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Association mapping for common bacterial blight in carioca beans
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Barbosa, Caio Cesar Ferrari

; Paulino, Jean Fausto Carvalho

; Almeida, Caléo Panhoca

; Carbonell, Sérgio Augusto Morais

; Chiorato, Alisson Fernando

; Benchimol-Reis, Luciana Lasry

Crop Breeding and Applied Biotechnology

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2022, Volume 22 N. 3 elocation e41712239

Abstract: En | Text: En | PDF: En

Abstract Common bacterial blight (CBB) is an important disease in Phaseolus vulgaris L. A carioca diversity panel (CDP) composed of 149 cultivars of common bean was genotyped with 1,616 SNPs and evaluated for Xanthomonas phaseoli pv. phaseoli (Xap) resistance aiming to identify QTLs. Phenotypic evaluation was done in controlled conditions. The plants displayed in a randomized block design, with 3 replications were evaluated ten days after inoculation. GWAS analysis using the BLINK model identified one SNP on chromosome Pv07, with a different genomic location in relation to previous studies. Considering a confidence interval of 100 kb, gene annotation identified 13 candidate genes related to defense-associated genes, and their key functional motives were discussed. The marker identified may constitute an important resource for future marker assisted CBB resistance breeding.

https://doi.org/10.1590/1984-70332022v22n3a32

Performance Diagnóstica da FFR por Angiotomografia de Coronárias através de Software Baseado em Inteligência Artificial
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Morais, Thamara Carvalho

; Assunção-Jr, Antonildes Nascimento

; Dantas Júnior, Roberto Nery

; Silva, Carla Franco Grego da

; Paula, Caroline Bastida de

; Torres, Roberto Almeida

; Magalhães, Tiago Augusto

; Nomura, César Higa

; Ávila, Luiz Francisco Rodrigues de

; Parga Filho, José Rodrigues

Arquivos Brasileiros de Cardiologia

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jun 2021, Volume 116 N. 6 Pages 1091 - 1098

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Fundamento A quantificação não invasiva da reserva fracionada de fluxo miocárdico (FFR TC ) através de software baseado em inteligência artificial em versão mais atualizada e tomógrafo de última geração (384 cortes) apresenta elevada performance na detecção de isquemia coronariana. Objetivos Avaliar o desempenho diagnóstico da FFR TC na detecção de doença arterial coronariana (DAC) significativa em relação ao FFRi, em tomógrafos de gerações anteriores (128 e 256 cortes). Métodos Estudo retrospectivo com pacientes encaminhados à angiotomografia de artérias coronárias (TCC) e cateterismo (FFRi). Foram utilizados os tomógrafos Siemens Somatom Definition Flash (256 cortes) e AS+ (128 cortes). A FFR TC e a área luminal mínima (ALM) foram avaliadas em software (cFFR versão 3.0.0, Siemens Healthineers, Forchheim, Alemanha). DAC obstrutiva foi definida como TCC com redução luminal ≥50% e DAC funcionalmente obstrutiva como FFRi ≤0,8. Todos os valores de p reportados são bicaudais; e quando <0,05, foram considerados estatisticamente significativos. Resultados Noventa e três pacientes consecutivos (152 vasos) foram incluídos. Houve boa concordância entre FFR TC e FFRi, com mínima superestimação da FFR TC (viés: –0,02; limites de concordância: 0,14 a 0,09). Diferentes tomógrafos não modificaram a relação entre FFR TC e FFRi (p para interação = 0,73). A FFR TC demonstrou performance significativamente superior à classificação visual de estenose coronariana (AUC 0,93 vs. 0,61, p <0,001) e à ALM (AUC 0,93 vs. 0,75, p <0,001) reduzindo o número de casos falso-positivos. O melhor ponto de corte para a FFR TC utilizando um índice de Youden foi de 0,85 (sensiblidade, 87%; especificidade, 86%; VPP, 73%; NPV, 94%), com redução de falso-positivos. Conclusão FFR TC baseada em inteligência artificial, em tomógrafos de gerações anteriores (128 e 256 cortes), apresenta boa performance diagnóstica na detecção de DAC, podendo ser utilizada para reduzir procedimentos invasivos.

Abstract Background The non-invasive quantification of the fractional flow reserve (FFRCT) using a more recent version of an artificial intelligence-based software and latest generation CT scanner (384 slices) may show high performance to detect coronary ischemia. Objectives To evaluate the diagnostic performance of FFRCT for the detection of significant coronary artery disease (CAD) in contrast to invasive FFR (iFFR) using previous generation CT scanners (128 and 256- detector rows). Methods Retrospective study with patients referred to coronary artery CT angiography (CTA) and catheterization (iFFR) procedures. Siemens Somatom Definition Flash (256-detector rows) and AS+ (128-detector rows) CT scanners were used to acquire the images. The FFRCT and the minimal lumen area (MLA) were evaluated using a dedicated software (cFFR version 3.0.0, Siemens Healthineers, Forchheim, Germany). Obstructive CAD was defined as CTA lumen reduction ≥ 50%, and flow-limiting stenosis as iFFR ≤0.8. All reported P values are two-tailed, and when <0.05, they were considered statistically significant. Results Ninety-three consecutive patients (152 vessels) were included. There was good agreement between FFRCT and iFFR, with minimal FFRCT overestimation (bias: -0.02; limits of agreement:0.14-0.09). Different CT scanners did not modify the association between FFRCT and FFRi (p for interaction=0.73). The performance of FFRCT was significantly superior compared to the visual classification of coronary stenosis (AUC 0.93vs.0.61, p<0.001) and to MLA (AUC 0.93vs.0.75, p<0.001), reducing the number of false-positive cases. The optimal cut-off point for FFRCT using a Youden index was 0.85 (87% Sensitivity, 86% Specificity, 73% PPV, 94% NPV), with a reduction of false-positives. Conclusion Machine learning-based FFRCT using previous generation CT scanners (128 and 256-detector rows) shows good diagnostic performance for the detection of CAD, and can be used to reduce the number of invasive procedures.

