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Abstract The iAtlantic Project has established an international collaborative strategy to improve mapping and characterization of deep and open ocean ecosystems in understudied regions of the Atlantic and evaluate their health. In December 2022, the first iAtlantic expedition in the South Atlantic set off to map and explore seafloor ecosystems in the Santos Basin slope (200-1,000 m depths) in collaboration with the Petrobras ‘Santos Basin - Regional Characterization Project.’ The 17-day ‘iAtlantic_BR10-Petrobras’ cruise was conducted on board the research vessel NPqHOc Vital de Oliveira (Brazilian Navy) and performed (a) water column structure characterization, (b) seafloor morphology mapping, (c) description of benthic habitats and communities by seafloor imagery and biological/ geological sampling, and (d) ex-situ experiments to assess the functioning of sedimentary ecosystems and their responses to climate-related environmental changes. This study describes the rationale behind the iAtlantic_BR10-Petrobras cruise science plan, reports its sampling strategy and methods, and summarizes its collected data and preliminary results.

https://doi.org/10.1590/2675-2824071.23069jaap

Morfoanatomía comparada del vástago adulto de Conyza bonariensis var. bonariensis y C. bonariensis var. angustifolia (Asteraceae)
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Perez, Beatriz

; Apóstolo, Nancy M.

Bonplandia

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jun 2022, Volume 31 N. 1 Pages 27 - 46

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen: Conyza bonariensis, una especie medicinal e importante maleza nativa de Argentina, está representada por las variedadesC. bonariensisvar.bonariensisyC. bonariensisvar.angustifolia. El objetivo del trabajo es comparar morfológica y anatómicamente los vástagos de ambas variedades en su etapa adulta, con el fin de contribuir a su identificación. Se analizaron ejemplares en estado vegetativo y reproductivo recolectados de poblaciones naturales. Los vástagos fueron divididos en tres zonas, superior, media e inferior, para caracterizar su morfoanatomía por medio de técnicas convencionales para microscopía óptica y electrónica de barrido. Entre las características halladas con valor diagnóstico se describen el tamaño, forma y margen de las hojas, tipo de indumento, densidad de tricomas y de estomas, forma de las células del clorénquima en los tallos y tipo de mesófilo. Muchas de estas características no fueron constantes entre las distintas zonas del vástago analizado, particularmente en el vástago vegetativo. Estos resultados indican que existen caracteres morfoanatómicos cuali- y cuantitativos que permiten la identificación taxonómica deC. bonariensisa nivel infraespecífico, los cuales, a su vez, pueden variar según el tipo y zona del vástago adulto analizado.

Summary: Conyza bonariensis, a medicinal species and important weed native to Argentina, is represented by the varietiesC. bonariensisvar.bonariensisandC. bonariensisvar.angustifolia. The aim of this work is to compare the morphology and anatomy of the shoots of both varieties in their adult phase in order to contribute to their identification. Specimens in the vegetative and reproductive state collected from natural populations were analyzed. The shoots were divided into three zones, upper, middle and lower, to characterize their morphoanatomy using conventional techniques for light and scanning electron microscopy. Among the characteristics found to have diagnostic value are the size, shape and margin of the leaves, type of indumentum, density of trichomes and stomata, type of mesophyll and shape of the chlorenchyma cells in the stems. Many of these characteristics were not constant among the different zones of the shoot, particularly in the vegetative shoots. These results indicate that there are qualitative and quantitative morphoanatomical characters that allow the taxonomic identification ofC. bonariensisat the infraspecific level, which in turn may vary depending on the type and zone of the adult shoot analyzed.

https://doi.org/10.30972/bon.3115805 26 downloads

Perinatal exposure to a high-fat diet alters proopiomelanocortin, neuropeptide Y and dopaminergic receptors gene expression and the food preference in offspring adult rats
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Santos, L. S.

; Matos, R. J. B.

; Cordeiro, G. S.

; Perez, G. S.

; Santo, D. A. E.

; Silva, R. T.

; Gonçalves, M. S.

; Boaventura, G. T.

; Deiró, T. C. B. J.

; Barreto-Medeiros, J. M.

