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RESUMEN Una alternativa para reducir el uso de hormonas con aplicación inmediata en el cultivo comercial de la tilapia del Nilo, es la evaluación precisa de los tiempos de masculinización en variedades domesticadas. El objetivo del trabajo fue evaluar el porcentaje de masculinización y el crecimiento obtenido en la tilapia del Nilo alimentada con alimento comercial adicionado con 17ɑ-metiltestosterona a diferentes tiempos. Para ello se evaluaron cinco tiempos (0, 10, 15, 20 y 25 días) de administración de hormona a una población mixta de tilapia del Nilo variedad Spring. Para cada tratamiento se utilizaron tres réplicas. Los alevines fueron sembrados a densidad de 0.5 alevines L-1 en un sistema de recirculación cerrado. El cultivo consistió de 30 días en acuarios de acrílico de 85 L y 20 días en tanques exteriores de 1000 L. La determinación del sexo se realizó con la técnica de squash. El crecimiento se determinó por medio del peso húmedo y la longitud total obtenida de las biometrías. Los resultados indican una masculinización del 100% desde los 10 días de aplicación del esteroide, pero los mayores valores de crecimiento se observaron en los grupos que recibieron el esteroide por mayor tiempo. Los resultados muestran que no es necesario administrar el esteroide durante 30 días para lograr una masculinización del 100% en variedades domesticadas y que el esteroide tiene efecto positivo en la tasa de crecimiento de los alevínes, persistiendo por algunas semanas después de terminado el tratamiento.

ABSTRACT An alternative to reduce the use of hormones with an immediate application to Nile tilapia commercial culture, is the precise evaluation of masculinization duration in domesticated strains. The main objective of this work was to evaluate the percentage of masculinization and growth obtained in Nile tilapia fed with 17ɑ-methyltestosterone at different times during the fry period. For this, five times (0, 10, 15, 20 and 25 days) of application of the hormonal treatment were evaluated in a mixed population of Nile tilapia’s Spring strain. For each treatment, three replicates were used. Fry were stocked at a density of 0.5 L-1 in a recirculation system. Culture consisted of 30 days in 85 L acrylic aquaria and another 20 days in 1000 L exterior tanks. Squash technique was used for determination of sex. Growth was determined from wet weight and total length obtained in the biometrics. The results obtained indicated a 100% masculinization after 10 days of steroid application; but, the highest values in terms of growth rates were observed in the groups that received the steroid for the longest time. The results show that it is not necessary that the steroid is administered for 30 days to achieve a 100% masculinization in a domesticated line and that the steroid has a positive effect on the growth of the fry which persists for a few weeks after the end of the treatment.

https://doi.org/10.19136/era.a8n1.2739 12 downloads

Dermatosis Perforante Adquirida
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Trucco, L

; Ramirez, MI

; Acciarri, ML

; Balboa, ES

; Mayora, MV

; Torres, LP

; Amoros, NS

Revista argentina de dermatología

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dec 2020, Volume 101 N. 4 Pages 21 - 30

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT Perforating disorders are a group of diseases characterized by transepidermal extrusion of dermal material, it includes reactive perforating collagenosis, perforating folliculitis, elastosis perforansserpiginosa and Kyrle's disease, and in recent years the term acquired perforating dermatosis (APD) has been used to refer to the perforating dermatoses that occur in adult patients associated with systemic disorder (kidney failure, diabetes mellitus, hypothyroidism, among others). The etiopathogenesis is still controversial and it is believed that several factors participate in the process, such as traumatism produced by chronic pruritus in predisposed patients, diabetic microangiopathy may contribute to collagen damage and to the microdeposition of substances that are no removed by dialysis, causing local inflamatory reaction. The itching constitudes the main symptom of this pathology, and clinically presents a papulo-nodules centered by keratosis crusts. Differential diagnoses include: nodular prurigo, multiple keratoacanthomas, sarcoid, and other perforating dermatosis. Treatment is difficult, with great tendency to chronicity. We report the case of a 57 year-old female patient, with history of type 2 diabetes, hypothyroidism and renal failure, with itching of several months of evolution.