https://doi.org/10.36660/abc.20190329 391 downloads

EFFECT OF ARTIFICIAL WEATHERING ON PHYSICAL AND MECHANICAL PROPERTIES OF WOOD
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Aquino, Vinicius Borges de Moura

; Bertolini, Marilia Silva

; Morais, Cesar Augusto Galvão de

; Almeida, Tiago Hendrigo

; Almeida, Diego Henrique

; Lahr, Francisco Antonio Rocco

; Christoforo, André Luis

Revista Árvore

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2021, Volume 45 elocation e4534

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Quando a madeira é exposta ao ar livre, uma combinação de fatores químicos e mecânicos e a radiação solar contribuem para o que se denomina intemperismo, sendo o principal agente de degradação nesse ambiente. Este trabalho tem como objetivo investigar o efeito do intemperismo artificial nas propriedades mecânicas e físicas de Eucalyptus sp. e de Cupiúba (Goupia glabra) simulando efeitos naturais do intemperismo. As amostras foram envelhecidas em câmara de radiação UV com controle de umidade e temperatura por 100, 200, 300 e 400 horas, considerando os ciclos de envelhecimento de acordo com ASTM G154 (2006). As propriedades da madeira investigadas foram valor convencional de resistência à flexão estática (fM), módulo de elasticidade à flexão estática (EM), resistência à compressão paralela à fibra (fc0) e dureza Janka (fH) de acordo com a ABNT NBT 7190 (1997). Os efeitos do intemperismo artificial nas propriedades da madeira foram avaliados por análise estatística com nível de significância de 5%. A maioria das propriedades da madeira investigadas não apresentou alterações significativas com o envelhecimento realizado, porém, observou-se diminuição dos valores absolutos das propriedades da madeira durante o processo de envelhecimento. Apenas o fH da madeira de Cupiúba envelhecida por 100 e 200 horas apresentou perda significativa de desempenho no nível de significância considerado, o que pode estar relacionado a alterações na superfície da madeira devido à exposição ao intemperismo.

ABSTRACT When wood is exposed outdoors, a combination of chemical and mechanical factors and solar radiation contribute to what is described as weathering, being the main degradation agent in this environment. This paper aims to investigate the effect of artificial weathering on mechanical and physical properties of Eucalyptus sp. and Cupiúba (Goupia glabra) woods simulating natural weathering effects. Samples were aged in UV radiation chamber with humidity and temperature control for 100, 200, 300 and 400 hours, considering aging cycles according to ASTM G154 (2006). Wood properties investigated were Conventional value of strength in static bending (fM), Modulus of elasticity in static bending (EM), strength in compression parallel to grain (fc0) and Janka Hardness (fH) according to ABNT NBT 7190 (1997). Effects of artificial weathering on wood properties were evaluated by statistical analysis at 5% significance level. Most of the wood properties investigated did not present significant changes with the aging performed, however, it was noted a decrease in the absolute values of the wood properties absolute values during the aging process. Only fH of Cupiúba wood aged for 100 and 200 hours presented significative performance loss at the significance level considered, which can be related to changes on the wood surface due to weathering exposure.

https://doi.org/10.1590/1806-908820210000034 102 downloads

Residues of glyphosate and aminomethylphosphonic acid (AMPA) in genetically modified glyphosate tolerant soybean, corn and cotton crops
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Rodrigues, Nadia Regina

; Souza, Ana Paula Ferreira de

; Morais, Pedro Patric Pinho

; Braga, Daniella Pascon Vianna

; Crivellari, Augusto Cesar

; Favoretto, Luís Roberto Graça

; Berger, Geraldo Ubirajara

Ciência Rural

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2021, Volume 51 N. 1 elocation e20190244

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O glifosato é um herbicida de amplo espectro capaz de controlar uma grande diversidade de ervas daninhas e seu uso pode acarretar na ocorrência de resíduos, seja em culturas convencionais ou geneticamente modificadas. Sendo assim, amostragens de grãos de soja e milho e de sementes de algodão foram realizadas em áreas comerciais nas safras de 2012/2013 a 2017/2018 em diferentes regiões agrícolas brasileiras com o objetivo de monitorar os níveis de resíduos de glifosato e seu metabólito (ácido aminometilfosfônico - AMPA) após diferentes manejos. Os níveis de resíduos de glifosato em milho geneticamente modificados (GM) tolerante ao glifosato variaram desde não detectados (ND) a até 0,15 mg kg-1, em soja GM tolerante ao glifosato os valores variaram de ND a 2,81 mg kg-1 e em algodão GM tolerante ao glifosato os resultados se estabeleceram entre ND a 1,78 mg kg-1. Os valores de resíduos de AMPA indicaram correlação com os resíduos de glifosato. Os níveis de resíduos de glifosato em grãos de soja e milho e sementes de algodão ficaram dentro dos Limites Máximos de Resíduos (LMRs) preconizados pela ANVISA e Codex Alimentarius.

