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Abstract The objective of this work was to evaluate the effects of additives and storage time on the quality and aerobic deterioration of silages of pineapple crop waste. A completely randomized design was used, in a 3×3 factorial arrangement, with three treatments: pineapple waste silage without additives or with the addition of cornmeal or wheat bran (20% fresh matter) at three storage times (60, 90, and 120 days). After each storage time, losses and aerobic deterioration were quantified, chemical composition was analyzed, and digestibility assays were performed. The storage time of 120 days resulted in higher dry matter losses in all treatments. The additives incremented the dry matter contents of the pineapple-based silages, which went from 182.1 g kg−1 (control silage) to 298.7 and 297.6 g kg−1 (cornmeal and wheat bran, respectively). The control silage with the addition of cornmeal showed the highest dry matter digestibility (739.7 g kg−1) and lowest neutral detergent fiber content (251.9 g kg−1). The production of CO2 started to increase on the first day of air exposure in silages without additives, but only after six days in those with additives. Including cornmeal as an additive in the silage of pineapple crop waste improves its digestibility and reduces its deterioration speed.

Resumo O objetivo deste trabalho foi avaliar os efeitos de aditivos e tempos de armazenamento sobre a qualidade e a deterioração aeróbia de silagens de resíduos de cultura do abacaxi. Utilizou-se um delineamento inteiramente casualizado, em arranjo fatorial 3×3, com três tratamentos: silagem de resíduos culturais de abacaxi sem aditivo ou aditivada com fubá de milho ou farelo de trigo (20% da matéria fresca), em três tempos de armazenamento (60, 90 e 120 dias). Após cada tempo de armazenamento, realizaram-se quantificações de perdas e deterioração aeróbia, análises da composição química e ensaios de digestibilidade. O tempo de armazenamento por 120 dias resultou em maiores perdas de matéria seca em todos os tratamentos. Os aditivos incrementaram os teores de matéria seca das silagens à base de abacaxi, que passaram de 182,1 g kg−1 (silagem controle) para 298,7 e 297,6 g kg−1 (fubá de milho e farelo de trigo, respectivamente). A silagem controle adicionada de fubá de milho apresentou maior digestibilidade da matéria seca (739,7 g kg−1) e menor teor de fibra em detergente neutro (251,9 g kg−1). A produção de CO2 aumentou a partir do primeiro dia de exposição ao ar nas silagens não aditivadas, mas apenas a partir do sexto dia nas aditivadas. A inclusão do fubá de milho como aditivo na silagem de resíduos de abacaxi melhora sua digestibilidade e reduz sua velocidade de deterioração.

https://doi.org/10.1590/s1678-3921.pab2023.v58.03211

Effect of pond fertilization on growth performance of pirarucu (Arapaima gigas) during grow-out phase
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Pereira-Rezende, Fabricio

; Ferreira-Lima, Adriana

Latin american journal of aquatic research

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mar 2022, Volume 50 N. 1 Pages 22 - 30

Abstract: En | Text: En | PDF: En

ABSTRACT. Fertilization of aquaculture ponds has been pointed out as an efficient practice to improve fish feed conversion and growth performance. In this way, the present study aimed to assess the effect of pond fertilization on the growth performance, body composition, and water quality of juvenile pirarucu Arapaima gigas reared in earthen ponds (600 m2) during the grow-out phase. Two fertilized and two non-fertilized ponds were stocked with 208 juvenile pirarucus (19.9 ± 3.6 g and 15.0 ± 0.9 cm) and reared for 100 days. All four ponds were limed before the study. Ponds were fertilized before fish were stocked and weekly until the end of the experiment, and fish offered feed until apparent satiation. Pirarucu reared in fertilized ponds presented higher final weight (20%) and biomass (19%), further to lower feed intake and feed conversion rate (1.31) than fish reared in non-fertilized ponds (1.61). Pond fertilization did not affect the fish coefficient of variation, which was reduced over the culture cycle. The body composition of fish reared in fertilized ponds presented higher water content, protein, and energy retention rates and lowered crude fat and energy. Fertilized ponds presented lower transparency levels and higher pH from the 50th day of culture, whereas ammonia concentration was higher in the fertilized ponds in the first 25 days. Therefore, pond fertilization is a recommended practice for pirarucu during the grow-out phase.

https://doi.org/10.3856/vol50-issue1-fulltext-2617 26 downloads

Association of TAP1 1177A>G and 2090A>G gene polymorphisms with latent tuberculosis infections in sheltered populations, in the metropolitan area of Guadalajara, Mexico: a pilot study
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Cazarez-Navarro, Gerardo

; Palomares-Marín, Jaime

; Rodríguez-Preciado, Sergio Yair

; Pereira-Suárez, Ana Laura

; Martínez-López, Erika

; Bacilio-Medrano, Eva Adriana

; Huerta-Olvera, Selene

; Hernández-Cañaveral, Iván Isidro

Revista do Instituto de Medicina Tropical de São Paulo

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2021, Volume 63 elocation e55

