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A new species of armored catfish of the genus Scobinancistrus (Loricariidae: Hypostominae) from the Xingu River basin, Brazil
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Chaves, Mateus S.

; Oliveira, Renildo R.

; Gonçalves, Alany P.

; Sousa, Leandro M.

; Py-Daniel, Lúcia H. Rapp

Neotropical Ichthyology

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2023, Volume 21 N. 3 elocation e230038

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract A new species of Scobinancistrus from the Xingu River, Brazil, is described. It can be distinguished from its congeners by color pattern and a combination of non-exclusive characters: overall body covered by large yellow spaced blotches over a dark background (vs. small round and densely packed spots over light or dark background in S. pariolispos and S. aureatus); lack of orange to yellow/orange distal band on dorsal and caudal fins (vs. presence in S. aureatus), dorsal fin not reaching adipose-fin supporting plate when adpressed (vs. reaching the adipose-fin plate in S. pariolispos and S. aureatus). The new species is only known from a portion of the middle Xingu River, ranging from the Volta Grande do Xingu, an area under a strong anthropic impact due to the construction of the Belo Monte dam, to near the Iriri River confluence with the Xingu River. Aspects concerning the species’ threats and its conservation status are discussed.

Resumo Uma nova espécie de Scobinancistrus do rio Xingu, Brasil, é descrita. Ela pode ser distinguida de suas congêneres pelo padrão de cores e uma combinação de caracteres não exclusivos: corpo geral coberto por grandes manchas amarelas espaçadas sobre um fundo escuro (vs. pequenos pontos redondos e densamente compactados sobre fundo claro ou escuro em S. pariolispos e S. aureatus); falta de banda distal laranja a amarela nas nadadeiras dorsal e caudal (vs. presença em S. aureatus), nadadeira dorsal não alcançando a placa da nadadeira adiposa quando comprimida (vs. atingindo a placa da nadadeira adiposa em S. pariolispos e S. aureatus). A nova espécie só é conhecida de uma porção do médio rio Xingu, que vai da Volta Grande do Xingu, área de forte impacto antrópico devido à construção da barragem de Belo Monte, até próximo à confluência do rio Iriri com o rio Xingu. Aspectos relativos às ameaças à espécie e seu estado de conservação são discutidos.

https://doi.org/10.1590/1982-0224-2023-0038

Evaluation of temporary cement to prevent bacterial contamination of the root canal after pulpectomy
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PEREIRA, Letícia Martins

; NAVES, Sophia Maria Mateus

; COSTA, Júlia Modesto da

; MORAES, Igor de Oliveira

; SILVA, Sandra Regina Echeverria Pinho da

; FONTANA, Carlos Eduardo

; PINHEIRO, Sérgio Luiz

RGO - Revista Gaúcha de Odontologia

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2022, Volume 70 elocation e20220035

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Avaliar a capacidade do obturador provisório (OP) e da gutapercha em bastão (GP) de prevenir a contaminação bacteriana dos condutos radiculares. Foram selecionados 80 incisivos centrais superiores decíduos artificiais que foram divididos aleatoriamente em 2 grupos: OP (n = 40) e GP (n = 40). Foi realizado acesso endodôntico, instrumentação rotatória, preenchimento do canal radicular e selamento coronário conforme os grupos. Foi feita a semeadura de S.mutans e E. faecalis na entrada do canal radicular. Ambos os grupos foram subdivididos em 5 tempos experimentais (24, 48, 72, 96 e 120 horas), com 8 espécimes por tempo experimental: 5 submetidos a preenchimento do canal radicular e selamento coronário (com OP ou GP) e 3 controles (apenas selamento coronário, sem preenchimento do canal). Todos os espécimes foram incubados em jarras de anaerobiose a 37°C e a contaminação bacteriana foi avaliada em espectrofotômetro. Utilizou-se ANOVA (teste t) para a comparação da contaminação e o teste de Kruskal-Wallis para a comparação dos escores da obturação entre os grupos experimentais. Foi observada diferença significativa no selamento nas primeiras 24 horas entre GP e controles (p = 0,046). Não houve diferença estatisticamente significativa no padrão de preenchimento entre os canais selados com OP versus GP. Os espécimes selados com GP apresentaram menor contaminação do que os controles nas primeiras 24 horas. Nos demais tempos experimentais, tanto GP quanto OP não foram eficientes no controle da contaminação bacteriana; ambos apresentaram falha no selamento coronário.

