Resumo: A germinação da semente do café (Coffea arabica L.) é resultado de eventos que ocorrem simultaneamente no embrião e endosperma. Para entender os mecanismos moleculares responsáveis por estes eventos, nós realizamos uma análise transcricional do embrião e dos endospermas micropilar e lateral de sementes embebidas por 10 dias. O sequenciamento obteve contigs que codificam 16.813 proteínas. Destas, 14.005 (~ 83%) foram altamente similares a pelo menos uma sequência de proteína na base de dados nr. 162 genes foram significativamente expressos em embrião, 36 em endosperma micropilar e 72 em endosperma lateral. A análise de tecido-especificidade dos genes significativamente expressos mostrou que a maior proporção de genes específicos está no embrião (113/162, ~70%), enquanto 11 genes foram expressos nos endospermas lateral e micropilar. No embrião, os genes foram associados principalmente com estresse abiótico, crescimento celular e comunicação intercelular. Nos endospermas micropilar e lateral, eles foram associados com estresse abiótico e degradação da parede celular. A acurácia da análise de RNA-seq foi confirmada por RT-qPCR. Este trabalho acrescenta novas informações sobre os mecanismos moleculares envolvidos na germinação de sementes de café.

Abstract: Germination of the coffee (Coffea arabica L.) seed is the result of events that occur simultaneously in the embryo and endosperm. To understand the molecular mechanisms responsible for these events, we undertook a transcriptome analysis of embryo, micropylar and lateral endosperms from 10-day-imbibed seeds. The sequencing yielded contigs coding for 16,813 proteins. From those, 14,005 (~ 83%) were highly similar to at least one protein sequence in the nr database. 162 genes were significantly expressed in the embryo, 36 in the micropylar endosperm and 72 in the lateral endosperm. The tissue specificity analysis of the significantly expressed genes showed that the embryo had the highest proportion of specific genes (113/162, ~70%), while 11 were expressed in the micropylar and lateral endosperms. In the embryo, genes were mainly associated with abiotic stress, cell growth, and intercellular communication. In the micropylar and lateral endosperms, they were associated with abiotic stress and cell wall degradation. The accuracy of RNA-seq data was confirmed by RT-qPCR. This work adds new information about the molecular mechanism involved in coffee seed germination.

Resumo O objetivo deste estudo foi avaliar a relação entre a percepção sobre os serviços odontológicos e a saúde bucal numa população de hipertensos e diabéticos adscritos à Estratégia Saúde da Família em Alfenas, MG, Brasil. Estudo domiciliar, descritivo-analítico, com amostra randomizada e estratificada, composta por 186 sujeitos. Aplicaram-se os índices: Dentes Cariados, Perdidos e Obturados (CPOD); Índice de Dentes Funcionais (FS-T); Índice de Cuidados Odontológicos (ICO); Avaliação do uso e necessidade de próteses; Questionário de Avaliação da Saúde Bucal na Atenção Primária (ASBAP-usuário). Observou-se predomínio de mulheres (67,74%), hipertensos não diabéticos (58,60%) e idosos (52,69%) idade média de 64,26 (± 12,22) anos. Registrou-se CPOD = 27,00 (± 6,24); FS-T=8,94 (± 10,28); ICO=19,42 (± 26,80); 39,78% de uso de próteses parciais, 56,45% de próteses totais e necessidades de 36,02% de próteses parciais e 28,49% de próteses totais. No ASBAP-usuário, prevaleceram avaliações positivas dos serviços. A Saúde Bucal mostrou-se melhor entre os sujeitos que relataram bom vínculo com o dentista e pior entre os que consideraram bons os equipamentos utilizados nos Serviços, sendo ainda pontos críticos: acesso, rapidez e comunicação profissional-paciente.

Abstract The aim of this study was to evaluate the relationship between the perception of dental care services and oral health conditions in a hypertensive and diabetic population subscribed to the Family Health Strategy in the city of Alfenas, Minas Gerais, Brazil. This was a domiciliary, descriptive-analytical study with random and stratified sample, consisting of 186 individuals. The following indexes were applied: Decayed, Missing and Filled Teeth (DMFT); Filled and Sound Teeth (FS-T); Dental Care Index (DCI); Evaluation of the use and need of dentures; Oral health for primary care assessment questionnaire (OHPCA). A predominance of women (67.74%), hypertensive non-diabetic people (58.60%) and elderly people (52.69%), with an average age of 64.26 (± 12.22) years, was observed. We noticed a total DMFT of 27.00 (± 6.24); FS-T = 8.94 (± 10.28); DCI = 19.42 (± 26.80); 39.78% of partial dentures usage, 56.45% of total prosthesis usage with necessity of 36.02% of partial dentures and 28.49% of total prosthesis. In the OHPCA questionnaire, positive assessments of services were prevalent. Oral Health proved to be best among those individuals who reported good relationship with their dentists and worse among those individuals who considered as good the equipment used in the services. The critical aspects were: access, speed and professional-patient communication.