ABSTRACT: Glyphosate is a broad-spectrum herbicide capable of controlling a wide range of weed. It uses could cause the occurrence of residues in conventional or genetically modified crops. For this purpose, grains (soybean and corn) and cotton seeds were sampled from commercial areas in 2012/2013 to 2017/2018 seasons in different Brazilian agricultural regions to monitor glyphosate residues levels and its metabolite (aminomethylphosphonic acid - AMPA) after different managements. The glyphosate residues levels in genetically modified corn (GM) ranged from no detected (ND) to 0.15 mg kg-1, in GM soybean values ranged from ND to 2.81 mg kg-1 and in GM cotton ranged from ND to 1.78 mg kg-1. AMPA residues levels indicated a correlation with the glyphosate residues. Glyphosate residues levels in soybean and corn grains and cotton seeds were within the Maximum Residue Limits (MRLs) established by ANVISA and Codex Alimentarius.

https://doi.org/10.1590/0103-8478cr20190244 1592 downloads

Assessment of resistance in common bean to Fusarium oxysporum f. sp. phaseoli using different inoculation and evaluation methods
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Paulino, Jean Fausto de Carvalho

; Almeida, Caléo Panhoca de

; Gonçalves, Gabriel de Moraes Cunha

; Bueno, César Júnior

; Carbonell, Sérgio Augusto Morais

; Chiorato, Alisson Fernando

; Bechimol-Reis, Luciana Lasry

Crop Breeding and Applied Biotechnology

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2020, Volume 20 N. 3 elocation e337620311

Abstract: En | Text: En | PDF: En

Abstract This study aimed to evaluate the resistance of three common bean genotypes (BRS Estilo, A211 and Mortiño) to the Fusarium oxysporum f. sp. phaseoli using different inoculation methods such as the root-dip, the colonized toothpick, and the agar-based disk-diffusion. After 35 days of inoculation, the disease severity was assessed by vascular discoloration on the stem of the plants by two methods: using a scoring scale and measuring the length of the discoloration with the aid of a millimeter ruler (cm). Results showed that the root-dip was the most effective inoculation method. As for the method of assessing the disease severity, the scoring scale was the best one, in addition to being easier for evaluating large amounts of common bean lines.

https://doi.org/10.1590/1984-70332020v20n3n45 479 downloads

Estimación del tamaño óptimo de parcela en experimentación con batata dulce
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González, Guido Gustavo Humada

; Morais, Augusto Ramalho de

; Caballero Mendoza, César Arnaldo

; Bortolini, Juliano

; Rodrigues Liska, Gilberto

Agrociencia (Uruguay)

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nov 2018, Volume 22 N. 2 Pages 7 - 12

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen: La determinación de tamaño óptimo de parcela, en cualquier cultivo, es una de las formas de aumentar la precisión experimental y maximizar las informaciones obtenidas en un experimento. Trabajos relacionados al dimensionamiento del tamaño óptimo de parcela con batata dulce datan del año 2006, entretanto, no se registran trabajos anteriores realizados con la variedad Morotí. Por tanto, con la finalidad de ofrecer y/o actualizar informaciones referentes al dimensionamiento adecuado de parcelas para el cultivo, el objetivo de este trabajo fue estimar el tamaño óptimo de parcela en experimento de campo con batata dulce(Ipomoea batatas (L.) Lam.) utilizando tres métodos, el método de máxima curvatura modificada, el modelo linear segmentado con respuesta plato y el modelo cuadrático segmentado con respuesta plato. Para ello se hizo un ensayo de uniformidad con batata dulce variedad Morotí. La unidad experimental básica fue definida en una planta (0,30 m²), posteriormente fueron simulados ocho diferentes tamaños de parcelas y se determinó el coeficiente de variación para cada uno de los tamaños simulados. Los métodos utilizados estimaron tamaños de parcela diferentes, siendo el método de máxima curvatura modificada el que estimó el menor valor relacionado al tamaño de parcela. Los métodos basados en modelos segmentados con plato estimaron tamaños adecuados de parcela. Se recomienda utilizar 13 a 16 plantas (3,90 a 4,80 m2) por parcela.

