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au:MEDEIROS, M.S.

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Antimicrobial potential of Copaiba Oil (Copaifera multijuga Hayne-Leguminosae) against bubaline mastitis multiresistant isolates
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OLIVEIRA, DIVÂNIA F.F. DE

; NASCIMENTO, THIAGO P.

; RODRIGUES, CLÁUDIO HENRIQUE

; BATISTA, JUANIZE M.S.

; LIU, TATIANA P.S.L.

; MEDEIROS, ELIZABETH S. DE

; MOTA, RINALDO A.

; COSTA, ROMERO MARCOS P.B.

; PORTO, TATIANA S.

; PORTO, CAMILA S.

; PORTO, ANA LÚCIA F.

Anais da Academia Brasileira de Ciências

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2020, Volume 92 N. 4 elocation e20200521

Abstract: En | Text: En | PDF: En

Abstract Copaiba oil is a natural product used by Amazonian populations and recognized for its medicinal properties because it has significant antimicrobial activity for several pathogenic microorganisms. The present work aimed to evaluate and characterize the effect of natural oil produced by copaiba – Copaifera multijuga against multiresistant isolates of bubaline mastitis. The nitrocefin test was performed with isolates of Staphylococcus aureus from bubaline mastitis, which were 100% positive for beta-lactamase enzyme detection. Minimum Bactericidal Concentration (MBC) of 25% to 3.12% was obtained for Enterococcus faecalis and Escherichia coli and 50% and 25% for S. aureus, but Klebsiella pneumoniae and Bacillus subtilis were resistant. MBC with 12.5% and 6.25% oil were obtained for most multiresistant bubaline mastitis isolates from the states of Pernambuco, Ceará, Bahia and Alagoas. The results demonstrated the great potential of using copaiba natural oil in the treatment of buffalo mastitis.

https://doi.org/10.1590/0001-3765202020200521 458 downloads

High-throughput phenotyping of brachiaria grass seeds using free access tool for analyzing X-ray images
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MEDEIROS, ANDRÉ D. DE

; SILVA, LAÉRCIO J. DA

; PEREIRA, MÁRCIO D.

; OLIVEIRA, ARIADNE M.S.

; DIAS, DENISE C.F.S.

Anais da Academia Brasileira de Ciências

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2020, Volume 92 elocation e20190209

Abstract: En | Text: En | PDF: En

Abstract New approaches based on image analysis can assist in phenotyping of biological characteristics, serving as support for decision-making in modern agribusiness. The aim of this study was to propose a method of high-throughput phenotyping of free access for processing of 2D X-ray images of brachiaria grass (Brachiaria ruziziensis cv. Ruziziensis) seeds, as well as correlate the parameters linked to the physiological potential of the seeds. The study was carried out by means of automated analysis of X-ray images of seeds in which a macro, called PhenoXray, was developed, responsible for digital image processing, for which a series of descriptors were obtained. After the X-ray analysis, a germination test was performed on the seeds and, from this, variables related to the physiological quality of the seeds were obtained. The use of the macro PhenoXray allowed large-scale phenotyping of seed X-rays in a simple, rapid, robust, and totally free manner. This study confirmed that the methodology is efficient for obtaining morphometric data and tissue integrity data in Brachiaria ruziziensis seeds and that parameters such as relative density, integrated density, and seed filling are closely related to the physiological attributes of seed quality.

https://doi.org/10.1590/0001-3765202020190209 1000 downloads

Where do we aspire to publish? A position paper on scientific communication in biochemistry and molecular biology
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Baptista, M.S.

; Alves, M.J.M.

; Arantes, G.M.

; Armelin, H.A.

; Augusto, O.

; Baldini, R.L.

; Basseres, D.S.

; Bechara, E.J.H.

; Bruni-Cardoso, A.

; Chaimovich, H.

; Colepicolo Neto, P.

; Colli, W.

; Cuccovia, I.M.

; Da-Silva, A.M.

; Di Mascio, P.

; Farah, S.C.

; Ferreira, C.

; Forti, F.L.

; Giordano, R.J.

; Gomes, S.L.

; Gueiros Filho, F.J.

; Hoch, N.C.

; Hotta, C.T.

; Labriola, L.

