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RESUMO OBJETIVO Estimar a soroprevalência de anticorpos do vírus SARS-CoV-2 em escolares de quatro a 14 anos de idade residentes no município de São Paulo, segundo variáveis clínicas, demográficas, epidemiológicas e sociais, durante o período de fechamento das escolas como medida de controle da covid-19. MÉTODOS Realizou-se um inquérito sorológico em setembro de 2020 com amostra aleatória estratificada por tipo de rede de ensino (pública municipal, pública estadual e privada). Foi coletada amostra de sangue venoso utilizando-se o teste de imunoensaio de fluxo lateral da fabricante Wondfo para detecção de anticorpos totais contra o vírus SARS-CoV-2. Aplicaram-se questionários semiestruturados para o levantamento de dados clínicos, demográficos, sociais e epidemiológicos. RESULTADOS A soroprevalência de anticorpos do vírus SARS-CoV-2 em escolares foi de 16,6% (IC95% 15,4–17,8). O estudo encontrou soroprevalências mais elevadas na rede pública municipal (18,5%; IC95% 16,6–20,6) e estadual (16,2%; IC95% 14,4–18,2) em relação à rede privada (11,7; IC95% 10,0–13,7) e entre escolares da raça/cor preta e parda (18,4%; IC95% 16,8–20,2) e no estrato social mais vulnerável (18,5%; IC95% 16,9–20,2). A pesquisa identificou menores soroprevalências nos escolares que relataram seguir as medidas recomendadas de proteção contra a covid-19. CONCLUSÃO A soroprevalência de anticorpos contra o vírus SARS-CoV-2 atinge principalmente os escolares socialmente mais vulneráveis. Este estudo pode contribuir para embasar políticas públicas que reforcem a importância da suspensão das aulas presenciais e da necessidade de estratégias de medidas de proteção e acompanhamento do status sorológico daqueles que ainda não foram contemplados no calendário vacinal.

ABSTRACT OBJECTIVE To estimate seroprevalence of SARS-CoV-2 antibodies in schoolchildren aged 4 to 14 years living in the city of São Paulo, according to clinical, demographic, epidemiological, and social variables, during the school closure period as a measure against covid-19 spread. METHODS A serological survey was made in September 2020 with a random sample stratified by school system (municipal public, state public and private) type. A venous blood sample was collected using the Wondfo SARS-CoV-2 Antibody Test (lateral flow method) for detection of total SARS-CoV-2 virus antibodies. Semi-structured questionnaires were applied to collect clinical, demographic, social, and epidemiological data. RESULTS Seroprevalence of SARS-CoV-2 antibodies in schoolchildren was of 16.6% (95%CI 15.4–17.8). The study found higher seroprevalence in the municipal (18.5%; 95%CI 16.6–20.6) and state (16.2%; 95%CI 14.4–18.2) public school systems compared to the private school system (11.7; 95%CI 10.0–13.7), among black and brown students (18.4%; 95%CI 16.8–20.2) and in the most vulnerable social stratum (18.5 %;95%CI 16.9–20.2). Lower seroprevalence was identified in schoolchildren who reported following the recommended protective measures against covid-19. CONCLUSION Seroprevalence of SARS-CoV-2 antibodies is found mainly in the most socially vulnerable schoolchildren. This study can contribute to support public policies that reinforce the importance of suspending face-to-face classes and developing strategies aimed at protective measures and monitoring of the serological status of those who have not yet been included in the vaccination schedule.

https://doi.org/10.11606/s1518-8787.2023057004782

Seroprevalence of SARS-CoV-2 antibodies in schoolchildren in the city of São Paulo, 2020
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Kamioka, Gabriela Akemi

; Madalosso, Geraldine

; Albuquerque, José Olimpio Moura de

; Costa, Selma Anequini

; Ferreira, Paula Bisordi

; Sato, Ana Paula Sayuri

; Glasser, Paula Regina

; Pino, Francisco Alberto

; Burihan, Patrícia Carla Piragibe Ramos

; Carvalho, Ana Carolina Aguiar de

; Amorim, Ana Beatriz Pagliaro

; Cavazzana, Cinthya Luzia

; Aires, Caroline Cotrim

; Kataoka, Ana Paula Arruda Geraldes

; Savani, Elisa San Martin Mouriz

; Bessa, Thirsa Alvares Franco

; Aguiar, Breno Souza de

; Failla, Marcelo Antunes

; Santos, Edson Aparecido dos

; Brito, Edjane Maria Torreão

; Santos, Maria Cristina Honório dos

; Caldeira, Luiz Artur Vieira

; Silva, Solange Maria Saboia e

; Zamarco, Luiz Carlos

; Fonseca, Sandra Maria Sabino

; Lima, Marcia Maria de Cerqueira

; Marques, Ivanilda Argenau

; Mauro, Athenê Maria de Marco França

; Masi, Eduardo de

Revista de Saúde Pública

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2023, Volume 57 elocation 6s

