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au:OLIVEIRA, ADRIANO S.

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Antimicrobial effect of phenolic-rich jaboticaba peel aqueous extract on Staphylococcus aureus and Escherichia coli
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Fleck, Nataís

; Oliveira, Wemerson Castro de

; Padilha, Rosiele Lappe

; Brandelli, Adriano

; Sant’Anna, Voltaire

Brazilian Journal of Food Technology

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2023, Volume 26 elocation e2022087

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract Jaboticaba peels are an important source of health-benefit and antimicrobial compounds. The present work aims to evaluate concentration of polyphenolics and the mode of action of aqueous extract from jaboticaba peels against Staphylococcus aureus and Escherichia coli. Jaboticaba peel extract showed minimum inhibitory concentration and minimum bactericidal concentration against S. aureus of 5.1 g L-1 and 10.1 g L-1, respectively; meanwhile, against E. coli the parameters were 2.0 g L-1 and 3.4 g L-1. Kinetics of viable cell counts indicated a bacteriolytic action against both bacteria and Scanning Electron Microscopy (SEM) showed that jaboticaba peel extract causes subtle morphological changes in bacterial cells. Concentration of total polyphenols in the extract was 1535.04±36.05 mg of gallic acid equivalent (GAE) mL-1, monomeric anthocyanins was 14.52 ± 0.98 mg of cyanidin 3-glucoside mL-1, condensed tannins was 0.49 ± 0.05 mg of epicatechin equivalent mL-1 and phenolic acids was 80.04 ± 4.11 mg of caffeic acid equivalent (CAE) mL-1, which have demonstrated well-documented antibacterial activity. In conclusion, jaboticaba peel aqueous extract may be an interesting natural preservative to control Gram-positive and Gram-negative bacteria growth when interacting with the bacteria cell wall.

Resumo As cascas de jabuticaba são uma importante fonte de benefícios à saúde e compostos com atividade antimicrobiana. O presente trabalho tem como objetivo avaliar a concentração de polifenóis e o modo de ação do extrato aquoso da casca da jabuticaba contra Staphylococcus aureus e Escherichia coli. O extrato da casca da jabuticaba apresentou concentração inibitória mínima e concentração bactericida mínima contra S. aureus de 5,1 g L-1 e 10,1 g L-1, respectivamente; já contra E. coli, esses parâmetros foram 2,0 g L-1 e 3,4 g L-1. A cinética da contagem de células viáveis indica uma ação bacteriolítica contra ambas as bactérias e a microscopia eletrônica de varredura mostrou que o extrato da casca da jabuticaba causa sutis alterações morfológicas nas células bacterianas. A concentração de polifenóis totais no extrato foi 1.535,04±36,05 mg ácido gálico equivalente mL-1; antocianinas monoméricas, 14,52±0,98 mg cianidina 3-glicosídeo mL-1; taninos condensados, 0,49 ± 0,05 mg equivalente epicatequina mL-1, e ácidos fenólicos, 80,04±4,11 mg ácido cafeico equivalente mL-1, compostos que já demonstraram atividade antibacteriana bem documentada. Em conclusão, o extrato aquoso da casca de jabuticaba mostra-se um bioconservante interessante para o controle de bactérias Gram-positivas e Gram-negativas, e interage com a parede celular bacteriana.

https://doi.org/10.1590/1981-6723.08722

Milonine attenuates the lipopolysaccharide-induced acute lung injury in mice by modulating the Akt/NF-κB signaling pathways
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BERNARDO, LARISSA R.

; FERREIRA, LAÉRCIA KARLA D.P.

; FERREIRA, LARISSA A.M.P.

; VIEIRA, COSMO ISAÍAS D.

; OLIVEIRA, JOÃO BATISTA DE

; LIMA, LOUISE M. DE

; ALVES, ADRIANO FRANCISCO

; ARAÚJO, RUBENS S.

; MAIA, MAYARA S.

; SCOTTI, MARCUS T.

