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Antigen production and standardization of an in-house indirect ELISA for detection of antibodies against Anaplasma marginale
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Salinas Estrella, Elizabeth

; Ortega Hernández, María Guadalupe

; Flores Pérez, Erika

; Montenegro Cristino, Natividad

; Preciado de la Torre, Jesús Francisco

; Cobaxin Cárdenas, Mayra Elizeth

; Rodríguez, Sergio D.

Revista mexicana de ciencias pecuarias

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dec 2022, Volume 13 N. 4 Pages 1079 - 1094

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Serologic tests are important for the detection of specific antibodies against infectious agents. Commercial indirect ELISA are costly and usually as effective as in-house ELISAs. In the present work, it was prepared a batch of Anaplasma marginale crude antigen from infected blood, and tested it against official positive and negative serum controls and compared with an old batch of antigen. The new antigen batch showed an efficiency similar to the old batch. The sensitivity of the test was comparable between the new and old batches. Both, new and old antigen lots are being used at an excess. The new antigen lot is large enough to run thousands of tests at a more affordable price than commercial kits.

Resumen Las pruebas serológicas son importantes para la detección de anticuerpos específicos contra agentes infecciosos. Los ELISA indirectos comerciales son costosos y, por lo general, son tan efectivos como los ELISA caseros. En el presente trabajo fue preparado un lote de antígeno crudo de Anaplasma marginale a partir de sangre infectada, y se probó contra controles oficiales de suero positivos y negativos y comparado con un lote viejo de antígeno. El nuevo lote de antígenos mostró una eficiencia similar a la del lote viejo. La sensibilidad de la prueba entre los lotes nuevo y viejo fue comparable. Tanto el lote de antígeno nuevo como el viejo se están utilizando en exceso. El nuevo lote de antígeno es lo suficientemente grande como para ejecutar miles de pruebas a un precio más asequible que el de los kits comerciales.

https://doi.org/10.22319/rmcp.v13i4.5976

Influence of Neuropeptide Y and Neuropeptide Y 2 Receptor Variants in Acute Coronary Syndrome
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Soares, Fábia C. S.

; Araújo, Romário M.

; Werkhauser, Roberto P.

; Diniz, George T.

; Bhaskar, Lakkakula V.K.S

; Carvalho, Viviane D. C. V.

; Tashiro, Tetsuo

; Amorim, Ester A. S.

; Silva, Lilian C. A.

; Montenegro, Sergio Tavares

; Neco, Heytor V. P. C.

; Moraes, Clarice N. L.

; Martins, Danyelly B. G.

; Montenegro, Silvia M. L.

International Journal of Cardiovascular Sciences

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aug 2022, Volume 35 N. 4 Pages 444 - 456

Abstract: En | Text: En | PDF: En

Abstract Background The neuropeptide Y (NPY) is one of the most abundant neurotransmitters in the nervous system. NPY acts as a potent stimulator of angiogenesis, inflammation, and adipogenesis, through the NPY 2 receptor (NPY2R). Changes in the NPY signaling pathway have been linked to Acute Coronary Syndrome (ACS). Objectives The purpose of this study is to determine the association between variants in the NPY and NPY2R genes, as well as the severity of acute coronary syndrome (ACS). Methods Approximately 221 ACS patients and 278 healthy controls were selected for this study. Four variants in NPY and two variants in NPY2R genes were genotyped using Taqman allelic discrimination and sequencing. The Chi-square and Fisher's exact tests were used to verify the genotype frequencies. The logistic regression analyses were used for the evaluation of the studied variables. Haplotype analysis was used to evaluate the linkage disequilibrium (LD) between the variants (p<0.05). Results An association of NPY c.20T>C variant was found with the ACS group when compared to the healthy group. In the analysis between variants and risk factors in the ACS group, NPY c.84G>A was associated with hypertension. The analysis between TIMI risk showed a significance for NPY c.20T>C between the low and intermediate/high TIMI risk groups. In the haplotype analysis, strong linkage disequilibrium (LD) was found between the variants NPY c.150G>A and NPY c.-485T>C. Conclusion The NPY c.20T>C variant appears to contribute to the development of ACS. The NPY2R c.-1116A>G variant may contribute to the early development of ACS and the NPY c.84G>A variant appears to contribute to the development of hypertension. In addition, the NPY c.20T>C is associated with a protective effect in ACS severity.

https://doi.org/10.36660/ijcs.20210053

Carbon sequestration by plant species used in green roofs across different periods
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Pessoa, Victor G.

; Guiselini, Cristiane

; Montenegro, Abelardo A. de A.

; Pandorfi, Heliton

; Barbosa Filho, José A. D.

; Vicente, Thais F. da S.