; Lameu, C.

; Machini, M.T.

; Malnic, B.

; Marana, S.R.

; Medeiros, M.H.G.

; Meotti, F.C.

; Miyamoto, S.

; Oliveira, C.C.

; Souza-Pinto, N.C.

; Reis, E.M.

; Ronsein, G.E.

; Salinas, R.K.

; Schechtman, D.

; Schreier, S.

; Setubal, J.C.

; Sogayar, M.C.

; Souza, G.M.

; Terra, W.R.

; Truzzi, D.R.

; Ulrich, H.

; Verjovski-Almeida, S.

; Winck, F.V.

; Zingales, B.

; Kowaltowski, A.J.

Brazilian Journal of Medical and Biological Research

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2019, Volume 52 N. 9 elocation e8935

Abstract: En | Text: En | PDF: En

The scientific publication landscape is changing quickly, with an enormous increase in options and models. Articles can be published in a complex variety of journals that differ in their presentation format (online-only or in-print), editorial organizations that maintain them (commercial and/or society-based), editorial handling (academic or professional editors), editorial board composition (academic or professional), payment options to cover editorial costs (open access or pay-to-read), indexation, visibility, branding, and other aspects. Additionally, online submissions of non-revised versions of manuscripts prior to seeking publication in a peer-reviewed journal (a practice known as pre-printing) are a growing trend in biological sciences. In this changing landscape, researchers in biochemistry and molecular biology must re-think their priorities in terms of scientific output dissemination. The evaluation processes and institutional funding for scientific publications should also be revised accordingly. This article presents the results of discussions within the Department of Biochemistry, University of São Paulo, on this subject.

https://doi.org/10.1590/1414-431x20198935 1769 downloads

Primeiro registro de Amblyomma geayi (Acari: ixodidae) em preguiça (Bradypus variegatus) no estado do Acre, Amazônia Ocidental: relato de caso
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Souza, S.F.

; Medeiros, L.S.

; Oliveira, R.S.

; Deschk, M.

; Carvalho, Y.K.

; Ribeiro, V.M.F.

; Souza, A.P.

; Lavina, M.S.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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aug 2016, Volume 68 N. 4 Pages 953 - 957

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O Amblyomma geayi é um carrapato da família Ixodidae, encontrado principalmente em mamíferos e aves silvestres de regiões tropicais da América do Sul. Descreve-se a ocorrência de um carrapato ixodídeo da espécie A. geayi, encontrado em uma preguiça (Bradypus variegatus) proveniente do Parque Zoobotânico da Universidade Federal do Acre, Rio Branco, Acre, Amazônia Ocidental.

ABSTRACT Amblyomma geayi is a tick of the Ixodidae family found primarily in mammals and wild birds from tropical regions in South America. This case report the occurrence of an ixodid tick species A. geayi found in a brown-throated sloth (Bradypus variegatus) from the botanical zoo Park of the Federal University of Acre, Rio Branco, Acre, Western Amazon.

https://doi.org/10.1590/1678-4162-8624 2024 downloads

Genetic analysis of mecA gene and detection of homologue pbpD in Stahylococcus sciuri group
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Calazans-Silva, Amanda C.

; Medeiros, Pedro T.C.

; Araujo, Dayanne M.

; Carvalho, Bruno O.

; Coelho, Irene S.

; Coelho, Shana M.O.

; Souza, Miliane M.S.

Brazilian Journal of Microbiology

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jun 2014, Volume 45 N. 2 Pages 651 - 655

Abstract: En | Text: En | PDF: En

Oxacillin/methicillin-resistance is related to the mecA and its regulatory genes mecR1 and mecI. Its origin is still unknown, although evidences support that it is related to CNS, once mecA and a homologue gene, pbpD, were both detected in Staphylococcus sciuri species group. The present work evaluated 210 samples of skin and ear swabs from rodents and 60 nasal swabs from equines of Army Biologic Institute, Rio de Janeiro. Pheno- and genotypic characterization provided 59.52% (25/42) and 78.57% (11/14) S. lentus and S. sciuri, respectively. It was observed that although all S. sciuri isolates tested positive for pbpD, there was no correlation with oxacillin-resistance. On the other hand, isolates tested positive for mecA gene also presented phenotypic oxacillin-resistance in at least one assay. The alignment of the mecA gene showed that the nucleotide sequences were sorted into 2 different groups, one comprising the bovine strains and the other containing human and equine strains.

