pertencem ao gênero Bacillus (57,1%), Burkholderia (23,8%), Achromobacter (7,1%), Pseudomonas (2,4%) e Serratia (2,4%), enquanto os fungos são Penicillium (2,4%), Candida (2.4), e Aspergillus (2,4%). Os resultados mostraram que microrganismos do estigma do milho podem representar novos agentes promissores para o controle de F. verticillioides.

Abstract The mycotoxigenic fungus Fusarium verticillioides is the primary maize pathogen and causes the maize stalk and ear rot diseases with significant economic losses. Furthermore, the excessive use of fungicides to control F. verticillioides constitutes threats to the environment and human health. Thus, sustainable alternatives such as biological control are needed to minimize the hazards associated with the current method. Although much is known about the vulnerability of the maize silks as a gateway for several fungal pathogens invading the developing grains, studies on the chemical properties of silk extracts and their resident microbiota are scarce. This study isolated and characterized bacteria and fungi that colonize the maize stigma to assess new potential biocontrol agents. The samples were collected from maize fields in the Brazilian localities of Sete Lagoas-MG, Sidrolândia-MS, Sertaneja-PR, and Goiânia-GO. One hundred sixty-seven microorganisms were isolated, 46% endophytic and 54% epiphytic. First, the antagonist activity was evaluated by the agar disc diffusion method performed in triplicate, and 83% of the isolates showed antagonist activity against F. verticillioides. Then, the 42 most efficient isolates were identified based on the partial sequencing of the bacterial 16S rRNA gene and fungi ITS region. The bacteria belong to the genera Bacillus (57.1%), Burkholderia (23.8%), Achromobacter (7.1%), Pseudomonas (2.4%), and Serratia (2.4%), while the fungi are Penicillium (2.4%), Candida (2.4), and Aspergillus (2.4%). The results showed that microorganisms from maize stigma might represent new promising agents for F. verticillioides control.

https://doi.org/10.1590/1519-6984.262567

Antidiabetic and hypolipidemic potential of Campomanesia xanthocarpa seed extract obtained by supercritical CO2
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Regginato, A.

; Cunico, L.

; Bertoncello, K.T.

; Schindler, M. S. Z.

; Chitolina, R.

; Marins, K.

; Zanatta, A. P.

; Calisto, J. F.

; Oliveira, J. V.

; Magro, J. D.

; Zanatta, L.

Brazilian Journal of Biology

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sep 2021, Volume 81 N. 3 Pages 621 - 631

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Campomanesia xanthocarpa, planta pertencente à família Mirtaceae, é popularmente conhecida como gabiroba. Folhas da gabiroba são popularmente usadas para tratar de doenças inflamatórias, renais, digestivas entre outras. Além disso, estudos têm mostrado um efeito redutor dos níveis de colesterol. O objetivo deste estudo foi avaliar os efeitos anti-hiperglicêmico e hipolipidêmico do extrato de sementes de Campomanesia xanthocarpa em ratos hiperglicêmicos. Os resultados mostraram que 400 mg/kg do extrato da semente foi capaz de reduzir os níveis de glicose sanguínea e aumentar o conteúdo de glicogênio hepático e muscular, bem como inibir a atividade da maltase e sacarase. Na dose de 200 mg/kg e 800 mg/kg, a atividade das enzimas também foi reduzida. No perfil lipídico, 400 mg/kg produziu uma redução nos níveis séricos de colesterol total e LDL e com 200 mg/kg houve um aumento nos níveis de colesterol HDL. O extrato não apresentou efeitos tóxicos hepáticos e renais nas doses testadas. Os resultados sugerem que o tratamento com o extrato de Campomanesia xanthocarpa é eficaz na redução da glicemia, de colesterol total e LDL com potencial para tratamento adjuvante do diabetes e hipercolesterolemia, no entanto estudos farmacológicos e toxicológicos adicionais são necessários.