RESUMEN Los trastornos perforantes constituyen un grupo de enfermedades caracterizadas por la extrusión transepidérmica de material dérmico. La etiopatogenia es desconocida, sin embargo, la forma adquirida generalmente se presenta asociada a enfermedades sistémicas como insuficiencia renal y diabetes mellitus, entre otras. El prurito es el síntoma cardinal de esta patología, y clínicamente presenta pápulo-nódulos centrados por costras queratósicas. En cuanto al tratamiento, la dermatosis perforante adquirida (DPA) es de difícil control, con tendencia a la cronicidad. Presentamos el caso de una paciente de género femenino de 57 años de edad, con antecedente de diabetes,hipertensión arterial (HTA), hipotiroidismo e insuficiencia renal, con prurito generalizado de varios meses de evolución.

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Biofertilización con Rhizoglomus irregulare y Azospirillum brasilense en Brachiaria híbrido cv. Mulato II
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González-Cañizares, Pedro J.

; Reyes-Rouseaux, Reynerio

; Fernández-Milanés, Delio

; San Juan-Rodríguez, Ana Nelis

; Dopico-Ramirez, Daisy

; Ramírez-Pedroso, Juan F.

; Fundora-Sánchez, Luis R.

Cultivos Tropicales

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sep 2020, Volume 41 N. 3 elocation e03

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT An experiment was carried out with the objective of evaluating the effect of biofertilization with the plant growth promoting bacteria Azospirillum brasilense and the arbuscular mycorrhizal fungus Rhizoglomus irregulare in Brachiaria hybrid grass cv. Mulato II cultivated in a Mollic Carbonated Cambisol. It included four treatments formed by the inoculation, alone and in combination, of both microorganisms plus a control without inoculation, in a Latin Square design. The inoculum with R. irregulare had a concentration of 30 g-1spores and was applied by the method of coating the seeds, while A. Brasilense, with a concentration of 109 CFU mL-1, was applied by embedding the seeds at the time of planting, and at a rate of 20 L ha-1after the first cut. Four cuts were made, at intervals of 60 and 90 days in the rainy and dry season, respectively. R. irregulare increased the frequency and intensity of mycorrhizal colonization, as well as the number of spores in the rhizosphere, although the greatest results were achieved when applied with A. brasilense. Biofertilizers separately increased the growth of the aerial and radical part of the plants, the nitrogen concentrations in the aerial biomass and the dry mass yield of the grass, but the higher values of these variables, as well as the greater contribution of the biofertilization in Nitrogen grass nutrition was obtained with its joint application. The effect of biofertilization was maintained for one year.

RESUMEN Se realizó un experimento con el objetivo de evaluar el efecto de la biofertilización con la rizobacteria Azospirillum brasilense y el hongo micorrízico arbuscular Rhizoglomus irregulare en el pasto Brachiaria híbrido cv. Mulato II cultivado en un suelo Pardo Mullido Carbonatado. Se incluyeron cuatro tratamientos conformados por la inoculación sola y combinada, de ambos microorganismos, más un testigo sin inocular, en un diseño cuadrado latino. El inóculo con R. irregulare poseía una concentración de 30 esporas g-1 y se aplicó mediante el método del recubrimiento de las semillas al momento de la siembra, mientras que A. brasilense, con una concentración de 109 UFC mL-1, se aplicó sumergiendo las semillas en una solución de inóculo y agua en relación 1:10, y a razón de 20 L ha1 después del primer corte. Se realizaron cuatro cortes, a intervalos de 60 y 90 días en el período lluvioso y poco lluvioso, respectivamente. R. irregulare aumentó la frecuencia e intensidad de la colonización micorrízica, así como el número de esporas en la rizosfera, aunque los mayores resultados se alcanzaron cuando se aplicó con A. brasilense. Los biofertilizantes por separado incrementaron el crecimiento de la parte aérea y radical de las plantas, las concentraciones de nitrógeno en la biomasa aérea y el rendimiento de masa seca del pasto, pero los mayores valores de estas variables, así como la mayor contribución de la biofertilización a la nutrición nitrogenada del pasto, se obtuvieron con su aplicación conjunta. El efecto de la biofertilización se mantuvo durante un año.