Brazilian Journal of Biology

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2022, Volume 82 elocation e234855

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo A exposição à dieta hiperlipídica pode alterar o controle da ingestão de alimentos, promovendo hiperfagia e obesidade. O objetivo deste estudo foi investigar os efeitos dessa dieta sobre a expressão gênica dos receptores de dopamina (drd1 e drd2), da proopiomelanocortina (pomc) e neuropeptídeo Y (npy), e preferência alimentar em ratos adultos. Ratas Wistar foram alimentadas com uma dieta hiperlipídica ou controle durante a gestação e lactação. Os descendentes foram alocados em grupos: Lactação – Controle (C) e Hiperlipídica (H). Pós-desmame – Controle Controle (CC), descendentes das genitoras do grupo controle e alimentados com dieta controle após o desmame; Controle Hiperlipídica (CH), descendentes das genitoras do grupo controle e alimentados com dieta hiperlipídica após o desmame; Hiperlipídica Controle (HC), descendentes das genitoras do grupo hiperlipídica e alimentados com dieta controle após o desmame; Hiperlipídica Hiperlipídica (HH), descendentes das genitoras do grupo hiperlipídica e alimentados com dieta hiperlipídica após o desmame. Os grupos CH e HH apresentaram maior expressão de drd1 em comparação ao CC. O drd2 de CH e HC apresentou maior expressão gênica que o CC. HH apresentou maior expressão de pomc em comparação com os outros grupos. O HC também apresentou maior expressão de pomc em comparação ao CH. O npy do HH apresentou maior expressão em relação ao CH e HC. HH e HC tiveram uma preferência maior por uma dieta rica em gordura no 102º dia de vida. A dieta hiperlipídica alterou a expressão gênica dos drd1, drd2, pomc e npy e influenciou na preferência alimentar pela dieta hiperlipídica.

Abstract Exposure to the hight-fat diet may alter the control of food intake promoting hyperphagia and obesity. The objective of this study was to investigate the effects of this diet on dopamine receptors (drd1 and drd2), proopiomelanocortin (pomc), neuropeptideY (npy) genes expression, and preference food in adult rats. Wistar female rats were fed a hight-fat or control diet during pregnancy and lactation. The offspring were allocated into groups: Lactation – Control (C) and High-fat (H). Post-weaning – Control Control (CC), offspring of mothers C, fed a control diet after weaning; Control Hight-fat (CH), offspring of mothers C, fed a hight-fat diet after weaning; Hight-fat Control (HC), offspring of mothers H, fed with control diet after weaning; and Hight-fat Hight-fat (HH), offspring of mothers H, fed a H diet after weaning. The groups CH and HH presented greater expression of drd1 in comparison to the CC. The drd2 of CH and HC presented higher gene expression than did CC. HH presented higher pomc expression in comparison to the other groups. HC also presented greater expression in comparison to CH. The npy of HH presented greater expression in relation to CH and HC. HH and HC have had a higher preference for a high-fat diet at 102º life’s day. The high-fat diet altered the gene expression of the drd1, drd2, pomc and npy, and influencing the food preference for high-fat diet.

https://doi.org/10.1590/1519-6984.234855 220 downloads

Murinometric measurements and retroperitoneal adipose tissue in young rats exposed to the high-fat diet: Is there correlation?
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Macêdo, A. P. A

; Cordeiro, G. S.

; Santos, L. S.

; Santo, D. A. E.

; Perez, G. S.

; Couto, R. D.

; Machado, M. E. P. C.

; Medeiros, J. M. Barreto

Brazilian Journal of Biology

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may 2021, Volume 81 N. 2 Pages 246 - 250

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Objetivo Este estudo teve como objetivo verificar a correlação entre medidas murinométricas e tecido adiposo retroperitoneal em ratos expostos à dieta hiperlipídica. Material e métodos: Foram utilizados ratos Wistar machos adultos, descendentes de mães que consumiram dieta hiperlipídica durante a gestação e lactação e alimentados com a mesma dieta após o desmame. Aos 60 dias de vida, foram medidos o peso corporal, o eixo longitudinal e a circunferência da cintura (CC). O Índice de Massa Corporal (IMC) e o Índice de Lee foram calculados para posterior análise da correlação com a quantidade de tecido adiposo retroperitoneal dissecado no mesmo dia. Para análise dos dados, utilizou-se o teste de correlação de Pearson, considerando significância estatística para p <0.05. Resultados: O peso corporal apresentou uma correlação fraca (r= 0,31; p= 0,38) com o tecido adiposo retroperitoneal. Enquanto o longitudinal correlacionou moderadamente e negativo (r= -0,40; p= 0,25). A circunferência abdominal (r = 0,62; p = 0,05), índice de massa corporal (r= 0,61; p= 0,03) e Lee (r=0,69; p= 0,03) correlacionaram-se moderada e positivamente com o tecido adiposo. Conclusão: Entre as medidas murinométricas, o peso e o eixo longitudinal não foram bons indicadores para representar o acúmulo de tecido adiposo retroperitoneal em ratos. No entanto, o índice de Lee parece ser o melhor indicador murinométrico para diagnosticar o acúmulo de gordura retroperitoneal. O IMC, índice de Lee e CA foram parâmetros murinométricos com maior correlação.