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

https://doi.org/10.11606/s1518-8787.2023057004782

EFFECT OF A SINGLE RESISTANCE EXERCISE SESSION ON INFLAMMATORY MARKERS AND OXIDATIVE STRESS IN WOMEN LIVING WITH HIV
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Nunhes, Pollyana Mayara

; Marchini, Kauana Borges

; Souza, Dayane Cristina de

; Domingues, Wagner Jorge Ribeiro

; Trindade, Michele Caroline de Costa

; Deminice, Rafael

; Ramos, Solange de Paula

; Avelar, Ademar

Journal of Physical Education

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2023, Volume 34 elocation e3420

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT People living with HIV (PLH), who use antiretroviral therapy (ART), are more susceptible to changes in the inflammatory profile and oxidative stress, and women have greater access to ART. Although physical exercise is a complementary strategy to treatment due to its antioxidant and anti-inflammatory effects, it is not clear whether acute responses to exercise can be harmful to PLH. The aim of the study was to investigate the acute effect of resistance exercise (RE) on inflammatory and oxidative stress markers in PLH. Ten women, using ART, performed RE session consisting of seven exercises for the whole body. For biochemical evaluation, blood samples were collected before (pre), 1 hour (1h) and 2 hours (2h) after the RE session. One-way ANOVA followed by Bonferroni's post hoc test was used to compare results between time points. There was an increase only in markers, GSSG of 160% (pre: 0.40 ± 0.11; 1h: 1.18 ± 0.36; 2h: 1.04 ± 0.25 mmol/g), TNF-α of 98 % (pre: 4.60 ± 0.55; 1h: 6.95 ± 0.77; 2h: 9.10 ± 1.03 pg/ml) and 52% IL-6 (pre: 2.47 ± 0 .67; 1h: 3.63 ± 1.26; 2h: 5.38 ± 2.15 pg/ml). The other variables remained unchanged (P > 0.05). It is concluded that a RE session increased the levels of inflammatory markers and oxidative stress in PLH in a non-exacerbated way.

RESUMO Pessoas vivendo com HIV (PVH), que utilizam a terapia antirretroviral (TARV), são mais suscetíveis a alterações no perfil inflamatório e estresse oxidativo, sendo que as mulheres possuem maior acesso à TARV. Embora o exercício físico seja uma estratégia complementar ao tratamento devido aos seus efeitos antioxidantes e anti-inflamatórios, não está claro se as respostas agudas ao exercício podem ser prejudiciais às PVH. O objetivo do estudo foi investigar o efeito agudo de exercícios com pesos (EP) sobre marcadores inflamatórios e de estresse oxidativo em PVH. Dez mulheres, em uso da TARV, realizaram uma sessão de EP constituída por sete exercícios para o corpo todo. Para avaliação bioquímica, amostras de sangue foram coletadas antes (pré), 1 hora (1h) e 2 horas (2h) após a sessão de EP. A ANOVA one-way seguida do teste post hoc de Bonferroni foi utilizada para comparação dos resultados entre os momentos. Houve aumento apenas nos marcadores, GSSG de 160% (pré: 0,40 ± 0,11; 1h: 1,18 ± 0,36; 2h:1,04 ± 0,25 mmol/g), TNF-α de 98% (pré: 4,60 ± 0,55; 1h: 6,95 ± 0,77; 2h: 9,10 ± 1,03 pg/ml) e IL-6 de 52% (pré: 2,47 ± 0,67; 1h: 3,63 ± 1,26; 2h: 5,38 ± 2,15 pg/ml). As demais variáveis permaneceram sem alterações (P > 0,05). Conclui-se que uma sessão de EP aumentou os níveis de marcadores inflamatórios e estresse oxidativo em PVH de forma não exacerbada.

https://doi.org/10.4025/jphyseduc.v34i1.3420

Clinical performance of resin composite restorations placed by dental students: a retrospective, cross-sectional, and observational study
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Santos, Daiane Cristina Mendonça dos

; Besegato, João Felipe

; Zaniboni, Joissi Ferrari

; Ramos, Solange de Paula

; Cardoso, Sueli de Almeida

; Hoeppner, Márcio Grama

Brazilian Journal of Oral Sciences

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2022, Volume 21 elocation e225991

