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au:OLIVEIRA, PAOLA R. DE

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Morphological and biological variations of the glass snake Ophiodes striatus (Spix, 1825) (Squamata, Anguidae) from southeastern Brazil
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OLIVEIRA, PAOLA R. DE

; SANTOS, JULIANE FLORIANO LOPES

; SANTOS, JORGE ABDALA DERGAM DOS

; SOUSA, BERNADETE M. DE

Anais da Academia Brasileira de Ciências

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2021, Volume 93 N. 1 elocation e20190206

Abstract: En | Text: En | PDF: En

Abstract Lizards of the Ophiodes genus, popularly known as glass snakes, are exclusively Neotropical. Despite their wide geographic distribution, glass snakes are poorly studied and there are many knowledge gaps in their biology and ecology to be filled. In this context, the objective of this study was to analyze possible sexual and population morphological differences of Ophiodes striatus specimens collected from different municipalities in the state of Minas Gerais, Brazil. A total of 63 specimens belonging to the Herpetological Collection of the Federal University of Juiz de Fora - Reptiles (CHUFJF-Reptiles), collected between 1995 and 2015 were analyzed. All the specimens were necropsied and morphometric measures related to body and head were measured. The males and females analyzed showed similar morphometric measures, and there was no size-related sexual dimorphism. Principal component analysis did not group the analyzed specimens by sex or locality. A positive correlation was observed between the snout-vent length and the vestigial limb length. The present study brings new information related to sexual dimorphism and population morphometric variation of O. striatus, adding information that will help in better understanding the ecology, biology and evolution of the genus in Brazil.

https://doi.org/10.1590/0001-3765202120190206 527 downloads

Morphological and histological characterization of sexual segment of the kidney in Notomabuya frenata (Cope, 1862) and Aspronema dorsivittatum (Cope, 1862) (Squamata, Mabuyidae)
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NOVELLI, IARA A.

; OLIVEIRA, PAOLA R. DE

; CASTAÑON, MARIA CHRISTINA M.N.

; SILVA, PILAR C.

; SOUSA, BERNADETE M. DE

Anais da Academia Brasileira de Ciências

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aug 2018, Volume 90 N. 2 Pages 2267 - 2278

Abstract: En | Text: En | PDF: En

Abstract The kidneys in two viviparous species of Neotropical lizards, Notomabuya frenata and Aspronema dorsivittatum (Mabuyidae), were investigated by light and scanning electron microscopy in order to determine the presence of the sexual segment of the kidney (SSK) and to study its morphology. The individuals used in this study belong to the Herpetological Collection of the Herpetology Laboratory - Reptiles of the Federal University of Juiz de Fora (CHUFJF-Reptiles) and they were collected between the years 2008 and 2012 from the Cerrado region in the state of Minas Gerais, Brazil. The SSK was present only in sexually mature males (with sperm in the testes / epididymis), whereas it was absent in sexually immature males. The nephron in both species consists of renal corpuscle, proximal convoluted tubule, distal convoluted tubule, collecting duct and sexual segment of the kidney. The SSK of the analyzed species were coated with a simple columnar epithelium, with high cells, basal nucleus and in the apical portion innumerable secretory granules. This study adds to the knowledge on reproductive biology and structures related to reproductive strategies of both lizard species and viviparous Neotropical lizards.

https://doi.org/10.1590/0001-3765201820170871 1115 downloads

Cytopathologic follow-up of women with cervical cancer post-radiotherapy: case series
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Silva, Ruan Carlos G.

; Figueirêdo, Rachel Di Paola V.

; Silva, Amanda Cristina O.

; Lima, Carlos Eduardo Q.

; Oliveira, Sibele R.

; Peres, Adrya Lúcia

Jornal Brasileiro de Patologia e Medicina Laboratorial

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apr 2018, Volume 54 N. 2 Pages 99 - 104

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A citopatologia, além de seu papel nos programas de rastreio do câncer cervical, é uma importante ferramenta para o controle da eficácia do tratamento em mulheres com câncer de colo do útero, monitorando e detectando precocemente neoplasias residuais, recorrentes ou alterações reacionais benignas. Neste trabalho, relatamos oito casos de citopatologia de seguimento em mulheres com câncer de colo do útero após tratamento radioterápico, assistidas por um centro de oncologia pernambucano. Os principais achados citológicos pós-tratamento foram descritos, assim como as características histopatológicas ao diagnóstico e os tratamentos realizados.

