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Abstract Objective To evaluate clinical characteristics and outcomes of COVID-19 patients infected with HIV, and to compare with a paired sample without HIV infection. Methods This is a substudy of a Brazilian multicentric cohort that comprised two periods (2020 and 2021). Data was obtained through the retrospective review of medical records. Primary outcomes were admission to the intensive care unit, invasive mechanical ventilation, and death. Patients with HIV and controls were matched for age, sex, number of comorbidities, and hospital of origin using the technique of propensity score matching (up to 4:1). They were compared using the Chi-Square or Fisher's Exact tests for categorical variables and the Wilcoxon for numerical variables. Results Throughout the study, 17,101 COVID-19 patients were hospitalized, and 130 (0.76%) of those were infected with HIV. The median age was 54 (IQR: 43.0;64.0) years in 2020 and 53 (IQR: 46.0;63.5) years in 2021, with a predominance of females in both periods. People Living with HIV (PLHIV) and their controls showed similar prevalence for admission to the ICU and invasive mechanical ventilation requirement in the two periods, with no significant differences. In 2020, in-hospital mortality was higher in the PLHIV compared to the controls (27.9% vs. 17.7%; p = 0.049), but there was no difference in mortality between groups in 2021 (25.0% vs. 25.1%; p > 0.999). Conclusions Our results reiterate that PLHIV were at higher risk of COVID-19 mortality in the early stages of the pandemic, however, this finding did not sustain in 2021, when the mortality rate is similar to the control group.

https://doi.org/10.1016/j.clinsp.2023.100223

Cuidados em saúde bucal a crianças e adolescentes com paralisia cerebral: percepção de pais e cuidadores
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Silva, Elizabeth Louisy Marques Soares da

; Góes, Paulo Sávio Angeiras de

; Vasconcelos, Márcia Maria Vendiciano Barbosa

; Jamelli, Silvia Regina

; Eickmann, Sophie Helena

; Melo, Márcia Maria Dantas Cabral de

; Lima, Marília de Carvalho

Ciência & Saúde Coletiva

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oct 2020, Volume 25 N. 10 Pages 3773 - 3784

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Objetivou-se avaliar os cuidados em saúde bucal de crianças/adolescentes com Paralisia Cerebral (PC) segundo a gravidade, através da percepção dos pais/cuidadores. Estudo série de casos realizado em serviços de saúde de Pernambuco com 94 mães/cuidadores de sujeitos com PC entre 5 e 18 anos. Avaliaram-se fatores socioeconômicos, demográficos, cuidados em saúde bucal e uso de serviços odontológicos (SO). O Sistema de Classificação da Função Motora Grossa mostrou 67% com comprometimento motor grave. Indivíduos com PC grave tiveram frequência significantemente maior de pertencerem a famílias com renda mais baixa (89%, p < 0,001), residirem no interior (44%, p < 0,005), terem dificuldades de transporte (60%, p = 0,04) e acessibilidade aos SO (88%, p = 0,009) e maior necessidade de higiene bucal (67%, p = 0,008) realizada pelas mães (94%, p < 0,001). Apesar das barreiras de acesso aos SO, o mesmo foi facilitado para aqueles com PC grave, com uso precoce dos SO, mas com baixa oferta de dentistas e de humanização. Esses resultados informam problemas relacionados aos cuidados diários em saúde bucal, contexto de vida das famílias, de apoio institucional e de qualidade dos SO a serem enfrentados com políticas públicas socioeconômicas e de saúde integrais inclusivas e equânimes.