Abstract Campomanesia xanthocarpa, a plant belonging to the Myrtaceae family, is popularly known as gabiroba. Leaves of gabiroba has been popularly used to treat various diseases, including inflammatory, renal, and digestive, among others. Additionally, studies have shown an effect to reduce blood cholesterol levels. The aim of this study was to evaluate the antihyperglycemic and hypolipidemic effects of Campomanesia xanthocarpa seed extract in hyperglycemic rats. The results showed that 400 mg/kg of seed extract was able to decrease blood glucose levels and to increase the muscular and hepatic glycogen content as well as to inhibit the sucrase and maltase activity. At doses of 200 mg/kg and 800 mg/kg, the activity of these enzymes was also reduced. In the lipid profile 400 mg/kg produced a decrease in total and LDL cholesterol serum levels; and with 200 mg/kg there was an increase in HDL cholesterol levels. The extract did not present hepatic and renal toxic effects at the different doses tested. The results suggest that the treatment with Campomanesia xanthocarpa seeds extract is useful in reducing glycemia, total cholesterol and LDL levels with potential adjuvant therapeutic in the treatment of diabetes and hypercholesterolemia, however, additional pharmacological and toxicological studies are still required.

https://doi.org/10.1590/1519-6984.227388 2010 downloads

Baryon Acoustic Oscillations from Integrated Neutral Gas Observations: an instrument to observe the 21cm hydrogen line in the redshift range 0.13 < z < 0.45 – status update
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WUENSCHE, CARLOS A.

; ABDALLA, ELCIO

; ABDALLA, FILIPE

; BAROSI, LUCIANO

; WANG, BIN

; AN, RUI

; BARRETOS, JOÃO A. M.

; BATTYE, RICHARD

; BRITO, FRANCISCO A.

; BROWNE, IAN

; CORREIA, DANIEL

; COSTA, ANDRÉ A.

; DELABROUILLE, JACQUES

; DICKINSON, CLIVE

; FENG, CHANG

; FERREIRA, ELISA G. M.

; FORNAZIER, KARIN

; DE GASPERIS, GIANCARLO

; GUTIERREZ, PRISCILA

; HARPER, STUART

; LANDIM, RICARDO G.

; LICCARDO, VINCENZO

; MA, YIN-ZHE

; MACHADO, TELMO

; MAFFEI, BRUNO

; MARINS, ALESSANDRO

; MENDES, MILENA M. M.

; MERICIA, EDUARDO

; MONSTEIN, CHRISTIAN

; MOTTA, PABLO

; NOVAES, CAMILA

; OTOBONE, CARLOS H.

; PEEL, MICHAEL

; QUEIROZ, AMILCAR R.

; RADCLIFFE, CHRISTOPHER

; REMAZEILLES, MATHIEU

; RIBEIRO, RAFAEL M. G.

; SANG, YU

; SANTOS, JULIANA F. R.

; SANTOS, LARISSA

; SANTOS, MARCELO. V.

; SHAN, CHENXI

; SILVA, GUSTAVO B.

; VIEIRA, FREDERICO

; VIEIRA, JORDANY

; VILLELA, THYRSO

; XIAO, LINFENG

; YANG, WEIQIANG

; ZHANG, JIAJUN

; ZHANG, XUE

; ZHU, ZENGHAO

Anais da Academia Brasileira de Ciências

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2021, Volume 93 elocation e20201096

Abstract: En | Text: En | PDF: En

Abstract BINGO (BAO from Integrated Neutral Gas Observations) is a unique radio telescope designed to map the intensity of neutral hydrogen distribution at cosmological distances, making the first detection of Baryon Acoustic Oscillations (BAO) in the frequency band 980 MHz - 1260 MHz, corresponding to a redshift range 0.127 < z < 0.449. BAO is one of the most powerful probes of cosmological parameters and BINGO was designed to detect the BAO signal to a level that makes it possible to put new constraints on the equation of state of dark energy. The telescope will be built in Paraíba, Brazil and consists of two \thicksim 40m mirrors, a feedhorn array of 50 horns, and no moving parts, working as a drift-scan instrument. It will cover a 15 ^{\circ} ∘ declination strip centered at \sim \delta ∼ δ =-15 ^{\circ} ∘, mapping \sim ∼ 5400 square degrees in the sky. The BINGO consortium is led by University of São Paulo with co-leadership at National Institute for Space Research and Campina Grande Federal University (Brazil). Telescope subsystems have already been fabricated and tested, and the dish and structure fabrication are expected to start in late 2020, as well as the road and terrain preparation.

https://doi.org/10.1590/0001-3765202120201096 413 downloads

Systemic Lithium Chloride Administration Improves Tooth Extraction Wound Healing in Estrogen-Deficient Rats
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Duarte, Poliana M.

