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Abstract Introduction: In Mexico, cardiac rehabilitation (CR) as an interdisciplinary intervention with therapeutic impact in patients with heart disease is growing. There is the need to know actual conditions of CR in our country. Objectives: The objective of this National Registry is to follow-up those existing and new CR units in Mexico through the comparison between the two previous registries, RENAPREC-2009 and RENAPREC II-2015 studies. This is a descriptive study focused on diverse CR activities such as assistance training, and certification of health professionals, barriers, reference, population attended, interdisciplinarity, permanence over time, growth prospects, regulations, post-pandemic condition, integrative characteristics, and scientific research. Results: Data were collected from 45 CR centers in the 32 states, 75.5% are private practice units, 67% are new, 33% were part of RENAPREC II-2015, and 17 have continued since 2009. With a better distribution of CR units along the territory, the median reference of candidates for CR programs is 9% with a significant reduction into tiempo of enrollment to Phase II admission (19 ± 11 days). Regarding to previous registries, the coverance of Phases I, II, and III is 71%, 100%, and 93%, respectively; and a coverance increases in evaluation, risk stratification, and prescription, more comprehensive attendance and prevention strategies. Conclusions: CR in Mexico has grown in the past 7 years. Even there is still low reference and heterogeneity in specific processes, there are strengths such as interdisciplinarity, scientific professionalization of specialists, national diversification, and an official society that are consolidated over time.

https://doi.org/10.24875/acm.22000235

Principios y prácticas agroecológicas para la transición hacia una ganadería bovina sostenible. Revisión
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Angel-Lozano, Guadalupe Del

; Escalona-Aguilar, Miguel Ángel

; Baca del Moral, Julio

; Cuevas-Reyes, Venancio

Revista mexicana de ciencias pecuarias

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sep 2023, Volume 14 N. 3 Pages 696 - 724

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen El desarrollo del sector ganadero bovino es afectado por múltiples crisis como la ambiental, económica, alimentaria, energética, sanitaria y migratoria, que impactan en la alimentación y la salud de las personas. Estas crisis obligan a las y los productores a reflexionar y repensar sobre las prácticas ganaderas que aplican en el contexto sociocultural, ambiental, económico y político. La ganadería bovina sostenible, permite enfrentar estas crisis mediante la aplicación de prácticas agroecológicas. El objetivo de esta revisión es analizar los principios y prácticas agroecológicas que promueven una transición hacia la sostenibilidad del sector ganadero bovino, a través de un análisis bibliográfico se discutió el impacto de la crisis civilizatoria en la ganadería bovina, así como las perspectivas de la agroecología y sus alcances, esto con la finalidad de identificar sus contribuciones para alcanzar una producción de leche y carne accesible y de calidad para la sociedad. Se concluye que los principios y prácticas agroecológicas son universales y pueden ser aplicados a la ganadería bovina para transitar a sistemas de producción sustentable. Estos principios y prácticas pueden adaptarse a zonas climáticas y reducir el impacto que la crisis civilizatoria ha generado en la ganadería bovina. La aplicación de dichos principios y prácticas debe ser de acuerdo al nivel, cantidad y calidad de los recursos de cada dimensión. Las iniciativas analizadas muestran que los niveles de producción de carne y leche son llevados a cabo con el menor número de insumos posible, un bajo impacto al medio ambiente y con la formación de comunidades organizadas. Finalmente, su aplicación apropiada depende en mayor medida de la disposición, motivación y el empoderamiento de las y los productores.

Abstract The development of the livestock sector is affected by environmental, economic, food, energy, sanitary, and migratory crises, all of which have an impact on people's food and health. These crises force producers to reflect and rethink the livestock breeding practices they apply within the sociocultural, environmental, economic, and political context. Sustainable cattle ranching makes it possible to face these crises by applying agroecological practice. The objective of this review is to analyze the agroecological principles and practices that promote a transition toward sustainability in the cattle sector. Both the impact of the civilization crisis on cattle raising and the perspectives and scope of agroecology were discussed with the aim of identifying the contributions of this discipline to the production of good-quality milk and meat accessible to society. It was concluded that agroecological principles and practices are universal and can be applied to cattle ranching in order to achieve sustainable production systems. These principles and practices can be adapted to climate zones and reduce the impact that the civilization crisis has generated in cattle raising. These principles and practices should be applied according to the level, quantity, and quality of the resources of each dimension. The analyzed initiatives show that meat and milk are produced with the lowest possible inputs, a low environmental impact, and the formation of organized communities. Finally, their proper application depends to a large extent on the willingness, motivation, and empowerment of the producers.

