Resumo Objetivou-se avaliar o perfil metabólico de 24 ovinos Santa Inês, machos, com peso vivo médio inicial de 20±1,25 kg, submetidos à restrição alimentar seguida de realimentação em confinamento, distribuídos em quatro tratamentos com 0, 20, 40 ou 60% de restrição alimentar. Dois ensaios foram desenvolvidos, um referente à restrição e outro à realimentação. Para tanto, foi utilizado delineamento experimental inteiramente ao acaso, com quatro tratamentos e seis repetições. Observou-se na fase de restrição alimentar que houve um decréscimo linear para as concentrações bioquímicas de ureia, glicose, GGT e fósforo; contrariamente, a concentração de colesterol aumentou linearmente ao final do período restritivo. A restrição alimentar influenciou negativamente nas concentrações de triglicerídeos e creatinina, diferentemente, os valores de proteínas totais e albumina apresentaram aumento significativo com o período restritivo. Já na realimentação, observou-se comportamento linear decrescente para ureia, glicose, GGT e magnésio. No segundo ensaio, verificou-se que as concentrações de proteínas totais, globulina e creatinina apresentaram diferença (P<0,05) entre os períodos de coleta. Os níveis de restrição alimentar afetaram o metabolismo proteico e interferiram também no metabolismo energético e mineral e a realimentação resultou em alteração no metabolismo proteico e energético.

Abstract The objective of this study was to evaluate the metabolic profile of 24 male Santa Ines sheep, with initial mean live weight of 20 ± 1.25 kg, submitted to feed restriction followed by refeeding, distributed into four treatments with 0, 20, 40 or 60 % of food restriction. Two trials were carried out, one concerning restriction and the other one for feedback. For this, a completely randomized experimental design was used, with four treatments and six replicates. It was observed in the food restriction phase that there was a linear decrease in biochemical concentrations of urea, glucose, GGT, and phosphorus; conversely, the cholesterol concentration increased linearly at the end of the restrictive period. Food restriction had a negative effect on triglyceride and creatinine concentrations. Differently, total protein and albumin values increased significantly with the restriction period. In the refeeding, a linear decreasing behavior was observed for urea, glucose, GGT and magnesium. In the second assay, total protein, globulin and creatinine concentrations were found to differ (P <0.05) between the collection periods. Food restriction levels affected protein metabolism and also interfered with energy and mineral metabolism, and refeeding resulted in altered protein and energy metabolism.

The objective of this experiment was to estimate the curve of dehydration, chemical composition and in vitro dry matter digestibility (IVDMD) and crude protein digestibility (IVCPD) of tifton 85 hay, produced single or overseeded with ryegrass or white oat IPR 126. The experimental design was in randomized blocks, in a 3 × 10 factorial arrangement, with three cropping systems and ten evaluation times (0, 4, 8, 24, 28, 32, 48, 52 and 56 and 71 hours after harvest) during the dehydration process. For chemical composition and in vitro DM and CP digestibility, the experimental design was in randomized blocks with three cropping systems and three evaluation times (before cutting, before baling and 100 days after of storage). It was found that tifton 85 intercropped with white oat kept its higher nutritional value after storage, with 19.78% crude protein and 70.03% of in vitro dry matter digestibility. The participation of white oat in tifton 85 area was 57.04% and for ryegrass, it was 38.52%, but the dry matter yield of single tifton 85 was higher than other intercrops. Thus, it is recommended an oversowing of winter annual species on Tifton 85, without moisture restriction, because of the benefits obtained in the hay nutritional value.

Com o objetivo de avaliar o efeito do uso de probiótico (Saccharomyces cerevisiae) em dietas para cavalos, utilizaram-se quatro equinos machos com média de 400 kg de peso corporal, em delineamento em quadrado latino 4 õ 4. As dietas foram compostas de concentrado e feno de capim-tifton 85 (de baixa qualidade) ou feno de alfafa (de alta qualidade), com ou sem a adição de levedura. Cada período experimental teve duração de 36 dias, com coleta total de fezes, para determinação dos coeficientes de digestibilidade aparente dos nutrientes, e coleta de fezes, para determinação do pH e da microbiologia fecal. A utilização de probiótico não alterou os coeficientes de digestibilidade aparente dos nutrientes das dietas, com exceção do extrato etéreo. A qualidade nutricional do feno influenciou os coeficientes de digestibilidade da matéria seca (MS), da proteína bruta (PB) e do extrato etéreo (EE) apenas no caso das dietas com feno de capim-tifton sem probiótico e com feno de alfafa com probiótico. A utilização de cultura de levedura aumentou a população de Lactobacillus nas fezes dos animais que receberam a dieta com feno de capim-tifton com probiótico. Não houve uso de probiótico na população de Lactobacillus nas dietas com feno de alfafa (de boa qualidade). A população de Streptococcus nas fezes aumentou com suplementação de levedura, tanto na dieta com feno de capim-tifton como naquela com feno de alfafa. A dieta com capim-tifton 85 sem probiótico promoveu redução nos valores de pH fecal 14 e 17 horas após a alimentação. Considerando ainda os valores de pH fecal nesses períodos após a alimentação, as dietas suplementadas com probiótico apresentaram valores superiores àqueles obtidos sem adição de levedura.

In order to evaluate the effect of using probiotic (Saccharomyces cerevisiae) in diets for horses, it was used four male equines, with average body weight of 400 kg, in a 4 õ 4 Latin square design. Diets were composed of concentrated and 85 Tifton grass hay (low quality) or alfafa hay (high quality), with or without addition of yeast. Each experimental period lasted 36 days, with total fecal collection for determination of apparent digestibility coefficients of the nutrients and fecal collection for determining pH and fecal microbiology. Use of probiotic did not affect apparent digestibility coefficients of the diets, except for ether extract. Hay nutritional quality affected coefficients of dry matter digestibility, crude protein and ether extract (EE) only for diets with Tifton grass hay without probiotic and with alfafa hay with probiotic. The utilization of yeast culture increased the Lactobacillus population in feces of animals fed diet with Tifton grass hay with probiotic. Probiotic was not used in population of Lactobacillus in diets of alfafa hay (good quality). Streptococcus population in the diet increased as yeast was supplemented in both diets with Tifton grass hay as that with alfafa hay. Diet with 85 Tifton grass without probiotic promoted reduction in fecal pH values 14 and 17 hours after feeding. Considering the values of fecal pH in this periods after feeding, the diets supplemented with probiotic showed values higher than those without addition of yeast.