ABSTRACT To evaluate the ability of temporary cement (TC) and gutta-percha sticks (GP) to prevent bacterial contamination of the root canal through the coronal seal after pulpectomy. Eighty artificial primary maxillary central incisors were selected and randomly divided into 2 groups: TC (n = 40) and GP (n = 40). Endodontic access, rotary instrumentation, root canal filling, and coronal sealing were performed according to group allocation. The root canal opening was seeded with S. mutans and E. faecalis. Both groups were subdivided into 5 experimental time points (24, 48, 72, 96, and 120 hours), with 8 specimens per time-point group: 5 in which both root canal filling and coronal sealing were performed (with either TC or GP) and 3 controls (coronal sealing alone, without root canal filling). All specimens were incubated in an anaerobic jar at 37°C, and bacterial contamination was assessed in a spectrophotometer. ANOVA (t-test) was used to compare contamination and the Kruskal-Wallis test to compare filling scores between the experimental groups. A significant difference was observed in sealing in the first 24 hours between GP and controls (p = 0.046). There was no significant difference in the filling pattern between canals sealed with TC versus GP. Specimens sealed with GP showed less contamination than controls in the first 24 hours. At later time points, neither GP nor TC were effective at controlling bacterial contamination; both failed to provide adequate coronal sealing.

https://doi.org/10.1590/1981-86372022003520210014

Lipid-matrix effects on tyrosinase immobilization in Langmuir and Langmuir-Blodgett films
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PEREIRA, MATHEUS S.

; MAXIMINO, MATEUS D.

; MARTIN, CIBELY S.

; AOKI, PEDRO H.B.

; OLIVEIRA JR, OSVALDO N.

; ALESSIO, PRISCILA

Anais da Academia Brasileira de Ciências

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2021, Volume 93 N. 1 elocation e20200019

Abstract: En | Text: En | PDF: En

Abstract The immobilization of the enzyme tyrosinase (Tyr) in lipid matrices can be explored to produce biosensors for detecting polyphenols, which is relevant for the food industry. Herein, we shall demonstrate the importance of the lipid composition to immobilize the enzyme tyrosinase in Langmuir-Blodgett (LB) films. Tyr could be incorporated into Langmuir monolayers of arachidic acid (AA), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phospho-(1’-rac-glycerol) (sodium salt) (DPPG), having as the main effect an expansion in the monolayers. Results from polarization-modulated infrared reflection-absorption spectroscopy (PM-IRRAS) pointed to electrostatic interactions between the charged residues of Try and the lipid headgroups, in addition to changes in the order of lipid chains. The interaction between Tyr and DPPC in Langmuir monolayers can be correlated with the superior performance of DPPC/Tyr LB films used as biosensors to detect catechol by cyclic voltammetry. The molecular-level interactions assessed via PM-IRRAS are therefore believed to drive an immobilization process for Tyr in the lipid LB matrix and may serve as a general criterion to identify matrices that preserve enzyme activity.

https://doi.org/10.1590/0001-3765202120200019 402 downloads

Bloqueio de Receptores AT1 Melhora o Desempenho Funcional Miocárdico na Obesidade
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Oliveira-Junior, Silvio Assis de

; Muzili, Nayara de Araújo

; Carvalho, Marianna Rabelo de

; Ota, Gabriel Elias

; Morais, Camila Souza de

; Vieira, Larissa Fregapani da Costa

; Ortiz, Mateus Oliveira

; Campos, Dijon H. S.

; Cezar, Marcelo Diacardia Mariano

; Okoshi, Marina Politi

; Okoshi, Katashi

; Cicogna, Antonio C.

; Martinez, Paula Felippe

Arquivos Brasileiros de Cardiologia

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jul 2020, Volume 115 N. 1 Pages 17 - 28

