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Natural additives as a source of antioxidants improve lipid oxidation, antioxidant activity, and shelf-life of beef
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Ornaghi, M.G

; Passetti, R.A.C.

; Cardoso, M.A.P.

; Vital, A.C.P.

; do Prado, R.M.

; Guerrero, A.

; Rossoni, D.F.

; do Prado, I.N.

South African Journal of Animal Science

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2024, Volume 54 N. 1 Pages 58 - 68

Abstract: En | Text: En | PDF: En

Forty young bulls were finished in feedlot with diets that contained different dosages of a combination of several natural additives (NA), i.e., clove essential oil, cashew and castor oil, and rumen-protected eugenol, vanillin, and thymol. The animals were randomized in five diets containing four different inclusion levels of NA and a control diet (n = 8 animals per treatment): basal diet without NA (CON); NA15 = 1500 mg/day; NA30 = 3000 mg/day; NA45 = 4500 mg/day, and NA60 = basal diet with 6000 mg/day of the natural additives blend. Colour, antioxidant activity (DPPH, ABTS and FRAP assays), lipid oxidation, and visual acceptability were evaluated through display (until 14 d in vacuum or film packages). Both factors (diet and display) affected all parameters evaluated. The highest dosage, NA60, was able to improve the antioxidant potential, decreasing beef oxidation to produce a higher visual acceptability. The results of this research provide evidence that NA included in the diet of beef cattle can improve overall meat quality and extend shelf-life, thus, providing higher visual acceptability through the colour perception of consumers.

Characterization of Flavonoids Profiles in Polar Extracts from Croton grewioides Baill. Using Ultra-High Resolution Mass Spectrometry Baill UltraHigh Ultra High
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Nascimento, Pedro E. S. do

; Prado, Vilma M. J.

; Jesus, Raphael A. de

; Silva, Wenes R. da

; Wisniewski Jr., Alberto

; Sá Filho, José C. F.

; Blank, Arie F.

; Souza, Daniel A. de

; Wartha, Elma R. S. A.

; Nogueira, Paulo C. L.

; Moraes, Valéria R. S.

Journal of the Brazilian Chemical Society

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jul 2023, Volume 34 N. 7 Pages 937 - 948

Abstract: En | Text: En | PDF: En

Research to identify plant bioactive compounds led to the evolution of extraction methods. This study optimized ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) to extract the flavonoids of Croton grewioides Baill. species contributing to characterize the polar extract profile and understand the antioxidant potential of these plant constituents. The use of experimental design and statistical treatments enabled the determination of the ideal conditions for each technique. The extracts of five accessions of C. grewioides were obtained under the optimized extraction conditions and were analyzed using ultra high-resolution mass spectrometry (FT-Orbitrap MS) operating at negative ionization mode for flavonoids detection. The accurate experimental mass obtained to the main compounds was used to attribute the molecular formula. Chemical structures of the main compounds detected were proposed using structure data bases. Chemometric analysis were performed with two FT-Orbitrap MS spectra samples using the identified metabolites and, the antioxidant activity data, showing that for this species the MAE was most effective in extracting the antioxidant compounds. It was possible to propose the structures for forty compounds in the C. grewioides extracts, demonstrating the excellent performance of the FT-Orbitrap MS in providing information on the chemical profile of polar compounds in plant extracts. methods ultrasoundassisted ultrasound assisted UAE (UAE microwaveassisted microwave (MAE Baill constituents technique C highresolution high resolution FTOrbitrap FT Orbitrap detection formula bases

https://doi.org/10.21577/0103-5053.20230004

In vitro evaluation of membranes for regenerative procedures against oral bacteria
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Gil, Ana Clara Kuerten

; Prado, Maick Meneguzzo

; Rocha, Laura Rhoden da

; Benfatti, César

; Schuldt Filho, Guenther

; Almeida, Josiane de

Brazilian Dental Journal

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jun 2023, Volume 34 N. 3 Pages 57 - 65