Revista Brasileira de Engenharia Agrícola e Ambiental

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jun 2022, Volume 26 N. 6 Pages 407 - 411

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A presente pesquisa objetivou avaliar a quantidade de carbono estocado no substrato sob influência de espécies de plantas presentes em um telhado verde extensivo em dois períodos. A pesquisa foi desenvolvida nos meses de março e setembro de 2020, no Edifício Garagem do Empresarial Charles Darwin, Recife, Pernambuco, Brasil. Adotou-se o delineamento inteiramente casualizado em esquema de parcelas subdivididas, com as espécies de plantas (Grama Esmeralda (Zoysia japonica), Lambari roxo (Tradescantia zebrina), and Capim-do-texas (Pennisetum setaceum)) alocadas nas parcelas e os períodos de coleta dos materiais (março e setembro) nas subparcelas. Para isso, foram quantificados o teor de CO2 e concentração de carbono orgânico no substrato. Trinta amostras de substrato foram retiradas em cada período. Para o teor de CO2, não houve diferença estatística entre as espécies, apenas entre os períodos avaliados, com médias de 14.05 e 96.97 mg, para os meses de março e setembro, respectivamente. Sendo assim, setembro apresentou o maior potencial de armazenamento de CO2 no substrato. Para a concentração de carbono orgânico, houve diferença estatística entre as espécies e períodos, com valores médios de 95.05 e 129.22 g kg-1 para as espécies Capim-do-texas e Lambari roxo, respectivamente, e 131.15 e 100.81 g kg-1, para março e setembro, respectivamente.

ABSTRACT This study evaluated the influence of three plant species and two periods on the amount of carbon stored in the substrate in an extensive green roof. The study was carried out in March and September 2020 at Charles Darwin Business Garage Building, Recife, Pernambuco state, Brazil. A completely randomized design with a split-plot scheme was adopted, with plant species (zoysia grass [Zoysia japonica], inch plant [Tradescantia zebrina], and fountain grass [Pennisetum setaceum]) allocated to plots and the material collection periods (March and September) to subplots. The CO2 and organic carbon concentrations in the substrate were quantified. Thirty samples were obtained from the substrate during each period. No statistically significant difference was observed in CO2 content among species treatments; however, there was a significant difference between the two periods evaluated, with average values of 14.05 and 96.97 mg in March and September, respectively. Therefore, September has the greatest potential for CO2 storage in the substrate. Significant differences were observed in organic carbon concentrations among species and between periods, with average values of 95.05 and 129.22 g kg-1 for fountain grass and inch plant, respectively, and 131.15 and 100.81 g kg-1 in March and September, respectively.

https://doi.org/10.1590/1807-1929/agriambi.v26n6p407-411

Hematopoietic stem cell transplantation in a patient with osteopetrosis and mutation in CLCN7: long-term follow-up
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Gaytán-Morales, José F.

; Castorena-Villa, Iván

; Mendoza-Camargo, Fredenet O.

; Cortés-Flores, Dolores C.

; Gómez-Domíguez, Yazmin A.

; Montenegro-Chahar, Pamela D.

; García-Maldonado, Paulina

; Parra-Ortega, Israel

Boletín médico del Hospital Infantil de México

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jun 2021, Volume 78 N. 3 Pages 225 - 233

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Background: Osteopetrosis is a rare hereditary bone dysplasia characterized by insufficient osteoclast activity that results in increased bone mineral density. Hematopoietic stem cell transplantation (HSCT) can reverse skeletal abnormalities and restore hematopoiesis. Case report: We present the case of a 3-year and 2-month-old male patient with the diagnosis of osteopetrosis. The patient underwent allogeneic HSCT (Allo-HSCT) using 100% compatible bone marrow from a related donor and received a myeloablative conditioning regimen and a CD34 cell dose (4.7 × 107/kg). In the early post-transplant, frequent complications such as pneumonitis, hypercalcemia, and hyperphosphatemia ocurred. With a suitable granulocytic graft and chimerism of 100%, it was considered a successful transplant. However, the patient showed a delayed platelet graft treated with a platelet-stimulating factor for 6 months. The patient is currently disease-free, outpatient follow-up, with no data on graft-versus-host disease, and no progressive neurological damage. Conclusions: Osteopetrosis is a childhood disease that requires clinical suspicion and early diagnosis. HSCT is necessary at an early age to prevent disease progression and sensorineural, hematological, and endocrinological functions damage that can lead to death.

Resumen Introducción: La osteopetrosis es una displasia ósea hereditaria poco común, caracterizada por una actividad osteoclástica deficiente que aumenta la densidad mineral ósea. Se considera que el trasplante de células progenitoras hematopoyéticas (TCPH) puede revertir las anormalidades esqueléticas y restaurar la hematopoyesis. Caso clínico: Se presenta el caso de un paciente de sexo masculino, de 3 años y 2 meses de edad, con diagnóstico tardío de osteopetrosis. Se realizó un TCPH alogénico de donador relacionado 100% compatible con médula ósea. Se utilizaron un régimen de acondicionamiento mieloablativo y una dosis celular de CD34 de 4.7 × 107/kg de peso. En el postrasplante temprano, el paciente desarrolló complicaciones como neumonitis, hipercalcemia e hiperfosfatemia. Con un injerto granulocítico adecuado y quimerismo del 100% se consideró un trasplante exitoso. Sin embargo, el paciente presentó retraso en el injerto plaquetario, por lo que se administró factor estimulante de plaquetas por 6 meses. Actualmente el paciente se encuentra libre de enfermedad, en seguimiento ambulatorio, sin datos de enfermedad del injerto contra el hospedero y con pruebas de neurodesarrollo sin deterioro neurológico progresivo. Conclusiones: La osteopetrosis es una enfermedad infantil que requiere una sospecha clínica y un diagnóstico temprano, ya que es necesario un TCPH a corta edad como tratamiento para evitar la progresión de la enfermedad y el deterioro de las funciones neurosensoriales, hematológicas y endocrinológicas que puede derivar en la defunción del paciente.