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Fundamento A obesidade tem sido associada com ativação crônica do sistema renina-angiotensina-aldosterona e importantes alterações no desempenho cardíaco. Objetivo Avaliar a influência do bloqueio de receptores de angiotensina-II do tipo 1 (AT1) sobre a morfologia e desempenho cardíaco de ratos obesos por dieta Métodos Ratos Wistar (n=48) foram submetidos a dieta controle (2,9 kcal/g) ou hiperlipídica (3,6 kcal/g) durante 20 semanas. Após a 16ª semana, foram distribuídos em quatro grupos: Controle (CO), Obeso (OB), Controle Losartan (CL) e Obeso Losartan (OL). CL e OL receberam losartan (30 mg/kg/dia) na água durante quatro semanas. Posteriormente, foram analisadas composição corporal, pressão arterial sistólica (PAS) e ecocardiograma. A função de músculos papilares foi avaliada em situação basal com concentração de cálcio ([Ca2+]o) de 2,50 mM e após manobras inotrópicas: potencial pós-pausa (PPP), elevação da [Ca2+]o e durante estimulação beta-adrenérgica com isoproterenol. A análise dos resultados foi feita por meio de Two-Way ANOVA e teste de comparações apropriado. O nível de significância considerado foi de 5%. Resultados Embora a alteração da PAS não tenha se mantido ao final do experimento, a obesidade se associou com hipertrofia cardíaca e maior velocidade de encurtamento da parede posterior do ventrículo esquerdo.No estudo de músculos papilares em condição basal, CL mostrou menor velocidade máxima de variação negativa da tensão desenvolvida (-dT/dt) do que CO. O PPP de 60s promoveu menor -dT/dt e pico de tensão desenvolvida (TD) em OB e CL, comparados ao CO, e maior variação relativa de TD e velocidade máxima de variação positiva (+dT/dt) no OL em relação a CL e OB. Sob 1,5, 2,0 e 2,5mM de [Ca2+]o, o grupo OL exibiu maior -dT/dt do que CL. Conclusão Losartan melhora a função miocárdica de ratos com obesidade induzida por dieta. (Arq Bras Cardiol. 2020; 115(1):17-28)

Abstract Background Obesity has been associated with chronic activation of the renin-angiotensin-aldosterone system and with significant changes in cardiac performance. Objective To assess the impact of a blockade of angiotensin-II receptor type 1 (AT1receptor) on morphology and on myocardial functional performance in rats with high-fat diet- induced obesity. Methods Wistar rats (n=48) were submitted to control (2.9 kcal/g) or high-fat (3.6 kcal/g) diet for 20 weeks. After the 16thweek they were divided into four groups: Control (CO), Obese (OB), Control Losartan (CL) and Obese Losartan (OL). CL and OL received losartan (30 mg/kg/day) in drinking water for four weeks. Subsequently, body composition, systolic blood pressure (SBP) and echocardiographic variables were analyzed. Papillary muscle function was assessed at baseline with 2.50 mM calcium concentration ([Ca2+]o) and after inotropic maneuvers: post-pause potentiation (PPP), [Ca2+]oelevation, and during beta-adrenergic stimulation with isoproterenol. Analysis of the results was performed by the Two-Way ANOVA and by the appropriate comparison test. The level of significance was set at 5%. Results Although SBP change had been not maintained at the end of the experiment, obesity was associated with cardiac hypertrophy and with increased left ventricle posterior wall shortening velocity. In the study of papillary muscles in basal condition, CL showed lower developed tension maximum negative variation velocity (-dT/dt) than CO. The 60s PPP promoted lower -dT/dt and maximum developed tension (DT) in OB and CL compared with CO, and higher relative DT variation and maximum positive variation velocity (+dT/dt) in OL compared with CL and OB. Under 1.5, 2.0, and 2.5mM [Ca2+]o, the OL group showed higher -dT/dt than CL. Conclusion Losartan improves myocardial function in high-fat diet-induced obesity. (Arq Bras Cardiol. 2020;115(1):17-28)

https://doi.org/10.36660/abc.20190131 680 downloads

COVID-19 e Eventos Coronários Agudos – Danos Colaterais. Um Relato de Caso
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Ritt, Luiz Eduardo Fonteles

; Viana, Mateus S.

; Feitosa, Gustavo Freitas

; Oliveira, Adriano Martins de

; Souza, Fabio Solano

; Darzé, Eduardo Sahade

Arquivos Brasileiros de Cardiologia

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jun 2020, Volume 114 N. 6 Pages 1072 - 1075

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.36660/abc.20200329 1944 downloads

VALIDAÇÃO DE MÉTODO ESPECTROFOTOMÉTRICO DE ANÁLISE PARA A QUANTIFICAÇÃO DE ÁCIDO ACETILSALICÍLICO EM FORMULAÇÕES FARMACÊUTICAS: UMA PROPOSTA DE AULA EXPERIMENTAL PARA ANÁLISE INSTRUMENTAL
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Goes Junior, Enock José A.