Abstract The aim was to evaluate the oral health care of children/adolescents with Cerebral Palsy (CP) according to severity through the perceptions of parents/caregivers. A case series study was conducted at health services in the state of Pernambuco, Brazil with 94 mothers/caregivers of subjects with CP from 5 and 18 years old. Sociodemographic factors, oral health care and use of dental services (DS) were evaluated. The Gross Motor Function Classification System showed 67% with severe motor impairment. Subjects with severe CP had significantly higher frequencies of belonging to families with lower income (89%, p < 0.001), living in the interior (44%, p < 0.005), having transportation difficulties (60%, p = 0.04), difficulty regarding access to DS (88%, p = 0.009) and a greater need for oral hygiene (67%, p = 0.008), which was performed exclusively by the caregiver (94%, p < 0.001). Despite identified access barriers, dental care was facilitated for those with severe CP, early DS use, but low availability of dentists and low degree of humanization were cited as major problems. These results reveal problems related to daily oral health care, family living context, institutional support and quality of DS that should be addressed in comprehensive, inclusive, equitable social and economic public policies.

https://doi.org/10.1590/1413-812320202510.27972018 2189 downloads

Cuidados em saúde bucal a crianças e adolescentes com paralisia cerebral: percepção de pais e cuidadores
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Silva, Elizabeth Louisy Marques Soares da

; Góes, Paulo Sávio Angeiras de

; Vasconcelos, Márcia Maria Vendiciano Barbosa

; Jamelli, Silvia Regina

; Eickmann, Sophie Helena

; Melo, Márcia Maria Dantas Cabral de

; Lima, Marília de Carvalho

Ciência & Saúde Coletiva

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oct 2020, Volume 25 N. 10 Pages 3773 - 3784

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1590/1413-812320202510.27972018

Diversity of yeasts during fermentation of cocoa from two sites in the Brazilian Amazon
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ALMEIDA, Silvana de F. Oliveira de

; SILVA, Letícia R Carvalho

; JUNIOR, Gilson Celso A. Chagas

; OLIVEIRA, Guilherme

; SILVA, Silvia Helena Marques da

; VASCONCELOS, Santelmo

; LOPES, Alessandra Santos

Acta Amazonica

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mar 2019, Volume 49 N. 1 Pages 64 - 70

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A proposta deste estudo foi identificar as leveduras envolvidas na fermentação espontânea de cacau da Amazônia brasileira. A fermentação foi realizada em Medicilândia e Tucumã, Pará, Brasil, durante 6 dias. Em total foram obtidos 44 isolados de leveduras de Medicilândia e 29 de Tucumã. A identificação molecular foi realizada por sequenciamento do fragmento da região D1/D2 do gene rRNA 26S, amplificado com primers universais para eucariotos NL1GC e LS2. Em Medicilândia, foram identificadas Pichia manshurica e Saccharomyces cerevisiae. Em Tucumã foram identificadas cinco espécies (Pichia fermentans, P. kudriavzevii, P. manshurica, S. cerevisiae e Zygosaccharomyces bailii). Os resultados sugerem que P. manshurica e S. cerevisiae podem ter potencial para uso como culturas starter em estudos futuros, para melhorar a qualidade das sementes de cacau fermentadas na Amazônia brasileira.

ABSTRACT The purpose of this study was to identify the yeasts involved in spontaneous fermentation of cocoa from the Brazilian Amazon region. The fermentation process was carried out experimentally with cocoa seeds from two sites (Medicilândia and Tucumã), State of Pará, northern Brazil, during a six-day period. Totals of 44 yeasts were isolated from Medicilândia and 29 from Tucumã. Molecular identification was carried out by sequencing the D1/D2 region fragment of the rRNA 26S gene, expanded with universal primers for the NL1GC and LS2 eukaryotes. Pichia manshurica and Saccharomyces cerevisiae were identified in Medicilândia and five yeast species (Pichia fermentans, P. kudriavzevii, P. manshurica, S. cerevisiae and Zygosaccharomyces bailii) were identified in Tucumã. The results showed that P. manshurica and S. cerevisiae may have potential for use as starter cultures in future studies to improve the quality of cocoa seeds fermented in the Brazilian Amazon region.

https://doi.org/10.1590/1809-4392201703712 2448 downloads

Isolation of Candida spp. from denture-related stomatitis in Pará, Brazil
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Gauch, Lurdete Maria Rocha