Abstract: En | Text: En | PDF: En

ABSTRACT Latent tuberculosis infection (LTBI) is a condition that has no clinical signs and symptoms. LTBI patients are characterized by persistent immune responses to Mycobacterium tuberculosis, and approximately 5-10% of these infected individuals will develop active TB at some point in their lives. The antigen transporter associated with antigen processing (TAP1) is a protein involved in the transport of the antigen from the cytoplasm to the endoplasmic reticulum by means of the association with MHC class I molecules. It plays a fundamental role in the immune response, promoting the clearance of intracellular pathogens. Our pilot study aimed to determine the association between TAP1 gene 1177A>G (rs1057141) and 2090A>G (rs1135216) genetic polymorphisms with susceptibility to LTBI. In this case-control study, 153 individuals from shelters were analyzed (46 were LTBI-positive and 92 were controls). Genotyping of the rs11352216 (2090A>G) and rs1057141 (1177A>G) gene IDs was performed using the Applied Biosystems Step One Thermal Cycler Real-Time PCR allelic discrimination technology. The haplotypic analyses were performed with the Arlequin 3.5 program. Social assistance centers and shelters that serve vulnerable populations represent high-risk sites due to overcrowding and the impaired nutritional status of their residents. The G allele (OR=1.99, CI=1.109-3.587, p=0.021) and the GG genotype of rs11352216 (A>G) were associated with susceptibility to LTBI, according to the codominant genetic model (OR=8.32, CI=1.722-61.98, p=0.007). The rs1057141 (A>G) polymorphism was not associated with LTBI risk. The results suggest that carriers of the G allele of rs1135216 (A>G) are susceptible to LTBI.

https://doi.org/10.1590/s1678-9946202163055 153 downloads

Effect of density of fingerling and juvenile pirarucu during transportation on water quality and physiological parameters
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LIMA, Adriana Ferreira

; OLIVEIRA, Hyago Jovane Borges de

; PEREIRA, André Silvério

; SAKAMOTO, Silmara Sanae

Acta Amazonica

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sep 2020, Volume 50 N. 3 Pages 223 - 231

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Este estudo avaliou o efeito da densidade de estocagem sobre parâmetros fisiológicos (lactato, glicose, cortisol e hematócrito), qualidade de água (temperatura, oxigênio dissolvido, pH, amônia não ionizada e gás carbônico) e sobrevivência no transporte de alevinos (24.5 ± 4.7 g) e juvenis (615.83 ± 122.19 g) de pirarucu (Arapaima gigas) em sacos plásticos por seis horas. Avaliamos densidades de 65, 80, 95, 110 e 125 g L-1 para alevinos, e de 50, 80, 110, 140 e 170 g L-1 para juvenis (três réplicas cada). Os parâmetros de resposta foram medidos antes e logo após o transporte, e após 24 e 96 horas de recuperação. Não foi observada mortalidade, exceto para alevinos (< 3%) das densidades de 110 e 125 g L-1 durante o período de recuperação. Foram observadas alterações significativas em todos os parâmetros de qualidade de água após o transporte de alevinos e juvenis. Houve elevação da temperatura, oxigênio dissolvido, gás carbônico e amônia não ionizada e decréscimo do pH, com diferenças entre as densidades apenas para gás carbônico e amônia não ionizada. Não houve elevação dos níveis de cortisol, exceto para juvenis na densidade de 170 g L-1 que ainda permaneciam com nível alto de cortisol às 96 horas. A glicose se elevou após o transporte em todos os tratamentos, retornando aos valores iniciais na recuperação, ao contrário do lactato, que se elevou e permaneceu alto até as 96 horas. O hematócrito foi avaliado apenas para juvenis e diminuiu significativamente após o transporte. Concluímos que alevinos de pirarucu podem ser transportados com segurança em densidades de até 95 g L-1 e juvenis em densidades de até 140 g L-1.

ABSTRACT We assessed the effect of stocking density on physiological parameters (blood lactate, glucose, cortisol, hematocrit), water quality (temperature, dissolved oxygen, pH, unionized ammonia, carbon dioxide), and survival during the transportation of fingerling (24.5 ± 4.7 g) and juvenile (615.8 ± 122.2 g) pirarucu (Arapaima gigas) for six hours in plastic bags. The tested densities were 65, 80, 95, 110 and 125 g L-1 for fingerlings, and 50, 80, 110, 140 and 170 g L-1 for juveniles (three replicates each). Parameters were measured prior to and immediately after transportation, and at 24 and 96 hours recovery after transportation. No mortality was observed, except for fingerlings (< 3%) at densities of 110 and 125 g L-1 during recovery. All the water quality parameters were significantly altered after the transportation of fingerlings and juveniles. Water temperature, dissolved oxygen, carbon dioxide and unionized ammonia increased, but pH decreased. Only carbon dioxide and unionized ammonia differed among densities. Cortisol levels did not increase over time, except for the juveniles at 170 g L-1, which still had high cortisol after 96 hours. Glucose significantly increased after transportation for all the treatments and returned to the initial values during the recovery period. Conversely, the lactate values were still high after 96 hours. Hematocrit was assessed only for juveniles and was significantly lower after transportation. We conclude that fingerling and juvenile pirarucu can be safely transported at densities up to 95 g L-1 and 140 g L-1, respectively.

https://doi.org/10.1590/1809-4392202000302 1027 downloads

Maltose in culture media improves the in vitro regeneration of Urochloa brizantha cv. ‘Marandu’ plants
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Carrion Pereira, Alyne Valéria