ABSTRACT Cytopathology, in addition to its role in cervical cancer screening programs, is also an important tool for controlling the efficacy of treatment in women with cervical cancer by monitoring and early detecting residual, recurrent neoplasms or benign reactive changes. In this study, we report eight cases of cytopathologic follow-up of women with cervical cancer post-radiotherapy, assisted by a Center of Oncology in Pernambuco. The main cytological findings post-treatment were described,as well as the histopathological characteristics at diagnosis and the treatments performed.

https://doi.org/10.5935/1676-2444.20180018 2614 downloads

Determination of Inorganic Contaminants in Electrical and Electronic Equipment after Digestion Using Microwave-Assisted Single Reaction Chamber
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Oliveira, Jussiane S. S.

; Krzyzaniak, Sindy R.

; Picoloto, Rochele S.

; Bolzan, Rodrigo C.

; Mello, Paola A.

Journal of the Brazilian Chemical Society

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sep 2017, Volume 28 N. 9 Pages 1657 - 1664

Abstract: En | Text: En | PDF: En

A method for digestion of plastics from waste of electrical and electronic equipment (WEEE) was developed using the microwave-assisted wet digestion in single reaction chamber (MAWD-SRC). The determination of As, Cd, Co, Cr, Cu, Ni, Pb, Sb and Zn by inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometry (ICP OES) was carried out after sample digestion. Mercury was determined by flow-injection cold vapor generation coupled to inductively coupled plasma mass spectrometry (FI-CVG-ICP-MS). Results obtained using MAWD-SRC for sample preparation were compared with those obtained using microwave-assisted wet digestion (MAWD) at high pressure. Acid mixtures (HNO3 or HNO3 + HCl) were evaluated and feasibility for further inorganic contaminants determination by ICP-MS and ICP OES was demonstrated. Sample preparation by MAWD-SRC using HNO3 + HCl mixture resulted in better digestion efficiency in comparison to MAWD. In addition, lower limits of quantification were obtained using MAWD-SRC due to the higher sample mass that can be digested (500 mg). The combination of HNO3 and HCl for digestion showed to be crucial for quantitative recovery of some elements, as Cr and Sb. Agreement with certified values was better than 96%.

https://doi.org/10.21577/0103-5053.20160317 828 downloads

Bioprospecting Anticancer Compounds from the Marine-Derived Actinobacteria Actinomadura sp. Collected at the Saint Peter and Saint Paul Archipelago (Brazil)
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Silva, Amaro E. T.

; Guimarães, Larissa A.

; Ferreira, Elthon G.

; Torres, Maria da Conceição M.

; Silva, Alison B. da

; Branco, Paola C.

; Oliveira, Francisca Andréa S.

; Silva, Genivaldo G. Z.

; Wilke, Diego V.

; Silveira, Edilberto R.

; Pessoa, Otília Deusdenia L.

; Jimenez, Paula C.

; Costa-Lotufo, Leticia V.

Journal of the Brazilian Chemical Society

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mar 2017, Volume 28 N. 3 Pages 465 - 474

Abstract: En | Text: En | PDF: En

The actinomycete strain BRA 177 was recovered from sediment samples collected at the St. Peter and St. Paul Archipelago, Brazil. This work accessed the ability of this strain, identified as Actinomadura sp., to produce bioactive metabolites by exploring the genome and characterizing chemistry and cytotoxicity of isolated compounds. From the crude ethyl acetate extract, the pigments nonylprodigiosin, cyclononylprodigiosin and methylcyclooctilprodigiosin were isolated and displayed cytotoxicity against human tumor and non-tumor cell lines. Sequencing, assembling and prospection of BRA 177 draft genome led to identification of two contigs encoding enzymes with high homology to those from prodiginine biosynthetic gene clusters (BGC) in actinomycetes. Further, Actinomadura sp. BGC presented unique putatives RedJ thioesterase and RedL-like type I PKS, involved on selection of prodiginine biosynthetic fatty acyl precursor, and RedG-like Rieske oxygenase, key for cyclization of the prodiginines, suggesting that cyclononilprodigiosin and methylcyclooctylprodigiosin could actually be considered chemical signatures of Actinomadura spp.

https://doi.org/10.21577/0103-5053.20160297 1411 downloads

Synthesis of 4-Arylselanylpyrazoles Through Cyclocondensation Reaction Using Glycerol as Solvent
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Nascimento, José E. R.

; Oliveira, Daniela H. de

; Abib, Paola B.