Resumo O objetivo deste estudo foi avaliar a qualidade de vida relacionada às condições clínicas de saúde bucal entre hipertensos e diabéticos de Alfenas, MG, Brasil. Estudo domiciliar, descritivo-analítico, transversal, com amostra randomizada, sistemática, estratificada por Equipe Saúde da Família, composta por 218 sujeitos. Aplicou-se os índices CPOD, T-Health, FS-T, SiC index, uso e necessidade de próteses e OHIP-14. A maioria dos sujeitos (56,42%) apresenta apenas Hipertensão Arterial, é do sexo feminino (67,43%), com idade média de 64,83 (± 11,99), variando entre 35 e 93 anos. Não se observou diferenças significativas para as variáveis entre hipertensos, diabéticos e hipertensos-diabéticos. Registrou-se CPOD = 27,16 (± 6,15), com 22,94 (± 10,46) dentes perdidos; T-Health = 5,23 (± 6,52); FS-T = 8,53 (± 10,12) e SiC = 32 (± 0,00). Dos sujeitos, 85,78% usavam próteses (58,72% Prótese Total) e 61,01% necessitavam das mesmas (58,26% no arco inferior). As correlações entre OHIP-14 (5,37 [± 4,95]) e condições clínicas evidenciaram a presença de dentes afetando dimensões psicológicas, além de uso e necessidade de próteses associadas a impactos físicos e sociais (p < 0,05). Concluiu-se que edentulismo, uso e necessidade de próteses afetaram a qualidade de vida de hipertensos e diabéticos em aspectos psicológicos, físicos e sociais.

Abstract This research aimed to assess the oral health related to quality of life among hypertensive and diabetic patients in the city of Alfenas, Brazil. This was a domiciliary-based, descriptive-analytical, cross-sectional research with a random, systematic sample stratified by the Family Health Team, consisting of 218 individuals. The following indexes were applied: DMFT, T-Health, FS-T, SiC index, use and need of prosthesis and OHIP-14. Most of the patients (56.42%) had only high blood pressure, were females (67.43%), with an average age of 64.83 (±11.99) years old, varying between 35 and 93 years old. No significant differences on the variables between hypertensives, diabetics and hypertensive-diabetics were noticed. The following data was registered: DMFT=27.16 (± 6.15), with 22.94 (± 10.46) of missing teeth; T-Health=5.23 (± 6.52); FS-T=8.53 (± 10.12) and SiC=32 (± 0.00). 85.78% of the individuals were using prosthesis (58.72% Dentures) and 61.01% needed prostheses (58.26% in the jaw).The correlations between OHIP-14 (5.37 [± 4.95]) and oral health evidenced the increase in the number of teeth affecting psychological dimensions, besides the use and need of prostheses were associated to physical and social impacts (p < 0.05). We concluded that edentulism, use and need of prostheses affected quality of life in hypertensive and diabetic patients concerning psychological, physical and social aspects.

Resumo O objetivo deste estudo foi avaliar a qualidade de vida relacionada às condições clínicas de saúde bucal entre hipertensos e diabéticos de Alfenas, MG, Brasil. Estudo domiciliar, descritivo-analítico, transversal, com amostra randomizada, sistemática, estratificada por Equipe Saúde da Família, composta por 218 sujeitos. Aplicou-se os índices CPOD, T-Health, FS-T, SiC index, uso e necessidade de próteses e OHIP-14. A maioria dos sujeitos (56,42%) apresenta apenas Hipertensão Arterial, é do sexo feminino (67,43%), com idade média de 64,83 (± 11,99), variando entre 35 e 93 anos. Não se observou diferenças significativas para as variáveis entre hipertensos, diabéticos e hipertensos-diabéticos. Registrou-se CPOD = 27,16 (± 6,15), com 22,94 (± 10,46) dentes perdidos; T-Health = 5,23 (± 6,52); FS-T = 8,53 (± 10,12) e SiC = 32 (± 0,00). Dos sujeitos, 85,78% usavam próteses (58,72% Prótese Total) e 61,01% necessitavam das mesmas (58,26% no arco inferior). As correlações entre OHIP-14 (5,37 [± 4,95]) e condições clínicas evidenciaram a presença de dentes afetando dimensões psicológicas, além de uso e necessidade de próteses associadas a impactos físicos e sociais (p < 0,05). Concluiu-se que edentulismo, uso e necessidade de próteses afetaram a qualidade de vida de hipertensos e diabéticos em aspectos psicológicos, físicos e sociais.