; Midori Takamori, Luciana

; Harumi Yaguinuma, Diliane

; Mendonça de Oliveira, Adriana

; Ferreira Ribas, Alessandra

Biotecnología Vegetal

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sep 2019, Volume 19 N. 3 Pages 205 - 213

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT The improvement of the in vitro regeneration of Urochloa brizantha is an important step to toward the development of transgenic cultivars. The objective of this work was to test the effect of the amino acid proline and the replacement of sucrose by maltose as carbon source in culture media to optimize the somatic embryogenesis. Mature seeds of U. brizantha cv. ‘Marandu’ were used as initial explant. The seeds were scarified in sulfuric acid, manually peeled disinfested and washed. The callus induction media were composed basically by Murashige and Skoog salts supplemented with 30 g l-1 sucrose, 3 mg l-1 2,4-dichlorophenoxy acetic acid, 300 mg l-1 hydrolyzed casein and 8 g l-1 agar. Carbon sources maltose or sucrose at 30, 40 and 50 g l-1 was added in the callus induction medium. In another experiment the L-proline was added to the media at 100, 200 and 400 mg l-1. It was evaluated the number of seeds that produced callus, calluses growth after 14 weeks (mg) and number of regenerated plants. The results showed maltose was more efficient than sucrose for regenerate the plants. Nevertheless, the addition of proline to the media not improved it. Therefore, substitution of sucrose by maltose to the in vitro culture media of U. brizantha increase the plant regeneration.

RESUMEN La mejora de la regeneración in vitro de Urochloa brizantha es un paso importante hacia el desarrollo de cultivares transgénicos. El objetivo de este trabajo fue evaluar el efecto del aminoácido prolina y el reemplazo de sacarosa por maltosa como fuente de carbono en medios de cultivo para optimizar la embriogénesis somática. Semillas maduras de U. brizantha cv. ‘Marandu’ fueron utilizados como explante inicial. Las semillas fueron escarificadas en ácido sulfúrico, peladas manualmente, desinfectadas y lavadas. Los medios de cultivo para la formación de callos estuvieron compuestos básicamente por sales de Murashige y Skoog con 30 g l-1 de sacarosa, 3 mg l-1 de ácido 2,4-diclorofenoxi acético, 300 mg l-1 de caseína hidrolizada y 8 g l-1 de agar. Se añadieron fuentes de carbono maltosa o sacarosa a 30, 40 y 50 g l-1 en el medio de cultivo de formación de callos. En otro experimento, se añadió L-prolina a los medios de cultivo a 100, 200 y 400 mg l-1. Se evaluó el número de semillas que produjeron callos, el crecimiento de callos después de 14 semanas (mg) y el número de plantas regeneradas. Los resultados mostraron que la maltosa era más eficiente que la sacarosa para regenerar las plantas. La adición de prolina a los medios de cultivo no lo mejoró. Por lo tanto, la sustitución de sacarosa por maltosa en los medios de cultivo in vitro de U. brizantha aumenta la regeneración de las plantas.

Fluxos de óxido nitroso e suas relações com atributos físicos e químicos do solo
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Corrêa, Rubia Santos

; Madari, Beáta Emöke

; Carvalho, Glaucilene Duarte

; Costa, Adriana Rodolfo da

; Pereira, Ana Cláudia de Castro

; Medeiros, João Carlos

Pesquisa Agropecuária Brasileira

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sep 2016, Volume 51 N. 9 Pages 1148 - 1155

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O objetivo deste trabalho foi avaliar a dinâmica dos fluxos de óxido nitroso (N2O) em solo sob pastagem, em sistema de integração lavoura-pecuária, e sob cerrado nativo usado como referência, bem como identificar os atributos físicos e químicos do solo que influenciam esses fluxos. O estudo foi realizado na Fazenda Capivara, da Embrapa Arroz e Feijão, de 5 de fevereiro a 30 de setembro de 2013. As amostragens de gás foram realizadas com uso de câmaras estáticas manuais. O solo sob pastagem apresentou os maiores fluxos de N2O, com média de 14,12 ng m-2 s-1. Essa área também apresentou maior espaço poroso preenchido por água (EPPA), que, associado à temperatura e ao teor de nitrato no solo, influenciou a produção de N2O. Na época chuvosa, os fluxos apresentaram correlação positiva com o EPPA do solo e, na época seca, com seu teor de amônio e nitrato. A emissão total de N2O na área da pastagem foi de 1.644,19 gha1. No cerrado nativo, não houve emissão de N2O do solo para a atmosfera.

Abstract The objective of this work was to evaluate the dynamics of nitrous oxide (N2O) fluxes in a soil under pasture, in an integrated crop-livestock system, and in native cerrado as a reference, besides identifying the physical and chemical soil properties that affect the fluxes. The study was conducted at Fazenda Capivara of Embrapa Arroz e Feijão, in the state of Goiás, Brazil, between February 5 and September 30, 2013. Gas sampling was performed using manual static chambers. The soil under pasture showed the highest N2O fluxes with average of 14.12 ng m-2 s-1. This area also showed greater water-filled pore space (WFPS), which, associated with soil temperature and nitrate content in the soil affected the production of N2O. In the rainy season, fluxes showed positive correlation with soil WFPS, and, in the dry season, they were correlated with soil contents of ammonium and nitrate. The total N2O emission in the pasture area was 1,644.19 g ha-1. In native cerrado, there was no soil-N2O emission to the atmosphere.