1421 downloads

Efeito do sítio de venopunção nos parâmetros hematológicos em tigre-d'água-americano, Trachemys scripta elegans
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Medeiros, Nina C.

; Locatelli-Dittrich, Rosangela

; Schmidt, Elizabeth M.S.

; Alvares, Alessandra A.A.

; Patrício, Lia L.

; Lange, Rogério R.

; Souza, Rodrigo A.M. de

Pesquisa Veterinária Brasileira

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dec 2012, Volume 32 Suppl. 1 Pages 37 - 40

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O objetivo do presente estudo foi realizar a comparação entre dois sítios de coleta sanguínea em 24 exemplares de tigre-d'água-americano (Trachemys scripta elegans) oriundos de um criadouro comercial, localizado no município de Antonina, litoral do Paraná, Brasil. Os animais foram submetidos a contenção física e as venopunções foram realizadas no seio supraocciptal e na veia coccígea dorsal. As amostras heparinizadas foram identificadas e refrigeradas para posterior análise laboratorial. A contagem total de eritrócitos e leucócitos foi realizada pela técnica de hemocitometria. O hematócrito (Ht) e a hemoglobina (Hb) foram determinados pelo método de microhematócrito e cianometahemoglobina, respectivamente. A proteína plasmática total (PPT) foi determinada por refratometria e a contagem diferencial de leucócitos foi realizada através da técnica de Shilling. Houve diferença significativa no número de leucócitos e no valor da proteína plasmática total, e em ambos os casos os valores encontrados nas amostras provenientes da veia coccígea dorsal foram inferiores. A diferença encontrada no número de leucócitos provavelmente foi devido à contaminação por linfa, que também justifica o menor valor na concentração da proteína plasmática total. Conclui-se que é mais indicada a venopunção no seio supraocciptal quando comparado a veia coccígea dorsal.

The aim of the present study was to compare two different blood collection sites of 24 red-eared sliders (Trachemys scripta elegans) from a commercial breeder, situated in Antonina, Paraná, Brasil. The animals were physically restrained and paired blood samples were collected from the dorsal coccygeal vein and the occipital sinus. The samples were collected in syringes containing heparin. After collection the samples were identified and refrigerated to posterior hematological analysis. Red blood cell and white blood count were performed using a hemocytometer. The packed cell volume and hemoglobin were determined by the microhematocrit method and cyanmethemoglobin method, respectively. Total plasma protein was determined by refractometry and differential leukocyte count was performed by the Shilling technique. There was a significant difference in the total white blood cell count and on the total plasma protein value, and on both cases the results found on the samples from the dorsal coccygeal vein were lower. The difference found on the white blood cell count was probably due to the lymph contamination of the samples, which also justifies the lower values of total plasma protein. In conclusion, the occipital sinus is more recommended blood collection site than the dorsal coccygeal vein.

3224 downloads

Efeito do feno de flor-de-seda sobre a carcaça e constituintes corporais de cordeiros Morada Nova
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Silva, N.V. da

; Costa, R.G.

; Medeiros, A.N. de

; Azevedo, P.S. de

; Carvalho, F.F.R. de

; Medeiros, G.R. de

; Madruga, M.S.

Archivos de Zootecnia

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mar 2012, Volume 61 N. 233 Pages 63 - 70