https://doi.org/10.24875/bmhim.20000105 139 downloads

Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance
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Gutiérrez-Calle, Savina A.

; Sánchez-Díaz, Rosa A.

; Delgado-Silva, Yolanda B.

; Montenegro, Juan D.

; Gutiérrez, Dina L.

; Maicelo-Quintana, Jorge L.

; Guerrero-Abad, Juan C.

Scientia Agropecuaria

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jan 2021, Volume 12 N. 1 Pages 91 - 99

Abstract: En | Text: En | PDF: En

Abstract Coffee is an important agricultural commodity in the world. However, it is susceptible to Hemileia vastatrix (Hv), an obligatory biotrophic fungus that causes coffee leaf rust (CLR). Natural resistance to rust has been identified in the wild species Coffea canephora and Coffea liberica. These species have been used in breeding programs where interspecific resistant hybrids have been generated. The SH3 gene, derived from C. liberica, has been shown to confer extreme and long-lasting resistance to Hv. A total of 167 accessions of the INIA’s Coffee Germplasm Collection of Peru (INIA-CGC) were screened with 4 markers linked to the SH3 gene. As positive controls, EA67 (C. liberica) and the hybrid S.288 (C. arabica x C. liberica) were used. Separation of PCR products was done by capillary electrophoresis, which allow to discriminate the alleles of each marker. For three markers, specific alleles for either C. arabica or C. liberica species were found. In all cases, S.288 exhibited specific alleles for both species; whereas the INIA-CGC accessions had exclusively C. arabica alleles and EA67 had C. liberica alleles. The BA-48-21O-f marker did not produce PCR fragments for any of the positive controls, suggesting that this marker is not as predictive as the other three to determine the presence of SH3. This work reports the existence of multiple alleles for the Sat244 marker; however, the collection does not have the SH3 mediated-resistance gene. Finally, the utility of capillary electrophoresis as a tool to identify alleles linked to SH3 was demonstrated.

https://doi.org/10.17268/sci.agropecu.2021.011

Production and characterization of activated carbon from pineapple stubble for removal of methylene blue and surfactants
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Montenegro, D.

; Montero, N.

; Hernández, R. A.

; Méndez, J.

Journal of applied research and technology

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2021, Volume 19 N. 5 Pages 521 - 539

Abstract: En | Text: En | PDF: En

Abstract The objective of this work was to produce activated carbon from the agricultural waste known as pineapple stubble and to test its effectiveness in the removal of methylene blue and surfactants. Activated carbon was prepared using ZnCl2 and H3PO4 as activating agents and impregnation ratios of 2: 1 and 4:1 between the agent (mL) and the precursor (g) were evaluated. It was also investigated the need or not of a drying stage prior to carbonization. The produced activated carbon was characterized through FT-IR spectroscopy, X-ray fluorescence, N2 physisorption analysis, elemental analysis, and electron microscopy. Methylene blue adsorption tests were performed to determine the adsorption effectiveness of the different activated carbons produced. For such evaluation, a 23 factorial design was used, and statistical analysis determined that the activated carbon with the highest adsorption of methylene is produced under the following conditions: ZnCl2 as an activating agent, an impregnation ratio of 4:1, and with no drying step prior to carbonization. This produced activated carbon is also effective in the adsorption of the surfactant sodium dodecyl sulfate and for the surfactants present in the gray water obtained after washing clothes with commercial detergent. In these cases, removal efficiencies above 98 % were achieved. Concerning the characteristics of this activated carbon, it was observed a surface area of 685.5 m2/g, a total pore volume of 0.53 cm3/g, and an average pore diameter of 3.1 nm. Results achieved in the present study demonstrate that is possible to produce effective activated carbon for the removal of methylene blue and surfactants from agricultural waste such as pineapple stubble.

https://doi.org/10.22201/icat.24486736e.2021.19.5.1480 55 downloads

Metabolic stress and reproductive features in post-partum goats supplemented for a long period with detoxified castor meal as the source of dietary nitrogen
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Silva, L.M.

; Oliveira, C.H.A.

; Silva, A.M.

; Montenegro, A.R.

; Fernandes, C.C.L.

; Silva, C.M.G.