; Roeder, Jakson S.

; Oliveira, Kaiky B. L.

; Ferreira, Mateus P.

; Silva, Jonatas G. da

Química Nova

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jan 2019, Volume 42 N. 1 Pages 99 - 104

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

In the present article it is described the validation of a simple, precise, accurate, rapid and low-cost UV spectrophotometric method for the quantification of acetylsalicylic acid in pharmaceutical formulations. The method was validated by linearity, specificity, range, accuracy, precision, robustness, and limits of detection and quantification. The method was linear in the range between 24 – 36 µg mL-1, presenting a good correlation coefficient (r = 0.9994) and adequate limits of quantification (6.57 µg mL-1) and detection (1.99 µg mL-1). Precision analysis showed low coefficient of variation (< 2.0%) and a good average recovery percentual (99.42% - reference and 95.94% - generic). The results and the statistical treated proved that the method may be considered a precise, accurate and low cost analytical alternative for laboratory routine and experimental activity. The method was applied for acetylsalicylic acid determination in two different pharmaceuticals formulations. The results of these analyzes are in accordance with the values declared by the manufacturer, at a 95% confidence level. This experimental activity is a simple and might be used as an practice activities on teaching laboratories improve the student learning.

https://doi.org/10.21577/0100-4042.20170300 2544 downloads

Phytochemical screening and antimicrobial activity testing of crude hydroalcoholic extract from leaves of Sphagneticola trilobata (Asteraceae)
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Leite, Ana Greice Borba

; Farias, Eulina Tereza Nery

; Oliveira, Ana Paula de

; Abreu, Ruan Emmanuell Franco de

; Costa, Mateus Matiuzzi da

; Almeida, Jackson Roberto Guedes da Silva

; Estevão, Lígia Reis de Moura

; Evêncio-Neto, Joaquim

Ciência Rural

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2019, Volume 49 N. 4 elocation e20180639

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: Este estudo teve como objetivo realizar a triagem fitoquímica preliminar e testar a atividade antimicrobiana do extrato hidroalcoólico bruto das folhas de Sphagneticola trilobata. A identificação das classes de metabólitos secundários presentes no extrato foi realizada através da cromatografia em camada delgada analítica (CCDA). Para determinar a quantidade de fenóis e flavonoides totais foram utilizados os métodos espectrofotométricos de Folin-Ciocalteu e complexação com AlCl3, respectivamente. Para avaliar a atividade antimicrobiana foram testadas culturas de Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus spp., Escherichia coli, Serratia marcescens, Enterococcus faecalis, Pseudomonas aeruginosa, Salmonella Typhimurium, Klebsiella pneumoniae isoladas de pele humana e culturas de Staphylococcus spp. isoladas de pele de cães pelo método de microdiluição em caldo. Na triagem fitoquímica foi verificada reação positiva para a presença de derivados antracênicos, mono, sesqui e diterpenos. As análises colorimétricas mostraram conteúdos de fenóis totais e flavonoides totais de 21,7 ± 0,009 miligramas de equivalentes de ácido gálico por grama de amostra e 0,23 ± 0,005 miligramas de equivalentes de catequina por grama de amostra, respectivamente. Na análise microbiológica, o extrato hidroalcoólico das folhas de Sphagneticola trilobata apresentou atividade antimicrobiana frente às culturas de Staphylococcus spp., Escherichia coli, Serratia marcescens e Enterococcus faecalis. Todas as culturas de Staphylococcus spp. isoladas de pele de cães foram sensíveis ao extrato. Conclui-se que o extrato hidroalcoólico bruto das folhas de Sphagneticola trilobata possui entre seus metabólitos secundários os flavonoides e terpenoides que contribuíram com a atividade antimicrobiana frente às bactérias isoladas de pele de diferentes origens.