; Pedrosa, Simone Soares

; Silveira-Gomes, Fabíola

; Esteves, Renata Antunes

; Marques-da-Silva, Silvia Helena

Brazilian Journal of Microbiology

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mar 2018, Volume 49 N. 1 Pages 148 - 151

Abstract: En | Text: En | PDF: En

ABSTRACT The aim of this study was to isolate and identify Candida species from the oral cavity of denture wearers with denture-related stomatitis who were attended at the University Federal of Pará (Belém City, Pará State, Brazil). A total of 36 denture wearers with denture-related stomatitis were included, and type I (50%), type II (33%) and type III (17%) stomatitis were observed. Candida spp. were isolated from 89% of the cases and included five different Candida species. C. albicans was the most frequently recovered species (78% of the cases), followed by C. famata and C. tropicalis. We observed a significant association between Candida species isolation and unsatisfactory denture condition (p = 0.0017). Our results demonstrated the highly frequency of Candida species isolation in denture wearers with denture-related stomatitis and showed the relationship between these species and poor denture maintenance.

https://doi.org/10.1016/j.bjm.2017.07.001 6418 downloads

Histological analysis of the use of biphasic ceramics based on hydroxyapatite and β-tricalcium phosphate in maxillary sinus floor elevation surgery: pilot study
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PADOVAN, Luis Eduardo Marques

; GARCIA E SOUSA, Diego

; PADOVAN, Silvia Helena Marques

; LOPES DE OLIVEIRA, Guilherme José Pimentel

; SILVA, Ricarda Duarte da

; CLAUDINO, Marcela

Revista de Odontologia da UNESP

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mar 2018, Volume 47 N. 2 Pages 63 - 68

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Objetivo Avaliar o reparo ósseo associado ao uso da cerâmica bifásica a base de hidroxiapatita e β-tricálcio fosfato (HA/TCP) nos procedimentos de elevação da membrana do seio maxilar em humanos. Material e método Foram selecionados 10 pacientes com rebordo ósseo residual entre 3 mm e 5 mm de altura na região posterior da maxila e que tinham indicação para procedimentos de enxertia óssea associado ao levantamento de seio maxilar. Os seios maxilares foram preenchidos com HA/TCP, e após 8 meses, foi executado a instalação dos implantes e nesse momento foi realizada a coleta de um fragmento ósseo com auxílio de uma broca trefina, o qual foi submetido à análise histológica da área enxertada. Resultado A análise histológica das biópsias revelou a presença de tecido ósseo vital e imaturo, justaposto ao biomaterial. A análise histomorfométrica revelou percentuais de 28,8%, 27,4% e 43,6% para tecido ósseo, biomaterial e tecido mole, respectivamente. Conclusão As áreas enxertadas com a HA/TCP apresentaram quantidade adequada de formação óssea que permitiu a instalação dos implantes. O sucesso do procedimento de enxertia com esse biomaterial esteve associado ao seu potencial de osteocondução que permitiu a formação de tecido ósseo em íntimo contato com a HA/TCP.

Abstract Objective To evaluate the bone repair associated with the use of biphasic ceramics based on hydroxyapatite and β-tricalcium phosphate (HA/TCP) in the procedures of maxillary sinus membrane elevation in human beings. Material and method Ten patients with a residual bone ridge in the posterior maxillary region between 3 mm and 5 mm in height were selected and indicated for procedures of bone grafting associated with maxillary sinus lift procedure. The maxillary sinuses were filled with HA/TCP, and after 8 months, the implants were implanted and a bone biopsy was collected with the aid of a trephine drill, which was then submitted to histological analysis for the evaluation of the composition of the grafted area. Result The histological description analysis of the biopsies revealed the presence of vital and immature bone tissue, juxtaposed to the biomaterial. Histomorphometric analysis showed that the biopsy composition was in average 28.8%, 27.4%, and 43.6% of bone tissue, biomaterial, and soft tissue, respectively. Conclusion The areas grafted with HA/TCP presented the adequate amount of bone formation that allowed the implantation of the implants. The success of the grafting procedure with this biomaterial was associated with its osteoconduction potential that allowed the formation of bone tissue in close contact with HA/TCP.