; Alves, Diego

; Perin, Gelson

; Jacob, Raquel G.

Journal of the Brazilian Chemical Society

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aug 2015, Volume 26 N. 8 Pages 1533 - 1541

Abstract: En | Text: En | PDF: En

<p>We describe here a simple method to synthesize 4-arylselanylpyrazoles by reaction of α-arylselanyl-1,3-diketones with arylhydrazines using glycerol as solvent at 60 °C under N2 atmosphere. This is a direct cyclocondensation reaction performed with α-arylselanyl-1,3-diketones and arylhydrazines bearing electron-withdrawing and electron-donating groups affording the corresponding 4-arylselanylpyrazoles in moderate to good yields.</p>

https://doi.org/10.5935/0103-5053.20150127 1475 downloads

Avaliação da sensibilidade e especificidade dos exames não invasivos no diagnóstico da estenose de artéria renal
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Borelli, Flavio Antonio de Oliveira

; Pinto, Ibraim M. F.

; Amodeo, Celso

; Smanio, Paola E. P.

; Kambara, Antonio M.

; Petisco, Ana Claudia G.

; Moreira, Samuel M.

; Paiva, Ricardo Calil

; Lopes, Hugo Belotti

; Sousa, Amanda G. M. R.

Arquivos Brasileiros de Cardiologia

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nov 2013, Volume 101 N. 5 Pages 423 - 433

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

FUNDAMENTO: O envelhecimento e a aterosclerose estão relacionados à hipertensão renovascular em indivíduos idosos. Independentemente das comorbidades, a estenose de artéria renal é, por si só, importante causa de morbidade e mortalidade cardiovascular. OBJETIVO: Definir a sensibilidade, a especificidade, o valor preditivo positivo e o valor preditivo negativo dos exames não invasivos utilizados no diagnóstico de estenose da artéria renal. MÉTODOS: Um grupo de 61 pacientes recrutados permitiram a análise de 122 artérias e a definição de sensibilidade, especificidade e da contribuição relativa de cada exame realizado (Doppler, cintilografia e angiotomografia, comparados a arteriografia renal). RESULTADOS: A média das idades foi de 65,43 (desvio padrão: 8,7) anos. Das variáveis relacionadas à população do estudo e comparadas à arteriografia, duas estiveram correlacionadas à estenose da artéria renal, à disfunção renal e aos triglicerídeos. A mediana do ritmo de filtração glomerular foi de 52,8 mL/min/m². O Doppler identificou sensibilidade de 82,90%, especificidade de 70%, valor preditivo positivo de 85% e valor preditivo negativo de 66,70%. Para a tomografia, encontraram-se sensibilidade de 66,70%, especificidade de 80%, valor preditivo positivo de 87,50% e valor preditivo negativo de 55,20%. Esses achados permitiram identificar os exames que melhor detectavam a estenose. CONCLUSÃO: A tomografia e o Doppler mostraram qualidade e grande possibilidade no diagnóstico de estenose da artéria renal, com vantagem para o segundo, pois não há necessidade do uso de meio de contraste na avaliação de uma doença que, frequentemente, ocorre em diabéticos e associa-se à disfunção renal e à disfunção ventricular esquerda grave.

BACKGROUND: Aging and atherosclerosis are related to renovascular hypertension in elderly individuals. Regardless of comorbidities, renal artery stenosis is itself an important cause of cardiovascular morbidity and mortality. OBJECTIVE: To define the sensitivity, specificity, positive predictive value, and negative predictive value of noninvasive imaging tests used in the diagnosis of renal artery stenosis. METHODS: In a group of 61 patients recruited, 122 arteries were analized, thus permitting the definition of sensitivity, specificity, and the relative contribution of each imaging study performed (Doppler, scintigraphy and computed tomographic angiography in comparison to renal arteriography). RESULTS: The mean age was 65.43 years (standard deviation: 8.7). Of the variables related to the study population that were compared to arteriography, two correlated with renal artery stenosis, renal dysfunction and triglycerides. The median glomerular filtration rate was 52.8 mL/min/m². Doppler showed sensitivity of 82.90%, specificity of 70%, a positive predictive value of 85% and negative predictive value of 66.70%. For tomography, sensitivity was 66.70%, specificity 80%, positive predictive value 87.50% and negative predictive value 55.20%. With these findings, we could identify the imaging tests that best detected stenosis. CONCLUSION: Tomography and Doppler showed good quality and efficacy in the diagnosis of renal artery stenosis, with Doppler having the advantage of not requiring the use of contrast medium for the assessment of a disease that is common in diabetics and is associated with renal dysfunction and severe left ventricular dysfunction.