ABSTRACT: This study aimed to perform phytochemical analysis and to test the antimicrobial activity of the crude hydroalcoholic extract obtained from the leaves of Sphagneticola trilobata. Classes of secondary metabolites present in the extract were identified through phytochemical screening using analytical thin-layer chromatography. Antimicrobial activity was evaluated by testing cultures of Staphylococcus aureus, S. epidermidis, Staphylococcus spp., Escherichia coli, Serratia marcescens, Enterococcus faecalis, Pseudomonas aeruginosa, Salmonella Typhimurium, and Klebsiella pneumoniae isolated from human skin and those of Staphylococcus spp. isolated from dog skin using the broth microdilution method. In the phytochemical screening, classes of anthracenic derivatives and mono-, sesqui-, and diterpenes were identified. Colorimetric analysis showed total phenol and total flavonoid contents of 21.7 ± 0.009 mg of gallic acid equivalents per gram of sample and 0.23 ± 0.005 mg of catechin equivalents per gram of sample, respectively. Microbiological analysis revealed that the hydroalcoholic extract of S. trilobata exhibited antimicrobial activity against cultures of Staphylococcus spp., E. coli, S. marcescens, and E. faecalis isolated from human skin and those of Staphylococcus spp. isolated from dog skin. Thus, crude hydroalcoholic extract of leaves of S. trilobata contained flavonoids and terpenoids as secondary metabolites, which contributed to its antimicrobial activity against skin bacteria isolated from different sources.

https://doi.org/10.1590/0103-8478cr20180639 1514 downloads

Combination of fixative agents and fixation times to visually differentiate the cortical from the medullary layer in bovine adrenal glands
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OLIVEIRA, FABRÍCIO S.

; MACITELLI, FERNANDA

; SANTOS, CAIO C.C.

; BRAGA, JANAINA S.

; COSTA, BARBARA C.

; PASTORE, MARINA

; ROCHA, THIAGO A.S.S.

; COSTA, MATEUS J.R.P. DA

Anais da Academia Brasileira de Ciências

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dec 2018, Volume 90 N. 4 Pages 3887 - 3891

Abstract: En | Text: En | PDF: En

Abstract Chronic stress exposure commonly increases adrenals weight and changes their morphology. This study aimed to compare four methods to delimitate the cortical and medullary layers of adrenals glands in Nelore bulls. Fresh adrenals did not present differentiation between layers. Then, frozen adrenals were distributed in plastics bags with fixative Bouin (G1), 96ºGL ethylic alcohol (G2), 10% formaldehyde (G3), or 2.5% glutaraldehyde (G4). After 12 hours of fixation, the G1 adrenal glands did not show the entire cortical layer marked by Bouin’s solution. For G2 and G3 there was a poor contrast, while for G4 there was a reasonable contrast. After 24 hours of fixation, G1 had an excellent contrast between layers, while G2 and G4 had a reasonable contrast and G3 a very bad contrast. After 48 hours it was difficult to differentiate cortical and medullar layers for G1; for Group 2 we get a reasonable contrast; and for G3 the contrast was bad. For G4 the contrast was not as sharp due to the medulla became dark. It was concluded that fixation of adrenals must be done in Bouin’s solution for 24 hours to obtain an effective evaluation of the adrenals’ morphometry.

https://doi.org/10.1590/0001-3765201820170941 670 downloads

Frequency of Staphylococcus aureus virulence genes in milk of cows and goats with mastitis
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Acosta, Atzel C.

; Oliveira, Pollyanne Raysa F.

; Albuquerque, Laís

; Silva, Isamara F.

; Medeiros, Elizabeth S.

; Costa, Mateus M.

; Pinheiro Junior, José Wilton

; Mota, Rinaldo A.

Pesquisa Veterinária Brasileira

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nov 2018, Volume 38 N. 11 Pages 2029 - 2036

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O presente estudo determinou a frequência de genes de virulência de Staphylococcus aureus em 2253 amostras de leite, sendo de vacas n=1000 e de cabras n=1253, procedentes das três regiões geográficas do estado de Pernambuco, Brasil. A presença de genes de fatores de virulência associados à adesão às células hospedeiras (fnbA, fnbB, clfA e clfB), toxinosis (sea, seb, sec, sed, seg, seh, sei, tsst, hla e hlb) e polissacarídeo capsular (cap5 e cap8) foram avaliadas por PCR. Um total de 123 e 27 cepas de S. aureus foram isoladas do leite de vacas e cabras, respectivamente. Os genes sec e tsst foram detectados exclusivamente em isolados de cabras, enquanto o gene seh foi identificado apenas em isolados de vaca. O número de genes de toxina por cepa mostrou que os isolados de cabras são potencialmente mais tóxicos do que os isolados obtidos de bovinos. O genótipo cap5 predominou em ambas as espécies hospedeiras, especialmente em cepas coletadas de vacas criadas na região Agreste. O genótipo cap8 é potencialmente mais virulento devido ao número de genes de virulência por isolado. Os resultados do presente estudo demonstram que S. aureus pode representar uma ameaça potencial para a saúde humana no Brasil e, portanto, estes resultados devem subsidiar ações relacionadas aos programas de controle de mastite.