; Miranda, Tamires S.

; Marins, Letícia M.

; Perez, Erick G.

; Copes, Liliana G.

; Tonietto, Cristine B.

; Montalli, Victor A. M.

; Malta, Fernando S.

; Napimoga, Marcelo H.

Brazilian Dental Journal

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nov 2020, Volume 31 N. 6 Pages 640 - 649

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O objetivo deste estudo foi avaliar os efeitos do Cloreto de Lítio (ClLi) na cicatrização de alvéolos de ratas deficientes em estrogênio. Setenta e duas ratas foram alocadas em um dos seguintes grupos: Controle, Ovariectomia e Cloreto de Lítio (150mg/kg/ oralmente a cada 2 dias) + ovarectomia. Os animais receberam ClLi ou água a partir do 14º dia pós-ovariectomia, até a conclusão do experimento. No 21º dia após a ovariectomia, os primeiros molares foram extraídos. As ratas foram sacrificadas nos dias 10, 20 e 30 após extrações. Foram avaliadas a cicatrização óssea (BH), células positivas para TRAP e coloração imuno-histoquímica para OPG, RANKL, BSP, OPN e OCN. O grupo Ovariectomia apresentou BH diminuída em comparação ao grupo LiCl aos 10 dias e a menor BH aos 20 dias (p<0,05). Aos 30 dias, os grupos Ovariectomia e LiCl apresentaram menor BH do que o Controle (p<0,05). O número de células positivas para TRAP foi menor no grupo ClLi em 20 dias e o maior no grupo Ovariectomia em 30 dias (p<0,05). Aos 10 dias de cicatrização, o grupo ClLi demonstrou imunomarcação mais intensa em todos os marcadores testados quando comparado aos outros grupos, enquanto o grupo Ovariectomia apresentou maior expressão de RANKL do que a do controle (p<0,05). O ClLi melhorou a cicatrização óssea em ratos com deficiência de estrogênio, particularmente nas fases iniciais do reparo. No entanto, como os dados sobre os efeitos do cloreto de lítio no tecido ósseo ainda são preliminares, mais estudos relacionados à sua toxicidade e protocolo de administração são necessários antes de sua aplicação na prática clínica.

Abstract The purpose of this investigation was to evaluate the effects of lithium chloride (LiCl) on the socket healing of estrogen-deficient rats. Seventy-two rats were allocated into one of the following groups: Control, Ovariectomy and LiCl (150 mg/kg/2 every other day orally) + Ovariectomy. Animals received LiCl or water from the 14th day post-ovariectomy, until the completion of the experiment. On the 21st day after ovariectomy, the first molars were extracted. Rats were euthanized on the 10th, 20th and 30th days following extractions. Bone healing (BH), TRAP positive cells and immunohistochemical staining for OPG, RANKL, BSP, OPN and OCN were evaluated. The Ovariectomy group presented decreased BH compared to the LiCl group at 10 days, and the lowest BH at 20 days (p<0.05). At 30 days, the Ovariectomy and LiCl-groups presented lower BH than that of the Control (p<0.05). The number of TRAP-stained cells was the lowest in the LiCl group at 20 days and the highest in the Ovariectomy group at 30 days (p<0.05). At 10 days of healing, the LiCl group demonstrated stronger staining for all bone markers when compared to the other groups, while the Ovariectomy group presented higher RANKL expression than that of the Control (p<0.05). LiCl enhanced bone healing in rats with estrogen deficiency, particularly in the initial healing phases. However, as data on the effects of lithium chloride on bone tissue are still preliminary, more studies related to its toxicity and protocol of administration are necessary before its application in clinical practice.

https://doi.org/10.1590/0103-6440202003595 733 downloads

Thermographic profile of soccer players' lower limbs
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Bouzas Marins, J. C.

; Andrade Fernandes, A. de

; Gomes Moreira, D.