Summary: Optimum plot size determination, in any culture, is a method to increase experimental precision and maximize the information obtained in an experiment. Research of the optimal plot size for sweet potato date back to 2006, meanwhile, no previous work with the Morotí variety has been recorded. Therefore, in order to provide and/or update information regarding the adequate sizing of plots for the crop, the goal of this study was to estimate the optimum plot size in a field experiment with sweet potatoes(Ipomoea batatas (L .) Lam.). Three methods were considered: the modified maximum curvature, the segmented linear model with plateau response, and the segmented quadratic model with plateau response. For this purpose, a uniformity trial was conducted with variety Morotí of sweet potato. The basic experimental unit was defined as one plant (0,30 m²), then eight different plot sizes were simulated and the coefficient of variation for each of the simulated sizes was determined. The results showed that the three methods estimated different optimum plot sizes, the modified maximum curvature method being the one that estimated the smaller optimum plot size. The methods based on segmented models estimated the most appropriate optimum plot sizes. Methods based on dish-segmented models estimated adequate plot sizes. It is recommended to use 13 to 16 plants (3,90 to 4,80 m²) per plot.

https://doi.org/10.31285/agro.22.2.2 1195 downloads

Correlation between physical examination and three-dimensional gait analysis in the assessment of rotational abnormalities in children with cerebral palsy
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Teixeira, Fernando Borge

; Ramalho Júnior, Amancio

; Morais Filho, Mauro César de

; Speciali, Danielli Souza

; Kawamura, Catia Miyuki

; Lopes, José Augusto Fernandes

; Blumetti, Francesco Camara

einstein (São Paulo)

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2018, Volume 16 N. 1 elocation eAO4247

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Objetivo Avaliar a correlação entre dados do exame físico relativos à rotação do quadril e torção tibial com a cinemática do plano transverso em crianças com paralisia cerebral; e determinar quais pontos no tempo e eventos do ciclo de marcha apresentam maior correlação com achados do exame físico. Métodos Um total de 195 crianças com paralisia cerebral vistas em dois laboratórios de marcha, de 2008 a 2016, foi incluído neste estudo. As medidas do exame físico incluíram rotação interna do quadril, rotação externa do quadril, ponto médio da rotação do quadril e ângulo do eixo transmaleolar. Foram selecionados seis parâmetros cinemáticos para cada segmento, para avaliar a rotação do quadril e a do pé em relação à perna durante a marcha. As correlações entre exame físico e medidas cinemáticas foram analisadas por coeficientes de correlação de Spearman, e considerou-se um nível de significância de 5%. Resultados Comparando as medidas da rotação do quadril e da cinemática do quadril, encontramos correlações moderadas a fortes para todas as variáveis (p<0,001). Os coeficientes mais altos foram observados entre o ponto médio da rotação do quadril no exame físico e a rotação do quadril na cinemática (rho range: 0,48-0,61). Correlações moderadas também foram encontradas entre a medição do ângulo do eixo transmaleolar no exame físico e a rotação do pé em relação à perna na cinemática (faixa rho: 0,44-0,56; p<0,001). Conclusão Estes achados podem ter implicações clínicas na avaliação e no tratamento de desvios da marcha do plano transverso em crianças com paralisia cerebral.

Abstract Objective To evaluate the correlation between physical examination data concerning hip rotation and tibial torsion with transverse plane kinematics in children with cerebral palsy; and to determine which time points and events of the gait cycle present higher correlation with physical examination findings. Methods A total of 195 children with cerebral palsy seen at two gait laboratories from 2008 and 2016 were included in this study. Physical examination measurements included internal hip rotation, external hip rotation, mid-point hip rotation and the transmalleolar axis angle. Six kinematic parameters were selected for each segment to assess hip rotation and shank-based foot rotation. Correlations between physical examination and kinematic measures were analyzed by Spearman correlation coefficients, and a significance level of 5% was considered. Results Comparing physical examination measurements of hip rotation and hip kinematics, we found moderate to strong correlations for all variables (p<0.001). The highest coefficients were seen between the mid-point hip rotation on physical examination and hip rotation kinematics (rho range: 0.48-0.61). Moderate correlations were also found between the transmalleolar axis angle measurement on physical examination and foot rotation kinematics (rho range 0.44-0.56; p<0.001). Conclusion These findings may have clinical implications in the assessment and management of transverse plane gait deviations in children with cerebral palsy.

https://doi.org/10.1590/s1679-45082018ao4247 3005 downloads

DOES RECTUS FEMORIS TRANSFER INCREASE KNEE FLEXION DURING STANCE PHASE IN CEREBRAL PALSY?
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Morais Filho, Mauro César de

; Blumetti, Francesco Camara

; Kawamura, Cátia Miyuki

; Lopes, José Augusto Fernandes

; Neves, Daniella Lins

; Cardoso, Michelle de Oliveira

Acta Ortopédica Brasileira

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feb 2016, Volume 24 N. 1 Pages 27 - 31