; BARBOSA FILHO, JOSÉ MARIA

; PIUVEZAM, MARCIA REGINA

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Suppl. 4 elocation e20211327

Abstract: En | Text: En | PDF: En

Abstract Acute lung injury is an inflammation that triggers acute respiratory distress syndrome with perialveolar neutrophil infiltration, alveolar-capillary barrier damage, and lung edema. Activation of the toll-like receptor 4 complex (TLR4/MD2) and its downstream signaling pathways are responsible for the cytokine storm and cause alveolar damage. Due to the complexity of this pulmonary inflammation, a defined pharmacotherapy has not been established. Thus, this study evaluated the anti-inflammatory potential of milonine, an alkaloid of Cissampelos sympodialis Eichl, in an experimental model of lung inflammation. BALB/c mice were lipopolysaccharide-challenged and treated with milonine at 2.0 mg/kg. Twenty-four hours later, the bronchoalveolar fluid, peripheral blood, and lungs were collected for cellular and molecular analysis. The milonine treatment decreased the cell migration (mainly neutrophils) to the alveoli, the pulmonary edema, and the cytokine levels (IL-1β, IL-6, TNF-α). The systemic IL-6 level was also reduced. The milonine docking analysis demonstrated hydrophobic interaction at TLR4/MD2 groove with Ile124 and Phe126 amino acids. Indeed, the alkaloid downregulated the kinase-Akt and NF-κB through TLR4/MD2. Therefore, milonine is an effective inflammatory modulator being a potential molecule for the treatment of lung inflammation.

https://doi.org/10.1590/0001-3765202220211327

Effect of combining Zingiber officinale and Juglans regia extracts on Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis: antibiofilm action and low toxicity
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SILVA, LEONARDO A. DA

; RAMOS, LUCAS P.

; SILVA, TATIANE A.

; LAPENA, SIMONE A.B. DE

; SANTOS, CARLOS EDUARDO R.

; HASNA, AMJAD A.

; BRESSANE, ADRIANO

; OLIVEIRA, LUCIANE D. DE

Anais da Academia Brasileira de Ciências

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2022, Volume 94 N. 4 elocation e20201133

Abstract: En | Text: En | PDF: En

Abstract Objective was evaluated the therapeutic effect of Juglans regia (J) and Zingiber officinale (Z) extracts, alone or associated (Z75% + J25%, Z50% + J50% and Z25% + J75%) applied on planktonic cultures and biofilms of Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus, as well as analyzing the cytotoxic effects of plant extracts on mouse macrophages (Raw 264-7). Broth microdilution assay was performed (M7-A6 - CLSI). Anti-biofilm activities and cytotoxicity on Raw 264-7 were studied using MTT assay and scanning electron microscopy. ANOVA with post-hoc Tukey HSD applied for parametric data and Kruskal-Wallis with Conover-Iman test, for non-parametric (p<0.05). On P. acnes biofilm, Z50% + J50% reduced 46.9% in 5 min and Z25% + J75% reduced 74.1% in 24hs. On S. aureus, Z75% + J25% reduced 23.1% in 5 min Z25% +J75% reduced 79.4% in 24hs. On S. epidermidis, Z75% + J25% reduced 74.6% in 5 min and 82.05% in 24 h. The treatments on macrophages for 24 h promoted a maximum reduction by 14,5% for groups of extracts associations. On multispecies biofilm, Z75%+J25% reduced 84.3% in 24 h. In conclusion association of glycolic extracts provided therapeutic effect, demonstrated antimicrobial activity and low cytotoxicity.

https://doi.org/10.1590/0001-3765202220201133

COVID-19: The question of genetic diversity and therapeutic intervention approaches
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Figueiredo, David Livingstone Alves