Atrofodermia lineal de Moulin
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Trucco, L

; Ramirez, MI

; Acciarri, ML

; Balboa, ES

; Mayora, MV

; Amoros, NS

Revista argentina de dermatología

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sep 2019, Volume 100 N. 3 Pages 93 - 100

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract It is a disease not very frequent, described in 1992 by Moulin, which occurs between 6 and 20 years of age, characterized by hyperpigmented atrophic bands distribution blaschokoide, mainly located in trunk, unilateral, that are not preceded by inflammation or changes sclerodermal, induration, or adhesions to deep. It is a self-limited condition, whose treatments are ineffective. We present the case of a boy, 12 years old, with atrophic, hyperpigmented and asymptomatic plates, distributed from the left mammary region to the homolateral back, following the lines of Blaschko. Refers that is started as a hyperpigmented macula and in recent years it has been atrophying, it is not accompanied by any symptomatology, and was not preceded by inflammatory. We performed laboratory test with complete blood count, renal function, liver function and antibody titers, which were normal; and incisional biopsy by punch, that reported, for a sample stained with hematoxylin-eosin, epidermis of variable thickness, canned, no cellular atypia or disorders madurativos. Dermis impresses discreetly thickened, with homogenization of collagen. Slight perivascular inflammatory infiltrate. With clinical and anatomopathology we arrive at the diagnosis of Linear atrophoderma of Moulin.

Resumen Es una enfermedad poco frecuente, descripta en 1992 por Moulin, que se presenta entre los 6 y los 20 años de edad, caracterizada por bandas hiperpigmentadas atróficas de distribución blaschkoide, localizadas principalmente en tronco, unilaterales, que no son precedidas por inflamación o cambios esclerodérmicos, induración ni adherencias a planos profundos. En general es una afección autolimitada, cuyos tratamientos resultan ineficaces. Presentamos el caso de un niño de 12 años, con lesiones en tronco compatibles con el diagnóstico de Atrofodermia lineal de Moulin (ALM).

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Tabaquismo: un problema que afecta a jóvenes universitarios a nivel nacional e internacional
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Correa-López, Lucy E

; Segura-Márquez, Carmen L

; Cedillo-Ramirez, Lucy

; Morales-Romero, Andrea M

; Olivera-Ruiz, Jesus E

Revista de la Facultad de Medicina Humana

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jul 2019, Volume 19 N. 3 Pages 106 - 106

Abstract: Es | Text: Es | PDF: Es

https://doi.org/10.25176/RFMH.v19i3.2147

Síndrome de Kounis, a propósito de un caso
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Méndez-Betancourt, Johan L

; Ramirez-Ramos, Cristhian F

; López-Guevara, Oscar A

; Osorio-Carmona, Hugo E

Acta Médica Peruana

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jan 2018, Volume 35 N. 1 Pages 65 - 70

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Kounis syndrome is defined as an acute coronary syndrome secondary to an allergic response; and it is also known as allergic angina - acute myocardial infarction. There is little data on the epidemiology of this disease because of having been recently described. We present the case of a 46-year old woman who developed an episode of colicky abdominal pain in the right upper quadrant for 4 days, and a diagnosis of abdominal pain of biliary origin was considered. Fifteen minutes after symptomatic therapy was administered (hyoscine and metamizole), the patient developed sudden chest pain. Physical examination revealed hypotension, tachycardia, rash, and wheezing. The electrocardiogram showed ST segment depression. The case was managed as an acute coronary syndrome, vital support and steroids were part of its management, and the patient was admitted in the intensive care unit for coronary monitoring. A few days later, the patient was uneventfully discharged

El síndrome de Kounis se caracteriza por un compromiso cardiaco que es secundario a una respuesta alérgica. Hay pocos datos acerca de la epidemiologia de esta enfermedad debido a su reciente descripción. Se presenta el caso de una paciente mujer de 46 años sin antecedentes de importancia que presentó dolor tipo cólico episódico en hipocondrio derecho de 4 días de tiempo de enfermedad, en la que se consideró el diagnóstico de cólico de origen biliar. Quince minutos luego de manejo sintomático (hiocsina/dipirona), la paciente presentó dolor torácico en región precordial de inicio súbito. La evaluación física mostró hipotensión, taquicardia, presencia de habones en todo el cuerpo y sibilantes a la auscultación pulmonar. El electrocardiograma mostro infradesnivel del segmento ST. Considerado como síndrome coronario agudo, se realizó el manejo con esteroides y soporte vital, y se indicó monitorización cardiovascular en unidad de cuidados intensivos. La paciente egresó sin complicación luego de unos días

Alterações respiratórias, auditivas e citogenéticas em trabalhadores de um estaleiro no Rio de Janeiro: estudo de caso
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Murata, Marcos Massao

