Abstract Doxorubicin (Dox) is a medication used in the treatment of cancerous tumors and hematologic malignancies with potentially serious side effects, including the risk of cardiotoxicity. Flavonoids are plant metabolites with antioxidant properties and can be extracted from Camellia sinensis (CS). The aim of this study is to evaluate the possible cardioprotective effect of CS against injuries induced by Dox in rats. A total of 32 animals were distributed into four groups: (1) control - intraperitoneal injection (I.P.) of 0.5 mL saline weekly and 1.0 mL water by gavage daily; (2) CS - 0.5 mL saline I.P. weekly and 200 mg/kg CS by gavage daily; (3) Dox - 5.0 mg/kg Dox I.P. weekly and 1.0 mL water by gavage daily; and (4) Dox+CS -5.0 mg/kg Dox I.P. weekly and 200 mg/kg CS by gavage daily. Clinical examinations, blood profiles, electrocardiograms, echocardiograms, and histological analyses of hearts were performed over 25 days. The animals in the Dox group showed changes in body weight and in erythrogram, leukogram, electrocardiography, and echocardiography readings. However, animals from the dox+CS group had significantly less change in body weight, improved cardiac function, and showed more preserved cardiac tissue. This study demonstrated that CS prevents dox-induced cardiotoxicity, despite enhancing the cytotoxic effect on blood cells.

ABSTRACT Purpose: To evaluate the long-chain fatty acid and major compounds levels in the feces after prophylactic oral use of Lacticaseibacillus casei in an experimental model of intestinal mucositis. Methods: Fifteen Swiss mice were randomly divided into three groups (n=5/group): The negative or positive control groups (n = 5) received saline orally for 18 days and an the intraperitoneal (i.p.) of saline or 5 Fluorouracil (450 mg/kg) in 15th day, respectability. L. casei group received oral concentration of L. casei (1x109 CFU/mL) for 18 days, the i.p. injection of 5-fluorouracil (450 mg/kg) in 15th days. Tissue samples from colon and each small intestine segment were collected for histopathological analysis. Stool samples were collected. Fecal composition of long-chain fatty acids and sterols were analysed by gas chromatography-mass spectrometry on the 15th and the 18th day. Results: The mucosa layer of all small intestine segments of animals from L. casei showed well preserved epithelium and glands, without necrosis signs, but Goblet cells number decreased. Several long-chain fatty acids and sterols have been identified before and after in the groups. L. casei administration after 5-FU treatment reduced concentrations of linoleic acid (18:2) (p < 0.001) and oleic acid (18:1) (p < 0.001) in feces. Conclusions: L. casei prevented the mucosal damage associated with 5-FU-induced intestinal mucositis reduced long-chain fatty acid levels in the feces.

ABSTRACT The present study aimed to assess the precision of oxygen uptake with heart rate regression during treadmill running in highly trained runners. Twenty national and international level male runners were divided into two equal groups. Group A was formed of 10 sprinters (31.5± 4.7 years, height 1.74± 0.04 m and mass 61.8± 5.2 kg), and group B comprised 10 endurance runners (25.7± 4.5 years, height 1.77± 0.08 m and mass 71.2± 5.8 kg). Each participant performed six min bouts at a constant velocity on a level treadmill, with each bout at an increased running velocity. The starting velocity was 3.33 m·s-1, with a 0.56 m·s-1 increase in each subsequent bout. VO2 and HR were measured during all runs. Mean peak VO2 and mean peak HR for Group A were, respectively, 71.7± 7.0 mL·kg-1·min-1 and 179.8± 12.7 beats·min-1, while for Group B were 67.3± 4.9 mL·kg-1·min-1 and 198.2± 9.8 beats·min-1. The linearity of the regressions between VO2 and HR in both groups was very high (R2= 0.97 and R2=0.76) with small standard errors of regression. Despite a stronger correlation in endurance-trained athletes, the results of the present study indicate that it is possible to use HR as an indicator of exercise intensity in individuals trained in both speed and longer distances.

Abstract: New communication technologies have brought significant corporate organisation and communication changes. In this study, we present some of these transformations, particularly focusing on: the need for organisations to (re)establish symbolic connections within an environment with no physical presence and a reduced ability to create, exchange and maintain the organisational culture and identity. Thus, companies increasingly invest in new practices in social media to stand out in an environment loaded with images and noise where everything seems ephemeral and disjointed. Against the background of a fragmented society, we draw an analysis of the strategic communication of the Virtual Museum of Lusophony during its launch on Instagram. The research demonstrates the cultural organisation joined the social media essentially because it understood it was essential to achieve its purposes and, for coherence and consistency with the strategic communication, anchored by: (a) actions to create a visual pattern that consolidates the organisational identity; (b) a clear editorial line to publish images adding value to aspects of the organisation; and (c) practices implemented using the platform's interactive resources, towards an immediate increase in the engagement with the museum's audience and potential audience.