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract The current literature on guided bone regeneration (GBR) and guided tissue regeneration (GTR) membrane contamination reports that the physicochemical characteristics of these biomaterials might influence affinity to bacteria, which appears to be a major drawback for the clinical outcome of the regenerative procedures. Thus, this study aimed to evaluate, in vitro, a multispecies biofilm adherence and passage of bacteria through different types of commercially available membranes for GTR/GBR. Four types of membranes were tested (n=12): LC) Lumina Coat®; JS) Jason®; BG) Biogide®; and LP) Lumina PTFE®. Aluminum foil (AL) simulated an impermeable barrier and was used as the control. The membranes were adapted to specific apparatus and challenged with a mixed bacterial culture composed of A. actinomycetemcomitans b, S. mutans, S. mitis, and A. israelii. After 2 h or 7 days, bacterial adhesion and passage of bacteria were evaluated through CFU counting, which was analyzed by two-way ANOVA e post hoc Tukey, at a 5% significance level. Representative areas of two membranes of each group were analyzed through scanning electron microscopy (SEM) to assess the morphology and organization of the biofilm over the membrane fibers. LC and LP presented similar values of adhered bacterial cells (p > 0.05), significantly inferior when compared to the other groups, in both time points (p < 0.05). All the tested groups were permeable to bacterial cells, with no significant difference between the trial period of 2 h and 7 days (p > 0.05). SEM analyses demonstrated that adhered bacteria number increased throughout the time points (2 h < 7 days). Commercially available biological membranes demonstrated intense bacterial adherence and passage of bacteria, which increased throughout the trial period.

Resumo O objetivo deste estudo foi avaliar, in vitro, a aderência do biofilme multiespécie e a passagem de bactérias através dos diferentes tipos de membranas disponíveis comercialmente para RTG/ROG. Quatro tipos de membranas foram testados (n=12): LC) Lumina Coat®; JS) Jason®; BG) Biogide®; e LP) Lumina PTFE®. Papel alumínio (AL) simulou uma barreira impermeável e foi usado como controle negativo. As membranas foram adaptadas à um aparato específico e desafiadas com uma cultura bacteriana mista composta de A. actinomycetemcomitans b, S. mutans, S. mitis, e A. israelii. Após 2 h ou 7 dias, a aderência e passagem bacteriana foi avaliada através da contagem de UFCs. Duas membranas de cada grupo foram analisadas através da microscopia eletrônica de varredura (MEV). LC e LP apresentaram valores semelhantes de células bacterianas aderidas (p < 0.05), significativamente inferiores quando comparados aos outros grupos, em ambos os períodos experimentais (p < 0.05). Desde a análise inicial, todos os grupos testados foram permeáveis às células bacterianas, sem diferença significativa entre o período experimental de 2 h e 7 dias (p > 0.05). As análises em MEV demonstraram que o número de bactérias aderidas aumentou com o tempo (2 h < 7 days). Membranas biológicas comercialmente disponíveis demonstraram intensa aderência bacteriana e passagem de bactérias, que aumentou durante os períodos experimentais.

https://doi.org/10.1590/0103-6440202305060

Catalysts Derived from Thermal Treatments of Hydrocalumite-Type Compounds: Synthesis and Application in Transesterification Reactions
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Prado, Roberta G.

; Corrêa, Addila G. S.

; Rodrigues, Joyce F.

; Scaldaferri, Cristiane A.

; Sousa, Fabiana P. de

; Pasa, Vânya M. D.

; Constantino, Vera R. L.

; Pinto, Frederico G.

; Tronto, Jairo

Journal of the Brazilian Chemical Society

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jan 2023, Volume 34 N. 1 Pages 2 - 12

Abstract: En | Text: En | PDF: En

Catalyst performance is a relevant factor in biodiesel production because it can affect the efficiency of the process. This work provides a study of the performance of heterogeneous catalysts for biodiesel production derived from thermal-treated hydrocalumite-type compounds. This work aimed to synthesize and characterize hydrocalumite-type compounds intercalated with chloride and nitrate, evaluate the effect of thermal treatments at different temperatures (500, 600, and 750 °C) on these materials, and assess the performance of these materials as catalysts in the transesterification reactions using soybean oil and methanol. The materials were characterized by X-ray diffraction (XRD), vibrational spectroscopy (Fourier transform infrared with attenuated total reflectance (FTIR-ATR)), thermogravimetric analysis coupled to mass spectrometry (TGA DSC-MS), specific surface area, and X-ray photoelectron spectroscopy (XPS). The results of the transesterification reactions showed that the thermal-treated materials from the hydrocalumite intercalated with chloride presented high catalytic activity, among them, the thermal-treated material at 750 °C presented the highest conversion to methyl esters.

https://doi.org/10.21577/0103-5053.20220082

Normalized difference vegetation index for desiccation evaluation with glyphosate + 2,4-D in magnetized spray solution
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Alvarenga, C. B.

; Mundim, G. S. M.