Abstract: En | Text: En | PDF: En

ABSTRACT Objective: To evaluate whether distal rectus femoris transfer (DRFT) is related to postoperative increase of knee flexion during the stance phase in cerebral palsy (CP). Methods: The inclusion criteria were Gross Motor Function Classification System (GMFCS) levels I-III, kinematic criteria for stiff-knee gait at baseline, and individuals who underwent orthopaedic surgery and had gait analyses performed before and after intervention. The patients included were divided into the following two groups: NO-DRFT (133 patients), which included patients who underwent orthopaedic surgery without DRFT, and DRFT (83 patients), which included patients who underwent orthopaedic surgery that included DRFT. The primary outcome was to evaluate in each group if minimum knee flexion in stance phase (FMJFA) changed after treatment. Results: The mean FMJFA increased from 13.19° to 16.74° (p=0.003) and from 10.60° to 14.80° (p=0.001) in Groups NO-DRFT and DRFT, respectively. The post-operative FMJFA was similar between groups NO-DRFT and DRFT (p=0.534). The increase of FMJFA during the second exam (from 13.01° to 22.51°) was higher among the GMFCS III patients in the DRFT group (p<0.001). Conclusion: In this study, DRFT did not generate additional increase of knee flexion during stance phase when compared to the control group. Level of Evidence III, Retrospective Comparative Study.

https://doi.org/10.1590/1413-785220162401145765 1238 downloads

II Diretriz de Ressonância Magnética e Tomografia Computadorizada Cardiovascular da Sociedade Brasileira de Cardiologia e do Colégio Brasileiro de Radiologia
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Sara, Leonardo

; Szarf, Gilberto

; Tachibana, Adriano

; Shiozaki, Afonso Akio

; Villa, Alexandre Volney

; Oliveira, Amarino Carvalho de

; Albuquerque, Andrei Skromov de

; Rochitte, Carlos Eduardo

; Nomura, César Higa

; Azevedo, Clerio Francisco

; Jasinowodolinski, Dany

; Tassi, Eduardo Marinho

; Medeiros, Fabio de Morais

; Kay, Fernando Uliana

; Junqueira, Flávia Pegado

; Azevedo, Guilherme S. A.

; Monte, Guilherme Urpia

; Pinto, Ibraim Masciarelli Francisco

; Gottlieb, Ilan

; Andrade, Joalbo

; Lima, João A. C.

; Parga Filho, José Rodrigues

; Kelendjian, Juliana

; Fernandes, Juliano Lara

; Iquizli, Leonardo

; Correia, Luis C. L.

; Quaglia, Luiz Augusto

; Gonçalves, Luiz Flavio Galvão

; Ávila, Luiz Francisco

; Zapparoli, Marcello

; Hadlich, Marcelo

; Nacif, Marcelo Souto

; Barbosa, Márcia de Melo

; Minami, Márcio Hiroshi

; Bittencourt, Marcio Sommer

; Siqueira, Maria Helena Albernaz

; Silva, Marly Conceição

; Lopes, Marly Maria Uellendahl

; Marques, Mateus Diniz

; Vieira, Mônica La Rocca

; Coellho Filho, Otávio Rizzi

; Schvartzman, Paulo R.

; Santos, Raul D.

; Cury, Ricardo C.

; Loureiro, Ricardo

; Cury, Roberto Caldeira

; Sasdelli Neto, Roberto

; Macedo, Robson

; Cerci, Rodrigo Julio

; Faria Filho, Rui Alberto de

; Cardoso, Sávio

; Naves, Thiago

; Magalhães, Tiago Augusto

; Senra, Tiago

; Burgos, Ursula Maria Moreira Costa

; Moreira, Valéria de Melo

; Ishikawa, Walther Yoshiharu

Arquivos Brasileiros de Cardiologia

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dec 2014, Volume 103 N. 6 Pages 1 - 86

Abstract: Pt | Text: Pt | PDF: Pt

https://doi.org/10.5935/abc.2014S006 67586 downloads

10.

Most frequent gait patterns in diplegic spastic cerebral palsy
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Morais Filho, Mauro César de

; Kawamura, Cátia Miyuki

; Lopes, José Augusto Fernandes

; Neves, Daniella Lins

; Cardoso, Michelle de Oliveira

; Caiafa, Jordana Brandão

Acta Ortopédica Brasileira

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aug 2014, Volume 22 N. 4 Pages 197 - 201

Abstract: En | Text: En | PDF: En

OBJECTIVE: To identify gait patterns in a large group of children with diplegic cerebral palsy and to characterize each group according to age, Gross Motor Function Classification System (GMFCS) level, Gait Deviation Index (GDI) and previous surgical procedures. METHODS: One thousand eight hundred and five patients were divided in seven groups regarding observed gait patterns: jump knee, crouch knee, recurvatum knee, stiff knee, asymmetric, mixed and non-classified. RESULTS: The asymmetric group was the most prevalent (48.8%). The jump knee (9.6 years old) and recurvatum (9.4 years old) groups had mean age lower than the other groups. The lowest GDI (43.58) was found in the crouch group. There were more children classified within GMFCS level III in the crouch and mixed groups. Previous surgical procedures on the triceps surae were more frequent in stiff knee and mixed groups. The jump knee group received less and the stiff-knee group more surgical procedures at hamstrings than others. CONCLUSIONS: The asymmetrical cases were the most frequent within a group of diplegic patients. Individuals with crouch gait pattern were characterized by the lowest GDI and the highest prevalence of GMFCS III, while patients with stiff knee exhibited a higher percentage of previous hamstring lengthening in comparison to the other groups. Level of Evidence III, Retrospective Comparative Study.

https://doi.org/10.1590/1413-78522014220400942 46405 downloads

11.