https://doi.org/10.1590/s0100-204x2016000900014 1718 downloads

Factors associated with the need for ventilation at birth of neonates weighing ≥2,500 g
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Sousa, José Roberto Pereira de

; Leite, Álvaro Jorge Madeiro

; Sanudo, Adriana

; Guinsburg, Ruth

Clinics

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jul 2016, Volume 71 N. 7 Pages 381 - 386

Abstract: En | Text: En | PDF: En

OBJECTIVES: Approximately 20-40% of annual global neonatal deaths occur among infants with birthweights ≥2,500 g, and most of these deaths are associated with intrapartum asphyxia in low- and middle-income countries. This study aims to evaluate the peripartum variables associated with the need for resuscitation at birth of neonates weighing ≥2,500 g. METHOD: This case-control retrospective study was performed on data from all public reference maternity units in the state of Ceará, Northeast Brazil, between March 2009 and March 2010. The subjects were singleton neonates without malformations weighing ≥2,500 g, who required positive-pressure ventilation in the delivery room. The controls had a 1-minute Apgar score of ≥8 and did not undergo resuscitation. Variables associated with positive-pressure ventilation in the delivery room were evaluated via conditional multivariate logistic regression. RESULTS: Of the 2,233 live births with birth weights ≥2,500 g, 1-minute Apgar scores ≤7, and no malformations, 402 patients met the inclusion criteria, and they were paired with 402 controls. Risk variables for positive-pressure ventilation at birth were a gestational age <37 weeks (OR: 3.54; 95% CI: 1.14-10.92) and meconium-stained amniotic fluid (8.53; 4.17-17.47). Cervical examination at maternal admission (0.57; 0.38-0.84) and a written follow-up of the labor (0.68; 0.46-0.98) were identified as protective variables. CONCLUSIONS: Significant flaws in obstetric care are associated with the need for positive-pressure ventilation at birth for neonates weighing ≥2,500 g.

https://doi.org/10.6061/clinics/2016(07)05 797 downloads

Levels of feed supplementation on the qualitative aspects of meat from crossbred goats finished on caatinga
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Silva, Daniel Cézar da

; Guim, Adriana

; Santos, Gladston Rafael de Arruda

; Maciel, Maria Inês Sucupira

; Soares, Luciana Felizardo Pereira

Revista Ciência Agronômica

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dec 2015, Volume 46 N. 4 Pages 855 - 864

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMOAvaliou-se o efeito da suplementação alimentar (0; 0,4; 0,8 e 1,2% do peso corporal) sobre os aspectos qualitativos da carne caprina (umidade, cinzas, gordura, proteína, coloração da carne pelo sistema CIE (L*, a* e b*), pH, perda por cocção e força de cisalhamento) e atributos sensoriais aroma caprino, aroma estranho, cor, textura, maciez, sabor caprino, sabor estranho, suculência e aparência geral. Utilizou-se 32 caprinos mestiços da raça Anglonubiana que foram distribuídos segundo delineamento em blocos casualizados, sendo terminados pastagem de caatinga. Houve efeito quadrático (p<0,05) para os teores de cinza e proteína, com máxima de 1,06 e 20,44 g/100 g, respectivamente. Verificou-se efeito linear positivo (p<0,05) sobre o teor de gordura, com incremento de 0,52 g/100 g. Os parâmetros qualitativos foram influenciados (p<0,05), com efeito linear negativo (p<0,05) sobre a perda por cocção, força de cisalhamento e L*, com decréscimos de 3,32%; 0,77 kgf/cm2 e 3,39, respectivamente, e efeito quadrático (p<0,05) para a*, com máxima de 9,13. O parâmetro b* apresentou efeito quadrático (p<0,05), com máxima 8,12, ao contrário do pH que não foi influenciado, com média 5,57. Os atributos sensoriais não foram influenciados (p>0,05) pela suplementação alimentar, com exceção da cor e aparência geral. A suplementação alimentar com 0,8% do peso corporal propicia elevação nos teores de cinza, gordura e proteína, associados a melhoras qualitativas sobre a perda por cocção, maciez e coloração, com incrementos dos atributos sensoriais de cor e aparência geral.

ABSTRACTThe effect was evaluated of feed supplementation (0.0, 0.4, 0.8, and 1.2% of body weight) on the qualitative aspects of goat meat (moisture, ash, fat, protein, colouring using the CIE (L*, a* and b *) system, pH, cooking loss and shear force), and also on the sensory attributes of goat aroma, off-aroma, colour, texture, tenderness, flavour, off-flavour, juiciness and overall appearance. A total of 32 crossbred Anglo-Nubian goats were used, organised into randomised blocks and finished on a pasture of caatinga. There was a quadratic effect (p<0.05) on the levels of ash and protein, with a maximum of 1.06 and 20.44 g/100 g respectively. A positive linear affect (p<0.05) was seen on fat levels, with an increase of 0.52 g/100 g. There was a negative linear effect (p<0.05) on the qualitative parameters of cooking loss, shear force and L*, with decreases of 3.32%, 0.77 kgf/cm2 and 3.39 respectively, and a quadratic effect (p<0.05) on a*, with a maximum of 9.13. There was a quadratic effect (p<0.05) on the parameter b*, with a maximum of 8.12, while the pH was not affected, with an average of 5.57. The sensory attributes were not influenced (p>0.05) by feed supplementation, except for colour and general appearance. Feed supplementation at 0.8% of body weight provides an increase in ash, fat and protein levels associated with qualitative improvements in cooking loss, tenderness and colouring, with increases in the sensory attributes of colour and overall appearance.