Resumo: O fenómeno das novas tecnologias da comunicação trouxe mudanças significativas nas formas de organização e comunicação nas empresas. Neste estudo, apresentamos algumas destas transformações, sendo que uma delas nos chama a atenção: as organizações precisam de (re)estabelecer conexões simbólicas dentro de um ambiente em que não há presença física e em que se reduz a capacidade de criação, troca e manutenção da cultura e da identidade organizacionais. Nesse sentido, as empresas investem cada vez mais em novas práticas nos média sociais, para sobressair num ambiente carregado de imagens, barulhento, em que tudo parece efémero, desarticulado. Diante do contexto de uma sociedade fragmentada, trazemos o estudo de caso da comunicação estratégica do Museu Virtual da Lusofonia durante o seu lançamento no Instagram. Na pesquisa, observa-se que a entrada da organização cultural neste medium social foi fundamentado essencialmente pela compreensão da organização e dos seus propósitos e, ao mesmo tempo, pela coerência e consistência da comunicação estratégica, ancorados por: (a) ações de criação de um padrão visual que reforça a identidade organizacional; (b) uma linha editorial clara para publicação de imagens que valoriza aspetos da organização; e (c) práticas realizadas a partir de recursos interativos da plataforma, levando a um incremento inicial imediato do relacionamento com os públicos reais e potenciais do museu.

Resumen Introducción. La ciclofosfamida se usa para tratar neoplasias malignas y controlar enfermedades autoinmunitarias, pero uno de sus metabolitos, la acroleína, es tóxico para el urotelio y puede provocar cistitis hemorrágica y malestar grave. Objetivo. Evaluar la capacidad del propóleos rojo para prevenir y tratar la cistitis hemorrágica inducida por ciclofosfamida en ratas. Materiales y métodos. Se extrajo propóleos rojo en goma arábiga al 1 % y se administró por vía subcutánea. En el primer experimento, los grupos IA, IIA, IIIA, IB, IIB y IIIB recibieron agua, goma arábiga y propóleos, respectivamente, durante 30 días. Luego se les administró agua (controles) o el tratamiento (ciclofosfamida) por inyección intraperitoneal. En el segundo experimento, los grupos IVA, VA, VIA recibieron agua por vía intraperitoneal, y los grupos IVB, VB, VIB recibieron el tratamiento por la misma vía, a lo que le siguieron cinco inyecciones con intervalos de dos horas entre ellas, con agua, goma arábiga o propóleos. El tejido de la vejiga se examinó de acuerdo con los criterios de Gray. Resultados. La puntuación total de la inflamación según la histología fue significativamente menor en el grupo VIB (11,33 ± 2,07). La inflamación leve predominó en este grupo, en tanto que la mayoría de los animales del IVB presentó inflamación grave (p=0,0375). Predominaron las úlceras múltiples en los grupos IVA y VB, pero fueron raras o estuvieron ausentes en el VIB (p=0,0118). En general, no se observaron células uroteliales en los grupos IVB y VB, pero sí en el VIB (p=0,0052). La fibrina fue abundante en los grupos IVB y VA, pero predominantemente ausente en el VIB (p=0,0273). Conclusiones. El propóleos rojo puede reducir la inflamación en la cistitis hemorrágica inducida por ciclofosfamida en ratas.

Abstract Introduction. Cyclophosphamide (CP) is used to treat malignant neoplasias and control autoimmune diseases. Still, one of its metabolites, acrolein, is toxic to the urothelium and can lead to hemorrhagic cystitis and severe discomfort. Objective. To evaluate the ability of red propolis to prevent and treat CP-induced hemorrhagic cystitis in rats. Materials and methods. Red propolis was extracted in 1% gum arabic and administered subcutaneously (sc). In the first experiment, groups IA, IIA, and IIIA and groups IB, IIB, and IIIB received water, gum arabic (GA), or propolis, respectively, for 30 days. Then water (controls) or CP (treatment) was administered i.p. In the second experiment, groups IVA, VA, and VIA received water i.p. while groups IVB, VB, and VIB received CP i.p. This was followed by 5 injections at 2-hour intervals with either water, GA, or propolis. Bladder tissue was examined according to Gray’s criteria. Results. The total inflammatory histology score was significantly smaller in group VIB (11.33 ± 2.07). Mild inflammation predominated in group VIB while most of the animals in group IVB had severe inflammation (p=0.0375). Ulcers were predominantly multiple in Groups IVA and VB but rare or absent in Group VIB (p=0.0118). Urothelial cells were mostly absent in groups IVB and VB and present/normal in group VIB (p=0.0052). Fibrin was abundant in groups IVB and VA but mostly absent in group VIB (p=0.0273). Conclusions. Red propolis can reduce inflammation in CP-induced hemorrhagic cystitis in rats.