Abstract: En | Text: En | PDF: En

Aim A retrospective, cross-sectional study was carried out to evaluate the performance of resin composite restorations placed by undergraduate dental students with 1 to 15 years of placement based on dental records. Methods Four calibrated operators evaluated 498 restorations (anterior and posterior) of 120 patients according to Ryge’s validated criteria (USPHS). Results The criteria that showed the smallest changes between the types of failures were color matching, marginal discoloration and surface texture. Regarding the longevity, the surface texture showed an increase in the frequency of failures from the second time interval (3.1 to 6 years). Higher prevalence of failure was found in class II and III restorations, with secondary caries being the main reason. No significant differences were found for anatomic form, marginal adaptation, and color matching. Class V restorations showed a higher fracture rate with total displacement of the restoration, with no increase in the frequency of fracture over time. Conclusion High rate of restoration failure was observed, possibly due to the lack of experience and skills of the students. This outcome highlight the need for continuous revision and improvements of teaching practice regarding the development of clinical competences and skills by dental students.

https://doi.org/10.20396/bjos.v21i00.8665991

Prevalence evolution of SARS-CoV-2 infection in the city of São Paulo, 2020–2021
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Albuquerque, José Olimpio Moura de

; Kamioka, Gabriela Akemi

; Madalosso, Geraldine

; Costa, Selma Anequini

; Ferreira, Paula Bisordi

; Pino, Francisco Alberto

; Sato, Ana Paula Sayuri

; Carvalho, Ana Carolina Aguiar de

; Amorim, Ana Beatriz Pagliaro

; Aires, Caroline Cotrim

; Kataoka, Ana Paula Arruda Geraldes

; Savani, Elisa San Martin Mouriz

; Bessa, Thirsa Alvares Franco

; Aguiar, Breno Souza de

; Failla, Marcelo Antunes

; Santos, Edson Aparecido dos

; Brito, Edjane Maria Torreão

; Santos, Maria Cristina Honório dos

; Silva, Solange Maria Saboia e

; Caldeira, Luiz Artur Vieira

; Zamarco, Luiz Carlos

; Fonseca, Sandra Maria Sabino

; Lima, Marcia Maria de Cerqueira

; Marques, Ivanilda Argenau

; Silva, Fabiana Érica Vilanova da

; Glasser, Paula Regina

; Burihan, Patrícia Carla Piragibe Ramos

; Cavazzana, Cinthya Luzia

; Mello, Debora Silva de

; Pellini, Alessandra Cristina Guedes

; Nishio, Fernando Yoshiki

; Kian, Fernanda Miyashiro

; Braga, Elza de Santana

; Bertelli, Nilza Maria Piassi

; Fracini, Wagner

; Gonçalves, Marcelo Dell Áquila

; Zular, Paulete Secco

; Piva, Regiane de Santana

; Masi, Eduardo

Revista de Saúde Pública

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2021, Volume 55 elocation 62

Abstract: En | Text: En | PDF: En

ABSTRACT OBJECTIVE To estimate the evolution of the prevalence of SARS-CoV-2 virus infection among residents aged 18 years or over in the municipality of São Paulo. METHODS This is a population-based household survey conducted every 15 days, between June and September 2020, and January and February 2021. In total, the study comprised 11 phases. The presence of antibodies against SARS-CoV-2 was identified in venous blood using a lateral flow test, Wondfo Biotech. In the last phase, the researchers combined it with an immunoenzymatic test, Euroimmun. The participants also answered a semi-structured questionnaire on sociodemographic and economic factors, and on social distancing measures. Prevalence estimates and the 95% confidence interval were estimated according to regions, Human Development Index, sex, age group, ethnicity, education, income, and variables associated with risk or prevention of infection. To compare the frequencies among the categories of each variable, the chi-square test with Rao-Scott correction was used, considering a significance level of 5%. RESULTS In total, 23,397 individuals were interviewed and had their samples collected. The estimated prevalence of antibodies against SARS-CoV-2 ranged from 9.7% (95%CI: 7.9–11.8%) to 25.0% (95%CI: 21.7–28.7). The prevalence of individuals with antibodies against the virus was higher among black and brown people, people with lower schooling and income, and among residents of regions with lower Human Development Index. The lowest prevalences were associated with recommended measures of disease protection. The proportion of asymptomatic infection was 45.1%. CONCLUSION The estimated prevalence of the infection was lower than the cumulative incidence variation, except for the last phase of the study. The differences in prevalence estimates observed among subpopulations showed social inequality as a risk of infection. The lower prevalence observed among those who could follow prevention measures reinforce the need to maintain social distancing measures as a way to prevent SARS-CoV-2 infection.

https://doi.org/10.11606/s1518-8787.2021055003970

Prevalence evolution of SARS-CoV-2 infection in the city of São Paulo, 2020–2021
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Albuquerque, José Olimpio Moura de