; Costa-Amaral, Isabele Campos

; Carvalho, Leandro Vargas Barreto de

; Souza, Gabriela Rodrigues de

; Mainenti, Helena Ramirez Domingos

; Carvalho, Márcia Aparecida Ribeiro de

; Valente, Daniel

; Coutinho, Gilvania Barreto Feitosa

; Vale, Diane Francis Ribeiro do

; Rodrigues, Camila do Nascimento

; Ribeiro, Patrícia Canto

; Oliveira, Monica Stuck de

; Menezes, Marco Antônio Carneiro

; Teixeira, Liliane Reis

; Larentis, Ariane Leites

; Moreira, Maria de Fátima Ramos

; Almeida, Márcia Soalheiro de

; Castro, Hermano Albuquerque de

; Mattos, Rita de Cássia Oliveira da Costa

Cadernos Saúde Coletiva

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dec 2017, Volume 25 N. 4 Pages 394 - 404

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Introdução Este estudo avaliou alterações respiratórias, auditivas e citogenéticas em trabalhadores de um estaleiro em Angra dos Reis, no Estado do Rio de Janeiro, relacionadas à exposição de pintores a solventes, metais e ruído no ambiente de trabalho. Métodos Foram avaliados função pulmonar, perdas auditivas e índice de reconhecimento da fala, e alterações citogenéticas pelo teste de aberrações cromossômicas. Foram avaliados manganês e chumbo em sangue por espectrometria de absorção atômica. Os indicadores de efeito utilizados para chumbo foram ALAD e ALA-U, determinados por espectrofotometria e cromatografia líquida, respectivamente. Resultados Seis dos 9 trabalhadores avaliados apresentaram alteração funcional respiratória. Quase 70% dos 18 trabalhadores avaliados apresentaram audição reduzida, com associação entre PAIR e chumbo em sangue. O percentual médio de recuperação da ALAD foi de 32,9%, com médias de ALA-U de 1,7 mg g-1 creatinina, 4,65 µg dL-1 para Pb-S e 10 µg L -1 para Mn-S entre os trabalhadores. Foram observadas associações entre ALA-D ativada com Mn-S e com a presença de aberrações cromossômicas. As alterações citogenéticas identificadas foram aneuploidias, separação prematura centromérica e aberrações como formação de anéis, quebras e união de cromátides irmãs. Conclusão os trabalhadores do estaleiro apresentam alterações que podem ser associadas à exposição ocupacional.

Abstract Introduction This study evaluated respiratory, audiological and cytogenetic alterations in shipyard workers in Angra dos Reis, state of Rio de Janeiro/Brazil, and their correlation to the occupational exposure of painters to solvents, metals and noise present. Methods We evaluated pulmonary function, hearing loss and speech recognition, as well as cytogenetic alterations. Indicators of exposure to lead and manganese in blood were evaluated by atomic absorption spectrometry. The determination of ALAD and ALA-U was performed by spectrophotometry and liquid chromatography, respectively. Results Six of the 9 workers evaluated for lung function had respiratory functional impairment. Almost 70% of the 18 evaluated workers had reduced hearing, with association between PAIR and values of lead in blood. The average percentage of recovery of ALAD was 32.9%; average was 1.7 mg g-1 creatinine for ALA-U, 4.65 µg dL-1 for Pb-B and 10 µg L-1 for Mn-B. We observed associations between activated ALA-D with Mn-B and the presence of chromosomal aberrations. Furthermore, we identified cytogenetic alterations as aneuploidy, premature centromere separation; as well as ring formation, breakage, and sister chromatid union. Conclusion Shipyard workers presented alterations that can be associated with occupational exposure.

https://doi.org/10.1590/1414-462x201700040324 1456 downloads

Estado y tendencia de la Educación Nuclear en el Estado Plurinacional de Bolivia
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Bustos Espinoza, R. O. E

; Burgoa Mariaca, A

; Poma Mamani, I

; Ticona Peralta, R. D

; Subieta Vasquez, M

; RamIrez Ávila, G. M

; Raljevic Ergueta, M

; Lucano, M

; Mamani, R

; Vargas Lucana, M

; Chavez Ríos, S

; Torrico Ferrufino, S

; Coca Valdez, D

; Romero Bolaños, L

Revista Boliviana de Física

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nov 2017, Volume 30 N. 30 Pages 31 - 55