ABSTRACT: The present study determined the frequency of Staphylococcus aureus virulence genes in 2,253 milk samples of cows (n=1000) and goats (n=1253) raised in three different geographical regions of the state Pernambuco, Brazil. The presence of genes of virulence factors associated to adhesion to host cells (fnbA, fnbB, clfA and clfB), toxinosis (sea, seb, sec, sed, seg, seh, sei, tsst, hla and hlb), and capsular polysaccharide (cap5 and cap8) was evaluated by PCR. A total of 123 and 27 S. aureus strains were isolated from cows’ and goats’ milk, respectively. The sec and tsst genes were detected exclusively in goats’ isolates, while the seh gene was only identified in cows’ isolates. The number of toxin genes per strain showed that goats’ isolates are likely more toxic than bovines’ isolates. The cap5 genotype predominated in both host species, especially in strains collected from cows raised in the Agreste region. The cap8 genotype is likely more virulent due to the number of virulence genes per strain. The results of the present study demonstrate that S. aureus may pose a potential threat to human health in Brazil, and, therefore, these results should support actions related to mastitis control programs.

https://doi.org/10.1590/1678-5150-pvb-5786 1600 downloads

10.

Tailored Silica Nanoparticles Surface to Increase Drug Load and Enhance Bactericidal Response
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Oliveira, Luciane F. de

; Bouchmella, Karim

; Picco, Agustin S.

; Capeletti, Larissa B.

; Gonçalves, Kaliandra A.

; Santos, João Henrique Z. dos

; Kobarg, Jörg

; Cardoso, Mateus B.

Journal of the Brazilian Chemical Society

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sep 2017, Volume 28 N. 9 Pages 1715 - 1724

Abstract: En | Text: En | PDF: En

Nanoparticles' surface properties can be used as triggers to regulate or even enhance biological response and generate tailored structures to substitute conventional antibiotics. Here, silica nanoparticles surface was duly tuned in order to increase the water-insoluble drug load (curcumin) and improve the antibacterial activity. Our main motivation was based on the electrostatic attraction between the positively charged amino groups and the negatively charged curcumin and/or bacteria membrane. In addition, the variation of amino grafting amount on silica nanoparticles indicated that the grafting increase was directly related to the extent of drug entrapped into the nanoparticles as well as to the bactericidal activity. The combination of amino-functionalized silica nanoparticles associated with the presence of curcumin allowed to produce a dual bactericidal system that shows promising perspective for its use in biomedical applications.

https://doi.org/10.21577/0103-5053.20170017 8027 downloads

11.

Variações e diferenciais da mortalidade por doença cardiovascular no Brasil e em seus estados, em 1990 e 2015: estimativas do Estudo Carga Global de Doença
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Brant, Luisa Campos Caldeira

; Nascimento, Bruno Ramos

; Passos, Valéria Maria Azeredo

; Duncan, Bruce Bartholow

; Bensenõr, Isabela Judith Martins

; Malta, Deborah Carvalho

; Souza, Maria de Fatima Marinho de

; Ishitani, Lenice Harumi

; França, Elizabeth

; Oliveira, Mateus S.

; Mooney, Meghan

; Naghavi, Mohsen

; Roth, Gregory

; Ribeiro, Antonio Luiz Pinho

Revista Brasileira de Epidemiologia

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may 2017, Volume 20 Suppl. 1 Pages 116 - 128