https://doi.org/10.5935/abc.20130191 4466 downloads

Consenso de imunização para crianças e adolescentes com doenças reumatológicas
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Silva, Clovis Artur A

; Terreri, Maria Teresa R. A

; Barbosa, Cássia Maria P.L

; Hilário, Maria Odete E

; Pillegi, Gecilmara C. S

; Ferriani, Virginia P.L

; Aikawa, Nadia E

; Carvalho, Jozélio F

; Jesus, Adriana A

; Sallum, Adriana M. E

; Lotito, Ana Paola N

; Liphaus, Bernadete L

; Magalhães, Claudia S

; Len, Cláudio A

; Okuda, Eunice M

; Campos, Lucia Maria M

; Carvalho, Luciana M

; Ronchezel, Marcos Vinícius

; Santos, Maria Carolina dos

; Romanelli, Paulo Roberto S

; Marini, Roberto

; Pereira, Rosa Maria R

; Sacchetti, Silvana B

; Lotufo, Simone

; Hirschheimer, Sônia Maria S

; Robazzi, Tereza Cristina M.V

; Vecchi, Ana Paula

; Moraes, Ana Júlia P

; Naka, Erica N

; Bica, Blanca

; Oliveira, Sheila K.F

; Bastos, Wanda A

Revista Brasileira de Reumatologia

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oct 2009, Volume 49 N. 5 Pages 562 - 589

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Crianças e adolescentes com doenças reumatológicas apresentam maior prevalência de doenças infecciosas quando comparados com a população em geral, em decorrência de atividade da doença, possível deficiência imunológica secundária à própria doença, ou uso de terapia imunossupressora. A vacinação é uma medida eficaz para a redução da morbidade e mortalidade nesses pacientes. O objetivo deste artigo foi realizar um consenso de eficácia e segurança das vacinas em crianças e adolescentes com doenças reumatológicas infantis baseadas em níveis de evidência científica. Imunização passiva para os pacientes e orientações para as pessoas que convivem com doentes imunodeprimidos também foram incluídas. Os 32 pediatras reumatologistas membros do Departamento de Reumatologia da Sociedade de Pediatria de São Paulo (SPSP) e/ou da Comissão de Reumatologia Pediátrica da Sociedade Brasileira de Reumatologia elaboraram o consenso, sendo que alguns desses profissionais estão envolvidos em pesquisas e publicações científicas nesta área. A pesquisa dos termos eficácia e/ou segurança das diferentes vacinas em crianças e adolescentes com doenças reumatológicas foi realizada nas bases de Medline e Scielo, de 1966 até março de 2009, incluindo revisões, estudos controlados e relatos de casos. O grau de recomendação e o nível científico de evidências dos estudos foram classificados em quatro níveis para cada vacina. De um modo geral, as vacinas inativadas e de componentes são seguras nos pacientes com doenças reumatológicas, mesmo em uso de terapias imunossupressoras. Entretanto, vacinas com agentes vivos atenuados são, em geral, contraindicadas para os pacientes imunossuprimidos.

Incidence of infectious diseases is higher in children and adolescents with rheumatic diseases than in the general population due to disease activity, possible immune deficiency secondary to the disease itself, or the use of immunosuppressive drugs. Vaccination is effective in reducing morbidity and mortality in those patients. The objective of this study was to establish an evidence-based consensus on the efficacy and safety of vaccination in children and adolescents with rheumatic diseases. Passive immunization of patients and guidelines for people who live with immunosuppressed patients were also included. The 32 pediatric rheumatologists of the Rheumatology Department of the Pediatrics Society of São Paulo, (SPSP, from the Portuguese), São Paulo, SP, Brazil, and/or the Commission on Pediatrics Rheumatology of the Brazilian Society of Rheumatology are responsible for this consensus; some of those professionals are involved on research and scientific publications in this field. The words efficacy and/or safety of different vaccines in children and adolescents with rheumatologic diseases were searched in Medline and Scielo data bases from 1966 to March 2009, including reviews, controlled studies, and case reports. The degree of recommendation and the scientific evidence of the studies were classified in four levels for each vaccine. As a rule, inactive and protein components vaccines are safe for patients with rheumatologic diseases, even in the presence of immunosuppressive therapy. However, live attenuated vaccines are, in general, contraindicated for immunosuppressed patients.