https://doi.org/10.1590/1807-2577.10917 1150 downloads

Oral and systemic manifestations in HIV-1 patients
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Menezes, Tatiany Oliveira de Alencar

; Rodrigues, Marília Corrêa

; Nogueira, Brenna Magdalena Lima

; Menezes, Sílvio Augusto Fernandes de

; Silva, Sílvia Helena Marques da

; Vallinoto, Antonio Carlos Rosário

Revista da Sociedade Brasileira de Medicina Tropical

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feb 2015, Volume 48 N. 1 Pages 83 - 86

Abstract: En | Text: En | PDF: En

INTRODUCTION: This study aimed to estimate the prevalence of the most frequent oral and systemic manifestations in human immunodeficiency virus-1 (HIV-1)-positive patients. METHODS: The study was conducted on 300 HIV-1 patients attending the Reference Unit Specialized in Special Infectious Parasitic Diseases in Belém, Pará, Brazil. RESULTS: The most prevalent oral conditions were caries (32.6%), candidiasis (32%), and periodontal disease (17%). Among the systemic manifestations, hepatitis (29.2%), gastritis (16%), arterial hypertension (14.7%), and tuberculosis (12%) were the most commonly observed. CONCLUSIONS: We here reported on the most prevalent oral and systemic conditions in HIV-1-positive patients. The healthcare professional's knowledge of the various manifestations among these patients is fundamental to ensure prompt and accurate diagnosis and treatment, and for improving the quality of life of these patients.

https://doi.org/10.1590/0037-8682-0179-2014 5294 downloads

Relationship among local and functional factors in the development of denture stomatitis in denture wearers in northern Brazil
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GAUCH, Lurdete Maria Rocha

; SILVEIRA-GOMES, Fabíola

; PEDROSA, Simone Soares

; ESTEVES, Renata Antunes

; MARQUES-DA-SILVA, Silvia Helena

Revista de Odontologia da UNESP

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oct 2014, Volume 43 N. 5 Pages 314 - 318

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: O objetivo deste estudo foi avaliar a relação entre fatores funcionais e qualitativos no desenvolvimento da estomatite protética (EP) (de acordo com a classificação de Newton) em usuários de dentadura acrílica residentes no norte do Brasil.MATERIAL E MÉTODO: Um total de 99 pacientes, que usavam dentadura superior de resina acrílica parcial ou total, foi incluído neste estudo. Os participantes preencheram um formulário de dados epidemiológicos, que incluiu gênero, idade, fatores locais (hábitos de higiene, retirar a dentadura ao dormir, uso de colutório, condição atual da dentadura, idade da dentadura) e fatores funcionais (dimensão vertical de repouso, dimensão vertical de oclusão, oclusão, retenção, estabilidade estática e dinâmica). Para detectar leveduras, amostras foram coletadas da superfície interna da dentadura e da mucosa palatal em contato com esta. Posteriormente, as amostras foram cultivadas em ágar Sabouraud dextrose, observando-se características macro e microscópicas.RESULTADO: No presente estudo, não foi encontrada relação significante entre gênero e início da doença. Baseada na classificação de Newton, 36,3% dos pacientes apresentaram EP e 89,0% foram colonizados por leveduras; destes indivíduos, 50% tiveram lesões tipo I, 33,3% tiveram lesões tipo II e 16,6% tiveram lesões tipo III. Todos os fatores locais e qualitativos, exceto o uso de colutório, foram clinicamente relevantes para o desenvolvimento da doença.CONCLUSÃO: Estomatite protética em usuários de dentadura do norte do Brasil foi multifatorial, associando fatores locais, funcionais e microbiológicos.