; Santos, E. A.

; Gallis, R. B. A.

; Zampiroli, R.

; Rinaldi, P. C. N.

; Prado, J. R.

Brazilian Journal of Biology

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2023, Volume 83 elocation e246579

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo A magnetização da água e o geoprocessamento são ferramentas cada vez mais utilizadas no manejo de ervas daninhas. Nosso objetivo foi estudar a influência da magnetização da água na eficiência do herbicida e verificar se existe uma relação entre os escores de controle e o índice de vegetação por diferença normalizada (NDVI). No experimento de laboratório, a água foi submetida à magnetização e avaliada em relação a quatro características. No experimento de campo, parcelas de capim-braquiária foram submetidas a tratamentos em esquema fatorial (6 × 2 + 1). Seis fatores herbicidas (doses de glyphosate e glyphosate + 2,4-D) e a magnetização ou ausência de magnetização da calda foram avaliados e comparados com o tratamento controle (sem pulverização). Avaliações de controle de plantas daninhas foram realizadas seis vezes. As imagens foram obtidas usando uma câmera multiespectral incorporada para determinar os valores de NDVI. Os dados relacionados às características da água foram analisados por meio do teste t. O controle de plantas daninhas e os dados de NDVI foram submetidos à análise de variância e são apresentados em gráficos de regressão. A análise de dispersão dos dados NDVI foi realizada de acordo com os escores de controle. O processo de magnetização diminuiu o pH da água e aumentou a tensão superficial, mas não influenciou nos escores de controle ou no NDVI. Conforme a dose de glyphosate foi aumentada, os escores de controle foram maiores e os valores de NDVI foram menores. A água magnetizada não afetou a eficiência biológica dos herbicidas, e houve uma forte correlação entre os escores de controle e os valores de NDVI.

Abstract Water magnetization and geoprocessing are increasingly utilized tools in weed management. Our objective was to study the influence of water magnetization on herbicide efficiency and to verify whether there is a relationship between control scores and the normalized difference vegetation index (NDVI). In the laboratory experiment, water was subjected to magnetization and evaluated with respect to four characteristics. In the field experiment, plots of Brachiaria grass were subjected to treatments in a factorial scheme (6 × 2 + 1). Six herbicidal factors (doses of glyphosate and glyphosate + 2,4-D) and the magnetization or absence of magnetization of the spray solution were evaluated and compared against the control treatment (without spraying). Weed control assessments were carried out six times. Images were obtained using an embedded multispectral camera to determine the NDVI values. Data related to water characteristics were analyzed using the t test. Weed control and NDVI data were subjected to analysis of variance and are presented in regression graphs. Dispersion analysis of NDVI data was performed according to the control scores. The magnetization process decreased the pH of the water and increased the surface tension, but it did not influence the control scores or the NDVI. As the glyphosate dose was increased, the control scores were higher and the NDVI values were lower. Magnetized water did not affect the biological efficiency of the herbicides, and there was a strong correlation between the control scores and the NDVI values.

https://doi.org/10.1590/1519-6984.246579 540 downloads

Performance of EPDM Composites Under Thermal Plasma Ablative Tests
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Miranda, F. S.

; Prado, E. S. P.

; Silva, R. J.

; Ribeiro, A. M.

; Caliari, F. R.

; Calciolari, F. L.

; Sobrinho, A. S. Silva

; Petraconi, G.

Materials Research

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2023, Volume 26 elocation e20220326

Abstract: En | Text: En | PDF: En

Abstract In this work, a thermal plasma-based ablation test system was used to evaluate the ablative performance of the EPDM composite. The system produces a high enthalpy plasma jet generated by a plasma (DC) torch, operating at atmospheric pressure using compressed air as working gas, enabling the variation of the thermal flux concerned with the studied EPDM composites. The samples were characterized using Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS), Fourier-Transform Infrared spectroscopy (FTIR), and Thermogravimetric Analysis (TGA) to investigate the morphology, mass-loss rate, the reaction layer (char formation), and chemical changes of the samples for each thermal flux. For a complete evaluation, the thermal fluxes were varied in 0.30, 0.45, 0.60, 0.75, and 0.90 MW/m2 and for each thermal flux, disk-shape samples remained exposed to the plasma jet for 10s. During the plasma jet exposure time, the temperatures of the surface and the back of the samples were collected to verify the formed char layer's insulator capacity and the samples' thermal diffusivity for each experimental condition. The mass loss is continuous under the thermal fluxes of 0.30 and 0.45 MW/m2, stabilizing at 60% until 0.75 MW/m2. The formed char layer begins to lose its protective capacity, evidenced by the size decrease (from 800 µm to 700 µm), due to the ablation process of the reaction layer from the thermal flux of 0.90 MW/m2.

https://doi.org/10.1590/1980-5373-mr-2022-0326

Ecological importance of lepidopteran defoliators on eucalyptus plantations based in faunistic and natural enemy analyses
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Ghiotto, T. C.