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Fatores biológicos e superestimação da fração de ejeção do ventrículo esquerdo no gated SPECT
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Oliveira, Marco Antônio Condé de

; Duarte, Paulo Schiavom

; Gonzalez, Maria Margarita C.

; Moises, Valdir Ambrósio

; Alonso, Gilberto

; Lima, Eduardo Vilaça

; Smanio, Paola Emanuela

; Martins, Luiz Roberto Fernandes

; Oliveira, Carlos A. R.

; Mastrocolla, Luiz Eduardo

Arquivos Brasileiros de Cardiologia

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may 2008, Volume 90 N. 5 Pages 333 - 338

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

FUNDAMENTO: Alguns pacientes apresentam fração de ejeção do ventrículo esquerdo (FEVE) superestimada na cintilografia miocárdica com sincronização eletrocardiográfica (gated SPECT). OBJETIVO: Estabelecer a relação entre fatores biológicos e FEVE superestimada. MÉTODOS: Selecionamos 3.838 pacientes que realizaram gated SPECT entre 20/5/2000 e 16/9/2005, com imagens normais de perfusão e FEVE >50%. Analisamos as variáveis: sexo (29,4% feminino e 70,6% masculino), idade (de 20 a 94 anos - média: 56 anos), peso (de 33,5 a 150 kg - média: 79,6 kg), altura (de 138 a 220 cm - média: 171 cm) e IMC (de 13,9 a 54 - média: 27,2). Em um subgrupo de 1.002 pacientes que realizaram ecocardiograma, incluímos as variáveis diâmetros diastólico (de 36 a 68 mm - média 47,5 mm) e sistólico (de 22 a 41 mm - média 29,8 mm). Dividimos os pacientes em dois grupos: FEVE normal (<80%) e superestimada (>80%). A Razão de Chances (RC) para apresentar FEVE superestimada foi calculada para cada variável por regressão logística. RESULTADOS: Encontramos as seguintes Razões de Chances (p < 0,005): sexo feminino RC = 3,585 (IC95%: 2,745 a 4,683), idade em anos RC = 1,020 (IC95%: 1,011 a 1,029) e altura em cm RC = 0,893 (IC95%: 0,829 a 0,962). O peso e o IMC não se associaram a FEVE significativamente (p>0,2). No subgrupo de 1.002 pacientes, encontramos influência estatisticamente significativa na obtenção da FEVE superestimada para as variáveis diâmetro sistólico, sexo e altura. CONCLUSÃO: Apesar de o diâmetro sistólico influenciar na obtenção da FEVE superestimada, as variáveis sexo e altura apresentam influência independente na superestimação da FEVE pelo gated SPECT.

BACKGROUND: Some patients present an overestimated left ventricular ejection fraction (LVEF) on electrocardiogram-gated myocardial scintigraphy (gated SPECT). OBJECTIVE: To establish the relationship between biological factors and overestimated LVEF. METHODS: We selected 3838 patients who underwent gated SPECT between May 20, 2000 and September 16, 2005 with normal perfusion images and LVEF >50%. The following variables were analyzed: gender (29.4% females and 70.6% males), age (from 20 to 94 years - mean: 56 years), weight (from 33.5 to 150 kg - mean: 79.6 kg), height (from 138 to 220 cm - mean: 171 cm) and BMI (from 13.9 to 54 - mean: 27.2). In a subgroup of 1002 patients who underwent echocardiogram, the diastolic diameter (from 36 to 68 mm - mean 47.5 mm) and systolic diameter (from 22 to 41 mm - mean 29.8 mm) variables were included. The patients were divided into two groups: normal LVEF (<80%) and overestimated LVEF (>80%). The odds ratio (OR) for presenting an overestimated LVEF was calculated for each variable using logistic regression. RESULTS: The following odds ratios were found (p < 0.005): female gender OR = 3.585 (95%CI: 2.745 to 4.683), age in years OR = 1.020 (95%CI: 1.011 to 1.029) and height in cm OR = 0.893 (95%CI: 0.829 to 0.962). Weight and BMI were not significantly associated with LVEF (p>0.2). In the subgroup of 1002 patients, a statistically significant influence was found in overestimated LVEF values for the systolic diameter, gender and height variables. CONCLUSION: Although systolic diameter influences the overestimation of LVEF, the gender and height variables have an independent influence on LVEF overestimation by gated SPECT.

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