; Rossetto, R.

; Rondina, D.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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jan 2020, Volume 72 N. 1 Pages 136 - 144

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Este estudo avaliou o efeito da torta de mamona desintoxicada na reprodução, no estresse metabólico, na produção de leite e no desenvolvimento de cabritos no periparto de cabras. Um grupo foi alimentado com torta de mamona (DCM, n=20), e o outro (WDCM, n=21) não recebeu tal suplemento , durante a gestação até o desmame, 60 dias pós-parto. Não foram observadas diferenças significativas no período de gestação, no número de cabritos, na taxa de partos múltiplos e na mortalidade entre os dois grupos. Em todos os animais, a concentração plasmática de progesterona ficou abaixo de 1ng/mL, confirmando a ausência de atividade lútea. A espessura da gordura subcutânea do esterno e da área de olho-de-lombo, a concentração de ureia e colesterol, a produção de leite e o ganho de peso dos cabritos foram menores no grupo DCM (P<0,05). Conclui-se que o uso de torta de mamona desintoxicada no periparto de cabra resultou em cabritos mais leves devido à redução na produção de leite das matrizes e as cabras não retornaram ao cio, pois não recuperaram a massa corporal.

ABSTRACT This study evaluated the effect of detoxified castor meal on the reproductive performance, metabolic stress, milk production, and kid development in peripartum goats. The diet of the animals were with (DCM, n= 20) or without (WDCM, n= 21) detoxified castor meal during the entire gestation and until weaning, 60 days post-birth. No differences were observed in the gestation period, litter size, rate of multiple births, and mortality between the two groups. The postpartum plasma concentrations of progesterone remained below 1ng/mL in all animals, thus, confirming the absence of active corpora lutea. The thickness of sternum adipose tissue and loin area, levels of urea and cholesterol, milk production, and daily weight gain in the kids were low in the DCM group when compared to those in the WDCM group (P< 0.05). To conclude, the use of detoxified castor meal in peripartum goats resulted in lower level of performance in the kids because of reductions in the amount of milk received from their mothers during lactation. In addition, the diet containing detoxified castor meals was not efficient in recovering from the loss of stored body reserves able to initiate the recovery of the cyclic activity of the goats.

https://doi.org/10.1590/1678-4162-11210 638 downloads

Evaluación de la calidad composicional, microbiológica y sanitaria de la leche cruda en el segundo tercio de lactancia en vacas lecheras
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Jurado-Gámez, H.

; Muñoz-Domínguez, L.

; Quitiaquez-Montenegro, D.

; Fajardo-Argoti, C.

; Insuasty-Santácruz, E.

Revista de la Facultad de Medicina Veterinaria y de Zootecnia

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apr 2019, Volume 66 N. 1 Pages 53 - 66

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN Introducción: Uno de los parámetros más importantes asociados a la producción de leche y su transformación es contar con condiciones higiénico-sanitarias adecuadas que permitan mejorar las condiciones del productor y empresas transformadoras y, por ende, garantizar inocuidad alimentaria al consumidor garantizando su bienestar y salud. Objetivo: Se evaluaron características composicionales, microbiológicas y sanitarias de leche cruda durante el segundo tercio de lactancia en vacas lecheras. Materiales y métodos: Se tomaron muestras de leche por duplicado cada 10 días a siete vacas lecheras durante su segundo tercio de lactancia. Posteriormente, se realizaron las siguientes pruebas: análisis composicional, adulterantes, presencia de antibióticos, recuento de células somáticas (RCS/ ml), identificación de Staphylococcus aureus y antibiograma por el método de Kirby-Bauer. Resultados: Se detectó la presencia de Staphylococcus aureus en el 66,2% de las muestras y 4,6% resultaron positivas para Listeria sp. No hubo presencia de antibióticos, peróxidos y almidones. Se evidenciaron muestras positivas para cloruros y neutralizantes, además se determinó que por cada kilogramo de leche se obtiene 30, 25 y 84 g de proteína, caseína y sólidos no grasos (SNG), respectivamente; donde la caseína representó el 83% de la proteína. Para el caso del RCS/ml se encontraron valores superiores a un millón de RCS/ml. Conclusiones: Para el caso estudiado la calidad composicional, sanitaria y microbiológica de la leche cruda en el segundo tercio de lactancia en vacas lecheras no es adecuada debido a que se obtuvieron valores que sobrepasan algunos lineamientos de la Norma Técnica Colombiana (NTC) 399 de 2002, en la cual se establece un conteo de mesófilos y de células somáticas (RCS/ml) máximo de 700.000/ml de leche cruda, a partir del cual se debe descartar el consumo de leche o su transformación.