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

El Programa Nuclear Boliviano tiene el objetivo de promover, dirigir, implementar y difundir el uso pacífico de la tecnologıa nuclear en el país así como actualizar y apoyar a los sectores industrial, científico, tecnologico y académico. En ese sentido el gobierno nacional planifica la Construccion e Implementación del Centro de Investigación y Desarrollo en Tecnología Nuclear, cuyo operador sera la Agencia Boliviana de Energía Nuclear - ABEN. Asimismo, el Instituto Boliviano de Ciencia y Tecnología Nuclear - IBTEN, autoridad nacional competente en materia de uso de radiaciones ionizantes, sera la entidad que tendrá a su cargo el licenciamiento para las fases de construccion, operación y cierre de dichas instalaciones. En este marco institucional, las universidades se sumaran al proyecto mediante la realizacion de investigaciones, tanto teóricas como experimentales, así como tambien en fortalecer programas de formacion académica ya establecidos, vinculados al área nuclear. Existen programas que fortalecen el estudio del area nuclear, como ser: la Olimpiada Boliviana de Física, la Olimpiada Boliviana de Astronomıa y Astrofísica, en coordinacion con el Ministerio de Educacion. Por otro lado, la Carrera de Física de la UMSA cuenta con el programa del Diplomado en Física para profesores de Colegio, buscando el mejoramiento continuo del sistema educativo boliviano en estas disciplinas: Física, Astronomía y Astrofísica y sus directas transversales: Biologıa, Química, Matematica, Geología, Ciencias Sociales, etc.

Bolivias National Nuclear Program has as its objective to promote, manage, and implement the pacific use of nuclear technology in Bolivia, as well as, to bring up to date and support the countrys industrial, scientific, technological and academic sectors. The Plurinational State of Bolivia has put in place the construction and implementation of the Bolivian Research and Development Center for Nuclear Technology under the administration of The Bolivian Agency for Nuclear Energy (ABEN). This project will also involve the Bolivian Institute of Nuclear Science and Technology (IBTEN) which will oversee the construction and operation of the Nuclear Center. Within this context Bolivian universities are stepping up research, both experimental and theoretical, as well as, strengthening existing training programs related to nuclear science and technology. Academics in coordination with the Ministry of Education have been promoting and implementing programs that strengthen studies in the field of nuclear physics through activities such as the Bolivian Physics Olympics and the Bolivian Astronomy and Astrophysics Olympics. In addition the Physics Department of UMSA implements a Physics Diploma program for school teachers. The program seeks to update teachers in disciplines, such as, physics, astronomy, astrophysics and also biology, chemistry, mathematics, social sciences, amongst others within the Bolivian education system.

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10.

Fenólicos a partir de residuos de café: Optimización del proceso de extracción
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Marcelo-Diaz, R

; Luján-Gonzales, V

; Ramirez, L

; Olano, M

; Vargas, A

; Rojas, M.L

; Linares, G

Revista de Investigaciones Altoandinas

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oct 2017, Volume 19 N. 4 Pages 405 - 410

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT The coffee industry, coffee shops and restaurants are in constant expansion and growth. Consequently, large amounts of Spent Coffee Grounds (SCG) are generated, which are solid coffee residues obtained after the ground coffee leaching process. This residue can be reused because of its compounds as phenolics. The objective of the present study was to evaluate the effect of extraction time (30-90 min) assisted by ultrasound (45kHz and 50 W), liquid-solid ratio (10-40 ml/g) and ethanol concentration (20-93.8%) in the total phenolic compounds (CFT) content expressed as mg gallic acid/g SCG dry matter. Surface response methodology (MSR) was used, as an eﬃcient technique that minimizes the number of experiments, through the central rotational compound design (DCCR) to determine the effect of these 3 factors and to perform the optimization of the extraction process conditions of total phenolic compounds. The predictions of the model were correlated with the experimental values in 84.04%, where there was a strong influence (P <0.05) of the ultrasound-assisted extraction time variables and liquid- solid ratio in the results. Optimal conditions (21.03% ethanol at a liquid-solid ratio of 11.65 ml/g SCG and ultrasonic assisted processing for 32.42 minutes) allowed a total phenolic compound content of 1429.09 mg AG/g of SCG to be obtained, a content higher than Obtained by other extraction techniques reported in the literature.