OBJECTIVE: The aim of this study was to evaluate the relationship among functional and qualitative factors in the development of denture stomatitis (DS) (according to Newton's classification) in acrylic-based denture wearers residents from northern Brazil.MATERIAL AND METHOD: A total of 99 patients who wore partial or total acrylic resin-based upper dentures were included in this study. The subjects completed an epidemiological data form that includes the patient's gender, age, local factors (hygiene habits, remove denture to sleep, use of mouthwash, present condition of the denture, age of the denture) and functional factors (vertical dimension at rest, vertical dimension of occlusion, occlusion, retention, and static and dynamic stability). To detect yeasts, samples were collected from the inner surface of the dentures and from the palatal mucosa in contact with it. Subsequently, the samples were cultured on Sabouraud dextrose agar, observing macro and microscopic characteristics.RESULT: In the present study, we did not find any significant relationship between the gender and disease onset. Based on the Newton classification, 36.3% of the patients presented with DS and 89.0% were colonized by yeasts; of these subjects, 50% had type I lesions, 33.3% had type II lesions, and 16.6% had type III lesions. All of the qualitative and local factors, except the use of mouthwash, were clinically relevant to the development of disease.CONCLUSION: Denture stomatitis in denture users in northern Brazil was multifactorial, involving local, functional and microbiological factors.

https://doi.org/10.1590/rou.2014.050 2091 downloads

Effects of Rosmarinus officinalis essential oil on germ tube formation by Candida albicans isolated from denture wearers
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Gauch, Lurdete Maria Rocha

; Silveira-Gomes, Fabíola

; Esteves, Renata Antunes

; Pedrosa, Simone Soares

; Gurgel, Ely Simone Cajueiro

; Arruda, Alberto Cardoso

; Marques-da-Silva, Silvia Helena

Revista da Sociedade Brasileira de Medicina Tropical

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jun 2014, Volume 47 N. 3 Pages 389 - 391

Abstract: En | Text: En | PDF: En

Introduction The aim of this study was to investigate the effects of Rosmarinus officinalis essential oil on germ tube formation by Candida albicans isolated from denture wearers. Methods Ten C. albicans isolates recovered from denture wearers were tested using 10% fetal bovine serum with or without 4% R. officinalis essential oil. Results The essential oil from R. officinalis completely inhibited germ tube formation in the investigated C. albicans isolates. Conclusions The results demonstrate that the essential oil of R. officinalis modulates C. albicans pathogenicity through its primary virulence factor (i.e., germ tube formation was suppressed).

https://doi.org/10.1590/0037-8682-0137-2013 2145 downloads

10.

Dermatophytosis diagnosed at the Evandro Chagas Institute, Pará, Brazil
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Silveira-Gomes, Fabíola

; Oliveira, Elaina Ferreira de

; Nepomuceno, Lívia Barreto

; Pimentel, Rosiane Ferreira

; Marques-da-Silva, Silvia Helena

; Mesquita-da-Costa, Maurimélia

Brazilian Journal of Microbiology

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2013, Volume 44 N. 2 Pages 443 - 446

Abstract: En | Text: En | PDF: En

Dermatophytosis is caused by a dermatophyte fungus that affects the stratum corneum and keratinized tissue. Dermatophyte fungus has been reported worldwide as the causative agent of dermatophytosis, but the etio-epidemiological aspects of these mycoses in the state of Pará remain unknown. The purpose of this study was to describe the etio-epidemiological profile of dermatophytosis diagnosed in patients at the Evandro Chagas Institute from May 2005 to June 2006. A total of 494 patients were admitted, and their samples were collected, submitted for direct microscopic examination using 20% KOH and cultured in Sabouraud and Mycosel medium. The identification was based in macro and microscopic characteristics. Direct examinations were positive in 13% (66/494) of the patients, and agent isolation by cultivation of the biological sample was successful in 4% (20/494), with a high prevalence of T. mentagrophytes (40%; 8/20). Dermatophytosis was more frequent in women (58%; 38/66). Fifty-two percent (21/38) of the cases were children with an average age of 8 years. The most frequent clinical presentation was Tinea corporis (55%, 36/66). For the cases in which the dermatophyte agent was not isolated, we discuss the factors that may be interfering with isolation. Tinea corporis occurred more frequently observed when T. mentagrophytes and T. rubrum were the major etiologic agents.