ABSTRACT Introduction: One of the most important parameters associated with the production of milk and its transformation, is to have adequate hygienic-sanitary conditions in terms of milk quality that allow improving the conditions of the producer and processing companies, and therefore to provide food safety to the consumers, guaranteeing their well-being and health. Objetive: Compositional, microbiological and sanitary characteristics of raw milk were evaluated during the second third of lactation in dairy cows. Materials and methods: Two milk samples were taken every 10 days from 7 dairy cows during their second third of lactation. Then the following tests were carried out: compositional analysis, adulterants, presence of antibiotics, somatic cell count (SCC/mL), identification of Staphylococcus aureus and antibiogram by the Kirby-Bauer method. Results: Staphylococcus aureus was evidenced in 66.2% of the samples, and 4.6% of them gave positive for Listeria sp. No traces of antibiotics, peroxides and starches were found. Positive samples were found for chlorides and neutralizers, and it was determined that for each kilogram of milk, 30, 25 and 84 grams of protein, casein and non-fatty solids (NFS) were obtained, with 83% of the protein formed by casein. In the case of SCC/mL, values higher than one million SCC/mL were found. Conclusions: The compositional, sanitary and microbiological quality of the studied raw milk in the second third of lactation in dairy cows was not adequate, because values found lied above the Colombian technical standard 399 of 2002, which establishes a maximum of mesophile and somatic cell count (SCC/ mL) of 700000/ml in raw milk, therefore not allowing its consumption and transformation.

https://doi.org/10.15446/rfmvz.v66n1.79402 1620 downloads

Tunable linear fiber laser cavity based on a twisted mechanical long period grating
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Huerta-Mascotte, E.

; Estudillo-Ayala, J. M.

; Mata-Chávez, R.I.

; Jáuregui-Vázquez, D.

; Sierra-Hernández, J.M.

; Montenegro-Orenday, J.A.

; Estrada-García, H.J.

; Rojas-Laguna, R.

Revista mexicana de física

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dec 2018, Volume 64 N. 6 Pages 615 - 618

Abstract: En | Text: En | PDF: En

Abstract The present manuscript shows linear cavity fiber laser experimental analysis. The fiber laser arrangement, uses an all fiber mechanical long period grating (MLPG) to control single and dual laser emission. Here, single laser emission centered at 1562 nm with a signal to noise ratio of 27 dB and a linewidth less than 0.1 nm is obtained and tuned from 1562 nm to 1546 nm, by twisting a conventional section of single mode fiber that was set into the MLPG. Thus, when the twist is around 120∘ dual emission is obtained with centered wavelengths at 1533 nm and 1546 nm, these emissions can be switched between them when the twist is incremented and the peak centered at 1546 nm is tuned in backward direction. The proposed laser offers wavelength and power stability with minimal variations of 0.5 dB and 0.5 nm respectively. This laser can be applied in several fields such as sensing, optical signal analysis and, optical communications.

https://doi.org/10.31349/revmexfis.64.615 211 downloads

10.

Anterior fixation of odontoid fractures: results
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Lobo, João Pedro Ferraz Montenegro

; Moutinho, Vitorino Veludo

; Serdoura, António Francisco Martingo

; Oliveira, Carolina Fernandes

; Pinho, André Rodrigues

Revista Brasileira de Ortopedia

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oct 2018, Volume 53 N. 5 Pages 532 - 536

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: Avaliar os resultados clínicos e radiológicos do tratamento cirúrgico em pacientes com diagnóstico de fratura do processo odontoide submetidos a redução aberta e fixação interna (RAFI) com parafusos. Métodos: Estudo retrospectivo com nove pacientes. Avaliada a dor (escala visual analógica [EVA]) e o estado neurológico (escala de Frankel). O Neck Disability Index (NDI) e a amplitude de movimento cervical pós-operatória foram calculados. A coluna cervical foi avaliada radiologicamente (raios X e TC) nos períodos pré- e pós-operatório. Resultados: A idade média dos pacientes foi de 70 anos. Todos apresentaram fraturas do tipo IIb (classificação de Grauer), com desvio médio de 2,95 mm. Dois apresentaram lesões subaxiais. O seguimento médio foi de 30 meses. O tempo médio entre trauma e cirurgia foi de sete dias. O escore pré-operatório de Frankel foi E em todos, exceto em um paciente (B), no qual se observou uma melhoria pós-operatória de B para D. A dor pós-operatória foi 2/10 (EVA). Apresentaram incapacidade leve ou moderada (NDI) 77% pacientes. Seis pacientes recuperaram toda a amplitude de movimento cervical; a consolidação óssea levou aproximadamente 14 semanas. Foram observadas complicações de pseudartrose em dois pacientes (taxa de consolidação: 77%), um paciente necessitou reposicionamento do parafuso e um paciente, disfonia. Conclusão: O diagnóstico tardio ainda é um problema no tratamento de fraturas do odontoide, especialmente em pacientes idosos. As lesões concomitantes, especialmente em pacientes mais jovens, não são incomuns. A literatura apresenta altas taxas de consolidação com RAFI (≥ 80%), o que também foi observado no presente estudo. No entanto, o sucesso cirúrgico depende da seleção adequada do paciente e do conhecimento rigoroso da técnica. Essa patologia apresenta um prognóstico funcional reservado em médio prazo, especialmente em idosos.