Abstract Aim This study aimed to verify the correlation between murine measurements and retroperitoneal adipose tissue in rats exposed to the high-fat diet. Material and methods: Wistar male adult rats, descendants of mothers who consumed a high-fat diet during pregnancy and lactation and fed the same diet after weaning were used. At 60 days of life, body weight, longitudinal axis and waist circumference (WC) were measured. The Body Mass Index (BMI) and the Lee Index were calculated for a posterior analysis of the correlation with the amount of retroperitoneal adipose tissue dissected on the same day. For analysis of the data, the Pearson correlation test was used, considering statistical significance for p <0.05. Results: Body weight had a weak correlation (r= 0.31; p= 0.38) with retroperitoneal adipose tissue. While the longitudinal correlated moderately and negative (r= -0.40; p= 0.25). Abdominal circumference (r= 0.62; p= 0.05), body mass index (r= 0.61; p= 0.03) and Lee (r= 0.69; p= 0.03) correlated moderately and positively with adipose tissue. Conclusion: Among the measured murine measurements, weight and longitudinal axis were not good indicators to represent accumulation of retroperitoneal adipose tissue in rats. However, Lee's index seems to be the best murine marker to diagnose the accumulation of retroperitoneal fat. BMI, CA and Lee index were murine parameters with higher correlation.

https://doi.org/10.1590/1519-6984.221405 547 downloads

Comparative cytogenetic survey of the giant bonytongue Arapaima fish (Osteoglossiformes: Arapaimidae), across different Amazonian and Tocantins/Araguaia River basins
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Oliveira, Ezequiel A. de

; Sassi, Francisco de M. C.

; Perez, Manolo F.

; Bertollo, Luiz A. C.

; Ráb, Petr

; Ezaz, Tariq

; Hatanaka, Terumi

; Viana, Patrik F.

; Feldberg, Eliana

; Oliveira, Edivaldo H. C. de

; Cioffi, Marcelo de B.

Neotropical Ichthyology

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2020, Volume 18 N. 4 elocation e200055

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Os peixes gigantes da América do Sul do gêneroArapaima, comumente conhecidos como pirarucus, são um dos mais icônicos de Osteoglossiformes. Estudos citogenéticos prévios identificaram suas características cariotípicas, entretanto a caracterização da diferenciação citotaxonômica através de suas distribuições geográficas ainda são desconhecidas. Nesse estudo, nós comparamos características cromossômicas utilizando técnicas de citogenética clássica e molecular em populações das bacias dos rios Amazonas e Tocantins-Araguaia, a fim de verificar se há alguma diferenciação entre representantes desse gênero. Nossos dados revelaram que indivíduos de todas as populações apresentam número diploide de 2n=56 cromossomos e que seus cariótipos são compostos de 14 pares de cromossomos meta- e submetacêntricos e 14 pares de subtelo- e acrocêntricos. Os sítios maiores e menores de rDNA estão localizados em pares cromossômicos separados, onde os sítios maiores de rDNA correspondem a grandes blocos heterocromáticos. Hibridizações genômicas comparativas (CGH) mostraram que o genoma dos espécimes dessas populações é amplamente compartilhado, devido à falta de sinais substanciais específicos. Nossos dados de citogenética comparativa do pirarucu sugerem que embora diferenças genéticas significativas ocorram entre populações, os padrões cariotípicos gerais se mantêm conservados.