RESUMO O envelhecimento está associado a patologias crónicas, que podem ser prevenidas ou retardadas através do envolvimento em comportamentos saudáveis, como a prática regular de Exercício Físico (EF). O objetivo deste trabalho foi caracterizar a qualidade de vida, vitalidade e força de preensão manual de idosos participantes em programas comunitários de EF, bem como analisar a relação da prática de EF com estas variáveis. Recorreu-se a uma amostra composta por 81 indivíduos, 49 do sexo feminino e 32 do sexo masculino, com idades compreendidas entre os 65 e os 85 anos (72.33±5.02). Os resultados indicam-nos que os elementos do género masculino apresentavam valores superiores nas variáveis força de preensão manual (p<0.001; η2=0.510), vitalidade subjetiva (p=0.05; η2=0.005) e no domínio “intimidade” da qualidade de vida (p=0.01; η2=0.005). Relativamente à análise das relações entre as variáveis, no género feminino a força de preensão manual está relacionada com a frequência semanal (r=0.42; p=0.003), com o número de horas de prática (r=0.49; p<0.001), e com a qualidade de vida geral (r=0.35; p=0.015). Ainda no género feminino, verificamos a existência de uma regressão linear significativa da frequência de prática semanal (R2 ajustado=0.23; p<0.001) e do número de horas semanal (R2 ajustado=0.30; p<0.001), com a força de preensão manual. Já no género masculino, a força de preensão manual correlaciona-se com o número de horas de prática semanal (r=0.38; p=0.033). Já a vitalidade está relacionada com a força de preensão manual (r=0.49; p=0.004) e com a qualidade de vida geral (r=0.61; p<0.001). Parece assim evidente o papel do EF por parte desta população, bem como a inclusão do treino de força uma vez que esta variável parece estar associada a uma melhor perceção de qualidade de vida e de vitalidade pelo idoso.

RESUMEN El envejecimiento se asocia con patologías crónicas, que pueden prevenirse o retrasarse al participar en comportamientos saludables, como el ejercicio físico regular (EF). Así, el objetivo de este estudio fue caracterizar la calidad de vida, vitalidad y fuerza de la empuñadura de los participantes de edad avanzada en programas comunitarios de EF, así como analizar la relación de la práctica con estas variables. Se utilizó una muestra de 81 individuos, 49 mujeres y 32 hombres, de entre 65 y 85 años (72.33±5.02). Los resultados indican que los elementos masculinos presentaron valores más altos en las variables fuerza de prensión manual (p<0.001; η2 =0.510), vitalidad subjetiva (p=0.05; η2=0.005) y en el dominio "η2=0.005). En cuanto al análisis de las relaciones, en las mujeres la fuerza de prensión manual está asociada con la frecuencia semanal (r=0.42; p=0.003), con el número de horas de práctica por semana (r=0.49; p<0.001), y con la calidad de vida general (r=0.35; p=0.015). También en las mujeres, verificamos una regresión lineal significativa de la frecuencia semanal (R2 ajustado =0.23; p<0.001) y el número de horas de práctica por semana (R2 ajustado= 0.30; p <0.001), con fuerza de prensión manual. En los hombres, verificamos que la fuerza de presión manual está relacionada con el número de horas de práctica semanal (r=0.38; p=0.033). La vitalidad está relacionada con la fuerza de prensión manual (r=0.49; p=0.004), y la calidad de vida general (r=0.61; p<0.001). Así, parece evidente el papel del EF por parte de esta población, así como la inclusión del entrenamiento de fuerza, ya que esta variable parece estar asociada a una mejor percepción de la calidad de vida y vitalidad por parte de los ancianos.

ABSTRACT Ageing is associated with chronic pathologies, which can be prevented or delayed through the involvement in healthy behaviours, such as regular physical exercise (PE). The aims of this study were to characterize the quality of life, vitality and handgrip strength of elderly people, participants in PE community programs, as well as to analyze the relationship of PE practice with these variables. A sample of 81 individuals, 49 females and 32 males, aged between 65 and 85 years (72.33 ± 5.02) were recruited. The results showed that men had higher values in the variables handgrip strength (p<0.001; 2 =0.510), subjective vitality (p=0.05; 2=0.005) and in the “intimacy” domain of quality of life (p=0.01; 2 =0.005). Regarding the analysis of the relationships, in females, handgrip strength is related with the weekly frequency (r=0.42; p=0.003), with the number of practice hours per week (r=0.49; p<0.001), and with general quality of life (r=0.35; p=0.015). Still in females, we verified the existence of a significant linear regression of the weekly frequency of practice (adjusted R2=0.23; p<0.001) and the number of weekly hours (adjusted R2=0.30; p<0.001), with handgrip strength. In males, we verified that handgrip strength is related with the number of hours of weekly practice (r=0.38; p=0.033). Finally, vitality is related with handgrip strength (r=0.49; p=0.004), and general quality of life (r=0.61; p<0.001). Thus, the role of PE practice in this population seems evident, not only for the associated physical, mental, and social benefits but also for the role it appears to play in the perception of quality of life and subjective vitality. Moreover, it still seems essential to include strength training, which appears to be associated with a better perception of quality of life and vitality by the elderly.