2332 downloads Cited 1 time in SciELO

11.

Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis
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Silveira-Gomes, Fabíola

; Marques-da-Silva, Silvia Helena

Memórias do Instituto Oswaldo Cruz

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jun 2012, Volume 107 N. 4 Pages 510 - 512

Abstract: En | Text: En | PDF: En

Paracoccidioidomycosis is diagnosed from the direct observation of the causative agent, but serology can facilitate and decrease the time required for diagnosis. The objective of this study was to determine the influence of serum sample inactivation on the performance of the latex agglutination test (LAT) for detecting antibodies against Paracoccidioides brasiliensis. The sensitivity of LAT from inactivated or non-inactivated samples was 73% and 83%, respectively and the LAT selectivity was 79% and 90%, respectively. The LAT evaluated here was no more specific than the double-immunodiffusion assay. We suggest the investigation of other methods for improving the LAT, such as the use of deglycosylated antigen.

3558 downloads Cited 1 time in SciELO

12.

Differentiation between Candida albicans and Candida dubliniensis using hypertonic Sabouraud broth and tobacco agar
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Silveira-Gomes, Fabíola

; Sarmento, Dayse Nogueira

; Espírito-Santo, Elaine Patrícia Tavares do

; Souza, Nádia de Oliveira

; Pinto, Thifany Mendes

; Marques-da-Silva, Silvia Helena

Revista da Sociedade Brasileira de Medicina Tropical

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aug 2011, Volume 44 N. 4 Pages 457 - 460

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

INTRODUÇÃO: Infecções fúngicas oportunistas em hospedeiros imunocomprometidos são causadas por espécies de Candida, cuja maioria das infecções se deve a Candida albicans. Entretanto, o patógeno emergente Candida dubliniensis demonstra várias características fenotípicas em comum com C. albicans, tais como produção de tubo germinativo e clamidósporos, solicitando atenção por desenvolver resistência in vitro estável ao fluconazol. O objetivo do presente estudo foi avaliar a performance da identificação bioquímica na diferenciação entre C. albicans e Candida dubliniensis, analisando fenotipicamente leveduras previamente identificadas como C. albicans. MÉTODOS: Setenta e oito isolados identificados como C. albicans pelo sistema API ID 32C foram cultivados em ágar Sabouraud dextrose a 30°C por 24-48h e em seguida inoculados em caldo hipertônico Sabouraud e agar tabaco. RESULTADOS: Nossos resultados mostraram que 17 (21,5%) isolados tiveram o crescimento inibido no caldo hipertônico Sabouraud, característica fenotípica inconsistente para C. albicans neste meio de cultura. Entretanto, os resultados observados em ágar tabaco mostraram que somente 9 (11,4%) dos isolados inibidos produziram colônias características de C. dubliniensis (colônias rugosas, marrom-amarelada com fragmentos de hifas e abundantes clamidósporos). CONCLUSÕES: Os resultados obtidos sugerem que este é um instrumento simples para triagem entre leveduras de C. albicans e não-albicans, bem como confirmação de identificação automatizada.

INTRODUCTION: Opportunistic fungal infections in immunocompromised hosts are caused by Candida species, and the majority of such infections are due to Candida albicans. However, the emerging pathogen Candida dubliniensis demonstrates several phenotypic characteristics in common with C. albicans, such as production of germ tubes and chlamydospores, calling attention to the development of stable resistance to fluconazole in vitro. The aim of this study was to evaluate the performance of biochemistry identification in the differentiating between C. albicans and C. dubliniensis, by phenotyping of yeast identified as C. albicans. METHODS: Seventy-nine isolates identified as C. albicans by the API system ID 32C were grown on Sabouraud dextrose agar at 30°C for 24-48h and then inoculated on hypertonic Sabouraud broth and tobacco agar. RESULTS: Our results showed that 17 (21.5%) isolates were growth-inhibited on hypertonic Sabouraud broth, a phenotypic trait inconsistent with C. albicans in this medium. However, the results observed on tobacco agar showed that only 9 (11.4%) of the growth-inhibited isolates produced characteristic colonies of C. dubliniensis (rough colonies, yellowish-brown with abundant fragments of hyphae and chlamydospores). CONCLUSIONS: The results suggest that this method is a simple tool for screening C. albicans and non-albicans yeast and for verification of automated identification.