; Ximenez, João Paulo Bianchi

; Seiva, Fábio Rodrigues Ferreira

; Panis, Carolina

; Bezerra, Rafael dos Santos

; Ferrasa, Adriano

; Cecchini, Alessandra Lourenço

; Medeiros, Alexandra Ivo de

; Almeida, Ana Marisa Fusco

; Ramão, Anelisa

; Boldt, Angelica Beate Winter

; Moya, Carla Fredrichsen

; Chin, Chung Man

; Paula, Daniel de

; Rech, Daniel

; Gradia, Daniela Fiori

; Malheiros, Danielle

; Venturini, Danielle

; Tavares, Eliandro Reis

; Carraro, Emerson

; Ribeiro, Enilze Maria de Souza Fonseca

; Pereira, Evani Marques

; Tuon, Felipe Francisco

; Follador, Franciele Aní Caovilla

; Fernandes, Glaura Scantamburlo Alves

; Volpato, Hélito

; Cólus, Ilce Mara de Syllos

; Oliveira, Jaqueline Carvalho de

; Rodrigues, Jean Henrique da Silva

; Santos, Jean Leandro dos

; Visentainer, Jeane Eliete Laguila

; Brandi, Juliana Cristina

; Serpeloni, Juliana Mara

; Bonini, Juliana Sartori

; Oliveira, Karen Brajão de

; Fiorentin, Karine

; Lucio, Léia Carolina

; Faccin-Galhardi, Ligia Carla

; Ferreto, Lirane Elize Defante

; Lioni, Lucy Megumi Yamauchi

; Consolaro, Marcia Edilaine Lopes

; Vicari, Marcelo Ricardo

; Arbex, Marcos Abdo

; Pileggi, Marcos

; Watanabe, Maria Angelica Ehara

; Costa, Maria Antônia Ramos

; Giannini, Maria José S. Mendes

; Amarante, Marla Karine

; Khalil, Najeh Maissar

; Lima Neto, Quirino Alves de

; Herai, Roberto H.

; Guembarovski, Roberta Losi

; Shinsato, Rogério N.

; Mainardes, Rubiana Mara

; Giuliatti, Silvana

; Yamada-Ogatta, Sueli Fumie

; Gerber, Viviane Knuppel de Quadros

; Pavanelli, Wander Rogério

; Silva, Weber Claudio da

; Petzl-Erler, Maria Luiza

; Valente, Valeria

; Soares, Christiane Pienna

; Cavalli, Luciane Regina

; Silva Jr, Wilson Araujo

Genetics and Molecular Biology

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2021, Volume 44 N. 1 elocation e20200452

Abstract: En | Text: En | PDF: En

Abstract Coronavirus disease 2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome Coronavirus type 2 (SARS-CoV-2), is the largest pandemic in modern history with very high infection rates and considerable mortality. The disease, which emerged in China’s Wuhan province, had its first reported case on December 29, 2019, and spread rapidly worldwide. On March 11, 2020, the World Health Organization (WHO) declared the COVID-19 outbreak a pandemic and global health emergency. Since the outbreak, efforts to develop COVID-19 vaccines, engineer new drugs, and evaluate existing ones for drug repurposing have been intensively undertaken to find ways to control this pandemic. COVID-19 therapeutic strategies aim to impair molecular pathways involved in the virus entrance and replication or interfere in the patients’ overreaction and immunopathology. Moreover, nanotechnology could be an approach to boost the activity of new drugs. Several COVID-19 vaccine candidates have received emergency-use or full authorization in one or more countries, and others are being developed and tested. This review assesses the different strategies currently proposed to control COVID-19 and the issues or limitations imposed on some approaches by the human and viral genetic variability.

https://doi.org/10.1590/1678-4685-gmb-2020-0452

CONSTITUENTS FROM ROOTS OF Maytenus distichophylla, ANTIMICROBIAL ACTIVITY AND TOXICITY FOR CELLS AND Caenorhabditis elegans
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Morales, Shirley A. T.

; Aguilar, Mariana G. de

; Pereira, Rafael C. G.

; Duarte, Lucienir P.

; Sousa, Grasiely F.

; Oliveira, Djalma M. de

; Evangelista, Fernanda C. G.

; Sabino, Adriano P.

; Viana, Roberta O.

; Alves, Viviane S.

; Vieira-Filho, Sidney A.

Química Nova

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sep 2020, Volume 43 N. 8 Pages 1066 - 1073

Abstract: En | Text: En | PDF: En

Maytenus distichophylla is a medicinal species used in Northeast of Brazil. The hexane (HE), chloroform (CE), ethyl acetate (EAE) and methanol (ME) extracts and compounds from its roots were evaluated for their protective activity against Staphylococcus aureus and Candida albicans. The cytotoxicity for chronic myeloid leukemia (K562), acute monocytic leukemia (THP-1), and peripheral blood mononuclear cells of normal individuals (PBMC) cells was established by MTT method using etoposide as standard. The in vivo toxicity of samples was determined using Caenorhabditis elegans model. From HE and CE were isolated: friedelan-3-one (1), b-sitosterol (2), 3-oxo-olean-9(11),12-diene (3), a mixture of pristimerin (4) and 11a-hydroxylup-20(29)-en-3-one (5), 30-hydroxylup-20(29)-en-3-one (6), friedelane-3,7-dione (7), tingenone (8) and triacylglycerol (9). The structures of 1-9 were confirmed by spectral data. All samples reduced the viability of S. aureus and present no effect against C. albicans. The HE, CE, mixture of 4/5 and 8 reduced 75% of S. aureus viability. Cytotoxic effect for K562 and THP-1 cells was caused by compounds 1, 2 and 8. All samples displayed selectivity for leukemic cells and low toxicity to PBMC cells, suggesting their potential as anticancer agents. Extracts and compounds were non-toxic to L1 larvae of C. elegans. However, most of them reduced significantly young adult worm’s survival, being considered as potential nematicides.

https://doi.org/10.21577/0100-4042.20170591 344 downloads

COVID-19 e Eventos Coronários Agudos – Danos Colaterais. Um Relato de Caso
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Ritt, Luiz Eduardo Fonteles

; Viana, Mateus S.