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: Objetivo: Analisar as variações e os diferenciais da mortalidade por doenças cardiovasculares (DCV) no Brasil e em seus estados, em 1990 e 2015. Métodos: Foram utilizados os dados de mortalidade compilados pelo Global Burden of Disease (GBD) 2015, obtidos da base de dados do Sistema de Informação sobre Mortalidade do Ministério da Saúde. Foram realizadas a correção do sub-registro de óbitos e a reclassificação dos códigos garbage por meio de algoritmos específicos. As causas cardiovasculares foram subdivididas em 10 causas específicas. As taxas de mortalidade - dos anos 1990 e 2015 - foram padronizadas pela idade, de acordo com o sexo e o estado brasileiro. Resultados: A taxa de mortalidade por DCV padronizada por idade caiu de 429,5 (1990) para 256,0 (2015) a cada 100 mil habitantes (40,4%). A redução proporcional foi semelhante em ambos os sexos, mas as taxas em homens são substancialmente mais altas do que nas mulheres. A redução da taxa padronizada por idade foi mais acentuada para a doença cardíaca reumática (44,5%), cardiopatia isquêmica (43,9%) e doença cerebrovascular (46,0%). A queda na mortalidade diferiu marcadamente entre os estados, sendo mais acentuada nos estados das regiões Sudeste e Sul do país e no Distrito Federal, e atenuada nos estados do Norte e Nordeste. Conclusão: A mortalidade por DCV padronizada por idade reduziu no Brasil nas últimas décadas, porém de forma heterogênea entre os estados e para diferentes causas específicas. Considerando a magnitude da carga de doença e o envelhecimento da população brasileira, as políticas de enfrentamento das DCV devem ser priorizadas.

ABSTRACT: Objective: To analyze variations and particularities in mortality due to cardiovascular disease (CVD) in Brazil and in Brazilian states, in 1990 and 2015. Methods: We used data compiled from the Global Burden of Disease (GBD) 2015, obtained from the database of the Mortality Information System (SIM) of the Brazilian Ministry of Health. Correction of the sub-registry of deaths and reclassification of the garbage codes were performed using specific algorithms. The cardiovascular causes were subdivided into 10 specific causes. Age-standardized CVD mortality rates - in 1990 and 2015 - were analyzed according to sex and Brazilian state. Results: Age-standardized CVD mortality rate decreased from 429.5 (1990) to 256.0 (2015) per 100,000 inhabitants (40.4%). The proportional decrease was similar in both sexes, but death rates in males were substantially higher. The reduction of age-standardized mortality rate was more significant for rheumatic heart disease (44.5%), ischemic cardiopathy (43.9%), and cerebrovascular disease (46.0%). The decline in mortality was markedly different across states, being more pronounced in those of the southeastern and southern regions and the Federal District, and more modest in most states in the north and northeast regions. Conclusion: Age-standardized CVD mortality has declined in Brazil in recent decades, but in a heterogeneous way across states and for different specific causes. Considering the burden magnitude and the Brazilian population aging, policies to prevent and manage CVD should continue to be prioritized.

https://doi.org/10.1590/1980-5497201700050010 1557 downloads

12.

Variações e diferenciais da mortalidade por doença cardiovascular no Brasil e em seus estados, em 1990 e 2015: estimativas do Estudo Carga Global de Doença
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Brant, Luisa Campos Caldeira

; Nascimento, Bruno Ramos

; Passos, Valéria Maria Azeredo

; Duncan, Bruce Bartholow

; Bensenõr, Isabela Judith Martins

; Malta, Deborah Carvalho

; Souza, Maria de Fatima Marinho de

; Ishitani, Lenice Harumi

; França, Elizabeth

; Oliveira, Mateus S.

; Mooney, Meghan

; Naghavi, Mohsen

; Roth, Gregory

; Ribeiro, Antonio Luiz Pinho

Revista Brasileira de Epidemiologia

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may 2017, Volume 20 Suppl. 1 Pages 116 - 128

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1590/1980-5497201700050010 9207 downloads

13.

STAPHYLOCOCCUS AUREUS BIOFILM FORMATION ON POLYPYRROLE: AN ELECTRICAL OVERVIEW
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Cordeiro, Erlon R.

; Fernandes, Antonio W. C.

; Pereira, Alessandra F. C.

; Costa, Mateus M. da

; Nascimento, Marcio L. F.

; Oliveira, Helinando P. de

Química Nova

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sep 2015, Volume 38 N. 8 Pages 1075 - 1079

Abstract: En | Text: En | PDF: En

The development of organic devices based on conducting polymers for biofilm detection requires the combination of superior electrical response and high surface area for biofilm incorporation. Polypyrrole is a potential candidate for application in biofilm detection and control due to its characteristic superior electrical response and strong interaction with bacteria, which enables the use of the bioelectric effect in resulting devices. In this study, chemically synthesized polypyrrole was applied as a support for biofilm growth of S. aureus. Modifications in the electrical response of the polymeric template were explored to identify general mechanisms established during the deposition of the biofilm.

https://doi.org/10.5935/0100-4042.20150112 2218 downloads

14.