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13.

Digestive and functional properties of a partially hydrolyzed cassava solid waste with high insoluble fiber concentration
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Raupp, Dorivaldo da Silva

; Rosa, Danielle Amorim

; Marques, Silvia Helena de Paula

; Banzatto, David Ariovaldo

Scientia Agricola

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jun 2004, Volume 61 N. 3 Pages 286 - 291

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

As fecularias e polvilheiras produzem grandes quantidades de bagaço de mandioca. A proposta dessa pesquisa consistiu determinar, em ratos modelo, a propriedade funcional-digestiva do produto alimentício bagaço de mandioca hidrolisado (BMH), um concentrado de fibra alimentar (60,9%, peso seco) que foi obtido a partir do bagaço de mandioca da polvilheira e através de processo de hidrólise enzimática. O BMH produziu efeitos fisiológicos no trato digestivo dos ratos modelos mais acentuados que os efeitos produzidos pelo farelo de trigo (FT). Foram os componentes insolúveis da fibra alimentar do BMH, e não a fração solúvel, os que mais contribuíram para o maior volume e peso das fezes e, por conseguinte, para o maior número de defecações. Por isso, o BMH pode ser usado como fonte alternativa de fibra alimentar para a formulação de alimentos, principalmente os consumidos por indivíduos com a finalidade de regular ou manter normal a funcionalidade digestiva.

Starch factories generate large amounts of cassava solid waste. A small amount is utilized for animal feed but most of it is discharged with deleterious effects to the envirounment. A edible food with a high content of insoluble dietary fiber (60.9%), named "partially hidrolyzed cassava waste" (PHCW), was prepared from industrial cassava solid waste by an enzymatic process. PHCW or wheat bran (WB) were fed to model rats and both promoted digestive function effects, but PHCW produced the greatest effect. The insoluble fiber constituent from PHCW (and not the soluble fiber), promoted the greatest fecal bulking, fecal weight and defecation frequency in rats, as compared to WB. Such results indicate that the partially hydrolyzed cassava waste presents digestive function properties which allow it to be used as an adequate source of insoluble dietary fiber in the formulation of functional food for human nutrition.

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14.

Arraste via fecal de nutrientes da ingestão produzido por bagaço de mandioca hidrolisado
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Raupp, Dorivaldo da Silva

; Marques, Silvia Helena de Paula

; Rosa, Danielle Amorim

; Caldi, Carla Marielle

; Cremasco, Ana Candida Vivan

; Banzatto, David Ariovaldo

Scientia Agricola

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jun 2002, Volume 59 N. 2 Pages 235 - 242

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A ingestão de fibras alimentares pode resultar em arraste de outros nutrientes para as fezes. Bagaço de mandioca produzido por polvilheira foi submetido à hidrólise enzimática para obtenção de um produto mais concentrado em fibra alimentar insolúvel denominado de bagaço de mandioca hidrolisado (BMH). O BMH foi avaliado em ensaios biológicos com ratos Wistar quanto a sua habilidade de arrastar os nutrientes da ingestão para as fezes, tendo como padrão de fibra o farelo de trigo (FT). Acréscimo à dieta base, substituindo igual quantidade de amido, de dose de 5%, 15% ou 25% de BMH promoveu arraste significativo de minerais (globalidade) ou proteína; dose de 15% ou 25% promoveu arraste significativo de lipídeo; e, dose de 25% promoveu arraste significativo de carboidrato digestível. Dose de 25% de FT produziu arraste significativo de minerais (globalidade), proteína ou carboidrato digestível, mas, o lipídeo não foi arrastado. BMH produziu efeito mais pronunciado que FT em arrastar minerais (globalidade) ou proteína para a dose de 5% ou 15%; no entanto, para a dose alta, de 25%, as fontes produziram efeitos semelhantes em arrastar a proteína, mas o FT promoveu maior arraste de minerais (globalidade). Não houve diferença entre fontes, BMH e FT, em arrastar lipídeo ou carboidrato digestível para as fezes. Na dose alta (25%) o BMH promoveu efeitos, no máximo, semelhantes ao FT, em arrastar para as fezes os nutrientes da ingestão; assim, é recomendado como fonte alternativa de fibra alimentar insolúvel para a formulação de alimentos funcionais-digestivos.