https://doi.org/10.22319/rmcp.v14i3.6287

Online Training for Primary Care Staff: Skills development on the Mental Health Gap Action Programme (mhGAP) Intervention Guide
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Félix Romero, Violeta

; Tovar, Ángel Eugenio

; López Montoya, Alejandra

; Flores Coronado, Marco Antonio

; Ferrer Reyes, Sandra Ivonne

; Morales Chainé, Silvia

; Malo Serrano, Miguel

Acta de investigación psicológica

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aug 2023, Volume 13 N. 2 Pages 88 - 99

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Developing effective learning strategies to strengthen mental health professionals’ capacities and deliver evidence-based interventions in their communities is urgent. We developed and evaluated an online training program for the Intervention Guide for Mental, Neurological and Substance Use Disorders in Non-specialized Health Settings. Nine hundred and seventy-five health professionals in Mexico were enrolled in the training program, during the period of social distancing brought about by the COVID-19 pandemic. Participants completed a pre-post online evaluation strategy including Knowledge screening, assessment of Learning Activities, and performance in Programmed-Simulated cases to evaluate knowledge and skills for the assessment, management, and follow-up of Mental, Neurological and Substance Use Disorders. We found that participants improved their knowledge and skills from training on the mhGAP online course. Notably we observed these positive results regardless of sex, profession, institution, or social vulnerability rating of participants, suggesting that this is a relevant training program for primary care staff. These results contribute to the Mental Health Gap Action Programme and advance the use of online teaching and evaluation technologies in this field.

Resumen El desarrollo de estrategias efectivas de aprendizaje para fortalecer las competencias de los profesionales de la salud mental y brindar intervenciones basadas en evidencia en sus comunidades es necesario. El objetivo del presente trabajo fue desarrollar y evaluar un programa de entrenamiento en línea para la Guía de Intervención en Trastornos Mentales, Neurológicos y por Uso de Sustancias en nivel de atención de salud no especializada. Participaron 975 profesionales de la salud mexicanos durante el período de distanciamiento social provocado por la pandemia de COVID-19. Los participantes completaron una evaluación previa y posterior que incluyó un cuestionario de conocimientos, actividades de aprendizaje y la ejecución en casos simulados programados para evaluar el conocimiento y las habilidades para la evaluación, el manejo y el seguimiento de los trastornos mentales, neurológicos y por uso de sustancias. Los resultados indicaron que los participantes mejoraron sus conocimientos y habilidades en función de su participación en el curso en línea, independientemente del sexo, la profesión, la institución o la vulnerabilidad social de los participantes, sugiriendo que se trata de un programa de formación relevante para el personal de atención primaria. Los resultados contribuyen al Programa de Acción para la Brecha de Salud Mental y promueven el uso de tecnologías de evaluación y enseñanza en línea en este campo.

https://doi.org/10.22201/fpsi.20074719e.2023.2.486

Introgression of transgenic events and accompanying sequences into Mexican maize varieties
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Siller-García, Jessica Nallely

; Cruz-González, Miguel Ángel

; Castillo-Reyes, Francisco

; Rodríguez-Herrera, Sergio Alfredo

; Morlett-Chávez, Jesús Antonio

; Aguilar, Cristóbal N.