RESUMEN La industria cafetera, cafeterías y restaurantes se encuentran en constante expansión. En consecuencia, se generan grandes cantidades de residuos sólidos de café - Spent Coffe Grounds (SCG), que son obtenidos después del proceso de lixiviación del café molido. Este residuo puede ser reutilizado debido a sus compuestos fenólicos. El objetivo del presente estudio fue evaluar el efecto del tiempo de extracción (30-90 min) asistido por ultrasonido (45 kHz y 50 W), relación líquido-sólido (10-40 ml/g) y concentración de etanol (20-93.8%) en el contenido de compuestos fenólicos totales (CFT) expresado en mg de ácido gálico/g de SCG en materia seca. Se utilizó la metodología de respuesta superficial (MSR), como una técnica eficiente que minimiza el número de experimentos, a través del diseño del rotacional central compuesto (DCCR) para determinar el efecto de estos 3 factores y para optimizar las condiciones del proceso de extracción de compuestos fenólicos totales compuestos. Las predicciones del modelo se correlacionaron con los valores experimentales en 84.04%, donde hubo una fuerte influencia (p <0,05) de las variables de tiempo de extracción asistida por ultrasonido y la relación líquido-sólido en los resultados. Las condiciones óptimas de 21.03% de etanol en una relación lıquido-sólido de 11.65 ml/g de SCG y procesamiento asistido por ultrasonidos durante 32.42 min, permitieron obtener un contenido de compuesto fenólico total de 1429.09 mg AG/g de SCG. Este contenido fué mayor al obtenido por otras técnicas de extracción descritas en la literatura

https://doi.org/10.18271/ria.2017.315

11.

Serological based monitoring of a cohort of patients with chronic Chagas disease treated with benznidazole in a highly endemic area of northern Argentina
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Niborski, Leticia L

; Grippo, Vanina

; Lafón, Sonia O

; Levitus, Gabriela

; García-Bournissen, Facundo

; Ramirez, Juan C

; Burgos, Juan M

; Bisio, Margarita

; Juiz, Natalia A

; Ayala, Vilma

; Coppede, María

; Herrera, Verónica

; López, Crescencia

; Contreras, Ana

; Gómez, Karina A

; Elean, Juan C

; Mujica, Hugo D

; Schijman, Alejandro G

; Levin, Mariano J

; Longhi, Silvia A

Memórias do Instituto Oswaldo Cruz

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jun 2016, Volume 111 N. 6 Pages 365 - 371

Abstract: En | Text: En | PDF: En

This study aimed to evaluate well-documented diagnostic antigens, named B13, 1F8 and JL7 recombinant proteins, as potential markers of seroconversion in treated chagasic patients. Prospective study, involving 203 patients treated with benznidazole, was conducted from endemic areas of northern Argentina. Follow-up was possible in 107 out of them and blood samples were taken for serology and PCR assays before and 2, 3, 6, 12, 24 and 36 months after treatment initiation. Reactivity against Trypanosoma cruzi lysate and recombinant antigens was measured by ELISA. The rate of decrease of antibody titers showed nonlinear kinetics with an abrupt drop within the first three months after initiation of treatment for all studied antigens, followed by a plateau displaying a low decay until the end of follow-up. At this point, anti-B13, anti-1F8 and anti-JL7 titers were relatively close to the cut-off line, while anti-T. cruzi antibodies still remained positive. At baseline, 60.8% (45/74) of analysed patients tested positive for parasite DNA by PCR and during the follow-up period in 34 out of 45 positive samples (75.5%) could not be detected T. cruzi DNA. Our results suggest that these antigens might be useful as early markers for monitoring antiparasitic treatment in chronic Chagas disease.

https://doi.org/10.1590/0074-02760160006 1278 downloads

12.

Perfil de expressão do gene fla 14 em tegumento de sementes de soja
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Ramirez Monzón, Daisy

; Borges Bevilacqua, Caroline

; Venske, Eduardo

; Da Maia, Luciano Carlos

; Dejalma Zimmer, Paulo

Investigación Agraria

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dec 2015, Volume 17 N. 2 Pages 108 - 115

Abstract: En Pt Es | Text: En Pt Es | PDF: En | PDF: Pt | PDF: Es

The seed coat has an important role related to seed resistance and deterioration. Therefore, it becomes necessary to understand the mechanisms of genes responsible for traits which can assist developing cultivars with better seed quality. In this sense, the analysis of the expression of fla 14 gene, encoding for the Arabinogalactan protein, which is directly related to cell wall synthesis in soybean seed coat, can contribute to the advancement of this study. Thus, the aim of this work was to validate primers and analyze fla 14 gene expression profile in four soybean genotypes, two of these with black seed coat (TP and IAC 222) and higher lignin content, and two others with yellow seed coat (CD 202 and Potência) and lower lignin content. The plant tissue (seed coat) was collected 50 days after anthesis. qRT-PCR technique was used for genotypes gene expression analysis, through which four combinations of primersconstructed from soybean EST sequence models were tested. The validation results demonstrated the efficiency of FLA 14 p1 and FLA 14 p2 primers in gene expression studies. Regarding fla 14 gene, it was possible to notice that the expression profile of this gene is quantitatively superior in soybean genotypes with black coat and higher lignin content when comparing it with genotypes with yellow coat and lower lignin content.