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

The objective of this study was to evaluate the use of silk flower (Calotropis procera SW) hay in 32 castrate male lambs of Morada Nova breed, in feedlot, fed with diets containing 0.0, 15.0, 30.0, and 45.0% of silk flower hay (SFH). The animals (12.72±1.99 kg BW) were slaughtered at 19.29±2.25 kgBW. A completely randomized design, with four diets and eigth replicates, was used. Increasing SFH levels reduced carcass conformation (2.31, 2.58, 2.17 and 1.81 points), leg perimeter (33.38, 32.17, 32.17, 30.00 cm), carcass compacity (live weigth/carcass length) (0.18, 0.18, 0.17, 0.15 cm), corrected half carcass weight (4.52, 4.63, 4.43, 3.69 kg), loin weight (0.66, 0.69, 0.64, 0.50 kg), shoulder weight (0.83, 0.86, 0.80, 0.67 kg) and rib weight (1.13, 1.09, 1.05, 0.84 kg), while there was a linear increase in leg weight (30.98, 30.81, 31.60, 34.47 kg) and mesenteric fat percentage (1.02, 0.65, 1.17, 1.42%). Increasing dietary levels of SFH to lambs affected carcass measurements and carcass yield, retail cuts and non-carcass components. It is thought that the inclusion of 30% of SFH in the diet is excellent for the maintenance of carcass characteristics in regions where there is access to this fodder plant.

Avaliou-se a utilização do feno de flor-deseda (Calotropis procera SW) (FFS) em cordeiros Morada Nova em confinamento. Utilizaram-se 32 cordeiros com peso vivo inicial de 12,72±1,99 kg e de abate 19,29±2,25 kg, distribuídos em delineamento inteiramente casualizado com 4 tratamentos (0%, 15%, 30% e 45% de FFS) e oito repetições. O aumento dos níveis de FFS diminuiu a conformação da carcaça (2,31; 2,58; 2,17; 1,81 pontos), perímetro da coxa (33,38; 32,17; 32,17; 30,00 cm), compacidade da carcaça (0,18; 0,18; 0,17; 0,15 cm), meia carcaça reconstituída (4,52; 4,63; 4,43; 3,69 kg), lombo (0,66; 0,69; 0,64; 0,50 kg), paleta (0,83; 0,86; 0,80; 0,67 kg) e costela (1,13; 1,09; 1,05; 0,84 kg), mas aumentou o linearmente os pesos da perna (30,98; 30,81; 31,60; 34,47 kg) e a gordura mesentérica (1,02; 0,65; 1,17; 1,42%). A inclusão de FFS na dieta promoveu efeito sobre as medidas da carcaça e seus rendimentos, assim como o rendimento dos cortes comerciais e componentes não-constituintes da carcaça. Estima-se que a inclusão de até 30% de FFS na dieta é relevante na manutenção das boas características de carcaça em regiões onde se tenha oferta desta forragem.

https://doi.org/10.4321/S0004-05922012000100007 388 downloads

Efeitos do treinamento funcional com cargas sobre a composição corporal: Um estudo experimental em mulheres fisicamente inativas
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Pereira, P.C.

; Medeiros, R.D.

; Santos, A.A.

; Oliveira, L.S.

; Aniceto, R.R.

; Júnior, A.A.

; Nascimento, J.A.

; Sousa, M.S.

Motricidade

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2012, Volume 8 N. 1 Pages 42 - 52

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O presente estudo objetivou analisar os efeitos do treinamento funcional com cargas (TFC) na composição corporal de mulheres fisicamente inativas. Vinte mulheres (25.70 ± 4.20 anos) foram designadas aleatoriamente para os grupos experimental (GE, n = 10) e controle (GC, n = 10), tendo o GE realizado 12 semanas de TFC, enquanto o GC não recebeu qualquer intervenção. As variáveis antropométricas avaliadas em ambos os grupos foram: massa corporal (MC), estatura, índice de massa corporal (IMC), massa de gordura absoluta, massa corporal magra (MCM) e percentual de gordura (%G). Os testes t de Student independente e pareado foram utilizados para a comparação das médias inter e intra-grupos experimental e controle (p < .05). O GE apresentou reduções significativas nos valores de %G (p < .001), enquanto o GC aumentou seus níveis de MCM (p < .001), IMC (p < .001) e MC (p = .021). Os resultados indicaram que após de 12 semanas o TFC reduz os níveis de gordura corporal, mas não promove aumentos de MCM em mulheres fisicamente inativas.