; Feitosa, Gustavo Freitas

; Oliveira, Adriano Martins de

; Souza, Fabio Solano

; Darzé, Eduardo Sahade

Arquivos Brasileiros de Cardiologia

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jun 2020, Volume 114 N. 6 Pages 1072 - 1075

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.36660/abc.20200329 1944 downloads

Design, Synthesis and Antitubercular Activity of Novel Isoniazid-Cyclic-Amine-Azachalcones Hybrids
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Oliveira, Jefferson R. S.

; Shiguemoto, Cristiane Y. K.

; Neves, Amarith R. das

; Moreira, Flora M. F.

; Gomes, Giovana B.

; Perdomo, Renata T.

; Barbosa, Sandro L.

; Guerrero Jr., Palimécio G.

; Croda, Júlio

; Baroni, Adriano C. M.

Journal of the Brazilian Chemical Society

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jun 2020, Volume 31 N. 6 Pages 1284 - 1295

Abstract: En | Text: En | PDF: En

In this work, it is described the design of twenty-four heterocyclic amine-azachalcones compounds through molecular hybridization of chalcone scaffold and fragments of isoniazid, fluoroquinolones, and linezolid with antituberculosis potential. The new compounds were synthesized via Claisen-Schmidt condensation, providing yields of 36-95%. Fifteen compounds showed antituberculosis activity against Mycobacterium tuberculosis H37Rv strain. Two amine-azachalcones 15 and 17 showed relevant biological activity with minimum inhibitory concentration (MIC) values of 6.62 and 4.85 µM, respectively. Compound 12 showed the best profile of antitubercular activity with MIC = 9.54 µM and selectivity index (SI) = 9.33. It was found that morpholine group is important to increase potency of antimycobacterial activity but also to add some toxicity to the chalcone molecular framework. The results described herein would be a guide in the designing of novel and optimized antitubercular derivatives based on the chalcone scaffold.

https://doi.org/10.21577/0103-5053.20200013 417 downloads

PHYTOCHEMICAL AND BIOLOGICAL STUDIES OF CONSTITUENTS FROM ROOTS OF Salacia crassifolia (CELASTRACEAE)
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Santos, Josana Pereira dos

; Oliveira, Willian Xerxes Coelho

; Vieira-Filho, Sidney A.

; Pereira, Rafael C. G.

; Souza, Grasiely Faria de

; Gouveia, Viviane Alves

; Sabino, Adriano de Paula

; Evangelista, Fernanda C. G.

; Takahashi, Jacqueline Aparecida

; Moura, Marília A. F.

; Almeida, Filipe B.

; Duarte, Lucienir Pains

Química Nova

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may 2020, Volume 43 N. 5 Pages 558 - 567

Abstract: En | Text: En | PDF: En

Salacia crassifolia traditionally known as “Bacupari-do-Cerrado” is used to treat kidney problems, and as a healing agent for coughs and malaria. The phytochemical study of the S. crassifolia roots led to the isolation of thirteen compounds: abruslactone-A (1), urs-12-ene-3β,25,30-triol (2), carioprystimerin (3), β-sitosterol (4), pristimerin (5), dispermoquinone (6), netzahualcoyonol (7), 20-hydroxy-20-epi-tingenone (8), 6-oxo-pristimerol (9), 9β,10β-epoxi-3β-hydroxy-1βH,4βH,5βH,7βH,11αH-guaian-12,8β-olide (10), 3-O-b-D-glucosyl-b-sitosterol (11), 4`-O-methylepigalocatechin (12) and cerebroside (13). The chemical structures of 1-13 were determined by IR, 1D/2D NMR together with X-ray diffractometry. Compounds 2 and 10 are herein described for the first time. Extracts of S. crassifolia and compounds 3, 5, 8 and 9 were evaluated on acetylcholinesterase inhibition, in vitro cytotoxic activity and in vivo toxicity tests using Caenorhabditis elegans model. All tested compounds inhibited acetylcholinesterase, and compounds 3, 8 and 9 demonstrated a greater potential when compared to the standard eserine. The tested compounds showed low cytotoxicity against the THP-1, K562 and MDA-MB-231 cancer cell lines. None of the tested compounds and extracts were toxic against C. elegans since the larvae survival rate in L1 stage was higher than 90%.