; Rodríguez-Herrera, Raúl

Ecosistemas y recursos agropecuarios

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aug 2023, Volume 10 N. 2 elocation e3538

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen: Se recolectaron muestras de semillas de 215 de variedades de maíz representativas de cuatro (Jalisco, Michoacán, Oaxaca y Puebla) estados de México. El total de genotipos colectados en cada Estado fue de 53, 96, 46 y 20, respectivamente. Cada colecta de semilla fue georreferenciada; posteriormente cada colecta se sembró y el tejido foliar se usó para el aislamiento de ADN, luego se realizó un análisis de PCR para determinar la presencia o ausencia de ocho secuencias acompañantes y nueve eventos transgénicos en los 215 genotipos. Los resultados mostraron la introgresión de secuencias acompañantes y eventos transgénicos (cry1Ab) en el genoma de algunas variedades locales de maíz. La mayor proporción de residuos de cry1Ab observados en las variedades locales de maíz fue la muestra de Puebla (70%), seguida por las muestras de Oaxaca donde se observó 52%, mientras que 15 y 7.5% de los maíces nativos del estado de Jalisco presentaron el gen cry1Ab y la secuencia acompañante ntpII, respectivamente. Las muestras de Michoacán mostraron 44 y 17.7% para el gen cry1Ab y la secuencia acompañante, respectivamente. Estos resultados deben respaldar la aplicación de medidas correctivas para prevenir la contaminación genética de variedades locales de maíz y limpiar fenotipos fuera de tipo que podrían ser fuentes de contaminación de genes transgénicos dentro de poblaciones de variedades locales de maíz contaminadas con estos transgenes.

Abstract Representative seed samples of 215 of maize varieties from four (Jalisco, Michoacán, Oaxaca and Puebla) Mexican States were collected. The total genotypes collected in each State were 53, 96, 46 and 20 samples, respectively. Each seed collection was geo-referenced, then seed was planted and leaf tissue was used for DNA isolation, after that a PCR analysis was performed to determine the presence or absence of eight accompanying sequences and nine transgenic events in the 215 maize genotypes. Results showed introgression of accompanying sequences and transgenic events (cry1Ab) into some maize varieties. The major proportion of cry1Ab residues was observed in maize samples from Puebla (70%) followed by that displayed in the Oaxaca samples (52%), while Jalisco samples showed 15 and 7.5% of the cry1Ab gene and ntpII accompanying sequence, respectively, and Michoacán samples exhibited 44 and 17.7% for cry1Ab gene and the accompanying sequence, respectively. These results supported for applicate corrective measures for to prevent the genetic contamination of Mexican maize varieties and to clean up out-of-type phenotypes that could be sources of contamination of transgenic genes within maize populations contaminated with transgenes.

https://doi.org/10.19136/era.a10n2.3538

Beyond the hammer: reflections on neurology residency in Mexico
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Medina-Rioja, Raul

; Saldivar-Davila, Sergio

; Reyes-Cortes, Idarmis

; García-Grimshaw, Miguel

Revista mexicana de neurociencia

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aug 2023, Volume 24 N. 4 Pages 99 - 101

Abstract: En | Text: En | PDF: En

https://doi.org/10.24875/rmn.23000029

Algunas reflexiones sobre la actualización de los médicos
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Ruiz-Argüelles, Guillermo J.

; Rocha-Arrieta, Luisa L.

; Arrieta, Oscar

; Celis, Miguel A.

; Domínguez-Cherit, Judith

; Islas-Andrade, Sergio

; Lifshitz, Alberto

; Mansilla-Olivares, Armando

; Martínez, Iris

; Mimenza, Alberto José

; Moreno, Mucio

; Reyes-Sánchez, Alejandro A.

; Soda-Merhy, Antonio

; Sotelo, Julio

; Toussaint, Sonia

; Vilar-Compte, Diana

; Verástegui, Emma

Gaceta médica de México

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aug 2023, Volume 159 N. 4 Pages 284 - 285

Abstract: Es | Text: Es | PDF: Es

https://doi.org/10.24875/gmm.23000137

Guía de práctica clínica para el manejo del cáncer de pulmón de células pequeñas: enfermedad extensa
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Cabrera-Miranda, Luis A.

; Díaz-García, Diego A.

; Corona-Cruz, José F.

; Lozano-Ruiz, Francisco J.

; Sánchez-Reyes, Roberto

; Álvarez-Bojórquez, Mario E.

; Blake-Cerda, Mónika

; Rivera-Márquez, Raúl

; López-Saucedo, Raúl A.

; Pérez-Álvarez, Sandra I.

; Bolaño-Guerra, Laura M.

; Alatorre-Alexander, Jorge A.

; Alexander-Meza, Francisco

; Barrón-Barrón, Feliciano

; Blanco-Vázquez, Yazmín C.