; Barbosa, M. C.

; Guerreiro, J. C.

; Prado, E. P.

; Masson, M. V.

; Tavares, W. S.

; Wilcken, C. F.

; Zanuncio, J. C.

; Ferreira-Filho, P. J.

Brazilian Journal of Biology

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2023, Volume 83 elocation e268747

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract Areas planted with Eucalyptus urophylla S.T. Blake variety platyphylla F. Muell. (Myrtaceae) expand annually in most regions of Brazil. Many lepidopteran species defoliate this plant, but with damage varying per species. The objective of this study was to identify the pest status of lepidopteran defoliators based in the faunistic analysis of these insects and of their natural enemies on E. urophylla variety platyphylla plantations in a representative producing region of Brazil. Adult moths of lepidopterans and of their natural enemies were captured using a light trap, installed every two weeks, from September 2016 to August 2018. A total of 183, 10, three and 139 lepidopteran species was captured and classified as primary, secondary, without defined importance to eucalypt plants and non-identified with 1,419, seven, 465 and 876 individuals, respectively. Two primary pest species were constants, two accessories and six accidentals and all secondary ones were accidentals. Six primary pest species were common and dominant and four non-dominants. Faunistic indices indicated the main lepidopteran species that should be monitored in pest management programs. Seven hymenopteran species (65 individuals), three dipterans (49 individuals) and two hemipterans (four individuals) were the natural enemies collected using light traps. The monitoring of lepidopteran pests with light traps can contribute to the management and to reduce damage and control costs for these species, besides identifying natural enemies for biological control programs in Eucalyptus plantations.

Resumo Áreas plantadas com Eucalyptus urophylla S.T. Blake variedade platyphylla F. Muell. (Myrtaceae) se expandem anualmente na maioria das regiões do Brasil. Muitas espécies de lepidópteros desfolham esta planta, mas com danos que variam de acordo com a espécie. O objetivo deste estudo foi identificar o status de praga de lepidópteros desfolhadores com base na análise faunística desses insetos e de seus inimigos naturais em plantios de E. urophylla variedade platyphylla em uma região produtora representativa do Brasil. As mariposas e seus inimigos naturais foram capturadas com armadilha luminosa, instalada quinzenalmente, de setembro de 2016 a agosto de 2018. Um total de 183, 10, três e 139 espécies de lepidópteros foram capturadas e classificadas como primária, secundária, sem importância definida para eucaliptocultura e não identificadas com 1419, sete, 465 e 876 indivíduos, respectivamente. Duas espécies de pragas primárias foram constantes, duas acessórias e seis acidentais e todas as secundárias foram acidentais. Seis espécies de pragas primárias foram comuns e dominantes e quatro não dominantes. Os índices faunísticos indicaram as principais espécies de lepidópteras que devem ser monitoradas em programas de manejo de pragas. Sete espécies de himenópteros (65 indivíduos), três dípteros (49 indivíduos) e dois hemípteros (quatro indivíduos) foram os inimigos naturais coletados com armadilha luminosa. O monitoramento de lepidópteros-praga com armadilha luminosa pode contribuir para o manejo e redução de danos e custos de controle para essas espécies, além de identificar inimigos naturais para programas de controle biológico em cultivos de eucalipto.

https://doi.org/10.1590/1519-6984.268747

Analysis of fungal microbiota of ambient air in an intensive care unit in Rio Branco, Acre, Western Amazon, Brazil
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Ribeiro, M. A. L.

; Ribeiro, S. A. L.

; Prado, P. R.

; Prolo Júnior, S. L.

; Carvalho, C. M.

; Meneguetti, D. U. O.

; Ribeiro, M. A. L.