https://doi.org/10.5935/1806-6690.20150074 1149 downloads

Lisina digestível em dietas para suínos machos castrados de alto potencial genético para deposição de carne magra na carcaça dos 60 aos 95 kg
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Oliveira, Alexandre Luiz Siqueira de

; Donzele, Juarez Lopes

; Silva, Francisco Carlos de Oliveira

; Oliveira, Rita Flávia Miranda de

; Abreu, Márvio Lobão Teixeira de

; Pereira, Adriana Aparecida

; Scottá, Bruno Andreatta

Revista Brasileira de Saúde e Produção Animal

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dec 2014, Volume 15 N. 4 Pages 983 - 993

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Foram utilizados 50 suínos machos castrados, com alto potencial genético para deposição de carne magra na carcaça e peso inicial de 60,5 ± 1,58 kg para avaliar diferentes níveis de lisina digestível sobre o desempenho e as características de carcaça. Foi usado o delineamento de blocos ao acaso, com cinco tratamentos, cinco repetições e dois animais por repetição. Os tratamentos consistiram de cinco níveis de lisina digestível (0,600; 0,700; 0,800; 0,900 e 1,000% de lisina) obtidos variando-se a proporção de milho e farelo de soja nas rações. Os animais permaneceram no experimento até atingirem o peso final de 95,8 ± 2,64 kg. Os níveis de lisina influenciaram de forma quadrática a conversão alimentar e a conversão alimentar em músculo, que melhoraram até o nível de 0,85% de lisina digestível, e o rendimento de carne magra, que aumentou até o nível de 0,840% de lisina digestível. O consumo de lisina diário aumentou de forma linear em razão dos níveis de lisina da ração. Não houve efeito dos tratamentos sobre o ganho de peso, consumo de ração, características de carcaça, rendimento de carcaça, rendimento de gordura e rendimento de pernil. Conclui-se que suínos machos castrados, dos 60 aos 95 kg, exigem 0,850% de lisina digestível, correspondente a um consumo diário estimado de 24,2 g de lisina digestível.

Fifty barrows with high genetic potential for lean gain and 60.5 ± 1.58kg of average initial live weight were used in a study to evaluate different lysine levels on performance and carcass traits. A randomized blocks experimental design, with five treatments, five replicates and two animals per replicate was used. Treatments consisted in five digestible lysine levels (0.600; 0.700; 0.800; 0.900; e 1.000%) obtained with the variation of corn and soybean meal proportion. Animals were kept in the experiment until they reached 95.8 + 2.64kg of final live weight. Lysine levels had a quadratic effect on feed conversion and feed conversion in muscle, which improved to the level of 0.85 % digestible lysine , and lean meat yield , which increased up to 0.840 % digestible lysine.The daily lysine intake increased linearly with the elevation of dietary levels of lysine. There was no effect of treatments on weight gain, feed intake, carcass traits, carcass yield, fat yield, and ham yield. It was concluded that barrows from 60 to 95 kg require 0.850% of total lysine, corresponding to a digestible lysine estimated intake of 24.2 g/day.

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Perfil epidemiológico da espondiloartrite de início juvenil comparada com a espondiloartrite de início na vida adulta em uma grande coorte brasileira
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Duarte, Angela P

; Marques, Cláudia DL

; Bortoluzzo, Adriana B

; Gonçalves, Célio R

; Silva, José Antonio Braga da

; Ximenes, Antonio Carlos

; Bértolo, Manoel B

; Ribeiro, Sandra Lúcia E

; Keiserman, Mauro

; Skare, Thelma L

; Carneiro, Sueli

; Menin, Rita

; Azevedo, Valderilio F

; Vieira, Walber P

; Albuquerque, Elisa N

; Bianchi, Washington A

; Bonfiglioli, Rubens

; Campanholo, Cristiano

; Carvalho, Hellen MS

; Costa, Izaias P

; Kohem, Charles L

; Leite, Nocy

; Lima, Sonia AL

; Meirelles, Eduardo S

; Pereira, Ivânio A

; Pinheiro, Marcelo M

; Polito, Elizandra

; Resende, Gustavo G

; Rocha, Francisco Airton C

; Santiago, Mittermayer B

; Sauma, Maria de Fátima LC

; Valim, Valéria

; Barros, Percival D Sampaio

Revista Brasileira de Reumatologia

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dec 2014, Volume 54 N. 6 Pages 424 - 430