ABSTRACT Objective: To evaluate the clinical and radiological outcomes of the surgical treatment in patients diagnosed with odontoid fracture who underwent open reduction and internal fixation (ORIF) with screws. Methods: This was a retrospective study with nine patients. Pain (visual analog scale [VAS]) and neurological status (Frankel scale) were assessed. The neck disability index (NDI) and the post-operative cervical range of motion were calculated. The cervical spine was radiologically evaluated (X-ray and CT) pre- and postoperatively. Results: The mean age of patients was 70 years. All patients presented type IIb (Grauer classification) fractures, with a mean deviation of 2.95 mm. Two patients had subaxial lesions. The mean follow-up was 30 months. The mean time from trauma to surgery was seven days. The pre-operative Frankel score was E in all except one patient (B), in whom a post-operative improvement from B to D was observed. Post-operative pain was 2/10 (VAS). A total of 77% of patients presented a mild or moderate disability (NDI). Six patients regained full range of cervical movement, and bone union required approximately 14 weeks. Pseudarthrosis complications were observed in two patients (77% union rate), one patient presented screw repositioning and one case, dysphonia. Conclusion: Delayed diagnosis is still an issue in the treatment of odontoid fractures, especially in elderly patients. Concomitant lesions, especially in younger patients, are not uncommon. The literature presents high fusion rates with ORIF (≥80%), which was also observed in the present study. However, surgical success depends on proper patient selection and strict knowledge of the technique. This pathology presents a reserved functional prognosis in the medium-term, especially in the elderly.

https://doi.org/10.1016/j.rboe.2017.07.010 2056 downloads

11.

New flavone and other compounds from Tephrosia egregia: assessing the cytotoxic effect on human tumor cell lines
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Teixeira, Maria V.S.

; Lima, Jefferson Q.

; Pimenta, Antônia T.A.

; Silva, Francisca R.L. da

; Oliveira, Maria da Conceição F. de

; Pereira, Italo G.

; Costa-Junior, Dilailson C.

; Ribeiro, Paulo R.V.

; Santiago, Gilvandete M.P.

; Lima, Mary Anne S.

; Braz-Filho, Raimundo

; Bezerra, Mirna M.

; Montenegro, Raquel C.

; Rocha, Danilo D.

; Moraes, Maria Elisabete A. de

; Medeiros, Aline C. de

; Arriaga, Angela M.C.

Revista Brasileira de Farmacognosia

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jun 2018, Volume 28 N. 3 Pages 333 - 338

Abstract: En | Text: En | PDF: En

ABSTRACT The organic extracts from stems, roots and leaves of Tephrosia egregia Sandwith, Fabaceae, provided a new flavone, 5-hydroxy-8-(1",2"-epoxy-3"-hydroxy-3"-methylbutyl)-7-methoxyflavone (1), in addition to eleven known compounds: pongaflavone (2), praecansone B (3), 12a-hydroxyrotenone (4), praecansone A, 2',6'-dimethoxy-4',5'-(2",2"-dimethyl)-pyranochalcone, pongachalcone, maackiain, β-sistosterol and its glucoside, p-cumaric acid and cinnamic acid. The structures of all compounds were established on the basis of spectroscopic methods, mainly 1D and 2D NMR and HRESIMS, involving comparison with literature data. Cytotoxicity of compounds 1–4 was evaluated against AGP-01 (cancerous ascitic fluid), HCT-116 (colon adenocarcinoma), HL-60 (leukemia), PC-3 (prostate carcinoma), SF-295 (glioblastoma) and SKMEL 28 (melanoma) cell lines.

https://doi.org/10.1016/j.bjp.2018.03.008 525 downloads

12.

Controversial issues in the management of hyperprolactinemia and prolactinomas – An overview by the Neuroendocrinology Department of the Brazilian Society of Endocrinology and Metabolism
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Vilar, Lucio

; Abucham, Julio

; Albuquerque, José Luciano

; Araujo, Luiz Antônio

; Azevedo, Monalisa F.

; Boguszewski, Cesar Luiz

; Casulari, Luiz Augusto

; Cunha Neto, Malebranche B. C.

; Czepielewski, Mauro A.

; Duarte, Felipe H. G.

; Faria, Manuel dos S.

; Gadelha, Monica R.

; Garmes, Heraldo M.

; Glezer, Andrea

; Gurgel, Maria Helane

; Jallad, Raquel S.

; Martins, Manoel

; Miranda, Paulo A. C.

; Montenegro, Renan M.

; Musolino, Nina R. C.

; Naves, Luciana A.

; Ribeiro-Oliveira Júnior, Antônio

; Silva, Cíntia M. S.

; Viecceli, Camila

; Bronstein, Marcello D.