; Kamioka, Gabriela Akemi

; Madalosso, Geraldine

; Costa, Selma Anequini

; Ferreira, Paula Bisordi

; Pino, Francisco Alberto

; Sato, Ana Paula Sayuri

; Carvalho, Ana Carolina Aguiar de

; Amorim, Ana Beatriz Pagliaro

; Aires, Caroline Cotrim

; Kataoka, Ana Paula Arruda Geraldes

; Savani, Elisa San Martin Mouriz

; Bessa, Thirsa Alvares Franco

; Aguiar, Breno Souza de

; Failla, Marcelo Antunes

; Santos, Edson Aparecido dos

; Brito, Edjane Maria Torreão

; Santos, Maria Cristina Honório dos

; Silva, Solange Maria Saboia e

; Caldeira, Luiz Artur Vieira

; Zamarco, Luiz Carlos

; Fonseca, Sandra Maria Sabino

; Lima, Marcia Maria de Cerqueira

; Marques, Ivanilda Argenau

; Silva, Fabiana Érica Vilanova da

; Glasser, Paula Regina

; Burihan, Patrícia Carla Piragibe Ramos

; Cavazzana, Cinthya Luzia

; Mello, Debora Silva de

; Pellini, Alessandra Cristina Guedes

; Nishio, Fernando Yoshiki

; Kian, Fernanda Miyashiro

; Braga, Elza de Santana

; Bertelli, Nilza Maria Piassi

; Fracini, Wagner

; Gonçalves, Marcelo Dell Áquila

; Zular, Paulete Secco

; Piva, Regiane de Santana

; Masi, Eduardo

Revista de Saúde Pública

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2021, Volume 55 elocation 62

Abstract: En | Text: En | PDF: En

https://doi.org/10.11606/s1518-8787.2021055003970 43 downloads

Clinical and radiographic evaluation of a new stain-free tricalcium silicate cement in pulpotomies
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LIMA, Suéllen Priscilla Rodrigues de

; SANTOS, Greissy Lopes dos

; FERELLE, Antonio

; RAMOS, Solange de Paula

; PESSAN, Juliano Pelim

; DEZAN-GARBELINI, Cássia Cilene

Brazilian Oral Research

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2020, Volume 34 elocation e102

Abstract: En | Text: En | PDF: En

Abstract The aim of this study was to evaluate and compare the clinical and radiographic success rates of pulpotomies in primary molars using a new stain-free tricalcium silicate cement (Bio-C Pulpo) versus a conventional white MTA (MTA Angelus) as a pulpotomy medicament for primary teeth. Seventy primary teeth in 33 patients were randomly divided into two groups, MTA Angelus (n = 34) and Bio-C Pulpo (n = 36). Clinical and radiographic follow-up examinations were conducted at seven days (except radiographic follow-up), and at one, three, six and 12 months. At 12 months, the clinical success rate of the MTA Angelus group was 100 percent (28 out of 28), and the radiographic success rate was 100 percent (28 out of 28). In comparison, the clinical success rate for the Bio-C Pulpo group was 100 percent (29 out of 29), and the radiographic success rate was 100 percent (29 out of 29). No significant differences were found between the groups at any follow-up evaluation. According to Fisher´s exact test, a significant difference was observed between the pulpotomy agent and tooth discoloration (p < 0.001). The success rates were 100% for the Bio-C Pulpo group, with no case of discoloration, and 10.31% for the MTA Angelus group. Both MTA Angelus and Bio-C Pulp pulpotomy medicaments are appropriate options for pulpotomies in primary teeth, and enable high clinical and radiographic success rates. However, more long-term studies are required to test the new Bio-C Pulpo medicament.

https://doi.org/10.1590/1807-3107bor-2020.vol34.0102 877 downloads

Training aimed at the development of power and physical performance of futsal players
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Freitas, Victor Hugo de

; Rinaldo, Márcio

; Turquino, Guilherme Guimarães

; Miloski, Bernardo

; Ramos, Solange de Paula

Revista Brasileira de Cineantropometria & Desempenho Humano

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2019, Volume 21 elocation e60119

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O objetivo do estudo foi analisar o efeito de um período de treinamento direcionado ao desenvolvimento de potência de membros inferiores no desempenho físico de jogadores de futsal. A amostra foi composta por 10 jogadores de futsal de uma equipe profissional com 23,70 ± 5,85 anos,72,33 ± 6,01 kg e 174,40 ± 0,05 cm. Os jogadores foram monitorados por um período de 4 semanas de treinamento, correspondente ao início do período competitivo. Neste período, o treinamento físico buscouaperfeiçoar a potência de membros inferiores. No início e ao término do período, foram realizados os testes: Squat jump (SJ), Countermovemt jump (CMJ), velocidade de 15 metros (Vel_15m) e Futsal Intermitente Endurance Test (FIET). A carga de treinamento foi monitorada pelo método da percepçãosubjetiva de esforço da sessão. As semanas 1 e 3 apresentaram maior CTST que as semanas 2 e 4 (p < 0,01). No momento pós treinamento, observou-se menor tempo de corrida no teste de Vel_15m (p = 0,04), maior altura nos testes SJ (p < 0,01) e CMJ (p < 0,01), e maior velocidade no FIET (p = 0,04). Pode-se concluir que direcionar o treinamento no início da temporada competitiva para melhorada potência proporciona incremento na potência de membros inferiores de jogadores de futsal, com consequente melhora da velocidade e na capacidade de realizar ações intermitentes em alta intensidade.