O tegumento apresenta uma função importante quanto à resistência das sementes e a sua deterioração. Sendo assim, torna-se necessário entender os mecanismos de genes responsáveis por caracteres que possam auxiliar no desenvolvimento de variedades com melhor qualidade. Nesse sentido, a análise da expressão do gene fla 14 que codifica para a proteína Arabinogalactana que esta relacionada diretamente com a síntese da parede celular no tegumento da semente de soja pode contribuir para o avanço dos estudos. Sendo assim, o objetivo do trabalho foi validar primers e analisar o perfil da expressão do gene fla 14 em quatro genótipos de soja, dois desses com tegumento preto (TP e IAC 222) e maior conteúdo de lignina, e outros dois com tegumento amarelo (CD 202 e Potência) e menor conteúdo de lignina. O tecido vegetal (tegumento) foi coletado aos 50 dias após a antese. Para a análise da expressão gênica dos genótipos, utilizou-se a técnica de qRT-PCR, onde foram testadas quatro combinações de primers construídos a partir de modelos de sequência de ESTs da soja. Os resultados da validação demostraram a eficiência dos primers FLA 14 p1 e FLA 14 p2para serem utilizados em estudos de expressão gênica. Em relação ao gene fla 14, pode-se observar queo perfil de expressão deste gene é quantitativamente superior nos genótipos de soja com tegumento preto e conteúdo superior de lignina quando relacionado com os genótipos com tegumento amarelo e menor teor de lignina.

https://doi.org/10.18004/investig.agrar.2015.diciembre.108-115 266 downloads

13.

Análisis morfométrico para la discriminación de especies: el caso del complejo Megalobulimus leucostoma
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Borda, Victor

; Ramirez, Rina

Revista Peruana de Biología

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aug 2014, Volume 21 N. 2 Pages 117 - 124

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Plasticity of conchological characters had led to erroneous descriptions and the accumulation of synonyms making difficult the discrimination among species. The land snail genus Megalobulimus is an example of this problem. Megalobulimus leucostoma (Sowerby, 1835) has three subspecies which are difficult to differentiate by using the original descriptions. The aim of this paper is to discriminate among the subspecies of M. leucostoma by using morphometric and distribution analyses. Both provide substantial differences between M. l. leucostoma and M. l lacunosus that would not support the subspecies status of the former. Megalobulimus leucostoma weyrauchi fits into the great conchological variability of M. l. leucostoma; also the sympatric status between these two subspecies would not support the subspecies status of the former, and M. l. weyrauchi should be considered as part of M. l. leucostoma.

La plasticidad de los caracteres conquiliológicos ha conllevado a diversas descripciones erróneas y a la acumulación de sinonimias lo cual ha dificultado la discriminación entre especies. El género de moluscos terrestres Megalobulimus es un ejemplo de esta problemática. Dentro de éste se encuentra Megalobulimus leucostoma (Sowerby, 1835), que presenta tres subespecies las cuales no se logran diferenciar correctamente a partir de sus descripciones originales. El presente estudio busca discriminar a las subespecies de Megalobulimus leucostoma a partir del análisis morfométrico y de distribución. Ambos análisis establecen diferencias sustanciales entre M. l. leucostoma y M. l. lacunosus que no soportarían el estatus de subespecie de esta última. Megalobulimus leucostoma weyrauchi se ubica dentro de la gran gama de variabilidad conquiliológica de M. l. leucostoma y sus datos de distribución lo ubican en simpatría con la misma por lo cual su estatus como subespecie no estaría soportado, y podría considerarse como parte de M. l. leucostoma.

https://doi.org/10.15381/rpb.v21i2.9814

14.

Ruteo y Asignación de Longitud de Onda: Comparación de Algoritmos Genéticos y Templado Simulado
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Rodriguez, Arturo B

; Gutierrez, Antonio L

; Rivera, Luis A

; Ramirez, Leonardo J

Información tecnológica

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2014, Volume 25 N. 4 Pages 13 - 18

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

In this paper compares studies over routing and wavelength assignment for better some services indicators, such as blocking probability and network utilization. In modern telecommunications are gradually subjected to increased demand, which results in higher requirements in photonic transport networks. This phenomenon forces us improve routing systems, in order to better serve current demand and have capacity of attention of future demand. The comparison is made between the algorithms Genetic Algorithms and Simulated Annealing, both simulated in the optical network NSFNET using wavelength division multiplexing WDM. The results showed that heuristic algorithms are better in dynamic demand and high charge, functioning better under stress. It is concluded that genetic algorithms provide the best solution for future demand.