RESUMO O objetivo deste estudo foi analisar a evolução do Boccia de 1984 a 2016 nos Jogos Paralímpicos (JP) e vislumbrar futuras perspetivas na modalidade paralímpica. Dados foram obtidos da página oficial do Comité Paralímpico Internacional, de 1984 até 2016 e associados à classificação dos atletas, formato competitivo e número de jogos disputados, número, nacionalidade e género dos participantes, e também resultados. O número de atletas participantes nos JP aumentou constantemente entre 1984 (19) e 2016 (106), o mesmo foi observado relativamente aos países representados (1984 = 5 vs 2016 = 21) e países medalhados (1984 = 5 vs 2012 e 2016 = 11). No que respeita ao género, um aumento evidente foi observado, embora não contínuo ao longo das edições (masculino 1984 = 11 vs 2016 = 73 / feminino 1984 = 8 vs 2016 = 33). Apesar do número total de jogos ter aumentado entre 1984 (19) e 2016 (180), este não foi uniforme, tendo mesmo diminuído entre 2004 (252) e 2012 (161). Esta evidência está relacionada com fatores como modelo de qualificação e formato da competição, que mudaram ao longo do tempo, bem como sistema de classificação (BC4 implementada em 2004) e formato de pares e equipas. De realçar ainda que, na edição de 1984, os JP foram realizados com separação de géneros. O Boccia é uma modalidade paralímpica em desenvolvimento com vários desafios, nomeadamente: i) o aumento contínuo de participantes; ii) igualdade de género; iii) melhoria do sistema de classificação; e iv) formato competitivo para acomodar um número crescente de participantes.

RESUMEN El objetivo de este estudio fue analizar la evolución de la Bocha de 1984 a 2016 en los Juegos Paralímpicos (JP) y vislumbrar perspectivas futuras en el paradeporte. Los datos se obtuvieron de la página oficial del Comité Paralímpico Internacional, de 1984 a 2016 y asociados a la clasificación de atletas, formato competitivo y número de partidos disputados, número, nacionalidad y sexo de los participantes, así como resultados. El número de atletas que participaron en el JP aumentó de manera constante entre 1984 (19) y 2016 (106), lo mismo se observó para los países representados (1984 = 5 vs 2016 = 21) y países que ganan medallas (1984 = 5 vs 2012 y 2016 = 11). En cuanto al género, se observó un aumento evidente, aunque no continuo a lo largo de las ediciones (masculino 1984 = 11 vs 2016 = 73 / femenino 1984 = 8 vs 2016 = 33). A pesar de la cantidad total de juegos ha aumentado entre 1984 (19) y 2016 (180), este no fue uniforme, incluso disminuyó entre 2004 (252) y 2012 (161). Esta evidencia está relacionada con factores como el modelo de clasificación y el formato de la competición, que ha cambiado con el tiempo, así como el sistema de clasificación (BC4 implementado en 2004) y el formato de parejas y equipos. Cabe señalar también que, en la edición de 1984, los JP se realizaron con separación de género. La Bocha es un paradeporte en desarrollo con varios desafíos, a saber: i) el aumento continuo de participantes; ii) igualdad de género; iii) mejoría del sistema de clasificación; y iv) formato competitivo para acomodar a un número cada vez mayor de participantes.

ABSTRACT This study aimed to analyze the participation in Boccia from 1984 to 2016 in Paralympic Games (PG) and envision future perspectives in the parasport. Data was obtained from official International Paralympic Committee webpage, from the first edition (1984) until 2016 and associated to athlete's classification, competitive format and number of played games, the number, nationality and gender of participants, and also the results. The number of athletes participating in PG has steadily increased between 1984 (19) and 2016 (106), the same was observed with relation to represented countries (1984 = 5 vs 2016 = 21) and medal countries (1984 = 5 vs 2012 and 2016 = 11). With respect to athletes' gender, an evident increase was observed, although not continuous throughout the PG editions (male 1984 = 11 vs 2016 = 73 / female 1984 = 8 vs 2016 = 33). Despite the total number of played games increased between 1984 (19) and 2016 (180), this has not been uniform, having even decreased between 2004 (252) and 2012 (161). This evidence is related to factors such as forms of qualification and competition format, that have changed over time, as well as classification system (BC4 was implement in 2004), and pairs and teams' format. It should also be highlighted that, in 1984 edition, PG were performed separating genders. Boccia is a developing parasport with several challenges, namely: i) the continuous increase in participants; ii) gender equally; iii) improvement of classification system; and iv) the competitions format to accommodate an increasing number of participants.

Resumen Introducción: la disfagia es una complicación frecuente después del ictus, estando asociada a mayor riesgo de neumonía, deshidratación y desnutrición. Lo cribado reduce la incidencia de neumonía después del ictus. Objetivo: Traducir, adaptarse e probar la versión portuguesa de lo Gugging Swallowing Screen. Métodos: 226 pacientes con ictus fueron incluidos prospectivamente en un estudio multicêntrico e las enfermeras aplicaron lo Gugging Swallowing Screen cuando el paciente ingresó. Los resultados se compararon con las puntuaciones de la National Institute Health Stroke Scale, la Escala de Coma de Glasgow y el juicio clínico de la enfermera. Se calculó la confiabilidad entre evaluadores. Resultados: La incidencia de disfagia fue del 47.3%. La sensibilidad, especificidad y área bajo la curva característica operativa del receptor fueron 85.4%, 84.6% y 0.892, respectivamente, utilizando el juicio clínico como referencia. Se estableció asociación estadísticamente significativa entre cribado positivo y mayor déficit neurológico (χ2 = 32.99; p <0.001) y ausencia de disfagia con coma leve (χ2 = 15.15; p <0.001). Se logró excelente fiabilidad interobservador (k = 0.940). Conclusión: Los resultados sugieren que la versión portuguesa produce resultados similares a la versión original, es sencilla de aplicar por la enfermera a la cabecera del paciente, fiable y con la sensibilidad adecuada para orientar a los profesionales sanitarios en la determinación de la necesidad de una valoración más completa.