Abstract The South American giant fishes of the genus Arapaima, commonly known as pirarucu, are one of the most iconic among Osteoglossiformes. Previously cytogenetic studies have identified their karyotype characteristics; however, characterization of cytotaxonomic differentiation across their distribution range remains unknown. In this study, we compared chromosomal characteristics using conventional and molecular cytogenetic protocols in pirarucu populations from the Amazon and Tocantins-Araguaia river basins to verify if there is differentiation among representatives of this genus. Our data revealed that individuals from all populations present the same diploid chromosome number 2n=56 and karyotype composed of 14 pairs of meta- to submetacentric and 14 pairs of subtelo- to acrocentric chromosomes. The minor and major rDNA sites are in separate chromosomal pairs, in which major rDNA sites corresponds to large heterochromatic blocks. Comparative genomic hybridizations (CGH) showed that the genome of these populations shared a great portion of repetitive elements, due to a lack of substantial specific signals. Our comparative cytogenetic data analysis of pirarucu suggested that, although significant genetic differences occur among populations, their general karyotype patterns remain conserved.

https://doi.org/10.1590/1982-0224-2020-0055 1170 downloads

Influence of mother nutrition during pregnancy and/or lactation on offspring food preference in experimental models
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Santos, L. S.

; Cordeiro, G. S.

; Perez, G. S.

; Santo, D. A. E.

; Macêdo, A. P. A.

; Lima, M. S.

; Carneiro, I. B. C.

; Machado, M. E. P. C.

; Deiró, T. C.B. J.

; Barreto-Medeiros, J. M.

Brazilian Journal of Biology

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apr 2019, Volume 79 N. 2 Pages 220 - 232

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Introdução O entendimento das associações entre as preferências alimentares e nutrição materna durante a gravidez e lactação poderia colaborar para a compreensão dos mecanismos da obesidade e fornecer informações para prevenir essa infermidade. Objetivo: Identificar estudos que investigaram os efeitos das intervenções nutricionais durante a gravidez e lactação em preferências alimentares dos descendentes. Método: A revisão foi conduzida com busca de artigos nas bases de dados: Scopus, Pubmed, Medline, Lilacs, Scielo e Science Direct. Os critérios de exclusão utilizados: revisões, estudos em humanos, estudos com drogas ou outras substâncias não-alimentares. Resultados: No final da pesquisa nas bases de dados, 176 referências foram encontradas. Depois de usar os critérios de exclusão, lendo os títulos, resumos e artigos completos, 11 artigos foram selecionados para compor a revisão. Conclusão: Os estudos selecionados sugeriram que a nutrição desequilibrada no início da vida altera a preferência alimentar e componentes neurais relacionadas com o consumo de alimentos gordurosos e açucarados em prole de roedores.

Abstract Introduction Understanding associations between food preferences and maternal nutrition during pregnancy and lactation could inform efforts to understanding the obesity mechanisms and provide insight to prevent it. Objective: To identify studies that investigated the effects of nutritional interventions during the pregnancy and lactation on the food preferences of offspring. Method: The review was conducted with search for articles in the databases: Scopus, Pubmed, Medline, LILACS, Scielo and Science Direct. Exclusion criteria were used: reviews, human studies, studies with drugs or other substances not related to food. Results: At the end of the search in the databases, 176 references were found. After use the exclusion criteria, reading the titles, abstracts and full articles, were selected 11 articles to compose the review. Conclusion: The selected studies suggested that unbalanced nutrition in early life alters the food preference and neural components related to the consumption of fatty and sugary foods in offspring rodents.

https://doi.org/10.1590/1519-6984.179134 1591 downloads

Sexto reporte de eventos adversos con tratamientos biológicos en Argentina: Informe del registro BIOBADASAR
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Gómez, G.

; Pons-Estel, G.

; Citera, G.

; Soriano, E.

; Saurit, V.

; Benavidez, F.

; Velozo, E.

; Dubinsky, D.

; Exeni, I.

; Gobi, C.

; Gómez, G.

; Díaz, M.

; Granel, A.

; Smichowsky, A.

; Garate, G.

; Quinteros, A.

; Cavillon, E.

; Petkovic, I.

; Larroude, M.S.

; de la Sota, M.

; Cappuccio, A.M.

; Berbotto, G.

; Alvarez, A.

; Papasidero, S.

; Lazaro, A.

; Garcia, M.

; Sacnun, M.

; Soares de Souza, S.

; Bertoli, A.

; Bejarano, V.

; Rillo, O.

; Kerzberg, E.

; Quintana, R.

; Agüero, S.