This study aimed to analyze the effects of the functional strength training (FST) on body composition of physically inactive women. Twenty women aged between 19 and 34 years old (25.70 ± 4.20 yr.) were randomly assigned to experimental (EG, n = 10) and control group (CG, n = 10). The EG performed 12 weeks of FST, while CG did not receive any intervention. The anthropometric variables evaluated in both groups were: body mass (BM), height, body mass index (BMI), absolute fat mass (AFM), lean body mass (LBM) and fat percent (%F). The paired and independent samples t tests were used to identify modifications between and within experimental and control groups (p < .05). The EG decreased %F values (p < .001), while CG increased the LBM (p < .001), BMI (p < .001) and BM (p = .021). Results showed that after 12 weeks FST can be useful to reduce body fat mass, but it doesn’t promote increases on LBM of physically inactive women.

https://doi.org/10.6063/motricidade.8(1).238 25402 downloads Cited 1 time in SciELO

Armazenamento de sementes de Myracrodruon urundeuva Fr. All. em diferentes embalagens e ambientes
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Guedes, R.S.

; Alves, E.U.

; Bruno, R.L.A.

; Gonçalves, E.P.

; Costa, E.G.

; Medeiros, M.S.

Revista Brasileira de Plantas Medicinais

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2012, Volume 14 N. 1 Pages 68 - 75

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Myracrodruon urundeuva Fr. All. é uma espécie pertencente à família Anacardiaceae, cuja planta pode ser utilizada como medicinal, na indústria de curtimento de couro, na arborização de ruas e praças, produzindo madeira de grande resistência mecânica. Diante disso, o objetivo deste trabalho foi determinar as melhores condições e períodos de armazenamento para conservação da viabilidade e vigor das sementes de M. urundeuva. As embalagens utilizadas para acondicionamento das sementes foram sacos de papel Kraft, algodão e de polietileno transparente, bem como folhas de papel alumínio. Em seguida as sementes foram armazenadas em ambiente natural de laboratório (25 ± 2ºC), freezer (-20 ± 2ºC), câmara fria (8 ± 2ºC) e geladeira (6 ± 2ºC). Em intervalos pré-determinados (0, 30, 60, 90, 120, 150, 180, 210 e 240 dias) avaliou-se o teor de água das sementes, porcentagem de emergência, índice de velocidade de emergência, comprimento e massa seca de plântulas. No ambiente de laboratório não houve conservação do vigor das sementes de M. urundeuva. A melhor condição para conservação de sementes de M. urundeuva foi obtida com o acondicionamento em sacos de papel Kraft, pano de algodão, plástico ou papel alumínio e manutenção em geladeira ou freezer, podendo também ser conservadas embaladas em papel ou alumínio, quando estocadas em câmara fria, por 240 dias. Alteração no vigor de sementes dessa espécie é primeiramente identificada pela redução da velocidade de emergência.

Myracrodruon urundeuva Fr. All. is a species belonging to the Anacardiaceae family, which can be utilized as a medicinal plant, or in the leather tanning industry, urban afforestation, and the production of wood with great mechanic resistance. The objective of this study was to determine the best conditions and storage periods for preserving the viability and vigor of M. urundeuva. Seeds conditioned in bags of Kraft paper, cotton, polyethylene or aluminum foil of mesh polyethylene were stored at room temperature (25 ± 2ºC), freezer (-20 ± 2ºC), cold (8 ± 2ºC) and refrigerator (6 ± 2ºC). At 30, 60, 90, 120, 150, 180, 210 and 240 days they were evaluated for seed moisture content, percentage of emergence, emergence speed index (ESI), seedling length and seedling dry matter. In the natural environment of the laboratory, there was no conservation of seed vigor M. urundeuva. The best M. urundeuva seed conservation condition was obtained with bags of Kraft paper, cotton, polyethylene or aluminum foil of mesh polyethylene in the refrigerator or freezer. M. urundeuva seeds can also be kept in paper bags or aluminum foil when stored in a refrigerated chamber, for 240 days. Seed vigor change in this species is initially identified by decrease in the speed emergence.

3409 downloads Cited 9 times in SciELO

10.

Angiotensin-II-induced reactive oxygen species along the SFO-PVN-RVLM pathway: implications in neurogenic hypertension
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Braga, V.A.

; Medeiros, I.A.

; Ribeiro, T.P.

; França-Silva, M.S.

; Botelho-Ono, M.S.

; Guimarães, D.D.