Resumo Introdução: A disfagia é uma complicação frequente após o acidente vascular cerebral (AVC), estando associada a maior risco de pneumonia, desidratação e desnutrição. O rastreio precoce reduz a incidência de pneumonia após AVC. Objetivo: Traduzir, adaptar e testar a versão portuguesa do Gugging Swallowing Screen. Métodos: 226 doentes com AVC foram prospectivamente incluídos num estudo multicêntrico e o Gugging Swallowing Screen aplicado pelos enfermeiros no momento de admissão. Os resultados foram comparados com as pontuações da National Institute Health Stroke Scale, a Escala de Coma de Glasgow e o juízo clínico do enfermeiro. A fiabilidade entre avaliadores foi calculada. Resultados: A incidência de disfagia foi de 47.3%. A sensibilidade, a especificidade e a área sob a curva característica operatória do recetor foram, respetivamente, 85.4%, 84.6% e 0.892, usando o juízo clínico como referência. Foi estabelecida uma associação estatisticamente significativa entre rastreio positivo e maior déficit neurológico (χ2 = 32.99; p <0.001) e ausência de disfagia com coma leve (χ2 = 15.15; p <0.001). Foi alcançada uma excelente confiabilidade inter observadores (k = 0.940). Conclusão: Os resultados sugerem que a versão portuguesa produz resultados semelhantes à versão original, é de simples aplicação pelo enfermeiro à cabeceira do doente, confiável e com sensibilidade adequada para orientar os profissionais de saúde na determinação da necessidade de uma avaliação mais abrangente.

Abstract Introduction: Dysphagia is a frequent complication after a stroke which is associated with increased risk of pneumonia, dehydration and malnutrition. Early screening reduces the incidence of pneumonia after stroke. Objective: To translate, cross cultural adapt and test the Portuguese version of the Gugging Swallowing Screen. Methods: 226 acute stroke patients were prospectively enrolled in a multicentre study in stroke units. The Gugging Swallowing Screen was administered by nurses on patient’s admission. Results were compared to National Institute Health Stroke Scale and Glasgow Coma Scale scores and nurse’s clinical judgment. Inter-rater reliability was established. Results: The incidence of dysphagia using GUSS was 47.3%. Sensitivity, specificity and the area under the receiver operating characteristic curve were, respectively, 85.4%, 84.6% and 0.892., using nurse’s clinical judgment as a reference. A significant association was established between positive screening and a higher neurological deficit (χ2=32.99; p<0.001) and no dysphagia with mild coma (χ2=15.15; p<0.001). Excellent inter-rater reliability (k=0.940) was reached. Conclusion: The results suggest that the Portuguese version of the Gugging Swallowing Screen produces similar results as its original version, is simple to administer at bedside by nurses, is reliable and with adequate sensitivity to guide healthcare professionals in determining the need of a more comprehensive evaluation.

Resumo: Introdução: Desde o início da pandemia por COVID-19, desenvolveu-se a preocupação com o possível atraso no diagnóstico/tratamento dos doentes oncológicos. O nosso objetivo foi avaliar o impacto da pandemia no diagnóstico, tratamento e prognóstico dos doentes com cancro digestivo. Métodos: Estudo retrospetivo, incluindo doentes com diagnóstico inaugural de cancro digestivo, discutidos pela primeira vez na reunião semanal de oncologia digestiva (ROD) do nosso hospital, de Março-Agosto 2020 (grupo de estudo) e do período equivalente de 2018 (grupo controlo). Excluídos doentes com diagnóstico prévio de cancro digestivo/discussão prévia na ROD. Colheram-se: dados demográficos, origem da referenciação, estadio tumoral ao diagnóstico, data da primeira discussão na ROD, tratamento e prognóstico. Resultados: Incluídos 235 doentes, 107 no grupo de estudo (65.4% homens, idade média 71.59), 128 no grupo controlo (54.7% homens, idade média 68.16). Número médio de discussões clínicas semanais na ROD foi superior em 2018 (13.65 vs. 10.67, p = 0.040). Sem diferença estatisticamente significativa no número de doentes discutidos pela primeira vez na ROD (diagnóstico inaugural) entre os grupos (p = 0.670). Mais doentes referenciados à ROD do Serviço de Urgência (SU) em 2020, menos a partir do ambulatório/enfermaria (p < 0.001) e mais doentes referenciados após cirurgia urgente em 2020 (p = 0.022). Sem diferença entre os dois grupos no tempo médio de espera desde diagnóstico até a primeira discussão na ROD (p = 0.087). O estadio tumoral do cancro colorretal, gástrico e esofágico não foi significativamente diferente nos dois grupos (p = 0897, p = 0.168 e p = 0.717). Mais doentes apresentaram cancro pancreático em estadio IV no grupo de estudo (p = 0.043). Sem diferença no tempo desde ROD até início de quimioterapia neoadjuvante (p = 0.680) ou cirurgia eletiva (p = 0.198), nem da cirurgia até quimioterapia adjuvante (p = 0.396). Sem diferença na mortalidade aos 30 dias após primeira discussão na ROD nos dois grupos (p = 0.742). Conclusão: Durante a pandemia, o número de discussões clínicas na ROD foi inferior, mas o número de doentes com diagnóstico inaugural de cancro digestivo foi semelhante. No grupo de estudo, mais doentes foram referenciados à ROD do SU e mais após cirurgia urgente, sugerindo maior demora dos doentes para procurar atenção médica. Em 2020, os doentes não foram significativamente afetados pela pandemia relativamente à discussão atempada na ROD, início de tratamento ou mortalidade aos 30 dias, refletindo a manutenção da qualidade do suporte clínico aos doentes com cancro digestivo.