Brazilian Journal of Biology

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2023, Volume 83 elocation e272141

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract Fungi exhibit three adverse effects on human health: inflammatory, allergic and toxic effects, these implications affect mainly immunodepressed patients. The objective of this work was to analyze the fungal microbiota of the ambient air of an Intensive Care Unit. Three collections were carried out in an Intensive Care Unit in the city of Rio Branco, Acre, Western Amazon, Brazil from March to May 2017. 126 Petri dishes were exposed with the culture medium Agar Sabouraud with chloramphenicol and Agar Mycosel, considering the distribution of the 21 air conditioners, split residential model. The plates were incubated for seven days at room temperature and the growth of Colony Forming Units was observed. Colony counting and isolation for the morphological characterization of the granted fungi was performed. After quantification, the concentration of fungi per cubic meters of air (CFU.m-3) was settled. The third collection had a larger number of colony forming units with 48.6%. In the total of the analyzed samples, filamentous fungi (85.5%) and yeasts (14.5%) were isolated. Thirteen genera of fungi were identified, with the most frequent filaments being Cladosporium spp. 33.0%, Aspergillus spp. 30.4% and Penicillium spp. 19.6%, and yeasts Candida spp. 52.6%, Trichosporon spp. 36.9%. The colony-forming unit per cubic meter (CFU.m-3) did not shown any difference between the Cores in the same collection period, however in the 1st and 3rd collection, Core 1 had the highest average. The fungal microbiota of this Unit presented thirteen different genera potentially pathogenic, revealing the need for monitoring microorganisms and prevention actions.

Resumo Os fungos exibem três efeitos adversos para a saúde humana: inflamatórios, alérgicos e tóxicos, esses efeitos atingem principalmente pacientes imunodeprimidos. O objetivo do trabalho foi analisar a microbiota fúngica do ar ambiente em uma Unidade de Terapia Intensiva na cidade de Rio Branco, Acre, Amazônia Ocidental, Brasil. Foram realizadas 03 coletas em uma Unidade de Terapia Intensiva na cidade de Rio Branco, Acre, Amazônia Ocidental, Brasil no período de março a maio de 2017. Foram expostas 126 placas de Petri com os meios de cultura Ágar Sabouraud com cloranfenicol e Ágar Mycosel, considerando a distribuição dos 21 condicionadores de ar, modelo split residencial. As placas foram incubadas por sete dias em temperatura ambiente e observado o crescimento das Unidades Formadoras de Colônias. A contagem de colônias e o isolamento para a caracterização morfológica dos fungos isolados foi realizada. Após a quantificação, foi calculada a concentração de fungos por metros cúbicos de ar (UFC.m-3). A terceira coleta teve maior quantidade de unidades formadoras de colônias com 48,6%. No total das amostras analisadas, foram isolados fungos filamentosos (85,5%) e leveduriformes (14,5%). Foram identificados 13 gêneros de fungos, sendo os filamentosos mais frequentes Cladosporium spp. 33,0%, Aspergillus spp. 30,4% e Penicillium spp. 19,6% e os leveduriformes Candida spp. 52,6%, Trichosporon spp. 36,9%. A unidade formadora de colônia por metros cúbicos (UFC.m-3) não houve diferença entre os Núcleos no mesmo período de coleta, porém na 1ª e 3ª coleta, o Núcleo 1 apresentou maior média. A microbiota fúngica desta Unidade apresentou treze gêneros diferentes potencialmente patogênicos, mostra a necessidade de monitoramento dos microrganismos e ações de prevenção.

https://doi.org/10.1590/1519-6984.272141

Crambe cake protein as a replacement for soybean meal protein: Intake, digestibility, and ruminal parameters in lamb diets
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Poveda-Parra, A.R.

; Pereira, E. S.

; Prado-Calixto, O. P.

; Guerra, G. L.

; Pires, K. A.

; Lopes, M. R.

; Rodrigues, E. R.

; Cavalheiro Junior, E. R.

; do Carmo, J. P. M.

; Mizubuti, I. Y.

South African Journal of Animal Science

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2022, Volume 52 N. 6 Pages 873 - 885