https://doi.org/10.21577/0100-4042.20170520 329 downloads

On-farm welfare assessment in meat goat does raised in semi-intensive and extensive systems in semiarid regions of Ceará, Northeast, Brazil
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Leite, Luana Oliveira

; Stamm, Fabiana de Orte

; Maceno, Marcelo Adriano Corrêa

; Camarinha Filho, Jomar Antonio

; Garcia, Rita de Cassia Maria

Ciência Rural

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2020, Volume 50 N. 10 elocation e20190745

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O objetivo deste estudo foi aplicar um protocolo baseado nos protocolos Animal Welfare Indicators (AWIN) de pequenos ruminantes em fazendas do nordeste brasileiro para avaliar o bem-estar de cabras de corte, comparando animais mantidos em fazendas semi-intensivas (S) e extensivas (E). Quinze fazendas localizadas na Região Metropolitana de Quixadá e Quixeramobim, no Ceará, Nordeste do Brasil, foram recrutadas. As avaliações foram realizadas no nível de grupo e individual. A diferença significativa entre as fazendas S e E foi estabelecida em P<0,05 para todos os testes. No nível de grupo, houve diferença significativa, com mais cabras do grupo E afetadas por estresse térmico e do grupo S apresentando apatia. Indicadores baseados em recursos, tipo de bebedores e acesso a abrigos diferiram significativamente entre os sistemas S e E, com cabras em fazendas S alocadas em locais com acesso à água durante o período noturno mais frequentemente e protegidas do vento. O tipo de instalação era mais complexo para os animais nas fazendas S (n=2) do que nas fazendas E, devido as divisões internas, com a presença de comedouros, bebedouros, saleiros, piso ripado ou cimentado em fazendas S. A nível individual, abscesso e secreção ocular foram observados mais frequentemente nas fazendas S, e lesões nas orelhas, nas fazendas E, provavelmente devido ao manejo distinto das cabras. Semelhanças entre os resultados mostraram que os fazendeiros de ambos os sistemas de produção enfrentam problemas relacionados ao estresse térmico, falta de forragem e problemas de saúde.

ABSTRACT: The aim of this study was to apply a protocol based on the Animal Welfare Indicators (AWIN) protocols for small ruminants in Brazilian Northeast farms to evaluate the welfare of meat goat, comparing animals kept on semi-intensive (S) and extensive (E) farms. Fifteen farms located in the Metropolitan Region of Quixadá and Quixeramobim, Ceará, Brazilian Northeast, were recruited. Assessments were performed at group and individual level. Significant differences between S and E farms was set at P<0.05 to all tests. At group level, there was a significant difference between groups, with more goats from E farms affected by heat stress and more animals from S farms presenting oblivion. Resource-based indicators, type of drinkers and access to shelter, differed significantly between S and E systems, with goats on S farms allocated in places with access to water more frequently during night period and protected from wind. Type of facility was more complex for animals on S farms (n=2) than on all E farms due to internal divisions, with S farms presenting feeders, drinking fountains, salt shakers, and slatted or cemented floor. At individual level, abscess and ocular discharge were more frequently observed on S farms and ears lesions were more frequent on E farms, probably due to distinct management of goats. Similarities between results showed that farmers in both production systems faced problems related to heat stress, lack of forage and health issues.

https://doi.org/10.1590/0103-8478cr20190745 895 downloads

10.

Decolorization of textile azo dye Reactive Red 239 by the novel strain Shewanella xiamenensis G5-03 isolated from contaminated soil
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Cossolin, Aline Silva

; Reis, Hélen Cristina Oliveira dos

; Castro, Ketinny Camargo de

; Santos, Bruna Assis Paim dos

; Marques, Matheus Zimermann

; Parizotto, Carlos Adriano

; Dall´Oglio, Evandro Luiz

; Vasconcelos, Leonardo Gomes de

; Morais, Eduardo Beraldo de

Revista Ambiente & Água

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2019, Volume 14 N. 6 elocation e2446