Brazilian Journal of Medical and Biological Research

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sep 2011, Volume 44 N. 9 Pages 871 - 876

Abstract: En | Text: En | PDF: En

Neurogenic hypertension has been the subject of extensive research worldwide. This review is based on the premise that some forms of neurogenic hypertension are caused in part by the formation of angiotensin-II (Ang-II)-induced reactive oxygen species along the subfornical organ-paraventricular nucleus of the hypothalamus-rostral ventrolateral medulla pathway (SFO-PVN-RVLM pathway). We will discuss the recent contribution of our laboratory and others regarding the mechanisms by which neurons in the SFO (an important circumventricular organ) are activated by Ang-II, how the SFO communicates with two other important areas involved in sympathetic activity regulation (PVN and RVLM) and how Ang-II-induced reactive oxygen species participate along the SFO-PVN-RVLM pathway in the pathogenesis of neurogenic hypertension.

25175 downloads

11.

Distribuição do metal mercúrio em atum (Thunnus albacares) e pescada bicuda (Cynoscion microlepidotus) capturados no litoral do Rio de Janeiro, Brasil
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Medeiros, R.J.

; Mársico, E.T.

; São Clemente, S.C.

; Ferreira, M.S.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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jun 2008, Volume 60 N. 3 Pages 656 - 662

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O presente estudo verificou a cinética mercurial em órgãos, tecido muscular e conteúdo estomacal de duas espécies de peixes marinhos predadores, identificando os locais de maior acumulação desse metal. Para o processo analítico, foi utilizada a técnica de espectrofotometria de absorção atômica por arraste de vapor a frio. A distribuição de Hg nas porções analisadas apresenta ampla dispersão. Para o atum, a variação foi de 0,004 µg.g-1 no fígado, nas brânquias e baço, e 0,172µg.g-1 no tecido muscular. Para a pescada bicuda, a variação foi entre 0,013µg.g-1 nas brânquias a 0,250µg.g-1 no tecido muscular. Este último demonstrou ser, nas duas espécies, a porção de maior concentração do metal, atum = 0,080±0,050µg.g-1 e pescada bicuda = 0,187±0,030µg.g-1. Os locais de menor concentração na pescada foram as brânquias 0,029±0,031µg.g-1, e no atum os olhos (0,013±0,008µg.g-1).

The mercuric kinetic in organs, muscular tissue, and stomach contents of two species of marine fish predators was evaluated by the identification of the places showing higher accumulation of the metal. For the analytical process, the used technique was cold vapor atomic absorption spectrometry. The results demonstrated that the distribution of Hg in the analyzed portions presented wiede dispersion. For Thunnus albacares, the variation was of 0.004µg.g-1 in the liver, gills and spleen, and 0.172µg.g-1 in the muscular tissue. For Cynoscion microlepidotus, the variation was between 0.013µg.g-1 in gills and 0.250µg.g-1 in muscular tissue. The latter was the portion of higher concentration of this metal in both species, T. albacares = 0.080±0.050µg.g-1 and C. microlepidotus = 0.187±0.030µg.g-1, while less concentrations were found in the gills (0.029±0.031µg.g-1) in C. microlepidotus and in the eyes (0.013±0.008µg.g-1) in T. albacares.

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12.

Bone marrow transplantation in patients with storage diseases: a developing country experience
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Lange, Marcos C.

; Teive, Hélio A.G.

; Troiano, André R.

; Bitencourt, Marco

; Funke, Vaneuza A.M.

; Setúbal, Daniela C.

; Zanis Neto, José

; Medeiros, Carlos R.

; Werneck, Lineu C.

; Pasquini, Ricardo

; Bonfim, Carmen M.S.

Arquivos de Neuro-Psiquiatria

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mar 2006, Volume 64 N. 1 Pages 1 - 4

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O transplante de medula óssea é uma opção terapêutica para os pacientes com doenças de acúmulo. Entre 1979 e 2002, oito pacientes, quatro femininos e quatro masculinos (entre um e 13 anos de idade) foram submetidos a este procedimento em nosso centro. Seis pacientes apresentavam mucopolissacaridose (MPS I em 3; MPS III em um e MPS VI em 2), um paciente apresentava adrenoleucodistrofia e um apresentava doença de Gaucher. Cinco pacientes receberam o transplante de doador aparentado e três de doador não aparentado. Três pacientes desenvolveram doença do enxerto versus hospedeiro (dois com MPS I e um com MPS VI) e faleceram entre 37 e 151 dias após o transplante. Cinco pacientes sobreviveram entre 4 e 16 anos após o transplante. Três tiveram melhora clínica (um MPS I, um MPS VI e o paciente com doença de Gaucher), um paciente não apresentou progressão da doença (adrenoleucodistrofia) e um paciente não teve alteração da história natural da doença (MPS III).