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Objetivo Analisar as características clínicas e epidemiológicas das espondiloartrites (EpA) de início juvenil (< 16 anos) e compará-las com um grupo de pacientes com EspA de início na vida adulta (≥ 16 anos). Pacientes e métodos Coorte prospectiva, observacional e multicêntrica com 1.424 pacientes com diagnóstico de EspA de acordo com o European Spondyloarthropathy Study Group (ESSG) submetidos a um protocolo comum de investigação e recrutados em 29 centros de referência participantes do Registro Brasileiro de Espondiloartrites (RBE). Os pacientes foram divididos em dois grupos: idade no início<16 anos (grupo EspAiJ) e idade no início ≥ 16 anos. Resultados Entre os 1.424 pacientes, 235 manifestaram o início da doença antes dos 16 anos (16,5%). As variáveis clínicas e epidemiológicas associadas com a EspAiJ foram: gênero masculino (p<0,001), artrite em membro inferior (p=0,001), entesite (p=0,008), uveíte anterior (p=0,041) e HLA-B27 positivo (p=0,017), em associação com escores mais baixos de atividade da doença (Bath Ankylosing Spondylitis Disease Activity Index – BASDAI; p=0,007) e de capacidade funcional (Bath Ankylosing Spondylitis Functional Index – BASFI; p=0,036). A psoríase cutânea (p<0,001), a doença inflamatória intestinal (p=0,023), a dactilite (p=0,024) e o envolvimento ungueal (p=0,004) foram mais frequentes em pacientes com EspA de início na vida adulta. Conclusões Nessa grande coorte brasileira, os pacientes com EspAiJ se caracterizavam predominantemente pelo gênero masculino, envolvimento periférico (artrite e entesite), HLA-B27 positivo e escores de doença mais baixos.

Objective To analyze the clinical and epidemiologic characteristics of juvenile-onset spondyloarthritis (SpA) (< 16 years) and compare them with a group of adult-onset (≥ 16 years) SpA patients. Patients and methods Prospective, observational and multicentric cohort with 1,424 patients with the diagnosis of SpA according to the European Spondyloarthropathy Study Group (ESSG) submitted to a common protocol of investigation and recruited in 29 reference centers participants of the Brazilian Registry of Spondyloarthritis (RBE – Registro Brasileiro de Espondiloartrites). Patients were divided in two groups: age at onset<16 years (JOSpA group) and age at onset ≥ 16 years (AOSpA group). Results Among the 1,424 patients, 235 presented disease onset before 16 years (16.5%). The clinical and epidemiologic variables associated with JOSpA were male gender (p<0.001), lower limb arthritis (p=0.001), enthesitis (p=0.008), anterior uveitis (p=0.041) and positive HLA-B27 (p=0.017), associated with lower scores of disease activity (Bath Ankylosing Spondylitis Disease Activity Index – BASDAI; p=0.007) and functionality (Bath Ankylosing Spondylitis Functional Index – BASFI; p=0.036). Cutaneous psoriasis (p<0.001), inflammatory bowel disease (p=0.023), dactylitis (p=0.024) and nail involvement (p=0.004) were more frequent in patients with adult-onset SpA. Conclusions Patients with JOSpA in this large Brazilian cohort were characterized predominantly by male gender, peripheral involvement (arthritis and enthesitis), positive HLA-B27 and lower disease scores.

https://doi.org/10.1016/j.rbr.2014.06.005 3636 downloads

11.

Production of anti-Cryptosporidium polyclonal antibodies and standardization of direct immunofluorescence for detecting oocysts in water
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Osaki, Silvia Cristina

; Costa, Adriana Oliveira

; Troiano, Ludmilla Della Coletta

; Kruger, Ernesto Renato

; Pereira, Juliana Tracz

; Fernandes, Nelson Luis Mello

; Silva, Márcia Benedita de Oliveira

; Soccol, Vanete Thomaz

Revista da Sociedade Brasileira de Medicina Tropical

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oct 2011, Volume 44 N. 5 Pages 587 - 590

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

INTRODUÇÃO: A produção de anticorpos policlonais anti-Cryptosporidium e sua utilização na imunofluorescência para determinar a presença de Cryptosporidium em água são descritas no presente trabalho. MÉTODOS: Dois coelhos foram imunizados com antígeno solúvel e particulado provenientes de oocistos purificados de Cryptosporidium. O soro produzido foi preparado para a extração de imunoglobulinas G, que foram purificadas e conjugadas com isotiocianato de fluoresceína (FITC). Lâminas contendo quantidades conhecidas de oocistos foram preparadas para determinar a sensibilidade da técnica. Para testar a especificidade foram preparadas lâminas contendo cistos de Giardia duodenalis. RESULTADOS: O conjugado foi usado com sucesso em amostras de água contaminadas experimentalmente com oocistos de Cryptosporidium, sendo capaz de detectar até cinco oocistos/spots que corresponde a uma contaminação de 250 oocistos/mL. CONCLUSÕES: As três imunizações realizadas nos coelhos foram suficientes para produção de anticorpos contra Cryptosporidium; a reação de imunofluorescência direta padronizada permitiu a detecção de cinco oocistos em 20% das amostras; não houve reação cruzada com cistos de Giardia duodenalis.

INTRODUCTION: The production of anti-Cryptosporidium polyclonal antibodies and its use in direct immunofluorescence assays to determine the presence of Cryptosporidium in water are described in the present work. METHODS: Two rabbits were immunized with soluble and particulate antigens from purified Cryptosporidium oocysts. The sera produced were prepared for immunoglobulin G extraction, which were then purified and conjugated with fluorescein isothiocyanate (FITC). Slides containing known amounts of oocysts were prepared to determine the sensitivity of the technique. To test the specificity, slides containing Giardia duodenalis cysts were prepared. RESULTS: The conjugate was successfully used in water samples experimentally contaminated with Cryptosporidium oocysts, and it was possible to detect up to five oocysts/spot, corresponding to contamination of 250 oocysts/mL. CONCLUSIONS: The three immunizations performed in the rabbits were enough to produce antibodies against Cryptosporidium, the standard direct immunofluorescence assay permitted the detection of five oocysts in 20% of the samples, and no cross-reaction with Giardia duodenalis cysts occurred.