Localization of metastasis within the sentinel lymph node biopsies: a predictor of additional axillary spread of breast cancer?
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Alvarenga, César Augusto

; Santos, César Cabello dos

; Alvarenga, Marcelo

; Paravidino, Paula Itagyba

; Morais, Sirlei Siani

; Brenelli, Henrique Benedito

; Carvalho, Filomena Marino de

Revista Brasileira de Ginecologia e Obstetrícia

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nov 2013, Volume 35 N. 11 Pages 483 - 499

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO:Explorar a relação entre características morfológicas e localização histológica da metástase dentro dos linfonodos sentinelas (LS) e disseminação axilar em mulheres com câncer de mama. MÉTODOS: Foram selecionados 119 pacientes com LS positivo, submetidas à dissecação completa dos linfonodos axilares entre Julho de 2002 a Março de 2007. Foram recuperados a idade das pacientes e o tamanho do tumor primário. No tumor primário, avaliamos os graus histológico e nuclear e a invasão vascular peritumoral (IVP). Nos LS, avaliamos o tamanho da metástase, sua localização no linfonodo, o número de focos metastáticos, número de linfonodos envolvidos e a extensão extranodal. RESULTADOS: Cinquenta e um (42,8%) pacientes tiveram metástases adicionais confirmadas nos linfonodos não sentinelas (LNS). Alto grau histológico, IVP, metástase intraparenquimatosa, extensão extranodal e tamanho da metástase foram associados com LNS positivos. Metástase afetando a cápsula do LS foi associada com baixo risco de incidência de metástase adicional. Após análise multivariada, IVP e tamanho da metástase no LS foram os fatores de risco mais importantes para metástases adicionais nos LNS. CONCLUSÕES:O risco de envolvimento adicional dos LNS é maior em pacientes com IVP e tal risco aumenta progressivamente de acordo com a localização histológica da metástase no LS, que inicia na cápsula, onde aporta o linfático aferente, e termina no lado oposto, promovendo a extensão extranodal. Tamanho de metástase maior ou igual a 6,0 mm revela maior risco de metástase nos LNS.

PURPOSE: To explore the relationship between morphological characteristics and histologic localization of metastasis within sentinel lymph nodes (SLN) and axillary spread in women with breast cancer. METHODS: We selected 119 patients with positive SLN submitted to complete axillary lymph node dissection from July 2002 to March 2007. We retrieved the age of patients and the primary tumor size. In the primary tumor, we evaluated histologic and nuclear grade, and peritumoral vascular invasion (PVI). In SLNs we evaluated the size of metastasis, their localization in the lymph node, number of foci, number of involved lymph nodes, and extranodal extension. RESULTS: Fifty-one (42.8%) patients had confirmed additional metastasis in non-sentinel lymph nodes (NLSN). High histologic grade, PVI, intraparenchymatous metastasis, extranodal neoplastic extension and size of metastasis were associated with positive NLSN. SLN metastasis affecting the capsule were associated to low risk incidence of additional metastasis. After multivariate analysis, PVI and metastasis size in the SLN remained as the most important risk factors for additional metastasis. CONCLUSIONS:The risk of additional involvement of NSLN is higher in patients with PVI and it increases progressively according the histologic localization in the lymph node, from capsule, where the afferent lymphatic channel arrives, to the opposite side of capsule promoting the extranodal extension. Size of metastasis greater than 6.0 mm presents higher risk of additional lymph node metastasis.

22098 downloads

12.

Effect of pre-heated dual-cured resin cements on the bond strength of indirect restorations to dentin
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Morais, Alexandre

; Santos, Alline Rachid Abreu dos

; Giannini, Marcelo

; Reis, Andre Figueiredo

; Rodrigues, José Augusto

; Arrais, César Augusto Galvão

Brazilian Oral Research

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apr 2012, Volume 26 N. 2 Pages 170 - 176

Abstract: En | Text: En | PDF: En

This study evaluated the effects of resin luting agents (LA) polymerized using increased temperature on the in vitro microtensile bond strength (mTBS) of indirect restorations to dentin. The occlusal dentin surfaces of 40 human third molars were exposed and flattened. The teeth were assigned to 8 groups (n = 5) according to the LA temperature (25°C o r 50°C), curing mode (dual- or self-curing mode), and product (Excite DSC/Variolink II [VII] and XP Bond/Calibra [Cal]). The bonding agents were applied to the dentin surfaces according to manufacturers' instructions. For preheated groups, the LAs were heated to 50°C, subsequently mixed on a heated stirrer surface, and applied to the previously heated pre-polymerized resin discs (2 mm thickness, TPH-Spectrum). The discs were bonded to the dentin surfaces, and the LAs were either exposed to a curing light according to manufacturers' instructions or allowed to self-cure. Specimens were stored in relative humidity at 37°C for 7 days. Specimens were mesio-distally and bucco-lingually sectioned to obtain multiple bonded beams with a 1-mm² cross-sectional area for mTBS testing. Data (MPa) were analyzed by 2-way ANOVA and Tukey's post hoc test (a = 5%) for each product. Specimen failure patterns were analyzed using a scanning electron microscope. VII groups showed higher mTBS at 50°C than at 25°C regardless of curing mode (p = 0.05). Cal groups showed similar mTBS at 25°C and 50°C in all activation modes. The use of some dual-polymerizing LAs at 50°C may improve the mTBS of indirect restorations to dentin.