Preparation, Characterization, Cytotoxicity and Antioxidant Activity of DOPA Melanin Modified by Amino Acids: Melanin-Like Oligomeric Aggregates
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Costa, Thiago G.

; Feldhaus, Mateus J.

; Vilhena, Felipe S.

; Heller, Melina

; Micke, Gustavo A.

; Oliveira, Aldo S.

; Brighente, Inês M. C.

; Monteiro, Fabiola B. F.

; Creczynski-Pasa, Tânia B.

; Szpoganicz, Bruno

Journal of the Brazilian Chemical Society

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feb 2015, Volume 26 N. 2 Pages 273 - 281

Abstract: En | Text: En | PDF: En

Two new synthetic melanin-like oligomers inspired by the synthesis of pheomelanin (cys-DOPA) were prepared using L-DOPA as a precursor and the amino acids serine (ser-DOPA) and threonine (thr-DOPA) for modifications. The products obtained exhibited appreciable antioxidant activity. The compounds were characterized by liquid chromatography-mass spectrometry (LC-MS), elemental analysis and infrared spectroscopy. The pKa values of the groups present in each melanin suspension were determined in aqueous solution by potentiometric and spectrophotometric titration. Cytotoxicity assays showed an IC50 > 500 mg L-1 resulting in non-toxic compounds for living cells. Finally, cys-DOPA, ser-DOPA and thr-DOPA exhibited antioxidant activity determined by the DPPH scavenging assay, with EC50 = 7.69, 1.02 and 27.60 mg L-1, respectively.

https://doi.org/10.5935/0103-5053.20140277 4451 downloads

15.

II Diretriz de Ressonância Magnética e Tomografia Computadorizada Cardiovascular da Sociedade Brasileira de Cardiologia e do Colégio Brasileiro de Radiologia
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Sara, Leonardo

; Szarf, Gilberto

; Tachibana, Adriano

; Shiozaki, Afonso Akio

; Villa, Alexandre Volney

; Oliveira, Amarino Carvalho de

; Albuquerque, Andrei Skromov de

; Rochitte, Carlos Eduardo

; Nomura, César Higa

; Azevedo, Clerio Francisco

; Jasinowodolinski, Dany

; Tassi, Eduardo Marinho

; Medeiros, Fabio de Morais

; Kay, Fernando Uliana

; Junqueira, Flávia Pegado

; Azevedo, Guilherme S. A.

; Monte, Guilherme Urpia

; Pinto, Ibraim Masciarelli Francisco

; Gottlieb, Ilan

; Andrade, Joalbo

; Lima, João A. C.

; Parga Filho, José Rodrigues

; Kelendjian, Juliana

; Fernandes, Juliano Lara

; Iquizli, Leonardo

; Correia, Luis C. L.

; Quaglia, Luiz Augusto

; Gonçalves, Luiz Flavio Galvão

; Ávila, Luiz Francisco

; Zapparoli, Marcello

; Hadlich, Marcelo

; Nacif, Marcelo Souto

; Barbosa, Márcia de Melo

; Minami, Márcio Hiroshi

; Bittencourt, Marcio Sommer

; Siqueira, Maria Helena Albernaz

; Silva, Marly Conceição

; Lopes, Marly Maria Uellendahl

; Marques, Mateus Diniz

; Vieira, Mônica La Rocca

; Coellho Filho, Otávio Rizzi

; Schvartzman, Paulo R.

; Santos, Raul D.

; Cury, Ricardo C.

; Loureiro, Ricardo

; Cury, Roberto Caldeira

; Sasdelli Neto, Roberto

; Macedo, Robson

; Cerci, Rodrigo Julio

; Faria Filho, Rui Alberto de

; Cardoso, Sávio

; Naves, Thiago

; Magalhães, Tiago Augusto

; Senra, Tiago

; Burgos, Ursula Maria Moreira Costa

; Moreira, Valéria de Melo

; Ishikawa, Walther Yoshiharu

Arquivos Brasileiros de Cardiologia

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dec 2014, Volume 103 N. 6 Pages 1 - 86

Abstract: Pt | Text: Pt | PDF: Pt

https://doi.org/10.5935/abc.2014S006 67586 downloads

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