Abstract This research aimed to assess the oral health related to quality of life among hypertensive and diabetic patients in the city of Alfenas, Brazil. This was a domiciliary-based, descriptive-analytical, cross-sectional research with a random, systematic sample stratified by the Family Health Team, consisting of 218 individuals. The following indexes were applied: DMFT, T-Health, FS-T, SiC index, use and need of prosthesis and OHIP-14. Most of the patients (56.42%) had only high blood pressure, were females (67.43%), with an average age of 64.83 (±11.99) years old, varying between 35 and 93 years old. No significant differences on the variables between hypertensives, diabetics and hypertensive-diabetics were noticed. The following data was registered: DMFT=27.16 (± 6.15), with 22.94 (± 10.46) of missing teeth; T-Health=5.23 (± 6.52); FS-T=8.53 (± 10.12) and SiC=32 (± 0.00). 85.78% of the individuals were using prosthesis (58.72% Dentures) and 61.01% needed prostheses (58.26% in the jaw).The correlations between OHIP-14 (5.37 [± 4.95]) and oral health evidenced the increase in the number of teeth affecting psychological dimensions, besides the use and need of prostheses were associated to physical and social impacts (p < 0.05). We concluded that edentulism, use and need of prostheses affected quality of life in hypertensive and diabetic patients concerning psychological, physical and social aspects.

Resumo O objetivo deste estudo foi avaliar a relação entre a percepção sobre os serviços odontológicos e a saúde bucal numa população de hipertensos e diabéticos adscritos à Estratégia Saúde da Família em Alfenas, MG, Brasil. Estudo domiciliar, descritivo-analítico, com amostra randomizada e estratificada, composta por 186 sujeitos. Aplicaram-se os índices: Dentes Cariados, Perdidos e Obturados (CPOD); Índice de Dentes Funcionais (FS-T); Índice de Cuidados Odontológicos (ICO); Avaliação do uso e necessidade de próteses; Questionário de Avaliação da Saúde Bucal na Atenção Primária (ASBAP-usuário). Observou-se predomínio de mulheres (67,74%), hipertensos não diabéticos (58,60%) e idosos (52,69%) idade média de 64,26 (± 12,22) anos. Registrou-se CPOD = 27,00 (± 6,24); FS-T=8,94 (± 10,28); ICO=19,42 (± 26,80); 39,78% de uso de próteses parciais, 56,45% de próteses totais e necessidades de 36,02% de próteses parciais e 28,49% de próteses totais. No ASBAP-usuário, prevaleceram avaliações positivas dos serviços. A Saúde Bucal mostrou-se melhor entre os sujeitos que relataram bom vínculo com o dentista e pior entre os que consideraram bons os equipamentos utilizados nos Serviços, sendo ainda pontos críticos: acesso, rapidez e comunicação profissional-paciente.

Abstract The aim of this study was to evaluate the relationship between the perception of dental care services and oral health conditions in a hypertensive and diabetic population subscribed to the Family Health Strategy in the city of Alfenas, Minas Gerais, Brazil. This was a domiciliary, descriptive-analytical study with random and stratified sample, consisting of 186 individuals. The following indexes were applied: Decayed, Missing and Filled Teeth (DMFT); Filled and Sound Teeth (FS-T); Dental Care Index (DCI); Evaluation of the use and need of dentures; Oral health for primary care assessment questionnaire (OHPCA). A predominance of women (67.74%), hypertensive non-diabetic people (58.60%) and elderly people (52.69%), with an average age of 64.26 (± 12.22) years, was observed. We noticed a total DMFT of 27.00 (± 6.24); FS-T = 8.94 (± 10.28); DCI = 19.42 (± 26.80); 39.78% of partial dentures usage, 56.45% of total prosthesis usage with necessity of 36.02% of partial dentures and 28.49% of total prosthesis. In the OHPCA questionnaire, positive assessments of services were prevalent. Oral Health proved to be best among those individuals who reported good relationship with their dentists and worse among those individuals who considered as good the equipment used in the services. The critical aspects were: access, speed and professional-patient communication.

The objective of this work was to assess the functionality of the glycolytic pathways in the bacterium Xylella fastidiosa. To this effect, the enzymes phosphoglucose isomerase, aldolase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase of the glycolytic pathway, and glucose 6-phosphate dehydrogenase of the Entner-Doudoroff pathway were studied, followed by cloning and expression studies of the enolase gene and determination of its activity. These studies showed that X. fastidiosa does not use the glycolytic pathway to metabolize carbohydrates, which explains the increased duplication time of this phytopatogen. Recombinant enolase was expressed as inclusion bodies and solubilized with urea (most efficient extractor), Triton X-100, and TCA. Enolase extracted from X. fastidiosa and from chicken muscle and liver is irreversibly inactivated by urea. The purification of enolase was partial and resulted in a low yield. No enzymatic activity was detected for either recombinant and native enolases, aldolase, and glyceraldehyde-3-phosphate dehydrogenase, suggesting that X. fastidiosa uses the Entner-Doudoroff pathway to produce pyruvate. Evidence is presented supporting the idea that the regulation of genes and the presence of isoforms with regulation patterns might make it difficult to understand the metabolism of carbohydrates in X. fastidiosa.