; Somma, L.

; Cruzat, V.

; Battaglitti, C.

; Perez Dávila, A.

; Gallardo, M.

; Pereira, D.

; Verando, M.

; Paira, S.

; Martinez, L.

; Aste, N.

; Pisoni, C.

; Catay, E.

; Graf, C.

; Casado, G.

; de la Vega, M.

Revista argentina de reumatología

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mar 2019, Volume 30 N. 1 Pages 4 - 9

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Objective: Update the results of the BIOBADASAR registry on safety, duration and causes of treatment interruption after 8 years of follow-up. Methods: BIOBADASAR is a safety record of biological therapies established by the Argentine Society of Rheumatology. The description of BIOBADASAR 3.0 is presented, a cohort of 53 centers in Argentina followed prospectively from August 2010 to January 2018. Results: 4656 patients were registered, 6234 treatments [3765 cases (therapy with biologicals) and 2469 controls (non-biological therapy)]. Treatment was interrupted in 44.6% in cases vs. 27.9% in controls. Main cause of discontinuation was due to inefficiency (40% cases vs. 32% controls). There were 3154 adverse events (2230 in cases vs. 924 in controls), of which 13.6% were tombs (9.8% in cases and 3.7% in controls). The most frequent adverse event (AE) in both groups were infections (43.56% in cases vs. 34.31% in controls, RR: 3.42, 95% CI: 3.02-3.88), and the upper airway pathways (14.5%). Neoplasms were published in 78 cases versus 45 controls (RR: 1.98, 95% CI: 1.37-2.86). Conclusions: In this article, there are no different trends regarding safety, duration and causes of interruption of treatment compared to previous reports. Infections were the main causes of treatment discontinuation. The advent of new biological agents and the need for control over long-term security, strengthens the use of this type of registration.

Objetivo: Actualizar los resultados del registro BIOBADASAR sobre seguridad, duración y causas de interrupción del tratamiento luego de 8 años de seguimiento. Métodos: BIOBADASAR es un registro de seguridad de terapias biológicas establecido por la Sociedad Argentina de Reumatología. Se presenta la descripción de BIOBADASAR 3.0, una cohorte compuesta por 53 centros de Argentina seguidos prospectivamente desde agosto de 2010 hasta enero de 2018. Resultados: Se registraron 4656 pacientes, 6234 tratamientos [3765 casos (terapia con biológicos) y 2469 controles (terapia no biológicos)]. Se interrumpió el tratamiento en el 44,6% en los casos vs. 27,9% en los controles. Causa principal de discontinuación fue por ineficacia (40% casos vs. 32% controles). Se presentaron 3154 eventos adversos (2230 en casos vs. 924 en controles), de los cuales el 13,6% fueron graves (9,8% en casos y 3,7% en controles). El evento adverso (EA) más frecuente en ambos grupos fueron las infecciones (43,56% en casos vs. 34,31% en los controles, RR: 3,42; IC 95%: 3,02-3,88), y de ellas las de vías aéreas superiores (14,5%). Las neoplasias se presentaron en 78 casos vs. 45 en controles (RR: 1,98; IC 95%: 1,37-2,86). Conclusiones: En este sexto reporte no se observan tendencias diferentes sobre seguridad, duración y causas de interrupción del tratamiento respecto a informes previos. Las infecciones fueron el principal EA y la ineficacia, seguido por EA y la pérdida de pacientes las principales causas de suspensión del tratamiento. El advenimiento de nuevos agentes biológicos y la necesidad de control en seguridad a largo plazo, fortalece el uso de este tipo de registro.

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An easy and computable approximation for Troesch’s problem by using the Laplace Transform-Homotopy Perturbation Method
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Filobello-Nino, U.

; Vazquez-Leal, H.

; Herrera-May, A. L.

; Jimenez-Fernandez, V. M.

; Cervantes-Perez, J.

; Pereyra-Diaz, D.

; Hoyos-Reyes, C.

; Sandoval-Hernandez, M. A.

; Huerta-Chua, J.

Acta universitaria

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2019, Volume 29 elocation e2065

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract This work introduces the Laplace Transform-Homotopy Perturbation Method (LT-HPM) in order to provide an approximate solution for Troesch’s problem. After comparing figures between exact and approximate solutions, as well as the average absolute relative error (AARE) of the approximate solutions of this research, with others reported in the literature, it can be said that the proposed solutions are accurate and handy. In conclusion, LT-HPM is a potentially useful tool.