Abstract The aim of the study was to analyze the effect of a training period aimed at the development of lower limb power and physical performance of futsal players. The sample consisted of 10 futsal players from a professional team aged 23.70 ± 5.85 years, 72.33 ± 6.01 kg and 174.40 ± 0.05 cm. Players were monitored for a period of 4 training weeks, corresponding to the beginning of the competitive period. During this period, physical training sought to improve lower limb power. At the beginning and end of the training period, the following tests were performed: Squat jump (SJ), Countermovement jump (CMJ), 15-meter speed(Vel_15m) and Futsal Intermittent Endurance Test (FIET).The training load was monitored by the Session Perceived Exertion method.Weeks 1 and 3 presented higher training load than weeks 2 and 4 (p <0.01). In the post-training period, shorter running time in the Vel_15m test (p = 0.04), greater height in SJ (p <0.01) and CMJ (p <0.01) tests, and higher maximal speed in FIET (p = 0.04) were observed. It could be concluded that directing training at the beginning of the competitive season to improve power increases the lower limb power of futsal players, with consequent improvement of speed and the ability to perform intermittent actions at high intensities.

https://doi.org/10.1590/1980-0037.2019v21e60119 1837 downloads

Efeitos do metilfenidato sobre as glândulas salivares maternas de camundongos
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LIMA, Karol Sartori

; SALLES, Antonio Eduardo

; COSTA, Gabriel Araujo

; RAMOS, Solange Paula

; SALLES, Maria José Sparça

Revista de Odontologia da UNESP

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dec 2016, Volume 45 N. 6 Pages 316 - 321

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Introdução O metilfenidato (MFD) é um derivado anfetamínico estimulante do sistema nervoso central, que vem sendo cada vez mais consumido pela população mundial, incluindo as mulheres em idade fértil. Ainda não foram estabelecidos os efeitos deste medicamento nas glândulas salivares, durante a gestação. Objetivo Identificar alterações histomorfológicas em glândulas salivares maternas expostas ao metilfenidato. Material e método Para análise histológica, foram utilizadas 32 fêmeas de camundongos Swiss prenhes, distribuídas em um grupo controle e um grupo tratado. A administração foi realizada do quinto ao 17º dia de gestação, via injeção subcutânea; o grupo tratado recebeu 5 mg/kg de metilfenidato, enquanto o grupo controle recebeu o mesmo volume de solução salina estéril. As fêmeas foram eutanasiadas e tiveram suas glândulas salivares removidas e incluídas em parafina para análise em microscopia óptica. Para avaliar a associação entre as variáveis dos grupos, o teste de Kolmogorov-Smirnov e o teste T foram utilizados. Resultado As glândulas parótidas do grupo tratado apresentaram alterações no raio dos ductos e na quantidade de ácinos, quando comparadas às glândulas parótidas do grupo controle. A glândula submandibular do grupo tratado foi a mais afetada: mostrou diferença estatisticamente significativa na espessura da parede dos ductos secretores, no raio dos ductos e no raio dos ácinos, quando comparada à glândula submandibular do grupo controle. A glândula sublingual não apresentou alterações significativas. Conclusão Neste delineamento experimental, o metilfenidato apresentou-se como agente indutor de alterações morfológicas das glândulas salivares, promovendo alterações significativas nos raios de ductos e ácinos das mesmas, sendo a glândula submandibular a mais susceptível a este fármaco.

Abstract Introduction Methylphenidate (MFD) is a stimulant amphetamine derivative of the central nervous system, which is being increasingly consumed by the world population, including women of childbearing age. It has not yet established the effects of this medicine in the salivary glands during pregnancy. Objective Evaluate the exposure effects to the MPD in the maternal salivary glands. Material and method For prenatal study, 32 pregnant female Swiss mice were used, divided into a control and a treated group. The treated group received 5 mg/kg of MPD via subcutaneous injection from the 5th to the 17th day and the control received sterile saline in the same volume and pregnancy period. Females were euthanized and had their salivary glands removed and embedded in paraffin for analysis in optical microscopy. To evaluate the association between the variables of the groups, the Kolmogorov-Smirnov test and and T test were used. Result The parotid glands of the treated group showed statistically significant change within the ducts and the amount of acini when compared to the control group. The submandibular gland was the most affected, it showed a difference statistically significant in wall thickness of the secretory ducts, within the ducts and within the acini when compared to the control group. The sublingual gland showed no significant changes. Conclusion In this experiment methylphenidate is presented as inducing agent morphological changes of the salivary glands, promoting significant changes in ducts radii and acini, submandibular gland being more susceptible to this drug.

https://doi.org/10.1590/1807-2577.19415 1770 downloads

10.