4505 downloads

13.

Prevenção de isquemia hepática normotérmica durante perfusão hepática in situ com três diferentes soluções de preservação: análise experimental através de termografia por radiação infravermelha em tempo real
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Matias, Jorge Eduardo Fouto

; Morais, Felipe Augusto

; Kato, Daniel Massamatsu Pianovski

; Koziak, Vanessa

; Brioschi, Marcos Leal

; Tambara, Elizabeth Milla

; Agulham, Miguel Ângelo

; Coelho, Júlio César Uili

Revista do Colégio Brasileiro de Cirurgiões

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jun 2010, Volume 37 N. 3 Pages 211 - 217

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: Determinar a aplicabilidade da termografia por radiação infravermelha no monitoramento da perfusão hepática in situ com diferentes soluções de preservação. MÉTODOS: 24 ratos Wistar machos adultos, foram distribuidos aleatoriamente em quatro grupos de seis animais: grupo EC; ®grupo CUST; ®grupo CEL e grupo RL. Todas as soluções estavam resfriadas à exatamente 4ºC e posicionadas 20 cm acima do nível do fígado. As imagens infravermelhas, com as respectivas avaliações de temperatura da superfície hepática, foram captadas em tempo real : imediatamente após a laparotomia; após a canulação dos vasos e imediatamente antes da infusão; a cada minuto cronometrado após início da infusão até o quinto minuto de infusão. As médias de temperaturas de cada momento foram comparadas intra e intergrupos através do teste da Diferença entre médias de distribuição normal, com nível de significância p 0,05. RESULTADOS: Houve diferença entre as temperaturas do momento da laparotomia e imediatamente após a canulação; entre este último e após o primeiro minuto de perfusão; e entre as medidas ao final do primeiro e do quinto minutos de perfusão em todos os grupos. O grupo CEL mostrou diferença significativa adicional entre as temperaturas medidas ao final do primeiro e do segundo minutos. CONCLUSÃO: Foi possível avaliar o resfriamento hepático durante a perfusão das soluções de preservação utilizando-se a radiação infravermelha. As soluções tiveram comportamento semelhante entre si, com a solução Celsior® mostrando potencial adicional de resfriamento significativo até ao final do segundo minuto.

OBJECTIVE: To establish the usefulness of infrared radiation thermography on monitoring in situ liver perfusion with different preservation solutions during liver harvesting. METHODS: Twenty-four adult male Wistar rats, weighing 385.31g were randomly divided into four groups of six animals each according to the solution used to perfuse the liver (Euro-Collins® solution - EC group; Custodiol® solution - CUST group; Celsior® solution - CEL group and Ringer-Lactate solution - RL group). Under inhalatory ether anesthesia, animals were submitted to upper transversal laparotomy, exposure of median and left-lateral hepatic lobes, heparin injection (500 UI/Kg) through infrahepatic vena cava, portal vein infusion through 18G catheter of cold (4°C) solution according to the group of study. Infrared images, with respective temperature avaliations from hepatic surface, were picked up in real time by Therma CAM SC500® infrared camera positioned at constant distance from three fixed points of the diaphragmatic surface of median and left lateral lobes at the following moments regarding liver perfusion: immediately after laparotomy; after portal vein cannulation and immediately before solution infusion; at each minute from the beginning of liver perfusion during five minutes. Mean temperatures of each moment were compared intra and intergroups with the difference between means test with normal distribution, with significance level of 5% (p=0.05). RESULTS: There was statistically significant difference of means temperatures between the moment of laparotomy and immediately after cannulation; between this later and after the first minute of perfusion; and between the first and fifth minutes of infusion in all groups of study in a similar way. CEL group showed additional difference between the first and second minutes means temperatures. Intergroup comparison showed Euro-Collins solution with significant less cooling power when compared to all others solutions. CONCLUSION: It was possible to follow the liver cooling process during preservation solutions perfusion using infrared radiation images. Preservation solutions had similar behaviors, with Celsior® solution showing additional cooling power until the second minute of perfusion. Euro-Collins solution had less cooling power than other solutions studied.

5456 downloads

14.