Resumen Este artículo propone el método de perturbación homotópica con Transformada de Laplace para encontrar una solución aproximada al problema de Troesch. Después de comparar las figuras entre la solución aproximada y la exacta, así como el error relativo absoluto promedio (AARE) de las soluciones aproximadas en este estudio, con otras reportadas en la literatura, se puede decir que la solución propuesta es, además de práctica, precisa. Se concluye que el método LTHPM es potencialmente útil.

https://doi.org/10.15174/au.2019.2065 364 downloads

Effects of maternal high fat intake during pregnancy and lactation on total cholesterol and adipose tissue in neonatal rats
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Lima, M. S.

; Perez, G. S.

; Morais, G. L.

; Santos, L. S.

; Cordeiro, G. S.

; Couto, R. D.

; Deiró, T. C. B. J.

; Leandro, C. G.

; Barreto-Medeiros, J. M.

Brazilian Journal of Biology

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nov 2018, Volume 78 N. 4 Pages 615 - 618

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Objetivo A obesidade durante a gestação é um dos fatores de risco mais estabelecidos para uma programação negativa em longo prazo. O objetivo do presente estudo foi investigar os efeitos do consumo materno de uma dieta hiperlipídica durante a gestação e lactação no aumento do peso, do tecido adiposo visceral e colesterolemia em ratos neonatos. Métodos Ratos Wistar foram divididos em dois grupos de acordo com a dieta da mãe durante a gestação e lactação: grupo controle (GC, n=12) composto por filhotes de ratas alimentadas com uma dieta padrão (lipídios 4%) e o grupo teste (GT, n=12) composto de filhotes de ratas alimentadas com dieta hiperlipídica (lipídios 23%). O peso dos animais foi aferido em dias alternados até o 22° dia de vida, quando foi coletado sangue para análises bioquímicas. Para a análise estatística utilizou-se os seguintes testes: two way ANOVA, teste de Sidak e teste t de Student, com p< 0,05. Resultados O grupo teste mostrou diferença no ganho de peso, no tecido adiposo visceral e nos níveis de colesterol. Conclusão Uma exposição materna a uma dieta hiperlipídica durante a gestação e lactação pode promover maior ganho ponderal, hipercolesterolemia e um aumento do tecido adiposo em ratos neonatos.

Abstract Aim Obesity during pregnancy is one of the most established risk factors for negative long-term programming. The aim of the present study was to investigate the effects of maternal consumption of a high-fat diet during pregnancy and lactation on the weight gain, visceral adipose tissue and cholesterolemia in neonatal rats. Methods Wistar rats were divided into two groups according to the mother's diet during pregnancy and lactation: Control group (CG, n = 12) were the offspring of rats fed a standard diet (4% lipid) and the Test group (TG, n = 12) were pups rats fed on a high fat diet (23% lipid). The weight of the animals was measured on alternate days until the 22nd day of life, when collected visceral adipose tissue and blood were collected for biochemical analysis. For statistical analysis the Student t test, Sidak´s teste and two way ANOVA was used, with p <0.05. Results the test group showed differences in weight gain, visceral adipose tissue and higher cholesterol. Conclusion a maternal exposure to a high-fat diet during pregnancy and lactation can promote changes in weight gain, hypercholesterolemia and an increase in adipose tissue in neonatal rats.

https://doi.org/10.1590/1519-6984.166788 2146 downloads

10.

Synthesis, Characterization and Evaluation of in vitro Antitumor Activities of Novel Chalcone-Quinolinone Hybrid Compounds
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d’Oliveira, Giulio D. C.

; Moura, Andrea F.

; Moraes, Manoel O. de

; Perez, Caridad N.

; Lião, Luciano M.