Anti-dentine Salivary SIgA in young adults with a history of dental trauma in deciduous teeth
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SEIXAS, Gabriela Fleury

; GARBELINI, Cássia Cilene Dezan

; AIDA, Kelly Limi

; CHEIRUBIM, Ana Paula

; VENÂNCIO, Emerson José

; RAMOS, Solange de Paula

Brazilian Oral Research

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2015, Volume 29 N. 1 elocation 00298

Abstract: En | Text: En | PDF: En

Anti-dentin autoantibodies are associated with inflammatory root resorption in permanent teeth and are modulated by dental trauma and orthodontic force. However, it is not known whether deciduous tooth trauma can stimulate the development of a humoral immune response against dentin. The aim of this study was to evaluate the levels of salivary SIgA reactivity against human dentin extract in young adults with a history of trauma in the primary dentition. A sample of 78 patients, aged 18 to 25, who had completed an early childhood (0 to 5 years old) caries prevention program years earlier at the Universidade Estadual de LondrinaPediatric Clinic, underwent radiographic examination and salivary sampling. Anti-dentin SIgA levels were analyzed by immunoenzymatic assay and Western blotting. Although dental trauma to deciduous teeth had occurred in 34 (43.6%) of the patients, no differences in SIgA levels were detected between individuals who had experienced trauma and those who had not (p > 0.05). Multivariate regression analysis showed no association between dental trauma and SIgA levels (p > 0.05). Patients with a history of deciduous trauma presented low levels of anti-dentin antibodies, associated with orthodontic root resorption (p < 0.05). Western blot analysis showed that salivary antibodies recognized a single band of approximately 45 kDa in dentin extract. We concluded that salivary SIgA recognizes a specific component of the dentin matrix and that anti-dentin antibodies were not triggered by trauma to primary teeth. However, trauma to deciduous teeth may down-modulate SIgA in response to orthodontic root response.

https://doi.org/10.1590/1807-3107BOR-2015.vol29.0102 1368 downloads

11.

Caffeine and physical training: effects on cardiac morphology and cardiovascular response
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Ruiz, Roberto

; Ramos, Solange de Paula

; Pinge, Marli Martins

; Moraes, Solange Franzói de

; Polito, Marcos

Revista da Associação Médica Brasileira

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feb 2014, Volume 60 N. 1 Pages 23 - 28

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Objetivo analisar a histologia cardíaca, a pressão arterial (PA), a frequência cardíaca (FC) e a variabilidade da frequência cardíaca (VFC) de ratos submetidos a treinamento físico e suplementação de cafeína. Métodos sessenta ratos foram divididos em grupos controle (CO), controle suplementado com cafeína (CAF), treinados controle (TRE) e treinados suplementados com cafeína (TCAF). Os grupos de treinamento realizaram natação por quatro semanas, e os grupos de cafeína foram suplementados por ingestão voluntária de cafeína diluída em água. Resultados não houve modificações para PA e FC entre os grupos. Em relação à VFC, houve diminuição nos componentes LFnorm (baixa frequência) e LF/HF (razão baixa e alta frequência) em TCAF em relação a CAF (p<0,02 e p<0,03, respectivamente). Houve também aumento em CAF em relação a CO no componente LFnorm (p<0,05). Os resultados também mostraram aumento no peso relativo do coração em TRE (p<0,04) e TCAF (p<0,03) em relação a CO. Conclusão a cafeína não modificou as respostas hemodinâmicas. Entretanto, a natação diminuiu a resposta simpática e aumentou o peso relativo do coração.

Objective to analyze the morphological structure of cardiac, blood pressure (BP), heart rate (HR) and heart rate variability (HRV) of rats subjected to physical training with supplementation of caffeine. Methods 60 rats were divided into 4 groups: control (CO), control with caffeine (CAF), trained control (TRE) and trained with caffeine (TCAF). All trained groups underwent 4 weeks of swimming, and all caffeine groups were supplemented by voluntary ingestion of caffeine diluted in drinking water. Results there were no changes to BP and HR between groups. Regarding HRV, there was a decrease in LFnorm (low frequency) and LF/HF ratio (low and high frequency) in TCAF and CAF compared to group (p<0.02 and p<0.03, respectively). An increase occurred in CAF compared to the CO in the component LFnorm (p<0.05). The results also showed an increase in the relative weight of heart in the TRE (p<0.04) and TCAF (p<0.03) compared to CO. Conclusion caffeine did not modify the hemodynamic responses. However, physical training resulted in a decrease in sympathetic response and an increase in relative heart weight.

https://doi.org/10.1590/1806-9282.60.01.007 6480 downloads

12.