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12.

Microsurgical clipping in forty patients with unruptured anterior cerebral circulation aneurysms: an investigation into cognitive outcome
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Pereira-Filho, Arthur A

; Pereira, Adriana G

; Faria, Mário B

; Lima, Leonardo C.S

; Portuguez, Mirna W

; Kraemer, Jorge L

Arquivos de Neuro-Psiquiatria

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oct 2010, Volume 68 N. 5 Pages 770 - 774

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: É consenso que a maioria dos aneurismas intracranianos não-rotos (AINR) podem ser tratados com aceitável taxa de morbidade. Entretanto, alguns estudos reportaram déficits cognitivos no pós-operatório, sugerindo que poderiam ser atribuídos ao dano cirúrgico. O objetivo desse estudo é avaliar a função cognitiva antes e após clipagem microcirúrgica em pacientes com AINR. MÉTODO: Uma série de 40 pacientes com AINR submetidos à clipagem microcirúrgica foi estudada. A avaliação cognitiva (Mini Exame do Estado Mental, MEEM) foi realizada antes e após a intervenção cirúrgica. A análise estatística foi realizada com teste "t" de Student e análise de variância (ANOVA). RESULTADOS: A média dos escores do MEEM na análise pré-operatória foi 28,12 (DP, 1,34). No período pós-operatório, a média dos escores foi 28,40 (DP, 1,46). Não houve diferença estatística (teste "t" de Student; p=0,315). A ANOVA não encontrou associações independentes entre os escores de MEEM e idade, hipertensão, tabagismo, dislipidemia, educação e características dos aneurismas (topografia, número, lado e tamanho). CONCLUSÃO: O presente estudo sugere que a clipagem microcirúrgica não está associada a danos cognitivos maiores em pacientes com AINR

OBJECTIVE: It is a consensus that most unruptured intracranial aneurysms (UIA) can be treated with acceptably low morbidity. However, some studies recently reported postoperative cognitive impairment, suggesting that it could be attributable to surgical damage. Our goal is to evaluate cognitive function before and after microsurgical clipping in patients with UIA. METHOD: A consecutive series of 40 patients who underwent microsurgical clipping for UIA were studied. The cognitive assessment (Mini Mental State Examination, MMSE) was performed immediately before and at least one month after surgery. Paired Student's "t" test and analysis of variance (ANOVA) were used for statistical purposes. RESULTS: The mean MMSE score in the preoperative analysis was 28.12 (SD, 1.34). In the postoperative period the mean MMSE score was 28.40 (SD, 1.46). Paired Student's "t" test was applied to the scores and no significant difference was found (p=0.315). ANOVA did not find independent associations between MMSE scores and age, hypertension, smoking, dyslipidemia, education, aneurysm location, number, laterality or size. CONCLUSION: The present study suggests that microsurgical clipping for UIA does not result in major cognitive dysfunction as determined by the MMSE

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13.

Expolinear model on soybean growth in Argentina and Brazil
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Confalone, Adriana Elisabet

; Bernardes, Marcos Silveira

; Costa, Luiz Claudio

; Righi, Ciro Abbud

; Dourado Neto, Durval

; Martin, Thomas Newton

; Manfron, Paulo Augusto

; Pereira, Carlos Rodrigues

Ciência Rural

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may 2010, Volume 40 N. 5 Pages 1009 - 1016

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Prever o crescimento e a produtividade das culturas com precisão é uma das principais preocupações das ciências agrícolas. Com esse propósito, modelos mecanísticos de crescimento de culturas têm sido desenvolvidos e testados. A adequação do modelo expolinear de crescimento de culturas foi avaliada para prever as modificações de crescimento de cultivares de soja (Glycine max L. Merrill) de crescimento determinado e indeterminado, submetidas a déficit hídrico em diferentes estádios fenológicos. Um experimento foi conduzido em Azul/Argentina e outro em Viçosa/Brasil durante as estações de cultivo (1997/1998, 1998/1999 e 2002/2003). O modelo expolinear foi ajustado aos dados de fitomassa seca obtidos de cada tratamento. O modelo apresentou sensibilidade do parâmetro Rm (taxa máxima de crescimento relativo da cultura - g g-1 day-1) à variação na temperatura do ar; do parâmetro Cm (taxa máxima de crescimento da cultura - g m-2 day-1) à radiação solar; e do parâmetro Tb (perda em tempo - dias) ao estresse hídrico. Os valores de Cm foram maiores sem restrições hídricas, apresentando, em ambos os países, uma correlação direta com a radiação solar. Sem restrições hídricas, os valores de Rm foram menores quando a temperatura média do ar durante o ciclo foi menor. Sob estresse hídrico, a cultura mostrou uma tendência a apresentar valores de Rm maiores. Os valores de Tb foram menores nos tratamentos irrigados e maiores nos tratamentos com deficiência hídrica. A análise dos resultados mostrou claramente a capacidade do modelo expolinear para simular as diferentes taxas de crescimento da cultura da soja como uma consequência das condições climáticas.