Abstract: Introduction: Since the rise of the COVID-19 pandemic there has been widespread concern regarding the possible delay in the diagnosis/treatment of cancer patients. We aimed to assess the impact of the COVID-19 pandemic on the diagnosis, treatment, and outcome of patients with digestive cancer. Methods: This was a retrospective study including patients with an inaugural digestive cancer diagnosis discussed for the first time at our center during the weekly digestive oncology reunion (DOR) meeting. The study group was enlisted from March to August 2020, and a control group was sourced from the equivalent period of 2018. Patients with a previous digestive cancer diagnosis/discussion in the DOR were excluded. The following data were collected: demographics, referral origin, tumor staging, first DOR discussion timing, treatment, and outcome. Results: A total of 235 patients were included: 107 in the study group (65.4% male, mean age 71.59 years); 128 in the control group (54.7% male, mean age 68.16 years). The mean number of clinical discussions per week was higher in 2018 (13.65 vs. 10.67, p = 0.040), without a difference in the mean number of patients discussed for the first time (inaugural diagnosis) between groups (p = 0.670). In the 2020 study group, more patients were referred to DOR from the emergency room (ER), fewer from the outpatient clinic/hospital wards (p < 0.001), and more were referred after urgent surgery (p = 0.022). There was no difference in the mean waiting time from diagnosis to first DOR discussion (p = 0.087). Tumor staging in colorectal, gastric, and esophageal cancer was not significantly different between the groups (p = 0897, p = 0.168, and p = 0.717). More patients in the study group presented with stage IV pancreatic cancer (p = 0.043). There was no difference in the time span from DOR until the beginning of neoadjuvant chemotherapy (p = 0.680) or elective surgery (p = 0.198), or from surgery until adjuvant chemotherapy (p = 0.396). Also, there was no difference in 30-day mortality from the first DOR date between the groups (p = 0.742). Conclusion: During the COVID-19 era there was a reduced number of clinical discussions in the DOR, but the number of debated patients with an inaugural digestive cancer diagnosis was similar. In the study group more patients were referred to DOR from the ER, and were referred after urgent surgery, suggesting a delayed demand for clinical attention. Study group patients were not significantly affected by the pandemic regarding timely DOR discussion, beginning of treatment, or 30-day mortality, reflecting the maintenance of the quality of care for digestive cancer patients.

BACKGROUND Calotropis procera latex protein fraction (LP) was previously shown to protect animals from septic shock. Further investigations showed that LP modulate nitric oxide and cytokines levels. OBJECTIVES To evaluate whether the protective effects of LP, against lethal bacterial infection, is observed in its subfractions (LPPII and LPPIII). METHODS Subfractions (5 and 10 mg/kg) were tested by i.p. administration, 24 h before challenging with lethal injection (i.p.) of Salmonella Typhimurium. LPPIII (5 mg/kg) which showed higher survival rate was assayed to evaluate bacterial clearance, histopathology, leukocyte recruitment, plasma coagulation time, cytokines and NO levels. FINDINGS LPPIII protected 70% of animals of death. The animals given LPPIII exhibited reduced bacterial load in blood and peritoneal fluid after 24 h compared to the control. LPPIII promoted macrophage infiltration in spleen and liver. LPPIII restored the coagulation time of infected animals, increased IL-10 and reduced NO in blood. MAIN CONCLUSIONS LPPIII recruited macrophages to the target organs of bacterial infection. This addressed inflammatory stimulus seems to reduce bacterial colonisation in spleen and liver, down regulate bacterial spread and contribute to avoid septic shock.

Abstract Purpose: To examine the effects of Arrabidaa chica (Bignoniacea) extract, a native plant of the Amazon known as crajiru, on a 7,12-dimethyl-1,2-benzanthracene (DMBA)-induced breast cancer model in Wistar rats. Methods: We compared the response of breast cancer to the oral administration of A. chica extract (ACE) for 16 weeks, associated or not with vincristine. Groups: normal control; DMBA (50mg/kg v.o,) without treatment; DMBA+ACE (300 mg/kg); DMBA+vincristine. 500μg/kg injected i.p; DMBA+ACE+Vincristine 250μg/kg i.p. Imaging by microPET and fluorescence, biochemistry, oxidative stress, hematology and histopathology were used to validate the treatments. Results: All animals survived. A gradual weight gain in all groups was observed, with no significant difference (p>0.05). The oral administration of ACE and ACE+vincristine 50% significantly reduced breast tumors incidence examined with PET-18FDG and fluorescence (p<0.001). Significant reduction of serum transaminases, oxidative stress and hematological toxicity were observed in these groups. Antioxidant enzyme levels in breast tissue were significantly higher compared to the DMBA and DMBA+vincristine groups. Conclusion: These results demonstrate for the first time that ACE positively influences the treatment of DMBA-induced breast cancer in animal model, inducing a reduction in oxidative stress and chemotherapy toxicity, meaning that ACE may have clinical implication in further studies.