Efeitos comportamentais e imunológicos da fluoxetina em ratos submetidos ao nado forçado
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Fernandes, Eduardo Vignoto

; Ramos, Solange de Paula

; Estanislau, Celio

; Venancio, Emerson José

Psicologia: Teoria e Pesquisa

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dec 2012, Volume 28 N. 4 Pages 409 - 415

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

No presente trabalho, os efeitos comportamentais e imunomoduladores da fluoxetina foram avaliados num modelo animal de depressão, o nado forçado. Nesse, o comportamento de flutuar é sensível a antidepressivos e é usado como índice de desespero comportamental. Foram utilizados dois grupos experimentais, sendo um grupo controle tratado com salina e outro grupo teste tratado com a fluoxetina, ambos administrados por via intra-peritoneal. Os animais foram tratados três vezes ao dia por 12 dias. Adicionalmente, a resposta imune humoral a uma imunoestimulação (hemácias de carneiro) foi avaliada. A fluoxetina provocou aumento no tempo em flutuação, queda na massa corporal e menor produção total de anticorpos. Os resultados indicam uma modulação simultânea do comportamento e do sistema imunológico pela fluoxetina.

In the present study, behavioral and immunomodulatory effects of fluoxetine were evaluated with the forced swim test, an animal model of depression. In this model, floating behavior is responsive to antidepressants and is used as a behavioral index of despair. During 12 days, three times a day, two groups were treated intraperitoneally with saline or fluoxetine. Additionally, the humoral immune response to immunostimulation (sheep red blood cells) was evaluated. The animals treated with fluoxetine floated longer, showed decreases in body mass and had lower total production of antibodies. The results indicate simultaneous modulation of behavior and antibody production by fluoxetine.

12852 downloads

13.

Efeito do treinamento físico e da ingestão crônica de cafeína sobre o tecido ósseo de ratos jovens
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Ramos, Solange de Paula

; Santos, Vanessa Batista da Costa

; Ruiz, Roberto José

; Preti, Maria Carla Perozim

; Ventura, Mayara Caroline

; Nogueira, Maiara Santos

; Campana, Francelaine Bruna

; Müller, Sérgio Swain

; Polito, Marcos Doederlein

; Siqueira, Claudia Patrícia Cardoso Martins

Revista Brasileira de Educação Física e Esporte

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jun 2012, Volume 26 N. 2 Pages 209 - 217

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Foram avaliados os efeitos da ingestão crônica de cafeína e exercício de natação sobre o tecido ósseo. Ratos Wistar jovens (n = 32) alocados em quatro grupos: sedentário controle (SCO), sedentário + cafeína (SCAF), treinados (TCO) e treinados + cafeína (TCAF), receberam 1 mg/ml de cafeína em água durante 30 dias (SCAF e TCAF) e foram submetidos a 21 dias de natação em intensidade moderada (TCO e TCAF). A tíbia esquerda foi submetida a ensaio biomecânico para determinação do coeficiente de rigidez, limite de elasticidade e carga máxima. A área de tecido ósseo primário e espessura do disco epifisário foram avaliadas no fêmur. Não foram observadas alterações nas propriedades biomecânicas e na deposição de tecido ósseo primário. O grupo SCAF apresentou maior espessura no disco epifisário distal. Os resultados sugerem que o treinamento moderado de natação e a ingestão de cafeína não provocaram alterações morfológicas e biomecânicas do tecido ósseo.

Effects of swimming training and chronic caffeine intake on bone tissue of young rats were investigated. Young Wistar rats were allocated into 4 groups: sedentary control (SCO), sedentary + caffeine (SCAF), trained (TCO) and trained + caffeine (TCAF); they received 1 mg/ml of caffeine diluted in water for 30 days (SCAF and TCAF) and were submitted to swimming training of moderate intensity for 21 days (TCO and TCAF). The left tibia was subjected to the biomechanical test for determination of the coefficient of stiffness, yield and maximum load. The immature bone tissue area and thickness of growth plates were evaluated in the femur. No changes were observed in biomechanical properties and the area occupied by immature bone tissue. However, SCAF group presented greater thickness in distal growth plate. The results suggest that the moderate intensity swimming training and caffeine consumption were not able to promote changes in morphology and biomechanical properties of bone tissue.

Se evaluaram los efectos del consumo crónico de cafeína y la natación em el hueso. Ratas Wistar jóvenes (n=32) divididas en cuatro grupos: sedentarios control (SCO), sedentarios + cafeína (SCAF), entrenados (TCO) y entrenados + cafeína (TCAF), recibieron 1 mg/ml de cafeína en agua durante 30 días (SCAF e TCAF) e fueron sometidos 21 días de la natación moderada. La tibia izquierda se sometió a una prueba biomecánica para determinar la coeficiente de la rigidez, el limite de elasticidad y el punto de carga máxima. El area de hueso imaturo e de la zona de la placa de crescimiento proximal e distal se analizó em el fêmur. No se observaron cambios en las propiedades biomecánicas y el déposition de hueso inmaturo. Animales SCAF tenía más grueso de la placa de crescimiento distal. Los resultados sugieren que entrenamento moderalado de la natación y el cosumo crônico de cafeína no cambió la morfología y las propiedades biomecánicas del hueso.