; Campos-Gómez, Saúl

; Mata-Moya, Dolores de la

; Figueroa-Martínez, Pedro

; González-Cisneros, Paulina E.

; Íñiguez-García, Marco A.

; Lázaro-León, Jesús M.

; Loyola-García, Ulises

; Morales-Rivera, Marcelino

; Olivares-Torres, Carlos

; Ramos-Ramírez, Maritza

; Sáenz-Frías, Julia A.

; Silva-Bravo, Fernando

; Trejo-Rosales, Rogelio

; Souto-del Bosque, Miguel

; Arrieta, Óscar

Gaceta mexicana de oncología

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jun 2023, Volume 22 N. 2 Pages 55 - 73

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Antecedentes: El cáncer de células pequeñas (CPCP) representa el 13-15% del total de neoplasias primarias de pulmón. Se caracteriza por su rapidez en la tasa de crecimiento y en el desarrollo de metástasis a distancia. Objetivos: Orientar y estandarizar el tratamiento del CPCP enfermedad extensa en México basado en evidencia clínica nacional e internacional. Material y métodos: Este documento se desarrolló como una colaboración del Instituto Nacional de Cancerología y la Sociedad Mexicana de Oncología en cumplimiento con estándares internacionales. Se integró un grupo conformado por oncólogos médicos, cirujanos oncólogos, cirujanos de tórax, radio-oncólogos y metodólogos con experiencia en revisiones sistemáticas de la literatura y guías de práctica clínica. Resultados: Se consensaron, por el método Delphi y en reuniones a distancia, las recomendaciones en CPCP enfermedad extensa, producto de preguntas de trabajo. Se identificó y evaluó la evidencia científica que responde a cada una de dichas preguntas clínicas antes de incorporarla al cuerpo de la guía. Conclusión: Esta guía proporciona recomendaciones clínicas para el manejo de la enfermedad extensa del CPCP y durante el proceso de toma de decisiones de los clínicos involucrados con su manejo en nuestro país para mejorar la calidad de la atención clínica para estos pacientes.

Abstract Background: Small cell lung cancer (SCLC) represents 13-15% of all primary lung neoplasms and is characterized by its rapid growth rate and the rapid development of distant metastases. Objectives: To guide and standardize the treatment of extensive disease small cell lung cancer in Mexico based on national and international clinical evidence. Material and methods: This document was developed as a collaboration between the National Cancer Institute and the Mexican Society of Oncology in compliance with international standards. An interdisciplinary group was formed, including medical oncologists, oncological surgeons, thoracic surgeons, radiation oncologists, and methodologists with experience in systematic reviews of the literature and clinical practice guidelines. Results: A consensus was reached, both by the Delphi method and in remote meetings, of extensive disease recommendations resulting from work questions. The scientific evidence that answers each of these clinical questions was identified and critically evaluated, before being incorporated into the body of evidence of the Guide. Conclusions: This Clinical Practice Guide provides clinical recommendations for the management of extensive disease of SCLC to contribute to the decision-making process of the clinicians involved with its management in our country, hoping that this will contribute to improving the quality of clinical care in these patients.

https://doi.org/10.24875/j.gamo.22000122

Effect of sitagliptin on down-regulation of KAT7 and SIRT1 gene expression in breast cancer cell line MCF7
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Ruteaga-Navarro, Tanya C.

; Reyes-Romero, Miguel A.

; Torres-Salazar, Quitzia L.

Cirugía y cirujanos

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jun 2023, Volume 91 N. 3 Pages 334 - 338

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Antecedentes. Hasta la fecha, el principal interés clínico de la DPP4 se centra en su inhibición en pacientes diabéticos para prolongar la vida media de las incretinas. Las alteraciones epigenéticas resultantes de la inhibición de DPP4 han sido poco exploradas. Objetivo. Determinar si la sitagliptina, un inhibidor de DPP4, tiene efectos sobre la expresión de KAT7 y SIRT1 (genes que codifican una histona acetiltransferasa y una histona desacetilasa, respectivamente) en células de cáncer de mama MCF7, que desempeñan un papel esencial en la modulación del paisaje epigenético de la cromatina. Método. Las células MCF7 se incubaron durante 20 h con sitagliptina a concentraciones de 0.5, 1.0 y 2.0 μM. Se aisló el ARN total y se determinó la expresión relativa de ARNm de KAT7 y SIRT1 mediante RT-qPCR. Resultados. Hubo una regulación a la baja en la expresión relativa de ambos genes; para KAT7, la regulación negativa alcanzó hasta 0.49 (p = 0.027) y para SIRT1 alcanzó hasta 0.55 (p = 0.037). Conclusiones. Estos resultados sugieren que la sitagliptina tiene efectos sobre el paisaje epigenético de las histonas. Este tema merece más estudios debido al uso actual de inhibidores de DPP4 en pacientes diabéticos.