Abstract: En | Text: En | PDF: En

The objective was to evaluate different levels of crambe cake protein (0, 250, 500, 750 and 1000 g/kg of DM) to replace soybean meal protein on the intake, apparent digestibility coefficient (ADC), blood glucose, ruminal fermentation, and protozoal population in lambs. Two experiments were performed. In experiment one, 40 crossbred male lambs weighing 20 ± 3.45 kg were used, distributed in a completely randomized design, to study intake and ruminal parameters. In experiment two, 25 crossbred male lambs weighing 23.44 ± 1.04 kg were used to study digestibility. The intake of dry matter and total carbohydrate (TC) (kg/lambs/day and g/kg0.75), crude protein (kg/lambs/day) and final body weight decreased with increase of crambe cake protein as a replacement for soybean meal protein, while ether extract ( EE) intake (kg/lambs/day and g/kg075) increased. The ADC of EE presented a positive linear effect and the ADC of TC and non-fibrous carbohydrates presented a negative linear effect. There was no interaction within treatments and collection period (0, 3, 6 and 9 h) on ruminal fluid characteristics. Blood glucose presented a quadratic effect for levels of crambe cake protein in diets (max. P = 35.18%), and linear increase for collection period. Ruminal pH showed a quadratic effect for sampling hours (max. P = 5.43 h). For protozoa evaluation, the total amount, Entodinium, and Eudiplodium presented linear decreasing effects with treatment, while Isotricha, Eudiplodium, Ostracodinium and the total amount of protozoa had a linear decrease with sampling hours. Soybean meal protein can be replaced by crambe cake protein at up to 250 g/kg DM.

https://doi.org/10.4314/sajas.v52i6.13

10.

Evaluation of the Seasonality and Extraction Method on the Polar Extracts of Croton grewioides Baill. by Chromatogram Fingerprinting and Isolation of a New Triglycosylated Flavonoid
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Prado, Vilma M. J.

; Jesus, Raphael A. de

; Oliveira, Julio M. A.

; Pereira, Camila S. A.

; Blank, Arie F.

; Pereira-Filho, Edenir R.

; Cass, Quezia B.

; Lima, Juliana M. de

; Ferreira, Antonio G.

; Nogueira, Paulo C. L.

; Moraes, Valéria R. S.

Journal of the Brazilian Chemical Society

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feb 2021, Volume 32 N. 2 Pages 385 - 394

Abstract: En | Text: En | PDF: En

Croton grewioides Baill. popularly known as “canelinha” or “canelinha-de-cheiro” has been used for the treatment of influenza, antitussive, febrifuge and headache; however, the study of its phytochemical composition is limited. The aim of this study was to investigate the effect of the extraction method and seasonality through leaf extracts of four accessions of Croton grewioides by fingerprint chromatograms aided by principal component analysis to analyze the differences and similarities among the samples. We aimed also to provide chemical characterization of isolated secondary metabolites using semi-preparative liquid chromatography. The results showed that only the chemical profile of the methanolic extracts of accessions 101 and 113 were influenced by the seasonality. For the first time, four flavonoids were isolated through semi-preparative chromatography in this species, characterized as quercetin 3-O-β-D-galactopyranosyl-(1→2)-α-L-rhamnopyranoside-(1→6)-α-L-rhamnopyranoside (1), quercetin 3-O-β-D-galactopyranosyl-(1→2)-α-apiopyranoside-(1→6)-α-L-rhamnopyranoside (2), quercetin 3-O-glucopyranoside (3) and 3-O-methyl-quercetin (4), the flavonoid (2) has been recognized as a new triglycosylated derivative.

https://doi.org/10.21577/0103-5053.20200190 249 downloads

11.

Assessing Genetic Variation and Spread of Phalaris minor Resistant to ACCase Inhibiting Herbicides in Iran
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GHEREKHLOO, J.

; ALCÁNTARA-DE LA CRUZ, R.

; OSUNA, M.D.

; SOHRABI, S.

; PRADO, R.

Planta Daninha

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2020, Volume 38 elocation e020195618

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: A erva-cabecinha (Phalaris minor) é a segunda planta daninha mais problemática nos campos de trigo do Irã, pois tem desenvolvido resistência aos herbicidas inibidores da ACCase. Marcadores moleculares de intersequência repetitiva simples (ISSR) foram utilizados para avaliar a variação genética entre e dentro de populações resistentes e suscetíveis aos inibidores de ACCase de P. minor do Irã, a fim de elucidar a origem da resistência e sua dispersão. Dezesseis populações de P. minor de diferentes regiões do Irã foram analisadas usando sete primers ISSR. As relações genéticas obtidas a partir da análise UPGMA revelaram a presença de mais de um genótipo entre as populações resistentes aos herbicidas. Os resultados indicaram que a alta similaridade genética e proximidade física entre as populações resistentes de P. minor nas diferentes regiões foi devido principalmente à polinização cruzada, à dispersão mecânica de sementes e a fatores ecológicos locais. Assim, infere-se que ocorreu uma seleção independente, assim como a movimentação de sementes resistentes, explicando a ocorrência e dispersão da resistência nessas populações.