Dietary fiber can drag nutrients in faeces. Cassava waste from starch industry can be hydrolyzed by an enzymatic process and give origin to a food with high content of insoluble dietary fibers, named partially hydrolyzed cassava waste (HCW). HCW or standard wheat bran (WB) were fed to model growing rats in order to assess their dragging skill of nutrients from the intake of faeces. Addition of 5%, 15% or 25% HCW, in replacement of starch, promoted a significant dragging proportion for the overall minerals or proteins; addition of 15% or 25% HCW dragged a significant proportion of lipids and the addition of 25% HCW dragged a significant proportion of digestible carbohydrates. Adding 25% WB caused significant dragging of total minerals, protein and digestible carbohydrates, lipids were, however, not dragged with WB treatment. HCW promoted a more pronounced effect as compared to WB in regard to the dragging process of total minerals or proteins for 5% or 15% additions. Nervertheless, for high rates, such as 25% additions, both fibrous sources produced similar effects on the dragging of protein, but WB promoted the greatest dragging process of total minerals. No differences between both fiber sources were observed as to their abilities of dragging lipids or digestible carbohydrates into faeces. With high rates (25%) HCW promoted effects similar to WB, allowing to conclude that HCW might be used as an important alternative source of insoluble dietary fiber for the formulation of digestive-functional feeds.

2838 downloads Cited 4 times in SciELO

15.

SUBCUTANEOUS PHEOHYPHOMYCOSIS CAUSED BY Phoma cava: REPORT OF A CASE AND REVIEW OF THE LITERATURE
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ZAITZ, Clarisse

; HEINS-VACCARI, Elisabeth Maria

; FREITAS, Roseli Santos de

; ARRIAGADA, Giovana Letícia Hernández

; RUIZ, Ligia

; TOTOLI, Silvia A.S.

; MARQUES, Ana Cristina

; REZZE, Gisele G.

; MÚLLER, Helena

; VALENTE, Neuza Sakai

; LACAZ, Carlos da Silva

Revista do Instituto de Medicina Tropical de São Paulo

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jan 1997, Volume 39 N. 1 Pages 43 - 48

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O presente trabalho registra um caso de feo-hifomicose subcutânea em paciente do sexo masculino com o diagnóstico de sarcoidose pulmonar, submetido à terapêutica por corticosteróides quando apresentou no dorso da mão direita lesões cutâneas nodulares, eritêmato-violáceas, de aspecto infiltrado, exigindo biópsia para o diagnóstico. O exame histopatológico revelou processo granulomatoso, com a presença de hifas e células arredondadas demácias. Cultivo positivo para fungo identificado com Phoma cava. Os Autores fizeram revisão da literatura sobre as infecções fúngicas provocadas por diversas espécies de Phoma, mostrando a raridade desta observação. A evolução foi favorável com a administração da anfotericina B (via venosa) seguida do itraconazol (via oral)

We report a case of subcutaneous pheohyphomycosis observed in a male patient presenting pulmonary sarcoidosis and submitted to corticosteroid treatment. He presented nodular erythematous-violaceous skin lesions in the dorsum of the right hand. Histopathological examination of the biopsied lesion revealed dematiaceous hyphae and yeast-like cells, with a granulomatous tissual reaction. The isolated fungus was identified as Phoma cava. A review of the literature on fungal infection caused by different Phoma species, is presented. The patient healed after therapy with amphotericin B, followed by itraconazole

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