5495 downloads Cited 2 times in SciELO

14.

Comparison of thermotolerant coliforms and Escherichia coli densities in freshwater bodies
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Hachich, Elayse M.

; Di Bari, Marisa

; Christ, Ana Paula G.

; Lamparelli, Cláudia C.

; Ramos, Solange S.

; Sato, Maria Inês Z.

Brazilian Journal of Microbiology

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jun 2012, Volume 43 N. 2 Pages 675 - 681

Abstract: En | Text: En | PDF: En

Fecal bacterial indicator analyses have been widely used for monitoring the water quality. This study was designed to determine the ratio between the density of Escherichia coli and other Thermotolerant Coliforms (TtC) bacteria from freshwater samples collected for a two-year period of monitoring. TtC were enumerated by membrane filtration on mFC agar. E. coli enumeration was done by two methods: TtC colonies identified in mFC were inoculated in EC-MUG or water samples were filtered and inoculated in modified mTEC agar media, and both methods were compared for quantitative recovery of E. coli. The results pointed out a mean percentage of E. coli among other thermotolerant coliforms (E. coli/TtC ratio) of 84.3% in mFC media. Taking these results into account, a mandatory standard of 1000 thermotolerant coliforms would correspond to 800 E. coli and the adoption of these E. coli based standards will represent a major improvement for the monitoring of freshwater quality.

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15.

Relação entre métodos de quantificação de cargas de treinamento baseados em percepção de esforço e frequência cardíaca em jogadores jovens de futsal
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Milanez, Vinicius Flávio

; Ramos, Solange de Paula

; Salle-Neto, Fuad

; Machado, Fabiana Andrade

; Nakamura, Fábio Yuzo

Revista Brasileira de Educação Física e Esporte

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mar 2012, Volume 26 N. 1 Pages 17 - 27

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Os objetivos do estudo foram verificar a associação entre os métodos de quantificação da carga de treinamento, PSE da sessão e impulso de treinamento proposto por Lucía (LuciaTRIMP) e descrever a distribuição da intensidade de treinamento no futsal semiprofissional da categoria sub 18. Quinze jogadores (média e DP; idade: 17 ± 1 ano; massa corporal: 70,4 ± 6,5 kg; estatura: 175 ± 4 cm e IMC 23 ± 2 kg.m-2) foram submetidos a um teste de corrida incremental para determinar o limiar ventilatório (LVmax), o ponto de compensação respiratória (PCR) e o consumo máximo de oxigênio (VO2max). Apenas oito atletas foram monitorados por meio dos métodos PSE da sessão e LuciaTRIMP durante 78 sessões de treinamento antes da principal competição da temporada. Foram encontradas correlações individuais de magnitudes alta a quase perfeita (r = 0,64 - 0,91) entre os métodos PSE da sessão e LuciaTRIMP. O padrão de distribuição da intensidade de treinamento foi 76, 18 e 6% do tempo abaixo do LV (zona 1), entre LV e PCR (zona 2) e acima do PCR (zona 3), respectivamente. O método PSE da sessão se mostrou altamente relacionado com um método objetivo de quantificação da carga de treinamento. Além disso, as sessões de treinamento de uma equipe de futsal semiprofissional da categoria sub 18 apresentaram um padrão decrescente de distribuição das intensidades de treinamento.

The aims of this study were to verify the association between the methods of training load quantification session-RPE and training impulse -proposed by Lucia (LuciaTRIMP) -and to describe the training intensity distribution in under-18 semi-professional futsal. Fifteen male players (mean and SD; age: 17 ± 1 years; body mass: 70.4 ± 6.5 kg; height: 175 ± 4 cm and BMI 23 ± 2 kg.m²) performed an incremental treadmill test to determine the ventilatory threshold (VT), respiratory compensation point (RCP), and maximal oxygen consumption (VO2max). Only eight athletes had their training loads quantified by means of session-RPE and Lucia methods over a total of 78 training sessions before the main competition of the season. High to almost perfect correlations (r = 0.64 - 0.91) were found between session-RPE and LuciaTRIMP methods. The distribution of the training intensity was, respectively, 76, 18 e 6% of training duration below VT (zone 1), between VT and RCP (zone 2), and above RCP (zone 3). The session-RPE method was highly associated with an objective method of training load quantification. Furthermore, the training sessions of a semi-professional under-18 futsal team showed a decreasing training intensity distribution.

11429 downloads Cited 1 time in SciELO

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