Predicting crop growth and yield with precision are one of the main concerns of the agricultural science. For these purpose mechanistic models of crop growth have been developed and tested worldwide. The feasibility of an expolinear model for crop growth was evaluated on predicting growth modification on soybean (Glycine max L. Merrill) of determined and undetermined growth cultivars, submitted to water restrictions imposed on different phenological stages. An experiment was carried out in Azul/Argentina and in Viçosa/Brazil during the growing seasons (1997/1998, 1998/1999 and 2002/2003). The expolinear model was adjusted to the dry-matter data obtained from each treatment. The model showed sensibility of Rm (maximum relative growth rate of the culture - g g-1 day-1) to variation in air temperature; of Cm (maximum growth rate of the culture - g m-2 day-1) to solar radiation and of Tb (lost time -day) to water stress. Cm values were higher without water restriction presenting, in both countries, a direct correlation with solar radiation. Without water restrictions, Rm values were lower when the average air temperature during the cycle was lower. It was observed that under water stress the culture had a bias to present higher Rm values. Tb was lower in the irrigated treatments than in those with water deficits. The analysis of the outputs clearly shows the feasibility of the expolinear model to explain the differential growth rates of soybean as a consequence of climatic conditions.

2829 downloads Cited 3 times in SciELO

14.

Genetic control on the performance of common bean differential cultivars to Colletotrichum lindemuthianum races
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Gonçalves-Vidigal, Maria Celeste

; Bonett, Lucimar Pereira

; Vidigal Filho, Pedro Soares

; Gonela, Adriana

; Ribeiro, Aliny Simony

Brazilian Archives of Biology and Technology

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jul 2007, Volume 50 N. 4 Pages 579 - 586

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Dentre as várias doenças que causam problemas no cultivo de feijão comum (Phaseolus vulgaris L.), uma das mais importantes é a antracnose causada pelo fungo Colletotrichum lindemuthianum (Saac. et Magn.) Scrib., a qual pode resultar em severas perdas na produtividade de grãos. O controle genético, nas cultivares diferenciais Michelite, Michigan Dark Red Kidney, Perry Marrow, Cornell 49-242, PI 207262, AB 136 e G 2333 e seus 21 híbridos dialélicos, foi obtido usando a metodologia de Hayman (1954). Todas as populações F1s foram avaliadas em relação à raça 89, enquanto que os híbridos envolvendo a cultivar G 2333 não foram avaliados com a raça 31. Os resultados demonstraram que os efeitos aditivos foram predominantes para a raça 31, enquanto os efeitos gênicos não aditivos foram mais importantes na reação de incompatibilidade das cultivares a raça 89. Os resultados ainda evidenciaram um elevado coeficiente de determinação genética para a raça 31. Os cultivares G 2333, AB 136 e PI 207262 são os mais indicados para programas de melhoramento visando à obtenção de cultivares resistentes à antracnose por possuírem maior proporção de alelos dominantes para resistência às raças 31 e 89.

The aim of this work was to perform genetic control on differential cultivars Michelite, Michigan Dark Red Kidney, Perry Marrow, Cornell 49-242, PI 207262, AB 136 and G 2333 and its diallel hybrids by using Hayman's methodology. The entire F1s populations were evaluated in relation to race 89, whereas the race 31, the diallel scheme did not include the cultivar G 2333. The results showed that the additive effects were predominant for the 31 race, while non-additive effects were more important for race 89. The results demonstrated a high coefficient of genetic determination for the race 31. The G 2333, AB 136 and PI 207262 cultivars should be recommended obtaining cultivar resistant to anthracnose by backcross programs because theyshowed the highest proportion of resistant dominant alleles for the races 31 and 89.

2007 downloads Cited 2 times in SciELO

15.

Clastogenicity of Piper cubeba (Piperaceae) seed extract in an in vivo mammalian cell system
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Junqueira, Adriana Pereira Freire

; Perazzo, Fabio Ferreira

; Souza, Gustavo Henrique Bianco

; Maistro, Edson Luis

Genetics and Molecular Biology

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2007, Volume 30 N. 3 Pages 656 - 663

Abstract: En | Text: En | PDF: En

The plant Piper cubeba is widely distributed in tropical and subtropical regions and is used medically for various purposes but has not yet been evaluated for genotoxicity. We used male and female Swiss mice and Wistar rats and the comet assay and micronucleus test to investigate the mutagenic potential of a crude extract of P. cubeba seeds. The rodents were administered 0.5 g kg-1, 1.0 g kg-1 and 1.5 g kg-1 of the extract by gavage. For the Swiss mice, peripheral blood was collected 24 h after treatment for the comet assay, and at 48 and 72 h for the micronucleus test. For the Wistar rats, peripheral blood and hepatic cells were collected for the comet assay and bone marrow cells were collected for the micronucleus test 24 h after treatment. At 1.5 g kg-1, the highest dose tested, the extract induced a statistically significant increase in both the mean number of micronucleated polychromatic erythrocytes and the level of DNA damage in the rodent cell types analyzed. Under our experimental conditions, the P. cubeba seed extract was genotoxic in vivo when administered orally to mice and rats.

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