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Shewanella xiamenensis G5-03 isolada de solo contaminado de aterro sanitário foi eficiente em descolorir cinco corantes têxteis azos em condições estáticas. Um deles, Reactive Red 239 (RR239), foi completamente descolorido na faixa de pH de 7 a 11 (a 35°C) dentro de 3-6 h. A bactéria também foi eficiente em descolorir RR239 em uma ampla faixa de temperatura de 25-40°C (em pH 8). A cinética de descoloração do RR239 pela G5-03 se ajustou ao modelo de Michaelis-Menten (K m = 443,3 mg L-1, V max = 166,7 mg L-1 h-1). A descoloração do RR239 foi monitorada por espectroscopia UV-Vis e FTIR, que mostrou mudanças significativas nas posições dos picos quando comparadas ao espectro do corante. No geral, a capacidade de S. xiamenensis G5-03 para descolorir os corantes têxteis azo em uma ampla faixa de temperatura e pH (neutro-alcalino) indica que esta cepa é uma candidata em potencial para o tratamento de efluente contendo corante.

Abstract Shewanella xiamenensis G5-03 isolated from contaminated landfill soil efficiently decolorized five textile azo dyes under static conditions. One of them, Reactive Red 239 (RR239), was completely decolorized at a pH range of 7 to 11 (at 35°C) within 3-6 h. The bacterium was also efficiently decolorized RR239 in a wide temperature range of 25-40°C (at pH 8). The kinetics of RR239 decolorization by G5-03 fitted to the Michaelis-Menten Model (K m = 443.3 mg L-1, V max = 166.7 mg L-1 h-1). The decolorization of RR239 was monitored by UV-Vis and FTIR spectroscopy, which showed significant changes in peak positions when compared to the dye spectrum. Overall, the ability of S. xiamenensis G5-03 to decolorize textile azo dyes in a wide range of temperatures and pH (neutral-alkaline) indicate that this strain is a potential candidate for treating dye-containing effluent.

https://doi.org/10.4136/ambi-agua.2446 1033 downloads

11.

Inoculação e aplicação de diferentes doses de nitrogénio na cultura do feijoeiro
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Florentino, Ligiane A.

; F. Junior, Kleso S.

; P. Filho, Mario V.

; Oliveira, Tayla Évellin de

; Souza, Flávia R. C.

; Silva, Adriano B.

Revista de Ciências Agrárias

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dec 2018, Volume 41 N. 4 Pages 91 - 100

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Este estudo teve como objectivo avaliar o desenvolvimento e produtividade da cultura do feijão (Phaseolus vulgaris L.), quando submetida à inoculação e aplicação de fertilizante nitrogenado (N) à sementeira e/ou cobertura. O delineamento experimental foi em blocos casualizados (DBC), em esquema fatorial 4 (N sementeira, N cobertura, N sementeira e cobertura e sem aplicação de N) x 2 (sem e com inoculação da estirpe CIAT 899T), com três repetições. Aos 28 dias após a sementeira, todos os tratamentos receberam aplicação via foliar de molibdénio na dose de 80,0 g ha-1. Nesse mesmo período, os tratamentos contendo N cobertura receberam aplicação de 40 kg ha-1. Na época de floração foram avaliados os parâmetros de matéria seca da parte aérea (MSPA), teor de nitrogénio da parte aérea (TNPA), número de nódulos (NN) e matéria seca dos nódulos (MSN). Aos 120 dias (época da colheita), foram avaliados o número de vagens (NVP) e de grãos por planta (NGP) e produtividade de grãos (PG - kg ha-1). A inoculação mostrou-se uma prática viável quando não foi utilizado nitrogénio mineral e o N foi utilizado somente na cobertura, sendo capaz de promover bom desenvolvimento vegetal e produtividade, no entanto, não equiparável à dose completa de N mineral.

The objective of this research was to evaluate the development and productivity of bean crop (Phaseolus vulgaris L.), when submitted to the inoculation and application of nitrogen fertilizer (N) at sowing and/or top dressing (cover). The experimental design was in randomized blocks (RBD) in 4 (N sowing, N top dressing, N sowing and top dressing and without N application) x 2 (with and without inoculation of strain CIAT 899T) factorial combination with three replications. At 28 days after sowing, all treatments received the foliar application of 80.0 g ha-1of molybdenum. In the same period, the treatments containing N top dressing received the application of 40 kg ha-1. At flowering time, the parameters, dry matter of the aerial part (DMAP), shoot nitrogen content (TNPA), number (NN) and dry matter of nodules (MSN) were evaluated. After 120 days (harvest time) the following parameters were evaluated: number of pods (NVP) and grains per plant (NGP) and grain yield (PG - kg ha-1). Inoculation proved to be a viable practice when it was not used mineral nitrogen. The use of inoculated seeds and also top dressing of nitrogen fertilization in cover promoted good development and productivity, however, not comparable to the full dose of mineral N.

https://doi.org/10.19084/RCA17001 795 downloads

12.