Abstract Background. To date, the main clinical interest in DPP4 is focused on its inhibition in diabetic patients to prolong the half-life of incretins. Epigenetic alterations resulting from DPP4 inhibition have been poorly explored. Objective. The objective of this study was to determine, whether sitagliptin, a DPP4 inhibitor, has effects on the expression of KAT7 and SIRT1 (genes encoding a histone acetyltransferase and a histone deacetylase, respectively) in MCF7 breast cancer cells, which play an essential role in modulating the epigenetic landscape of chromatin. Material and methods. MCF7 cells were incubated for 20 h with sitagliptin at concentrations of 0.5, 1.0 and 2.0 μM. Total RNA was isolated and the relative mRNA expression of KAT7 and SIRT1 was determined by RT-qPCR. Results. There was downregulation in the relative expression of both genes; for KAT7, downregulation reached up to 0.49 (p = 0.027) and for SIRT1, it reached up to 0.55 (p = 0.037). Conclusions. These results suggest that sitagliptin has effects on the histone epigenetic landscape. This topic deserves further study due to the current sample use of DPP4 inhibitors in diabetic patients.

https://doi.org/10.24875/ciru.22000189

Promoción de la lectura a través del cine durante la pandemia del covid-19
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Hernández Reyes, Julián Francisco

; Ojeda Ramírez, Mario Miguel

; Sánchez-Sosa, Eika Rubí

LiminaR

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jun 2023, Volume 21 N. 1 elocation e962

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen: En este artículo se presentan los resultados de una intervención de promoción de la lectura realizada en Xalapa, Veracruz, durante la pandemia de covid-19. Desde la perspectiva de la literacidad, el enfoque constructivista y el aprecio por la lectura a través del cine, los espectadores fueron guiados hacia géneros literarios diversos para favorecer la creación de lectores capaces de comprender críticamente lo que leen mediante inferencias basadas en su contexto personal. A través de una investigación-acción participativa, se desarrolló un taller con tres objetivos fundamentales: fomentar la lectura, mostrar la relevancia de la literatura en la cultura y sociedad y favorecer procesos de comprensión lectora. Se concluye que la dupla cine-literatura es factible para este propósito.

Abstract: This article discusses the results of a reading promotion activity carried out in Xalapa, Veracruz, Mexico, during the Covid-19 pandemic. Based on a literacy perspective, a constructivist approach, and the appreciation for reading through cinema, spectators were guided through diverse literary genres in order to encourage readers to critically understand what they read by referencing their own personal contexts. A workshop involving participatory-action research was undertaken with three fundamental objectives: promote reading, highlight literature’s relevance in culture and society, and promote reading comprehension. A conclusion derived from this exercise is that a film-literature duo can be useful in fulfilling the stated objectives.

https://doi.org/10.29043/liminar.v21i1.962

10.

Investigación clínica e industria farmacéutica
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Mimenza-Alvarado, Alberto J.

; Arrieta, Oscar

; Celis, Miguel A.

; Domínguez-Cherit, Judith

; Islas-Andrade, Sergio

; Lifshitz, Alberto

; Mansilla, Armando

; Martínez, Iris

; Moreno, Mucio

; Reyes-Sánchez, Alejandro A.

; Rocha-Arrieta, Luisa L.

; Argüelles, Guillermo J. Ruiz

; Sotelo, Julio

; Verástegui, Emma

; Vilar-Compte, Diana

; Toussaint, Sonia

Gaceta médica de México

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apr 2023, Volume 159 N. 2 Pages 91 - 92

Abstract: Es | Text: Es | PDF: Es

https://doi.org/10.24875/gmm.22000279

11.