Journal of the Brazilian Chemical Society

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nov 2018, Volume 29 N. 11 Pages 2308 - 2325

Abstract: En | Text: En | PDF: En

Chalcone-quinolinone hybrid compounds, as well as the synthesis of such compounds, have few reports in the literature. Such compounds may be quite useful in therapeutics, since various biological activities are reported for both chalcones and quinolinones. In the present work, several novel chalcone-quinolinone compounds have been synthesized. The reaction conditions developed allowed to obtain the compounds in a single step of synthesis from the chalcones. The products precipitated pure and were isolated by filtration. The yields of such reactions, from 45 to 94%, were promising. The product structures were confirmed by nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESI-MS) techniques. Their antitumor activities were evaluated in HCT-116 (colon) cell lines by the 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) test. The half maximal inhibitory concentration (IC50) values obtained for the most active chalcones were between 2.9 and 7.5 and for active quinolinone was 19.3 mg L-1. The antitumor activities results suggest that the class of compounds studied has potential for use in cancer research.

https://doi.org/10.21577/0103-5053.20180108 766 downloads

11.

Physicochemical and mechanical study of Mayan Archeological stony constructive materials
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Ley-Paredes, V. J.

; Cervantes-Uc, J. M.

; Perez-Pacheco, E.

; Ríos-Soberanis, C. R.

; Wakayama, S.

Journal of applied research and technology

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2018, Volume 16 N. 4 Pages 287 - 298

Abstract: En | Text: En | PDF: En

Abstract Mayan civilization monumental architecture has been a vital source of information to gain acknowledgement about its way of life, science and political hierarchy; consequently, examination of constructive materials of Mayan structures by using materials science characterization techniques are noteworthy. This work focuses on the study and characterization of archeological stony materials taken from the main structure on Witzinah archeological site located south of Yucatan, Mexico, in order to determine its constituted elements and chemical components. Such materials were observed under scanning electron microscopy (SEM) to establish, in accord to its micro-structural patterns, the porosity and morphology, and energy-dispersive X-ray spectroscopy (EDX) to determine the chemical compounds. Calorimetric analysis through TGA was carried out in order to identify the presence of organic elements as well as Infrared analysis (FTIR) and nuclear magnetic resonance (1H NMR) to detect polymeric components. Preliminary results indicated that mortars contain saturated fats (esters) that were possibly originated by degradation of natural polymer (elastomeric rubber) known as polyisoprene as well as nano-clay material named Palygorskita (typified in Maya as Sak lu’um), and that both had a direct influence on the properties of the evaluated mortars used for the construction of the site Principal Building.

460 downloads

12.

Effect of annealing atmosphere on optic-electric properties of ZnO thin films
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Bueno, C.

; Pacio, M.

; Osorio, E.

; Perez, R.

; Juarez, H.

Revista mexicana de física

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dec 2017, Volume 63 N. 6 Pages 569 - 574

Abstract: En | Text: En | PDF: En

Abstract In this work the study of the structural, morphologic characteristics, optical and electrical properties of the thin films of ZnO in temperatures and annealing atmospheres different was realized. The films were obtained by the sol-gel method, utilizing zinc acetate dihydrate as the precursor, monoethanolamine (MEA) as a stabilizing agent and 2-methoxyethanol as a solvent and deposited by spin-coating. The films were crystallized at 600, 800 and 1000 ∘ C in oxygen and nitrogen atmospheres. The results obtained by XRD, SEM, photoluminescence and Hall effects of the ZnO films were related and depend strongly on the temperature and atmosphere annealing.

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13.

Resistance training and hormone replacement increase MMP-2 activity, quality and quantity of bone in ovariectomized rats
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Souza, Markus V.C

; Lino, Anderson D. S

; Ruffoni, Leandro G. D

; Domingos, Mateus M.

; Barbosa, Marina R.

; Rodrigues, Maria F C.

; Ferreira, Fabiano C.

; Tomaz, Luciane M.

; Canevazzi, Gustavo H. R.

; Silva, Natália S.

; Teixeira, Jeferson A. A.

; Leite, Richard D.

; Shiguemoto, Gilberto E.

; Perez, Sérgio E. A.

Motriz: Revista de Educação Física

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2017, Volume 23 N. 4 elocation e1017107

