In this work, 25,806 potentially amplifiable microsatellite loci (PAL) were identified in pejerrey, (Odontesthes humensis), with 21% of dinucleotide, 22% trinucleotide, 37% tetranucleotide, 13% pentanucleotide and 7% hexanucleotide. Of the total loci, 167 were classified as "Best PAL", more likely to be variables in populations. The results show that with a small coverage of the genome it was possible to identify a large number of microsatellite loci.

The aim of this study was to determine the correlation between total nitrite/nitrate concentrations (NOx) and the kinetic parameters of monoamine oxidase enzymes (MAO-A and MAO-B) and semicarbazide-sensitive amine oxidase (SSAO) in human mesenteric arteries. Arteries were from non-diabetic and type 2 diabetic patients with sigmoid or rectum carcinoma for whom surgery was the first option and who were not exposed to neo-adjuvant therapy. Segments of human inferior mesenteric arteries from non-diabetic (61.1 ± 8.9 years old, 7 males and 5 females, N = 12) and type 2 diabetic patients (65.8 ± 6.2 years old, 8 males and 4 females, N = 12) were used to determine NOx concentrations and the kinetic parameters of MAO-A, MAO-B and SSAO by the Griess reaction and by radiochemical assay, respectively. The NOx concentrations in arteries from diabetic patients did not differ significantly from those of the non-diabetic group (10.28 ± 4.61 vs 10.71 ± 4.32 nmol/mg protein, respectively). In the non-diabetic group, there was a positive correlation between NOx concentrations and MAO-B parameters: Km (r = 0.612, P = 0.034) and Vmax (r = 0.593, P = 0.042), and a negative correlation with the SSAO parameters: Km (r = -0.625, P = 0.029) and Vmax (r = -0.754, P = 0.005). However, in the diabetic group no correlation was found between NOx concentrations and the three kinetic parameters of the enzymes. These results suggest an important function of sympathetic nerves and vascular NOx concentrations in arteries of non-diabetic patients. Thus, these results confirm the importance of a balance between oxidants and antioxidants in the maintenance of vascular homeostasis to prevent oxidative stress.

O presente estudo investiga as ideias de corpo humano que são transmitidas e percebidas por professores de Educação Física (EF), em aulas do Ensino Fundamental, em escolas da região sul da cidade de São Paulo. Para isso, 15 professores responderam, por escrito, às seguintes questões: Que ideias sobre o corpo humano transmite nas suas aulas de EF? Você percebe reações de seus alunos a essas ideias? Os resultados obtidos por meio da análise de conteúdo das respostas revelaram que os professores, quando transmitem ideias de corpo restritas à dimensão física, percebem que as reações de seus alunos se direcionam para aspetos concretos do corpo e para aspetos relacionados ao comportamento dos organismos; quando transmitem ideias de corpo num enfoque na sua dimensão social, percebem que seus alunos demonstram autonomia em termos de aspetos socioculturais e, finalmente, quando transmitem ideias de corpo com um foco psicológico, percebem que seus alunos relacionam as atividades físicas aos aspetos cognitivos. De entre os professores pesquisados, apenas um transmite aos seus alunos ideias de corpo humano por meio dos saberes físico, social e psíquico inter-relacionados.

The present study investigates the ideas of the human body transmitted and grasped by the teachers of Physical Education in Basic Education classes. It was carried a research with Physical Education teachers using the following questions: What ideas about the human body are you transmitting in your Physical Education classes? Do you perceive the reactions of your students to these ideas? The analyses of the answers were methodologically followed in these moments: a) reading of the descriptions, b) elaboration of the units of meaning; c) ideographic analysis; d) nomothetic analysis. Results showed that teachers of Physical Education when transmitting ideas of restricted body in the physical dimension, they realized that the reactions of their students were directed to the concrete aspects of the body and to the aspects related to the behavior of the organisms; when they transmitted ideas of body focusing in the social dimension, they realized that their students demonstrated autonomy in social and cultural aspects. Finally, when they transmitted ideas of body in more including ways, they realized that their students related the physical activities to cognitive aspects. The results also showed that among the researched teachers, only one transmitted to the students the idea of the human body through inter-related physical, social and psychological knowledge.