Internal structure and invariance of a brief version of the Marijuana Motives Measure (MMM-P15) in a peruvian population of Marijuana users
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Vallejos-Flores, Miguel

; Capa-Luque, Walter

; Caycho-Caja, Aaron

; Chavez-Santamaria, Alexandra

; Reyes-Baca, Gino

; Bello-Vidal, Catalina

Anales de Psicología

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apr 2023, Volume 39 N. 1 Pages 39 - 50

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Background: The identification of the reasons for marijuana use has been supported by instruments such as the MMM. Despite its importance, there are still no valid brief versions of this instrument in Latin American countries. This paper seeks to fill this gap by providing evidence of validity and reliability, as well as the structural invariance of the MMM. Method: The sample consisted of 1164 male and female marijuana users, aged between 18 and 49 years, from Lima and Callao. Results: The factor analysis found a penta-factorial structure. The brief version (MMM-P15) and the wide MMM showed favorable properties from structure and reliability (ꙍ > .88, H > .84) with appropriate indicators of the short instrument adjustment (RMSEA = .058 (CI 90% .050, .067), SRMR = .044, TLI = .99, CFI = .99). Likewise, both versions maintain the invariance of the instrument according to sex and evidence of internal validity was found (AVE > .50). Aside from bringing evidence of validity with other constructs like CAST and SWLS scale. Conclusions: The MMM-P15 shows psychometric properties that support its use in both male and female marijuana users, and it is a short, versatile, and useful instrument for research purposes even in clinical settings.

Resumen Antecedentes: La identificación de los motivos por los cuales se consume marihuana se ha visto apoyada por instrumentos como el MMM. A pesar de su importancia, aún no se cuenta con versiones breves válidas de este en países latinoamericanos. Este trabajo busca cubrir esta carencia aportando evidencia de validez y confiabilidad, así como la invarianza estructural del MMM. Método: La muestra comprende 1164 consumidores de marihuana, varones y mujeres, con edades entre 18 y 49 años, de Lima y Callao. Resultados: El análisis factorial halló una estructura pentafactorial. La versión breve (MMM-P15) y extensa (MMM) muestran favorables propiedades de estructura y consistencia interna (ꙍ > .88, H > .84) con adecuados índices de ajuste del instrumento corto (RMSEA = .058 (IC 90% .050, .067), SRMR = .044, GFI = .99, TLI = .99, CFI = .99). Asimismo, ambas versiones mantienen la invarianza según el sexo y se encontró evidencia de validez interna (AVE > .50) y con otros constructos (CAST y SWLS). Conclusiones: El MMM-P15 evidencia propiedades psicométricas que respaldan su uso tanto en hombres y mujeres consumidores de marihuana, además es un instrumento corto, versátil y útil para fines de investigación incluso en contextos clínicos.

https://doi.org/10.6018/analesps.509651

12.

Cannabinoides y su uso terapéutico
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Islas-Andrade, Sergio

; Rocha-Arrieta, Luisa L.

; Arrieta, Oscar

; Celis, Miguel A.

; Domínguez-Cherit, Judith

; Lifshitz, Alberto

; Mansilla-Olivares, Armando

; Martínez, Iris

; Mimenza, Alberto José

; Moreno, Mucio

; Reyes-Sánchez, Alejandro A.

; Ruiz-Argüelles, Guillermo J.

; Soda-Merhy, Antonio

; Sotelo, Julio

; Toussaint, Sonia

; Vilar-Compte, Diana

; Verástegui, Emma

Gaceta médica de México

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feb 2023, Volume 159 N. 1 Pages 1 - 2

Abstract: Es | Text: Es | PDF: Es

https://doi.org/10.24875/gmm.22000184

13.

DNA sequencing in the classroom: complete genome sequence of two earwig (Dermaptera; Insecta) species
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Kobayashi, Sanae

; Maldonado, Jonathan E.