Estabilidade aeróbia de silagens de capim-mombaça tratadas com Lactobacillus buchneri
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Ávila, Carla Luiza da Silva

; Pinto, José Cardoso

; Figueiredo, Henrique César Pereira

; Morais, Augusto Ramalho de

; Pereira, Odilon Gomes

; Schwan, Rosane Freitas

Revista Brasileira de Zootecnia

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may 2009, Volume 38 N. 5 Pages 779 - 787

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Objetivou-se avaliar o efeito da adição de duas cepas de Lactobacillus buchneri sobre a estabilidade aeróbia de silagens de capim-mombaça. O experimento foi conduzido em delineamento inteiramente casualizado com três repetições, em esquema de parcelas subdivididas no tempo, de modo que os tratamentos (silagens sem inoculantes e com inoculante experimental e comercial) foram aleatorizados nas parcelas e os tempos de avaliação aeróbia das silagens (0, 2, 8 ou 10 dias após a abertura dos silos) nas subparcelas. Após 90 dias de armazenamento, os silos foram abertos e amostras de aproximadamente 2,5 kg foram retiradas para avaliação da estabilidade aeróbia com base na temperatura de cada amostra. As mudanças químicas e microbiológicas foram avaliadas. Após abertura dos silos, verificou-se aumento nas populações de bactérias do ácido lático, leveduras e fungos filamentosos. Houve aumento nos valores de pH resultante da redução da concentração dos ácidos orgnicos e de aumentos dos teores de matéria seca e proteína bruta. A adição de L. buchneri às silagens melhorou sua estabilidade, enquanto a silagem sem inoculante apresentou estabilidade de 55 horas. As silagens inoculadas não ultrapassaram essa temperatura limite em um período de 10 dias. A melhoria da estabilidade foi ocasionada pela associação de altas concentrações de cido acético ao ácido propiônico e pela menor concentração de ácido lático no momento de abertura, o que inibiu a proliferação de fungos filamentosos e leveduras após abertura dos silos.

This study aimed to evaluate the effect of the addition of two L. buchneri strains on the aerobic stability of the Mombaça grass silages. The experiment was carried out in a completely randomized design with three replicates in split-plot in time scheme, so that the treatments (silages without inoculant and with experimental or commercial inoculants) were randomized on plot and in times of aerobic evaluation of the silages (0, 2, 8, and 10 days after the opening of the silos) as subplots. After an storage period of 90 days, the silos were opened and samples of about 2,5 kg were collected for the evaluation of the aerobic stability based on the temperature for each sample. The chemical and microbiological changes in the silages were evaluated. As soon as the silos were opened, changes in the silages were observed, with increases in the lactic acid bacteria, yeast and filamentous fungus populations. Also, the pH values increased due to the reduction in the organic acids concentration and to the increase in the DM and CP contents. The addition of L.buchneri to the silages increased their stability. The silages without inoculant was stable for 55 hours. The inoculated silages remained stable for 10 days. The improved stability was a consequence of an association of high acetic acid concentration with propionic acid and lower lactic acid concentration at the moment of silo opening, which resulted in inhibition of yeasts and molds.

5409 downloads

15.

Efeito da adubação nitrogenada e irrigação sobre a composição químico-bromatológica das lâminas foliares e da planta inteira de capim-elefante sob pastejo
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Mistura, Claudio

; Fonseca, Dilermando Miranda da

; Moreira, Luciano de Melo

; Fagundes, Jailson Lara

; Morais, Rodrigo Vieira

; Queiroz, Augusto César de

; Ribeiro Júnior, José Ivo

Revista Brasileira de Zootecnia

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dec 2007, Volume 36 N. 6 Pages 1707 - 1714

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Esta pesquisa foi desenvolvida com o objetivo de avaliar o efeito da adubação nitrogenada (100, 200, 300, 400 kg/ha/ano de N) e da irrigação (área irrigada - AI e não-irrigada - ANI) sobre a composição quimico-bromatológica da planta inteira (PI) (colmo+folha) e das lâminas foliares dos perfilhos basais não-decapitados (LF-PBNd) e decapitados (LF-PBd) do capim-elefante cv. Napier. Adotou-se delineamento experimental em blocos casualizados com três repetições, em que a unidade experimental consistiu de parcelas (piquetes de 300 m²), nas quais foram aplicados os tratamentos. Os teores de PB apresentaram valores proporcionais às doses de nitrogênio, observando-se os maiores valores, no período das águas, nas LF-PBNd e LF-PBd. Os resíduos das doses N aplicados no período chuvoso tanto na AI como ANI elevaram os teores de PB nas lâminas foliares das duas categorias de perfilhos, mas não sobre os teores de FDN e FDA. A irrigação proporcionou teores de FDN e FDA mais elevados nas LF-PBNd e LF-PBd e menores na planta inteira, porém, não se observou efeito sobre os teores de PB.

This research was carried out to evaluate the chemical composition of elephantgrass cv. Napier in the whole plant (WP) (stem+leaf) and in the leaf blade of non-decapitated basal tillers (SF-NDBT) and leaf blade of decapitated basal tillers (SF-DBT), fertilized with different N doses (100, 200, 300, and 400 kg/ha/year of N) in an irrigated area (IA) and in a non-irrigated area (NIA). Treatments were analyzed in a randomized block design with three replicates. The CP concentrations were proportional to the N doses with greater CP concentration in the rainy season in fractions SF-NDBT and SF-DBT. Fertilizer residues of N doses applied in the rainy season, both in IA and NIA, increased CP concentration in the leaf blade of the two tiller categories, but did not affect NDF and ADF concentration. Irrigation increased concentration of NDF and ADF in SF-NDBT and SF-DBT and reduced it in the WP, with no effect on CP concentration.

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