En este artículo se comparan estudios sobre enrutamiento y asignación de longitud de onda, para mejorar los indicadores de servicio en telecomunicaciones, tales como probabilidad de bloqueo y minimización de la utilización de la red. Las telecomunicaciones modernas están progresivamente sometidas al aumento de la demanda, lo que se traduce en mayores requerimientos en las redes fotónicas de transporte. Este fenómeno obliga a mejorar los sistemas de enrutamiento, con la finalidad de atender mejor a la demanda actual y tener capacidad de atención de la demanda futura. La comparación se realiza entre los algoritmos Templado Simulado (Simulated Annealing) y Algoritmos Genéticos; ambos simulados en la red óptica NSFNET que utiliza multiplexado por división de longitudes de onda, WDM. Los resultados muestran que los algoritmos heurísticos son mejores bajo demanda dinámica de alta carga y que funcionan mejor bajo stress. Se concluye que los algoritmos genéticos dan la mejor solución a la demanda dinámica futura del transporte de datos.

2448 downloads

15.

Total organic carbon removal from a chemical lab's wastewater using Fenton's reagent
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Martínez A, O. M

; Ramirez F, J. H

; Toledo R, M. L

Ingeniería e Investigación

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aug 2013, Volume 33 N. 2 Pages 30 - 35

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

El tratamiento de efluentes residuales industriales representa un serio problema en la actualidad, ya que, por lo general, requiere de soluciones particulares (ad hoc). Este trabajo describe el uso del reactivo Fenton [reacción entre H2O2 y Fe(II)] en la remoción de materia orgánica medida como carbono orgánico total (COT), sobre un efluente proveniente de un laboratorio de análisis químico. Para dicho fin se evaluó el papel que desempeñan algunas de las condiciones de operación del proceso [concentraciones de H2O2 y Fe(II], temperatura y pH] sobre la remoción final de COT del efluente. Al mismo tiempo se desarrolló la optimización económica del tratamiento, ajustando los datos experimentales a un modelo multivariable polinomial de segundo orden que representa el comportamiento del sistema con un R2 de 0.94. Experimentalmente, la mejor remoción encontrada fue de 88,8%, empleando [Fe(II)]0 = 50 mg/L; [H2O2]0 = 50 mM; pH = 2,8 y T = 80 oC, mientras que en el óptimo económico ([Fe(II)]0 = 36 mg/L; [H2O2]0 = 45,5 mM; pH= 2,6 y T = 20 ºC) se alcanzó un 53,9%. Por otra parte, se evidenció que incluso en condiciones adversas para el reactivo Fenton (pH sobre 6) se consiguió una remoción de COT del orden de 41%. Se encontró que la concentración de H2O2 y la temperatura de reacción tienen los efectos más significativos tanto en la eficiencia del proceso como en el costo de operación, lo que las convierte en variables a priorizar en la ejecución del tratamiento.

Treating industrial wastewater represents a serious problem nowadays; it requires a strong understanding of the particular systems and (in most of cases) ad hoc solutions. This work describes the use of Fenton's reagent (reaction between H2O2 and Fe(II)) for removing total organic carbon (TOC) from a particular chemical laboratory's lab-scale batch reactor wastewater. Some operating variables (hydrogen peroxide and ferrous ion concentration, temperature and pH) were evaluated regarding final TOC removal. An economic optimisation was made by means of a second order polynomial model representing these variables' behaviour regarding TOC removal (0.94 R2). The highest experimentally reached TOC removal was 88.8% at 50 mg/L [Fe(II)]0, 50 mM [H2O2]0 , pH=2.8 at 80oC, while 53.9% was obtained in optimised conditions, i.e. 36 mg/L [Fe(II)]0 , 45.5 mM [H2O2]0 , pH=2.6 at 20ºC. It was found that the Fenton process could achieve 41% removal, even in adverse conditions (pH close to 6). It was noted from the analysis that both H2O2 concentration and temperature had a powerful effect on organic matter degradation efficiency, as well as on total treatment cost.

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