; Gaete, Alexis

; Araya, Ingrid

; Aguado-Norese, Constanza

; Cumplido, Nicolás

; Díaz, Sebastián

; Espinoza, Alonso

; Fernández, Edelmira

; Gajardo, Felipe

; González-Ordenes, Felipe

; Hauyon, Khantati

; Maldonado, Piedad

; Maldonado, Rodrigo

; Pochet, Isabel

; Riveros, Aníbal

; Sandoval, Paula

; Sepúlveda-González, Ailynne

; Stuardo, Camila

; Tapia-Reyes, Patricio

; Thornton, Carolina

; Undurraga, Soledad

; Varas, Macarena

; Valdivieso, Camilo

; Gutiérrez, Rodrigo A.

; Orellana, Ariel

; Montecino, Martín

; Maass, Alejandro

; González, Mauricio

; Allende, Miguel L.

; Hodar, Christian

; Irles, Paula

Biological Research

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2023, Volume 56 elocation 6

Abstract: En | Text: En | PDF: En

Abstract Background Despite representing the largest fraction of animal life, the number of insect species whose genome has been sequenced is barely in the hundreds. The order Dermaptera (the earwigs) suffers from a lack of genomic information despite its unique position as one of the basally derived insect groups and its importance in agroecosystems. As part of a national educational and outreach program in genomics, a plan was formulated to engage the participation of high school students in a genome sequencing project. Students from twelve schools across Chile were instructed to capture earwig specimens in their geographical area, to identify them and to provide material for genome sequencing to be carried out by themselves in their schools. Results The school students collected specimens from two cosmopolitan earwig species: Euborellia annulipes (Fam. Anisolabididae) and Forficula auricularia (Fam. Forficulidae). Genomic DNA was extracted and, with the help of scientific teams that traveled to the schools, was sequenced using nanopore sequencers. The sequence data obtained for both species was assembled and annotated. We obtained genome sizes of 1.18 Gb (F. auricularia) and 0.94 Gb (E. annulipes) with the number of predicted protein coding genes being 31,800 and 40,000, respectively. Our analysis showed that we were able to capture a high percentage (≥ 93%) of conserved proteins indicating genomes that are useful for comparative and functional analysis. We were also able to characterize structural elements such as repetitive sequences and non-coding RNA genes. Finally, functional categories of genes that are overrepresented in each species suggest important differences in the process underlying the formation of germ cells, and modes of reproduction between them, features that are one of the distinguishing biological properties that characterize these two distant families of Dermaptera. Conclusions This work represents an unprecedented instance where the scientific and lay community have come together to collaborate in a genome sequencing project. The versatility and accessibility of nanopore sequencers was key to the success of the initiative. We were able to obtain full genome sequences of two important and widely distributed species of insects which had not been analyzed at this level previously. The data made available by the project should illuminate future studies on the Dermaptera.

https://doi.org/10.1186/s40659-023-00414-9

14.

The role of echocardiographic screening in reducing the burden of rheumatic heart disease in Latin America
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Urina-Jassir, Manuel

; Jaimes-Reyes, Maria Alejandra

; Urina-Jassir, Daniela

; Urina-Triana, Manuel

; Urina-Triana, Miguel

Revista Panamericana de Salud Pública

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2023, Volume 47 elocation e158

Abstract: Es En Pt | Text: Es En Pt | PDF: Es | PDF: En | PDF: Pt

RESUMEN Los objetivos de este artículo son reflexionar sobre los fundamentos que justifican el uso del tamizaje ecocardiográfico para detectar la cardiopatía reumática y ofrecer algunas recomendaciones importantes sobre los pasos que habría que dar para poner en marcha programas de tamizaje ecocardiográfico y mejorar los existentes en América Latina. La cardiopatía reumática sigue siendo un problema de salud pública que afecta principalmente a países y grupos poblacionales de ingresos bajos y medianos bajos. América Latina es una región de grandes desigualdades económicas y las características epidemiológicas de la cardiopatía reumática siguen siendo desconocidas en gran medida. El tamizaje ecocardiográfico resulta útil para actualizar los datos epidemiológicos y posibilitar un diagnóstico temprano de la enfermedad. En este artículo se analizan los diferentes enfoques empleados en algunos programas de tamizaje ecocardiográfico eficaces de distintas partes del mundo, incluida América Latina. A continuación se determinan los elementos clave necesarios para establecer programas eficaces de tamizaje ecocardiográfico en América Latina, incluida una mayor concientización y participación de diversos sectores (p. ej., la comunidad, los profesionales de salud, las organizaciones científicas y las entidades de salud pública), la identificación de las zonas más necesitadas, la elaboración de un plan y una estructura que incluyan diferentes abordajes del tamizaje, y el modo de garantizar un seguimiento adecuado de aquellas personas con un resultado positivo en el tamizaje.