Archives of Endocrinology and Metabolism

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mar 2018, Volume 62 N. 2 Pages 236 - 263

Abstract: En | Text: En | PDF: En

ABSTRACT Prolactinomas are the most common pituitary adenomas (approximately 40% of cases), and they represent an important cause of hypogonadism and infertility in both sexes. The magnitude of prolactin (PRL) elevation can be useful in determining the etiology of hyperprolactinemia. Indeed, PRL levels > 250 ng/mL are highly suggestive of the presence of a prolactinoma. In contrast, most patients with stalk dysfunction, drug-induced hyperprolactinemia or systemic diseases present with PRL levels < 100 ng/mL. However, exceptions to these rules are not rare. On the other hand, among patients with macroprolactinomas (MACs), artificially low PRL levels may result from the so-called “hook effect”. Patients harboring cystic MACs may also present with a mild PRL elevation. The screening for macroprolactin is mostly indicated for asymptomatic patients and those with apparent idiopathic hyperprolactinemia. Dopamine agonists (DAs) are the treatment of choice for prolactinomas, particularly cabergoline, which is more effective and better tolerated than bromocriptine. After 2 years of successful treatment, DA withdrawal should be considered in all cases of microprolactinomas and in selected cases of MACs. In this publication, the goal of the Neuroendocrinology Department of the Brazilian Society of Endocrinology and Metabolism (SBEM) is to provide a review of the diagnosis and treatment of hyperprolactinemia and prolactinomas, emphasizing controversial issues regarding these topics. This review is based on data published in the literature and the authors' experience.

https://doi.org/10.20945/2359-3997000000032 36158 downloads

13.

Challenges for conducting blood collection and biochemical analysis in a large multicenter school-based study with adolescents: lessons from ERICA in Brazil
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Cureau, Felipe Vogt

; Bloch, Katia Vergetti

; Henz, Aline

; Schaan, Camila W.

; Klein, Carlos Henrique

; Oliveira, Cecília Lacroix de

; Giannini, Denise Tavares

; Leon, Elisa Brosina de

; Abreu, Gabriela de Azevedo

; Telo, Gabriela Heiden

; Dias, Glauber Monteiro

; Carvalho, Kênia Mara Baiocchi de

; Barufaldi, Laura Augusta

; Kuschnir, Maria Cristina Caetano

; Szklo, Moyses

; Montenegro Jr., Renan

; Silva, Thiago Luiz Nogueira da

; Ekelund, Ulf

; Schaan, Beatriz D.

Cadernos de Saúde Pública

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2017, Volume 33 N. 4 elocation e00122816

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Resumo: O Estudo de Riscos de Cardiovasculares em Adolescentes (ERICA) é um estudo pioneiro que tem como objetivo avaliar a prevalência de fatores de risco cardiovascular, incluindo componentes da síndrome metabólica, entre adolescentes brasileiros. Este artigo tem como objetivo descrever os aspectos metodológicos relacionados com a coleta de sangue, assim como informar os resultados da preparação, transporte, armazenamento e exames no ERICA. Os exames foram realizados em um único laboratório e as amostras de sangue foram coletadas de forma padronizada. A logística envolveu o transporte aéreo das amostra até o laboratório de referência, com a temperatura controlada desde a coleta do sangue. O soro foi armazenado em biorrepositores locais em quatro centros e se serão utilizados em análises futuras. Durante o estudo foram realizados 284.247 exames e a taxa de participação foi de 56,2%, representando 40.732 adolescentes. Do total, 92,6% das amostras chegaram ao laboratório de referência mantendo a temperatura entre 0-10°C. Não foram identificadas alterações clínicas significativas nos resultados devido a mudanças de temperatura. O controle de qualidade externo registrou resultados satisfatórios em 98,7% das avaliações. Foram criados quatro biorrepositores com amostras de 7.785 adolescentes. Assim, podemos considerar que a logística adotada no ERICA foi bastante exitosa e sua descrição, tal como as dificuldades experimentadas no Brasil, podem informar e facilitar o planejamento de futuros estudos, especialmente nos países em desenvolvimento.

Abstract: The Study of Cardiovascular Risk in Adolescents (ERICA) is a pioneering study that aimed to assess the prevalence of cardiovascular risk factors, including metabolic syndrome components in Brazilian adolescents. This study aims to describe the methodological aspects related to blood collection as well as to report pertaining results of the preparation, transport, storage, and exams in ERICA. Exams in ERICA were performed in a single laboratory and blood samples were collected in schools in a standardized manner. Logistics involved air transportation of samples to the reference laboratory with controlled temperature since sample collection. The serum was stored in local biorepositories in four centers to be used in future analyses. During the study, 284,247 exams were performed and rate of participation in exams was 56.2%, thus involving 40,732 adolescents. From the total, 92.6% of the samples reached the reference laboratory maintaining the temperature between 0-10°C. No clinical significant changes in results due to temperature changes were identified. External quality control recorded satisfactory results in 98.7% of the evaluations. Four biorepositories with samples of 7,785 adolescents were created. Thus, we can consider that the logistics adopted in ERICA was fairly successful and description of this as well as the difficulties experienced in Brazil can inform and facilitate the planning of future studies, especially in developing countries.