; Souza Silva, F.

; Magno A. Costa, C.

; Pimenta, E. M.

; Sillero-Quintana, M.

Revista Andaluza de Medicina del Deporte

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mar 2014, Volume 7 N. 1 Pages 1 - 6

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Objective. The objective of this study was to establish the thermographic profile of the lower limbs in elite young soccer players. Method. One hundred soccer players from the U-19 categories of a first division Brazilian football club (15.5 ± 1.37 years; 67.93 ± 9.62 kg; 177.49 ± 8.67 cm) participated in the study. Two thermograms allowed us to record maximum and average skin temperatures (T SK) in four body regions of interest (ROIs) of the lower limbs corresponding to the anterior and posterior view of the leg and thigh. The Wilcoxon test was used to compare bilateral T SK differences with a significance level of α < 0.05. Results. Average values of T SK in the anterior view were as follows: right thigh 30.2 ± 1.9°C, left thigh 30.2 ± 1.9°C, right leg 29.8 ± 1.8°C, and left leg 29.9 ± 1.8°C. In the posterior view, the values were as follows: right thigh 30.3 ± 1.8°C, left thigh 30.2 ± 1.8°C, right leg 29.6 ± 1.9°C, and left leg 29.4 ± 1.9°C. The statistical analysis did not show significant differences between sides in the selected ROIs for average or maximum temperatures. A histogram of T SK frequencies for each ROI allowed establishment of values for hyper-and hypothermia. Conclusion. The elite young soccer players analyzed showed contralateral thermal symmetry. The average T SK differences for paired ROIs were each < 0.2°C. Each ROI exhibited a specific thermal profile. The registered T SK indicated a normal thermal profile of the athletes.

Objetivo. El objetivo de este estudio es establecer el perfil termográfico de los miembros inferiores en jóvenes jugadores de fútbol de élite. Método. En el estudio participaron 100 jugadores de fútbol de categorías sub-19 de clubes de fútbol brasileños de primera división (15,5 ± 1,37 años; 67,93 ± 9,62 kg; 177,49 ± 8,67 cm). Mediante dos termogramas se obtuvieron las temperaturas máximas y medias de la piel (T SK) de cuatro regiones corporales de interés (RDI) correspondientes a la vista anterior y posterior de la pierna y del muslo. Se empleó el test de Wilcoxon para comparar las diferencias de la T SK bilateral, con un nivel de significación α < 0,05. Resultados. Los valores medios de la T SK en la vista anterior fueron los siguientes: muslo derecho 30,2 ± 1,9°C, muslo izquierdo 30,2 ± 1,9°C, pierna derecha 29,8 ± 1,8°C y pierna izquierda 29,9 ± 1,8°C. En la vista posterior, los valores fueron los siguientes: muslo derecho 30,3 ± 1,8°C; muslo izquierdo 30,2 ± 1,8°C; pierna derecha 29,6 ± 1,9°C y pierna izquierda 29,4 ± 1,9°C. El análisis estadístico no mostró diferencias significativas en las temperaturas medias o máximas tomadas en las RDI elegidas. Un histograma de las frecuencias de T SK para cada RDI permitió establecer valores para hiper e hipotermia. Conclusión. Los jóvenes jugadores de fútbol de élite analizados mostraron simetría térmica contralateral. La T SK media para pares de RDI era para cada uno < 0,2°C. Cada RDI mostró un perfil térmico específico. La T SK mostró un perfil térmico normal de los atletas.

495 downloads

A glass bead protocol for recovery of host cell free Ehrlichia canis and quantification by Sybr-green real-time PCR
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Cardozo, G. P.

; Santos, E. V.

; Fachin, A. L.

; França, S. C.

; Marins, M.

Biocell

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apr 2011, Volume 35 N. 1 Pages 35 - 36

Abstract: En | Text: En | PDF: En

E. canis infection of the canine cell line DH82 is a routine in studies with this bacteria. A protocol for isolation of host cell free bacteria was developed based on the use of glass beads. Improvement of infection with E. canis isolated by this method was detected by real-time PCR.

282 downloads

Comparação entre distribuições de referência para a classificação do estado nutricional de crianças e adolescentes com síndrome de Down
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Lopes, Taís de S.