Surto de esporotricose felina na região metropolitana do Recife
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Silva, Grasiene M.

; Howes, Julio Cesar F.

; Leal, Carlos Adriano S.

; Mesquita, Emanuela P.

; Pedrosa, Camila M.

; Oliveira, Andréia Alice F.

; Silva, Leonildo Bento G.

; Mota, Rinaldo A.

Pesquisa Veterinária Brasileira

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sep 2018, Volume 38 N. 9 Pages 1767 - 1771

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: A esporotricose é uma micose subcutânea, causada por um fungo dimórfico do complexo Sporothrix schenckii que acomete várias espécies, especialmente a felina, causando comprometimento local, disseminado ou sistêmico. Objetivou-se relatar a ocorrência de um surto de esporotricose felina na Região Metropolitana do Recife. Foram coletados suabes de úlceras cutâneas de felinos atendidos no Hospital Veterinário da Universidade Federal Rural de Pernambuco e em Clínicas Veterinárias particulares. O diagnóstico foi realizado por meio de exame citológico e cultura fúngica. Dos 115 casos suspeitos, 59 casos foram confirmados, número nunca registrado anteriormente no estado de Pernambuco. Os casos de esporotricose felina registrados têm demonstrado aspectos clínicos e epidemiológicos semelhantes aos ocorridos em outras regiões do país, caracterizando-se como uma doença predominantemente de machos jovens em idade reprodutiva e semi-domiciliados, de difícil tratamento e de rápida disseminação.

ABSTRACT: Sporotrichosis is a subcutaneous mycosis caused by a dimorphic fungus from Sporothrix schenckii complex, which affects various species, especially feline, able to cause local, disseminated or systemic impairment. We aimed to report the occurrence of an outbreak of feline sporotrichosis in the Metropolitan Region of Recife. Swabs of skin ulcers in cats treated in the Veterinary Hospital of the Federal Rural University of Pernambuco and in particular veterinary establishments were collected. Diagnosis was made by cytological examination and fungal culture. From 115 suspicious cases, 59 cases were confirmed, unprecedented results in the state of Pernambuco. These registered cases of feline sporotrichosis have demonstrated clinical and epidemiological characteristics similar to those occurring in other regions of the country, characterized as a quick-spreading disease, of difficult treatment, which affects predominantly semi-domiciled young males in reproductive age.

https://doi.org/10.1590/1678-5150-pvb-5027 7345 downloads

13.

A Validated NMR Approach for MDMA Quantification in Ecstasy Tablets
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Almeida, Nathalia S.

; Benedito, Luiz E. C.

; Maldaner, Adriano O.

; Oliveira, Aline L. de

Journal of the Brazilian Chemical Society

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sep 2018, Volume 29 N. 9 Pages 1944 - 1950

Abstract: En | Text: En | PDF: En

In recent years, ecstasy trafficking has grown in complexity and tablets has been commonly sold containing different contents of 3,4-methylenedioxymethamphetamine (MDMA) or even other psychoactive substances. In contrast, identification and quantification of drugs of abuse is still a challenge, once the access to drug standards is very restricted in Brazil. In this work, ecstasy tablets seized by the Brazilian Federal Police have their chemical composition identified and quantified by gas chromatography and quantitative 1H nuclear magnetic resonance based on an internal standard approach (IS-1H-qNMR). Both methods were validated and showed suitable results for the figures of merit. IS-1H-qNMR shows excellent results of accuracy (relative error < 5%) and precision (relative standard deviation (RSD) < 2%). Comparatively, IS-1H-qNMR is more efficient and versatile than gas chromatography to accomplish in a single analysis the identification and quantification of target analytes. Since the method does not require a specific reference material, it is cost effective and provides agility to routine forensic analysis.

https://doi.org/10.21577/0103-5053.20180071 2165 downloads

14.