Bone marrow transplantation (BMT) is a therapeutic option for patients with genetic storage diseases. Between 1979 and 2002, eight patients, four females and four males (1 to 13 years old) were submitted to this procedure in our center. Six patients had mucopolysaccharidosis (MPS I in 3; MPS III in one and MPS VI in 2), one had adrenoleukodystrophy (ALD) and one had Gaucher disease. Five patients had related and three unrelated BMT donor. Three patients developed graft versus host disease (two MPS I and one MPS VI) and died between 37 and 151 days after transplantation. Five patients survived 4 to 16 years after transplantation. Three patients improved (one MPS I; one MPS VI and the Gaucher disease patient), one patient had no disease progression (ALD) and in one patient this procedure did not change the natural course of the disease (MPS III).

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13.

Intoxicação por nitratos e nitritos em bovinos por ingestão de Echinochloa polystachya (capim-mandante) e Pennisetum purpureum (capim-elefante) no sertão da Paraíba
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Medeiros, Rosane M.T.

; Riet-Correa, Franklin

; Tabosa, Ivon M.

; Silva, Zoélio A.

; Barbosa, Rossemberg C.

; Marques, Ana Valéria M.S.

; Nogueira, Francisco R.B.

Pesquisa Veterinária Brasileira

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jan 2003, Volume 23 N. 1 Pages 17 - 20

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Descrevem-se três surtos de intoxicação por nitratos e nitritos em bovinos na região semi-árida do estado da Paraíba, nordeste do Brasil. O primeiro surto foi causado por Echinochloa polystachya (capim-mandante) e os demais por Pennisetum purpureum (capim-elefante) e ocorreram após um período prolongado de seca, após o início das primeiras chuvas. Em um dos surtos causado por Pennisetum purpureum, uma parte da área onde estava o pasto que continha níveis altos de nitratos havia sido fertilizada com esterco de bovino. No primeiro surto morreram 5 bovinos de um total de 11, no segundo morreram 21 de um total de 81 e no terceiro morreram 3 de um total de 19 bovinos. Os sinais clínicos se caracterizaram por anorexia, dispnéia, ranger de dentes, depressão ou hiperexitabilidade, tremores, contrações abdominais, salivação, corrimento nasal, andar cambaleante, mucosas cianóticas e, finalmente, decúbito. A presença de nitratos e nitritos foi detectada no sangue dos animais e nos pastos por meio da prova de difenilamina. Parece que o principal fator que determinou a concentração de altos níveis de nitratos nas plantas foi a ocorrência de chuvas depois de um longo período de seca. Outro fator importante no surto causado por Pennisetum purpureum foi a fertilização do solo com esterco.

Three outbreaks of nitrate poisoning are reported from the semiarid region of the state of Paraíba, northeastern Brazil. One outbreak caused by Echinochloa polystachya (capim-mandante) and two others caused by Pennisetum purpureum (capim-elefante) occurred at the end of the dry season, after the first rains. In one of the outbreaks caused by Pennisetum purpureum part of the pasture had been fertilized with cattle manure. Five out of 11 cattle died on one farm, 27 out of 81 on another, and 3 out of 9 on a third one. Clinical signs were anorexia, respiratory distress, teeth grinding, depression or hyperexitability, tremors, abdominal contractions, salivation, nasal discharge, uncoordinated gait, cyanosis, and finally recumbency. Nitrates and nitrites in the blood of affected cattle and pastures were determined by the diphenylamine test. It is suggested that the main reason for nitrate accumulation in the grasses was the prolonged draught followed by rain. In one of the outbreaks caused by Pennisetum purpureum, another reason was probably the fertilization of the soil with cattle manure.

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