; Ferreira, Daniele M.

; Pereira, Rosangela A.

; Veiga, Gloria V. da

; Marins, Vania M. R. de

Jornal de Pediatria

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aug 2008, Volume 84 N. 4 Pages 350 - 356

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: Avaliar a concordância da categorização dos índices peso para idade (P/I) e estatura para idade (E/I) em indivíduos com síndrome de Down segundo diferentes curvas. MÉTODOS: Desenvolveu-se estudo transversal em crianças (2 a 9,9 anos de idade) e adolescentes (10 a 17,9 anos de idade) com síndrome de Down assistidos em instituições da região metropolitana do Rio de Janeiro. Os índices de P/I e E/I foram categorizados segundo os percentis de três curvas: duas para indivíduos com síndrome de Down e outra para indivíduos saudáveis. Os limites utilizados na categorização foram o percentil 5 (P5) e o percentil 95 (P95). Utilizou-se o Kappa ponderado na avaliação da concordância das classificações (significativo quando p < 0,05). RESULTADOS: Foram obtidas informações de 98 crianças e 40 adolescentes. Entre as crianças, o P/I < P5 variou de 1,0 a 18,4%; a concordância para essa categorização foi considerada fraca (Kappa = 0,16; IC95% -0,03-0,34; p < 0,01); para E/I, não foi observada concordância na classificação. Entre os adolescentes, a categorização do P/I < P5 variou de 2,5 a 5,0%; a concordância para essa classificação foi fraca (Kappa = 0,16; IC95% -0,15-0,48; p > 0,05); para E/I, a concordância foi boa (Kappa = 1,00; IC95% 0,23-1,00; p < 0,01). CONCLUSÃO: A concordância entre as classificações dos índices P/I e E/I geradas por diferentes distribuições mostrou-se fraca. Os dados indicam que o desenvolvimento de curvas específicas para indivíduos com síndrome de Down seria útil para a identificação de distúrbios ponderais, como o excesso de peso, que é freqüentemente observado nesse grupo.

OBJECTIVE: To compare the agreement between classifications of children and adolescents with Down syndrome (DS) by weight for age (W/A) and height for age (H/A) indexes according to a selection of international reference curves. METHODS: A cross-sectional study was carried out of children (2 to 9.9 years old) and adolescents (10 to 17.9 years old) with DS from cities in the state of Rio de Janeiro, Brazil, in 2005. The W/A and H/A indexes were classified according to the percentiles of two curves developed for individuals with Down syndrome and one distribution developed for healthy subjects. The cut-off limits applied for categorization were: below the 5th percentile (< P5) and above the 95th percentile (> 95). The weighted Kappa index was estimated to assess agreement between the classifications (p < 0.05). RESULTS: Information was obtained on 98 children and 40 adolescents. From 1.0 to 18.4% of the children were < P5 for W/A and the agreement for this index was considered weak (Kappa = 0.16; 95%CI -0.03-0.34; p < 0.01) no agreement was observed between the H/A classifications. For adolescents, W/A < P5 varied from 2.5 to 5.0%; and once more there was no agreement for this classification (Kappa = 0.16; 95%CI -0.15-0.48; p > 0.05). There was good agreement for the H/A index (Kappa = 1.00; 95%CI 0.23-1.00; p < 0.01). CONCLUSION: There was weak agreement between classifications of anthropometric indexes according to three different distributions. The data indicate that the construction of specific curves for individuals with DS would facilitate the identification of overweight, which is often observed among these patients.

12637 downloads

Analysis of the 16S rRNA gene of Anaplasma platys detected in dogs from Brazil
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Cardozo, G.P.

; Oliveira, L.P.

; Zissou, V.G.

; Donini, I.A.N.

; Roberto, P.G.

; Marins, M.

Brazilian Journal of Microbiology

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sep 2007, Volume 38 N. 3 Pages 478 - 479

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A comparação de sequências parciais do gene 16S RNAr de Anaplasma platys detectadas em cães de Ribeirão Preto, Brasil, com sequências de outras linhagens previamente depositadas no GenBank indicam que existem pelo menos três linhagens de A. platys circulando na América do Sul.