ELECTRICITY GENERATION FROM BIOGAS ON SWINE FARM CONSIDERING THE REGULATION OF DISTRIBUTED ENERGY GENERATION IN BRAZIL: A CASE STUDY FOR MINAS GERAIS
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Antônio, Adriano da S.

; Oliveira Filho, Delly

; Silva, Samuel C. da

Engenharia Agrícola

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aug 2018, Volume 38 N. 4 Pages 518 - 525

Abstract: En | Text: En | PDF: En

ABSTRACT The objective of this study was to analyze the feasibility of using agricultural waste from a swine farm to produce biogas, which can be used to generate electricity. For this purpose, the waste production potential was evaluated to determine the biogas production capacity of the farm. This measurement allowed scaling the size of the generator used to the electricity production to meet the needs of the farm as well as surplus electricity. The surplus electricity may be used on the farm when the generator is under maintenance or the electricity consumption is larger than the energy generated. This process is regulated by Normative Resolutions 482 and 687 in Brazil. The results of the analysis of the net present value, internal return rate, payback period and benefit cost ratio indicated that the project was feasible.

https://doi.org/10.1590/1809-4430-eng.agric.v38n4p518-525/2018 2167 downloads

15.

Brazilian legislation on genetic heritage harms Biodiversity Convention goals and threatens basic biology research and education
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ALVES, RUY JOSÉ V.

; WEKSLER, MARCELO

; OLIVEIRA, JOÃO A.

; BUCKUP, PAULO A.

; POMBAL JR., JOSÉ P.

; SANTANA, HÉLCIO R.G.

; PERACCHI, ADRIANO LÚCIO

; KELLNER, ALEXANDER W.A.

; ALEIXO, ALEXANDRE

; LANGGUTH, ALFREDO

; ALMEIDA, ALZIRA MARIA P. DE

; ALBERNAZ, ANA LUISA

; RIBAS, CAMILA C.

; ZILBERBERG, CARLA

; GRELLE, CARLOS EDUARDO V.

; ROCHA, CARLOS F.D.

; LAMAS, CARLOS JOSÉ E.

; HADDAD, CÉLIO F.B.

; BONVICINO, CIBELE R.

; PRADO, CYNTHIA P.A.

; LIMA, DANIELA O. DE

; ROSSA-FERES, DENISE C.

; SANTOS, FABRÍCIO R. DOS

; SALIMENA, FÁTIMA REGINA G.

; PERINI, FERNANDO A.

; BOCKMANN, FLÁVIO A.

; FRANCO, FRANCISCO LUÍS

; GIUDICE, GISELE M.L. DEL

; COLLI, GUARINO R.

; VIEIRA, IMA CÉLIA G.

; MARINHO-FILHO, JADER

; WERNECK, JANE M.C.F.

; SANTOS, JORGE A.D. DOS

; NASCIMENTO, JORGE LUIZ DO

; NESSIMIAN, JORGE LUIZ

; CORDEIRO, JOSÉ LUIS P.

; CLARO, KLEBER DEL

; SALLES, LEANDRO O.

; CASATTI, LILIAN

; PY-DANIEL, LUCIA HELENA R.

; SILVEIRA, LUÍS FÁBIO

; TOLEDO, LUÍS FELIPE

; OLIVEIRA, LUIZ F. DE

; MALABARBA, LUIZ ROBERTO

; SILVA, MARCELO D. DA

; COURI, MÁRCIA S.

; MARTINS, MÁRCIO

; TAVARES, MARCOS D.S.

; SOBRAL, MARCOS EDUARDO G.

; VIEIRA, MARCUS VINÍCIUS

; OLIVEIRA, MARIA DE LOURDES A.

; PINNA, MÁRIO DE

; HOPKINS, MICHAEL J.G.

; SOLÉ, MIRCO

; MENEZES, NAÉRCIO A.

; PASSOS, PAULO

; D’ANDREA, PAULO SERGIO

; PINTO, PEDRO C.E.A.

; VIANA, PEDRO L.

; TOLEDO, PETER M.

; REIS, ROBERTO E.

; VILELA, ROBERTO

; BASTOS, ROGÉRIO P.

; COLLEVATTI, ROSANE G.

; CERQUEIRA, RUI

; CASTROVIEJO-FISHER, SANTIAGO

; CARAMASCHI, ULISSES

Anais da Academia Brasileira de Ciências

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apr 2018, Volume 90 N. 2 Pages 1279 - 1284

Abstract: En | Text: En | PDF: En

https://doi.org/10.1590/0001-3765201820180460 7311 downloads

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