Na suinocultura moderna, a busca de sistemas que promovam a qualidade e o bem-estar do meio ambiente é uma questão importante. Nesse sentido, o presente estudo avaliou os efeitos do enriquecimento ambiental sobre o desempenho e o comportamento de leitões. Em um delineamento em blocos ao acaso, 32 leitões (7,43kg de peso médio), desmamados aos 28 dias de idade, foram distribuídos em quatro tratamentos (controle sem enriquecimento, enriquecimento ambiental com cama de maravalha, enriquecimento com móbiles e cama + móbiles). Quatro repetições foram realizadas para cada tratamento, com dois animais por unidade experimental. A técnica de amostragem de varredura instantânea foi usada para registrar o comportamento de cada leitão, totalizando 20h. Os animais que receberam enriquecimento ambiental com cama + móbiles foram mais pesados aos 70 dias (P = 0,02), apresentaram maior ganho de peso total e diário (P = 0,04 e P = 0,02, respectivamente) e melhor conversão alimentar (P = 0,02). O enriquecimento ambiental aumentou a interação dos animais com o meio ambiente, o que lhes permite exibir comportamentos típicos.

In modern pig farming, the search for systems that promote environmental quality and welfare is an important issue. In this sense, the present study evaluated the effects of environmental enrichment on the performance and behavior of piglets. In a completely randomized block design, 32 piglets (7.43kg mean weight), weaned at 28 days of age, were distributed into four treatments (control without enrichment, environmental enrichment with wood shavings as bedding; environmental enrichment with hanging toys and environmental enrichment with wood shavings + hanging toys). Four repetitions were performed for each treatment, with two animals per experimental unit. The instant scan sampling technique was used to record the behavior of each piglet for 20h. Animals receiving environmental enrichment with wood shavings + hanging toys were heavier at 70 days (P=0.02), exhibited higher total and daily weight gain (P=0.04 and P=0.02, respectively) and better feed conversion (P=0.02). Environmental enrichment increased the interaction of animals with the environment, allowing them to exhibit natural behaviors.

Resumo:O colapso induzido pelo exercício (EIC) é considerado uma síndrome autossômica recessiva que afeta principalmente cães da raça Labrador Retriever. A doença é caracterizada por fraqueza muscular e colapso após exercício intenso. Usualmente, ocorre recuperação clínica após o episódio, mas alguns animais podem vir a óbito. Os sinais clínicos são decorrentes do polimorfismo de base única (SNP) c.767G>T no gene Dynamin 1 (DNM1). O objetivo deste trabalho foi determinar a ocorrência deste SNP em 321 cães da raça Labrador Retriever do Estado de São Paulo. Primers específicos para a amplificação de todo o exon 6 do gene DNM1 foram usados nas PCRs utilizando DNA a partir de amostras de sangue ou swab bucal, a avaliação final foi realizada com sequenciamento direto dos produtos da PCR. Dentre os 321 animais estudados, 3,4 % (11/321) eram homozigotos para o SNP c.767G>T no gene DNM1 e 24,6% (79/321) eram heterozigotos. Somente um dos 11 animais homozigotos apresentavam sinais clínicos compatíveis com a EIC. Este é o primeiro estudo sobre a ocorrência deste SNP no Brasil e considerando que quase 25% dos animais estudados eram heterozigotos, a genotipagem dos animais para este SNP pode ser importante antes dos acasalamentos para cães desta raça. A EIC deve ser considerada nos diagnósticos diferenciais de enfermidades neuromusculares em cães da raça Labrador Retriever.

Abstract:The exercise-induced collapse (EIC) is considered an autosomal recessive syndrome that mainly affects Labrador Retriever dogs. The disease is characterized by muscle weakness and collapse after intense exercise. Recovery usually occurs after exercise but some animals may die. The clinical signs occurs due to the single-nucleotide polymorphism (SNP) c.767G>T in Dynamin 1 (DNM1) gene. The aim of this study was to evaluate the occurrence of this SNP in 321 Labrador Retriever dogs from São Paulo state. Specific primers for amplification of the entire exon 6 of the DNM1 gene were used in a PCR performed with DNA from blood or buccal swab samples, direct sequencing was performed for the final evaluation. Among 321 animals studied, 3.4% (11/321) of animals were homozygous for the DNM1 SNP (c.767G>T) and 24.6% (79/321) were heterozygous. Only one of the 11 homozygous animals in this study had previous clinical signs compatible with this disease. This is the first study that evaluated the occurrence of DNM1 SNP (c.767G>T) gene in Brazil and considering that almost 25% of the studied animals were heterozygous, the routinely evaluation of this SNP may be important before this breed mating The EIC should be include in the differential diagnosis of neuromuscular diseases in Labrador Retriever dogs.