ABSTRACT: Littleseed canarygrass (Phalaris minor) is the second most serious and problematic grass weed in wheat fields in Iran, and has developed resistance to ACCase inhibiting herbicides. Inter-simple sequence repeat (ISSR) analysis was used to assess genetic variation between and within ACCase inhibitor-resistant and susceptible P. minor populations in Iran and to determine the origin of resistance and its dispersal. Sixteen P. minor populations from different regions in Iran were analysed using seven primers. Genetic relationships generated using UPGMA analysis indicated the presence of more than one genotype among the herbicide resistant populations. The results indicated that the high genetic similarity and physical proximity among the resistant P. minor populations in the different regions is mainly due to cross pollination, mechanical seed dispersion and local ecological factors. These findings suggested that independent selection as well as movement of resistant seeds had occurred, which could explain the presence and dispersion of ACCase inhibitor-resistance in these populations.

https://doi.org/10.1590/s0100-83582020380100026 1138 downloads

12.

Application of LC-DAD Metabolic Fingerprinting in Combination with PCA for Evaluation of Seasonality and Extraction Method on the Chemical Composition of Accessions from Lippia alba (Mill) N. E. Brown and Biological Activities
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Jesus, Raphael A. de

; Prado, Vilma M. J.

; Pinto, Vanderson S.

; Silva, Valdenizia R.

; Santos, Luciano S.

; Nogueira, Paulo C. L.

; Navickiene, Sandro

; Pereira-Filho, Edenir R.

; Blank, Arie F.

; Bezerra, Daniel P.

; Soares, Milena B. P.

; Seidl, Claudia

; Cardoso, Carmen L.

; Moraes, Valéria R. S.

Journal of the Brazilian Chemical Society

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may 2019, Volume 30 N. 5 Pages 978 - 987

Abstract: En | Text: En | PDF: En

The methodology developed in this study through the acquisition of fingerprint chromatograms by liquid chromatography-diode array detector (LC-DAD), aided by principal component analysis, made it possible to assess, in a rational way, the chemical differences between six accessions of Lippia alba and to verify the influence of the method of extraction, the seasonality and the individuality of each accession on chemical composition of its extracts. Among all extracts analyzed against cancer cell lines, eight of them showed to be more promising against a human leukemia cell line (HL-60), displaying cell growth inhibition percentage ranging between 40.0 and 52.0%. In the inhibitory activity assays against acetylcholinesterase enzyme, all extracts showed weak inhibitory effect, with highlight only for six of them, which displayed inhibition ranging between 27.0 and 32.0%.

https://doi.org/10.21577/0103-5053.20180244 666 downloads

13.

Extended-time of Noninvasive Positive Pressure Ventilation Improves Tissue Perfusion after Coronary Artery Bypass Surgery: a Randomized Clinical Trial
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Nasrala, Mara L. S.

; Bolzan, Douglas W.

; Lage, Yumi G.

; Prado, Fabiana S.

; Arena, Ross

; Lima, Paulo R. L.

; Feguri, Gibran

; Silva, Ageo M. C.

; Marcondi, Natasha O.

; Hossne, Nelson

; Guizilini, Solange

; Gomes, Walter J.

Brazilian Journal of Cardiovascular Surgery

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jun 2018, Volume 33 N. 3 Pages 250 - 257

Abstract: En | Text: En | PDF: En

Abstract Objective: To compare the effects of extended- versus short-time noninvasive positive pressure ventilation on pulmonary function, tissue perfusion, and clinical outcomes in the early postoperative period following coronary artery bypass surgery in patients with preserved left ventricular function. Methods: Patients were randomized into two groups according to noninvasive positive pressure ventilation intensity: short-time noninvasive positive pressure ventilation n=20 (S-NPPV) and extended-time noninvasive positive pressure ventilation n=21 (E-NPPV). S-NPPV was applied for 60 minutes during immediate postoperative period and 10 minutes, twice daily, from postoperative days 1-5. E-NPPV was performed for at least six hours during immediate postoperative period and 60 minutes, twice daily, from postoperative days 1-5. As a primary outcome, tissue perfusion was determined by central venous oxygen saturation and blood lactate level measured after anesthetic induction, immediately after extubation and following noninvasive positive pressure ventilation protocols. As a secondary outcome, pulmonary function tests were performed preoperatively and in the postoperative days 1, 3, and 5; clinical outcomes were recorded. Results: Significant drop in blood lactate levels and an improvement in central venous oxygen saturation values in the E-NPPV group were observed when compared with S-NPPV group after study protocol (P<0.01). The E-NPPV group presented higher preservation of postoperative pulmonary function as well as lower incidence of respiratory events and shorter postoperative hospital stay (P<0.05). Conclusion: Prophylactic E-NPPV administered in the early postoperative period of coronary artery bypass surgery resulted in greater improvements in tissue perfusion, pulmonary function and clinical outcomes than S-NPPV, in patients with preserved left ventricular function. Trial Registration: Brazilian Registry of Clinical trial - RBR7sqj78 - http://www.ensaiosclinicos.gov.br