Resumen: El Estudio de Riesgos Cardiovasculares en Adolescentes (ERICA) es un estudio pionero que tiene como objetivo evaluar la prevalencia de factores de riesgo cardiovascular, incluyendo componentes del síndrome metabólico en adolescentes brasileños. Este estudio tiene como objetivo describir los aspectos metodológicos relacionados con la recolección de sangre, así como informar sobre los resultados de la preparación, transporte, almacenamiento y exámenes en el ERICA. Los exámenes en ERICA se realizaron en un solo laboratorio y se recogieron muestras de sangre en las escuelas de manera estandarizada. La logística involucró el transporte aéreo de muestras al laboratorio de referencia con temperatura controlada desde la recolección de muestras. El suero fue almacenado en biorepositories locales en cuatro centros que se utilizarán en análises futuros. Durante el estudio se realizaron 284.247 exámenes y la tasa de participación fue de 56,2%, lo que involucró a 40.732 adolescentes. Del total, el 92,6% de las muestras alcanzaron el laboratorio de referencia manteniendo la temperatura entre 0-10°C. No se identificaron cambios clínicos significativos en los resultados debido a cambios de temperatura. El control de calidad externo registró resultados satisfactorios en el 98,7% de las evaluaciones. Se crearon cuatro biorrepositores con muestras de 7.785 adolescentes. Así, podemos considerar que la logística adoptada en el ERICA fue bastante exitosa y su descripción así como las dificultades experimentadas en Brasil pueden informar y facilitar la planificación de futuros estudios, especialmente en los países en desarrollo.

https://doi.org/10.1590/0102-311x00122816 365 downloads

14.

Challenges for conducting blood collection and biochemical analysis in a large multicenter school-based study with adolescents: lessons from ERICA in Brazil
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Cureau, Felipe Vogt

; Bloch, Katia Vergetti

; Henz, Aline

; Schaan, Camila W.

; Klein, Carlos Henrique

; Oliveira, Cecília Lacroix de

; Giannini, Denise Tavares

; Leon, Elisa Brosina de

; Abreu, Gabriela de Azevedo

; Telo, Gabriela Heiden

; Dias, Glauber Monteiro

; Carvalho, Kênia Mara Baiocchi de

; Barufaldi, Laura Augusta

; Kuschnir, Maria Cristina Caetano

; Szklo, Moyses

; Montenegro Jr., Renan

; Silva, Thiago Luiz Nogueira da

; Ekelund, Ulf

; Schaan, Beatriz D.

Cadernos de Saúde Pública

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2017, Volume 33 N. 4 elocation e00122816

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

https://doi.org/10.1590/0102-311x00122816 2977 downloads

15.

Síndrome general y dolores óseos en un paciente tratado con tenofovir
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Arnaiz de las Revillas, F.

; Marcote Ibáñez, C.

; González-Lamuño, D.

; García Unzueta, M.

; Lino Montenegro, E.

; Riancho, J.A.

Revista de Osteoporosis y Metabolismo Mineral

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dec 2015, Volume 7 N. 4 Pages 112 - 114

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Tenofovir (TDF), is the only nucleotide analogue reverse transcriptase inhibitor for treating human immunodeficiency virus (HIV). Occasionally, it may cause acute renal failure and Fanconi syndrome. We report the case of a 64-year-old male diagnosed with HIV infection 22 years previous and treated with tenofovir. In outpatient follow-up, the patient complained of progressive fatigue and diffuse aching bones. In several check-ups, increased alkaline phosphatase and parathyroid hormone (PTH) were observed. Over the past month, his condition worsened and he was admitted to hospital. Analytical data included marked glycosuria, hypophosphatemia, hyperphosphaturia and hypouricemia. All changes were resolved when TDF was discontinued.This illustrates the importance of clinical evaluations that include possible TDF-induced proximal tubulopathy in patients with general bone pain syndrome or mineral metabolism disturbances.

El tenofovir (TDF), es el único inhibidor de la transcriptasa inversa análogo nucleótido para el tratamiento de la infección por virus de la inmunodeficiencia humana (VIH). Ocasionalmente, puede producir insuficiencia renal aguda y síndrome de Fanconi. Presentamos el caso de un varón de 64 años con infección por VIH conocida desde hace 22 años, en tratamiento con tenofovir. En las revisiones ambulatorias refería un cuadro progresivo de astenia y dolores óseos difusos. En varias determinaciones se había observado una elevación de la fosfatasa alcalina y la paratohormona (PTH). Durante el último mes empeoró su estado, por lo que fue ingresado en el hospital. Entre los datos analíticos destacaban: glucosuria marcada, hipofosfatemia, hiperfosfaturia e hipouricemia. Todas las alteraciones se resolvieron tras suspender el TDF, lo que ilustra la importancia de que los clínicos incluyan la posibilidad de tubulopatía proximal por TDF en pacientes con dolores óseos, síndrome general o alteraciones del metabolismo mineral.

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