Abstract INTRODUCTION: Aedes aegypti is the main vector of dengue and yellow fever. Recently, the use of plant-sourced larvicides has gained momentum. METHODS: The hydroethanolic extracts and fractions ofOcotea nutansleaves and stems were bioassayed to determine the larvicidal efficacy of these samples. RESULTS: S-HEX (hexane fraction from the crude stem extract) demonstrated high potential for controlling third-stage larvae, with an LC50 of 14.14 µg.mL-1 (concentration required to inhibit 50% of the treated larvae). CONCLUSIONS Extracts from O. nutans were effective against third-stage larvae ofA. aegyptiafter 24 h of exposure.

The article has aimed to characterize the essential oil extracted from Ocotea diospyrifolia (Meisn.) Mez. leaves, in terms of its chemical composition and antioxidant, hemolytic, and phytotoxic potentials, as well as its toxicity against Artemia salina. The major constituents identified by CG-MS were d-elemene, spathulenol, and b-atlantol. When screened for potential biological activities, the essential oil presented low toxicity against Artemia salina, and a capacity of lysing red blood cells. Also, the evaluation of its in vitro antioxidant activity, using the phosphomolybdenum method, showed better results when compared to butylhydroxytoluene (BHT) and rutin. In conclusion, the results obtained showed certain in vitro toxicity, leading to an interesting target for cytotoxicity evaluations of carcinoma cells.

This work describes the occurrence and contents of aporphinoids alkaloids in seedlings of Ocotea puberula from germination until 12 months old and in leaves from adult plants. Seedling leaves showed an alkaloids profile similar to leaves of adult plant. However, leaves in seedlings showed higher contents of the alkaloids boldine, dicentrine, leucoxine and isodomesticine when compared to adult plants. The alkaloids concentration in stems and leaves increased during the development of the seedlings, followed by a remarkable decrease of these compounds in roots. Cultivation in a seedling-nursery method is also described.

A Atenção Farmacêutica, entendida como um modelo de prática profissional desenvolvida no contexto da assistência farmacêutica, de acordo com a proposta de Consenso Brasileiro de Atenção Farmacêutica, possui por finalidade aumentar a efetividade do tratamento medicamentoso, concomitante à detecção de problemas relacionados a medicamentos (PRMs). Compõe uma prática que vem sendo gradualmente aplicada em número crescente de farmácias comunitárias em diversas regiões, porém ainda como projetos individuais, enfrentando diversas dificuldades na sua incorporação, em parte devido ao desconhecimento e despreparo dos profissionais, bem como por certa rejeição por parte de proprietários de farmácias. Nesse contexto, realizou-se uma pesquisa com característica participativa, por meio de entrevista com questões abertas a farmacêuticos e proprietários de farmácia no Município de Curitiba, na qual houve interação das idéias, com o propósito de estabelecer permuta de informações sobre o referido conhecimento. Os resultados obtidos confirmam que a implantação da Atenção Farmacêutica enfrenta obstáculos que incluem o vínculo empregatício do profissional farmacêutico e a rejeição do programa por gerentes e proprietários das farmácias, além da insegurança e desmotivação por parte dos farmacêuticos, decorrente de elevada carga labutária e falta de tempo para dedicar-se ao atendimento, além da concorrência dos balconistas em busca de comissões sobre vendas. Constata-se a necessidade de estimular a atuação profissional, principalmente de acadêmicos e egressos profissionais, o que pode representar um primeiro passo ao sucesso da Atenção Farmacêutica e à abertura por parte dos empresários, uma vez que a sociedade começa a reconhecer a importância do atendimento realizado pelo farmacêutico.

Pharmaceutical care, a professional practice in the context of pharmaceutical assistance, has the goal of increasing the efficiency of medical treatment, as well as the detection of medicine-related problems. It consists in a practice that has being gradually applied in pharmacies of several regions of Brazil, and faces several difficulties for this, in part due to the lack of knowledge and preparation of our professionals, as well as certain rejection from the owners of the pharmacies. In this context, a research with participative characteristic was carried out, by means of interview with open questions to pharmacists and pharmacy owners and/or managers of Curitiba city, in which there were interaction of ideas, in order to establish an exchange of information about the subject. The results confirmed that the implantation of Pharmaceutical Care faces obstacles that include the link of the professional to the pharmacy and the rejection of the program by managers and owners of pharmacies, due to the high content of daily work and lack of time to dedicate to the service, besides the competition of sellers that seek commission on sales. It's observed the need of a way to stimulate the professional actuation, mainly among students and recently graduated professionals. This can represent the first step towards the success of Pharmaceutical Care and opening from the entrepreneurs, once the population starts to recognize the importance of the work provided by the pharmacist.

A new extraction method for sterols was tested in fruits of Ottonia martiana Miq., a shrub belonging to the family Piperaceae, popularly known as "anestésica" due to the use of its roots and aerial parts, in alcoholic preparations, in treatment of odontological problems. For this purpose, a portable extraction equipment was developed, to operate with liquified gases, which showed to be practical, fast and effective, besides being economical and ecological. In the chromatographic analysis of the extracts obtained in this equipment, it was demonstrated the presence of two sterols, isomers of b-sitosterol and stigmasterol.