Comparison of the partial DNA sequence of the 16S rRNA gene of Anaplasma platys detected in dogs from Ribeirão Preto, Brazil, to sequences of other strains previously deposited in GenBank showed that there are at least three A. platys strains circulating in South America.

2194 downloads Cited 2 times in SciELO

A growth hormone-based phylogenetic analysis of euteleostean fishes including a representative species of the Atheriniformes Order, Odontesthes argentinensis
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Marins, Luis F.

; Levy, Jose A.

; Folch, Josep M.

; Sanchez, Armand

Genetics and Molecular Biology

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2003, Volume 26 N. 3 Pages 295 - 300

Abstract: En | Text: En | PDF: En

The GH (growth hormone) cDNA sequence of the marine silverside fish Odontesthes argentinensis was obtained using the RACE protocol (Rapid Amplification of cDNA Ends). The marine silverside GH cDNA sequence is 928 nucleotides long and was found to encode a polypeptide of 204 amino acids, including a signal peptide of 17 amino acids. The 5' and 3' untranslated regions of the messenger are 109 and 204 nucleotides long, respectively. The deduced GH amino acid sequence was used to infer a phylogenetic tree with GH amino acid sequences from representative species belonging to the Euteleostei Subdivision using the maximum parsimony method. The topology found is according to the major phylogenetic grouping of euteleosts. The results corroborate the hypothesis that atherinids are not related to paracanthopterygians as previously suggested, and show a lack of solid synapomorphies among most of the Acanthopterygii Orders analysed indicating a complex assemblage of fishes in which the phylogenetic tree remains indeterminable.

2588 downloads

10.

Alternativa analítica para especiação físico-química de mercúrio em águas costeiras tropicais
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Marins, Rozane V.

; Paraquetti, Heloísa Helena M.

; Ayres, Gisele A.

Química Nova

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may 2002, Volume 25 N. 3 Pages 372 - 378

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

The determination of the physical-chemical mercury speciation in tropical coastal waters using the derivatization technique with CVAFS detection was optimized. This methodology is able to separate the elementary, reactive, organic and total mercury phases with low operational costs. The importance of bromide chloride solution, the diluted aqua regia, used as the oxidative solution, and different compositions of the reductive solution of stannous chloride were tested and the better results for coastal waters are presented. The recuperation of the sum of the different phases ranges from 80 to 94% of the total Hg content in duplicates of different marine samples. The optimized methodology permits important studies on the mercury cycle in the coastal environment.

6889 downloads

11.

Intercorrências clínicas na Síndrome de Turner
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Guimarães, Marília M.

; Guerra, Carla T.G.

; Alves, Solange T.F.

; Cunha, Maria C.S.A.

; Marins, Luiz A.

; Barreto, Luiz F.M.

; Teich, Evelyn

; Santos, Flávia N.

; Ornellas, José F.R.

; Carakushansky, Gerson

; Pinheiro, Gustavo

; Oliveira, Helena P.

; Beserra, Isabel C.R.

; Ghiaroni, Juraci

; Peixoto, Marcelo V.

; Farias, Maria L.F.

; Lanfredi, Mônica M.A.

; Proença, Munira A.

; Ramalho, Paula P.

; Pinheiro, Pedro S.

; Zagury, Roberto L.

; Antunes, Roberto A.

Arquivos Brasileiros de Endocrinologia & Metabologia

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aug 2001, Volume 45 N. 4 Pages 331 - 338