The zoonotic potential to cause human and/or animal infections among multidrug-resistant extraintestinal pathogenic Escherichia coli from avian origin was investigated. Twenty-seven extraintestinal pathogenic E. coli isolates containing the increased survival gene (iss) were obtained from the livers of healthy and diseased poultry carcasses at two slaughterhouses in Salvador, northeastern Brazil. The antimicrobial resistance-susceptibility profiles were conducted with antibiotics of avian and/or human use by the standardized disc-diffusion method. Antimicrobial resistance was higher for levofloxacin (51.8%), amoxicillin/clavulanic acid (70.4%), ampicillin (81.5%), cefalotin (88.8%), tetracycline (100%) and streptomycin (100%). The minimum inhibitory concentrations above the resistance breakpoints of doxycycline, neomycin, oxytetracycline and enrofloxacin reached, respectively, 88.0%, 100%, 75% and 91.7% of the isolates. Strains with high and low antimicrobial resistance were i.p. administered to Swiss mice, and histopathological examination was carried out seven days after infection. Resistance to goat and human serum complement was also evaluated. The results show that Swiss mice challenged with strain 2B (resistant to 11 antimicrobials) provoked a severe degeneration of hepatocytes besides lymphocytic infiltration in the liver, whereas the spleen showed areas of degeneration of the white and red pulp. Conversely, the spleen and liver of mice challenged with strain 4A (resistant to two antimicrobials) were morphologically preserved. In addition, complement resistance to goat and human serum was high for strain 2B and low for strain 4A. Our data show that multidrug resistance and pathogenesis can be correlated in extraintestinal pathogenic E. coli strains obtained from apparently healthy poultry carcasses, increasing the risk for human public healthy.

Um estudo virológico e sorológico seccional (E1) e outro longitudinal (E2) foram realizados em granjas com (G2 e G3) e sem (G1) a síndrome de refugagem multissitêmica (SRM) no Brasil. Foram coletadas amostras de sangue, soro, swabs nasal e retal de animais de cada categoria do ciclo produtivo: porcas, leitões maternidade, creche, recria e terminação. Em E1, nas granjas G1a e G2, foram amostrados 40 animais de cada categoria. Em E2, nas granjas G1b e G3, 35 leitões na maternidade foram identificados e amostrados ao longo do ciclo produtivo. O soro foi avaliado para presença de anticorpos contra circovírus suíno tipo 2 (CVS2) e sangue e swabs para presença do ácido nucléico viral. Em E1, a categoria porcas possuía altas taxas de animais virêmicos e soropositivos, com porcentagem de porcas com títulos altos superior a G2. Em G1a a queda de imunidade passiva ocorreu entre o final da fase de creche e início da recria com aumento da eliminação viral em swabs e subsequente soroconversão. Em G2 a queda ocorreu entre a fase final da maternidade e início da creche, com diminuição da eliminação viral. Em E2, a queda da imunidade materna ocorreu entre a 1ª e 2ª coleta em G1b; e em G3, entre a 2ª e 3ª coleta. Em ambas as granjas, a queda de imunidade passiva coincidiu com o aumento da viremia e eliminação viral e a soroconversão ocorreu entre a 3ª e 4ª coleta em ambas as granjas com aumento da média de título de anticorpos e declínio da viremia. Viremia e eliminação viral foram detectadas em todas as coletas realizadas; 42% dos animais amostrados em E2 foram virêmicos em todas as coletas e todas as amostras de tecido coletadas no abate foram positivas para o CVS2. Este estudo confirma a persistência da viremia mesmo em presença de altos títulos de anticorpos e que o perfil sorológico em um rebanho com e sem a presença da síndrome pode ser diferente, principalmente em relação à duração da imunidade passiva.

A virological and serological cross-sectional study (E1) and a longitudinal study (E2) were performed on herds with (G2 and G3) and without (G1) post weaning multisystemic syndrome (PMWS) in Brazil. Blood, serum, nasal and rectal swabs samples were collected of sows, farrowing piglets, nursery, growing and finishing pigs. In E1, were sampled 40 animals in each category (G1a and G2). In E2, (G1b and G3), 35 farrowing piglets were identified and sampled along the production cycle. Porcine circovirus type 2 (PCV2) antibodies were assayed. A PCR was used to detected PCV2 genome in blood and swabs. In E1, sows had high rates of viremic and seropositives animals, with percentage of sows with high antibodies titers greater than G2. Passive antibodies decline occurred between nursery and growing area with increased viral shedding in swabs and subsequent seroconversion in G1. In G2, the passive antibodies decay occurred in nursery, with a reduction in viral shedding. In E2, the decline of maternal immunity occurred between the 1st and 2nd collection in G1b, and between 2nd and 3rd collections in G3. In both herds, the decay of passive immunity coincided with increased viremia and viral shedding; and seroconversion occurred between the 3rd and 4th collection in both herds with decline of viremia. Viremia and viral shedding was detected in all samples days, 42% of animals sampled in E2 were viremic and all tissue samples collected at slaughter were positive for PCV2. This study confirms the persistence of viremia even in the presence of high titers of antibodies and the serological profile in a herd with or without PMWS may be different, especially with regard to the passive immunity duration.