Abstract: En | Text: En | PDF: En

Abstract AIMS The aim of the present study was to investigate the influence of resistance training (RT) and hormone replacement (HR) on MMP-2 activity, biomechanical and physical properties bone of ovariectomized (OVX) rats. METHODS Sprague-Dawley female rats were grouped into six experimental groups (n = 11 per group): sham-operated sedentary (SHAM Sed), ovariectomized sedentary (OVX Sed), sham-operated resistance training (SHAM RT), ovariectomized resistance training (OVX RT), ovariectomized sedentary hormone replacement (OVX Sed-HR), and ovariectomized resistance training hormone replacement (OVX RT-HR). HR groups received implanted silastic capsules with a 5% solution of 17β-estradiol (50 mg 17β-estradiol/ml of sunflower oil). In a 12-week RT period (27 sessions; 4-9 climbs) the animals climbed a 1.1 m vertical ladder with weights attached to their tails. Biomechanical and physical bone analyses were performed using a universal testing machine, and MMP-2 activity analysis was done by zymography. RESULTS Bone density and bone mineral content was higher in the RT and HR groups. The MMP-2 activity was higher in the RT and HR groups. The biomechanical analysis (stiffness, fracture load and maximum load) demonstrated better bone tissue quality in the RT associated with HR. CONCLUSION The RT alone as well as when it is associated with HR was efficient in increasing MMP-2 activity, biomechanical and biophysical properties bone of ovariectomized rats.

https://doi.org/10.1590/s1980-6574201700040003 751 downloads

14.

Pruebas de envejecimiento acelerado para determinar vigor de semillas de Prosopis alba de tres procedencias geográficas
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FONTANA, ANA, M. L

; PEREZ, V. R

; LUNA, C. V

Fave. Sección ciencias agrarias

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jul 2016, Volume 15 N. 1

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

In order to create conceptual basis for determining the quality of Prosopis alba´s seeds through accelerated ageing tests, vigor was determinated conducting trials with moist heat (MHAA), dry heat (DHAA) and saline solution (AASS) on seeds from three geographical origins: Santiago del Estero, Formosa and north of Salta province. Significance was determined for Provenance*Treatment interaction and was determined that the MHAA is the only procedure applicable to all provenances because significantly affect the aging index (AI). According to IE measured after MHAA test, Chaco provenance showed the best performance followed by Santiago del Estero and Salta Norte stands, which are equal to each other. Vigor evaluation allowed the provenances to be distinguished through qualities not detected in normal germination test under standard conditions

Con el objeto de crear bases conceptuales para determinar la calidad de semillas de Prosopis alba a través de pruebas de envejecimiento acelerado se determinó el vigor realizando ensayos con calor húmedo (EACH), calor seco (EACS) y solución salina (EASS) en semillas de tres procedencias geográficas: Santiago del Estero, Formosa y Norte de la provincia de Salta. Se demostró significancia para la interacción Procedencia*Tratamiento y se determinó que el EACH al afectar significativamente al índice de envejecimiento es el único procedimiento aplicable a todas las procedencias. Clasificando a cada procedencia según su vigor medido a través del IE luego de la prueba de EACH: el rodal Chaqueño resulta superior a los rodales Santiagueño y Salta Norte, que son similares entre sí. La evaluación de vigor permitió distinguir las procedencias mediante cualidades no detectadas en pruebas de germinación normal en condiciones estándar

1138 downloads

15.

Influencia de la procedencia geográfica sobre los parámetros morfométricos de semillas de Prosopis alba
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Fontana, M. L.

; Perez, V. R.

; Luna, C. V.

Multequina

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jun 2015, Volume 24 N. 1 Pages 33 - 45

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

In order to experimentally evaluate the influence of the geographical origin on the morphometric characteristics of seeds of Prosopis alba, the length, width, thickness, length-to-thickness ratio, volume, shape, color, weight and volumetric weight of seeds were studied on three different populations: Santiago del Estero at river Dulce´s shore, Formosa at Bermejo´s shore, and north of Salta province. The results showed significant differences in length, width and weight of seeds from different provenances. Among them, the weight of 1000 seeds presents the highest coefficient of variation, indicating that is the parameter with greater differences among the analyzed populations.

Con el objeto de evaluar experimentalmente la influencia de la procedencia geográfica sobre las características morfométricas de simientes de Prosopis alba, se estudiaron las variables longitud, ancho, espesor, relación longitud-espesor, volumen, forma, color, peso y peso volumétrico de semillas provenientes de tres poblaciones distintas: Santiago del Estero a orillas del río Dulce, Formosa a orillas del río Bermejo, y norte de la provincia de Salta. Los resultados evidenciaron diferencias significativas en longitud, ancho y peso de semillas entre las distintas procedencias. Entre ellas, el mayor coeficiente de variación lo presenta el peso de 1000 semillas, lo cual indica que este es el parámetro que más difiere entre las poblaciones analizadas.

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