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Neste trabalho descrevemos as malformações e co-morbidades observadas em pacientes com síndrome de Turner (ST). Foi realizado um estudo retrospectivo, avaliando os prontuários de 60 pacientes cujo diagnóstico de ST foi confirmado através do cariótipo, desde a fase pré-natal até a idade de 49 anos. As pacientes encontram-se com idades entre 1 e 50 anos e foram evoluídas num período de 4 meses a 29 anos. Trinta e uma apresentavam o cariótipo 45XO, 24 eram mosaico e 5 apresentavam o padrão 46Xi, (i,Xq). Todas tinham baixa estatura e algum tipo de estigma. Cinco (8,3%) não apresentavam outras malformações congênitas e eram saudáveis; 55 (91,6%) apresentavam doenças associadas, sendo que em 23 (38,3%) foram detectadas doenças endócrinas, em 16 (26,6%) otorrinolaringológicas, 15 (25%) cardiológicas, 14 (23,3%) nefrológicas e 6 (10%) gastrointestinais. Entre as doenças endócrinas mais comuns, observamos hipotireoidismo (36,6%), seguido de osteoporose (18,3%) e hiperlipemia (11,6%). As doenças otorrinolaringológicas mais comuns foram as infecções (otite média e amigdalite); das doenças cardiológicas, as valvulopatias (principalmente aorta bicúspide), das nefrológicas as duplicações do sistema coletor e rotações renais e das gastrointestinais foram observados dois casos de divertículo de Meckel. Encontramos maior prevalência de malformações cardíacas nas pacientes com cariótipo 45XO, embora sem significância estatística quando considerados os demais cariótipos. Concluímos que, devido à alta ocorrência de doenças nesta síndrome, estas devem ser acompanhadas periodicamente em diferentes especialidades.

A retrospective study was held on data of 60 patients with Turner's Syndrome (TS), aiming to report the most prevalent diseases in this syndrome. Diagnosis was confirmed by kariotype from the prenatal period to the age of 49 years. At the time of the study the age of the patients ranged from 1 to 50 years, and they were observed during 4 months to 29 years. Thirty-one patients presented 45XO kariotype, 24 were mosaic and 5 were 46Xi, (i,Xq). All presented with low stature and some stigmas. Five (8.3%) did not present other diseases and are currently healthy. In 55 (91.6%) the following associate diseases were observed: 23 (38.3%) had endocrine, 16 (26.6%) ear, throat and nose, 15 (25%) cardiologic, 14 (23.3%) renal and 6 (10%) gastrointestinal diseases. The most common endocrine diseases were hypothyroidism (36.6%), osteoporosis (18.3%) and hyperlipidemia (11.6%). The most frequent ear, nose and throat diseases were infections (media otitis and tonsillitis). Congenital diseases of the aorta valve (bicuspid aorta valve) and double collecting systems with renal rotation were the most prevalent cardiac and renal diseases, respectively. Two patients had a diagnosis of Meckel's diverticulum. Although congenital diseases were more common in 45XO patients, no statistical significant difference was found concerning the prevalence of the diseases on other karyotypes. We conclude that special support must be given to patients with TS, with periodical assistance by multiple specialists.

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12.

The effect of abiotic factors on the hatching of Moina micrura Kurz, 1874 (Crustacea: Cladocera) ephippial eggs
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- SciELO Analytics

ROJAS, N. E. T.

; MARINS, M. A.

; ROCHA, O.

Brazilian Journal of Biology

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- Sobre o periódico
- SciELO Analytics

aug 2001, Volume 61 N. 3 Pages 371 - 376

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Foram investigados os efeitos de alguns fatores abióticos na taxa de eclosão dos ovos efipiais de Moina micrura. O conhecimento das melhores condições para a eclosão desses ovos pode ser importante na utilização dessa espécie para a aqüicultura, como alimento para larvas de peixes. Os efípios foram expostos a diferentes experimentos em laboratório por um período de sete dias. As melhores condições de eclosão foram: pH de 5 a 9, temperatura de 25ºC, fotoperíodo de oito ou mais horas de luz e intensidade luminosa igual ou maior que 850 lux. Diferentes concentrações de íons na água (de água deionizada e até 880 mg.L<FONT FACE=Symbol>-</FONT>1 de sais selecionados) não influenciaram a eclosão.

The roles of some abiotic factors in controlling the hatching rates of Moina micrura ephippial eggs were investigated. Determination of optimum hatching conditions would be important in developing the use of this species as a food for fish larvae in aquaculture. Ephippia were exposed to different treatments in the laboratory, and monitored for hatching over a period of seven days. Optimum hatching conditions were: pH 5-9, temperature 25ºC, photoperiod eight or more hours light per day and light intensity equal to or greater than 850 lux. Differences in water ionic concentrations (from deionized water to 880 mg.L<FONT FACE=Symbol>-</FONT>1 of selected salts) had no effect.

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