ABSTRACT The objectives of this article are to reflect on the rationale behind the use of echocardiographic screening for rheumatic heart disease and to provide key recommendations about steps needed to implement and improve echocardiographic screening programs in Latin America. Rheumatic heart disease remains a public health problem affecting mainly low-income and lower-middle-income countries and populations. Latin America is an area with economic inequalities, and the epidemiology of rheumatic heart disease remains largely unknown. Echocardiographic screening is useful for updating the epidemiology and providing early diagnosis of the disease. We discuss different approaches used in successful echocardiographic screening programs worldwide and in Latin America. We then identify the key elements needed to establish successful echocardiographic screening programs in Latin America, including increased awareness and involvement from multiple sectors (e.g. the community, health care professionals, scientific organizations and public health entities), identification of areas in need, development of a plan and structure that include different screening approaches, and how to ensure appropriate follow up for those who screen positive.

RESUMO Os objetivos deste artigo são oferecer observações sobre a fundamentação do uso da triagem ecocardiográfica para doença cardíaca reumática e fornecer recomendações importantes sobre as etapas necessárias para implementar e melhorar os programas de triagem ecocardiográfica na América Latina. A doença cardíaca reumática continua sendo um problema de saúde pública que afeta principalmente países e populações de renda baixa e média-baixa. A América Latina é uma área com desigualdades econômicas, e a epidemiologia da doença cardíaca reumática continua amplamente desconhecida. A triagem ecocardiográfica serve para atualizar a epidemiologia e proporcionar o diagnóstico precoce da doença. Examinamos as diferentes abordagens usadas em programas de triagem ecocardiográfica bem-sucedidos em todo o mundo e na América Latina. Em seguida, identificamos os principais elementos necessários para estabelecer programas de triagem ecocardiográfica com sucesso na América Latina. Tais programas incluiriam maior conscientização e envolvimento de vários setores (por exemplo, a comunidade, profissionais de saúde, organizações científicas e entidades de saúde pública), identificação de áreas carentes, desenvolvimento de um plano e estrutura abrangendo diferentes abordagens de triagem e formas de garantir o seguimento adequado de pessoas com resultado positivo na triagem.

https://doi.org/10.26633/rpsp.2023.158

15.

Pandemic grief in El Salvador: factors that predict dysfunctional grief due to a COVID-19 death among Salvadoran adults
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Lobos-Rivera, Marlon Elías

; Flores-Monterrosa, Angélica Nohemy

; Tejada-Rodríguez, Jennifer Carolina

; Chacón-Andrade, Edgardo René

; Caycho-Rodríguez, Tomás

; Lee, Sherman A.

; Valencia, Pablo D.

; Carbajal-León, Carlos

; Vilca, Lindsey W.

; Reyes-Bossio, Mario

; Gallegos, Miguel

Psicologia: Reflexão e Crítica

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2023, Volume 36 elocation 9

Abstract: En | Text: En | PDF: En

Abstract Thousands of people have died of COVID-19 in El Salvador. However, little is known about the mental health of those who are mourning the loss of a loved one to COVID-19. Therefore, the objective of this study was to examine the dysfunctional grief associated with COVID-19 death among Salvadoran adults. A sample of 435 Salvadorans ( M = 29 years; SD = 8.75) who lost a family member or loved one to COVID-19 completed a digital survey using the Google Forms platform, during April 2 and 28, 2022. The results revealed that 35.1% reported clinically elevated symptoms of dysfunctional grief and among those mourners, and 25.1% also exhibited clinical levels of coronavirus anxiety. A binary logistic regression revealed that predictor variables such as COVID-19 anxiety ( p = .003), depression ( p = .021), and COVID-19 obsession ( p = .032) were significant ( χ 2 = 84.31; Nagelkerke R 2 = .242) and predict a 24.2% chance of dysfunctional bereavement.

https://doi.org/10.1186/s41155-023-00250-6

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