https://doi.org/10.21470/1678-9741-2017-0232 886 downloads

14.

Studying MID-Range planar orbits around Phobos
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Cavalca, M. P. O.

; Prado, A. F. B. A.

; Formiga, J. K. S.

; Gomes, V. M.

; Sanchez, D. M.

Revista mexicana de astronomía y astrofísica

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2018, Volume 54 N. 2 Pages 429 - 442

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract The goal of the present paper is to search and study mid-range planar orbits for a spacecraft traveling near Phobos. The first step is to make a numerical search and classification of natural orbits based in the concept of “Quasi Satellite Orbits” (QSO). The effects of the eccentricity of Phobos and the irregular shape of the bodies involved (Mars and Phobos) are studied, identifying the importance of these terms. This study is made using two different initial locations for Phobos, the periapsis and apoapsis. The results show the existence of several solutions, mapping the minimum, maximum and averaged Phobos-spacecraft distances.

Resumen El objetivo de este artículo es buscar y estudiar órbitas planas intermedias para naves espaciales que viajan cerca de Phobos. El primer paso es una búsqueda numérica y clasificación de las órbitas naturales basada en el concepto de las “órbitas cuasi satelitales” (QSOs). Se estudian los efectos de la excentricidad de Phobos y de la forma irregular de Phobos y Marte, y se identifica la importancia de los términos correspondientes. Se emplean dos posiciones iniciales diferentes para Phobos, el periapsis y el apoapsis. Los resultados muestran la existencia de varias soluciones que mapean las distancias mínima, máxima y promedio entre Phobos y la nave.

273 downloads

15.

3rd Guideline for Perioperative Cardiovascular Evaluation of the Brazilian Society of Cardiology
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Gualandro, Danielle Menosi

; Yu, Pai Ching

; Caramelli, Bruno

; Marques, André Coelho

; Calderaro, Daniela

; Fornari, Luciana Savoy

; Pinho, Claudio

; Feitosa, Alina Coutinho Rodrigues

; Polanczyk, Carisi Anne

; Rochitte, Carlos Eduardo

; Jardim, Carlos

; Vieira, Carolina L. Z.

; Nakamura, Debora Y. M.

; Iezzi, Denise

; Schreen, Dirk

; Adam, Eduardo Leal

; D’Amico, Elbio Antonio

; Lima, Emerson Q. de

; Burdmann, Emmanuel de Almeida

; Mateo, Enrique Indalecio Pachón

; Marcondes-Braga, Fabiana Goulart

; Machado, Fabio S.

; Paula, Flavio J. de

; Carmo, Gabriel Assis Lopes do

; Feitosa-Filho, Gilson Soares

; Prado, Gustavo Faibischew

; Lopes, Heno Ferreira

; Fernandes, João R. C.

; Lima, José J. G. de

; Sacilotto, Luciana

; Drager, Luciano Ferreira

; Vacanti, Luciano Janussi

; Rohde, Luis Eduardo Paim

; Prada, Luis F. L.

; Gowdak, Luis Henrique Wolff

; Vieira, Marcelo Luiz Campos

; Monachini, Maristela Camargo

; Macatrão-Costa, Milena Frota

; Paixão, Milena Ribeiro

; Oliveira Junior, Mucio Tavares de

; Cury, Patricia

; Villaça, Paula R.

; Farsky, Pedro Silvio

; Siciliano, Rinaldo F.

; Heinisch, Roberto Henrique

; Souza, Rogerio

; Gualandro, Sandra F.M.

; Accorsi, Tarso Augusto Duenhas

; Mathias Júnior, Wilson

Arquivos Brasileiros de Cardiologia

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sep 2017, Volume 109 N. 3 Pages 1 - 104

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.5935/abc.20170140 100302 downloads

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